CN1528118A - Temilmyces fuliginosus large-scale liquid deep fermentation production process - Google Patents
Temilmyces fuliginosus large-scale liquid deep fermentation production process Download PDFInfo
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- CN1528118A CN1528118A CNA2003101060815A CN200310106081A CN1528118A CN 1528118 A CN1528118 A CN 1528118A CN A2003101060815 A CNA2003101060815 A CN A2003101060815A CN 200310106081 A CN200310106081 A CN 200310106081A CN 1528118 A CN1528118 A CN 1528118A
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Abstract
The present invention is aimed at providing a large-scale liquid deep bed fermentation process to produce termitophile mycelium. Said invention uses termitophile fungus from Jinxiang Inst. of Fungi, Shandong Prov. of China as initial strain, adopts slope to make liquid shave-flask cultivation, liquid shake-flask extension cultivation, first-stage seed cultivation and fermentation cultivation, finally, make the fermentation tank volume be up to 1000-5000 L. The utilization of said invented fermentation process makes fermentation in 1500L fermentation tank for 92 hr., the mycelium in the fermented liquor (by dried weight) can be up to 4.6%.
Description
Technical field
The present invention relates to a kind of chicken fir large-scale deep liquid fermentation production technology.
Technical background
Chicken fir formal name used at school Termitomyces albuminosus (Berk.) Heim. is the precious wild edible fungus of being born in a kind of summer and autumn on the termitarium fungus garden, belongs to (Amanitaceae) ant nest Agaricus of amanitaceae (amanita phalloides section).The ancient Ji Zong that claims, another name handle of umbrella mushroom (Sichuan), chicken silk mushroom (Taiwan, Fujian), termite mushroom etc.The activity of growing with odontoterme of collybia albuminosa has substantial connection.In a single day termite abandons nest, and he goes, and this nest just can not long again collybia albuminosa.Collybia albuminosa is distributed in the torrid areas in Africa, Asia, archipelago, the South Pacific Ocean and the subtropical zone in Asia, and also there is distribution in the Jiangsu of China, Fujian, Taiwan, Guangdong, Guangxi, Sichuan, Guizhou, Yunnan etc.
Collybia albuminosa delicious flavour, this bacterium are the rather famous and precious edible mushroom of praise of all times.Traditional Chinese medicine thinks that chicken fir flavor is sweet, property flat, nontoxic, function " beneficial stomach, clear refreshing, control hemorrhoid ", have aid digestion, refresh oneself, treat effect such as hemorrhoid.Modern medicine study proves, effects such as the chicken fir has the immune function of human body of enhancing, prevention intestinal cancer, nourishes blood, moisturizes, strengthening the spleen and stomach are applicable under poor appetite, consumptive disease palpitation, the hemorrhoid blood etc.China's successive dynasties praise that all termite collybia albuminosa U.S.A do not stop talking, and are nutritious.According to one's analysis, the bright collybia albuminosa of per 100 grams is moisture 92.61%, dry matter 7.39%.In dry matter, contain crude protein 34.94%, crude fat 3.40%, raw fiber 13.91%, soluble sugar 4.5%, hydrolysis sugar 9.59%, ash content 7.73% etc.Calcium oxide is 20.29% in ash content, phosphorus 4.62%, iron 1.89%, manganese 0.08%.Also contain ergosterol and 16 seed amino acids and vitamin C.
Modern study is found, can make stomach comfortable behind the edible chicken fir, and appetite increases, and stool and urine is unimpeded, and sleep better any side effect does not take place.Find in the teenager of national under-18s, children, to suffer from the 25%-35% that gastrointestinal dysfunction symptom in various degree accounts for China teenager, children's sum through detailed market survey.Therefore, the production high grade chook fir zymophyte powder that the present invention can be controlled in a large number can solve in the future a large amount of market demands.At present, the artificial cultivation of chicken fir fruit body not success as yet.Have not yet to see the report that chicken fir large-scale deep liquid fermentation is produced.
