CN1500400A - Enzyme-enriched prebiotics and its preparation - Google Patents
Enzyme-enriched prebiotics and its preparation Download PDFInfo
- Publication number
- CN1500400A CN1500400A CNA021452504A CN02145250A CN1500400A CN 1500400 A CN1500400 A CN 1500400A CN A021452504 A CNA021452504 A CN A021452504A CN 02145250 A CN02145250 A CN 02145250A CN 1500400 A CN1500400 A CN 1500400A
- Authority
- CN
- China
- Prior art keywords
- enzyme
- enriched
- prebiotic
- animal
- carbon source
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
Abstract
The enzyme-rich bioactive article contains complex bacteria seeds, culture medium, carbon source, nitrogen source and inducing agent. It features the bacteria seed screened from animal intestinal tract; the compound culture of the bacteria seeds in the same culture medium; compound sync solid fermentation to form powerful compound microbe colony and effective enzyme generating capacity resulting in great amount of active enzyme, small peptide, antioxidant matter, free amino acid, vitamins and other physiological matter in the product; and double-layer microcapsule coating treatment of thermolabile bacteria seed. It is used in promoting the digestion and absorption of animal to feed, promoting the synthesis and full utilization of protein in animal body, strengthening animal's resistance and immunity, raising feed converting rate and reducing the pollution of animal's excrement.
Description
Technical field:
The present invention relates to a kind of fodder additives and making method of enzyme-enriched prebiotic, belong to field of feed additive technology.
Background technology:
At present, disclosed patent, publication number is CN00133695, be entitled as biology feed additive and preparation method thereof, it is characterized in that: by screening lactobacillus viridescens, the pure bacterium of lactobacillus coprophilus, yeast, subtilis through enlarged culturing, secondary enlarged culturing, with rice bran, wheat bran adsorption liquid heap fermentation, oven dry and reach bacterial classification quantity 8-10 hundred million/gram.This biology feed additive bacterial classification is a single culture only, and viable count is low, and other physiological metabolism material yields poorly.
Summary of the invention:
Purpose of the present invention is exactly that a kind of probiotics of invention is counted height, enzymatic productivity is strong and the additive of materials such as tool various active enzyme and little peptide.After interpolation is fed, can form the optimum ecotope of animal body, promote conversion absorption, the enhance immunity power of nutritive substance, the enzyme-enriched prebiotic and the making method that can be applicable to the feed granulating production technique also are provided simultaneously.
For realizing above purpose, technical scheme of the present invention provides a kind of enzyme-enriched prebiotic, its characteristics are, take the bilayered microcapsule bag processed to the thermo-labile bacterial classification of composite bacteria that is used for the feed granulating use, take modification technique with tolerance granulation high temperature to organized enzyme; Adopt the composite sync solid-fermented technique, make that the output of organized enzyme and little peptide significantly improves in the product, also contain physiological metabolism materials such as a large amount of antioxidant, VITAMIN, Methionin simultaneously.Raw material is made up of composite bacteria stoste, carbon source, nitrogenous source, inorganic salt, inductor, and it is formulated by following weight percentages:
Composite bacteria stoste 10-40%
Carbon source 30-70%
Nitrogenous source 10-35%
Inorganic salt 0.1-5%
Inductor 0.02-1%
The optimum weight proportioning of each raw material of preparation of the present invention is:
Composite bacteria stoste 20%
Carbon source 60%
Nitrogenous source 20%
Inorganic salt 1%
Inductor 0.1%
Composite bacteria of the present invention is one of Rhodopseudomonas palustris, plant lactobacillus, streptococcus uberis, saccharomyces cerevisiae, subtilis, bifidus bacillus, lactobacterium casei or their mixture, when being used for feed granulating thermo-labile bacterial classification is carried out bilayer bag quilt with gum arabic, gelatin; Described carbon source comprises one of starch, Mierocrystalline cellulose or their mixture, and wherein starch is for keeping the active most important of enzyme when the feed granulating; Described nitrogenous source be soybean cake powder, peanut cake, fish meal, dried silkworm chrysalis meal, yeast powder, corn steep liquor, one of or their mixture; Nitrogenous source decomposes under the organized enzyme effect that is produced by bacterial metabolism as nutritive substance, can form little peptide and total free aminoacids; Described inorganic salt are one of ammonium salt, nitrate or their mixture; Described inductor is wooden sophorose, is mainly used in the output that improves organized enzyme.
