CN1465223A - Substratum formula of cultigen for Agrarisis blazei Murril - Google Patents
Substratum formula of cultigen for Agrarisis blazei Murril Download PDFInfo
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- CN1465223A CN1465223A CNA02144661XA CN02144661A CN1465223A CN 1465223 A CN1465223 A CN 1465223A CN A02144661X A CNA02144661X A CN A02144661XA CN 02144661 A CN02144661 A CN 02144661A CN 1465223 A CN1465223 A CN 1465223A
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Abstract
The present invention belongs to the field of edible fungus cultivation technology, and relates to a culture medium formula of culture seed of Agrarisis blazei Murril and its preparation method. Said formula is composed of (wt%) 85-90% of wheat groat, 9-12% of broad-leaf wood saw dust, 0.2-2% of cane sugar, 0.2-1% of gypsum and 0.05-0.4% of urea, and its preparation method includes the steps of soaking, cooking, cooling, stirring, bottling, cleaning, sealing, sterilizing, cooling, inoculating and culturing, etc.
Description
Technical field: the invention belongs to the fungus growing technique field, relate to culture medium of edible fungus, specifically a kind of Agaricus blazei Murrill cultivated species culture medium prescription and preparation method.
Background technology: the quality of Agaricus blazei Murrill cultivation quality has direct relation with the quality of cultivated species.Medium technology the edible mushroom of the relationship between quality that solves cultivation and cultivated species is widely used.The culture technique of relevant Agaricus blazei Murrill has many reports in some documents.In " screening of Agaricus blazei Murrill mother culture media " (Zhang Pinghua, edible mushroom, 2001) mother culture media is screened; " research of Agaricus blazei Murrill planting technique " (the river branch and, Zhu Dan, the edible mushroom journal, 1997) told about and a kind ofly added the cultivated species that calcium carbonate is medium with wheat, its culture medium prescription is: wheat 98%, CaCO
32%.
Summary of the invention: a kind of inoculation back mycelia material feeding is fast, growth is rapid, after planting returns the fast way of bacterium by seeking in the present invention, and purpose provides a kind of Agaricus blazei Murrill cultivated species culture medium prescription and preparation method.
Medium of the present invention and preparation method are described in detail in detail below.
Cultivated species culture medium prescription of the present invention comprises primary raw material, filler, carbon and nitrogenous source, buffer.Described primary raw material is a wheat, and wheat berry provides the main nutrient matter of mycelial growth, also can be described as the carbon and the nitrogenous source of mycelial growth.Filler is based on the broad-leaved wood chip, and the main effect of broad-leaved wood chip is to fill the space, and next provides the part carbon source.Here can not replace the broad-leaved wood chip with the needle wood chip.Carbon and nitrogenous source can be sugar and urea, and sugar provides the carbon source of easy utilization, and urea provides the nitrogenous source of easy utilization.Buffer is a gypsum, and gypsum provides Ca and plays cushioning effect.Its concrete prescription is by weight percentage: primary raw material wheat berry 85～90%, broad-leaved wood chip 9～12%, sucrose 0.2～2%, gypsum 0.2～1%, urea 0.05～0.4%.
The immersion of manufacturing process primary raw material wheat of the present invention, boiling, cooling, spice, bottling, clear Xian, seal, sterilize, cool off, inoculation, cultivation, bacterial classification grow up to.The concrete operations main points are as follows:
Soak.At first the primary raw material wheat was soaked 12～24 hours.Before immersion, add a small amount of lime, regulate pH value to 8-12.Add a small amount of lime, can remove the assorted bacterium of part, can shorten digestion time after suctioning moisture.
Boiling.The primary raw material wheat that soaked with pot or other boiling apparatus boiling, generally also needs to boil 10～15 fens after boiling again, and pulls out when a small amount of cracking is arranged.
Spice.Elder generation is with sugar and the urea water dissolves the back and broad-leaved wood chip, gypsum are puddled evenly, evenly puddles then in the good primary raw material wheat of cooling, and water content is advisable about 65～70%.
Bottling.Material after mixing is packed in the bottle.Bottling wants degree of tightness appropriateness, charge to be about about 4/5 of bottle capacity, and charge level will be put down, and will prick an aperture with thin stick in the middle of the bottle, increases permeability.
Seal.Seal bottleneck with polypropylene screen.Bottleneck is tight with the leather sheath envelope, requires the degree of tightness appropriateness, and operation when being convenient to inoculate will prevent that simultaneously assorted bacterium from entering, and the surface will be put down, the fold of having tried not.
Sterilization.Sterilize with high-pressure sterilizing pot.During autoclaving, sterilization is wanted thoroughly, when pressure is raised to 0.5 MPa for the first time, notes getting rid of the cold air in the high-pressure sterilizing pot, prevents " the false pressure " phenomenon; When pressure reaches 0.147 MPa, pick up counting, kept 1.5～2 hours.When high-pressure sterilizing pot pressure reached 0.147 MPa, temperature reached the sterilization requirement.
Inoculation.Begin inoculation when being cooled to below 30 ℃, a general original seed can connect the 3-4 bottle, strict control seeded process, the assorted bacterium of as far as possible protecting from infection.
Cultivate.Cultivation is carried out in culturing room, and culturing room requires dry, lucifuge, and ventilation is good, and the control temperature was generally cultivated 15-25 days under 25 ℃ of conditions between 22-28 ℃, and mycelia just can be covered with bottle.
Manufacturing process should be noted following problem: 1) moisture is excessive, and the charging tension causes that gas permeability is poor, and it is very fast that mycelia shows as surperficial material feeding, but not down material feeding, after several days, the ponding layer appears in the bottom in the bottle; 2) urea amount is big, produces free ammonia, suppresses mycelial growth, if urea is an amount of, the nutrient abundance can make mycelial growth fast, and mycelia is sturdy; 3) sugaring amount is big, the easy infection bacterium of mixing; 4) sterilization is not thorough, if grow the bacterium of mixing from bottle bottom or centre earlier,, then may be to enter assorted bacterium when connecing bacterium if grow assorted bacterium earlier from the surface then because sterilization does not thoroughly cause, if assorted bacterium appears in the late stage of culture surface, then may be culturing room's environment pollution.
Find out that from above several reasons being not only the medium composition influences the growth situation, preparation method and process have influence on the cultivation of cultivated species equally.
The present invention inoculates the back and compares with conventional wheat kind, and mycelia is pure white, sturdy, even up and down, and the media surface aerial hyphae is vigorous, and the mycelia material feeding is fast.Under 26 ± 2 ℃ of conditions, just can cover with bottle in about 15～20 days.The bacterial classification of making is used for the cultivation of a bed formula, and it is fast after planting to return bacterium, and mycelial growth rate is fast, can effectively prevent living contaminants, and pollution rate is no more than 5%, the fruiting time comparable common wheat kind (wheat 98%, CaCO
32%) carries about the last week.
Embodiment
Carried out the making of cultivated species in 2002 in the following manner:
Culture medium prescription, by weight percentage, wheat berry 85%, broad-leaved wood chip 12%, sucrose 0.2～2%, gypsum 0.2～1%, urea 0.05～0.4%.
Cultivated species medium manufacturing process is as follows.
1) soaks: at first wheat was soaked about 12 hours, and before immersion, added a small amount of lime, regulate pH value to 9, remove the assorted bacterium of part, shorten digestion time after suctioning moisture.
2) boiling: the wheat that soaked is placed on boiling in the pot, boiled again after boiling 10 fens, pull out when a small amount of cracking is arranged.
3) spice: earlier sugared water is dissolved the back and puddle evenly with broad-leaved wood chip, gypsum, evenly puddle then in the good wheat of cooling, water content is about 65%.
4) bottling: be divided in the 750ml Cans of wash clean, charging degree of tightness appropriateness, charge is about about 4/5 of bottle capacity, and charge level is flat with hand, pricks an aperture with thick 1cm thin stick in the middle of the bottle, increases permeability.
5) seal: seal with polypropylene screen, the leather sheath that is cut into bicycle tube when sealing is sealed, and requires the degree of tightness appropriateness, and operation when being convenient to inoculate prevents that assorted bacterium from entering, and the surface will be put down, the fold of having tried not.
6) sterilization: sterilize with high-pressure sterilizing pot.During autoclaving, when pressure is raised to 0.5 MPa for the first time, get rid of the cold air in the high-pressure sterilizing pot, when pressure reaches 0.147 MPa, pick up counting, kept 2 hours.
7) inoculation: begin inoculation when being cooled to below 30 ℃, an original seed connects 4 bottles, and strict control seeded process is to prevent bacteria infection.
8) cultivate: culturing room's drying, lucifuge, ventilation is good, cultivates 18 days under 25 ± 1 ℃ of conditions, and mycelia is covered with bottle.Mycelia is pure white, sturdy, even up and down, and the growth of media surface aerial hyphae is vigorous.
Claims (4)
1, a kind of Agaricus blazei Murrill cultivated species medium is characterized in that prescription comprises primary raw material, filler, carbon and nitrogenous source, buffer; Described primary raw material is a wheat, filler is based on the broad-leaved wood chip, the main effect of broad-leaved wood chip is to fill the space, next provides the part carbon source, carbon and nitrogenous source can be sugar and urea, and sugar provides the carbon source of easy utilization, and urea provides the nitrogenous source of easy utilization, buffer is a gypsum, and gypsum provides Ca and plays cushioning effect; Its concrete prescription is by weight percentage: primary raw material wheat berry 85～90%, broad-leaved wood chip 9～12%, sucrose 0.2～2%, gypsum 0.2～1%, urea 0.05～0.4%.
2, Agaricus blazei Murrill cultivated species medium preparation method according to claim 1 is characterized in that through immersion, boiling, spice, bottles, seals, sterilizes, inoculates, cultivates plurality of processes; 1) soaks, add a small amount of lime before the immersion, regulate pH value, the primary raw material wheat was soaked 12～24 hours to 8-12; 2) boiling, the primary raw material wheat that soaked with pot or other boiling apparatus boiling, boiled after boiling 10～15 fens again, pulled out when a small amount of cracking is arranged; 3) spice dissolves the back with sugar and urea water and puddles evenly with broad-leaved wood chip, gypsum, evenly puddles then in the good primary raw material wheat of cooling; 4) bottling, the material after mixing is packed in the bottle, and degree of tightness appropriateness, charge be 4/5 of bottle capacity; 5) seal, seal bottleneck with polypropylene screen, bottleneck is tight with the leather sheath envelope, the degree of tightness appropriateness; 6) the high-pressure sterilizing pot autoclaving is used in sterilization, and pressure is in 0.147～0.5 MPa; 7) inoculation begins inoculation when being cooled to below 30 ℃, an original seed can connect the 3-4 bottle; 8) cultivate,, carry out under the good condition of ventilation in dry, lucifuge.
3, Agaricus blazei Murrill cultivated species medium preparation method according to claim 2, it is characterized in that spice after water content 65～70%; The when filling with substance charge level will be put down, and pricks an aperture with thin stick in the middle of the bottle, increases permeability; The fold of having tried not will be put down in the surface during envelope bottle; During sterilization, when pressure is raised to 0.5 MPa for the first time, get rid of the cold air in the high-pressure sterilizing pot, when pressure reaches 0.147 MPa, kept 1.5～2 hours; The control temperature is between 22-28 ℃ during cultivation.
4, Agaricus blazei Murrill cultivated species medium preparation method according to claim 3 is characterized in that cultivating 15-25 days under 25 ℃ of conditions.
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| CNB02144661XA CN1179622C (en) | 2002-11-29 | 2002-11-29 | Substratum formula of cultigen for Agrarisis blazei Murril |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101861795A (en) * | 2010-05-10 | 2010-10-20 | 云南农业大学 | Method for promoting propagation of tricholoma matsutake artificially |
| CN101960961A (en) * | 2010-09-09 | 2011-02-02 | 福建省农业科学院土壤肥料研究所 | New high-yield cultivation method for agaricus blazei sowing and earth backing stage management |
| CN102050673A (en) * | 2010-11-11 | 2011-05-11 | 刘晋才 | Preparation method of edible mushroom wood plant mother culture medium and product thereof |
| CN101602624B (en) * | 2009-07-17 | 2011-08-03 | 山西阿林青谷农产品开发有限公司 | Agaricus blazei culture material and preparation method thereof |
| CN102523926A (en) * | 2012-01-09 | 2012-07-04 | 福建省农业科学院土壤肥料研究所 | Method for culturing strain of Agaricus campestris and Agaricus blazei using white loam soil as filter layer |
| CN102786339A (en) * | 2011-05-17 | 2012-11-21 | 吉林农业大学 | Liquid culture medium suitable for tricholoma matsutake fermentation |
| CN103664348A (en) * | 2013-11-15 | 2014-03-26 | 阜南县健生源食用菌开发有限公司 | Agaricus subrufescens cultivation material taking dry straw as raw material and preparation method thereof |
| CN103766131A (en) * | 2012-10-23 | 2014-05-07 | 天一真菌生技农场有限公司 | Tricholoma matsutake cultivating method |
| CN105340572A (en) * | 2015-11-18 | 2016-02-24 | 文山市滇珍菌业有限公司 | Agaricus brasiliensis factory cultivation method |
| CN105453895A (en) * | 2015-12-23 | 2016-04-06 | 嘉兴圣洲生物科技有限公司 | Cultivation technology for Agaricus blazei |
| CN106069193A (en) * | 2016-06-24 | 2016-11-09 | 何建清 | A kind of method that Tricholoma matsutake sporophore is isolated and purified |
| CN108293614A (en) * | 2016-09-27 | 2018-07-20 | 陈晓红 | The method of matsutake edible fungus industrial production |
| CN110226450A (en) * | 2019-06-12 | 2019-09-13 | 肇庆绿健农业科技有限公司 | A kind of breeding method of the brave matsutake of bottle of cultivation |
| CN113475311A (en) * | 2021-08-06 | 2021-10-08 | 贵州省生物研究所 | Agaricus blazei cultivation and planting method |
-
2002
- 2002-11-29 CN CNB02144661XA patent/CN1179622C/en not_active Expired - Fee Related
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101602624B (en) * | 2009-07-17 | 2011-08-03 | 山西阿林青谷农产品开发有限公司 | Agaricus blazei culture material and preparation method thereof |
| CN101861795B (en) * | 2010-05-10 | 2012-06-13 | 云南农业大学 | Method for promoting propagation of tricholoma matsutake artificially |
| CN101861795A (en) * | 2010-05-10 | 2010-10-20 | 云南农业大学 | Method for promoting propagation of tricholoma matsutake artificially |
| CN101960961A (en) * | 2010-09-09 | 2011-02-02 | 福建省农业科学院土壤肥料研究所 | New high-yield cultivation method for agaricus blazei sowing and earth backing stage management |
| CN102050673A (en) * | 2010-11-11 | 2011-05-11 | 刘晋才 | Preparation method of edible mushroom wood plant mother culture medium and product thereof |
| CN102050673B (en) * | 2010-11-11 | 2013-06-19 | 刘晋才 | Preparation method of edible mushroom wood plant mother culture medium and product thereof |
| CN102786339A (en) * | 2011-05-17 | 2012-11-21 | 吉林农业大学 | Liquid culture medium suitable for tricholoma matsutake fermentation |
| CN102786339B (en) * | 2011-05-17 | 2014-04-09 | 吉林农业大学 | Liquid culture medium suitable for tricholoma matsutake fermentation |
| CN102523926A (en) * | 2012-01-09 | 2012-07-04 | 福建省农业科学院土壤肥料研究所 | Method for culturing strain of Agaricus campestris and Agaricus blazei using white loam soil as filter layer |
| CN103766131A (en) * | 2012-10-23 | 2014-05-07 | 天一真菌生技农场有限公司 | Tricholoma matsutake cultivating method |
| CN103664348A (en) * | 2013-11-15 | 2014-03-26 | 阜南县健生源食用菌开发有限公司 | Agaricus subrufescens cultivation material taking dry straw as raw material and preparation method thereof |
| CN105340572A (en) * | 2015-11-18 | 2016-02-24 | 文山市滇珍菌业有限公司 | Agaricus brasiliensis factory cultivation method |
| CN105340572B (en) * | 2015-11-18 | 2018-02-13 | 文山市滇珍菌业有限公司 | A kind of Agricus blazei industrial planting method |
| CN105453895A (en) * | 2015-12-23 | 2016-04-06 | 嘉兴圣洲生物科技有限公司 | Cultivation technology for Agaricus blazei |
| CN106069193A (en) * | 2016-06-24 | 2016-11-09 | 何建清 | A kind of method that Tricholoma matsutake sporophore is isolated and purified |
| CN108293614A (en) * | 2016-09-27 | 2018-07-20 | 陈晓红 | The method of matsutake edible fungus industrial production |
| CN110226450A (en) * | 2019-06-12 | 2019-09-13 | 肇庆绿健农业科技有限公司 | A kind of breeding method of the brave matsutake of bottle of cultivation |
| CN110226450B (en) * | 2019-06-12 | 2021-12-28 | 向国忠 | Cultivation method of bottle-cultivated tiger tricholoma matsutake |
| CN113475311A (en) * | 2021-08-06 | 2021-10-08 | 贵州省生物研究所 | Agaricus blazei cultivation and planting method |
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| CN1179622C (en) | 2004-12-15 |
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