Summary of the invention
The objective of the invention is to produce chicken fir fermentation mycelium is target, invents a kind of chicken fir large-scale deep liquid fermentation process of Cheap highly effective, for the large-scale deep liquid fermentation production of chicken fir lays the first stone.
Realize technical scheme of the present invention: with chicken fir fungi is starting strain, adopts slant strains to carry out liquid shaking bottle cultivation, liquid shaking bottle enlarged culture, first order seed cultivation and fermented and cultured.This bacterial strain is available from Jinxiang, Shandong fungal studies institute.
Realize that concrete steps of the present invention are as follows:
(1) takes out slant strains and insert in the fresh slant medium of preparing, cultivation temperature 25-33 ℃, cultivated 100-180 hour.
(2) slant strains in the step 1 is transferred the shaking in the bottle of shake-flask culture base is housed, 25-33 ℃ of cultivation, rotating speed 120-180rpm/min cultivates the enlarged culture of shaking bottle after 40-60 hour.
(3) bottle bacterial classification that shakes in the step 2 is transferred and carried out enlarged culture again into the bottle that shakes that shakes bottle enlarged culture base is housed, 25-33 ℃ of cultivation, rotating speed 120-180rpm/min cultivates after 15-30 hour the access first class seed pot and cultivates.
(4) bottle bacterial classification that shakes in the step 3 is transferred one and is equipped with in the first class seed pot of seed culture medium, inoculum concentration is 4-10%, keep warm 25-33 ℃ in jar, tank pressure 0.5-0.9kPa, stir speed (S.S.) 80-150rpm/min, throughput 1: 0.3-0.5v/v.m fermented 30-55 hour, and ferment tank is gone in switching then.
(5) bacterial classification in the first class seed pot is transferred one be equipped with in the fermentation tank of fermentation medium, inoculum concentration is 4-10%, keeps jar warm 25-33 ℃, tank pressure 0.5-0.9kPa, stir speed (S.S.) 80-150rpm/min, throughput 1: 0.3-0.5v/v.m fermented 80-110 hour.When being reduced to the 1.0-2.0% left and right sides, content of reducing sugar puts jar.
The prescription of slant medium is (g/100ml) among the present invention: potato 10-20, glucose 1-3, agar 1.5-2.5, add water be settled to volume required, pH value nature.
The shake-flask culture base is (g/100ml) with shaking bottle prescription of enlarged culture base among the present invention: glucose 2-4, yeast extract 0.05-0.5, analysis for soybean powder 0.5-2, wheat bran juice 1.0-2.5, KH
2PO
40.1-0.5, MgSO
40.01-0.2, adding water and be settled to volume requiredly, the pH value is 4.5.
The culture medium prescription of first order seed cultivation and fermented and cultured is (g/100ml) among the present invention: glucose 2-4, yeast extract 0.05-0.5, corn flour 0.5-2, analysis for soybean powder 0.5-2, wheat bran juice 1-3, KH
2PO
40.1-0.5, MgSO
40.01-0.2, adding water and be settled to volume requiredly, the pH value is 4.5.
The shake-flask culture base is 1: 5 with shaking bottle volumetric ratio in the step 2 of the present invention, and the shake-flask culture base is 1: 5 with shaking bottle volumetric ratio in the step 3.
The volume of first class seed pot is 100-300L in the step 4 of the present invention, and the first order seed medium that is equipped with in this first class seed pot should be 50-180L mutually; The volume of fermentation tank is 1000-5000L in the step 5, and the fermentation medium that is equipped with in this fermentation tank should be 500-3500L mutually.
Jar standard of putting of the present invention is that zymotic fluid begins the thickness that becomes, and content of reducing sugar is reduced to about 1.0-2.0%, and microscopy finds that mycelium senesces.
