CN1283440A - Artificial skin and its preparing process and application - Google Patents
Artificial skin and its preparing process and application Download PDFInfo
- Publication number
- CN1283440A CN1283440A CN 99112354 CN99112354A CN1283440A CN 1283440 A CN1283440 A CN 1283440A CN 99112354 CN99112354 CN 99112354 CN 99112354 A CN99112354 A CN 99112354A CN 1283440 A CN1283440 A CN 1283440A
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- artificial skin
- collagen
- film
- gauze
- preparation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Materials For Medical Uses (AREA)
Abstract
An artificial collagen-type skin with 70-90% of water content is prepared from pig skin through immersing in ash, neutralization, extracting collagen, filter, concentrating and shaping. Its advantages include simple preparing process and convenient application.
Description
The invention belongs to medical artificial skin with and methods for making and using same.
For many years people's artificial skin majority of attempting to be used for the skin injury patient prepares from collagen derivants such as animal skins.The ZL89102123.X patent of invention is repeatedly to press with beaters' skin processing bak stay, makes through operations such as pore-formings again; The ZL87106118.X patent of invention is to make skin membrane with Carapax et Plastrum Testudinis, fur, chitosan and Chinese herbal medicine etc. as raw material.The product of these class methods must be got rid of the danger of infectious disease, and service life is shorter, and other then remain defective at aspects such as antibiotic, the nutrition of wound surface, maintenance body fluid by biomaterial or synthetic material and the two compounded artificial skin.
The objective of the invention is to overcome above-mentioned defective, provide a kind of and can form protecting film after being used on the wound surface, increase skin-nourishing, promote wound surface to absorb, make wound healing rapidly and use collagen protein type artificial skin easily.
The objective of the invention is to be achieved through the following technical solutions:
A kind of artificial skin, the composition of skin membrane mainly contains collagen protein and water, it is characterized in that water is 70-90% (weight).
The preparation method of this artificial skin is as follows:
With extracting collagen by standard method after the Corii Sus domestica defat, it is characterized in that: carry out polish thereafter:
The first step; The collagen liming is soaked collagen 30-60 days in 15-30 ℃, 1-5% lime liquor, through washing, is neutralized to PH 4.5-6, again washing;
Second step: collagen extraction, under low temperature 35-50 ℃, carry glue with the distilled water of collagen and 20 times of collagen weight, tell extract after, to carry glue at 55-70 ℃ of following secondary with the volume distilled water, slowly heat up simultaneously, finally extracting below 95 ℃, the time is 1-3 hour.
The 3rd step: the sheet film of filter, concentrate, the 0.2-2 millimeters thick being made in molding.
This artificial skin is packed after sterilization can use with metal foil paper.
The film that adopts this preparation method to make is a wet film, can be coated on one deck gauze, with gauze as support.Also can stick with paste at the two-sided collagen that all applies of gauze lining.
The application process of this artificial skin is characterised in that the artificial skin film with band gauze lining directly sticks on the wound surface, can take the gauze lining off after half an hour.Look wound site and area and both can directly wrap up outside artificial skin, it is deposited also can to dry in the air, and directly the replacing of wrapping wrapping is also very convenient.
Adopt product of the present invention, need not to cooperate other therapies, can be applicable to any position of human body, nontoxic, non-stimulated, not have allergy, no rejection, permeability good.The composition of this product contains several seed amino acids of high-load needed by human, is digested, absorbs by human body especially easily simultaneously, to promoting wound healing and skin nutrition comparatively outstanding effect is arranged.Product permeability of the present invention is strong; has good adhesive force; the very fast gauze lining that just sees through is attached on the wound surface after sticking wound surface; absorb the injury transudate; and form one deck dissolving film, this film not only absorbs the moisture of wound surface but also can preserve moisture, and can make wound surface keep moistening moist; protect the wound tissue that appears, reduced the consumption of infection and albumen body fluid.Because these characteristics have just shortened wound surface and have generated the time of new granulation and the time of cicatrization, and cicatrix is smooth, has both reduced patient's human body misery, reduces psychic pain again.Product of the present invention also has because of transparent is convenient to observe advantage such as wound surface cleaning, and in addition, this product also can be used as the health and beauty facial film of skin.Made 16 cases with product of the present invention clinically, respond well, except that the burn and scald of II ° depth injure the avulsion of skin damaged, III ° burn and scald is cured also very rapid.If any a case, breast, back II degree burn surface area 30%, wherein the III degree accounts for 10%, only with the i.e. recovery from illness in 15 days of this artificial skin.
Below introduce embodiment:
Example 1, a kind of artificial skin is characterized in containing the moisture content of 70-90% except that the several amino acids of needed by human.Preparation method; With fresh porcine skin (the heavy 120kg of pig), except that fleshing, fat spin tissue, epidermal tissue and subcutaneous tissue etc., be cut into small pieces, by the collagen extracting method extraction collagen of standard.Carry out polish then:
1, liming: in 25 ℃ (± 2 ℃), 3% lime liquor, soaked 50 days, washing, it is 5.5 that the reuse dilute hydrochloric acid is neutralized to PH, washes with distilled water at last.