Making method of the present invention is characterized in that, all bacterial classifications of employing all are to screen from animal intestinal, utilizes the protoplasma integration technology preferably to come out; Adopt the bacterial classification compound culture method, all bacterial classifications are cultivated in same substratum, it comprises the following steps:
(1) strain screening from animal intestinal utilizes the protoplasma integration technology to be optimized;
(2) be to cultivate in molasses, peptone, glucose, sodium-chlor, yeast extract paste, the sal epsom all bacterial classifications after the segmentation optimization cultivation at same substratum, the ph value is to 6.8-7.2; Culture temperature is that 30-35 ℃, time are 3-4 days, obtains composite fungus agent; When being used for feed granulating, need in the microbial inoculum to add gum arabic, gelatin, wrap processed;
(3) composite fungus agent is added insert in the closed tin behind carbon source, nitrogenous source, inorganic salt and the inductor uniform mixing and ferment, 30-35 ℃ bottom fermentation 6-8 days, utilize the composite sync solid-fermented technique, solved the growth velocity problem different that causes each bacterial classification owing to reasons such as nutrition, pH value, redox potential EH, ionic strength and culture temperature, the anaerobism of these multiple different generas and aerobic beneficial microorganism are fermented synchronously with ratio; Drying obtains pulverous enzyme-enriched prebiotic finished product after crushed.
The present invention adopts and is mixed with organic nutrient substance by the multiple bacterial classification that filters out from animal intestinal, make by microbial fermentation technology, it contains various active enzyme, little peptide, total free aminoacids, monomeric protein, antioxidant, VITAMIN and antimicrobial substance, and the quantity of probiotics viable count has reached 10,000,000,000/gram; Aspartic protease 6500u/g, zytase 10000u/g, cellulase 9000u/g, beta-glucanase 8000u/g, polygalacturonase 10000u/g, phytase 650u/g; Active small peptide content is greater than 25%.
The flora of in the enteron aisle of normal livestock and poultry, surviving and constituting by various kinds of cell, after the beneficial microorganism in the enzyme-enriched prebiotic enters the enteron aisle of livestock and poultry and fishery products, in conjunction with original probiotics, form the superior microorganism flora rapidly, suppressed growth of harmful intestinal tract bacteria, beneficial microorganism is wherein combined closely by teichoic acid and intestinal epithelial cell around the stomach wall, forms the mycoderm barrier, stops the field planting of harmful bacterium; Complex microorganism also can produce antimicrobial substance, acidophilin, lactolin, bacitracin etc., suppress breeding, field planting and the absorption of harmful bacterium in enteron aisle such as intestinal bacteria, Salmonellas, in and toxic product, suppress the synthetic of ammonia and amine, promote the height response capacity of gut-associated lymphoid tissue, immune organ is grown speed, immunity system is ripe early, fast, and the quantity of T, bone-marrow-derived lymphocyte increases, make body fluid and the cellular immune level of animal improve the enhancing body resistance against diseases.
Proteolytic enzyme contained in the enzyme-enriched prebiotic can be hydrolyzed into little peptide or amino acid with the macromole polypeptide of constitutive protein; Tie up plain enzyme, polygalacturonase can be abolished plant cell wall, and entocyte is fully discharged, by the monogastric animal enteron aisle is absorbed; Beta-glucanase, zytase, energy hydrolysis water dissolubility beta-glucan, xylan, effectively reduce chyme viscosity in the animal intestinal, help the abundant and chyme mixing of endogenous Digestive system, fully digestion, reduce livestock and poultry diarrhea, be beneficial to utilization ratio and energy that nutraceutical absorption improves feed, reduce the material anharmonic ratio; Phytase can transform to orthophosphoric acid salt, inositol and inositol derivative by the catalysis phytic acid, phosphorus hydrolysis in the phytic acid is discharged, make phosphorus wherein must think animal use, can reduce like this and add inorganic acid salt, reduce the discharging of ight soil phosphorus, alleviate pollution, but also can improve combined protein, the utilization of mineral element, improve digestibility environment.Enzyme-enriched prebiotic also can reduce the generation of ammonia and other corrupt substance, ammonia amount in intestinal contents, ight soil and the quiet portal vein is descended, first cresols Yin once repugnant substance such as increased with 3-methyl Yin reduce in the intestinal contents, thus the foul smell that ight soil produces reduced, reach deodorizing effect.
