CN1598542A - Instrument for measuring reflection ratio of biosample - Google Patents
Instrument for measuring reflection ratio of biosample Download PDFInfo
- Publication number
- CN1598542A CN1598542A CN 200410041858 CN200410041858A CN1598542A CN 1598542 A CN1598542 A CN 1598542A CN 200410041858 CN200410041858 CN 200410041858 CN 200410041858 A CN200410041858 A CN 200410041858A CN 1598542 A CN1598542 A CN 1598542A
- Authority
- CN
- China
- Prior art keywords
- laser
- integrating sphere
- sample
- perforation
- reflection ratio
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a detector for biology sample reflection rate, which includes a carrying platform, there arranges with standard sample and sample need to be measured in the carrying platform, its character lies in: the optical platform is arranged with a integral ball, the integral ball is arranged with a laser inlet aperture and a laser output aperture, one side of the inlet aperture is arranged with a reflection mirror, one side of the reflection mirror is arranged with a laser, the lower part of the outlet aperture is arranged with a photoelectric diode, the signal outputting wire of the photoelectric diode is connected to a computer. The invention can realize measurement without cover for the sample, the absolute error is less than 0.01 to 514nm, 633nm and 780nm.
Description
Technical field
The present invention relates to surface optics feature measurement instrument, specifically is a kind of surveying instrument of sample surfaces hemisphere reflectance.
Background technology
At present, the sample surfaces hemisphere reflectance of measuring certain material all is to carry out on spectrophotometer usually, general sample is all vertically put, and for the sample of shot shape or softness (as biological tissue), must cover transparent cover (being generally quartz glass) thereon and prevent that sample is unrestrained.So, the sample surfaces proterties suffers damage inevitably, thereby makes actual value when the result who measures departs from state of nature; On the other hand, laser at modern medicine, biomedical just obtaining a large amount of uses because the laser with polarization characteristic and general light source performance is different, make data unpredictable deviation of generation in laser is used of utilizing spectrophotometer to obtain.
For modern medicine, Biology Applications, though Chang Yong spectrophotometer also can record the hemisphere reflectance of sample surfaces more at present, as previously mentioned, its data that record will be subjected to certain restriction in actual applications.
Summary of the invention
In order to realize the unification of experimental data and application, use at modern medicine, biological laser, the invention provides a kind of is the biological sample reflection ratio measuring instrument of light source with laser, and employing keeps flat the hemisphere reflection ratio measuring that structures of samples realizes sample.
Technical scheme of the present invention is:
Biological sample reflection ratio measuring instrument, include objective table, place standard model and testing sample in the objective table, it is characterized in that being equipped with on the optical table integrating sphere, have laser on the integrating sphere and go into perforation and laser emitting hole, one side of going into perforation is equipped with a catoptron, one side of catoptron is equipped with laser instrument, and the below of perforation hole is placed with objective table, also has laser diffuse reflection hole on the integrating sphere, diffuse reflection is equipped with photodiode on the hole, and the photodiode signal output line connects computing machine.
Described biological sample reflection ratio measuring instrument is characterized in that described laser instrument is equipped with beam expanding lens in the light path of catoptron.
Described biological sample reflection ratio measuring instrument, it is characterized in that described laser instrument installs semi-transparent-half anti-mirror in the light path of catoptron, the below of semi-transparent-half anti-mirror is equipped with the diffusion trap, in the side perforate of diffusion trap photodiode is installed, and the photodiode signal output line connects computing machine.
Described biological sample reflection ratio measuring instrument is characterized in that described laser goes into perforation and perforation hole on the radial line of integrating sphere, and described objective table is installed on the chute and slide block mechanism on the optical table, can slide with slide block.
Each parts of the present invention are to adopt support or box body to be installed on the optical table according to the needs of light path, optical table is the base plate of iron or steel, objective table is mounted on chute and the slide block mechanism, chute is installed on the optical table of below, integrating sphere laser emitting hole, objective table is installed on the slide block, can slide with slide block.
