CN1528681A - Live bacterial microorganism water modifier equipped with activating culture medium and manufacturing process thereof - Google Patents
Live bacterial microorganism water modifier equipped with activating culture medium and manufacturing process thereof Download PDFInfo
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- CN1528681A CN1528681A CNA031583652A CN03158365A CN1528681A CN 1528681 A CN1528681 A CN 1528681A CN A031583652 A CNA031583652 A CN A031583652A CN 03158365 A CN03158365 A CN 03158365A CN 1528681 A CN1528681 A CN 1528681A
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Abstract
The invention relates to a living bacterial microbe water modifying agent assorted with activating culture medium and its making method, used to regulate and control and modify the water quality of the culture of aquatic product and fancy fishes. It mainly adopts the culture medium carbon or nitrogen source matter, adds in water and places in fermentation cylinder for high-temperature sterilization and cooling, adds in living bacterial microbe to inoculate and ferment, adds in carrier and places in the drier to dry, so as to obtain the semi-finished product solid microbe, which is held together with the semi-finished product activating culture medium made by direct split holding, so as to obtain the finished product. It can convert and decompose the organic substance in the aerobic layer of the water body, converting carbonaceous organic substance into carbon dioxide and water, and aminating nitrogenous organic substance to form ammonia nitrogen, then which is converted into nitrate and nitrite, and finally converting the ammonia nitrogen into ammonia to escape from the water and at first the ammonia activates the living bacterial microbe and then the living bacterial microbe activated quickly breeds, thus able to quickly modify the water body and the using effect is fine.
Description
Technical field
The present invention relates to a kind of modify-water agent of viable bacteria microorganism and manufacturing process thereof of being furnished with activation medium, specifically the biological modify-water agent of this solid live bacteria with the activation of activation medium under, the short period of time activation also reaches high-density, is used for regulating and controlling water quality and improvement that aquatic products and fancy fishes are cultured.
Background technology
Before the present invention made, the biological modify-water agent of existing viable bacteria normally utilized some to have organic amino and ammonia nitrogen function in the water of decomposition and the genus bacillus that purifies water, by fermenting under certain condition preparing with the density of breeding its genus bacillus.The fermentation process of this fermentation propagation genus bacillus normally adds a large amount of yeast extract medicine and peptone direct fermentation in initial fermented liquid.Also having a kind of method in addition is on the basis of this method, after inoculation fermentation begins, slowly adds the iron content nutritive medium constantly in fermentor tank, adds simultaneously carbon source and nitrogenous source in right amount, reaches requirement to the OD value of fermented liquid and stops to ferment.Adopt the microbial water-purifying agent of these method manufacturings to be liquid product, microbe population is few in transportation inconvenience and the unit volume, and small product size is big.But the simple solid microbe water purification agent that overcomes above shortcoming and produce is because microorganism becomes dormant state after curing, cause microorganism in use can not activate breeding rapidly, so onset time is long after using, poor effect, and single genus bacillus can not be satisfied the demand of decomposing all objectionable impuritiess in the water quality improvement process immediately.Though genus bacillus can be converted into products such as nitrate with the ammonia nitrogen in the organism in the water, but still is present in the water, does not play the effect of decomposition completely.
Summary of the invention
The objective of the invention is to overcome above-mentioned weak point, thereby after providing one or more microorganisms to carry out high density fermentation, under the oven dry effect of dryer or Vacuumdrier, be cured as the little solid microbe water purification agent of high-density volume, make the solid microbe water purification agent be convenient to transportation, store; Owing to the effect of join activation medium, in use can make the microorganism activation and the breeding rapidly of dormancy in solid state rapidly; Because the interaction of microorganism is the objectionable impurities in the water of decomposition and make it become the required nutritive ingredient of self cell proliferation and rapid absorption effectively, thereby fundamentally reaches a kind of modify-water agent of viable bacteria microorganism and manufacturing process thereof of being furnished with activation medium of water purification purpose.
Main solution of the present invention is achieved in that
The present invention is furnished with its composition proportioning percentage ratio calculating by weight of viable bacteria microorganism modify-water agent of activation medium.
One. the first group: solid microbe adopts following component processing:
1. 1000 grams, substratum: 2-200g/l fetch water;
2. viable bacteria microbial inoculant amount is 1-50%;
3. carrier add-on and liquid microbe bacterium liquid are 10-90 to making a gesture of measuring.
Two. the first group: solid microbe content is: 10-99.9;
Three. the second group: activation medium adopts carbon source material or nitrogen source content to be: 0.1-90
Production technique of the present invention is as follows:
One. first group: the half-finished production technique of solid microbe:
1. get: water, substratum, substratum are that carbon source material or nitrogen source are put into fermentor tank through high-temperature sterilization, and sterilization time is: 10-240 minute, temperature was: 60-150 ℃;
2. cool off: be cooling time: 6-24 hour, add the inoculation of viable bacteria microbial strains after temperature is reduced to 5-45 ℃;
3. ferment: fermentation time is: 0-120 hour, measuring the effective bacterium quantity of microorganism was 10
3Individual/ml-10
25Individual/as during ml, to stop fermentation, take out fermented liquid;
4. add carrier and mix in above-mentioned fermented liquid, be positioned over interior oven dry of drying machine then and be that solid, time of drying are: 5 minutes-10 hours, bake out temperature was: 20-120 ℃.
Two. the second group is got the activation medium direct packaging and is become work in-process;
Three. first group solid microbe work in-process and second group activation medium work in-process are packed two inclusions respectively be fitted together and be finished product.
Viable bacteria microbial strains of the present invention is the dead leaf genus bacillus, Bacillus licheniformis, Bacilluscereus,, bacillus natto, lactobacillus, streptococcus faecalis, streptococcus faecium, Candida utilis, cereuisiae fermentum, candida tropicalis, Rhodopseudomonas palustris, Rhodopseudomonas spheroides, capsula Rhodopseudomonas, bacterium nitrobacter, the nitrosification bacillus, Nocardia bacteria, black aspergillus, aspergillus, lactobacterium casei, plant lactobacillus, pediococcus acidilactici, Lactobacterium acidophilum, streptococcus uberis.(parent of above all bacterial classifications provides by institute of microbiology of the Chinese Academy of Sciences (Beijing) China common micro-organisms kind storehouse).Carrier of the present invention is: inferior powder, starch, flour, wheat bran, zeolite powder, lactose, lime carbonate, diatomite, cyclodextrin, dextrin.
Activation culture based raw material of the present invention is a carbon source material as organic carbohydrate, alcohols, organic acid, hydro carbons, protein and hydrolyzate thereof.Specifically be glucose, fructose, maltose, sucrose, molasses, seminose, dextran, Nutriflora P, lactose and the starch in organic carbohydrate.Ethanol in the alcohols and the formic acid in the acids, acetate, pyruvic acid, oxysuccinic acid, α-Tong Wuersuan, succsinic acid and fumaric acid.Methane in the hydro carbons, ethane, propane, butane; Or nitrogen source is as ammonium salt and nitrate, urea, peptone, yeast leach liquor, Semen Maydis powder, soyflour, soybean cake powder, protein (fish meal, digested tankage, bone meal etc.) and amino acid.
Compared with the prior art the present invention has the following advantages: the present invention in use earlier puts into the modify-water agent of solid live bacteria microorganism and activation medium and pond water or water container and activates 5 minutes-10 hours and pouring into then; Because the reactivation process that this product has passed through certain hour, can rapid aerobic layer organic substance be transformed decomposition after entering water body environment at water body, carbonaceous organism is converted into carbonic acid gas and water, and with the itrogenous organic substance ammonification, form ammonia nitrogen and then ammonia nitrogen is converted into nitrate and nitrite, finally ammonia nitrogen is converted into that ammonia overflows and result of use is good from water; Manufacturing process is simple, and is easy to make; Invest for a short time, adopt conventional equipment, and packing rationally, transportation, storage are easy to use, have effectively overcome deficiency of the prior art.
Embodiment:
Below the present invention is further described in conjunction with the embodiments:
Embodiment one:
First group solid microbe work in-process water intakings: 1000 grams, get yeast leach liquor 5 grams in the nitrogen source in the substratum, be placed in the fermentor tank through high-temperature sterilization, sterilization time is: 120 minutes, temperature was: 80 ℃; Cooling cooling was then cooled off 5 hours, when temperature is cooled to 22 ℃, added the inoculation of viable bacteria microbial strains.It is 10 milliliters that microbial strains is got the dead leaf genus bacillus, is inoculated in and places the above-mentioned fermented liquid of fermentor tank to ferment, and reaches 10 when detecting the effective quantity of microorganism
8Individual/as its fermented liquid to be taken out from fermentor tank during ml and add carrier, carrier is got time powder 250 grams and is mixed, and again will its mixture places in dryer with 100 ℃ temperature oven dry 1 hour, and taking out packing then becomes solid microbe water purification agent semi-finished product for standby.The second group gets that sucrose 1% (weight percentage) packing becomes the activation medium work in-process in the activation medium.First group solid microbe water purification agent work in-process are got 99% (weight percentage), and second group activation medium work in-process are got 1% (weight percentage) and are packaged as two inclusions respectively and become finished product.Two bags are loaded in the container together, test in Jintan, Changzhou.
Test method is as follows:
Get solid microbe modify-water agent 3000g, brown sugar 500g, activation medium 1g,
Detect test paper and instrument: the U.S. produces 3M total plate count rapid determination test paper, water quality quick analytic instrument.Test method: get 30 kilograms in the pool cultivated water of shrimp, on average be placed in three plastic tanks 10 kilograms every barrel.In 3 buckets, put into each 1000g of microorganism modify-water agent respectively.One of them bucket as the blank group, is put into 500g brown sugar for one in other two buckets, and another adds the 1g activation medium.Test the total plate count of water quality in 3 buckets immediately.Placed 4 hours at natural temperature (30 ℃) then, measure the total plate count of water quality again.The test tank (basic condition of water quality is close) that after having surveyed 3 barrels of liquid is added 31 mu was respectively measured water quality after 24 hours.
Below in the present invention and the prior art: the mensuration and the water-quality determination of water quality microbial bacteria number:
The mensuration of table 1 water quality microbial bacteria number
Unit: individual/milliliter
| Prior art | The present invention | ||
| Project | 1kg microorganism modify-water agent+10Kg Chi Shui | 1kg microorganism modify-water agent+500g brown sugar+10kg Chi Shui | 1Kg microorganism modify-water agent+10Kg Chi Shui |
| 0h bacterium number | ???3×10 8 | ?3×10 8 | ??3×10 8 |
| 4h bacterium number | ???5×10 8 | ?1×10 9 | ??8×10 9 |
As shown in Table 1, the effect through 4 hours, the total plate count of control group has increased by 67%, and the test group that adds brown sugar has increased by 233%, and the total plate count that adds activation medium has then increased by 2567%.By statistics with check, the test-results significant difference of test group of the present invention and other two prior art groups (p＜0.01). showing that activation medium among the present invention has the viable count in the solid microbe product activates and promoter action.Water-quality determination the results are shown in Table 2.
Table 2 after 24 hours microorganism strains to the action effect of cultivation water
Unit: %
| Prior art | The present invention | |||
| Project | Control group | 1kg microorganism modify-water agent+10Kg Chi Shui | 1kg microorganism modify-water agent+500g brown sugar+10kg Chi Shui | 1Kg microorganism modify-water agent+10Kg Chi Shui |
| ????PH | ????0.0 | ???0.0 | ?+1.0 | ???+2.1 |
| ????DO | ????-8.5 | ???+2.2 | ?+7.5 | ???+10.2 |
| ????NH4+-N | ????+37.2 | ???-8.8 | ?-17.2 | ???-34.2 |
| ????NO2--N | ????+12 | ???-5.2 | ?-12.0 | ???-32.5 |
| ????S2- | ????+26.0 | ???-2.3 | ?-6.0 | ???-9.5 |
By table 2 as seen, use microorganism modify-water effect of the present invention obviously to strengthen, it can increase substantially dissolved oxygen content in the water body, quick degradation of ammonia nitrogen, and harmful water prime factors such as nitrite nitrogen and sulfide, thus safeguard the benign cycle of water ecological setting.Because of the modify-water effect of microniological proudcts mainly is based on the Physiology and biochemistry effect of microbial population, so the activation medium among the present invention mainly is to reach the effect of increasing water quality and purifying by the increase that promotes living microorganism quantity.
Embodiment two: first group solid microbe water purification agent work in-process are got 1 premium on currency, glucose 100 grams that substratum is got in the carbon source material are put into fermentor tank through high-temperature sterilization, temperature is 120 ℃, cooling then, cooled off 15 hours, when temperature is reduced to 25 ℃, add the viable bacteria microbial inoculant, get Bacillus licheniformis, lactobacillus is 250 milliliters, the production technique among the embodiment one is all adopted in its inoculation and fermentation, with existing Bacillus licheniformis, lactobacillus bacterium bacterial classification inoculation also places the initial fermented liquid of fermentor tank to ferment, when the effective quantity of microorganism of fermenting reaches 3 * 10
8Individual/as to stop fermentation during Ml, and it is taken out wheat bran 500 grams that add in the carrier mix from fermentor tank, again its mixture is positioned in the dryer and is distributed into solid microbe water purification agent work in-process after the temperature oven dry taking-up in 2.5 hours with 60C; Getting ammonia chloride and sulfate of ammoniac again, to become the activation medium work in-process with 1: 1 mixed packing be the second group.Getting first group solid microbe water purification agent work in-process is 90% (weight percentage), gets second group activation medium work in-process: 10% (weight percentage).Above-mentioned first group and second group are divided into two bags respectively.Attaching together becomes the viable bacteria microorganism modify-water agent finished product of being furnished with activation medium and tests in Jintan, Changzhou in the plastics bag.
