CN1442476A - Liquid fermentation process of streptomyces flavus and its production equipment - Google Patents
Liquid fermentation process of streptomyces flavus and its production equipment Download PDFInfo
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- CN1442476A CN1442476A CN 03112094 CN03112094A CN1442476A CN 1442476 A CN1442476 A CN 1442476A CN 03112094 CN03112094 CN 03112094 CN 03112094 A CN03112094 A CN 03112094A CN 1442476 A CN1442476 A CN 1442476A
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Abstract
A liquid fermenting process of xanthostreptomycete includes such steps as proportioning raw materials, disinfecting seeding tank at 115-126 deg.C for 30-40 min, inoculating seeds at 26-30 deg.C, fermenting in seeding tank at 28-30 deg.C for 40-48 hr, disinfecting fermentor, inoculating and fermenting. Its fermenting apparatus is composed of screw conveyer, mixing tank, aseptic air maker, seeding tank, fermentor, and discharge valve. Its advantages are high efficiency, less pollution, and simple process.
Description
Technical field
The invention belongs to the streptomyces microflavus fermentation technical field, more specifically to the improvement of the liquid fermentation process and the production unit thereof of streptomyces microflavus.
Background technology
Existing streptomyces microflavus is bred in soil.This bacterial classification in process of growth, can dissociate in a large number have cured in the soil can not be improved the utilization ratio of chemical fertilizer by the nitrogen of crop absorption, phosphorus, potassium in a large number, effectively alleviate because the negative effects such as soil compaction that a large amount of use chemical fertilizer cause.Simultaneously, can generate multiple growth parahormone again, promote plant growth, improve its degeneration-resistant proterties.Streptomyces microflavus can also produce multiple antibiotic, suppresses the germ growth, and this is highly beneficial to eliminating the soil-borne disease bacterium.
But, in the soil streptomyces microflavus quantity of self-sow very little, insignificant.And the domestic artificial production that does not also have this type of bacterial classification does not at present just have corresponding production technique and production unit yet.
Purpose of the present invention just is to overcome above-mentioned shortcoming and defect, provide a kind of can be efficiently mass-produced, the liquid fermentation process and the production unit thereof of sophisticated streptomyces microflavus.
Summary of the invention
In order to achieve the above object, the liquid fermentation process of streptomyces microflavus of the present invention comprises the steps:
(1) being mixed of protein and carbohydrate supplementary material: with protein and carbohydrate supplementary material, water and sodium hydroxide feed material mixing tank, mix while sending, until mixing, the solids concentration that makes supplementary material is 30～35%, and the pH value of supplementary material is 8.5～9.0;
(2) sterilization of seeding tank slack tank and real jar: sterilization slack tank earlier, after real jar of the supplementary material sterilization of packing into, with the superheated vapour sterilization, sterilisation temp is 115～126 ℃, the time is 30～40 minutes;
(3) inoculation: after the real jar of sterilization,, during to 26～30 ℃, press the seed of 1～2% inoculation streptomyces microflavus of supplementary material mass ratio with tube-cooled water cooling material;
(4) seeding tank fermentation: leavening temperature is controlled at 28～30 ℃, the flow that feeds sterile air preceding ten hours be supplementary material volume * (0.4～0.6)/hour, be later on supplementary material volume * (1.1～0.9)/hour, fermentation time is 40～48 hours;
(5) sterilization of fermentor tank slack tank and real jar: sterilization slack tank earlier, after real jar of the supplementary material sterilization of packing into, with the superheated vapour sterilization, sterilisation temp is 115～126 ℃, the time is 30～40 minutes;
(6) inoculation: after the real jar of sterilization,, during to 26～30 ℃, press the seed that 8～12% of supplementary material mass ratio is inoculated the streptomyces microflavus of seeding tank fermentation gained with pipeline water cooling supplementary material;
(7) ferment tank: leavening temperature is controlled at 28～30 ℃, the flow that feeds sterile air preceding ten hours be supplementary material volume * (0.4～0.6)/hour, be later on supplementary material volume * (1.1～0.9)/hour, fermentation time is 40～48 hours;
(8) open bleeder valve discharging packing.
