A kind of production of gourmet powder
Technical field
The present invention relates to a kind of glutamate production method.Specifically, it relates to 1) in the sweat, the carbonate (and/or oxide and/or hydroxide) that adds divalence (or trivalent) metal directly generates the glutamate solid of respective metal; 2) the glutamate solid after filtration, washing removes all kinds of impurity such as contained inorganic salts, protein, pigment, sugar in the zymotic fluid; 3) metathesis reaction is taken place in glutamate and sodium carbonate (and/or NaOH), generate carbonate (and/or oxide and/or hydroxide) precipitation and the monosodium glutamate solution of respective metal; This metathesis reaction also can be carried out in the presence of activator, makes reaction more complete, and yield improves; 4) carbonate (and/or oxide and/or hydroxide) precipitation is returned the sweat use; 5) monosodium glutamate solution is through decolouring, and condensing crystallizing obtains the technology of pure sodium glutamate product.
Background technology
Monosodium glutamate, its chemical name are sodium glutamate.Crucial function is arranged, extensively be used in aspects such as food, medicine, industry and agricultural.Monosodium glutamate is mainly made by microbial fermentation starch method, but there is following problem in existing technology:
1. the sweat product is a monoammonium glutamate, rather than glutamic acid or glutamic acid are received (monosodium glutamate).
In the glutamic acid fermentation process, along with the continuous generation of glutamic acid, the pH value of zymotic fluid constantly reduces, and the glutamic acid bacterium is produced suppress, and for the optimum condition that keeps fermenting, adopts the method for stream liquid feeding ammonia alkaline agent, with the neutrality of maintenance system pH6.8~7.0; So the consequence that produces is, what obtain from fermentation tank is monoammonium glutamate, rather than desirable glutamic acid or glutamic acid are received (monosodium glutamate), extraction process is in the removal of impurities of purifying after making follow-up monosodium glutamate, also needs additionally to finish monoammonium glutamate → glutamic acid → glutamic acid and receives the conversion procedure of (monosodium glutamate).
2. monoammonium glutamate transforms the monosodium glutamate process, increases a large amount of production costs, produces serious environmental pollution.
The conversion procedure of monoammonium glutamate → glutamic acid in now producing all adopts to add the concentrated sulfuric acid, and to regulate the isoelectric point (pH2.8~3.2) of zymotic fluid (pH6.8~7.0) to glutamic acid, freezing precipitation is separated out the glutamic acid method;
Monoammonium glutamate+sulfuric acid → glutamic acid (precipitation)+ammonium sulfate (solution)
From the liquefied ammonia that adds of stream and the sulfuric acid effect of only regulating pH as can be seen of above-mentioned flow process, finally discharge with the form of ammonium sulphate waste liquor, produce 1 ton of monosodium glutamate and consume the concentrated sulfuric acid~800Kg, ammoniacal liquor~600Kg, produce 1 ton of ammonium sulfate and discharge 20 tons of high concentrated organic wastewaters, become one of key component in the flood tide gustatory production wastewater.
Summary of the invention
The objective of the invention is to seek a kind of production of gourmet powder, in process of production, need not to add liquefied ammonia and sulfuric acid and regulate pH, thereby shortened production technology, reduced production cost largely, the three wastes environmental pollution major part in the glutamate production is eliminated.
Generate the glutamate of divalence or trivalent metal according to design provided by the present invention: a, direct fermentation: as 100%, wherein add two enzyme sugar 5%~30% with the zymotic fluid gross weight, add K
2HPO
4Amount 0.5%~3% adds MgSO
4Amount 0.03%~0.1%, add corn steep liquor 0.2%~2.0%, add phenol red 0.06%~0.20%, the urea addition is 1.4~2.2%, all the other are deionized water and/or fermented waste fluid, after the sterilization in 120 ℃/10 minutes, add 5.4% glutamate producing bacterium strain kind amount, 33 ℃~37 ℃ of control fermentation temperatures, control air quantity 96r/min~110r/min, continue to flow during the fermentation and add the corresponding divalence that its glutamate can precipitate or the carbonate of trivalent metal under neutrallty condition, oxide, hydroxide at least a, addition directly generates the glutamate solid precipitation of corresponding divalence or trivalent metal can keep zymotic fluid pH 6.0~7.5 in the zymotic fluid;
B, glutamate metathesis reaction: after above-mentioned zymotic fluid leaves standstill, the most of mycelium that is suspended in the zymotic fluid is discharged from zymotic fluid; After the glutamate solid precipitation of zymotic fluid bottom is separated by filtration again, adding concentration is 10%~70% sodium carbonate and/or sodium hydroxide solution, the at least a of carbonate that metathesis reaction generates monosodium glutamate solution and respective metal, oxide, precipitation of hydroxide taken place, and course of reaction is as follows:
Glutamate+sodium carbonate and/or NaOH →
At least a precipitation of the carbonate of monosodium glutamate solution+divalence or trivalent metal, oxide, hydroxide.
