CN1557826A - Method for extracting astragaloside and polysaccharide from traditional Chinese medicine astragalus and its capsule preparation - Google Patents
Method for extracting astragaloside and polysaccharide from traditional Chinese medicine astragalus and its capsule preparation Download PDFInfo
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- CN1557826A CN1557826A CNA2004100139504A CN200410013950A CN1557826A CN 1557826 A CN1557826 A CN 1557826A CN A2004100139504 A CNA2004100139504 A CN A2004100139504A CN 200410013950 A CN200410013950 A CN 200410013950A CN 1557826 A CN1557826 A CN 1557826A
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Abstract
The present invention relates to the preparation process and capsule preparation of astragaloside an and astragalus polysaccharide. Medicine material astragalus root is processed through water decoction, alcohol precipitation to obtain supernatant, concentration, butyl alcohol or sodium hydroxide solution extraction of the concentrate and decompression recovering to obtain astragaloside A; the alcohol precipitate is dried, water dissolved and alcohol precipitated repeatedly or ethanol washing, and drying to obtain astragalus polysaccharide. The astragaloside an and the astragalus polysaccharide are compounded with or without supplementary material, and the mixture is prepared into capsule. The capsule has the functions of benefiting vital energy, enhancing body resistance and nourishing lung and spleen and is used mainly in treating chronic bronchitis and obstructive pneumonectasis.
Description
Technical field:
The present invention relates to a kind of extracting method and capsule preparations thereof that from Chinese medicine astragalus, extracts Cyclosiversioside F and polysaccharide.
Background technology:
The Radix Astragali single preparation of China's listing has Radix Astragali essenc (" (Chinese traditional patent formulation preparation) " the 16, WS
3-B-3103-98) and HUANGQI KELI (" (Chinese traditional patent formulation preparation " the 11, WS
3-B-2248-96), all adopted simple percolation to extract Cyclosiversioside F, and this method, promptly poach, alcohol precipitation add extraction.Make like this and have the active polysaccharide component of important biomolecule and can not get keeping.Modern study shows: astragalus polysaccharides shows that to the experiment of stress reaction to have anti-fatigue anoxia-tolerance low temperature resistant, high temperature resistant, radiation resistance, the experiment of immune pharmacological action shown significantly improve the animal spleen weight, significantly strengthen macrophage phagocytosis of mice, promote serum haemolysis to form, improve plaque-forming bacteria's haematolysis ability and significantly increase the carbon clearance effect, more than effect not only exists under the normal physiological state, when body's immunity is low, show tangible immuno-potentiation equally simultaneously.If will discard polysaccharide put in the product and go, make Radix Astragali extract and capsule more can embody the effect of crude drug, curative effect must be better than Radix Astragali essenc, will obtain bigger social benefit and economic benefit.
Summary of the invention:
The present invention be directed to defective and deficiency that above-mentioned prior art exists, provide a kind of and can from Chinese medicine astragalus, extract Cyclosiversioside F and polysaccharide, extract and proper auxiliary materials proportioning, the method for composing prescription preparation, and prepare capsule with this method.
A kind of method of extracting Cyclosiversioside F and polysaccharide from Chinese medicine astragalus of the present invention may further comprise the steps:
Step 1: medicinal material decocts, and promptly Chinese medicine astragalus medicinal material or medicine materical crude slice get decoction liquor after decocting boils;
Step 2: carry out alcohol precipitation;
Step 3: with the supernatant liquor behind the alcohol precipitation, behind the recovery ethanol, concentrated solution gets carbon tetrol extraction liquid with the extraction of carbon tetrol, gets the sodium hydroxide solution extraction again, still gets carbon tetrol extraction liquid, reclaim under reduced pressure carbon tetrol, and concentrate drying gets the Cyclosiversioside F extract;
Step 4: with the throw out behind the alcohol precipitation, drying, dry thing is after repeatedly washing alcohol precipitation, and the taking precipitate drying promptly gets astragalus polysaccharide extract again.
Wherein decocting number of times in the step 1 is 3 times, and each amount of water is 6～10 times; Containing the alcohol amount in the step 2 during alcohol precipitation is 60～80%; What adopt in the step 3 is the sec-butyl alcohol extraction process, and volume ratio is 1～2: 1 during extraction, and number of times is 2～3 times, and when adopting the sodium hydroxide solution extraction, concentration of sodium hydroxide solution is 0.5～2%, and volume ratio is 1～2: 1, and number of times is 2～3 times.
The present invention mixes the Cyclosiversioside F extract with polyoses extract, add or do not add the capsule that auxiliary material is made.
