CN1579252A - Compound-acid drink and its preparation method - Google Patents
Compound-acid drink and its preparation method Download PDFInfo
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- CN1579252A CN1579252A CN 03149691 CN03149691A CN1579252A CN 1579252 A CN1579252 A CN 1579252A CN 03149691 CN03149691 CN 03149691 CN 03149691 A CN03149691 A CN 03149691A CN 1579252 A CN1579252 A CN 1579252A
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Abstract
The invention relates to a compound acid beverage, the manufacturing method is: A. the invention cultures lactic acid bacteria, acetic acid bacteria and yeast bacteria, acquires the cultured product; B. the Chinese date with content of 1- weight shares, Gynostemma penfaphylla 0.1-3 shares, hsueh-lien-hua 0.1-3 shares, gadol 0.1-10 shares and maidenhair tree shares, they are stewed for 1-4 times with 3-20 times water, acquires the extracted liquid, then they are blended with 2-8 shares of sugar, 1-4 shares of honeydew, 1-4 iso malt oligosaccharide, produces the compound material for yeasting; C. the cultured product in step A is inoculated to the compound material in step B, yeasted in 25-37 deg.C and pH 3-6 for 2-10 days; D. the liquid after step C is processed into beverage. The invention has protection effect to virosis such as pneumonia, hepatitis, rheum and so on, resists several kinds of viruses and bacteria and epiphyte infection, and it has resisting effect to SARS virus.
Description
Invention field
The present invention relates to a kind of compound acid beverage, it is a kind of biofermentation type beverage, is made by Chinese medicine extract inoculation acetic acid bacteria, saccharomycete and lactobacillus-fermented.
Background technology
Modern society competition is growing more intense, and people are because operating pressure and stress and health status descends day by day.The Chinese patent communique discloses the many health drinks that are used for these purposes.These beverages have satisfied people's demand to a certain extent.But improving and act on more fully for effect, health drink still exists lasting surging demand.
Summary of the invention
Based on this, the purpose of this invention is to provide a kind of compound acid beverage, this beverage is suitable for all groups, has antiviral and bacterium, improves immunity, regains one's strength, and antifatigue is improved sleep, the anti-ageing various health-care of waiting for a long time.
Compound acid beverage of the present invention is the biofermentation type beverage of being made by Chinese medicine extract inoculation acetic acid bacteria, saccharomycete and lactobacillus-fermented, and the preparation process of this beverage is as follows:
A, cultivation lactic acid bacteria, acetic acid bacteria and saccharomycete obtain cultured products.
The nutrient solution of wherein cultivating usefulness generally comprises nitrogenous source, and carbon source and water, nitrogenous source generally are selected from the skim milk dusty yeast, soybean protein, and inorganic salt containing nitrogen etc. or their mixture, carbon source comprises plant amylum, carbohydrate, organic compound etc.For example, this nutrient solution can comprise skim milk 80-150 weight portion, glucose 80-150 weight portion, dusty yeast 7-12 weight portion, calcium carbonate 15-25 weight portion, agar 10-20 weight portion, brewer's wort 60-140 weight portion+agar 1-3 weight portion and water (preferred distilled water) 900-1600 weight portion, pH3-6.8, incubation time for example 24-72 hour also can be longer or shorter than this scope.Three kinds of bacterium can be cultivated separately, also can Mixed culture.
B, with date 1-10 weight portion, Gynostemma pentaphylla 0.1-3 weight portion, saussurea involucrata 0.1-3 weight portion, rhodiola root 0.1-10 weight portion, honeysuckle 0.1-3 weight portion and gingko 0.1-3 weight portion boil 1-4 time with the decocting that 3-20 doubly measures, and obtain extract, again with 2-8 weight portion sugar, 1-4 weight portion honey, 1-4 weight portion isomalto-oligosaccharide 1-4 weight portion mixes, and makes the fermentation compound.