Protein and carbohydrate supplementary material are made up of the soybean cake powder of mass ratio (down together) by 1～5%, 1～5% Semen Maydis powder, 1～3% white sugar, 1～5% wheat bran, 0.1～1% sodium-chlor, 0.1～1.5% lime carbonate and remaining water.Protein that this proportioning is made and the fermentation of carbohydrate supplementary material are very fast, and effect is comparatively desirable.
The production technique of sterile air is: gather air in the upper atmosphere more than the 10M of the face of land, it is carried out coarse filtration remove solid dust, enter gas-holder with the air compression of air compressor machine after with coarse filtration, remove airborne water and oil by gas-liquid separator separates, then air being sent into loft drier further dewaters and cleaningization through cascade filtration, send into heat exchanger again and carry out the constant temperature processing, but then send into the cotton strainer filtration sterilization of constant temperature and pressure autosterilization, but the secondary absolute filter of sending into same constant temperature and pressure autosterilization subsequently filters, detect the quality of sterile air at last with the filter effect determinator, qualified back feeds seeding tank or fermentor tank enters fermentation procedure.The sterile air of this explained hereafter is very clean, and quality is good, can satisfy the requirement of zymotechnique.
Protein and carbohydrate supplementary material are made up of the soybean cake powder of mass ratio (down together) by 2%, 2% Semen Maydis powder, 1% white sugar, 1% wheat bran, 0.3% sodium-chlor, 0.6% lime carbonate and remaining water.Protein that this proportioning is made and the fermentation of carbohydrate supplementary material are fast, and effect is very good.
In operation (3) inoculation, after the real jar of sterilization, during with tube-cooled water cooling supplementary material to 26～30 ℃, press the seed that 2% of supplementary material mass ratio is inoculated streptomyces microflavus; In operation (6) inoculation, after the real jar of sterilization, during with tube-cooled water cooling supplementary material to 26～30 ℃, the ferment seed of streptomyces microflavus of gained of the 10% inoculation seeding tank of press the supplementary material mass ratio.This inoculative proportion is more reasonable, and fermentation is fast, and effect is very good, can not cause the waste of seed again.
This technology at first is delivered into the mixing of materials jar with protein and carbohydrate supplementary material by an accurate measurement worm conveyor (auger).The operational throughput of auger and the valve of water inlet, the valve that advances sodium hydroxide are interlocked by certain volume and mass ratio, and the solids concentration of supplementary material is controlled at 30～35%, and pH value is 8.5～9.0.The PH meter is installed, PH meter and sodium hydroxide volume pump interlocking on the mixing of materials jar.
Reach after the above-mentioned requirements supplementary material and enter seeding tank and carry out liquid fermenting, whole fermentation process is finished by system.
When supplementary material reaches requirement, transmitter is given system with signal feedback, and system sends instruction and gives the charging variable valve, and the charging variable valve is opened, the beginning charging.High level alarm is installed, high level alarm and charging switch-valve interlocking on seeding tank.Be that high level alarm is given system with signal feedback, system directive charging switch-valve moves.The charging switch-valve cuts out automatically when arriving high liquid level, and charging stops.
Begin to enter the real jar disinfecting steps of seeding tank after charging finishes, temperature sensor and pressure transmitter are housed on the seeding tank, system cpu realizes sterilization automatically by the parameter of these two transmitters according to default to seeding tank.System's slack tank disinfectant principle is identical with real jar of sterilization before the charging.System opened the inoculation valves by the open and close of the signal control tube-cooled water of temperature sensor automatically when temperature drops to 28 ℃ after sterilization finished, and inoculated.Enter the seeding tank fermenting process.
The flow that feeds sterile air in the fermenting process preceding ten hours be supplementary material volume * (0.4～0.6)/hour, be later on supplementary material volume * (1.1～0.9)/hour, fermentation time is 40～48 hours.The temperature of whole process supplementary material all is by being controlled at 28-30 ℃.The flow of sterile air and time recorder interlocking, the temperature of supplementary material and steam regulating valve and cooling sea water regulating valve interlocking are operated automatically by system.Automatically turbidity analyser and dissolved-oxygen content analyser are monitored in real time to the fermentation whole process, can analyze automatically to determine the final time of fermentation ends the supplementary material in the jar.When the fermentation termination, system can obtain being sent by automatic turbidity analyser the signal of 4-20mA.Cup obtains sending the ferment tank process that enters of instructing behind this signal.
The operating method of fermentor tank is identical with seeding tank, comprises sterilization, inoculation, the ferment tank of fermentor tank slack tank and real jar.When the fermentation termination, also be the signal of by automatic turbidity analyser the supplementary material in the jar being analyzed and sent the fermentation termination automatically.After system receives this signal, send the instruction of opening bleeder valve.
The liquid fermenting production unit of streptomyces microflavus comprises the worm conveyor (auger) that sodium hydroxide, process water and protein and carbohydrate supplementary material are mixed at least, the mixing of materials jar that is connected with the outlet of worm conveyor, the seeding tank that is connected with the sterile air preparation apparatus with pipeline and mixing of materials jar respectively, the fermentor tank that is connected with the sterile air preparation apparatus with pipeline and seeding tank respectively, the outlet of fermentor tank is connecting bleeder valve, and seeding tank also is connected with steam-pipe with fermentor tank.
The sterile air preparation apparatus comprises the air pump of gathering air in the upper atmosphere more than the 10M of the face of land at least, be connected the coarse filter that air is carried out coarse filtration removal solid dust with the outlet of air pump, the air compressor machine that is connected with coarse filter with pipeline, the gas-holder that is connected with air compressor machine with pipeline, with pipeline and the tank connected gas-liquid separator of gas storage, the loft drier that is connected with gas-liquid separator with pipeline, the secondary filter that is connected with loft drier with pipeline, the heat exchanger that is connected with secondary filter with pipeline, the cotton strainer that is connected with heat exchanger with pipeline, secondary absolute filter that is connected with the cotton strainer with pipeline and the filter effect determinator that is installed in the outlet of secondary absolute filter.
Whole sterile air preparation apparatus adopts full-automatic control.At first adopted the high-altitude gas production, filter by a coarse filter from the air in the atmosphere, the filtering purpose of this grade is to remove airborne solid dust, a differential manometer (PID) is housed at the two ends of coarse filter, system CPU can be controlled the practical function of coarse filter by the 4-20mA signal that PID gives, when pressure alarm sounds, will start the filtration medium treatment system automatically, thus the sterile air quality that control enters fermentation system.
Air admission air compressor machine behind the prefiltration enters gas-holder after the air compression.Pressure detector is housed on the gas-holder, and the robot control system(RCS) of pressure detector and air compressor machine is carried out chain, and promptly pressure surpasses set(ting)value, and air compressor machine can decommission automatically.Because coarse filter only can remove a spot of airborne water and oil, thus the gas of gas-holder need by gas-liquid separator with airborne water oil part from, enter loft drier then.A tasimetry system is housed in the bottom of gas-liquid separator, and when pressure reaches measured value, the bottom valve of gas-liquid separator can be opened automatically, closes after emitting liquid wherein.
Air in the loft drier passes through heat exchanger again through cascade filtration.Temperature in the heat exchanger can be regulated by the transfer valve of water coolant and steam.
Air admission cotton strainer after the processing.The hygrometric system is installed on the cotton strainer.When humidity exceeded standard, system can report to the police automatically, and the automatic starting of media processing system meeting of cotton strainer is to guarantee Air quality.The cotton strainer also is equipped with the thermometric pressure measuring system, and temp measuring system and steam admission internally piloted valve and the steam internally piloted valve of giving vent to anger is associated, and is connected into system central processor, has realized the constant temperature and pressure autosterilization of cotton strainer.
Air then enters the secondary absolute filter.The secondary absolute filter is identical with the cotton strainer, has also realized autosterilization, and its two ends have installed differential manometer additional simultaneously.Be provided with high-low pressure warning dress system mutually by pressure relay and central processing unit, realized real-time monitoring its filtering medium property.
