CN1304101C - Size-uniform agarose gel microball and its preparing method - Google Patents
Size-uniform agarose gel microball and its preparing method Download PDFInfo
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- CN1304101C CN1304101C CNB2004100000879A CN200410000087A CN1304101C CN 1304101 C CN1304101 C CN 1304101C CN B2004100000879 A CNB2004100000879 A CN B2004100000879A CN 200410000087 A CN200410000087 A CN 200410000087A CN 1304101 C CN1304101 C CN 1304101C
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- agarose
- agarose gel
- preparation
- water
- size homogeneous
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- 239000011543 agarose gel Substances 0.000 title claims description 30
- 239000011806 microball Substances 0.000 title description 29
- 238000000034 method Methods 0.000 title description 16
- 239000000839 emulsion Substances 0.000 claims abstract description 63
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 43
- 239000004005 microsphere Substances 0.000 claims abstract description 33
- 238000001816 cooling Methods 0.000 claims abstract description 14
- 238000004945 emulsification Methods 0.000 claims abstract description 11
- 238000013019 agitation Methods 0.000 claims abstract description 8
- 229920000936 Agarose Polymers 0.000 claims description 40
- 238000002360 preparation method Methods 0.000 claims description 39
- 239000003995 emulsifying agent Substances 0.000 claims description 11
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- 238000010438 heat treatment Methods 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 3
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- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Colloid Chemistry (AREA)
Abstract
Description
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CNB2004100000879A CN1304101C (en) | 2004-01-12 | 2004-01-12 | Size-uniform agarose gel microball and its preparing method |
Applications Claiming Priority (1)
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CNB2004100000879A CN1304101C (en) | 2004-01-12 | 2004-01-12 | Size-uniform agarose gel microball and its preparing method |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1640539A CN1640539A (en) | 2005-07-20 |
CN1304101C true CN1304101C (en) | 2007-03-14 |
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CNB2004100000879A Expired - Lifetime CN1304101C (en) | 2004-01-12 | 2004-01-12 | Size-uniform agarose gel microball and its preparing method |
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CN (1) | CN1304101C (en) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SG131015A1 (en) * | 2005-09-15 | 2007-04-26 | Millipore Corp | Method and apparatus for making porous agarose beads |
CN101293191B (en) * | 2007-04-25 | 2011-11-09 | 中国科学院过程工程研究所 | Agarose gelatin microsphere preparation method |
EA019920B1 (en) | 2007-12-03 | 2014-07-30 | ДСМ АйПи АССЕТС Б.В. | System and method for producing beads |
CN101486799B (en) * | 2008-01-16 | 2012-05-23 | 中国科学院过程工程研究所 | Agarose gel microsphere and preparation thereof |
CN101314648B (en) * | 2008-03-18 | 2010-12-22 | 天津大学 | Macromolecule blot gelose polymer microsphere and preparation method thereof |
CN103831066B (en) * | 2012-11-27 | 2015-12-02 | 山东鼎欣生物科技有限公司 | Microsphere preparation technology is analysed by CM agar basic unit |
CN103923355B (en) * | 2014-04-09 | 2016-03-09 | 无锡百运纳米科技有限公司 | A kind of preparation method of agarose-hydroxyapatite composite microspheres |
CN104826605B (en) * | 2015-04-24 | 2017-09-29 | 广州极泰生物科技有限公司 | One kind prepares highly cross-linked uniform particle size Ago-Gel medium using ultrasonic inverse suspension method |
CN105170042B (en) * | 2015-09-17 | 2017-10-20 | 湖州师范学院 | A kind of method of the antibody separation based on magnetic microsphere |
CN105504334B (en) * | 2015-12-30 | 2019-07-23 | 浙江工业大学 | A kind of agarose crystalline substance gel matrix particle and preparation method thereof with super large hole |
CN105777942B (en) * | 2016-03-19 | 2017-12-22 | 绿麒(厦门)海洋生物科技有限公司 | The agarose gel microsphere production system and its production technology of uniform particle diameter |
CN112266427B (en) * | 2020-11-06 | 2022-04-19 | 内蒙古科技大学 | Hydrophobic agarose aerogel microspheres and preparation method thereof |
CN114191848B (en) * | 2021-12-06 | 2022-12-30 | 武汉瑞法医疗器械有限公司 | Method for cleaning agarose microspheres |
CN114702732B (en) * | 2022-06-07 | 2022-08-26 | 江苏集萃智能液晶科技有限公司 | Polymer particle with double-size pore channel and preparation method thereof |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4839111A (en) * | 1987-02-02 | 1989-06-13 | The University Of Tennessee Research Corporation | Preparation of solid core liposomes |
CN1316450A (en) * | 2001-03-24 | 2001-10-10 | 天津大学 | Process for preparing magnetic sepharose microspheres by both-phase (oil and water) method |
-
2004
- 2004-01-12 CN CNB2004100000879A patent/CN1304101C/en not_active Expired - Lifetime
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4839111A (en) * | 1987-02-02 | 1989-06-13 | The University Of Tennessee Research Corporation | Preparation of solid core liposomes |
CN1316450A (en) * | 2001-03-24 | 2001-10-10 | 天津大学 | Process for preparing magnetic sepharose microspheres by both-phase (oil and water) method |
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Publication number | Publication date |
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CN1640539A (en) | 2005-07-20 |
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Date | Code | Title | Description |
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C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Zhejiang Zhengguang Industrial Co.,Ltd. Assignor: Institute of Process Engineering, Chinese Academy of Sciences Contract fulfillment period: 2008.9.26 to 2018.9.26 Contract record no.: 2008330002027 Denomination of invention: Size-uniform agarose gel microball and its preparing method Granted publication date: 20070314 License type: Exclusive license Record date: 20081117 |
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LIC | Patent licence contract for exploitation submitted for record |
Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2008.9.26 TO 2018.9.26; CHANGE OF CONTRACT Name of requester: ZHEJIANG PROVINCE ZHENGGUAN INDUSTRY CO., LTD. Effective date: 20081117 |
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CX01 | Expiry of patent term | ||
CX01 | Expiry of patent term |
Granted publication date: 20070314 |