Its principle of work is: the light that laser instrument sends passes through semi-permeable and semi-reflecting mirror after expanding bundle through beam expanding lens again, and the light of reflection is transferred to computing machine by silicon photoelectric diode with photosignal through the diffusion trap; Light through transmission passes through catoptron again, injects in the integrating sphere, shines on the standard model and biological sample to be measured of integrating sphere below, by silicon photoelectric diode photoelectron signal is transferred to computing machine, carries out the optics computing.Measure the reflectance of biological sample at last.
Biological sample reflection ratio measuring instrument of the present invention is made up of sample test unit and laser surveillance unit and computer data acquiring display part, is used for measuring the hemisphere reflectance of biological sample.
Wherein, integrating sphere, silicon photoelectric diode, objective table and standard model have constituted the sample test unit; Diffusion trap and silicon photoelectric diode then constitute the laser power monitor unit, are used for monitoring the laser power drift of incident in sample and twice measuring process of standard model.Computer unit is used for gathering, showing and transmit the hemispherical reflectance of sample.Wherein, can change the sample reflection ratio measuring that different wavelength of laser light source and photodetector are realized the different wave length place according to the needs of using.
The present invention utilizes the integrating sphere principle, uses to substitute the method for measuring, and obtains the hemispherical reflectance of sample by the contrast of sample and standard model.
According to the integrating sphere principle, measurement standard sample at first, integrating sphere exports the luminous flux of detector to:
Secondly, measure testing sample, integrating sphere exports the luminous flux of detector to:
In the formula, A is the detector place, the integrating sphere aperture area,
Φ
IncidentIncident flux when measuring for standard model,
Φ '
IncidentIncident flux when measuring for testing sample.
(1), (2) two formulas compare:
At this, ρ
The ball wallBe the reflectivity of integrating sphere inwall,
Be total perforate equivalence factor, f
SampleBe the sample perforate equivalence factor, ρ
StandardBe the standard model reflectivity.
Because therefore the output voltage V ∝ Φ of silicon photoelectric diode, substitutes the hemispherical reflectance that Φ corresponding in the following formula can try to achieve sample with variant V.
Biological sample reflection ratio measuring instrument of the present invention can not realized measuring under the situation of enrobe at sample surfaces, and the light source of its use can reach consistent with the light source that reality is used.Through to the detection of standard model, all obtained absolute deviation less than 0.01 measuring accuracy at 514nm, 633nm and 780nm.
Description of drawings
Accompanying drawing is a structural representation of the present invention.
Embodiment
Biological sample reflection ratio measuring instrument, include optical table 14, chute and slide block mechanism 12 are arranged on the optical table 14, on the slide block of chute and slide block mechanism 12 objective table is installed, place standard model 11 and testing sample 10 in the objective table, an integrating sphere 1 is installed on the optical table 14, integrating sphere 1 upper end has laser and goes into perforation, the lower end has the laser emitting hole, and a side of going into perforation is equipped with one 45 degree plane mirrors 9, and laser instrument 6 is installed on the optical table 14, along in the laser emitting direction light path of laser instrument 6, beam expanding lens group 7 also is installed, semi-transparent-half anti-mirror 8 of 45 degree is installed in the subsequent optical path of beam expanding lens group 7, the laser toward mirror 9 then.In the reflected light path of semi-transparent-half anti-mirror 8, the below of semi-transparent-half anti-mirror 8 is equipped with diffusion trap 4, diffusion trap 4 sides have Si photodiode 3 are installed on the hole, Si photodiode 3 output lines connect computing machine 5, below perforation hole, be placed with objective table, integrating sphere 1 side also has laser diffuse reflection hole, and visual diaphragm 13 of photodiode and Si photodiode 2 are installed on the diffuse reflection hole, and photodiode 2 output lines connect computing machine.Described laser is gone into perforation and perforation hole on the radial line of integrating sphere.
Claims (4)
1, biological sample reflection ratio measuring instrument, include objective table, place standard model and testing sample in the objective table, it is characterized in that being equipped with on the optical table integrating sphere, have laser on the integrating sphere and go into perforation and laser emitting hole, one side of going into perforation is equipped with a catoptron, one side of catoptron is equipped with laser instrument, the below of perforation hole is placed with objective table, also have laser diffuse reflection hole on the integrating sphere, diffuse reflection is equipped with photodiode on the hole, and the photodiode signal output line connects computing machine.
