CN1460852A - Multifunctional blood detection chip - Google Patents
Multifunctional blood detection chip Download PDFInfo
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- CN1460852A CN1460852A CN 03137571 CN03137571A CN1460852A CN 1460852 A CN1460852 A CN 1460852A CN 03137571 CN03137571 CN 03137571 CN 03137571 A CN03137571 A CN 03137571A CN 1460852 A CN1460852 A CN 1460852A
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- 239000008280 blood Substances 0.000 title claims abstract description 45
- 210000004369 blood Anatomy 0.000 title claims abstract description 45
- 238000001514 detection method Methods 0.000 title abstract description 3
- 108090000790 Enzymes Proteins 0.000 claims abstract description 37
- 102000004190 Enzymes Human genes 0.000 claims abstract description 37
- 239000000758 substrate Substances 0.000 claims description 40
- 238000012360 testing method Methods 0.000 claims description 18
- 239000004020 conductor Substances 0.000 claims description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 abstract description 14
- 235000012000 cholesterol Nutrition 0.000 abstract description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 2
- 239000008103 glucose Substances 0.000 abstract description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract 1
- 238000005070 sampling Methods 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 32
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 12
- 239000003153 chemical reaction reagent Substances 0.000 description 11
- 235000014655 lactic acid Nutrition 0.000 description 6
- 239000004310 lactic acid Substances 0.000 description 6
- 235000000346 sugar Nutrition 0.000 description 6
- 239000000463 material Substances 0.000 description 5
- 206010012601 diabetes mellitus Diseases 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229920000515 polycarbonate Polymers 0.000 description 3
- 239000004417 polycarbonate Substances 0.000 description 3
- WHBMMWSBFZVSSR-UHFFFAOYSA-N 3-hydroxybutyric acid Chemical compound CC(O)CC(O)=O WHBMMWSBFZVSSR-UHFFFAOYSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 208000010444 Acidosis Diseases 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000004737 colorimetric analysis Methods 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 238000006479 redox reaction Methods 0.000 description 2
- 108010089254 Cholesterol oxidase Proteins 0.000 description 1
- 208000028399 Critical Illness Diseases 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 108010073450 Lactate 2-monooxygenase Proteins 0.000 description 1
- 241000446313 Lamella Species 0.000 description 1
- 206010027417 Metabolic acidosis Diseases 0.000 description 1
- 102100021674 Protein scribble homolog Human genes 0.000 description 1
- 101710169810 Protein scribble homolog Proteins 0.000 description 1
- 206010040070 Septic Shock Diseases 0.000 description 1
- 102000000019 Sterol Esterase Human genes 0.000 description 1
- 108010055297 Sterol Esterase Proteins 0.000 description 1
- WGKMWBIFNQLOKM-UHFFFAOYSA-N [O].[Cl] Chemical compound [O].[Cl] WGKMWBIFNQLOKM-UHFFFAOYSA-N 0.000 description 1
- WDJHALXBUFZDSR-UHFFFAOYSA-M acetoacetate Chemical compound CC(=O)CC([O-])=O WDJHALXBUFZDSR-UHFFFAOYSA-M 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002651 drug therapy Methods 0.000 description 1
- 238000003487 electrochemical reaction Methods 0.000 description 1
- 125000004494 ethyl ester group Chemical group 0.000 description 1
- 201000005577 familial hyperlipidemia Diseases 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000011229 interlayer Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- -1 potassium ferricyanide Chemical compound 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000003716 rejuvenation Effects 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000036303 septic shock Effects 0.000 description 1
- 238000004544 sputter deposition Methods 0.000 description 1
- 230000000287 tissue oxygenation Effects 0.000 description 1
- 208000003663 ventricular fibrillation Diseases 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The multifunctional blood detection chip capable of simultaneously detecting the concentrations of four different component of glucose and cholesterol, etc. includes upper layer base plate, lower layer base plate, sampling hole, exhausting hole and intermediate sandwich with cruciform groove between upper layer base plate and lower layer base plate, on the described lower base plate four pairs of enzyme electrodes which are cross-arranged are set, every pair of enzyme electrodes is respectively connected with every pair of wires, the tail end of the wire is equipped with wiring terminal.
Description
Technical field
The present invention relates to a kind of biology sensor, relate in particular to a kind of structural design of multi-functional blood testing chip, belong to the biosensor technology field.
Background technology
At present, the whole world has 100,012,000 diabetics, and developed countries onset diabetes rate has reached 2%-10%, domesticly should the disease population size have also surpassed 4,000 ten thousand people, accounts for about 4% of total population. and diabetes have become human the 3rd dead killer.At present onset diabetes rate every year is with 10% speed increase, but recall rate has only 20%, that is to say, has 80% patient to get diabetes but do not know, and these people overwhelming majority are early stage patients, because of not knowing that the state of an illness very easily increases the weight of.In addition, along with variations such as diet, habits and customs, working pressures, the people who suffers from high fat of blood is more and more.China is populous, and hyperlipemia has reached 2.16 hundred million more than at present, and the trend of rejuvenation is gradually arranged.Among this crowd, the people who suffers from angiocardiopathies such as high fat of blood, heart disease is quite a lot of, accounts for more than 70% of this age level more than 40 years old.One of effective means that solves hyperglycaemia and high fat of blood is the frequent test to compositions such as blood sugar in the patient blood and blood fat, and auxiliary with drug therapy and diet control.Ketoboidies is the fatty product that decomposes in a large number and fail abundant oxidation, comprises acetoacetate, acetone and beta-hydroxybutyric acid.If human body produces a large amount of ketoboidies at short notice and piles up in vivo, will cause metabolic acidosis, severe patient is with threat to life, and therefore the periodic monitoring ketoboidies is extremely important in case of necessity.The acidaemia that lactate can rapider reflection than acidity or oxygen chlorine saturation degree produces because of tissue oxygenation is on the low side, lactic acid content is to judge the various after one's own heart important indicators of the criticality of the state of an illness such as burst infraction, ventricular fibrillation, septic shock of situation of being critically ill.
In the prior art in the detection by quantitative blood method of composition such as blood sugar mainly contain colourimetry and electrochemical process.Utilize the colourimetry precision of optical reflection principle lower, light path system and apparatus structure more complicated.And the biochemical sensor that utilizes enzyme electrode to make is easy to use, and the measurement sensitivity height can obtain test result fast.The biology sensor of existing measuring blood and cholesterol comes out both at home and abroad at present.China invention invention (invention number: 99108434.9) disclose a kind of biology sensor, this sensor is made up of two pole plates up and down, on one of them pole plate pair of electrodes is arranged, scribble enzyme reagent and the reagent layer that contains electron transmitter on the electrode, but this sensor all can only detect a physiochemical indice, and two leads that link to each other with electrode lay respectively on the different pole plates, and structure is relatively complicated, what electrode processing method adopted is sputtering method, and cost is higher.
In actual applications, often need to measure simultaneously (index) concentration of multiple composition in the blood, have only the large-scale Medical Instruments of the complex and expensive utilized just can accomplish this point at present, testing cost is very high.
Summary of the invention
The purpose of this invention is to provide a kind of multi-functional blood testing chip that can measure many index in the blood simultaneously simple in structure, with low cost, different enzyme reagent is solidificated on the different electrodes of same substrate, utilize this multi-functional blood testing chip can measure blood sugar in the blood simultaneously, cholesterol, lactic acid, ketoboidies or the content of other four kinds of materials in blood.
The objective of the invention is to be achieved through the following technical solutions: a kind of multi-functional blood testing chip, comprise upper strata substrate, lower floor's substrate and annotate sample hole and vent port, it is characterized in that: an intermediate course that has the cruciform groove is set between upper strata substrate and lower floor's substrate, on described lower floor substrate, be provided with four pairs of enzyme electrodes that are cross layout, each joins with couple of conductor respectively to enzyme electrode, and wire end is a connection terminal; Described four pairs of enzyme electrodes are positioned at two substrates up and down and have the space that the intermediate course of cross groove constitutes.
Feature of the present invention also is: described vent port is arranged on the substrate of upper strata, is four vent ports and cross symmetric offset spread, communicates with the cruciform groove of intermediate course; Described notes sample hole is arranged on the substrate of upper strata, and corresponding with the center of cross groove.
Multi-functional blood testing chip provided by the present invention, be that four kinds of different enzyme reagent are solidificated on the different enzyme electrodes of same substrate, therefore can once realize measuring four physiochemical indices such as blood sugar, cholesterol, lactic acid, ketoboidies or other four kinds of physiochemical indices.Simple in structure, cost is low.The cross space that the present invention is positioned at four pairs of enzyme electrodes to have up and down two substrates and intermediate course constitute, blood sample is diffused on four pairs of enzyme electrodes by on-chip notes sample hole, upper strata.Adopt this design can dwindle the volume of chip, reduce blood using amount, only need splash into once bleeding and to be full of whole measured zone; Because four pairs of enzyme electrodes lay respectively on four limits of cruciform groove, do not contact mutually each other, have suppressed influence each other to greatest extent.
Description of drawings
Fig. 1 is one-piece construction synoptic diagram of the present invention (a main cut-open view).
Fig. 2 is the structural representation of upper strata substrate.
Fig. 3 is the structural representation of the intermediate course of band cruciform groove.
Fig. 4 is the structural representation of lower floor's substrate.
Wherein:
1-annotates 2-upper strata, sample hole substrate 3-vent port 4-cross groove 5-intermediate course
6-working electrode 7-is to the substrate 9-of electrode 8-lower floor connection terminal 10 leads
Embodiment
Further specify concrete structure of the present invention and enforcement below in conjunction with accompanying drawing.
Fig. 1 is the structural representation of multi-functional blood testing chip provided by the invention, and this chip comprises upper strata substrate 2, lower floor's substrate 8 and an intermediate course 5 that has cruciform groove 4.Four pairs of enzyme electrodes are arranged on lower floor's substrate 8, and each is made up of electrode 7 working electrode 6 and one enzyme electrode.3, four vent ports of four vent ports are set on the substrate of upper strata are cross symmetric offset spread, corresponding with the position of the cruciform groove of intermediate course respectively.Annotate sample hole 1 and be arranged on the upper strata substrate 2, and corresponding with the center of cross groove.The enzyme and the biological reagent of different types of participation generation redox reaction on every pair of enzyme electrode, have been solidified respectively, because intermediate course 5 is very thin, so in the middle of upper and lower layer substrate, form a criss-cross lamella space, four pairs of enzyme electrodes on lower floor's substrate 8 all are positioned at the cross space that is made of up and down two substrates and intermediate course, and lay respectively on four limits of cruciform groove.Blood sample is diffused on four pairs of enzyme electrodes by on-chip notes sample hole, upper strata 1.Because capillary action blood can be from annotating 1 diffusion of sample hole and being full of whole cross groove, unnecessary air can be discharged from four vent ports 3 of upper strata substrate.Adopt this design can dwindle the volume of chip, reduce blood using amount, only need splash into once bleeding and to be full of whole measured zone; Because four pairs of enzyme electrodes lay respectively on four limits of cruciform groove, do not contact mutually each other, have suppressed influence each other to greatest extent.
The last subtegulum of multi-functional blood testing chip and interlayer can be made by high molecular polymerization materials such as polycarbonate, poly-ethyl esters, and these materials are non-conductive, and are pliable and tough, and certain rigidity and intensity are arranged.Utilize silver-colored paste on lower floor's substrate 8, to form and working electrode 6 and lead 10 that electrode 7 is linked to each other and connection terminal 9, with the working electrode 6 of carbon paste formation rectangular shape with to electrode 7 by the method for serigraphy.Enzyme reagent and electron transfer mediator are solidificated in electrode surface formation biological elements, when applying certain voltage when the working electrode of on-chip four pairs of enzyme electrodes with between to electrode to lower floor, different electrochemical reactions can take place with four pairs of enzyme electrodes respectively in the blood in the cruciform groove, produce electric current at the working electrode of every pair of enzyme electrode with between, and link to each other with the external testing instrument with connection terminal 9 by lead 10 to electrode; These biological elements respectively with blood in material generation redox reactions such as blood sugar, cholesterol, lactic acid, ketoboidies, produce oxidation current, can measure the concentration of materials such as blood sugar in the blood, cholesterol, lactic acid and ketoboidies by the size of measuring electric current.
Embodiment:
With thickness is the polycarbonate plate cut growth 32mm of 0.2mm millimeter, the small pieces of wide 16mm, be used for forming the upper strata substrate 2 and the intermediate course 5 of multi-functional blood testing chip, wherein on upper strata substrate 2, have notes sample hole 1 and four vent ports 3 that diameter is 1mm of a diameter 2mm, these four vent ports are cross arrangement, the position is corresponding with the position of the cruciform groove of intermediate course 5, annotates the center that sample hole 1 is positioned at cross.
Then at the polycarbonate plate cut growth 32mm that with thickness is the 0.5mm millimeter, the small pieces of wide 16mm form lower floor's substrate 8 of multi-functional blood testing chip.Utilize silver-colored paste on lower floor's substrate 8, to form and working electrode 6 and lead 10 that electrode 8 is linked to each other and connection terminal 9 by the method for serigraphy, with the working electrode 6 of carbon paste formation rectangular shape with to electrode 8, the length of electrode and wide 1.8mm and the 1.5mm of being respectively.Wherein the enzyme reagent that solidifies on the working electrode of first pair of enzyme electrode is glucose oxidase, be used for measuring the concentration of glucose in the blood, the enzyme reagent that solidifies on the working electrode of second pair of enzyme electrode is Lactate Oxidase, be used for measuring concentration of lactic acid in the blood, the enzyme reagent that solidifies on the working electrode of the 3rd pair of enzyme electrode is cholesterol oxidase and cholesterol ester hydrolase, be used for measuring the concentration of cholesterol in the blood, the enzyme reagent that solidifies on the working electrode of the 4th pair of enzyme electrode is b-hydroxybutyric dehydrogenase (HBD) and reducibility coenzyme, is used for measuring the concentration of ketoboidies in the blood.Electron transport enzyme medium adopts potassium ferricyanide solution, above enzyme reagent and solution is added cementing agent such as gelatin and is sprayed on the working electrode on the bottom substrate at room temperature dry then 4-8 hour.At last upper strata substrate, the intermediate course and the lower floor substrate that have a cross groove are in the same place with adhesive bond, finish the making of whole multi-functional blood testing chip.