Beneficial effect of the present invention: the present invention is applicable to that large-scale deep liquid fermentation produces the chicken fir, has solved the large-scale production of chicken fir and has cultivated a difficult problem of amplifying, and is significant for the development of chicken fir suitability for industrialized production from now on and relevant application industry.The tunning color that the present invention obtains after fermentation ends is faint yellow, gives out tempting chicken fragrance, the delicious U.S. of zymotic fluid, and it is yellow that dry mycelium is.Mycelium is counted 3.5-5.0% with dry weight in the zymotic fluid.The zymophyte powder water is decocted, the delicious smell of similar chicken is arranged.Find by analysis, contain protein 20-25% in the zymophyte powder, polysaccharide 20-25%; Methanolic extract accounts for dry weight 25-36%, and wherein, thick triterpenes components accounts for 5-6%, and Coumarins accounts for 0.7-1.2%, and the saponin class accounts for 1.5-2.2%, and all the other compositions such as oligosaccharides etc. account for 17-29%.
Embodiment
The present invention is described in further detail below in conjunction with specific embodiment.
Embodiment 1:
Inoculation collybia albuminosa filament in slant medium, 28 ℃ of cultivation temperature, incubation time 120 hours.Fresh slant strains access is equipped with in the 500ml triangular flask of 100ml medium, and 28 ℃ of shaking tables were cultivated shaking speed 150rpm/min 48 hours.Then gained is shaken transfer 500ml triangle that the 100ml medium is housed of bottle bacterial classification and shake and carry out enlarged culture in the bottle, 28 ℃ of shaking tables were cultivated shaking speed 150rpm/min 24 hours.During the bacterial classification 2200ml that triangular flask is enlarged transfers the 100L first class seed pot that the 50L seed culture medium is housed then, keep 28 ℃ of jar temperature, tank pressure 0.7kPa, stir speed (S.S.) 100rpm/min, throughput 1: 0.5v/v.m, fermentation time 45 hours.The first class seed pot bacterial classification is transferred in the 1500L fermentation tank that the 800L fermentation medium is housed, keep 28 ℃ of jar temperature, tank pressure 0.7kPa, stir speed (S.S.) 100rpm/min, throughput 1: 0.5v/v.m, fermentation time 92 hours, mycelium counts 4.6% with dry weight in the zymotic fluid.
To form be (g/100ml) to slant medium in the present embodiment 1: potato 15, and glucose 2, agar 2 adds water and is settled to volume requiredly, and the pH value is a nature.
The shake-flask culture base is (g/100ml) with shaking a bottle enlarged culture base composition in the present embodiment 1: glucose 3, yeast extract 0.2, analysis for soybean powder 1, wheat bran juice 2, KH
2PO
40.2, MgSO
40.1, adding water and be settled to volume requiredly, the pH value is 4.5.
The medium of 100L first class seed pot composition is (g/100ml) in the present embodiment 1: glucose 3, yeast extract 0.2, corn flour 1.5, analysis for soybean powder 1, wheat bran juice 2, KH
2PO
40.2, MgSO
40.1, adding water and be settled to 50L, the pH value is 4.5.
The culture medium prescription of 1500L fermentation tank is (g/100ml) in the present embodiment 1: glucose 3, yeast extract 0.2, corn flour 1.5, analysis for soybean powder 1.0, wheat bran juice 2, KH
2PO
40.2, MgSO
40.1, adding water and be settled to 800L, the pH value is 4.5.
The fermentation ends standard is in the present embodiment: zymotic fluid begins the thickness that becomes, and content of reducing sugar is reduced to 1.5%, and microscopy finds that mycelium senesces.
Claims (9)
1, a kind of chicken fir liquid deep layer fermenting production technology, it is characterized in that: with chicken fir fungi is starting strain, adopts slant strains to carry out liquid shaking bottle cultivation, liquid shaking bottle enlarged culture, first order seed cultivation and fermented and cultured.
2, chicken fir liquid deep layer fermenting production technology according to claim 1 is characterized in that: being starting strain available from Jinxiang, Shandong fungal studies institute chicken fir fungi.
3, chicken fir liquid deep layer fermenting production technology according to claim 1, it is characterized in that: it is (g/100ml) that described slant strains medium is formed: potato 10-20, glucose 1-3, agar 1.5-2.5, adding water is settled to volume required, the pH value is a nature, and 25-33 ℃ of slant strains cultivation temperature cultivated 100-180 hour.
4, chicken fir liquid deep layer fermenting production technology according to claim 1 is characterized in that: it is (g/100ml) that the medium of described shake-flask culture is formed: glucose 2-4, yeast extract 0.05-0.5, analysis for soybean powder 0.5-2, wheat bran juice 1.0-2.5, KH
2PO
40.1-0.5, MgSO
40.01-0.2, adding water and be settled to volume requiredly, the pH value is 4.5, and condition of culture is: cultivation temperature 25-33 ℃, rotating speed 120-180rpm/min cultivated 40-60 hour, and switching is gone into to shake bottle and is carried out enlarged culture then.
5, chicken fir liquid deep layer fermenting production technology according to claim 1 is characterized in that: it is (g/100ml) that the described medium that shakes bottle enlarged culture is formed: glucose 2-4, yeast extract 0.05-0.5, analysis for soybean powder 0.5-2, wheat bran juice 1.0-2.5, KH
2PO
40.1-0.5, MgSO
40.01-0.2, adding water and be settled to volume requiredly, the pH value is 4.5, and condition of culture is: cultivation temperature 25-33 ℃, rotating speed 120-180rpm/min cultivated 15-30 hour, and switching is gone into first order seed and is cultivated then.
6, chicken fir liquid deep layer fermenting production technology according to claim 1 is characterized in that: it is (g/100ml) that the medium that described first order seed is cultivated is formed: glucose 2-4, yeast extract 0.05-0.5, corn flour 0.5-2, analysis for soybean powder 0.5-2, wheat bran juice 1-3, KH
2PO
40.1-0.5, MgSO
40.01-0.2, adding water and be settled to volume requiredly, the pH value is 4.5, and inoculum concentration is 4-10%, and condition of culture is: cultivation temperature 25-33 ℃, stir speed (S.S.) 80-150rpm/min fermented 30-55 hour, and ferment tank is gone in switching then.
7, chicken fir liquid deep layer fermenting production technology according to claim 1 is characterized in that: described fermented and cultured, and it is (g/100ml) that fermentation medium is formed: glucose 2-4, yeast extract 0.05-0.5, corn flour 0.5-2, analysis for soybean powder 0.5-2, wheat bran juice 1-3, KH
2PO
40.1-0.5, MgSO
40.01-0.2, adding water and be settled to volume requiredly, the pH value is 4.5, and inoculum concentration is 4-10%, and condition of culture is: cultivation temperature 25-33 ℃, stir speed (S.S.) 80-150rpm/min fermented 80-110 hour.
8, chicken fir liquid deep layer fermenting production technology according to claim 1 is characterized in that: described fermented and cultured, and can select the fermentation tank of 1000-5000L to carry out fermented and cultured.
9, chicken fir liquid deep layer fermenting production technology according to claim 1, it is characterized in that: described fermented and cultured, mycelium is counted 3.5-5.0% with dry weight in the zymotic fluid.
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| CN 200310106081 CN1220769C (en) | 2003-10-14 | 2003-10-14 | Temilmyces fuliginosus large-scale liquid deep fermentation production process |
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| CN 200310106081 CN1220769C (en) | 2003-10-14 | 2003-10-14 | Temilmyces fuliginosus large-scale liquid deep fermentation production process |
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| CN1528118A true CN1528118A (en) | 2004-09-15 |
| CN1220769C CN1220769C (en) | 2005-09-28 |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1293818C (en) * | 2004-09-21 | 2007-01-10 | 周恒美 | Termitophile oil and its producing method |
| CN1969606B (en) * | 2006-10-16 | 2010-12-22 | 杨文兴 | Method for breeding collybia albuminosa artificially by symbiosis of termite |
| CN102487726A (en) * | 2011-12-21 | 2012-06-13 | 云南大学 | Rapid preparation method of termitomyces robustus strain |
| CN104116016A (en) * | 2013-04-23 | 2014-10-29 | 昆明翔昊科技有限公司 | Termitornyces albuminosus extractive and preparation method thereof |
| CN104285663A (en) * | 2013-07-16 | 2015-01-21 | 曲淑敏 | Black termitomyces cultivation method |
| CN105558735A (en) * | 2016-01-25 | 2016-05-11 | 四川保兴现代农业科技股份有限公司 | Edible fungus mycelium fermented drink and making method thereof |
| CN105695338A (en) * | 2016-01-25 | 2016-06-22 | 四川保兴现代农业科技股份有限公司 | Liquid spawn propagation method and field bionic cultivation method of termitomyces albuminosus |
| CN106011190A (en) * | 2016-05-17 | 2016-10-12 | 福建师范大学 | Preparation method of natural para aminobenzoic acid and derivative of natural para aminobenzoic acid |
| CN106852252A (en) * | 2016-08-12 | 2017-06-16 | 金珠满江农业有限公司 | A kind of black collybia albuminosa artificial bionic domestication and culture method |
| CN107258327A (en) * | 2017-07-31 | 2017-10-20 | 安龙县农望种植农民专业合作社 | A kind of cultural method of collybia albuminosa |
| CN111345199A (en) * | 2019-06-20 | 2020-06-30 | 四川乌蒙山四季菌业有限责任公司 | Black skin termitomyces liquid strain culture solution and preparation method thereof |
-
2003
- 2003-10-14 CN CN 200310106081 patent/CN1220769C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1293818C (en) * | 2004-09-21 | 2007-01-10 | 周恒美 | Termitophile oil and its producing method |
| CN1969606B (en) * | 2006-10-16 | 2010-12-22 | 杨文兴 | Method for breeding collybia albuminosa artificially by symbiosis of termite |
| CN102487726A (en) * | 2011-12-21 | 2012-06-13 | 云南大学 | Rapid preparation method of termitomyces robustus strain |
| CN104116016A (en) * | 2013-04-23 | 2014-10-29 | 昆明翔昊科技有限公司 | Termitornyces albuminosus extractive and preparation method thereof |
| CN104285663A (en) * | 2013-07-16 | 2015-01-21 | 曲淑敏 | Black termitomyces cultivation method |
| CN105558735A (en) * | 2016-01-25 | 2016-05-11 | 四川保兴现代农业科技股份有限公司 | Edible fungus mycelium fermented drink and making method thereof |
| CN105695338A (en) * | 2016-01-25 | 2016-06-22 | 四川保兴现代农业科技股份有限公司 | Liquid spawn propagation method and field bionic cultivation method of termitomyces albuminosus |
| CN106011190A (en) * | 2016-05-17 | 2016-10-12 | 福建师范大学 | Preparation method of natural para aminobenzoic acid and derivative of natural para aminobenzoic acid |
| CN106011190B (en) * | 2016-05-17 | 2020-02-07 | 福建师范大学 | Preparation method of natural p-aminobenzoic acid and derivatives thereof |
| CN106852252A (en) * | 2016-08-12 | 2017-06-16 | 金珠满江农业有限公司 | A kind of black collybia albuminosa artificial bionic domestication and culture method |
| CN107258327A (en) * | 2017-07-31 | 2017-10-20 | 安龙县农望种植农民专业合作社 | A kind of cultural method of collybia albuminosa |
| CN111345199A (en) * | 2019-06-20 | 2020-06-30 | 四川乌蒙山四季菌业有限责任公司 | Black skin termitomyces liquid strain culture solution and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN1220769C (en) | 2005-09-28 |
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