2, extraction: to the collagen distilled water collagen is carried glue with 20 times under 45 ℃ ± 2 ℃, tell extract after, with the distilled water of same volume 65 ℃ ± make to carry for the second time glue below 2 ℃, slowly intensification is finally being carried below 95 ℃ simultaneously, the time is 75 minutes.
3, to collagen solution filter, concentrate, molding, make the sheet film of 1 (± 0.2) millimeters thick, pack back high temperature sterilize sterilization with masking foil, high temperature moment (140 ℃ 5 seconds) sterilization treatment, sheet film moisture 75%.
Table 1 is various amino acid whose content (concentration expressed in percentage by weight) in the collagen protein after the polish, no matter the proteic concentration height of collagen quantity, its composition is constant substantially.Table 2 is concrete data of the preparation technology of other several embodiment.Among these embodiment after the 1st, 2,5, the 7 routine film forming result of use better.Table 1
Table 2
| Title | Percentage composition g/100g | Title | Percentage composition g/100g |
| Aspartic Acid | ????5.49 | Glycine | ????23.47 |
| Threonine | ????1.89 | Alanine | ????8.86 |
| Serine | ????3.17 | Cystine | ????0.94 |
| Glutamic acid | ????9.54 | Valine | ????0.30 |
| Proline | ????14.16 | Methionine | ????0.47 |
| Isoleucine | ????1.19 | Lysine | ????3.92 |
| Leucine | ????2.46 | Histidine | ????0.60 |
| Tyrosine | ????0.48 | Arginine | ????9.00 |
| Phenylalanine | ????1.94 | Hydroxyproline | ????4.79 |
Claims (5)
1, a kind of artificial skin contains the several amino acids of needed by human such as glutamic acid, proline, glycine, it is characterized in that containing in the artificial skin film water of 70-90%.
2, the preparation method of artificial skin as claimed in claim 1, extract collagen by standard method after adopting the Corii Sus domestica defat, it is characterized in that, carry out polish then, the collagen liming of (1) step is soaked collagen 30-60 days in 15-30 ℃ 1-5% lime liquor, through washing, being neutralized to PH is 4.5-6, again washing; (2) step collagen extraction is carried glue with collagen at 35-50 ℃ with collagen and 20 times of distilled water to collagen of weight, tell extract after, distilled water with same volume makes to carry for the second time glue at 55-70 ℃, slowly heat up simultaneously, finally extracting below 95 ℃, extraction time is 1-3 hour; (3) goes on foot filtration, concentrated, molding, makes the artificial skin sheet film of 0.5-5 millimeters thick.
3, the preparation method of artificial skin as claimed in claim 2 is characterized in that the collagen paste of making evenly is coated on as on the gauze that sets off, can list or double-coated.
4, the preparation method of artificial skin as claimed in claim 2 is characterized in that artificial skin sheet film is by metal foil paper packing back sterilization.
5, the application process of artificial skin as claimed in claim 1 is characterized in that directly sticking on patient's wound surface with the artificial skin film of band gauze lining, takes off after half an hour and can wrap up outside the artificial skin film or not wrap up and the dew treatment of drying in the air after gauze serves as a contrast.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99112354 CN1283440A (en) | 1999-08-04 | 1999-08-04 | Artificial skin and its preparing process and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99112354 CN1283440A (en) | 1999-08-04 | 1999-08-04 | Artificial skin and its preparing process and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1283440A true CN1283440A (en) | 2001-02-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 99112354 Pending CN1283440A (en) | 1999-08-04 | 1999-08-04 | Artificial skin and its preparing process and application |
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| CN (1) | CN1283440A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105997300A (en) * | 2016-05-12 | 2016-10-12 | 江西高大生物医疗科技有限公司 | Automatic production line of artificial skin |
| CN111855742A (en) * | 2019-12-16 | 2020-10-30 | 西安交通大学 | Electronic skin structure for detecting damage degree of explosion to human body and detection method |
| CN113101009A (en) * | 2021-04-19 | 2021-07-13 | 河南亚都实业有限公司 | Medical artificial skin applicator device |
-
1999
- 1999-08-04 CN CN 99112354 patent/CN1283440A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105997300A (en) * | 2016-05-12 | 2016-10-12 | 江西高大生物医疗科技有限公司 | Automatic production line of artificial skin |
| CN105997300B (en) * | 2016-05-12 | 2017-08-25 | 江西高大生物医疗科技有限公司 | A kind of artificial skin automatically generates line |
| CN111855742A (en) * | 2019-12-16 | 2020-10-30 | 西安交通大学 | Electronic skin structure for detecting damage degree of explosion to human body and detection method |
| CN111855742B (en) * | 2019-12-16 | 2021-11-19 | 西安交通大学 | Electronic skin structure for detecting damage degree of explosion to human body and detection method |
| CN113101009A (en) * | 2021-04-19 | 2021-07-13 | 河南亚都实业有限公司 | Medical artificial skin applicator device |
| CN113101009B (en) * | 2021-04-19 | 2023-05-30 | 河南亚都实业有限公司 | Medical artificial skin applicator device |
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| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |