CN1321564C - Artificial progagation method for Anastatus spp. - Google Patents
Artificial progagation method for Anastatus spp. Download PDFInfo
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- CN1321564C CN1321564C CNB2005100027471A CN200510002747A CN1321564C CN 1321564 C CN1321564 C CN 1321564C CN B2005100027471 A CNB2005100027471 A CN B2005100027471A CN 200510002747 A CN200510002747 A CN 200510002747A CN 1321564 C CN1321564 C CN 1321564C
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Abstract
The present invention discloses an artificial procreation method for Anastatus spp, which comprises the steps that (1), the procreation of host ova, a, selecting and storing a tussah cocoon, b, heating the cocoon, c, storing a moth, d, laparotomizing the moth for collecting the oval and washing the oval, and e, disinfecting and airing the oval; (2), the collection and the procreation of hatching Anastatus spp, a, collecting the hatching Anastatus spp, b, purifying and rejuvenating the hatching Anastatus spp, c, increasing the hatching Anastatus spp, and d, refrigerating the hatching Anastatus spp; (3) sticking a card; (4), heating and feathering the Anastatus spp in the step c or d of (2); (5), packing and hanging the card; (6), ovipositing and parasitizing, (7), cultivating for growing; (8), detecting quality; (9), storing in low temperature; (10), heating for growing and preparing for transportation or release. The adoption of the technical scheme provided by the present invention realizes the Anastatus spp procreation with high grade, high efficiency and large batch and can manufacture on a large scale. The present invention is used for the technical field of biological control of agriculture and forestry pests, is used for preventing and controlling the agriculture and forestry pests such as litchi bugs, etc., and meets the requirements of procreation.
Description
Technical field
The present invention relates to the propagation production method of a kind of parasitic wasp, particularly a kind of anastatus japonicus artificial propagation production method belongs to the biological control technical fields of pest.
Background technology
The developing direction of international agricultural production is a sustainable agriculture, and the basis that increases productivity is to the protection of ecotope and construction, forms the economic and rural area application technology system efficiently of a cover.The biological control technology is particularly protected and the integrated control technique of breeding, discharge natural enemy will certainly become one of agent technology of sustainable agriculture development.In recent years, the research of domestic relevant natural enemy insect is used and is also made significant progress, biology and ecology to the numerous species natural enemy insect are studied, and explore its control potential to insect, self numerous approach of expansion and field application etc., all obtain more greatly, newer achievement.Natural enemy insects such as anastatus japonicus are discharged the field by breeding, are used to control agriculture and forestry injurious insects such as lichee stinkbug.But still in exploration, original propagation technique also rests on the manual small lot batch manufacture stage to the technology that the anastatus japonicus large scale artificial breeding produces.Exist host egg to collect and availability is low, in the breeding disease seriously infect, problem such as honeybee kind quality instability, not have the efficiently suitable technological process of production, and of poor quality, efficient is low, production scale is less, often can not meet the needs of production.
Summary of the invention
Technical problem to be solved by this invention is to avoid above-mentioned deficiency of the prior art, and proposes a kind of anastatus japonicus artificial progagation, and this technology has realized high-quality, bred anastatus japonicus efficiently, in enormous quantities, meets the needs of production.
