CN1393719A - Autoamtic calibration method - Google Patents
Autoamtic calibration method Download PDFInfo
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- CN1393719A CN1393719A CN 01113734 CN01113734A CN1393719A CN 1393719 A CN1393719 A CN 1393719A CN 01113734 CN01113734 CN 01113734 CN 01113734 A CN01113734 A CN 01113734A CN 1393719 A CN1393719 A CN 1393719A
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Abstract
An autoamtic calibration method for microscope includes such steps as under the preset multiple, calculating the proportional factor between the measured value and the actual value of a target image with standard sizes, and calculating the proportional factors for setting up other multiples according to the calculated factor. On actural measuring, multiplying the masured value by a corresponding proportional factor can obtain a more correct value, so reducing the error.
Description
The present invention relates to a kind of automatic calibration system, method, be particularly useful in the microscopical automated calibration system.
Owing in the transmission of light and gatherer process, unavoidably can produce error, image promptly in kind by Optics in Microscope partly and distortion in the process of collecting part (CCD), like this, the computer measured value is not the actual value of material picture in the microscope.
The invention provides a kind of automatic calibrating method that designs at above-mentioned error, purpose is to correct the distortion phenomenon that material picture produces when observed.
The present invention is achieved in that earlier under default multiple, calculate a certain the have measured value of standard-sized calibration target image and the scale-up factor between the actual value, calculate the scale-up factor of other default multiples again with this scale-up factor, when actual measurement, multiply by and observe the corresponding scale-up factor of multiple by measured value, can obtain one more near the measured value of actual value.
The calculating of concrete scale-up factor may further comprise the steps:
1,, use diameter to calibrate as the standard round of Φ at N times of object lens;
2, in normal window, open a width of cloth calibration circle image or take a calibration circle image by acquisition window is new;
3, according to image gray levels the standard round image is played up, and find and mark standard round image on all targets;
4, the height Y and the width X (pixel number) of basis of calculation circle;
5, compare with width Y with the height X that measures with the standard round actual diameter, obtain one group of horizontal and vertical scale-up factor respectively:
H
N=Φ/X V
N=Φ/Y
In a kind of microscopic system, the error scale-up factor of different multiples is relatively-stationary.Have the measured value of standard-sized calibration target image under a certain multiple by obtaining, thereby obtain the error scale-up factor under this multiple, when actual measurement, just can obtain one more near the picture size of actual value by calculating, reduced error rate, when especially the present invention realizes by computer program, quicker and more convenient.
Describe the present invention below in conjunction with accompanying drawing.
Fig. 1 is a basic flow sheet of the present invention.
In the specific embodiments of the invention, suppose to use diameter to calibrate, may further comprise the steps (as shown in Figure 1) as the standard round of Φ with N times of object lens:
1, in normal window, opens a width of cloth calibration circle image or take a calibration circle image by acquisition window is new;
2, identify the picture of standard round in picture by application software: according to image gray levels
The standard round image is played up, and find and mark standard round image on all targets;
3, give calibration circle image boundary rectangle;
4, calculate the height Y and the width X (pixel number) of boundary rectangle;
5, compare with width Y with the height X that measures with the standard round actual diameter, obtain one group of horizontal and vertical scale-up factor respectively:
51, H
N=physical size/boundary rectangle height=Φ/Y
52, V
N=physical size/boundary rectangle width=Φ/X
6, calculate the scale-up factor of other default multiples with the scale-up factor that obtains in the previous step;
Suppose to calculate M scale-up factor doubly
61、H
M=H
N×N/M
62、V
M=V
N×N/M
7, measure actual value: actual value=measured value * scale-up factor
Principle of work of the present invention: use earlier a certainly to have standard-sized calibration circle a certain multiple in the preset value is calibrated, calculate the scale-up factor between measured value and the actual value, calculate the scale-up factor of other default multiples again with this scale-up factor, when actual measurement, multiply by and observe the corresponding scale-up factor of multiple by measured value, can obtain one more near the measured value of actual value.
Use different microscopes all will calibrate again, the multiple that calibration is selected for use should satisfy the calibration target image near the form size, and is if exceed form, then inaccurate; If too small, then error is too big.If it is more accurate that measurement result requires, after measuring multiple, preferably each change calibrates again.
Claims (4)
1, a kind of automatic calibrating method may further comprise the steps:
Step 1: under default multiple, calculate a certain the have measured value of standard-sized calibration target image and the scale-up factor between the actual value;
Step 2, calculate the scale-up factor of other default multiples with above-mentioned scale-up factor;
Step 3, when actual measurement, multiply by and observe the corresponding scale-up factor of multiple by measured value, can obtain one more near the measured value of actual value.
2, a kind of automatic calibrating method according to claim 1 is characterized in that: described calibration target image is a calibration circle.
3, a kind of automatic calibrating method according to claim 2, with N times of object lens, when using diameter to calibrate as the standard round of Φ, calculate scale-up factor and may further comprise the steps:
Step 1, in normal window, open a width of cloth calibration circle image or take a calibration circle image by acquisition window is new;
Step 2, the standard round image is played up according to image gray levels, and find and mark standard round image on all targets;
The height Y and the width X (pixel number) of step 3, basis of calculation circle;
Step 4, compare with width Y with the height X that measures, obtain one group of horizontal and vertical scale-up factor: H respectively with the standard round actual diameter
N=Φ/X V
N=Φ/Y.
4, a kind of automatic calibrating method according to claim 3, wherein the concrete way of step 3 is: give calibration circle image boundary rectangle, calculate the height Y and the width X (pixel number) of boundary rectangle.
Priority Applications (1)
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CNB011137347A CN1156790C (en) | 2001-06-25 | 2001-06-25 | Autoamtic calibration method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CNB011137347A CN1156790C (en) | 2001-06-25 | 2001-06-25 | Autoamtic calibration method |
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Publication Number | Publication Date |
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CN1393719A true CN1393719A (en) | 2003-01-29 |
CN1156790C CN1156790C (en) | 2004-07-07 |
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CNB011137347A Expired - Lifetime CN1156790C (en) | 2001-06-25 | 2001-06-25 | Autoamtic calibration method |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103299321A (en) * | 2010-10-22 | 2013-09-11 | 奥达塞斯工业自动控制和信息学有限公司 | System and method for digitizing objects that can be designed in multiple steps with automatic calibration |
CN103673890A (en) * | 2013-11-08 | 2014-03-26 | 广东出入境检验检疫局检验检疫技术中心 | Length and cylindrical surface area measuring methods based on digital image analysis |
CN104457599A (en) * | 2014-12-31 | 2015-03-25 | 南京信息工程大学 | Online non-contact thin wire diameter measuring device and method |
CN105547342A (en) * | 2015-11-30 | 2016-05-04 | 武汉精测电子技术股份有限公司 | Industrial lens testing device and method on the basis of liquid crystal display panel |
CN108663792A (en) * | 2018-07-20 | 2018-10-16 | 芜湖市皖江光电仪器有限公司 | A method of it is eliminated automatically for digital comparison microscope difference of magnification |
-
2001
- 2001-06-25 CN CNB011137347A patent/CN1156790C/en not_active Expired - Lifetime
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103299321A (en) * | 2010-10-22 | 2013-09-11 | 奥达塞斯工业自动控制和信息学有限公司 | System and method for digitizing objects that can be designed in multiple steps with automatic calibration |
CN103673890A (en) * | 2013-11-08 | 2014-03-26 | 广东出入境检验检疫局检验检疫技术中心 | Length and cylindrical surface area measuring methods based on digital image analysis |
CN103673890B (en) * | 2013-11-08 | 2019-05-31 | 广东出入境检验检疫局检验检疫技术中心 | Length and cylinder area measurement method based on digital image analysis |
CN104457599A (en) * | 2014-12-31 | 2015-03-25 | 南京信息工程大学 | Online non-contact thin wire diameter measuring device and method |
CN105547342A (en) * | 2015-11-30 | 2016-05-04 | 武汉精测电子技术股份有限公司 | Industrial lens testing device and method on the basis of liquid crystal display panel |
CN105547342B (en) * | 2015-11-30 | 2017-11-17 | 武汉精测电子技术股份有限公司 | Industrial lens test device and method based on liquid crystal panel |
CN108663792A (en) * | 2018-07-20 | 2018-10-16 | 芜湖市皖江光电仪器有限公司 | A method of it is eliminated automatically for digital comparison microscope difference of magnification |
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CN1156790C (en) | 2004-07-07 |
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