Get microorganism modify-water agent 3000g, brown sugar 500g, activation medium 10g, detect test paper and instrument: the U.S. produces 3M total plate count rapid determination test paper, water quality quick analytic instrument.
Test method: get 30 kilograms in the pool cultivated water of shrimp, on average be placed in three plastic tanks 10 kilograms every barrel.In 3 buckets, put into each 1000g. of microorganism modify-water agent respectively.One of them bucket as the blank group, is put into 500g brown sugar for one in other two buckets, and another adds the 10g activation medium.Test the total plate count of water quality in 3 buckets immediately.Placed 4 hours at natural temperature (30 ℃) then, measure the total plate count of water quality again.The test tank (basic condition of water quality is close) that after having surveyed 3 barrels of liquid is added 31 mu was respectively measured water quality after 24 hours.Can reach a conclusion by detected result and to show that complex microorganism modify-water agent of the present invention is after cooperating activation medium to use, increase substantially dissolved oxygen content in the water body, quick degradation of ammonia nitrogen, harmful water such as nitrite nitrogen and sulfide prime factor, thus the benign cycle of water ecological setting safeguarded.Because of the modify-water effect of microniological proudcts mainly is based on the Physiology and biochemistry effect of microbial population, so the activation medium among the present invention mainly is to reach the effect of increasing water quality and purifying by the increase that promotes living microorganism quantity.
Embodiment three: first group solid microbe water purification agent work in-process are got 1 premium on currency, and substratum is got oxysuccinic acid 190 gram in the carbon source material and put into fermentor tank and carry out high-temperature sterilization, and temperature is 130 ℃, time is: 20 minutes, cool then, when temperature is reduced to 40 ℃, add the inoculation of viable bacteria microbial strains.Get withered Bacilluscereus,, bacillus natto, streptococcus faecalis, cereuisiae fermentum, Rhodopseudomonas palustris, Rhodopseudomonas spheroides, lactobacillus and be 500 milliliters altogether, production method among the embodiment one, two, inoculation, the effective quantity of microorganism reaches 3 * 10
11Individual/add during Ml diatomite in the carrier, zeolite powder totally 750 grams mix again its mixture to be positioned in the Vacuumdrier and take out packing after dry 2 hours under less than an atmospheric situation and become solid microbe water purification agent work in-process.Getting fish meal, bone meal, to be packed as the activation medium work in-process with 1: 1 mixed be the second group.Getting first group solid microbe water purification agent work in-process is 50 (weight percentage), getting second group activation medium work in-process is 50% (weight percentage), be distributed into two bags respectively and be packaged into again in the Plastic Bottle, become the viable bacteria microorganism modify-water agent finished product of being furnished with activation medium.
Get microorganism modify-water agent 3000g, brown sugar 500g, activation medium 1000g, detect test paper and instrument: the U.S. produces 3M total plate count rapid determination test paper, water quality quick analytic instrument.
Test method: get 30 kilograms in the pool cultivated water of shrimp, on average be placed in three plastic tanks 10 kilograms every barrel.In 3 buckets, put into each 1000g. of microorganism modify-water agent respectively.One of them bucket as the blank group, is put into 500g brown sugar for one in other two buckets, and another adds 1000g, activation medium.Test the total plate count of water quality in 3 buckets immediately.Placed 4 hours at natural temperature (30 ℃) then, measure the total plate count of water quality again.The test tank (basic condition of water quality is close) that after having surveyed 3 barrels of liquid is added 31 mu was respectively measured water quality after 24 hours.Can reach a conclusion by detected result and to show that complex microorganism modify-water agent of the present invention is after cooperating activation medium to use, increase substantially dissolved oxygen content in the water body, quick degradation of ammonia nitrogen, harmful water such as nitrite nitrogen and sulfide prime factor, thus the benign cycle of water ecological setting safeguarded.Because of the modify-water effect of microniological proudcts mainly is based on the Physiology and biochemistry effect of microbial population, so the activation medium among the present invention mainly is to reach the effect of increasing water quality and purifying by the increase that promotes living microorganism quantity.