Technical scheme provided by the present invention is: a kind of anastatus japonicus artificial propagation production method may further comprise the steps:
(1) produces host egg
A. the selection of tussah cocoon and storage: selecting does not have disease pupa, tender pupa, and female cocoon rate is more than 80%, and thousand kernel weight reaches the healthy tussah cocoon more than 11 kilograms; The tussah cocoon that chooses is housed in 0 ℃ ± 2 ℃, and in guarantor's cocoon storehouse of RH50-70%, cocoon is deposited with the layering of cocoon bed in the storehouse, or places cocoon cage, cocoon basket to preserve, and makes regular check on, and the silk cocoon temperature-sensitive does not evenly generate heat, does not make moist, do not go mouldy;
B. heating of cocoon: according to numerous honeybee plan warming cocoons date, before the warming cocoons cocoon is worn bunchiness, promptly pass the floating hair of cocoon afterbody with fine rule, worm base of a fruit ground outwardly, down, then it is worn into the cocoon string, it is indoor to hang warming cocoons, and the warming cocoons temperature heats up from low to high, and initial temperature is 10-14 ℃, increasing progressively 1 ℃ every day later on heats gradually, held stationary when rising to 18-20 ℃ the highlyest is no more than 25 ℃, RH.60-75%;
C. receive the moth storage: male moth sprouts wings Zao than female moth, for preventing the male and female mating, stimulates female moth from laying eggs, and receives male moth earlier, then the female moth of tussah that sprouts wings is deposited storage 24-48 hour under 0-2 ℃ of low temperature;
D. cut open the belly and adopt ovum and wash ovum: earlier female moth head is lifted, its belly being put into the machine of cuing open cuts open the belly and gets ovum again, ovum is through rinsing, after Ex-all is doped in impurity such as the polypide fragment of ovum intergranular and capsule, put into green ovum scavenger and remove immature ovum, remove with water rinse again and squeeze the broken broken chorion of immature ovum, green ovum rate is reduced to below 3%;
E. the sterilization of ovum with dry: cleaned ovum is sent into the ovum disinfection room, soaks taking-up after 10-15 minute, after the drier drying, enter the ovum workshop of drying in the air and dry in the shade with 0.1% bromogeramine liquid;
(2) gather and breed anastatus japonicus honeybee kind:
A. field bee kind;
B. honeybee kind purification and rejuvenation: the anastatus japonicus of gathering placed under 24-26 ℃, RH.60～70% condition grow, sprout wings honeybee after, be transferred in the vector ovum, impurity elimination is purified after breeding for 1～2 generation continuously, and, select good honeybee kind by parasitic rate, emergence bee hatching rate, fertility, property are detected test than, storage characteristic with to the search capability of target pest ovum;
C. the honeybee kind expands numerous: the expansion of carrying out anastatus japonicus honeybee kind is numerous, accumulates enough anastatus japonicus kind honeybee quantity;
D. honeybee kind refrigeration: when anastatus japonicus grew the phase in larva later stage, storage immediately went under 2～5 ℃, RH.50～60% condition to refrigerate;
(3) sticking card: select for use toughness strong, the good paper of glue and viscosity is good, the milky white adhesive easily done, utilize glue spreader the even scrubbing brush of milky white adhesive on paper, host egg, be bonded at securely on the paper then through sterilization, drying in the shade gets final product;
(4) anastatus japonicus among step (2) c or the d is heated under 24-26 ℃, RH.60～70% condition emergence;
(5) vanning hangs tag: the anastatus japonicus kind honeybee card that will newly sprout wings hangs over the inboard of bee-receiving box earlier, and then the host egg card is hung over the outside, and honeybee ovum ratio is 1: 8-12, and dress is complete obturages beehive;
(6) ovipositing hosts: at 25-27 ℃, RH.65-75%, and satisfy under the suitable illumination condition, allow anastatus japonicus ovipositing hosts on the host egg card, changed a host egg card in per two days, every generation honeybee kind connects honeybee 20-25 days continuously, and gives every day kind of honeybee fresh honey extra-nutrition;
(7) cultivate to grow: after postvaccinal host egg card is taken out, change 25-27 ℃, the growth chamber of RH.65-75% over to and be placed in the growth dish at the bottom of the gauze and continue to cultivate;
(8) quality inspection: every batch products parasitic rate, eclosion rate, property ratio are tested, and clearly identify;
(9) cryopreservation: lay eggs and grew the back 15-20 days, the egg parasitoid card is moved in 2-6 ℃ the low temperature storehouse accumulation and preserve, can preserve 50-80 days.
(10) growth of heating: according to putting the honeybee date, the honeybee card is continued to grow after put the desired accumulated temperature of honeybee transportation or discharge under 25-27 ℃, RH.65-75% condition.
The present invention has following advantage: determined the collection and the short term stored technology of host's egg of oak silkworm, improved mature egg rate 5-10%; Increase seed selection of honeybee kind and rejuvenation technique content, guaranteed kind honeybee quality, improved parasitic rate 5-15%; Basically accomplish sterile working, effectively controlled the disease in the numerous honeybee process of anastatus japonicus, realized high-quality, bred anastatus japonicus efficiently, in enormous quantities, be used to prevent and treat agriculture and forestry injurious insects such as lichee stinkbug.Adopt scheme provided by the invention to make the production of natural enemy insect anastatus japonicus not be subject to seasonal restrictions, make full use of factory building, equipment, manpower etc., the mechanization and the artificial climate control level of production process have been improved greatly, reduced cost, perfect advantage honeybee kind is selected and numerous bee technology, can carry out large-scale production, high-quality, breed anastatus japonicus efficiently, in enormous quantities, meet the needs of production.