A large amount of little peptide materials can be eliminated the absorption competition of total free aminoacids in the enzyme-enriched prebiotic, accelerate the synthetic of animal body internal protein, improve proteinic sedimentation effect, promote the absorption and the utilization of mineral element, and some peptide has antioxygenation, by the decomposition of removing heavy metal ion and promoting to become the superoxide of free radical, reduce the peroxide level of automatic oxidation rate and fat, can significantly improve the production performance and the immunological competence of feeding animals.
Enzyme-enriched prebiotic adopts carbon source, nitrogenous source, inorganic salt and inductor as the spawn culture raw material, and they are important component parts of microorganism cells structure and enzyme, also can keep the activity of enzyme, can promote the microorganism growth breeding, the generation of stimulating activity enzyme and little peptide.
Advantage of the present invention is:
1. powerful enzymatic productivity activates the secretion of endogenous enzyme, has remedied the interior endogenous enzyme of animal body and reach quantitative deficiency on kind, promotes animal digesting and assimilating feed;
2. exogenous enzyme is provided, eliminate in the diet anti-nutrition because of;
3. competent little peptide material is provided, promotes the abundant absorption of nutritive substances such as body protein, significantly improve production performance;
4. effectively decompose the macromole nutritive substance that some animals are difficult for absorption, improve the utilization ratio of the nutritive value of inferior forage component;
5. by the synergy of various antioxidant, physiological metabolism material and enzyme, improve resistance against diseases and the immunological competence of livestock and poultry;
6. improve food conversion ratio, alleviate the pollution of ight soil environment;
7. alternative is the microbiotic use of purpose with prevention and promotes growth, produces the organic farm products of green, safety.
Embodiment
1. the making method of enzyme-enriched prebiotic agent:
(1). strain screening from animal intestinal takes by weighing by following weight proportion:
Rhodopseudomonas palustris 12%, plant lactobacillus 15%, streptococcus uberis 5%, saccharomyces cerevisiae 20%, subtilis 20%, bifidus bacillus 18%, buttermilk bacillus 10%;
Take by weighing substratum by following weight proportion:
Molasses 0.6%, peptone 0.9%, sodium-chlor 0.4%, glucose 0.1%, yeast extract paste 0.5%, sal epsom 0.06%, ph value are adjusted to 6.8;
(2). is to cultivate in molasses, peptone, glucose, sodium-chlor, yeast extract paste, the sal epsom all bacterial classifications at same substratum, ph value to 6.8; Culture temperature is that 30 ℃, time are 4 days, obtains composite fungus agent.When being used for feed granulating, need to add gum arabic, gelatin in the microbial inoculum, wrap processed thermo-labile bacterial classification;
Take by weighing composite fungus agent and raw material by following weight proportion:
It is that wooden sophorose 0.1%, inorganic salt are ammonium sulfate 1% that composite fungus agent 20%, carbon source select for use Semen Maydis powder 60%, nitrogenous source to select soybean cake powder 20%, inductor for use;
(3). insert in the closed tin after composite fungus agent added carbon source, nitrogenous source, inorganic salt and inductor uniform mixing, 30-35 ℃ of bottom fermentation 7, it utilizes the composite sync solid-fermented technique, solved the growth velocity problem different that causes each bacterial classification owing to reasons such as nutrition, pH value, redox potential EH, ionic strength and culture temperature with ratio, the anaerobism and the aerobic beneficial microorganism of these how sharp different generas are fermented synchronously, and drying obtains pulverous enzyme-enriched prebiotic after crushed.
In use, evenly mixing and stirring the back according to different livestock and poultry in different ratios and feed feeds: the fowl poultry kind animal is in 3 ‰ ratios; The aquatic product animal is in 2 ‰ ratios.
When being used for feed granulating, the enzyme-enriched prebiotic tailored version of granulating is added in feed or the Preblend in the ratio of 1-3 ‰ and granulates.
Claims (8)
1. an enzyme-enriched prebiotic is characterized in that, is formed by the composite sync solid fermentation by raw materials such as composite bacteria stoste, carbon source, nitrogenous source, inorganic salt, inductors, and it is prepared by following weight percentages:
Composite bacteria stoste 10-40%
Carbon source 30-70%
Nitrogenous source 10-35%
Inorganic salt 0.1-5%
Inductor 0.02-1%
2. an enzyme-enriched prebiotic is characterized in that, wherein the optimum weight proportioning of each raw material is:
Composite bacteria stoste 20%
Carbon source 60%
Nitrogenous source 20%
Inorganic salt 1%
Inductor 0.1%
3. enzyme-enriched prebiotic according to claim 1, its characteristics are that described composite bacteria is one of Rhodopseudomonas palustris, plant lactobacillus, streptococcus uberis, saccharomyces cerevisiae, subtilis, bifidus bacillus, lactobacterium casei or their mixture.
4. enzyme-enriched prebiotic according to claim 1, its characteristics are that described carbon source comprises one of starch, Mierocrystalline cellulose, Semen Maydis powder or their mixture.
5. enzyme-enriched prebiotic according to claim 1, its characteristics are that described nitrogenous source is one of soybean cake powder, peanut cake, fish meal, dried silkworm chrysalis meal, yeast powder, corn steep liquor or their mixture.
6. enzyme-enriched prebiotic according to claim 1, its characteristics are that described inorganic salt are one of ammonium salt, nitrate or their mixture.
7. enzyme-enriched prebiotic according to claim 1, its characteristics are that described inductor is wooden sophorose.
8. enzyme-enriched prebiotic and making method is characterized in that all bacterial classifications of employing all filter out from animal intestinal; Adopt the bacterial classification compound culture method, all bacterial classifications are cultivated in same substratum, it comprises the following steps:
(1) strain screening from animal intestinal utilizes the protoplasma integration technology to carry out preferably;
(2) be to cultivate in molasses, peptone, glucose, sodium-chlor, yeast extract paste, the sal epsom each bacterial classification after the segmentation cultivation at same substratum, the ph value is to 6.8-7.2; Culture temperature is that 30-35 ℃, time are 3-4 days, obtains composite fungus agent; When being used for feed granulating, need to add gum arabic, gelatin in the microbial inoculum, wrap processed heat labile lactobacillus, bifidus bacillus etc.;
(3) insert in the closed tin after composite fungus agent being added carbon source, nitrogenous source, inorganic salt and inductor uniform mixing, 30-35 ℃ bottom fermentation 6-8 days, utilize the composite sync solid-fermented technique, solved the growth velocity problem different that causes each bacterial classification owing to reasons such as nutrition, pH value, redox potential EH, ionic strength and culture temperature, the anaerobism of these multiple different generas and aerobic beneficial microorganism are fermented synchronously with ratio; Drying obtains pulverous enzyme-enriched prebiotic finished product after crushed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021452504A CN1283170C (en) | 2002-11-12 | 2002-11-12 | Enzyme-enriched prebiotics and its preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021452504A CN1283170C (en) | 2002-11-12 | 2002-11-12 | Enzyme-enriched prebiotics and its preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1500400A true CN1500400A (en) | 2004-06-02 |
| CN1283170C CN1283170C (en) | 2006-11-08 |
Family
ID=34232341
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021452504A Expired - Fee Related CN1283170C (en) | 2002-11-12 | 2002-11-12 | Enzyme-enriched prebiotics and its preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1283170C (en) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007085142A1 (en) * | 2006-01-27 | 2007-08-02 | Chengde Ciyuan Mining Industry Group, Ltd | Animal fermented concentrated feed and compound feed, manufacturing methods, and apparatus thereof |
| CN101885640A (en) * | 2010-06-25 | 2010-11-17 | 上海创博生态工程有限公司 | Method for preparing mushroom medium by using microbial composite bacteria |
| CN101099543B (en) * | 2007-08-03 | 2010-11-17 | 上海拜森生物技术有限公司 | Aquatic biological feed and preparation method thereof |
| CN101940267A (en) * | 2010-09-09 | 2011-01-12 | 上海创博生态工程有限公司 | Method for preparing microbial fermentation feed for improving pork quality |
| CN102040405A (en) * | 2010-10-11 | 2011-05-04 | 山东郁茏生物科技有限公司 | Fish protein polypeptide biological bacterial fertilizer |
| CN102210375A (en) * | 2010-04-09 | 2011-10-12 | 广州市利生源生物饲料有限公司 | Preparation method of microbial fermentation feed |
| CN103421710A (en) * | 2013-07-29 | 2013-12-04 | 内蒙古和美科盛生物技术有限公司 | Lactobacillus casei, bifidobacterium animalis and lactobacillus plantarum for solid fermentation of probiotics in field of feed additives |
| CN103436466A (en) * | 2013-07-29 | 2013-12-11 | 内蒙古和美科盛生物技术有限公司 | Lactobacillus casei and bacillus subtilis for probiotic solid fermentation in field of feed additives |
| CN104938868A (en) * | 2015-07-22 | 2015-09-30 | 江苏天成科技集团有限公司 | Fermented feed capable of improving laying performance and egg quality of laying hens |
| CN105219758A (en) * | 2015-11-04 | 2016-01-06 | 梅庆波 | A kind of preparation method of feeding slowly-releasing immobilized cellulase |
| CN105851484A (en) * | 2016-03-24 | 2016-08-17 | 宋介珍 | Microbial feed additive, and preparation process and application thereof |
| CN107889934A (en) * | 2017-11-22 | 2018-04-10 | 李九玲 | A kind of animal health potus for promoting growth |
| CN108913617A (en) * | 2018-06-29 | 2018-11-30 | 上海创博生态工程有限公司 | A kind of biological agent and preparation method thereof of efficient-decomposition organic waste |
-
2002
- 2002-11-12 CN CNB021452504A patent/CN1283170C/en not_active Expired - Fee Related
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007085142A1 (en) * | 2006-01-27 | 2007-08-02 | Chengde Ciyuan Mining Industry Group, Ltd | Animal fermented concentrated feed and compound feed, manufacturing methods, and apparatus thereof |
| CN101099543B (en) * | 2007-08-03 | 2010-11-17 | 上海拜森生物技术有限公司 | Aquatic biological feed and preparation method thereof |
| CN102210375A (en) * | 2010-04-09 | 2011-10-12 | 广州市利生源生物饲料有限公司 | Preparation method of microbial fermentation feed |
| CN101885640A (en) * | 2010-06-25 | 2010-11-17 | 上海创博生态工程有限公司 | Method for preparing mushroom medium by using microbial composite bacteria |
| CN101885640B (en) * | 2010-06-25 | 2013-02-13 | 上海创博生态工程有限公司 | Method for preparing mushroom medium by using microbial composite bacteria |
| CN101940267A (en) * | 2010-09-09 | 2011-01-12 | 上海创博生态工程有限公司 | Method for preparing microbial fermentation feed for improving pork quality |
| CN101940267B (en) * | 2010-09-09 | 2013-05-29 | 上海创博生态工程有限公司 | Method for preparing microbial fermentation feed for improving pork quality |
| CN102040405A (en) * | 2010-10-11 | 2011-05-04 | 山东郁茏生物科技有限公司 | Fish protein polypeptide biological bacterial fertilizer |
| CN103421710A (en) * | 2013-07-29 | 2013-12-04 | 内蒙古和美科盛生物技术有限公司 | Lactobacillus casei, bifidobacterium animalis and lactobacillus plantarum for solid fermentation of probiotics in field of feed additives |
| CN103436466A (en) * | 2013-07-29 | 2013-12-11 | 内蒙古和美科盛生物技术有限公司 | Lactobacillus casei and bacillus subtilis for probiotic solid fermentation in field of feed additives |
| CN103436466B (en) * | 2013-07-29 | 2015-09-09 | 内蒙古和美科盛生物技术有限公司 | For lactobacterium casei and the subtilis of the probiotic solid fermentation of feed additive field |
| CN103421710B (en) * | 2013-07-29 | 2015-09-16 | 内蒙古和美科盛生物技术有限公司 | For the lactobacterium casei of fodder additives probiotic solid fermentation, animal bifidobacteria and plant lactobacillus |
| CN104938868A (en) * | 2015-07-22 | 2015-09-30 | 江苏天成科技集团有限公司 | Fermented feed capable of improving laying performance and egg quality of laying hens |
| CN105219758A (en) * | 2015-11-04 | 2016-01-06 | 梅庆波 | A kind of preparation method of feeding slowly-releasing immobilized cellulase |
| CN105851484A (en) * | 2016-03-24 | 2016-08-17 | 宋介珍 | Microbial feed additive, and preparation process and application thereof |
| CN107889934A (en) * | 2017-11-22 | 2018-04-10 | 李九玲 | A kind of animal health potus for promoting growth |
| CN108913617A (en) * | 2018-06-29 | 2018-11-30 | 上海创博生态工程有限公司 | A kind of biological agent and preparation method thereof of efficient-decomposition organic waste |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1283170C (en) | 2006-11-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101449744B (en) | Multifunctional biology aquatic feedstuff additive | |
| CN102100302B (en) | Preparation method of fermentation cake and maggot protein feed | |
| CN101186896A (en) | Fermentation material for composite microorganism and preparation method thereof | |
| CN102210413B (en) | Dorking microorganism viable bacteria fermentation concentrated feed and production method thereof | |
| CN1813562A (en) | Compound microbial fermented fodder nutrient additive and its preparing method | |
| CN101209088A (en) | Microorganism polyzyme additive agent for improving pigling growth and development | |
| CN1283170C (en) | Enzyme-enriched prebiotics and its preparation | |
| CN103907747A (en) | Preparing method of high-protein feed | |
| CN1284471C (en) | Active biological fodder additive | |
| CN102511688A (en) | Microcapsule biological feed | |
| CN101073585A (en) | Microbial preparation for improving eel liver growth and its making method | |
| CN112471325A (en) | Straw biological fermentation feed and preparation method and application thereof | |
| CN113974013A (en) | Fungus chaff fermented feed applied to laying hen breeding | |
| CN1190145C (en) | Process for producing stalk protein feed by multi-culture united anaerobic fermentation | |
| CN106035990B (en) | Method for preparing biological feed by solid-state enzymolysis fermentation of citrus pulp, product and application thereof | |
| CN107006677A (en) | A kind of feed and its application rich in probiotics and active peptide | |
| CN102429108A (en) | Viable microbe fermentation compound feed for layer chickens in laying period and production method thereof | |
| KR20180075986A (en) | A Method for functional fermentation composition | |
| CN113414226A (en) | Kitchen waste leavening agent and preparation method and application thereof | |
| CN101690541B (en) | Method for preparing feed protein from microbial fermented silkworms | |
| RU2546880C2 (en) | Method of probiotic feed preparation for farm animals | |
| CN110140814A (en) | A kind of class fermented type mixed feed and the preparation method and application thereof | |
| CN114190486A (en) | Microbial fermented feed for preventing and treating constipation of sows and sow breeding method | |
| CN1806656A (en) | Elm tree mushroom feedstuff addictive, its preparation method and application | |
| CN104206671A (en) | Method for preparing biological feed from entire plant of sugarcane |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20061108 Termination date: 20211112 |