CN1480532A - Gene of cortexin-3 receptor of pig melanin and method for detecting polymorphism of mononucleotide - Google Patents
Gene of cortexin-3 receptor of pig melanin and method for detecting polymorphism of mononucleotide Download PDFInfo
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- CN1480532A CN1480532A CNA021390010A CN02139001A CN1480532A CN 1480532 A CN1480532 A CN 1480532A CN A021390010 A CNA021390010 A CN A021390010A CN 02139001 A CN02139001 A CN 02139001A CN 1480532 A CN1480532 A CN 1480532A
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Abstract
A process for detecting pig melanocortin-3 receptor (MC3R) gene and its single nucleotide polymorphism (SNP) includes extracting DNA from pig blood genom, designing primer based on human and mouse MC3R gene conservative region, PCR amplification, directly sequencing the PCR product, comparing sequence, analyzing and detecting SNP.
Description
Technical field
The invention belongs to the technical field of molecular biology of pig, relate to the clone of pig MC3R gene and the detection method of order-checking and single nucleotide polymorphism.Relevant with the molecular engineering breeding of pig.
Technical background
Melanocortin receptor is G-protein coupling receptor (G-protein coupled receptors, GPCRs) one of member of super tame √ √ family.So far, 5 MCRs hypotypes (MC1R-MC5R) of people, mouse and chicken are cloned and are identified that its tissue distribution and biological function have nothing in common with each other.
MC3R is a clone and an evaluation acceptor the latest among 5 MCRs, and the MC3R gene of people, mouse and chicken is cloned and identified, 360,323 and 325 amino acid of encoding respectively.Coding people MC3R gene locus is positioned in human chromosome 20q, this site and management of body weight index (body mass index), and subcutaneous lipids amount and fasting insulin level are chain.A nearest quantitative trait locus that influences estrogen level also is positioned in this zone, but people are still not clear to the understanding of MC3R function.MC3R may be receptor related with the candidate gene that does not rely on the Regular Insulin obesity, perhaps the adjusting behavior of searching for food played an important role.In order to determine the effect of MC3R in energy homeostasis, people such as Butler delete most MC3R gene coded sequences from the musculus cdna group, and with knocking out the MC4R dna rat in contrast.Result of study shows: MC3R
-/-(MC3R gene knockout) mouse presents exclusive metabolism syndrome, though its body weight does not significantly increase, lipid content increases by 50% to 60%, and reduces energy expenditure.In view of the above, people such as Butler think that MC3R may participate in regulating energy homeostasis (.2000 such as Butler AA, A unique metabolic syndrome causes obesity in the melanocortin-3receptor-deficient mouse.Endocrinology.141 (9): 3518-21).People's such as Chen research is also found: with wild-type mice relatively, and 4-6 monthly age MC3R-/-increase of mouse body fat content, cutability reduces, and feed efficiency is higher, and body weight does not have difference; Lack MC3R and MC4R dna rat simultaneously, because MC3R genetically deficient, the obesity that MC4R genetically deficient causes (.2000 such as Chen AS, Inactivation of the mouse melanocortin-3 receptor results inincreased fat mass and reduced lean body mass.Nat Genet.26 (1): 97-102) have been aggravated.By high body weight and the analysis and research of the hybridization chicken MC3R of under-weight system gene genetics, found 5 new single nucleotide polymorphism, set up MC3R gene PCR-rflp analysis technology.Analyze for chicken by 762 F2, there are significant correlation in this gene pleiomorphism and chicken body weight (2,4,6,8 and 10 age in week) and cock abdomen fat, can be used as the candidate gene (Jiang Siwen etc. of chicken body weight, 2002, reference family chicken melanocortin receptor 3 gene pleiomorphisms and weight relation research. Acta Genetica Sinica, No.4:322～325).
Same people, mouse and chicken research are compared, and pig MCRs research report seldom.1998, Andersson and colleague thereof reported the relation of MC1R transgenation and appearance color, had disclosed 4 allelic variation (E by sequential analysis
+, E
D, E
P, e).E
+Control aper hair color is wild-type appearance color; E
D1Control black (big black pig in Europe and Mei Shan bristles color); E
D2Control black has leukorrhea (kind of pig is a hampshire); E
PControl white (kind of pig is a Large White) or white have blackspot (kind of pig is a Pietrain); Red (kind of pig is Du Luoke) (Kijas etc., 1998, Melanocortin receptor 1 (MC1R) the mutations and coat color in pigs.Genetics.150 (3): 1177-85) of e control.The MC2R gene of people such as Hiendleder (1999) personnel selection is made probe, has found that pig MC2R gene is based on SacI enzyme RFLP polymorphism.MC4R illustrates in the search for food vital role of behavior and body weight of control people and mouse, has caused the research of MC4R as the important production traits candidate gene of pig.332 amino acid of pig MC4R genes encoding are positioned at karyomit(e) No. 1, with microsatellite marker S03113 and S0082 close linkage.By sequence comparing analysis, found a nonsense mutation site, and the PCR-RFLP that has set up based on the TaqI restriction enzyme differentiates pig MC4R genotype method.By being market pig from 5 in PIC company, totally 1800 analyses, show MC4R genotype and the thickness of backfat, growth rate and food consumption significant correlation, can be used as the candidate gene relevant with fat proterties, also can be used as the animal model (Kim etc. of human diseases research research, 2000, A missense variant of the porcine melanocortin-4receptor (MC4R) gene is associated with fatness, growth, and feed intake traits.Mamm Genome.11 (2): 131-5).People such as Kim have also reported pig MC5R gene pleiomorphism, chain and physical map.Liu Guilan etc. (2002) adopt the PCR-RFLP technology, have analyzed the TaqI endonuclease bamhi polymorphism of MC4R Gene Partial fragment in pig resource family colony and have distributed.The result of the correlation analysis of MC4R gene pleiomorphism and grow-finish proterties, meat proterties, carcass trait shows, the distribution difference of MC4R genotype frequency in different varieties colony; Fat thickness, buttocks fat thickness, average back fat, eye muscle width, eye muscle area, skin rate are the relevant (Liu Guilan etc. of significance between MC4R gene and pig chest lumbar vertebrae, 2002, pig resource family MC4R genescan and with the correlation analysis of fatty character. Acta Genetica Sinica, No.6:497～501).But any report is not seen in pig MC3R gene studies as yet.
Summary of the invention
The objective of the invention is to obtain pig MC3R gene (DNA), obtain single nucleotide polymorphism by sequence comparing analysis; Set up suitable single nucleotide polymorphism classifying method in view of the above, provide the molecule marker of usefulness for the marker assisted selection of pig feed intake and energy homeostasis.
The present invention is achieved through the following technical solutions:
(its coding nucleotide sequence is shown in SEQ ID No.1 and sequence table SEQ ID No.2 for melanocortin-3 receptor, MC3R) gene for pig melanocortin-3 acceptor.480 in Nucleotide in the amplification of MC3R gene PCR has a single nucleotide polymorphism (SNP).
A kind of method for preparing pig melanocortin-3 acceptor (MC3R) gene, according to following steps: extract DNA from the pig blood genome, according to people and mouse MC3R gene conserved regions design primer, pcr amplification, PCR product purification and directly order-checking are by the nucleotide sequence of sequence comparing analysis acquisition shown in sequence table SEQ ID No.1 and sequence table SEQ ID No.2.
The method that is used for the detection of pig MC3R gene SNP somatotype is to adopt the two-way pcr amplification of special allelotrope (Bi-PASA) step.In order to realize aforesaid method, designed the dna sequence dna of four kinds of primers, they are respectively shown in sequence table SEQ ID No.3, SEQ ID No.4, SEQ ID No.5, SEQ ID No.6:
Sequence table SEQ ID No.3:
F
1：5’-GCC?TTC?TGT?GAG?CAG?GTC?TTC-3’
Sequence table SEQ ID No.4:
R
1：5’-GAC?GGA?GTT?GCA?CAT?GAT?GAG-3’
Sequence table SEQ ID No.5:
F
2：5’-AAG?ATG?GTC?ATC?GTG?TGC?CTT-3’
Sequence table SEQ ID No.6:
R
2：5’-GGC?GAA?GAA?GAT?GAC?GAC?G-3’
The clone and the order-checking of pig MC3R gene, its step comprises: selection place of china kind blood lineage's plum mountain pig and adventive Large White are as the typical test material, from the blood of pig, extract genomic dna, according to people and mouse MC3R gene conserved regions design two outer primer (F
1: 5 '-GCC TTC TGT GAG CAG GTC TTC-3 ', R
1: 5 '-GAC GGA GTT GCA CAT GAT GAG-3 '), pcr amplification, PCR product purification and directly order-checking, sequence comparing analysis.It is characterized in that obtaining pig MC3R gene coding region partial dna sequence.Wherein plum mountain pig and Large White MC3R gene DNA sequence are seen sequence table respectively: SEQ ID No.1. and sequence table: shown in the SEQ ID No.2.
Comparative sequences table SEQ ID No.1. and sequence table: the dna sequence dna of SEQ ID No.2, found the SNP site of 480 C → T of Nucleotide of MC3R gene PCR amplified production, adopt the two-way pcr amplification of special allelotrope (bi-directional PCR amplification of specific alleles in view of the above, Bi-PASA) method has been set up MC3R gene SNP pleiomorphism detecting method.The base sequence that it is characterized in that special outer primer and inner primer as mentioned above.
Sequence table and explanation thereof:
1, sequence table SEQ ID No.1: be the coding nucleotide sequence that derives from Chinese native pig breed blood lineage " plum mountain pig " clone;
2, sequence table SEQ ID No.2: be the coding nucleotide sequence that derives from external pig kind " Large White " clone;
3, sequence table SEQ ID No.3: be the nucleotide sequence of implementing the used special outer primer of MC3R gene SNP pleiomorphism detecting method;
4, sequence table SEQ ID No.4: be to implement the used special outer primer nucleotide sequence of MC3R gene SNP pleiomorphism detecting method;
5, sequence table SEQ ID No.5): be to implement the used special inner primer nucleotides sequence of MC3R gene SNP pleiomorphism detecting method;
6, sequence table SEQ ID No.6: be implement MC3R gene SNP pleiomorphism detecting method the nucleotide sequence of special inner primer.
Accompanying drawing and explanation thereof:
Fig. 1: be that pig and people MC3R gene order compare;
Fig. 2: the partial nucleotide sequence of pig MC3R gene and SNP site.The present invention has following effect:
Genetic marker of the present invention and SNP method for quick can be used for the MC3R Polymorphism Analysis of different pig varieties, and the relation of research gene and pig feed intake and energy homeostasis.
Embodiment
Embodiment 1:
Select place of china kind plum mountain pig and external (abroad) kind Large White as the typical test material, adopt the comparative genomics method, according to people and mouse MC3R gene conserved regions design primer (outer primer).
Upstream primer F
1: 5 '-GCC TTC TGT GAG CAG GTC TTC-3 '
Downstream primer R
1: 5 '-GAC GGA GTT GCA CAT GAT GAG-3 '
PCR is reflected at and contains 50ng genomic dna, 15mmol/L Tris-HCl, 50mmol/L KCl (pH8.0), 2mmol/L MgCl
2, 200 μ mol/L dNTPs, primers F
1And R
1Carry out in the 25 μ l reaction systems of 5 μ mol/L, 4% methyl-sulphoxide and 0.5 U TaqDNA polysaccharase, reaction conditions is 95 ℃ of 5min, 35 circulations (94 ℃ of 45S, 60 ℃ of 45S and 72 ℃ of 90S), 72 ℃ of prolongation 5min.After being purified, amplification PCR products (783bp) directly carries out sequencing.Dna sequence dna Sequencher
TM3.0 software (Gene Codes Corporation) is arranged and compared, plum mountain pig and Large White MC3R gene DNA sequence are seen sequence table SEQ ID No.1 and sequence table SEQ ID No.2.With BLAST software relatively, the sequence of PCR product conclusive evidence PCR product is the MC3R gene, with corresponding people's sequence 90% homology (see figure 1) is arranged on dna level.
Embodiment 2
Through determined dna sequence and sequence comparing analysis, at the Nucleotide 480 of MC3R gene PCR amplified production
The position has found that (C → T), i.e. SNP (see figure 2): pig is C to 1 sequence change on the kind plum mountain of pig, and Large White is T.In view of the above, design special inner primer (F
2: 5 '-AAG ATG GTC ATC GTG TGC CTT-3 ', R
2: 5 '-GGCGAAGAAGATGACGACG-3 '), with outer primer (F
1: 5 '-GCCTTCTGTGAGCAGGTCTTC-3 ', R
1: 5 '-GACGGAGTTGCACATGATGAG-3 ') together, adopt the Bi-PASA analytical technology to carry out the SNP somatotype.PCR is reflected at and contains 50ng genomic dna, 15mmol/L Tris-HCl, 50mmol/L KCl (pH8.0), 2mmol/L MgCl
2, 200 μ mol/L dNTPs, outer primer F
1And R
15 μ mol/L, inner primer F
2And R
22.5 carry out in the 25 μ l reaction systems of μ mol/L, 4% methyl-sulphoxide and 0.5 U Taq archaeal dna polymerase, reaction conditions is 95 ℃ of 5min, 35 circulations (94 ℃ of 60S, 65 ℃ of 60S and 72 ℃ of 90S), 72 ℃ of prolongation 10min.The PCR product detects with 1.5% agarose gel electrophoresis: allelotrope 1 (homozygous genotype CC) band is 783 and 498bp, and allelotrope 2 (another homozygous genotype TT) band is 783 and 324bp; Heterozygous genes type (CT) band is 783,498 and 324bp.To Chinese local variety plum mountain pig and peaceful pig, external (abroad) kind Large White, landrace and duroc, and Large White * plum mountain pig hybridized pig detects, its genotype and gene frequency see Table 1.
Table 1 different varieties pig MC3R genotype and gene frequency
Genotype frequency (%) gene frequency (%)
The kind sample number
CC CT TT C T plum mountain pig (Meishan) 12 100 100 peaceful pig (Qingping) 14 100 100 Large Whites (Large White) 8 100 100 Landraces (Landrace) 10 100 100 durocs (Duroc) 8 100 .100 Large White * plum mountain pig 10 100 50 50
Embodiment 3
Whether relevant in order to determine MC3R gene SNP polymorphism with the pig phenotypic difference, select 136 Large Whites * plum mountain pig F
2In generation, is as test materials, adopt embodiment 2 described Bi-PAPS methods to carry out polymorphism scanning, MC3R gene C C genotype observation number is 105 (genotype frequency is 0.78), and the CT genotype is 9 (genotype frequency is 0.06), and the TT genotype is 22 (genotype frequency is 0.16).And adopt the SAS statistical software, to 136 Da Bai * Mei Shan F
2Carried out test of significance and multiple comparisons for different genotype carcass trait and meat proterties, preliminary study result shows does not have significant difference (p＞0.05 sees Table 2) between each characteristic index of MC3R gene different genotype.
2 MC3R CC CT TT 3.11±0.07 2.86±0.25 2.86±0.166-7 2.45±0.06 2.25±0.17 2.30±0.17 1.81±0.05 1.60±0.21 1.71±0.14 1.52±0.05 1.27±0.20 1.48±0.16 1.61±0.04 1.43±0.15 1.51±0.11 30.11±0.49 29.23±1.96 29.74±1.10 19.05±0.44 18.04±1.77 19.20±1.27 57.85±0.37 58.17±1.40 56.60±0.89pH 6.34±0.02 6.25±.102 6.33±0.06pH 6.42±0.02 6.46±0.06 6.41±0.03pH 6.45±0.02 6.43±0.05 6.45±0.04 91.29±0.55 87.36±3.77 88.62±2.25 21.81±0.35 22.72±1.24 23.32±1.26 20.51±0.14 20.72±0.25 21.05±0.41 2.46±0.07 2.28±0.11 2.28±0.133
Sequence table Organization Applicant----------------------
Street: Lion Rock street
City: Wuhan
State: Hubei Province
Country: China
PostalCode：430070
PhoneNumber：027-87282038
FaxNumber：027-87397735
EmailAddress：zhanghb @ mail.hzau.edu.cn＜110〉OrganizationName：Application Project-------------------＜120〉Title：-3＜130〉AppFileReference：＜140〉CurrentAppNumber：＜141〉CurrentFilingDate：2002-09-04Sequence--------＜213〉OrganismName： ( Sus Scroft )＜400〉PreSequenceString：1gccttctgtg agcaggtctt catcaagccc gaagtcttcc tggctctggg catcctcagc 60ctgctggaga acgtgctggt catcctggcc gtggccagga acggcaacct gcactcgccc 120atgtacctct tcctctgcag cctggccgtg gccgacctgc tggtgagcgt gtccaacgcc 180ctggagacca tcatgatcgc cgtggtcaac agcgacgccc tgaccttcga ggaccagttc 240gtccagcaca tggacaacgt cttcgactcc atgatctgca tctcgctggt ggcctccatc 300tgcaacctct tggccatcgc cgtggacagg tacgtcacca tcttctacgc gctgcgctac 360cacagcatca tgaccgtgcg gaaggcgggg gccctgatcg cggccatctg ggtgtgctgc 420ggcgtctgcg gcgtggtctt catcgtctac tccgagagca agatggtcat cgtgtgcctc 480gtcgtcatct tcttcgccat gctgctcctc atgggcaccc tctacgtgca catgtttctc 540ttcgcccggc tgcacgtcca gcgcatcgcc gcgctgccgc ccgccgacgg ggggcccccc 600ccgcagcgct cgtgcctgaa gggggccgtg accatctccc tcctgctggg ggtcttcatc 660ttctgctggg cccccttctt tctccacctg gttctcatca tcacctgccc cacccacccc 720tactgcatct gctacaccgc ccacttcaac acctacctgg tcctcatcat gtgcaactcc 780gtc 783＜212〉Type：DNA＜211〉Length：783
SequenceName：SEQ?ID?No..1
SequenceDescription：Feature-------Sequence：SEQ?ID?No..1：<221>FeatureKey：exon<222>LocationFrom：1<222>LocationTo：783
Other?Information：
CDSJoin：NoSequence---------＜213〉OrganismName： ( Sus scrofa )＜400〉PreSequenceString：2gccttctgtg agcaggtctt catcaagccc gaagtcttcc tggctctggg catcctcagc 60ctgctggaga acgtgctggt catcctggcc gtggccagga acggcaacct gcactcgccc 120atgtacctct tcctctgcag cctggccgtg gccgacctgc tggtgagcgt gtccaacgcc 180ctggagacca tcatgatcgc cgtggtcaac agcgacgccc tgaccttcga ggaccagttc 240gtccagcaca tggacaacgt cttcgactcc atgatctgca tctcgctggt ggcctccatc 300tgcaacctct tggccatcgc cgtggacagg tacgtcacca tcttctacgc gctgcgctac 360cacagcatca tgaccgtgcg gaaggcgggg gccctgatcg cggccatctg ggtgtgctgc 420ggcgtctgcg gcgtggtctt catcgtctac tccgagagca agatggtcat cgtgtgcctt 480gtcgtcatct tcttcgccat gctgctcctc atgggcaccc tctacgtgca catgtttctc 540ttcgcccggc tgcacgtcca gcgcatcgcc gcgctgccgc ccgccgacgg ggggcccccc 600ccgcagcgct cgtgcctgaa gggggccgtg accatctccc tcctgctggg ggtcttcatc 660ttctgctggg cccccttctt tctccacctg gttctcatca tcacctgccc cacccacccc 720tactgcatct gctacaccgc ccacttcaac acctacctgg tcctcatcat gtgcaactcc 780gtc 783＜212〉Type：DNA＜211〉Length：783
SequenceName：SEQ?ID?No..2
SequenceDescription：Feature-------Sequence：SEQ?ID?No..2：<221>FeatureKey：exon<222>LocationFrom：1<222>LocationTo：783
Other?Information：
CDSJoin:NoSequence--------＜213〉OrganismName: pig (Sus Scroft)＜400〉PreSequenceString:3gccttctgtg agcaggtcttc 21＜212〉Type:DNA＜211〉Length:21
SequenceName：SEQ?ID?No..3
SequenceDescription：Feature--------Sequence：SEQ?ID?No..3：<221>FeatureKey：exon<222>LocationFrom：1<222>LocationTo：21
Other?Information：
CDSJoin:NoSequence--------＜213〉OrganismName: pig (Sus Scroft)＜400〉PreSequenceString:4gacggagttg cacatgatga g 21＜212〉Type:DNA＜211〉Length:21
SequenceName：SEQ?ID?No.4
SequenceDescription：Feature--------Sequence：SEQ?ID?No.4：<221>FeatureKey：exon<222>LocationFrom：1<222>LocationTo：21
Other?Information：
CDSJoin:NoSequence--------＜213〉OrganismName: pig (Sus Scroft)＜400〉PreSequenceString:5aagatggtca tcgtgtgcct t 21＜212〉Type:DNA＜211〉Length:21
SequenceName：SEQ?ID?No.5
SequenceDescription：Feature-------Sequence：SEQ?ID?No.5：<221>FeatureKey：exon<222>LocationFrom：1<222>LocationTo：21
Other?Information：
CDSJoin:NoSequence--------＜213〉OrganismName: pig (Sus Scroft)＜400〉PreSequenceString:6ggcgaagaag atgacgacg 19＜212〉Type:DNA＜211〉Length:19
SequenceName：SEQ?ID?No.6
SequenceDescription：Feature-------Sequence：SEQ?ID?No.6：<221>FeatureKey：exon<222>LocationFrom：1<222>LocationTo：19
Other?Information：
CDSJoin：No
Claims (5)
1, pig melanocortin-3 acceptor (MC3R) gene, its coding nucleotide sequence is shown in sequence table SEQ ID No.1 and SEQ ID No.2.
2,, it is characterized in that 480 in the Nucleotide of MC3R gene PCR amplification has a single nucleotide polymorphism (SNP) according to right 1 described dna sequence dna.
3 methods as claimed in claim 1 or 2, its feature is according to following steps: extract DNA from the pig blood genome, according to people and mouse MC3R gene conserved regions design primer, pcr amplification, PCR product purification and directly order-checking are by the nucleotide sequence of sequence comparing analysis acquisition shown in sequence table SEQ ID No.1 and sequence table SEQ ID No.2.
4, method according to claim 3 is characterized in that: the method that is used for the detection of pig MC3R gene SNP somatotype is to adopt the two-way pcr amplification of special allelotrope (Bi-PASA) step.