Description of drawings
Fig. 1 is an anastatus japonicus artificial propagation production method flow chart of the present invention.
Embodiment
The specification requirement that discharges according to control area, numerous honeybee and field is as putting the honeybee amount, put the honeybee number of times, putting the artificial propagation production schedules of formulation anastatus japonicuss such as honeybee date.Specifically, mainly be to take place tentatively to determine to put the honeybee date with a situation arises period, put honeybee area and quantity according to target pest over the years, determine heat date and the quantity of kind of honeybee according to putting the reckoning of honeybee date then, coordinate the outbound of tussah simultaneously and heat date and quantity.Referring to accompanying drawing 1, the production method that anastatus japonicus is produced in artificial propagation is as follows:
1, produces host egg
A. the selection of tussah cocoon and storage: selecting does not have disease pupa, tender pupa, female cocoon rate more than 80%, thousand kernel weight reaches the healthy tussah cocoon more than 11 kilograms; The tussah cocoon that chooses is housed in 0 ℃ ± 2 ℃, and in guarantor's cocoon storehouse of RH60%, cocoon is deposited with the layering of cocoon bed in the storehouse, or places cocoon cage, cocoon basket to preserve, and makes regular check on, and guarantees that the silk cocoon temperature-sensitive does not evenly generate heat, does not make moist, do not go mouldy;
B. heating of cocoon: according to numerous honeybee plan warming cocoons date.Cocoon is worn bunchiness before the warming cocoons, promptly pass the floating hair of cocoon afterbody with fine rule, the cocoon string is worn into outwardly, down in worm base of a fruit ground, and it is indoor to hang warming cocoons.The warming cocoons temperature will be from low to high, and 12 ℃ of initial temperatures are heated gradually to increase progressively 1-2 ℃ every day, and held stationary when rising to 18-20 ℃ the highlyest is no more than 25 ℃, and relative moisture remains on about 70%.To establish the personal management in the warming cocoons process, be sure not to make temperature fluctuated.Observe record temperature, humidity round the clock, control tussah moth sprouts wings the date, and the warming cocoons chamber will keep air fresh, and floor cleaning is through bactericide disinfection commonly used.
C. receive moth and get ovum: silkworm moth generally sprouts wings during 2-5 in the afternoon, and male moth sprouts wings Zao than female moth, for preventing the male and female mating, stimulates female moth from laying eggs, and receives male moth earlier, the female moth of tussah that sprouts wings is deposited under 0-2 ℃ of low temperature preserved 30 hours then;
D. cut open the belly and wash ovum: be to guarantee the ovum cleanliness, earlier female moth head is lifted, again its belly is put into the machine of cuing open and cut open the belly and get ovum.Ovum is through rinsing, and Ex-all is put into green ovum scavenger and removed immature ovum after being doped in impurity such as the polypide fragment of ovum intergranular and capsule, removes crowded broken immature ovum and broken chorion with water rinse again, and green ovum rate is reduced to below 3%.
E. the sterilization of ovum with dry: cleaned ovum is sent into the ovum disinfection room, is that 0.1% bromogeramine liquid soaks taking-up after 10 minutes with concentration, enters the ovum workshop of drying in the air and dry in the shade after the drier drying.
2, gather and breed the honeybee kind:
A. field bee kind: gather anastatus japonicus honeybee kind from the field or introduce anastatus japonicus honeybee kind;
B. honeybee kind purification and rejuvenation: the anastatus japonicus of gathering placed under 25 ℃, RH.65% condition grow, sprout wings honeybee after, be transferred in the vector ovum, impurity elimination is purified after breeding for 2 generations continuously, and, select good honeybee kind by parasitic rate, emergence bee hatching rate, fertility, property are detected test than, storage characteristic with to the search capability of target pest ovum
C. the honeybee kind expands numerous: after selecting advantage anastatus japonicus honeybee kind, should with its refrigerate under the 2-4 ℃ of condition when the honeybee kind is bred standby.The general storage 30-40 days changes once numerous with egg of oak silkworm.Because the phase of depositing is long, eclosion rate is descended rapidly, become the vitality of honeybee also can die down.The honeybee kind can be placed in test tube or other containers, and guarantees the accurate and stable of reserve temperature.Choose from the collection of honeybee kind and to produce a large amount of production and use honeybee, numerous through 5 expansions, accumulate enough kind honeybee quantity.During the expansion of honeybee kind is numerous, adopt measures such as alternating temperature breeding, prevent the degeneration of honeybee kind.Take the 20 ℃ of alternating temperatures breedings in 12 hours 26 ℃ and 12 hours, the honeybee ratio that connects of strict control kind of honeybee guarantees means such as suitable single egg parasite number, cultivates strong honeybee.