2, biological sample reflection ratio measuring instrument according to claim 1 is characterized in that described laser instrument is equipped with beam expanding lens in the light path of catoptron.
3, biological sample reflection ratio measuring instrument according to claim 1, it is characterized in that described laser instrument installs semi-transparent-half anti-mirror in the light path of catoptron, the below of semi-transparent-half anti-mirror is equipped with the diffusion trap, in the side perforate of diffusion trap photodiode is installed, the photodiode signal output line connects computing machine.
4, biological sample reflection ratio measuring instrument according to claim 1 is characterized in that described laser goes into perforation and perforation hole on the radial line of integrating sphere, and described objective table is installed on the chute and slide block mechanism on the optical table, can slide with slide block.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100418589A CN100367026C (en) | 2004-08-31 | 2004-08-31 | Instrument for measuring reflection ratio of biosample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100418589A CN100367026C (en) | 2004-08-31 | 2004-08-31 | Instrument for measuring reflection ratio of biosample |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1598542A true CN1598542A (en) | 2005-03-23 |
| CN100367026C CN100367026C (en) | 2008-02-06 |
Family
ID=34665285
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100418589A Expired - Fee Related CN100367026C (en) | 2004-08-31 | 2004-08-31 | Instrument for measuring reflection ratio of biosample |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100367026C (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1920510B (en) * | 2006-07-07 | 2010-04-21 | 浙江大学三色仪器有限公司 | Daylight contrast measuring apparatus for flat panel display device |
| CN101923000A (en) * | 2010-07-13 | 2010-12-22 | 中国兵器工业第二〇五研究所 | Optical measuring device with high reflectivity and high transmissivity |
| CN103097877A (en) * | 2010-07-21 | 2013-05-08 | 阿文戈亚太阳能新技术公司 | Portable reflectometer and method for characterising the mirrors of solar thermal power plants |
| CN103234912A (en) * | 2013-04-17 | 2013-08-07 | 中国工程物理研究院流体物理研究所 | Apparatus and method for dynamically measuring material laser reflectivity under supersonic tangential gas flow |
| CN105527252A (en) * | 2016-01-13 | 2016-04-27 | 中国工程物理研究院激光聚变研究中心 | Optical element reflectivity measurement instrument |
| CN107843562A (en) * | 2017-12-08 | 2018-03-27 | 湖南文理学院 | A kind of integrating sphere device and integration sphere light source system |
| CN108061707A (en) * | 2017-12-08 | 2018-05-22 | 湖南文理学院 | A kind of integration sphere light source system and test method |
| CN107389600B (en) * | 2017-08-14 | 2019-08-06 | 江苏特丰新材料科技有限公司 | Near-infrared reflection ratio, transmittance and the determination of absorption ratio method of coating |
| CN113607663A (en) * | 2021-07-06 | 2021-11-05 | 武汉理工大学 | Diffuse reflection type multipurpose multivariable coupling in-situ optical absorption testing device and method |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US487504A (en) * | 1892-12-06 | Bale-tie | ||
| AU644518B2 (en) * | 1991-04-29 | 1993-12-09 | Labsphere, Inc. | Integrating sphere for diffuse reflectance and transmittance measurements and the like |
| US5517315A (en) * | 1993-10-29 | 1996-05-14 | The United States Of America As Represented By The Secretary Of The Navy | Reflectometer employing an integrating sphere and lens-mirror concentrator |
| CN2432578Y (en) * | 2000-06-12 | 2001-05-30 | 中国科学院安徽光学精密机械研究所 | Optical integrating-sphere |
-
2004
- 2004-08-31 CN CNB2004100418589A patent/CN100367026C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1920510B (en) * | 2006-07-07 | 2010-04-21 | 浙江大学三色仪器有限公司 | Daylight contrast measuring apparatus for flat panel display device |
| CN101923000A (en) * | 2010-07-13 | 2010-12-22 | 中国兵器工业第二〇五研究所 | Optical measuring device with high reflectivity and high transmissivity |
| CN101923000B (en) * | 2010-07-13 | 2012-07-25 | 中国兵器工业第二〇五研究所 | Optical measuring device with high reflectivity and high transmissivity |