Air had passed through the filter effect determinator that links to each other with cpu earlier before advancing fermentor tank, advance ullage makings amount with real-time monitoring.This filter effect determinator is provided with pollution alarm, can notify air pollution occurrence at once, is convenient to timely contaminated solution problem, avoids enlargement loss, enhances productivity.
Product of the present invention is liquid, i.e. foliage fertilizer.Its available bottled sale of directly dispatching from the factory.To dilute earlier during use, be sprayed on the blade face of crop with utensil again and apply fertilizer.Also can be used for the fermentative production of solid fertilizer as seed.
Advantage of the present invention and positively effect are: technical process is reasonable, the most of automatic controls of production unit, has got rid of the living contaminants situation that manual operation causes, and greatly reduces the tank switching rate; The production efficiency height is particularly suitable for producing in enormous quantities; Can monitor at any time streptomyces microflavus upgrowth situation, the fermented quality height; Production cost is low.It can be widely used in the production in enormous quantities of streptomyces microflavus and foliage fertilizer.
Description of drawings
Fig. 1 is the synoptic diagram of the liquid fermenting production unit of streptomyces microflavus of the present invention.
Embodiment
The liquid fermentation process of 1. 1 kinds of streptomyces microflavus of embodiment.It comprises the steps:
(1) being mixed of protein and carbohydrate supplementary material: with protein and carbohydrate supplementary material, water and sodium hydroxide feed material mixing tank, mix while sending, until mixing, the solids concentration that makes supplementary material is 30%, and the pH value of supplementary material is 9.0;
(2) sterilization of seeding tank slack tank and real jar: sterilization slack tank earlier, after real jar of the supplementary material sterilization of packing into, with the superheated vapour sterilization, sterilisation temp is 115 ℃, the time is 40 minutes;
(3) inoculation: after the real jar of sterilization,, during to 30 ℃, press the seed of 1% inoculation streptomyces microflavus of supplementary material mass ratio with tube-cooled water cooling supplementary material;
(4) seeding tank fermentation: leavening temperature is controlled at 28 ℃, and the flow that feeds sterile air preceding ten hours was supplementary material volume * 0.4/ hour, is later on supplementary material volume * 0.9/ hour, and fermentation time is 40 hours;
(5) sterilization of fermentor tank slack tank and real jar: sterilization slack tank earlier, after real jar of the supplementary material sterilization of packing into, with the superheated vapour sterilization, sterilisation temp is 115 ℃, the time is 40 minutes;
(6) inoculation: after the real jar of sterilization,, during to 30 ℃, press the seed that 8% of supplementary material mass ratio is inoculated the streptomyces microflavus of seeding tank fermentation gained with tube-cooled water cooling material;
(7) ferment tank: leavening temperature is controlled at 28 ℃, and the flow that feeds sterile air preceding ten hours was supplementary material volume * 0.4/ hour, is later on supplementary material volume * 0.9/ hour, and fermentation time is 48 hours;
(8) open the bleeder valve discharging.
Above-mentioned protein and carbohydrate supplementary material are made up of the soybean cake powder of mass ratio (down together) by 5%, 1% Semen Maydis powder, 3% white sugar, 4% wheat bran, 0.1% sodium-chlor, 1.5% lime carbonate and remaining water.
The production technique of sterile air is: gather air in the upper atmosphere more than the 10M of the face of land, it is carried out coarse filtration remove solid dust, enter gas-holder with the air compression of air compressor machine after with coarse filtration, remove airborne water and oil by gas-liquid separator separates, then air being sent into loft drier further dewaters and cleaningization through cascade filtration, send into heat exchanger again and carry out the constant temperature processing, but then send into the cotton strainer filtration sterilization of constant temperature and pressure autosterilization, but the secondary absolute filter of sending into same constant temperature and pressure autosterilization subsequently filters, detect the quality of sterile air at last with the filter effect determinator, qualified back feeds seeding tank or fermentor tank enters fermentation procedure.