D. honeybee kind refrigeration: when anastatus japonicus grew the phase in larva later stage, storage immediately went into to refrigerate under 2～5 ℃, RH.50～60% condition when the honeybee kind is bred standby;
3, sticking card: select for use toughness strong, the good paper of glue and viscosity is good, the milky white adhesive easily done, utilize glue spreader the even scrubbing brush of milky white adhesive on paper, host egg, be bonded at securely on the paper then through sterilization, drying in the shade gets final product;
4, with the emergence of heating under 25 ℃ of the anastatus japonicuss among step (2) c or the d, the RH.65% condition;
5, vanning hangs tag: the anastatus japonicus kind honeybee card that will newly sprout wings hangs over the inboard of bee-receiving box earlier, and then the host egg card is hung over the outside, and complete the obturaging of dress gets final product;
6, ovipositing hosts: at 26 ℃, RH.70%, and satisfy under the suitable illumination condition, allow anastatus japonicus ovipositing hosts on the host egg card, changed a host egg card in per two days, every generation honeybee kind can connect honeybee 23 days continuously, and gives every day kind of honeybee fresh honey extra-nutrition;
7, cultivate to grow: after postvaccinal host egg card was taken out, the growth chamber that changes 26 ℃ of temperature daytimes, 20 ℃ of evenings, RH.70% over to is placed in the growth dish at the bottom of the gauze continued to cultivate;
8, quality inspection: every batch products parasitic rate, eclosion rate, property ratio are tested, and clearly identify;
9, cryopreservation: lay eggs and grew back 20 days, the egg parasitoid card is moved in 2-4 ℃ the low temperature storehouse accumulation and preserve, deposit in 80 days, little to the eclosion rate influence.Be about to the bee pupa of emergence, refrigerate 21 days down at 5 ℃, its eclosion rate is about 95%.
10, the growth of heating: according to putting the honeybee date, the honeybee card is continued to grow to putting the desired accumulated temperature of honeybee, relative moisture remains on about 70%, prepares transportation or discharges.
Key link is equipped with special facility in production process, as adopts ovum machine, crowded green ovum machine, dewaterer, winnower winnowing machine, separator, glue spreader etc.The ring ring joins as connecing honeybee, lay eggs etc. in important production procedure.Sterile working is controlled and carried out to laying coop automatically with artificial climates such as growing the chamber, forms a large-scale industrialized breeding anastatus japonicus production line based on the artificial climate control of machinery basically, reached the leading level in the world.In large-scale industrialized numerous honeybee process, various pathogens are serious day by day to infecting of anastatus japonicus and host egg, cause parasitic rate, eclosion rate obviously to reduce, numerous honeybee decrease in efficiency, and production cost increases, even numerous honeybee is come a howler.Pressing in the production provides effective comprehensive preventive health measures.In the propagation production process of anastatus japonicus, with bromogeramine, concentration potassium permanganate liquid and uviol lamp respectively to the sterilization that carries out disinfection of host egg, room and apparatus.