| CN103097877A (en) * | 2010-07-21 | 2013-05-08 | 阿文戈亚太阳能新技术公司 | Portable reflectometer and method for characterising the mirrors of solar thermal power plants |
| CN103097877B (en) * | 2010-07-21 | 2016-01-20 | 阿文戈亚太阳能新技术公司 | Portable Reflectance for measuring mirror characteristic in solar thermal power plants takes into account its method of operating |
| CN103234912A (en) * | 2013-04-17 | 2013-08-07 | 中国工程物理研究院流体物理研究所 | Apparatus and method for dynamically measuring material laser reflectivity under supersonic tangential gas flow |
| CN103234912B (en) * | 2013-04-17 | 2015-06-03 | 中国工程物理研究院流体物理研究所 | Apparatus and method for dynamically measuring material laser reflectivity under supersonic tangential gas flow |
| CN105527252A (en) * | 2016-01-13 | 2016-04-27 | 中国工程物理研究院激光聚变研究中心 | Optical element reflectivity measurement instrument |
| CN107389600B (en) * | 2017-08-14 | 2019-08-06 | 江苏特丰新材料科技有限公司 | Near-infrared reflection ratio, transmittance and the determination of absorption ratio method of coating |
| CN107843562A (en) * | 2017-12-08 | 2018-03-27 | 湖南文理学院 | A kind of integrating sphere device and integration sphere light source system |
| CN108061707A (en) * | 2017-12-08 | 2018-05-22 | 湖南文理学院 | A kind of integration sphere light source system and test method |
| CN113607663A (en) * | 2021-07-06 | 2021-11-05 | 武汉理工大学 | Diffuse reflection type multipurpose multivariable coupling in-situ optical absorption testing device and method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100367026C (en) | 2008-02-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102121818B (en) | Method and device for measurement of nanometer resolution total reflection differential micrometric displacement | |
| US8587786B2 (en) | Method for high-resolution detection of nanoparticles on two-dimensional detector surfaces | |
| FR2738343A1 (en) | Optical microstratigraphy equipment | |
| US20080144006A1 (en) | Method for Measuring Topographic Structures on Devices | |
| CN101017083A (en) | High-density multi-channel testing device | |
| SE1150497A1 (en) | Surface Plasma Resonance Sensor Utilizing Radiation Profile Lipsometry | |
| CN1598542A (en) | Instrument for measuring reflection ratio of biosample | |
| JP2007248448A (en) | Internal structure inspecting method and internal structure inspecting device for pearl or precious stones | |
| US5715059A (en) | Dark field, photon tunneling imaging systems and methods | |
| Harlepp et al. | Subnanometric measurements of evanescent wave penetration depth using total internal reflection microscopy combined with fluorescent correlation spectroscopy | |
| Golnabi | Surface profiling using a double-fiber optical design | |
| CN107991233A (en) | Noble metal nano array extinction spectra measuring device and its sensing detection method | |
| US8656510B1 (en) | System, apparatus, and method for simultaneous single molecule atomic force microscopy and fluorescence measurements | |
| Zhou et al. | Fiber-optic refractometer based on a reflective aspheric prism rendering adjustable sensitivity | |
| CN1233985C (en) | Aligning linkage precision detecting system | |
| TW201341845A (en) | Three-dimensional microscope device and method using the same | |
| JP5992990B2 (en) | Objective optical system and ATR measurement apparatus for ATR measurement | |
| CN114184583A (en) | Optical measurement and evaluation method for depth consistency of high-aspect-ratio microstructure | |
| CN2672648Y (en) | Portable near infrared optical fiber micro detector for quick on-line detecting material property | |
| JP2003075764A (en) | Apparatus for determining light power level, microscope, and method for microscopy | |
| CN101008609B (en) | Optical waveguide biological detecting device | |
| Muttalib et al. | Experimental measurement of photonic/plasmonic crystal dispersion, applied to the investigation of surface plasmon dispersion for SERS sensing applications | |
| CN1587992A (en) | Micro suspension arm beam driven light intensity modulated type optic-fiber hydrogen sensor | |
| CN109443241A (en) | A kind of high speed axial direction scanning confocal micro-measurement apparatus and method based on tuning fork driving | |
| CN218329795U (en) | Microscope film thickness measuring platform |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |