CN1324980C - Method for preparing microoganism additives for ensiling lucerne - Google Patents
Method for preparing microoganism additives for ensiling lucerne Download PDFInfo
- Publication number
- CN1324980C CN1324980C CNB2005100286902A CN200510028690A CN1324980C CN 1324980 C CN1324980 C CN 1324980C CN B2005100286902 A CNB2005100286902 A CN B2005100286902A CN 200510028690 A CN200510028690 A CN 200510028690A CN 1324980 C CN1324980 C CN 1324980C
- Authority
- CN
- China
- Prior art keywords
- ensiling
- streptococcus lactis
- clover
- alfalfa
- ensilage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The present invention relates to a method for preparing an alfalfa ensilage microoganism additive, which belongs to the biotechnology field. The alfalfa ensilage microoganism additive is composed of 50 to 70 wt% of previously fermented juice and 30 to 50 wt% of MRS liquid culture medium in which streptococcus acidi lactici is preserved. The present invention comprises the following preparation steps: (1) selecting fresh alfalfa materials; (2) making previously fermented juice; (3) extracting and purifying streptococcus acidi lactici; (4) activating streptococcus acidi lactici; (5) mixing the previously fermented juice and the activated streptococcus acidi lactici in the MRS liquid culture medium according to proportion, and forming the alfalfa ensilage microoganism additive. The additive made by the present invention has better effect of reducing pH and improving ensilage quality than the effect achieved by singly using the two additives, namely the previously fermented juice and the streptococcus acidi lactici. After alfalfa processed with the additive is ensiled for 30 days, the pH value of the ensilage is reduced by 10.4 to 12.7% as compared with that of alfalfa processed by the previously fermented juice, the pH value of the alfalfa is obviously reduced in the ensilage preparing fermentation phase and the acidification prophase, and the ensilage quality is improved.
Description
Technical field
That the present invention relates to is a kind of preparation method of biological technical field, specifically is a kind of preparation method of microoganism additives for ensiling lucerne.
Background technology
Alfalfa (Medicago sativa L. is hereinafter to be referred as clover) is a kind of perennial leguminous forage, and nutritive value is abundant, is the forage grass of plant-eating animal high-quality, and the good reputation of " king of herbage " is arranged.For a long time, the preservation of alfalfa is mainly based on hay curing, and the disadvantage of this method is that the nutriment loss is serious.In recent years, Canada, Japan and other countries began to utilize the method for ensiling to preserve clover, had solved the serious problem of nutriment loss in the clover preservation process.Because the clover soluble sugar content is low, the buffer capacity height belongs to the leguminous forage of difficult ensiling, and directly the ensiling effect is bad, need use additive in the silage fermentation process.The commonplace additive types of external use at present has the mixture of formic acid, lactobacillus preparation, enzyme preparation and lactic acid bacteria and enzyme preparation etc.
Find through literature search prior art, (1997) such as Mitsuaki Ohshima propose the preparation method of fermented green juice (PreviouslyFermented Juice is abbreviated as PFJ) and the effect in alfalfa ensilage thereof first at Animal FeedScience Technology the 66th phase of magazine (129-137 page or leaf).The main manufacturing process of this method is as follows: fresh clover → shred → add adds in water → dipping → pulverizing (making beating) → filtration → filtrate that glucose → PFJ ferments → make.Because fermented green juice contains the multiple lactic acid bacteria culturers that derives from clover, the effect of obvious reduction ensilage pH value is arranged in alfalfa ensilage, and, fermented green juice is made simple, draw materials conveniently, have no side effect behind the edible ensilage of domestic animal, harmless in the ensiling manufacturing process, therefore, begin gradually to be applied aborning.As the lactic acid fermented startup factor, fermented green juice can promote the lactic acid bacteria population to enlarge in ensilage, suppresses the growth of other harmful bacterium, improves the alfalfa ensilage quality.Many studies have shown that its effect is more effective than the single lactic acid bacteria of inoculation, can significantly reduce the pH value of ensiling clover with fermented green juice ensiling clover.Yet, in the fermented green juice except that containing various lactobacillus, also contain multiple harmful bacterial classification, these bacterial classifications equally also have the effect that starts the factor, in the alfalfa ensilage process, induce harmful bacterial classification group to enlarge, thereby reduced or slowed down the effect that fermented green juice promotes the lactic acid bacteria expanding propagation better, make the ensiling clover can not sharply reduce the pH value of ensilage early stage in a short time in preparation yeast phase and acidifying, this be fermented green juice as the greatest drawback that additives for ensiling exists in alfalfa ensilage, have influence on ensiling clover high-quality fermentation and form high-quality ensilage.In further retrieval, yet there are no so far relevant from clover separating lactic acid streptococcus (Streptococcus lactis) do not see yet fermented green juice and streptococcus lactis mixed report as addictive for alfalfa silage as the report of additives for ensiling.