Claims (1)
1, a kind of anastatus japonicus artificial propagation production method may further comprise the steps:
(1) produces host egg
A. the selection of tussah cocoon and storage: selecting does not have disease pupa, tender pupa, and female cocoon rate is more than 80%, and thousand kernel weight reaches the healthy tussah cocoon more than 11 kilograms; The tussah cocoon that chooses is housed in 0 ℃ ± 2 ℃, and in guarantor's cocoon storehouse of RH50-70%, cocoon is deposited with the layering of cocoon bed in the storehouse, or places cocoon cage, cocoon basket to preserve, and makes regular check on, and the silk cocoon temperature-sensitive does not evenly generate heat, does not make moist, do not go mouldy;
B. heating of cocoon: according to numerous honeybee plan warming cocoons date, before the warming cocoons cocoon is worn bunchiness, promptly pass the floating hair of cocoon afterbody with fine rule, worm base of a fruit ground outwardly, down, then it is worn into the cocoon string, it is indoor to hang warming cocoons, and the warming cocoons temperature heats up from low to high, and initial temperature is 10-14 ℃, increasing progressively 1 ℃ every day later on heats gradually, held stationary when rising to 18-20 ℃ the highlyest is no more than 25 ℃, RH.60-75%;
C. receive the moth storage: male moth sprouts wings Zao than female moth, for preventing the male and female mating, stimulates female moth from laying eggs, and receives male moth earlier, then the female moth of tussah that sprouts wings is deposited storage 24-48 hour under 0-2 ℃ of low temperature;
D. cut open the belly and adopt ovum and wash ovum: earlier female moth head is lifted, its belly being put into the machine of cuing open cuts open the belly and gets ovum again, ovum is through rinsing, after Ex-all is doped in the polypide fragment and capsule impurity of ovum intergranular, put into green ovum scavenger and remove immature ovum, remove with water rinse again and squeeze the broken chorion of broken immature ovum, green ovum rate is reduced to below 3%;
E. the sterilization of ovum with dry: cleaned ovum is sent into the ovum disinfection room, soaks taking-up after 10-15 minute, after the drier drying, enter the ovum workshop of drying in the air and dry in the shade with 0.1% bromogeramine liquid;
(2) gather and breed anastatus japonicus honeybee kind:
A. field bee kind;
B. honeybee kind purification and rejuvenation: the anastatus japonicus of gathering placed under 24-26 ℃, RH.60～70% condition grow, sprout wings honeybee after, be transferred in the vector ovum, impurity elimination is purified after breeding for 1～2 generation continuously, and, select good honeybee kind by parasitic rate, emergence bee hatching rate, fertility, property are detected test than, storage characteristic with to the search capability of target pest ovum;
C. the honeybee kind expands numerous: the expansion of carrying out anastatus japonicus honeybee kind is numerous, accumulates enough anastatus japonicus kind honeybee quantity;
D. honeybee kind refrigeration: anastatus japonicus grows larva during the later stage, and storage immediately goes under 2～5 ℃, RH.50～60% condition to refrigerate;
(3) sticking card: select for use toughness strong, the good paper of glue and viscosity is good, the milky white adhesive easily done, utilize glue spreader the even scrubbing brush of milky white adhesive on paper, host egg, be bonded at securely on the paper then through sterilization, drying in the shade gets final product;
(4) anastatus japonicus among step (2) c or the d is heated under 24-26 ℃, RH.60～70% condition emergence;
(5) vanning hangs tag: the anastatus japonicus kind honeybee card that will newly sprout wings hangs over the inboard of bee-receiving box earlier, and then the host egg card is hung over the outside, and honeybee ovum ratio is 1: 8-12, and dress is complete obturages beehive;
(6) ovipositing hosts: at 25-27 ℃, RH.65-75%, and satisfy under the suitable illumination condition, allow anastatus japonicus ovipositing hosts on the host egg card, changed a host egg card in per two days, every generation honeybee kind connects honeybee 20-25 days continuously, and gives every day kind of honeybee fresh honey extra-nutrition;
(7) cultivate to grow: after postvaccinal host egg card is taken out, change 25-27 ℃, the growth chamber of RH.65-75% over to and be placed in the growth dish at the bottom of the gauze and continue to cultivate;
(8) quality inspection: every batch products parasitic rate, eclosion rate, property ratio are tested, and clearly identify;
(9) cryopreservation: lay eggs and grew the back 15-20 days, the egg parasitoid card is moved in 2-6 ℃ the low temperature storehouse accumulation and preserve, can preserve 50-80 days;
(10) growth of heating: according to putting the honeybee date, the honeybee card is continued to grow after put the desired accumulated temperature of honeybee transportation or discharge under 25-27 ℃, RH.65-75% condition.
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| CN101356906B (en) * | 2008-09-16 | 2010-12-15 | 广东省昆虫研究所 | Artificial propagation method and apparatus of egg parasitoids |
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| CN104839049B (en) * | 2015-06-08 | 2018-04-06 | 广东省农业科学院植物保护研究所 | A kind of Anastatus japonicus propagation device of honeybee ovum separation and numerous honeybee method |
| CN107372361B (en) * | 2017-07-14 | 2020-12-11 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | Ultralow temperature freezing method for bee eggs and matched tool thereof |
| CN108849750B (en) * | 2017-09-12 | 2021-04-23 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | Artificial breeding method of alfalfa leaf cutting bees |
| CN108513954A (en) * | 2018-04-11 | 2018-09-11 | 福建省农业科学院植物保护研究所 | A kind of method of quick inoculation trichogramma |
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