CN1557943A - Bacterium culture medium and preparation method therefor - Google Patents
Bacterium culture medium and preparation method therefor Download PDFInfo
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- CN1557943A CN1557943A CNA2004100121360A CN200410012136A CN1557943A CN 1557943 A CN1557943 A CN 1557943A CN A2004100121360 A CNA2004100121360 A CN A2004100121360A CN 200410012136 A CN200410012136 A CN 200410012136A CN 1557943 A CN1557943 A CN 1557943A
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- nutrient agar
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Abstract
The present invention relates to bacteria culture medium, and is especially one kind of bacteria culture medium with nutritious agar culture medium as basic culture medium and its preparation process. The basic culture medium contains nutritious agar, water, water preserving agent and dye in the weight ratio of 36-40 to 1000 to 0.1-5 to 0.01-0.1 and is prepared through mixing in the weight proportion and sterilizing. The present invention has the features of convenient observation and detection, simple preparation process and convenient operation.
Description
Technical field
The present invention relates to a kind of bacteria culture medium and preparation method thereof, particularly a kind of is the bacteria culture medium and preparation method thereof of basic medium with the nutrient agar.
Background technology
At present, the bacteria culture medium major part is matrix with agar in the prior art, and adds the required nutrition of some bacterial growths, according to the strict aseptic manipulation program bacterium is cultivated, observes and measures in the aseptic experiment chamber then.Exist in the above-mentioned background technology trace routine loaded down with trivial details, can not direct viewing and calculate the shortcoming of measurement result.
Summary of the invention
The object of the present invention is to provide a kind of bacterium colony situation that is easy to observe, avoid the substratum dehydration simultaneously, influence bacteria culture medium of measurement result and preparation method thereof.
General idea of the present invention is:
It is a basic medium with the nutrient agar, also is added with water-holding agent and staining agent in the basic medium, and wherein, nutrient agar medium: water: water-holding agent: the weight ratio of staining agent is 36-40: 1000: 0.1-5: 0.01-0.1.
The preparation method of bacteria culture medium comprises following step:
A, earlier with nutrient agar medium: water: water-holding agent: staining agent is by 36-40: 1000: 0.1-5: after the weight ratio of 0.01-0.1 is weighed, placed apart;
B, load weighted nutrient agar medium and water are mixed with nutrient agar;
Add load weighted water-holding agent in c, the nutrient agar that in above-mentioned steps, prepares;
D, the substratum that contains water-holding agent for preparing in the c step is sterilized;
After the staining agent sterilization of e, weighing, add in the substratum of d step preparation, promptly make bacteria culture medium.
Other designs of the present invention are:
Nutrient agar medium in the bacteria culture medium: water: water-holding agent: the weight ratio of staining agent is 36-40: 1000: 0.1-1: 0.01-0.1.
Water-holding agent can select for use starch graft copolymer vinylformic acid (salt) super strength water absorbent, modified-cellulose class High hydrophilous resin, acryloyl derivative and inorganics etc. to be combined into High hydrophilous resin, staining agent can select for use the PHENOL 99.8 MIN ((CARBOLIC ACID)) azaleine, Congo red, the PHENOL 99.8 MIN ((CARBOLIC ACID)) sargassun is red, Viola crystallina, methyl orchid, TCC.
Preparation is during bacteria culture medium, and with nutrient agar medium: water: water-holding agent: staining agent is pressed 36-40: 1000: 0.1-1: the weight ratio weighing of 0.01-0.1.
Nutrient agar medium is mixed placement 8-12 minute with water, heat then, nutrient agar medium is fully dissolved.
When adding water-holding agent, elder generation adds in the nutrient agar after water-holding agent is ground into 80-100 purpose particle again, stirs 8-12 minute.
When containing the medium sterilization of water-holding agent, adopted autoclaving 12-18 minute, pressure, temperature 115-230 ℃.
When adding staining agent in the substratum that contains water-holding agent, elder generation sterilizes after staining agent is mixed with 5% aqueous solution, adds the nutrient agar that contains water-holding agent again.
The technical progress that the present invention obtains is:
Water-holding agent in the bacteria culture medium can be kept the moisture of substratum, avoids the dry accuracy that influences measurement result of culturing gene dehydration; Staining agent can be dyed redness or pink with most of bacterium colony, is convenient to observe detected result; Its preparation method's technological design is reasonable, simple to operation simultaneously.
Embodiment
Below embodiments of the invention are further described:
In the present embodiment, bacteria culture medium is a basic medium with nutrient agar (GB4789.28-1994), also be added with water-holding agent and staining agent in the basic medium, wherein, nutrient agar medium: water: water-holding agent: the weight ratio of staining agent is 38: 1000: 3: 0.01.
Preparation is during bacteria culture medium, should be according to the following steps:
A, preparation nutrient agar;
B, with the nutrient agar for preparing: water: water-holding agent: staining agent was by 38: 1000: 3: after 0.01 weight ratio is weighed, placed apart;
C, load weighted nutrient agar and water are mixed, places after 10 minutes, boil, make fully and dissolve every the asbestos wire net low baking temperature;
D, water-holding agent is ground into 100 purpose particles after, add in the nutrient agar for preparing in the above-mentioned steps, stirred 10 minutes;
E, the substratum that contains water-holding agent to preparing in the d step adopted autoclaving 15 minutes, pressure 1.385 * 10
5Pa, 125 ℃ of temperature.