The liquid fermentation process of 2. 1 kinds of streptomyces microflavus of embodiment.It comprises the steps:
(1) being mixed of protein and carbohydrate supplementary material: with protein and carbohydrate supplementary material, water and sodium hydroxide feed material mixing tank, mix while sending, until mixing, the solids concentration that makes supplementary material is 33%, and the pH value of supplementary material is 8.7;
(2) sterilization of seeding tank slack tank and real jar: sterilization slack tank earlier, after real jar of the supplementary material sterilization of packing into, with the superheated vapour sterilization, sterilisation temp is 126 ℃, the time is 35 minutes;
(3) inoculation: after the real jar of sterilization,, during to 26 ℃, press the seed of 1.7% inoculation streptomyces microflavus of supplementary material mass ratio with tube-cooled water cooling material;
(4) seeding tank fermentation: leavening temperature is controlled at 29 ℃, and the flow that feeds sterile air preceding ten hours was supplementary material volume * 0.5/ hour, is later on supplementary material volume * 1/ hour, and fermentation time is 42 hours;
(5) sterilization of fermentor tank slack tank and real jar: sterilization slack tank earlier, after real jar of the supplementary material sterilization of packing into, with the superheated vapour sterilization, sterilisation temp is 123 ℃, the time is 32 minutes;
(6) inoculation: after the real jar of sterilization,, during to 26 ℃, press the seed that 10% of supplementary material mass ratio is inoculated the streptomyces microflavus of seeding tank fermentation gained with tube-cooled water cooling material;
(7) ferment tank: leavening temperature is controlled at 29 ℃, and the flow that feeds sterile air preceding ten hours was supplementary material volume * 0.5/ hour is later on supplementary material volume * 1.1/ hour, and fermentation time is 43 hours;
(8) open the bleeder valve discharging.
Above-mentioned protein and carbohydrate supplementary material are made up of the soybean cake powder of mass ratio (down together) by 1%, 5% Semen Maydis powder, 2% white sugar, 5% wheat bran, 1% sodium-chlor, 0.1% lime carbonate and remaining water.
The production technique of sterile air is with embodiment 1.
The liquid fermentation process of 3. 1 kinds of streptomyces microflavus of embodiment.It comprises the steps:
(1) being mixed of protein and carbohydrate supplementary material: with protein and carbohydrate supplementary material, water and sodium hydroxide feed material mixing tank, mix while sending, until mixing, the solids concentration that makes supplementary material is 35%, and the pH value of supplementary material is 8.5;
(2) seeding tank slack tank and real jar of sterilization: sterilization slack tank earlier, after real jar of the supplementary material sterilization of packing into, with the superheated vapour sterilization, sterilisation temp is 120 ℃, the time is 30 minutes;
(3) inoculation: after the real jar of sterilization,, during to 28 ℃, press the seed of 2% inoculation streptomyces microflavus of supplementary material mass ratio with tube-cooled water cooling material;
(4) seeding tank fermentation: leavening temperature is controlled at 30 ℃, and the flow that feeds sterile air preceding ten hours was supplementary material volume * 0.6/ hour, is later on supplementary material volume * 1.1/ hour, and fermentation time is 48 hours;
(5) sterilization of fermentor tank slack tank and real jar: sterilization slack tank earlier, after real jar of the supplementary material sterilization of packing into, with the superheated vapour sterilization, sterilisation temp is 126 ℃, the time is 30 minutes;
(6) inoculation: after the real jar of sterilization,, during to 29 ℃, press the seed that 12% of supplementary material mass ratio is inoculated the streptomyces microflavus of seeding tank fermentation gained with tube-cooled water cooling material;
(7) ferment tank: leavening temperature is controlled at 30 ℃, and the flow that feeds sterile air preceding ten hours was supplementary material volume * 0.6/ hour, is later on supplementary material volume * 1/ hour, and fermentation time is 40 hours;
(8) open the bleeder valve discharging.
Above-mentioned protein and carbohydrate supplementary material are made up of the soybean cake powder of mass ratio (down together) by 2%, 2% Semen Maydis powder, 1% white sugar, 1% wheat bran, 0.3% sodium-chlor, 0.6% lime carbonate and remaining water.The production technique of sterile air is with embodiment 1.
The liquid fermenting production unit of 4. 1 kinds of streptomyces microflavus of embodiment.As shown in Figure 1.Which comprises at least the worm conveyor 1 (auger) that sodium hydroxide, process water and protein and carbohydrate supplementary material are mixed, the mixing of materials jar 2 that is connected with the outlet of worm conveyor 1, the seeding tank 3 that is connected with the sterile air preparation apparatus with pipeline and mixing of materials jar 2, the fermentor tank 4 that is connected with the sterile air preparation apparatus with pipeline and seeding tank 3 respectively respectively.The outlet of fermentor tank 4 is connecting bleeder valve 5, and seeding tank 3 also is connected with steam-pipe with fermentor tank 4.The sterile air preparation apparatus comprises the air pump 6 of gathering air in the upper atmosphere more than the 10M of the face of land at least, be connected the coarse filter 7 that air is carried out coarse filtration removal solid dust with the outlet of air pump 6, the air compressor machine 8 that is connected with coarse filter 7 with pipeline, the gas-holder 9 that is connected with air compressor machine 8 with pipeline, the gas-liquid separator 10 that is connected with gas-holder 9 with pipeline, the loft drier 11 that is connected with gas-liquid separator 10 with pipeline, the secondary filter 12 that is connected with loft drier 11 with pipeline, the heat exchanger 13 that is connected with secondary filter 12 with pipeline, the cotton strainer 14 that is connected with heat exchanger 13 with pipeline, secondary absolute filter 15 that is connected with cotton strainer 14 with pipeline and the filter effect determinator 16 that is installed in 15 outlets of secondary absolute filter.
Claims (7)
1. the liquid fermentation process of a streptomyces microflavus is characterized in that it comprises the steps:
(1) being mixed of protein and carbohydrate supplementary material: with protein and carbohydrate supplementary material, water and sodium hydroxide feed material mixing tank, mix while sending, until mixing, the solids concentration that makes supplementary material is 30～35%, and the pH value of supplementary material is 8.5～9.0;
(2) sterilization of seeding tank slack tank and real jar: sterilization slack tank earlier, after real jar of the supplementary material sterilization of packing into, with the superheated vapour sterilization, sterilisation temp is 115～126 ℃, the time is 30～40 minutes;
(3) inoculation: after the real jar of sterilization,, during to 26～30 ℃, press the seed of 1～2% inoculation streptomyces microflavus of supplementary material mass ratio with tube-cooled water cooling material;
(4) seeding tank fermentation: leavening temperature is controlled at 28～30 ℃, the flow that feeds sterile air preceding ten hours be supplementary material volume * (0.4～0.6)/hour, be later on supplementary material volume * (1.1～0.9)/hour, fermentation time is 40～48 hours;
(5) sterilization of fermentor tank slack tank and real jar: sterilization slack tank earlier, after real jar of the supplementary material sterilization of packing into, with the superheated vapour sterilization, sterilisation temp is 115～126 ℃, the time is 30～40 minutes;
(6) inoculation: after the real jar of sterilization,, during to 26～30 ℃, press the seed that 8～12% of supplementary material mass ratio is inoculated the streptomyces microflavus of seeding tank fermentation gained with tube-cooled water cooling material;
(7) ferment tank: leavening temperature is controlled at 28～30 ℃, the flow that feeds sterile air preceding ten hours be supplementary material volume * (0.4～0.6)/hour, be later on supplementary material volume * (1.1～0.9)/hour, fermentation time is 40～48 hours;
(8) open the bleeder valve discharging.
2. according to the liquid fermentation process of the described streptomyces microflavus of claim 1, it is characterized in that said protein and carbohydrate supplementary material are made up of the soybean cake powder of mass ratio (down together) by 1～5%, 1～5% Semen Maydis powder, 1～3% white sugar, 1～5% wheat bran, 0.1～1% sodium-chlor, 0.1～1.5% lime carbonate and remaining water.
3. according to the liquid fermentation process of claim 1 or 2 described streptomyces microflavus, the production technique that it is characterized in that said sterile air is: gather air in the upper atmosphere more than the 10M of the face of land, it is carried out coarse filtration remove solid dust, enter gas-holder with the air compression of air compressor machine after with coarse filtration, remove airborne water and oil by gas-liquid separator separates, then air being sent into loft drier further dewaters and cleaningization through cascade filtration, send into heat exchanger again and carry out the constant temperature processing, but then send into the cotton strainer filtration sterilization of constant temperature and pressure autosterilization, but the secondary absolute filter of sending into same constant temperature and pressure autosterilization subsequently filters, detect the quality of sterile air at last with the filter effect determinator, qualified back feeds seeding tank or fermentor tank enters fermentation procedure.
4. according to the liquid fermentation process of the described streptomyces microflavus of claim 2, it is characterized in that said protein and carbohydrate supplementary material are made up of the soybean cake powder of mass ratio (down together) by 2%, 2% Semen Maydis powder, 1% white sugar, 1% wheat bran, 0.3% sodium-chlor, 0.6% lime carbonate and remaining water.
5. according to the liquid fermentation process of the described streptomyces microflavus of claim 1, it is characterized in that in said operation (3) inoculation, after the real jar of sterilization, during with tube-cooled water cooling material to 26～30 ℃, press the seed that 2% of supplementary material mass ratio is inoculated streptomyces microflavus; In operation (6) inoculation, after the real jar of sterilization, during with tube-cooled water cooling supplementary material to 26～30 ℃, the ferment seed of streptomyces microflavus of gained of the 10% inoculation seeding tank of press the supplementary material mass ratio.
6. liquid fermenting production unit according to the streptomyces microflavus of the liquid fermentation process of the described streptomyces microflavus of claim 6, it is characterized in which comprises at least with sodium hydroxide, the worm conveyor (auger) that process water and protein and carbohydrate supplementary material are mixed, the mixing of materials jar that is connected with the outlet of worm conveyor, the seeding tank that is connected with the sterile air preparation apparatus with pipeline and mixing of materials jar respectively, the fermentor tank that is connected with the sterile air preparation apparatus with pipeline and seeding tank respectively, the outlet of fermentor tank is connecting bleeder valve, and seeding tank also is connected with steam-pipe with fermentor tank.
7. liquid fermenting production unit according to the described streptomyces microflavus of claim 6, it is characterized in that said sterile air preparation apparatus comprises the air pump of gathering air in the upper atmosphere more than the 10M of the face of land at least, be connected the coarse filter that air is carried out coarse filtration removal solid dust with the outlet of air pump, the air compressor machine that is connected with coarse filter with pipeline, the gas-holder that is connected with air compressor machine with pipeline, with pipeline and the tank connected gas-liquid separator of gas storage, the loft drier that is connected with gas-liquid separator with pipeline, the secondary filter that is connected with loft drier with pipeline, the heat exchanger that is connected with secondary filter with pipeline, the cotton strainer that is connected with heat exchanger with pipeline, secondary absolute filter that is connected with the cotton strainer with pipeline and the filter effect determinator that is installed in the outlet of secondary absolute filter.
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| CN102424635A (en) * | 2011-09-19 | 2012-04-25 | 河北省微生物研究所 | Preparation method for composite continuous cropping resistant agent specially used for strawberries |
| CN103329939A (en) * | 2013-07-10 | 2013-10-02 | 唐睿 | Compound fermentation formula and technology of compound amino acid purine mother liquor Niannianle |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102424635A (en) * | 2011-09-19 | 2012-04-25 | 河北省微生物研究所 | Preparation method for composite continuous cropping resistant agent specially used for strawberries |
| CN102424635B (en) * | 2011-09-19 | 2015-04-08 | 河北省微生物研究所 | Preparation method for composite continuous cropping resistant agent specially used for strawberries |
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