In sum, the viable bacteria microorganism modify-water agent of being furnished with activation medium involved in the present invention can effectively improve number of viable and the surviving rate after the solid microbe product enters water body, thereby improves the usefulness of microniological proudcts modify-water.Activation medium is used in conjunction with microorganism modify-water agent product, can play fast, the result of use that synergy etc. are good really solves the existing problem of present microorganism modify-water agent.
Claims (8)
1. viable bacteria microorganism modify-water agent of being furnished with activation medium is characterized in that adopting following component to form, and component is pressed the proportioning weight percentage:
(1) solid microbe adopts following component processing:
A. 1000 grams, substratum: 2-200g/l fetch water;
B. viable bacteria microbial inoculant amount is 1-50%;
C. carrier add-on and liquid microbe bacterium liquid are 10-90 to making a gesture of measuring;
(2) solid microbe content is: 10-99.9;
(3) activation medium adopts carbon source material or nitrogen source content to be: 0.1-90.
2. viable bacteria microorganism modify-water agent of being furnished with activation medium according to claim 1, the bacterial classification that it is characterized in that described viable bacteria microorganism is the dead leaf genus bacillus, Bacillus licheniformis, Bacilluscereus,, bacillus natto, lactobacillus, streptococcus faecalis, streptococcus faecium, Candida utilis, cereuisiae fermentum, candida tropicalis, Rhodopseudomonas palustris, Rhodopseudomonas spheroides, capsula Rhodopseudomonas, bacterium nitrobacter, the nitrosification bacillus, Nocardia bacteria, black aspergillus, aspergillus, lactobacterium casei, plant lactobacillus, pediococcus acidilactici, Lactobacterium acidophilum, streptococcus uberis.
3. viable bacteria microorganism modify-water agent of being furnished with activation medium according to claim 1 is characterized in that the effective bacterium quantity of described viable bacteria microorganism is: 10
3Individual/mL-10
25Individual/mL
4. viable bacteria microorganism modify-water agent of being furnished with activation medium according to claim 1 is characterized in that described carrier is: inferior powder, starch, flour, wheat bran, zeolite powder, lactose, lime carbonate, diatomite, cyclodextrin, dextrin.
5. viable bacteria microorganism modify-water agent of being furnished with activation medium according to claim 1, it is characterized in that described carbon source material is organic carbohydrate, alcohols, organic acid, hydro carbons, protein and hydrolyzate thereof specifically are glucose, fructose, maltose, sucrose, molasses, seminose, dextran, Nutriflora P, lactose and the starch in organic carbohydrate.Ethanol in the alcohols and the formic acid in the acids, acetate, pyruvic acid, oxysuccinic acid, α-Tong Wuersuan, succsinic acid and fumaric acid.Methane in the hydro carbons, ethane, propane, butane;
6. viable bacteria microorganism modify-water agent of being furnished with activation medium according to claim 1 is characterized in that described nitrogen source is: ammonium salt and nitrate, urea, peptone, yeast leach liquor, Semen Maydis powder, soyflour, soybean cake powder, protein.
7, according to claim 1 or 6 described viable bacteria microorganism modify-water agent of being furnished with activation medium, it is characterized in that described protein is fish meal, digested tankage, bone meal.
8. produce the described production method of being furnished with the viable bacteria microorganism modify-water agent of activation medium of claim 1 for one kind, it is characterized in that:
(1) the half-finished production technique of solid microbe:
A, get: water, substratum, substratum are that carbon source material or nitrogen source are put into fermentor tank through high-temperature sterilization, and sterilization time is: 10-240 minute, temperature was: 60-150 ℃;
B.: be cooling time: 6-24 hour, add the inoculation of viable bacteria microbial strains after the temperature cooling is reduced to 5-45 ℃;
C, ferment: fermentation time is: 0-120 hour, measuring the effective bacterium quantity of microorganism was 10
3Individual/ml-10
25Individual/as during ml, to stop fermentation, take out fermented liquid;
D. add carrier in the fermentation and mix, be positioned over interior oven dry of drying machine then and be that solid, time of drying are: 5 minutes-10 hours, bake out temperature was: 20-120 ℃.
(2) get the activation medium direct packaging;
(3) solid microbe work in-process and activation medium work in-process being packed two inclusions respectively is fitted together and is finished product.
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