C, three kinds of cultured products mixing of steps A are back or independent, or the fermentation that the mixed-culture medium of three kinds of bacterium is inoculated into step B is fermented with in the compound.Inoculum concentration is 1-7%, and based on the gross weight of three kinds of nutrient solutions and the part by weight of extract, wherein the weight proportion of three kinds of cultured products is 0.5-1.5: 1.5-2.5: 0.5-1.5.Fermentation temperature is 25-37 ℃, and preferably about 31-33 ℃, pH3.0-6.5, preferably about 4.0-4.3, fermentation time are 2 days to 10 days, preferred about 3 days (72 hours).
D, the mash after the step C fermentation is made beverage.
Step D for example can comprise coarse filtration, allotment (adjusting sugar, pH etc.), homogeneous, the smart filtration, sterilization, cooling, aseptic canning, packing.Also can only filter, sterilize, steps such as aseptic canning.
Compound acid of the present invention is a kind of multi-function health-care product, mainly by natural activity immune substance, health nutrient, three parts such as dietary fiber are formed, form to contain through acetic acid bacteria, saccharomycete and lactic acid bacteria symbiotic fermentation and derive from raw material and flora metabolism and produce multiple material to the human body beneficial, these materials make human physiological functions reach dynamic equilibrium effectively.Compound acid can be eaten the medicine dual-purpose, is a kind of desirable natural fermented type health beverages.
High energy mixture such as multivitamin, protein, amino acid, ribonucleic acid, maltol, glutathione, organic acid, inorganic acid, enzyme material, acetylcholine, ablastins, mineral matter, lactone material, polysaccharide, Polyphenols, purine base, cromoci, atriphos are rich in compound acid.
Function comprises: (1) promotes the synthetic of hereditary material DNA and RNA, strengthens metabolic function and enzymatic activity, and nervous system and physiological function are had bidirectional modulation.(2) preservation antioxidation, removing free radical; (3) antiviral, antibacterium and fungal infection; (4) anti-anoxic, antifatigue, radioresistance, enhancing immunity of organism, improve gastrointestinal function; (5) to viral disease as: disease infections such as pneumonia, hepatitis, flu have preventive and therapeutic effect, suppress the infection of multiple virus and bacterium fungi, especially SARS virus are had complete inhibitory action.
Compound acid of the present invention is a kind of multifunctional and composite type natural biological fermented health-care type beverage, and it is not only relieved summer heat and quenches one's thirst, again can disease-prevention health, and also be a kind of sharp weapon of opposing modern civilization disease, be applicable to all groups.
Specific embodiment
Come by the following examples further to set forth the present invention, it should be understood that these embodiment only are used for the purpose of illustration, never constitute limitation of the scope of the invention.
Embodiment 1
A, with the 100g skimmed milk power, 100g glucose, 10g dusty yeast, 20g calcium carbonate, 15g agar, 100ml brewer's wort+2g agar and 1000ml distilled water mix, and sterilize 15 minutes down at 120 ℃, make nutrient solution (pH6.8), prepare 3 parts altogether, each part adds 2ml lactic acid bacteria, acetic acid bacteria and saccharomycete (every ml contains the about 108-1010 of bacterium) respectively, cultivated 2 days, and obtained nutrient solution.
B, with date 200g, Gynostemma pentaphylla 30g, saussurea involucrata 30g, rhodiola root 30g, honeysuckle 30g and gingko 30g boil 2 times with the decocting of 6 times of amounts, obtain extract, again with sugared 500g, honey 200g, malto-oligosaccharide (the global group of Yucheng, Shandong produces) 20g mixes, and makes the fermentation compound.
C, with three kinds of nutrient solutions of steps A by acetic acid bacteria: saccharomycete: lactic acid bacteria=1: 2: 1 mixes fermentation that the back is inoculated into step B with 5% amount with in the compound, ferments under the pH at 4.0-4.3 under 32 ℃ 72 hours.
D, with the mash after the step C fermentation by the coarse filtration of beverage preparation conventional method, allotment then, homogeneous, the smart filtration, sterilization, cooling, aseptic canning is made beverage.