F, sterilize after load weighted staining agent is mixed with 5% aqueous solution, add in the substratum that contains water-holding agent for preparing in the e step, promptly obtain bacteria culture medium.
The modified-cellulose class High hydrophilous resin that water-holding agent can select for use Beijing Institute of Technology to produce, staining agent can be selected the PHENOL 99.8 MIN ((CARBOLIC ACID)) azaleine for use.
The prepared bacteria culture medium of the present invention can place check-out console, plane culture dish or other culture medium carrier.
Claims (9)
1, a kind of bacteria culture medium, it is basic medium with the nutrient agar, it is characterized in that also being added with in the basic medium water-holding agent and staining agent, wherein, nutrient agar medium: water: water-holding agent: the weight ratio of staining agent is 36-40: 1000: 0.1-5: 0.01-0.1.
2, bacteria culture medium according to claim 1 is characterized in that nutrient agar medium: water: water-holding agent: the weight ratio of staining agent is 36-40: 1000: 0.01-1: 0.01-0.1.
3, bacteria culture medium according to claim 1 and 2, it is characterized in that water-holding agent can select for use starch graft copolymer vinylformic acid (salt) super strength water absorbent, modified-cellulose class High hydrophilous resin, acryloyl derivative and inorganics etc. to be combined into High hydrophilous resin, staining agent can select for use the PHENOL 99.8 MIN ((CARBOLIC ACID)) azaleine, Congo red, the PHENOL 99.8 MIN ((CARBOLIC ACID)) sargassun is red, Viola crystallina, methyl orchid, TCC.
4, the preparation method of bacteria culture medium according to claim 1 is characterized in that it comprises following step:
A, earlier with nutrient agar medium: water: water-holding agent: staining agent is by 36-40: 1000: 0.1-5: after the weight ratio of 0.01-0.1 is weighed, placed apart;
B, load weighted nutrient agar medium and water are mixed with nutrient agar;
Add load weighted water-holding agent in c, the nutrient agar that in above-mentioned steps, prepares;
D, the substratum that contains water-holding agent for preparing in the c step is sterilized;
After the staining agent sterilization of e, weighing, add in the substratum of d step preparation, promptly make bacteria culture medium.
5, the preparation method of bacteria culture medium according to claim 4, it is characterized in that nutrient agar medium: water: water-holding agent: staining agent is pressed 36-40: 1000: 0.1-1: the weight ratio weighing of 0.01-0.1.
6, the preparation method of bacteria culture medium according to claim 4 is characterized in that earlier nutrient agar medium being mixed with water placing 8-12 minute, heats then, and nutrient agar medium is fully dissolved.
7, the preparation method of bacteria culture medium according to claim 4, when it is characterized in that adding water-holding agent, elder generation adds in the nutrient agar after water-holding agent is ground into 80-100 purpose particle again, stirs 8-12 minute.
8, the preparation method of bacteria culture medium according to claim 4 is characterized in that when containing the medium sterilization of water-holding agent, adopts autoclaving 12-18 minute, pressure, temperature 115-230 ℃.
9, the preparation method of bacteria culture medium according to claim 4, when it is characterized in that adding staining agent in the substratum that contains water-holding agent, elder generation sterilizes after staining agent is mixed with 5% aqueous solution, adds the nutrient agar that contains water-holding agent again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410012136 CN1234848C (en) | 2004-01-29 | 2004-01-29 | Bacterium culture medium and preparation method therefor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410012136 CN1234848C (en) | 2004-01-29 | 2004-01-29 | Bacterium culture medium and preparation method therefor |
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| Publication Number | Publication Date |
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| CN1557943A true CN1557943A (en) | 2004-12-29 |
| CN1234848C CN1234848C (en) | 2006-01-04 |
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| CN 200410012136 Expired - Fee Related CN1234848C (en) | 2004-01-29 | 2004-01-29 | Bacterium culture medium and preparation method therefor |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102492765A (en) * | 2011-12-13 | 2012-06-13 | 江门市凯林贸易有限公司 | Agar culture medium and preparation method thereof |
| CN103091145A (en) * | 2013-01-30 | 2013-05-08 | 中国人民解放军军事医学科学院生物工程研究所 | Method for staining microorganisms |
| JP2023521428A (en) * | 2020-04-15 | 2023-05-24 | ラピッド マイクロ バイオシステムズ インコーポレイテッド | Background-attenuated microbiological nutrient medium and method of use thereof |
-
2004
- 2004-01-29 CN CN 200410012136 patent/CN1234848C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102492765A (en) * | 2011-12-13 | 2012-06-13 | 江门市凯林贸易有限公司 | Agar culture medium and preparation method thereof |
| CN103091145A (en) * | 2013-01-30 | 2013-05-08 | 中国人民解放军军事医学科学院生物工程研究所 | Method for staining microorganisms |
| CN103091145B (en) * | 2013-01-30 | 2015-04-29 | 中国人民解放军军事医学科学院生物工程研究所 | Method for staining microorganisms |
| JP2023521428A (en) * | 2020-04-15 | 2023-05-24 | ラピッド マイクロ バイオシステムズ インコーポレイテッド | Background-attenuated microbiological nutrient medium and method of use thereof |
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| Publication number | Publication date |
|---|---|
| CN1234848C (en) | 2006-01-04 |
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