Summary of the invention
The present invention is directed to the deficiency and the defective of fermented green juice ensiling in the background technology, a kind of preparation method of microoganism additives for ensiling lucerne is provided, make its gained microoganism additives for ensiling lucerne, obvious reduction ensiling preparation yeast phase and acidifying clover pH in early stage value are arranged, improve the effect of ensiling quality.
The present invention is achieved by the following technical solutions, microoganism additives for ensiling lucerne of the present invention is made up of by the percentage by weight of 50-70%:30-50% fermented green juice (PFJ) and the MRS fluid nutrient medium of preserving streptococcus lactis, contains streptococcus lactis 2 * 10 at least in wherein every ml MRS fluid nutrient medium
7Cfu, method step is as follows:
(1) selects fresh clover material;
(2) making of fermented green juice;
(3) streptococcus lactis separation, purge process;
(4) streptococcus lactis activation;
(5) fermented green juice is mixed in proportion with the streptococcus lactis of activation, form microoganism additives for ensiling lucerne.
Described step (1) is specially: get fresh alfalfa plants, remove each about 10cm of base portion and top, remove major branch and thicker branch simultaneously, remaining sprout and leaf are kept, as the making material of fermented green juice;
Described step (2), be specially: fresh clover material is chopped into 3-5cm, add distilled water in clover (g) and 1: 2.5 ratio of water (ml), fully mix, with organizing pulverizer to smash to pieces at a high speed, left standstill 1 hour, double gauze filters, and adds the glucose or the suitable syrup of glucose amount, abundant stirring by the ratio of clover raw material: glucose=10g: 1g in filtrate, place 30 ℃ environment to ferment 48 hours, make fermented green juice;
Described step (3), be specially: with 60～100 times of fermented green juice dilutions, place the separating solids culture medium, 48h are cultivated down for 35～40 ℃ in medium pH=5.8～6.4, select that molten calcium circle is big, the bacterium colony of culture medium flavescence, be placed on MRS solid medium purifying, obtain streptococcus lactis (ST), the streptococcus lactis behind the purifying is preserved with the test tube that the MRS solid medium is housed, and the product acidity of this streptococcus lactis in 48 hours can see Table 1.
Described separating solids culture medium (100ml) prescription is: beef extract 1g, peptone 1g, yeast extract 1g, glucose 1g, tomato juice 20%, soil temperature 80 0.05%, calcium carbonate 2g, bromocresol green 0.01%, agar 1.5g, pH5.8～6.4,80ml distilled water.
Described MRS solid medium (100ml) prescription is: peptone 1g, beef extract 1g, yeast extract 0.5g, K
2HPO
40.2g, dibasic ammonium citrate 0.2g, sodium acetate 0.5g, glucose 2g, soil temperature 80 0.1%, MgSO
4.7H
2O 0.058g, MnSO
4.4H
2O 0.025g, agar 1.5g, pH=6.2,80ml distilled water, 121 ℃ of sterilization 15min.
The pH value of table 1 ST strain cultured solution and lactic acid content change (mmol/L)
| 0h | 6h | 12h | 24h | 48h | |
| The PH lactic acid content | 6.09±0.02 0.50±0.03 | 4.87±0.04 3.30±0.03 | 4.34±0.02 17.74±0.43 | 3.99±0.01 27.91±1.30 | 4.02±0.01 36.35±1.29 |
Described step (4) is specially: the streptococcus lactis that separates is placed in the MRS fluid nutrient medium activates, and 35～37 ℃ of activation temperatures, soak time 24～36 hours when activation is finished, contains streptococcus lactis 2 * 10 in every ml fluid nutrient medium at least
7Cfu.
Described MRS fluid nutrient medium (100ml) prescription is: peptone 1g, beef extract 1g, yeast extract 0.5g, K
2HPO
40.2g, dibasic ammonium citrate 0.2g, sodium acetate 0.5g, glucose 2g, soil temperature 80 0.1%, MgSO
4.7H
2O 0.058g, MnSO
4.4H
2O 0.025g, pH=6.2,80ml distilled water, 121 ℃ of sterilization 15min.
Described step (5), be specially: fermented green juice is mixed in the ratio of 50-70%: 30-50% with streptococcus lactis alive in the MRS fluid nutrient medium, form microoganism additives for ensiling lucerne, wherein every this additive of 10ml contains PFJ 5-7ml, streptococcus lactis 10
7～10
8Cfu, the per kilogram clover adds the 10-15ml additive during ensiling.
Characteristics of the present invention are: (1) streptococcus lactis is by separating in the clover; (2) two additives that have complementary functions of this streptococcus lactis and PFJ are mixed in proportion, form this additive, it is different from external streptococcus lactis (by obtaining in the non-clover material) or the PFJ method as addictive for alfalfa silage of using separately.Fermented green juice is the lactic acid fermented startup factor, and streptococcus lactis is the lactic acid bacteria culturers that the preparation yeast phase plays a major role, it is the fastest lactic acid bacteria culturers of preparation yeast phase breeding, two kinds of additives comprehensive and complementary, strengthened fermented green juice and promoted the effect that the ensiling clover is fermented, made this additive use the effect that better reduction pH and raising ensiling quality are arranged separately than fermented green juice and these two kinds of additives of streptococcus lactis.With the clover of this additive treating, through ensiling in 30 days, ensilage pH value reduced by 10.4～12.7% than handling with fermented green juice.Gained microoganism additives for ensiling lucerne of the present invention has obvious reduction ensiling preparation yeast phase and acidifying clover pH in early stage value, improves the effect of ensiling quality.
The specific embodiment
Provide following examples in conjunction with the inventive method step:
Embodiment one
1, selects fresh clover material: get fresh alfalfa plants, remove each about 10cm of base portion and top, remove major branch and thicker branch simultaneously, remaining sprout and leaf are kept, as the making material of fermented green juice;
2, fermented green juice is made: fresh clover material is chopped into 3-5cm, add distilled water in clover (g) and 1: 2.5 ratio of water (ml), fully mix, with organizing pulverizer to smash to pieces at a high speed, left standstill 1 hour, double gauze filters, the syrup that in filtrate, adds glucose or suitable glucose amount in the ratio of clover raw material: glucose=10g: 1g, fully stir, place 30 ℃ of fermentations 48 hours, make fermented green juice;
3, streptococcus lactis separation, purifying: with 100 times of fermented green juice dilutions, place the separating solids culture medium, medium pH=5.8, cultivate 48h down for 35 ℃, select that molten calcium circle is big, the bacterium colony of culture medium flavescence, be placed on MRS solid medium purifying, obtain streptococcus lactis (ST), the streptococcus lactis behind the purifying is preserved with the test tube that the MRS solid medium is housed.
Described separating solids culture medium (100ml) prescription is: beef extract 1g, peptone 1g, yeast extract 1g, glucose 1g, tomato juice 20%, soil temperature 80 0.05%, calcium carbonate 2g, bromocresol green 0.01%, agar 1.5g, pH=5.8,80ml distilled water.
Described MRS solid medium (100ml) prescription is: peptone 1g, beef extract 1g, yeast extract 0.5g, K
2HPO
4 -0.2g, dibasic ammonium citrate 0.2g, sodium acetate 0.5g, glucose 2g, soil temperature 80 0.1%, MgSO
4.7H
2O 0.058g, MnSO
4.4H
2O 0.025g, agar 1.5g, pH=6.2,80ml distilled water, 121 ℃ of sterilization 15min.
4, streptococcus lactis activation: the streptococcus lactis of separator well is placed in the MRS fluid nutrient medium activates, 35 ℃ of activation temperatures, soak time 24 hours when activation is finished, contains streptococcus lactis 2 * 10 in every ml fluid nutrient medium at least
7Cfu.
Described MRS fluid nutrient medium (100ml) prescription is: peptone 1g, beef extract 1g, yeast extract 0.5g, K
2HPO
40.2g, dibasic ammonium citrate 0.2g, sodium acetate 0.5g, glucose 2g, soil temperature 80 0.1%, MgSO
4.7H
2O 0.058g, MnSO
4.4H
2O 0.025g, pH=6.2,80ml distilled water, 121 ℃ of sterilization 15min.
5, the preparation of microoganism additives for ensiling lucerne: fermented green juice is mixed in 50%: 50% ratio with streptococcus lactis alive in the MRS fluid nutrient medium, form microoganism additives for ensiling lucerne, wherein every this additive of 10ml contains PFJ 5ml, streptococcus lactis 10
8Cfu, the per kilogram clover adds the 10ml additive during ensiling.
The alfalfa ensilage effect of table 2 embodiment one
| Handle | Sensible quality | pH | α＝0.05 | Protein | α＝0.05 |
| Additive-free this additive of PFJ | Inferior medium good | 5.10±0.12 4.87 ±0.16 4.32±0.18 | a b c | 17.25±0.51％ 20.31±0.12％ 22.07±0.19％ | c b a |
As seen from Table 2, with the clover of this additive treating, through ensiling in 30 days, the ensilage sensory evaluation reached good, and the sensory evaluation of PFJ is medium; Than PFJ processing low 12.7% and raising 8.7%, difference all reaches the level of signifiance respectively for pH value and protein content.
Embodiment two
1, selects fresh clover material: get fresh alfalfa plants, remove each about 10cm of base portion and top, remove major branch and thicker branch simultaneously, remaining sprout and leaf are kept, as the making material of fermented green juice;
2, fermented green juice is made: fresh clover material is chopped into 3-5cm, add distilled water in clover (g) and 1: 2.5 ratio of water (ml), fully mix, with organizing pulverizer to smash to pieces at a high speed, left standstill 1 hour, double gauze filters, the syrup that in filtrate, adds glucose or suitable glucose amount in the ratio of clover raw material: glucose=10g: 1g, fully stir, place 30 ℃ of fermentations 48 hours, make fermented green juice;
3, streptococcus lactis separation, purifying: with 80 times of fermented green juice dilutions, place the separating solids culture medium, medium pH=6.1, cultivate 48h down for 37 ℃, select that molten calcium circle is big, the bacterium colony of culture medium flavescence, be placed on MRS solid medium purifying, obtain streptococcus lactis (ST), the streptococcus lactis behind the purifying is preserved with the test tube that the MRS solid medium is housed;
Described separating solids culture medium (100ml) prescription is: beef extract 1g, peptone 1g, yeast extract 1g, glucose 1g, tomato juice 20%, soil temperature 80 0.05%, calcium carbonate 2g, bromocresol green 0.01%, agar 1.5g, pH=6.1,80ml distilled water.
Described MRS solid medium (100ml) prescription is identical with embodiment one.
4, streptococcus lactis activation: the streptococcus lactis that separates is placed in the MRS fluid nutrient medium activates, 37 ℃ of activation temperatures, soak time 30 hours when activation is finished, contains streptococcus lactis 2 * 10 in every ml fluid nutrient medium at least
7Cfu.
Described MRS fluid nutrient medium (100ml) prescription is identical with embodiment one.
5, the preparation of microoganism additives for ensiling lucerne: fermented green juice is mixed in 60%: 40% ratio with streptococcus lactis alive in the MRS fluid nutrient medium, form microoganism additives for ensiling lucerne, wherein every this additive of 10ml contains PFJ 6ml, streptococcus lactis 8 * 10
7Cfu, the per kilogram clover adds the 12ml additive during ensiling.
The alfalfa ensilage effect of table 3 embodiment two
| Handle | Sensible quality | pH | α＝0.05 | Protein | α＝0.05 |
| Additive-free this additive of PFJ | Inferior medium good | 5.10±0.12 4.87±0.16 4.38±0.21 | a b c | 17.25±0.51％ 20.31±0.12％ 22.17±0.24％ | c b a |
As seen from Table 3, with the clover of this additive treating, through ensiling in 30 days, the ensilage sensory evaluation reached good, and the sensory evaluation of PFJ is medium; Than PFJ processing low 11.9% and raising 9.2%, difference all reaches the level of signifiance respectively for pH value and protein content.
Embodiment three
1, selects fresh clover material: get fresh alfalfa plants, remove each about 10cm of base portion and top, remove major branch and thicker branch simultaneously, remaining sprout and leaf are kept, as the making material of fermented green juice;
2, fermented green juice is made: fresh clover material is chopped into 3-5cm, add distilled water in clover (g) and 1: 2.5 ratio of water (ml), fully mix, with organizing pulverizer to smash to pieces at a high speed, left standstill 1 hour, double gauze filters, the syrup that in filtrate, adds glucose or suitable glucose amount in the ratio of clover raw material: glucose=10g: 1g, fully stir, place 30 ℃ of fermentations 48 hours, make fermented green juice;
3, streptococcus lactis separation, purifying: with 60 times of fermented green juice dilutions, place solid medium, medium pH=6.4, cultivate 48h down for 40 ℃, select that molten calcium circle is big, the bacterium colony of culture medium flavescence, be placed on MRS solid medium purifying, obtain streptococcus lactis (ST), the streptococcus lactis behind the purifying is preserved with the test tube that the MRS solid medium is housed;
Described solid medium (100ml) prescription is: beef extract 1g, peptone 1g, yeast extract 1g, glucose 1g, tomato juice 20%, soil temperature 80 0.05%, calcium carbonate 2g, bromocresol green 0.01%, agar 1.5g, pH=6.4,80ml distilled water.
Described MRS solid medium (100ml) prescription is identical with embodiment one.
4, streptococcus lactis activation: the streptococcus lactis of separator well is placed in the MRS fluid nutrient medium activates, 37 ℃ of activation temperatures, soak time 36 hours when activation is finished, contains streptococcus lactis 2 * 10 in every ml fluid nutrient medium at least
7Cfu.
Described MRS fluid nutrient medium (100ml) prescription is identical with embodiment one.
5, the preparation of microoganism additives for ensiling lucerne: fermented green juice is mixed in 70%: 30% ratio with streptococcus lactis alive in the MRS fluid nutrient medium, form microoganism additives for ensiling lucerne, wherein every this additive of 10ml contains PFJ 7ml, streptococcus lactis 6 * 10
7Cfu, the per kilogram clover adds the 15ml additive during ensiling.
The alfalfa ensilage effect of table 4 embodiment three
| Handle | Sensible quality | pH | α＝0.05 | Protein | α＝0.05 |
| Additive-free this additive of PFJ | Inferior medium good | 5.10±0.12 4.87±0.02 4.41±0.18 | a b c | 17.25±0.51％ 20.31±0.12％ 22.01±0.19％ | c b a |
As seen from Table 4, with the clover of this additive treating, through ensiling in 30 days, the ensilage sensory evaluation reached good, and the sensory evaluation of PFJ is medium; Than PFJ processing low 10.4% and raising 8.4%, difference all reaches the level of signifiance respectively for pH value and protein content.
The preparation method of above-mentioned three embodiment and the technological process of production can be sketched and be:
Fresh clover → selected → chopping → dipping → pulverizing (making beating) → leave standstill → filter → the add glucose → PFJ → dilution of fermenting → make → streptococcus lactis cultivation → purifying → acquisition streptococcus lactis → activation → PFJ+ streptococcus lactis mixing → microoganism additives for ensiling lucerne.
Making difference between table 5 embodiment
| Embodiment | The PFJ extension rate | Medium pH | Cultivation temperature ℃ | Soak time h | Activation temperature ℃ |
| 1 2 3 | 100 80 60 | 5.8 6.1 6.4 | 35 38 40 | 24 30 36 | 35 37 37 |
Claims (3)
1, a kind of preparation method of microoganism additives for ensiling lucerne, it is characterized in that, described microoganism additives for ensiling lucerne is made up of by the percentage by weight of 50-70%: 30-50% fermented green juice and the MRS fluid nutrient medium of preserving streptococcus lactis, and preparation process is as follows:
(1) selects fresh clover material;
(2) making of fermented green juice is specially: the fresh alfalfa material that (a) will choose is chopped into 3-5cm; (b) in clover g and water ml1: 2.5 ratio adds distilled water, fully mixes, and with organizing pulverizer that mixed clover is smashed to pieces at a high speed, leaves standstill 1 hour, filters through double gauze; (c) in filtrate, add the glucose or the suitable syrup of glucose amount in the ratio of clover raw material: glucose=10g: 1g, fully stir, place 30 ℃ environment fermentation 48 hours, make fermented green juice;
(3) streptococcus lactis separation, purge process are specially: (a) with 60～100 times of fermented green juice dilutions, place the separating solids culture medium, medium pH=5.8～6.4 are cultivated 48h down for 35～40 ℃; (b) select that molten calcium circle is big, the bacterium colony of culture medium flavescence, be placed on MRS solid medium purifying, obtain streptococcus lactis;
Described separating solids culture medium prescription is: beef extract 1g, and peptone 1g, yeast extract 1g, glucose 1g, tomato juice 20%, soil temperature 80 0.05%, calcium carbonate 2g, bromocresol green 0.01%, agar 1.5g, pH 5.8～6.4;
Described MRS solid culture based formulas is: peptone 1g, beef extract 1g, yeast extract 0.5g, K
2HPO
40.2g, dibasic ammonium citrate 0.2g, sodium acetate 0.5g, glucose 2g, soil temperature 80 0.1%, MgSO
47H
2O0.058g, MnSO
44H
2O 0.025g, agar 1.5g, pH=6.2,121 ℃ of sterilization 15min;
(4) streptococcus lactis activation: be specially: the streptococcus lactis of separator well is placed in the MRS fluid nutrient medium activates, 35～37 ℃ of activation temperatures, soak time 24～36 hours when activation is finished, contains streptococcus lactis 2 * 10 in every ml MRS fluid nutrient medium at least
7Cfu;
Described MRS Liquid Culture based formulas is: peptone 1g, beef extract 1g, yeast extract 0.5g, K
2HPO
40.2g, dibasic ammonium citrate 0.2g, sodium acetate 0.5g, glucose 2g, soil temperature 80 0.1%, MgSO
47H
2O0.058g, MnSO
44H
2O 0.025g, pH=6.2,121 ℃ of sterilization 15min;
(5) fermented green juice is mixed in proportion with the streptococcus lactis of activation, form microoganism additives for ensiling lucerne.
2, the preparation method of microoganism additives for ensiling lucerne according to claim 1 is characterized in that, described step (1), be specially: get fresh alfalfa plants, remove each 10cm of base portion and top, remove major branch and thicker branch simultaneously, remaining sprout and leaf are kept.
3, the preparation method of microoganism additives for ensiling lucerne according to claim 1 is characterized in that, wherein every 10ml additive contains PFJ 5-7ml, streptococcus lactis 10
7～10
8Cfu, the per kilogram clover adds the 10-15ml additive during ensiling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100286902A CN1324980C (en) | 2005-08-11 | 2005-08-11 | Method for preparing microoganism additives for ensiling lucerne |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100286902A CN1324980C (en) | 2005-08-11 | 2005-08-11 | Method for preparing microoganism additives for ensiling lucerne |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1718076A CN1718076A (en) | 2006-01-11 |
| CN1324980C true CN1324980C (en) | 2007-07-11 |
Family
ID=35930119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100286902A Expired - Fee Related CN1324980C (en) | 2005-08-11 | 2005-08-11 | Method for preparing microoganism additives for ensiling lucerne |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1324980C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869230B (en) * | 2010-07-13 | 2013-01-16 | 山东省农业科学院畜牧兽医研究所 | Microbial additive for alfalfa haylage, preparation method and application thereof |
| CN102860412A (en) * | 2011-07-06 | 2013-01-09 | 吉林大学 | Co-production method for distilled spirit and livestock total nutrient feed through alfalfa fermentation |
| CN103053808B (en) * | 2013-01-25 | 2014-01-08 | 中国农业大学 | Silage additive and preparation method and application thereof |
| CN104193548B (en) * | 2014-08-11 | 2016-05-04 | 上海交通大学 | The bio-additive preparation method of a kind of alfalfa green manure synergy |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0329164A2 (en) * | 1988-02-18 | 1989-08-23 | The Calpis Food Industry Co., Ltd. | Lactic acid bacteria starter for preparation of silage and agent containing the bacteria in the viable state |
| RU1799395C (en) * | 1990-09-10 | 1993-02-28 | Институт Микробиологии И Вирусологии Ан@ Казсср | Strain of bacterium acidocaldarius used for straw ensilage |
| WO1999053775A1 (en) * | 1998-04-17 | 1999-10-28 | The Board Of Regents For Oklahoma State University | Propionibacterium p-63 for use in direct fed microbials for animal feeds |
| CN1530437A (en) * | 2003-03-14 | 2004-09-22 | 新疆威仕达生物工程股份有限公司 | Microbial ensiling strain and composite fungus, method for producing silage |
-
2005
- 2005-08-11 CN CNB2005100286902A patent/CN1324980C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0329164A2 (en) * | 1988-02-18 | 1989-08-23 | The Calpis Food Industry Co., Ltd. | Lactic acid bacteria starter for preparation of silage and agent containing the bacteria in the viable state |
| RU1799395C (en) * | 1990-09-10 | 1993-02-28 | Институт Микробиологии И Вирусологии Ан@ Казсср | Strain of bacterium acidocaldarius used for straw ensilage |
| WO1999053775A1 (en) * | 1998-04-17 | 1999-10-28 | The Board Of Regents For Oklahoma State University | Propionibacterium p-63 for use in direct fed microbials for animal feeds |
| CN1530437A (en) * | 2003-03-14 | 2004-09-22 | 新疆威仕达生物工程股份有限公司 | Microbial ensiling strain and composite fungus, method for producing silage |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1718076A (en) | 2006-01-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102864095B (en) | Lactobacillus plantarum used for silage alfalfa and use method thereof | |
| CN102907572A (en) | Banana silage microbial additive and preparation method thereof | |
| CN113273645B (en) | Silage method of mulberry green feed | |
| CN109770060A (en) | A method of preparing soybean stalk feed | |
| CN1324980C (en) | Method for preparing microoganism additives for ensiling lucerne | |
| CN1729829A (en) | Addictive for alfalfa silage | |
| CN1718077A (en) | Method for preparing additives for ensiling lucerne | |
| CN101965906A (en) | Preparation method of stylosanthes guianensis biological feed | |
| CN100381562C (en) | Microorganism additive of alfalfa ensilage | |
| CN115011515B (en) | Strain for increasing plant alcohol content in alfalfa silage and application thereof | |
| CN116849292A (en) | Fermented feed additive and preparation method and application thereof | |
| CN1320106C (en) | Preparation method of composite bacterial additive of alfalfa ensilage | |
| CN115669809A (en) | Corn silage additive | |
| CN1312274C (en) | Composite bacterial additive of alfalfa ensilage | |
| CN1304564C (en) | Preparation method of microorganism fermentation liquid for alfalfa ensilage | |
| CN112753867B (en) | Feed for improving functions of cattle liver and application thereof | |
| CN101731489A (en) | Special ensiling additive of Galega orientalis Lam.cv.Xinyin No.1 | |
| CN105707459A (en) | Production method of ramie and Phalaris tuberose cv.Weining mixed silage | |
| CN102450525A (en) | Leguminous forage silage complex bacteria additive | |
| CN111670998A (en) | Composite microbial inoculum suitable for silage corns in Ningxia region, preparation method and application | |
| CN115176886B (en) | Silage for high-protein low-fiber hybrid broussonetia papyrifera and preparation method thereof | |
| CN1304563C (en) | Microorganism fermentation liquid of alfalfa ensilage | |
| CN115786228B (en) | Lactobacillus genetically engineered bacterium and application thereof | |
| CN104651276B (en) | A kind of ferment agent for sour milk culture medium and its application | |
| CN116831224B (en) | Preparation method of fermented pennisetum sinese feed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070711 Termination date: 20100811 |