Embodiment 2
A, with the 100g skimmed milk power, 100g glucose, 10g dusty yeast, 20g calcium carbonate, 15g agar, 100ml brewer's wort+2g agar and 1000ml distilled water mix, and sterilize 15 minutes down at 120 ℃, make nutrient solution (pH6.8), prepare 3 parts altogether, each part adds 2ml lactic acid bacteria, acetic acid bacteria and saccharomycete (every ml contains the about 108-1010 of bacterium) respectively, cultivated 2 days, and obtained nutrient solution.
B, with date 250g, Gynostemma pentaphylla 25g, saussurea involucrata 35g, rhodiola root 40g, honeysuckle 20g and gingko 25g boil 3 times with the decocting of 4 times of amounts, obtain extract, again with sugared 400g, honey 250g, malto-oligosaccharide (the global group of Yucheng, Shandong produces) 25g mixes, and makes the fermentation compound.
C, with three kinds of nutrient solutions of steps A by acetic acid bacteria: saccharomycete: lactic acid bacteria=1: 2: 1 mixes fermentation that the back is inoculated into step B with 6% amount with in the compound, ferments under the pH at 4.0-4.3 under 33 ℃ 72 hours.
D, with the mash after the step C fermentation by the coarse filtration of beverage preparation conventional method, allotment then, homogeneous, the smart filtration, sterilization, cooling, aseptic canning is made beverage.
The effect test of beverage of the present invention
One, external SARS resisting medicine primary dcreening operation test
(1) experiment material
1, cell: Vero E6 cell (cercopithecus aethiops cell)
2, virus: SARS virus, BJ-01 strain
3, nutrient solution: the DMEM nutrient solution that contains 10% hyclone
(2) experiment condition
Three grades of laboratories of bio-safety
(3) experimental procedure
1, the CPE method is measured virus to Vero E6 cell half toxic concentration (TCID50)
2, the CPE method is determined the non-toxic concn of medicine pair cell in conjunction with mtt assay
3, the maximal non-toxic concentration of medicine is to the evaluation of pesticide effectiveness of SARS virus
(4) experimental result
1, viral TCID50 is 10-7
2, medicine was got 4 concentration 1: 100, and 1: 1000, carried out CTA in 1: 10000 and 1: 100000, the cell contrast is established in 4 multiple holes of every concentration simultaneously.The result shows that maximal non-toxic concentration is 1: 100.
3, get 1: 100 soup, do 4 multiple holes, observe inhibitory action SARS virus.Virus control and cell contrast are established in test.The result shows that this concentration medicine has complete inhibitory action to SARS virus.
(5) conclusion
Under this experimental condition, compound acid is 1: 100 dilution factor to the non-toxic concn of Vero E6 cell, and this concentration has complete inhibitory action to SARS virus.
Two, compound sour trial state for example
Slowly * *, man, 48 years old, fever and cough 3 days, Chest X-rays prompting bottom right pneumonia, blood leukocytes batch total 11000, neutrality 79%, lymph 21%.Body heat (38.6%) when examining, the pharyngalgia that is slightly cold, the sticking in vain difficulty of expectoration goes out, right lung audible and tiny bubble, the thin lingual fur Huang, superficial and rapid pulse, four methods of diagnosis mouth ginseng, intend belonging to the syndrome of disease involving weifen and qifen of rheumatism, take after compound sour 2 days body temperature and reduce to normally, cough also obviously reduces, the Chest X-rays foci disappearance, took every day 3 days, cured in 7 days.
Zhao *, man, 46 years old, diet does not advance when coming institute, and vomiting is frequent, haves the fidgets, shape is thin as bavin, skin sclera degree of depth xanthochromia, ascites (+), button pain (+), the liver upper bound six ribs, 1cm under the rib, belong to I °, icterus index 120 units, glutamic-pyruvic transaminase 97 units, prothrombin time are diagnosed as the B-mode severe subacute of virus hepatitis.Take every day 3 times, the state of an illness alleviates after 1 week, and jaundice is gradually moved back after 15 days, can advance a spot of diet, after 1 month, and transference cure, the energy out-of-bed activity, spirit is good, and complexion is preferable, and body weight increases, and liver function recovery is normal after 2 months, fully recovers after 3 months.