The operational path that the present invention adopts water extraction and alcohol precipitation method to extract extracts Cyclosiversioside F and polysaccharide as much as possible from medicinal material.
The purification process of Cyclosiversioside F adopts the sec-butyl alcohol method of extraction among the present invention.
The purification process of polysaccharide adopts the alcohol precipitation to be dissolved in water among the present invention, and the method for repeated treatments is carried out, not only can be with tannin, and protein is all removed, and its purity is improved.
The present invention has overcome the defective and the deficiency of present Radix Astragali essenc, with Cyclosiversioside F and the polysaccharide that the present invention obtains, and active high, stable performance, has beneficial gas-solid originally, take a tonic or nourishing food to build up one's health the effect of lung spleen, be mainly used in treatment chronic bronchitis (catabasis), the obstructive emphysema syndrome of deficiency of QI.
The present invention utilizes this extracting method to obtain Cyclosiversioside F and polysaccharide fraction, makes capsule.The maximum tolerated dose that mouse is once irritated capsule for treating gastropathy is crude drug in whole 357g/kg, is about 793 times of clinical dosage crude drug in whole 0.45g/kg/ day.
Embodiment:
Embodiment one extracts each component for the present invention from Chinese medicine astragalus: get Radix Astragali medicine materical crude slice 6.7kg, add aquae destillata and decoct 3 times, for the first time with 10 times of amount decoctions 2 hours, the 2nd time, decocted 1 hour with 8 times of amounts and 6 times amount respectively, merge decoction liquor for 3 times, filter, filtrate is concentrated into every 1ml and is equivalent to crude drug 2g, uses ethanol sedimentation, makes amount of alcohol reach 75%, refrigeration is spent the night, filter, divide and get filtrate I and precipitation I two portions, standby.Filtrate I reclaims ethanol and is concentrated into relative density 1.3～1.4 (20 ℃), adding ethanol fully stirs evenly, make ethanol content reach 85%, refrigeration is spent the night, filter, filtrate recycling ethanol also is concentrated into every 1ml and is equivalent to crude drug in whole 10g, extract 3 times by 2: 1 volume ratios with water saturated sec-butyl alcohol, merge sec-butyl alcohol, with 1: 1 by volume extraction treatment of 1%NaoH solution 2 times, collect and the reclaim under reduced pressure sec-butyl alcohol, when being concentrated into relative density 1.3～1.4 (20 ℃), in 70～80 ℃ of vacuum-dryings, get the Cyclosiversioside F extract; I is 70～80 ℃ of following vacuum-dryings for precipitation, and with 1: 50 times distilled water heated and boiled dissolving, refrigeration is spent the night then, get supernatant liquor, filter, filtrate is concentrated into every 1ml and is equivalent to crude drug in whole 5g, adds ethanol sedimentation, make and contain alcohol amount in the solution and reach 80%, leave standstill clarification, the leaching precipitation, precipitation is used absolute ethanol washing respectively 2 times, in 70～80 ℃ of following vacuum-dryings, get astragalus polysaccharide extract.
Embodiment two extracts each component for another kind of the present invention from Chinese medicine astragalus: get Radix Astragali medicine materical crude slice 6.7kg, and boiling 3 times, 10 times of amounts of the 1st usefulness decocted 2 hours, the 2nd time, 3 times all with 8 times of amount decoctions 1.5 hours, merging decoction liquor, filter, filtrate is concentrated into every 1ml and is equivalent to crude drug 1g, with the second precipitation, makes amount of alcohol reach 70%, refrigeration is spent the night, filter, divide and get filtrate I and precipitation I two portions, standby.Filtrate I reclaims ethanol and concentrates relative density 1.2～1.3 (20 ℃), extract 3 times by 1.5: 1 volume ratios with water saturated sec-butyl alcohol, merge sec-butyl alcohol, with 2: 1 by volume extraction treatment of 1%NaoH solution 2 times, collect sec-butyl alcohol, when being concentrated into relative density 1.2～1.3 (20 ℃),, get the Cyclosiversioside F extract in 70～80 ℃ of following vacuum-dryings; Precipitation I adds 1: 40 times of distilled water heated and boiled dissolving, and refrigeration is spent the night, and gets supernatant liquor, filters, filtrate is concentrated into every 1ml and is equivalent to crude drug in whole 4g, adds ethanol sedimentation, makes to contain alcohol amount in the solution and reach 85%, leaves standstill clarification, the leaching precipitation in 70～80 ℃ of following vacuum-dryings, gets astragalus polysaccharide extract.
Embodiment three is tragacanth capsule preparation preparation method: get above-mentioned Cyclosiversioside F and astragalus polysaccharide extract, after mixing, add micropowder silica gel-calcium sulfate (25: 7) in right amount, make 1000, every capsules 0.25g.
Embodiment four is tragacanth capsule preparation preparation method: get above-mentioned Cyclosiversioside F and astragalus polysaccharide extract, after mixing, add microcrystalline cellulose-starch (2: 1) in right amount, make 1000 of capsules, every capsules 0.25g.
Embodiment five Radix Astragali extracts are used for the treatment of the animal experiment of chronic bronchitis
Method: choose 60 Kunming mouses, male, body weight 18～20g is divided into 6 groups at random by body weight, every group 10, except that the normal control group, all the other each treated animals are all used the ammoniacal liquor of 0.3ml/2.4L air, with per 15～20min repetitive stimulation 1 time, each 2～3min, stimulated 3 days every day 8 times, and the chronic bronchitis shape appears in animal after 30 days.Press each component difference gastric infusion of table 11 time/day, continuous 15 days, claim the weight of animals (g) time morning after the last administration, femoral artery sacrificed by exsanguination animal opens chest and takes out lungs then, claims full lung heavy (g), calculates each mouse lung exponential sum administration group inhibiting rate (%).
Watch 1: Radix Astragali extract is to slow segmental bronchus model mice lung exponential influence due to the ammoniacal liquor
| Group | Mouse number (only) | Dosage * fate (mg/kg * d) | The lung index (the g/100g body weight, X ± SD) | Inhibiting rate (%) |
| The normal control group | ???10 | ??????-- | ????0.849±0.147 | ?????-- |
| Slowly prop up scorching model group | ???10 | ??????-- | ????1.323±0.245 | ?????-- |
| Dexamethasone group | ???10 | ????1mg×15 | ????0.920±0.137 | ????30.45 |
| Radix Astragali extract | ???10 | ????300×15 | ????0.941±0.187 | ????28.85 |
| Radix Astragali extract | ???10 | ????150×15 | ????1.033±0.183 | ????21.95 |
| Radix Astragali extract | ???10 | ????75×15 | ????1.066±0.245 | ????19.46 |
The result shows: scorching type lung index (P＜0.001, P＜0.01, P＜0.05) is propped up in the extremely remarkable or obvious respectively inhibition of 300～700mg/kg Radix Astragali extract slowly.
Claims (7)
1, a kind of method of from Chinese medicine astragalus, extracting Cyclosiversioside F and polysaccharide, this method may further comprise the steps:
Step 1: medicinal material decocts, and promptly Chinese medicine astragalus medicinal material or medicine materical crude slice get decoction liquor after decocting boils;
Step 2: carry out alcohol precipitation;
Step 3: with the supernatant liquor behind the alcohol precipitation, behind the recovery alcohol, concentrated solution gets the sec-butyl alcohol extraction liquid with the extraction of carbon tetrol, gets the sodium hydroxide solution extraction again, still gets carbon tetrol extraction liquid, and behind the reclaim under reduced pressure carbon tetrol, concentrate drying gets the Cyclosiversioside F extract;
Step 4: with the throw out behind the alcohol precipitation, drying, dry thing is after repeatedly washing alcohol precipitation, and the taking precipitate drying promptly gets astragalus polysaccharide extract again.
2, extracting method according to claim 1 is characterized in that decocting time is 3 times in this method steps one, and each amount of water is 6～10 times.
3, extracting method according to claim 1 is characterized in that carrying out alcohol precipitation with ethanol, extracts with sec-butyl alcohol.
4, extracting method according to claim 1, requiring when it is characterized in that in this method steps two alcohol precipitation to contain the alcohol amount is 60～80%.
5, extracting method according to claim 1 is characterized in that adopting in the step 3 in this method the sec-butyl alcohol extraction process.Volume ratio is that 1～2: 1 extraction times is 2～3 times.When adopting the sodium hydroxide solution extraction simultaneously, concentration of sodium hydroxide solution is 0.5%～2%, and the extraction volume ratio is 1～2: 1, and extraction times is 2～3 times.
6, extracting method according to claim 1, containing the alcohol amount when it is characterized in that in this method in the step 4 alcohol precipitation is 70～90%.
7, the capsule preparations of a kind of Cyclosiversioside F and polysaccharide, it is characterized in that the two mixture of Cyclosiversioside F and astragalus polysaccharide extract after, add or do not add the capsule that auxiliary material is made.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2004100139504A CN1557826A (en) | 2004-01-19 | 2004-01-19 | Method for extracting astragaloside and polysaccharide from traditional Chinese medicine astragalus and its capsule preparation |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100500686C (en) * | 2006-05-12 | 2009-06-17 | 神威药业有限公司 | Method for extraction and preparation of astragaloside |
| CN101088521B (en) * | 2006-06-14 | 2010-10-27 | 北京四环科宝制药有限公司 | Astragalus medicine prepn and its prepn process |
| CN102093456A (en) * | 2011-02-23 | 2011-06-15 | 南京工业大学 | Method for extracting astragaloside IV from astragalus |
| CN101619107B (en) * | 2009-08-17 | 2011-06-29 | 杭州科皇饲料有限公司 | Astragalus polysaccharide extraction method |
| CN1985875B (en) * | 2006-12-14 | 2011-07-20 | 天津科技大学 | Preparing process of Jade-screen total polyose |
| CN1854158B (en) * | 2005-04-25 | 2011-09-07 | 蒋来高 | Extraction of astragalus mongholicus polysaccharose by super-critical fluid technology |
| CN102443072A (en) * | 2010-10-12 | 2012-05-09 | 沈阳药科大学 | Method for simultaneously extracting polysaccharides and saponins from astragalus |
| CN102641326A (en) * | 2012-05-17 | 2012-08-22 | 中南大学 | Membranous milkvetch root extract, as well as preparation and application methods thereof |
| CN107582585A (en) * | 2017-11-08 | 2018-01-16 | 河南中医药大学 | Treat traditional Chinese medicinal components side and its application of COPD syndrome of qi deficiency of lung and kidney |
| CN109846922A (en) * | 2017-11-30 | 2019-06-07 | 贵州益佰制药股份有限公司 | Application of the Aidi preparation in preparation treatment chronic obstructive emphysema drug |
| EP4122480A4 (en) * | 2020-03-17 | 2024-04-24 | PhytoHealth Corporation | Use of astragalus medicinal composition for preparing drug for enhancing cancer therapy |
-
2004
- 2004-01-19 CN CNA2004100139504A patent/CN1557826A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1854158B (en) * | 2005-04-25 | 2011-09-07 | 蒋来高 | Extraction of astragalus mongholicus polysaccharose by super-critical fluid technology |
| CN100500686C (en) * | 2006-05-12 | 2009-06-17 | 神威药业有限公司 | Method for extraction and preparation of astragaloside |
| CN101088521B (en) * | 2006-06-14 | 2010-10-27 | 北京四环科宝制药有限公司 | Astragalus medicine prepn and its prepn process |
| CN1985875B (en) * | 2006-12-14 | 2011-07-20 | 天津科技大学 | Preparing process of Jade-screen total polyose |
| CN101619107B (en) * | 2009-08-17 | 2011-06-29 | 杭州科皇饲料有限公司 | Astragalus polysaccharide extraction method |
| CN102443072A (en) * | 2010-10-12 | 2012-05-09 | 沈阳药科大学 | Method for simultaneously extracting polysaccharides and saponins from astragalus |
| CN102093456A (en) * | 2011-02-23 | 2011-06-15 | 南京工业大学 | Method for extracting astragaloside IV from astragalus |
| CN102093456B (en) * | 2011-02-23 | 2013-11-06 | 南京工业大学 | Method for extracting astragaloside IV from astragalus |
| CN102641326A (en) * | 2012-05-17 | 2012-08-22 | 中南大学 | Membranous milkvetch root extract, as well as preparation and application methods thereof |
| CN102641326B (en) * | 2012-05-17 | 2013-12-18 | 中南大学 | Astragalus extract, as well as preparation and application methods thereof |
| CN107582585A (en) * | 2017-11-08 | 2018-01-16 | 河南中医药大学 | Treat traditional Chinese medicinal components side and its application of COPD syndrome of qi deficiency of lung and kidney |
| CN107582585B (en) * | 2017-11-08 | 2020-08-25 | 河南中医药大学 | Traditional Chinese medicine component formula for treating lung-kidney qi deficiency syndrome of chronic obstructive pulmonary disease and application thereof |
| CN109846922A (en) * | 2017-11-30 | 2019-06-07 | 贵州益佰制药股份有限公司 | Application of the Aidi preparation in preparation treatment chronic obstructive emphysema drug |
| EP4122480A4 (en) * | 2020-03-17 | 2024-04-24 | PhytoHealth Corporation | Use of astragalus medicinal composition for preparing drug for enhancing cancer therapy |
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