In the technology of the present invention, the technology that zymotic fluid directly generates sodium glutamate is almost completely consistent with existing production of gourmet powder, only is that the urea addition is 30%~50% of a former technology, and preferable urea addition is 38~42% of a former technology; Carbonate (and/or oxide and/or hydroxide) addition of divalence (or trivalent) metal to be can keeping zymotic fluid pH 6.0~7.5, and preferable addition is for keeping zymotic fluid pH in 6.8~7.0 amount; Directly generate the glutamate of corresponding divalence (or trivalent) metal in the zymotic fluid, rather than original monoammonium glutamate.
The concrete fermentation condition that directly generates the glutamate of divalence or trivalent metal: as 100%, wherein add two enzyme sugar 5%~30% with the zymotic fluid gross weight, preferable pair of enzyme sugar addition 16%~20%; Add K
2HPO
4Amount 0.5%~3%, preferable K
2HPO
4Amount 0.16%~2%; Add MgSO
4Amount 0.03%~0.1%, preferable MgSO
4Amount 0.05%~0.07%; Add corn steep liquor amount 0.2%~2.0%, preferable corn steep liquor amount 0.8%~1.2%; Add phenol red amount 0.06%~0.20%, preferable phenol red amount 0.12%~0.14%; The urea addition is 1.4~2.2%, and preferable urea addition is 1.7~1.9%; All the other are deionized water (and/or the described fermented waste fluid of claim 9).After the sterilization in 120 ℃/10 minutes, add 5.4% glutamate producing bacterium strain kind amount, 33 ℃~37 ℃ of control fermentation temperatures, control air quantity 96r/min~110r/min.The carbonate (and/or oxide and/or hydroxide) that continues to flow during the fermentation and add divalence (or trivalent) metal, addition to be can keeping zymotic fluid pH 6.0~7.5, and preferable addition is for keeping zymotic fluid pH in 6.8~7.0 amount; Directly generate the glutamate solid precipitation of corresponding divalence (or trivalent) metal in the zymotic fluid.
The divalence of above indication (or trivalent) metal is meant the metal that its carbonate (and/or oxide and/or hydroxide) precipitates, as Al under neutrallty condition
3+, Ba
2+, Bi
3+, Ca
2+, Co
2+, Co
3+, Cu
2+, Fe
2+, Fe
3+, Mg
2+, Ni
3+, pb
2+, Sb
3+, Sn
2+, Zn
2+Deng.
Zymotic fluid becomes glutamate solid and fermentation waste saccharide liquid two parts after filtration, the fermentation waste saccharide liquid is not owing to wherein add any chemical reagent, can be direct/or behind partial concentration, filtering and impurity removing, zymotechnique is returned in circulation, replace deionized water to use, alleviate the environmental pollution burden greatly.
The glutamate solid that filtration obtains is after hot wash, adding concentration is 10%~70% sodium carbonate (and/or NaOH) solution, preferable sodium carbonate (and/or NaOH) solution concentration is 35%~40%, and the carbonate (and/or oxide and/or hydroxide) that generates monosodium glutamate solution and respective metal with sodium carbonate generation metathesis reaction precipitates:
Glutamate+sodium carbonate (and/or NaOH) →
Monosodium glutamate solution+carbonate (and/or oxide and/or hydroxide) precipitation
In the metathesis reaction of glutamate and sodium carbonate (and/or NaOH) generation, can add glutamic acid quaternary ammonium salt activator, under its effect, can make metathesis reaction more complete, make yield improve 1%~15%.Wherein activator level is 5/100000ths to 5/10000ths of a glutamate amount, optimum amount be ten thousand of glutamate amount/.
Carbonate (and/or oxide and/or hydroxide) precipitation is returned sweat, is used in the generation glutamate.
Above-mentioned sodium glutamate is 35~45 ℃ in temperature adds down that 0.1% active carbon decolours, removal of impurities, the time is 0.25~0.5 hour, and the monosodium glutamate solution that obtains clarifying obtains pure sodium glutamate product (monosodium glutamate) through condensing crystallizing.
The outstanding advantage that the present invention has is as follows: 1. eliminated electricity such as freezing and from handing over skill, therefore do not needed to add any sulfuric acid, liquefied ammonia in new technology; 2. divalence (or trivalent) metal has been realized unrestrictedly recycling in production process dexterously, does not therefore produce any pollution; 3. do not use other any chemicals that has nothing to do with the product composition in the production process, produce without any polluting; 4. the fermentation waste saccharide liquid can return circulating fermentation, alleviates the environmental pollution burden greatly; 5. production process labour intensity is low, and production site is little; 6. compare with existing production technology, technological process is short, produces the yield height, has reduced production cost significantly.
Description of drawings
Fig. 1 is sodium glutamate production technology figure.
The specific embodiment
Next step comes further to introduce technology of the present invention in conjunction with the embodiments.