Once *, the woman, 23 years old, (38.3 ℃) stomachache that begins to have a headache and fever, abdominal distension, borborygmus, it is contrary to vomit, and surplus stool diarrhea every day 10 time, obey 2 compound acid heats and moves back, the contrary all disease disappearances and healing of suffering from diarrhoea, vomit.
The king *, the man, 47 years old, the gas that rises for many years, liver depression is not relaxed, indigestion.Cured vicennial disease through taking two weeks.
Horse * *, the woman 36 years old, suffers from for many years pain in the back and endocrine disorder, recovery from illness after drinking 1 month.
Slowly * *, the woman, 23 years old, face length had a lot of acne, all disappeared after drinking one month.
The king * *, the man, 77 years old, suffer from atrophic gastritis for many years, taken many kinds of medicines and do not run affairs, more and more serious on the contrary, old man's readme is taken medicine too much, and stomach can't bear.Drink the present invention after compound sour two months, the appetite body weight increases, and sleep improves, and stool is normal, and heart disease is not sent out again.
Zhao * *, the man 75 years old, suffered from atrophic gastritis 3 years, make gastroscope and make a definite diagnosis, it is cancer of the stomach that the traditional Chinese medical science is suspected, drinks the compound sour wheat harvesting period stomach of the present invention no longer sour regurgitation and cusalgia, and appetite increases, originally can not eat hard food, and can eat hard slightly food now, hypertension and heart disease also take a turn for the better to some extent.
Wu *, the man, 31 years old, former physique was very weak, and four seasons common cold is dizzy often, low myopia and astigmatism is arranged, diseases such as alopecia.After drinking " compound acid " some months, physique strengthens, and seldom catches a cold, and appetite increases, and is physically fit, in high spirits, of pink and healthy-looking, and normal overtime work is also unconsciously tired, and hair is pitch-black, and alopecia reduces, and eyesight has raising.
Sweet *, the man, 67 years old, former trouble obesity and senile chronic bronchitis etc., drink compound sour three months after, fat-reducing 5kg, bronchitis of old people is not sent out a winter again, now appetite is good, of pink and healthy-looking, in high spirits.
Open *, woman, 50 years old, former trouble diabetes, through treatment state of an illness basic controlling, but unstable, off color, drink " compound acid " (every day 4 times after two months, about each 50ml), check result, blood sugar is reduced to 120mg/100ml by original 290, cholesterol reduces to 184 by original 378, and glucose in urine reduces to 0 by 4 original plus siges.Body weight increases, now stopped using phenformin and other medicines.
Sieve *, man, 65 years old, suffer from high blood pressure, normal dizzy, it is bad to sleep, long blood knurl that beans are big in the nasal cavity of a left side, normal hemorrhage influence is breathed, and doctor's suggestion is had an operation, after drink " compound acid " four wheat harvesting periods, blood pressure reduces to 150/90 by 170/110, and is not dizzy now, sleep, nosebleed, the atrophy of blood knurl need not be eaten any Chinese and Western medicine.
Claims (5)
1, a kind of compound acid beverage is characterized in that being made by following steps:
A, cultivation lactic acid bacteria, acetic acid bacteria and saccharomycete obtain cultured products;
B, with date 1-10 weight portion, Gynostemma pentaphylla 0.1-3 weight portion, saussurea involucrata 0.1-3 weight portion, rhodiola root 0.1-10 weight portion, honeysuckle 0.1-3 weight portion and gingko 0.1-3 weight portion boil 1-4 time with the decocting that 3-20 doubly measures, and obtain extract, again with sugared 2-8 weight portion, honey 1-4 weight portion, isomalto-oligosaccharide 1-4 weight portion mixes, and makes the fermentation compound;
C, the cultured products of steps A is inoculated into step B fermentation with in the compound, 25-37 ℃ and pH3-6 bottom fermentation 2-10 days;
D, the mash after the step C fermentation is made beverage.
2, according to the compound acid beverage of claim 1, the nutrient solution that it is characterized in that step (A) comprises skim milk 80-150 weight portion, glucose 80-150 weight portion, dusty yeast 7-12 weight portion, calcium carbonate 15-25 weight portion, agar 10-20 weight portion, brewer's wort 60-140 weight portion+agar 1-3 weight portion and distilled water 900-1600 weight portion.
3,, it is characterized in that in step C the cultured products of steps A is inoculated into the fermentation of step B with in the compound with the amount of 1-7% according to the compound acid beverage of claim 1.
4, according to the compound acid beverage of claim 1, the weight proportion that it is characterized in that three kinds of cultured products is an acetic acid bacteria: saccharomycete: lactic acid bacteria=1: 2: 1.
5, the preparation method of compound acid beverage may further comprise the steps:
A, cultivation lactic acid bacteria, acetic acid bacteria and saccharomycete obtain cultured products;
B, with date 1-10 weight portion, Gynostemma pentaphylla 0.1-3 weight portion, saussurea involucrata 0.1-3 weight portion, rhodiola root 0.1-10 weight portion, honeysuckle 0.1-3 weight portion and gingko 0.1-3 weight portion boil 1-4 time with the decocting that 3-20 doubly measures, and obtain extract, again with sugared 2-8 weight portion, honey 1-4 weight portion, isomalto-oligosaccharide 1-4 weight portion mixes, and makes the fermentation compound;
C, the cultured products of steps A is inoculated into step B fermentation with in the compound, 25-37 ℃ and pH3.0-6.5 bottom fermentation 2-10 days;
D, the mash after the step C fermentation is made beverage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03149691 CN1579252A (en) | 2003-08-06 | 2003-08-06 | Compound-acid drink and its preparation method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 03149691 CN1579252A (en) | 2003-08-06 | 2003-08-06 | Compound-acid drink and its preparation method |
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| CN1579252A true CN1579252A (en) | 2005-02-16 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100336466C (en) * | 2005-11-14 | 2007-09-12 | 李应州 | Liquor with fructus momordicae |
| CN101326934B (en) * | 2008-07-25 | 2012-01-11 | 贵州大学 | Saussurea involucrata fruit nourishing composite milk and method for producing the same |
| CN102488063A (en) * | 2011-12-28 | 2012-06-13 | 贵州神奇集团控股有限公司 | Health preserving jujube containing Saussurea involucrata and preparation method thereof |
| CN103637310A (en) * | 2013-11-29 | 2014-03-19 | 李伟杰 | Healthy beverage and preparation method thereof |
| CN104774739A (en) * | 2015-04-29 | 2015-07-15 | 合肥不老传奇保健科技有限公司 | Aged edible cactus and gold silk jujube vinegar and preparation method thereof |
| CN107048361A (en) * | 2017-04-12 | 2017-08-18 | 塔里木大学 | A kind of big rue granatum composite enzyme of jujube and preparation method thereof |
-
2003
- 2003-08-06 CN CN 03149691 patent/CN1579252A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100336466C (en) * | 2005-11-14 | 2007-09-12 | 李应州 | Liquor with fructus momordicae |
| CN101326934B (en) * | 2008-07-25 | 2012-01-11 | 贵州大学 | Saussurea involucrata fruit nourishing composite milk and method for producing the same |
| CN102488063A (en) * | 2011-12-28 | 2012-06-13 | 贵州神奇集团控股有限公司 | Health preserving jujube containing Saussurea involucrata and preparation method thereof |
| CN102488063B (en) * | 2011-12-28 | 2015-06-17 | 贵州神奇集团控股有限公司 | Health preserving jujube containing Saussurea involucrata and preparation method thereof |
| CN103637310A (en) * | 2013-11-29 | 2014-03-19 | 李伟杰 | Healthy beverage and preparation method thereof |
| CN104774739A (en) * | 2015-04-29 | 2015-07-15 | 合肥不老传奇保健科技有限公司 | Aged edible cactus and gold silk jujube vinegar and preparation method thereof |
| CN107048361A (en) * | 2017-04-12 | 2017-08-18 | 塔里木大学 | A kind of big rue granatum composite enzyme of jujube and preparation method thereof |
| CN107048361B (en) * | 2017-04-12 | 2020-09-22 | 塔里木大学 | Red date and herba cistanches pomegranate rind composite enzyme and preparation method thereof |
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