5, method as claimed in claim 4 is characterized in that: the dna sequence dna of four kinds of primers is respectively shown in sequence table SEQ ID No.3, SEQ ID No.4, SEQ ID No.5, SEQ ID No.6:
Sequence table SEQ ID No.3:
F
1：5’-GCC?TTC?TGT?GAG?CAG?GTC?TTC-3’
Sequence table SEQ ID No.4:
R
1：5’-GAC?GGA?GTT?GCA?CAT?GAT?GAG-3’
Sequence table SEQ ID No.5:
F
2：5’-AAG?ATG?GTC?ATC?GTG?TGC?CTT-3’
Sequence table SEQ ID No.6:
R
2：5’-GGC?GAA?GAA?GAT?GAC?GAC?G-3’
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021390010A CN1480532A (en) | 2002-09-04 | 2002-09-04 | Gene of cortexin-3 receptor of pig melanin and method for detecting polymorphism of mononucleotide |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021390010A CN1480532A (en) | 2002-09-04 | 2002-09-04 | Gene of cortexin-3 receptor of pig melanin and method for detecting polymorphism of mononucleotide |
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|---|---|---|---|
| CNA021390010A Pending CN1480532A (en) | 2002-09-04 | 2002-09-04 | Gene of cortexin-3 receptor of pig melanin and method for detecting polymorphism of mononucleotide |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1300313C (en) * | 2004-07-16 | 2007-02-14 | 华中农业大学 | Clone of gene MAC30 related to pig backfat thickness and its use in mark-assisted selection |
| CN1333076C (en) * | 2005-01-12 | 2007-08-22 | 华中农业大学 | Pig muscle enolase ENO3 and its use as genetic marker of pig production trait |
| CN101894216A (en) * | 2010-07-16 | 2010-11-24 | 西安电子科技大学 | Method of discovering SNP group related to complex disease from SNP information |
| CN109022593A (en) * | 2018-08-28 | 2018-12-18 | 扬州大学 | The method that the assisted Selection of liver goose abdominal fat weight and carcass weight marks and utilizes molecular marker assisted selection |
| CN111893196A (en) * | 2020-09-16 | 2020-11-06 | 江苏农牧科技职业学院 | MC4R gene molecular marker related to Sujiang pig production traits and preparation method and application thereof |
-
2002
- 2002-09-04 CN CNA021390010A patent/CN1480532A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1300313C (en) * | 2004-07-16 | 2007-02-14 | 华中农业大学 | Clone of gene MAC30 related to pig backfat thickness and its use in mark-assisted selection |
| CN1333076C (en) * | 2005-01-12 | 2007-08-22 | 华中农业大学 | Pig muscle enolase ENO3 and its use as genetic marker of pig production trait |
| CN101894216A (en) * | 2010-07-16 | 2010-11-24 | 西安电子科技大学 | Method of discovering SNP group related to complex disease from SNP information |
| CN101894216B (en) * | 2010-07-16 | 2012-09-05 | 西安电子科技大学 | Method of discovering SNP group related to complex disease from SNP information |
| CN109022593A (en) * | 2018-08-28 | 2018-12-18 | 扬州大学 | The method that the assisted Selection of liver goose abdominal fat weight and carcass weight marks and utilizes molecular marker assisted selection |
| CN109022593B (en) * | 2018-08-28 | 2021-07-16 | 扬州大学 | Auxiliary selection marker for abdominal fat weight and carcass weight of liver goose and method for auxiliary selection by using molecular marker |
| CN111893196A (en) * | 2020-09-16 | 2020-11-06 | 江苏农牧科技职业学院 | MC4R gene molecular marker related to Sujiang pig production traits and preparation method and application thereof |
| CN111893196B (en) * | 2020-09-16 | 2023-03-14 | 江苏农牧科技职业学院 | MC4R gene molecular marker related to Sujiang pig production traits and preparation method and application thereof |
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