Embodiment one: fermentation method generates calcium glutamate
1. bacterial classification: N6 produces bacterial strain (Fujian Province Jianyang Wuyi MSG Co., Ltd provides)
2. one-level culture medium:
2.1 glucose 2.5%, urea 0.58%, K
2HPO
40.1%, MgSO
40.06%, corn steep liquor 3%.NaOH transfers pH6.7,50ml packing/500ml triangular flask.Sterilization in 120 ℃/20 minutes.
2.2 condition of culture: 33 ℃ ± 0.2 ℃, eight layers gauze of temperature, reciprocating type shaking table (amplitude 8.5cm frequency 98r/min).
2.3 plant age: 9 hours.
3. fermented and cultured:
3.1 fermentation medium: two enzyme sugar 18%; K
2HPO
40.18%; MgSO
40.06%; Corn steep liquor 1.0%; Phenol red 0.12%; (is 100% with the zymotic fluid gross weight); Sterilization in 120 ℃/10 minutes.The urea addition is 1/10 (an existing production technology 40%) of two enzymes sugar amounts; Continue to flow during the fermentation and add CaCO
3, addition is for keeping the interior pH of fermentation tank 6.8~7.0.
3.2 kind amount: 5.4%
3.3 temperature control: 33 ℃~37 ℃.
3.4 air quantity control: 96r/min~110r/min.
The fermentation result: the calcium glutamate precipitation capacity is 12.4%, and monoammonium glutamate content is 0%.
Embodiment two: the double decomposition operation
Zymotic fluid in the example one becomes calcium glutamate solid and fermentation waste saccharide liquid two parts after filtration, and the fermentation waste saccharide liquid is through concentrating 30%, and zymotechnique is returned in circulation behind the filtering and impurity removing, replaces water to use, to the glutamic acid no any adverse effect of normally fermenting.
The calcium glutamate solid 5Kg that filtration obtains is through hot wash, to eluate colourless after, place and decompose bucket, adding 4.8Kg concentration is 35% sodium carbonate liquor, with sodium carbonate metathesis reaction takes place under the effect of 0.5g glutamic acid quaternary ammonium salt activator and generates 18.4Kg monosodium glutamate solution and 1.8Kg precipitation of calcium carbonate.Concentration of sodium glutamate is 30.4% in the monosodium glutamate solution, and purity is 99.8%.
Sweat is returned in precipitation of calcium carbonate, and stream adds and is used in the generation calcium glutamate.
Above-mentioned sodium glutamate through activated carbon decolorizing, concentrate, crystallization obtains the qualified msg product of 5.4Kg.
Embodiment three: fermentation method generates zinc glutamate
1. bacterial classification: N6 produces bacterial strain (Fujian Province Jianyang Wuyi MSG Co., Ltd provides)
2. one-level culture medium:
2.1 glucose 2.5%, urea 0.58%, K
2HPO
40.1%, MgSO
40.06%, corn steep liquor 3%.NaOH transfers pH6.7,50ml packing/500ml triangular flask.Sterilization in 120 ℃/20 minutes.
2.2 condition of culture: 33 ℃ ± 0.2 ℃, eight layers gauze of temperature, reciprocating type shaking table (amplitude 8.5cm frequency 98r/min).
2.3 plant age: 9 hours.
3. fermentation condition:
3.1 two enzyme sugar 18%; K
2HPO
40.18%; MgSO
40.06%; Corn steep liquor 1.0%; Phenol red 0.12% (is 100% with the zymotic fluid gross weight); Sterilization in 120 ℃/10 minutes.The urea addition is 1/10 (an existing production technology 40%) of two enzymes sugar amounts; Continuing to flow during the fermentation adds ZnO, and addition is for keeping the interior pH of fermentation tank 6.8~7.0.
3.2 kind amount: 5.4%
3.3 temperature control: 33 ℃~37 ℃.
3.4 air quantity control: 96r/min~110r/min.
The fermentation result: the zinc glutamate precipitation capacity is 11.7%, and monoammonium glutamate content is 0%.
Embodiment four: the double decomposition operation
Zymotic fluid in the example three becomes zinc glutamate solid and fermentation waste saccharide liquid two parts after filtration, and the fermentation waste saccharide liquid is through concentrating 30%, and zymotechnique is returned in circulation behind the filtering and impurity removing, replaces water to use, to the glutamic acid no any adverse effect of normally fermenting.
The zinc glutamate solid 5.2Kg that filtration obtains is through hot wash, to eluate colourless after, place and decompose bucket, add 3.7Kg concentration and be 35% sodium hydroxide solution, metathesis reaction takes place generate 16.2Kg monosodium glutamate solution and 1.67Kg zinc oxide precipitation.Concentration of sodium glutamate is 28.8% in the monosodium glutamate solution, and purity is 99.8%.
Zinc oxide precipitation is returned sweat, and stream adds and is used in the generation zinc glutamate.
Above-mentioned sodium glutamate through activated carbon decolorizing, concentrate, crystallization obtains the qualified msg product of 5.2Kg.