Claims (3)
1. multi-functional blood testing chip, comprise upper strata substrate (2), lower floor's substrate (8) and annotate sample hole (1) and vent port (3), it is characterized in that: an intermediate course (5) that has cruciform groove (4) is set between upper strata substrate and lower floor's substrate, on described lower floor substrate, be provided with four pairs of enzyme electrodes that are cross layout, each joins with couple of conductor (10) respectively to enzyme electrode, and wire end is connection terminal (9); Described four pairs of enzyme electrodes are positioned at two substrates up and down and have the space that the intermediate course of cross groove constitutes.
2. according to the described multi-functional blood testing chip of claim 1, it is characterized in that: described vent port (3) is arranged on the substrate of upper strata, is four vent ports and cross symmetric offset spread, and is corresponding with the position of the cruciform groove of intermediate course.
3. according to claim 1 or 2 described multi-functional blood testing chips, it is characterized in that: described notes sample hole (1) is arranged on the substrate of upper strata, and corresponding with the center of cross groove.
Priority Applications (1)
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CN 03137571 CN1194223C (en) | 2003-06-18 | 2003-06-18 | Multifunctional blood detection chip |
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CN 03137571 CN1194223C (en) | 2003-06-18 | 2003-06-18 | Multifunctional blood detection chip |
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CN1460852A true CN1460852A (en) | 2003-12-10 |
CN1194223C CN1194223C (en) | 2005-03-23 |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102353707A (en) * | 2011-07-08 | 2012-02-15 | 无锡市金坤生物工程有限公司 | Cholesterol molecule blotting membrane sensing electrode and use of the cholesterol molecule blotting membrane sensing electrode in waste oil detection |
CN101650331B (en) * | 2009-08-27 | 2012-09-05 | 无锡爱康生物科技有限公司 | Enzyme bioelectrochemical sensing chip and preparation method thereof |
CN103959051A (en) * | 2011-10-31 | 2014-07-30 | 喜来健迈德斯 | Multi-reaction biosensor |
CN104749228A (en) * | 2013-12-30 | 2015-07-01 | 深圳先进技术研究院 | Test strip and method for detecting blood glucose and blood lipid simultaneously |
CN105241930A (en) * | 2015-09-24 | 2016-01-13 | 浙江大学 | Multiparameter all-solid-state blood analysis sensor |
CN108828043A (en) * | 2018-06-25 | 2018-11-16 | 湖北中医药大学 | A kind of flexibility perspiration sensor and its preparation method and application |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101153875B (en) * | 2006-09-27 | 2011-11-30 | 勤立生物科技股份有限公司 | Sensing member used for detecting total cholesterol in blood sample |
-
2003
- 2003-06-18 CN CN 03137571 patent/CN1194223C/en not_active Expired - Fee Related
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101650331B (en) * | 2009-08-27 | 2012-09-05 | 无锡爱康生物科技有限公司 | Enzyme bioelectrochemical sensing chip and preparation method thereof |
CN102353707A (en) * | 2011-07-08 | 2012-02-15 | 无锡市金坤生物工程有限公司 | Cholesterol molecule blotting membrane sensing electrode and use of the cholesterol molecule blotting membrane sensing electrode in waste oil detection |
CN102353707B (en) * | 2011-07-08 | 2014-02-12 | 无锡市金坤生物工程有限公司 | Cholesterol molecule blotting membrane sensing electrode and use of cholesterol molecule blotting membrane sensing electrode in waste oil detection |
CN103959051A (en) * | 2011-10-31 | 2014-07-30 | 喜来健迈德斯 | Multi-reaction biosensor |
CN104749228A (en) * | 2013-12-30 | 2015-07-01 | 深圳先进技术研究院 | Test strip and method for detecting blood glucose and blood lipid simultaneously |
CN105241930A (en) * | 2015-09-24 | 2016-01-13 | 浙江大学 | Multiparameter all-solid-state blood analysis sensor |
CN108828043A (en) * | 2018-06-25 | 2018-11-16 | 湖北中医药大学 | A kind of flexibility perspiration sensor and its preparation method and application |
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CN1194223C (en) | 2005-03-23 |
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