CN1278598C - Method for producing fermented food of tremella mesenterica yeast spore and food additive - Google Patents
Method for producing fermented food of tremella mesenterica yeast spore and food additive Download PDFInfo
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- CN1278598C CN1278598C CNB2004100223773A CN200410022377A CN1278598C CN 1278598 C CN1278598 C CN 1278598C CN B2004100223773 A CNB2004100223773 A CN B2004100223773A CN 200410022377 A CN200410022377 A CN 200410022377A CN 1278598 C CN1278598 C CN 1278598C
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Abstract
The present invention relates to a method for producing food and food additives through the fermentation of golden jelly fungus yeast-shaped spores. The present invention is characterized in that the method carries out according to the following procedures: the golden jelly fungus food or the food additives is processed by culturing strains of the golden jelly fungus yeast-shaped spores, liquor seeds of golden jelly fungus yeast-shaped bacterial strains, the golden jelly fungus yeast-shaped spores in a table concentrator and golden jelly fungi in a fermentation tank and collecting fermentation liquor growing in the table concentrator or the fermentation tank so as to be processed in a mode of homogenate. The present invention overcomes the defects existing in the prior art, and solves the problems of low production of the golden jelly fungi which are produced in a mode of fermentation by using the hybrid bacterial strains and deficient raw materials. The fermentation technology of the present invention greatly improves producing time in the process of producing the golden jelly fungus food and the food additives, and simultaneously, reduces the dosage for processing the golden jelly fungi and the raw materials of the food additives; the present invention has the advantages of polysaccharide, great quality of the golden jelly fungi, unique frankincense scent for the processed food and the food additives.
Description
Technical field
The present invention relates to food technology field, the method for especially golden ear yeast shape spore fermenting and producing food and food additives.
Background technology
Gold ear (Tremella aurantialba Bandoni et Zang) is the food and medicament dual-purpose fungi of Basidiomycetes Tremellales Tremellaceae Tremella, mainly is distributed in China southwest, and golden ear artificial cultivation successfully (comprises Duan Mu, cultivating in bag).
Progressively put on market with believe what one is told product and food additives of golden fungus polysaccharides, the gold of golden ears or side handles of a utensil entity and hybrid bacterial strain [golden ear (Tremella aurantialba Bandoni et Zang) and hair Boreostereum vibrans (Stereum hirsutum (willd:Fr.) S.F.Gray)] fermenting and producing.Because culture technique and forest protection aspect do not have enough golden ears or side handles of a utensil entity or fruit body converted products that market is provided.And when believing what one is told product or food additives with hybrid bacterial strain fermenting and producing gold, because a little less than the growth of tremella silk, hair Boreostereum vibrans is preponderated, hybrid bacterial strain is sent out product that alcohol produces based on hair Boreostereum vibrans, and golden ear is few.
Summary of the invention
The objective of the invention is to solve the problem that exists in the golden ear production, a kind of condition at the growth of golden ear yeast shape spore is provided, improves golden ear output and produce the believe what one is told method of product or food additives of the gold that comprises polysaccharide by the submerged fermentation technology.
The tremella strain of yeast shape (yeast-like) spore that the present invention obtains by spore separation, yaasm-17 serves as to produce bacterial strain with toruloid tremella strain, produces gold believe what one is told product and food additives by the submerged fermentation technology.Can produce in the bioactive golden ear born of the same parents of tool and exocellular polysaccharide by fermentation.Can be made into nutritious food or food additives after the product homogenized of submerged fermentation.Under the concrete grammar:
Carry out according to the following steps, by weight percentage raw materials used:
1) golden ear yeast shape spore culture of strains: with golden ear yeast shape spore inoculating on improved PDA medium slant 20～28 ℃ cultivated 7～10 days, improving the PDA medium and be 10% potato boils liquid and adds 0.05～0.2% dusty yeast or cream, 0.05～0.2% peptone, 0.05～0.2% dipotassium hydrogen phosphate, 0.05～0.2% magnesium sulfate, 2% glucose or sucrose;
2) cultivation of golden ear yeast shape strain liquid seed: in 5～10% analysis for soybean powder, 5～10% corn flour, 0.05～0.2% dipotassium hydrogen phosphate, 0.05 add water surplus in～0.2% magnesium sulfate, stir and be divided in sterilization back cooling in the triangular flask, insert the test tube kind, 20～28 ℃ of 100～300rpm shaking tables were cultivated 3～10 days.
3) shaking table of golden ear yeast shape spore is cultivated: in 5～15% analysis for soybean powder, 5～15% corn flour, 0.05～0.2% dipotassium hydrogen phosphate, 0.05 add water surplus in～0.2% magnesium sulfate, stir and be divided in sterilization back cooling in the triangular flask, insert to cultivate 5～10% previous step gained seed of base unit weight, 20～28 ℃, 100～300rpm shaking table were cultivated 5～8 days.
4) fermentation tank culture of golden ear: in 5～15% analysis for soybean powder, 5～15% corn flour, 0.05～0.2% dipotassium hydrogen phosphate, 0.05 add water surplus in～0.2% magnesium sulfate, stir and be contained in sterilization back cooling in the fermentation tank, insert to cultivate 5～10% previous step gained seed of base unit weight, ventilation 0.5～2L/min, 100～300rpm stir 20～28 ℃ and cultivated 5～8 days.
5) zymotic fluid of shaking table or fermentation tank growth is collected after, carry out homogenized, be processed into gold believe what one is told product or food additives.
The method of above-described golden ear yeast shape spore fermenting and producing food and food additives is:
1, the preparation of golden ear beverage is: with the zymotic fluid of shaking table or fermentation tank growth collect carry out homogenized after, directly be processed into golden ear beverage or make the auxiliary material of beverage.
2, the believe what one is told preparation of product additive of gold is: carry out homogenized after the zymotic fluid of shaking table or fermentation tank growth is collected, after the homogenate drying the gold of the off-white powder product additive of believing what one is told.
3, the extraction of golden fungus polysaccharides is: will play the zymotic fluid behind the jar to leave standstill 12～24 hours, boil processing, filter, boiling filtrate concentrates with vacuum, when being concentrated into 20% left and right sides of original volume, concentrate adds the alcohol of 2 times of volumes 95%, leaves standstill after stirring 12 hours, collecting precipitation, after the drying the golden fungus polysaccharides of off-white powder.
The present invention has overcome the defective that prior art exists, and the golden ear that has solved with the hybrid bacterial strain fermenting and producing yields poorly, the problem of insufficient raw material.Believe what one is told product and food additives when having improved product greatly with fermentation technique production gold of the present invention, reduced the consumption of processing golden believe what one is told product and food additives raw material simultaneously, the golden fungus polysaccharides of producing by the present invention has good quality, and the food of processing and food additives have unique frankincense flavor.
Embodiment (used percentage all is weight percentage)
Embodiment 1: cultivate
1) the test tube kind of golden ear yeast shape bacterial strain is cultivated: golden ear yeast shape spore inoculating on improved PDA medium slant 25 ℃ cultivated 7 days, improve the PDA medium and be 10% potato and boil liquid and add 0.05% dusty yeast, 0.05% peptone, 0.05% dipotassium hydrogen phosphate (K
2HPO
4), 0.05% magnesium sulfate (MgSO
4), 2% glucose.
2) liquid seeds of golden ear yeast shape bacterial strain is cultivated: add water surplus in 5% analysis for soybean powder, 5% corn flour, 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, stir and be divided in sterilization back cooling in the triangular flask, insert the test tube kind, 20 ℃ of 100rpm shaking tables were cultivated 10 days.
3) shaking table of golden ear yeast shape bacterial strain is cultivated: add water surplus in 10% analysis for soybean powder, 10% corn flour, 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, stir and be divided in sterilization back cooling in the triangular flask, insert to cultivate 5% previous step (cultivations) gained seed of base unit weight, 20 ℃ of 100rpm shaking tables cultivations 8 days.
4) fermentation tank culture of golden ear yeast shape bacterial strain: in 10% analysis for soybean powder, 10% corn flour, 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, add water surplus, stir and be contained in sterilization back cooling in the fermentation tank, insert to cultivate 5% previous step (cultivation) gained seed of base unit weight, ventilation 0.5L/min, 100rpm stir 20 ℃ and cultivated 8 days.
Embodiment 2: cultivate
1) the test tube kind of golden ear yeast shape bacterial strain is cultivated: golden ear yeast shape spore inoculating on improved PDA medium slant 25 ℃ cultivated 7 days, improve the PDA medium and be 10% potato and boil liquid and add 0.2% yeast extract, 0.2% peptone, 0.1% dipotassium hydrogen phosphate, 0.1% magnesium sulfate, 2% sucrose.
2) liquid seeds of golden ear yeast shape bacterial strain is cultivated: add water surplus in 10% analysis for soybean powder, 10% corn flour, 0.2% dipotassium hydrogen phosphate, 0.2% magnesium sulfate, stir and be divided in sterilization back cooling in the triangular flask, insert the test tube kind, 28 ℃ of 300rpm shaking tables were cultivated 3 days.
3) shaking table of golden ear yeast shape bacterial strain is cultivated: add water surplus in 15% analysis for soybean powder, 15% corn flour, 0.2% dipotassium hydrogen phosphate, 0.2% magnesium sulfate, stir and be divided in sterilization back cooling in the triangular flask, insert to cultivate 10% previous step (cultivations) gained seed of base unit weight, 28 ℃ of 300rpm shaking tables cultivations 3 days.
4) fermentation tank culture of golden ear yeast shape bacterial strain: in 15% analysis for soybean powder, 15% corn flour, 0.2% dipotassium hydrogen phosphate, 0.2% magnesium sulfate, add water surplus, stir and be contained in sterilization back cooling in the fermentation tank, insert to cultivate 10% previous step (cultivation) gained seed of base unit weight, ventilation 2L/min, 300rpm stir 28 ℃ and cultivated 5 days.
Embodiment 3: golden ear beverage
After the zymotic fluid collection with shaking table or fermentation tank growth among the embodiment 1,2, carry out homogenized, homogenate is collected clear liquid with method centrifugal or that filter, clear liquid contains multiple polysaccharide, protein and mineral element, can directly be processed into golden ear beverage or make the auxiliary material of beverage, the beverage of processing is a milky, and certain sugariness is arranged under the situation of not sugaring, tool frankincense flavor.
Embodiment 4: golden ear additive
Carry out homogenized after the zymotic fluid collection with shaking table or fermentation tank growth among the embodiment 1,2, homogenate contains multiple polysaccharide, protein and mineral element, after the homogenate drying off-white powder (golden ear additive), dry powder joins wheaten food such as processing bread, cake, biscuit in the flour with 5～10% ratio, the wheaten food of producing is nutritious, tool frankincense flavor, mouthfeel is good.
Embodiment 5: extract golden fungus polysaccharides
Zymotic fluid after among the embodiment 1,2 following jar was left standstill 12-24 hour, boil processing, boiling liquid plate basket filters or centrifugal way is collected filtrate, and filtrate is more sticking, sugar content is higher, filtrate is filtered with vacuum, and when being concentrated into 20% left and right sides of original volume, concentrate adds the alcohol of 2 times of volumes 95%, left standstill after stirring 12 hours, with filtering or centrifugal way collecting precipitation, dried off-white powder is golden fungus polysaccharides (crude polysaccharides), and crude polysaccharides is based on mucopolysaccharides.
Claims (4)
1, a kind of golden ear yeast shape spore fermenting and producing food and food additives method is characterized in that carrying out according to the following steps, and be by weight percentage raw materials used:
1) golden ear yeast shape spore culture of strains: with golden ear yeast shape spore inoculating on improved PDA medium slant 20～28 ℃ cultivated 7～10 days, improving the PDA medium and be 10% potato boils liquid and adds 0.05～0.2% dusty yeast or cream, 0.05～0.2% peptone, 0.05～0.2% dipotassium hydrogen phosphate, 0.05～0.2% magnesium sulfate, 2% glucose or sucrose;
2) cultivation of golden ear yeast shape strain liquid seed: in 5～10% analysis for soybean powder, 5～10% corn flour, 0.05～0.2% dipotassium hydrogen phosphate, 0.05 add water surplus in～0.2% magnesium sulfate, stir and be divided in sterilization back cooling in the triangular flask, insert step 1) gained test tube kind, 20～28 ℃ of 100～300rpm shaking tables were cultivated 3～10 days.
3) shaking table of golden ear yeast shape spore is cultivated: in 5～15% analysis for soybean powder, 5～15% corn flour, 0.05～0.2% dipotassium hydrogen phosphate, 0.05 add water surplus in～0.2% magnesium sulfate, stir and be divided in sterilization back cooling in the triangular flask, insert to cultivate 5～10% step 2 of base unit weight) the gained seed, 20～2 ℃, 100～300rpm shaking table were cultivated 5～8 days.
4) fermentation tank culture of golden ear: in 5～15% analysis for soybean powder, 5～15% corn flour, 0.05～0.2% dipotassium hydrogen phosphate, 0.05 add water surplus in～0.2% magnesium sulfate, stir and be contained in sterilization back cooling in the fermentation tank, insert to cultivate 5～10% step 3) gained seed of base unit weight, ventilation 0.5～2L/min, 100～300rpm stir 20～28 ℃ and cultivated 5～8 days.
5) zymotic fluid of shaking table or fermentation tank growth is collected after, carry out homogenized, be processed into gold believe what one is told product or food additives.
2, golden ear yeast shape spore fermenting and producing food according to claim 1 and food additives method, the preparation that it is characterized in that golden ear beverage is: with the zymotic fluid of shaking table or fermentation tank growth collect carry out homogenized after, directly be processed into golden ear beverage or make the auxiliary material of beverage.
3, golden ear yeast shape spore fermenting and producing food according to claim 1 and food additives method, it is characterized in that the believe what one is told preparation of product additive of gold is: carry out homogenized after the zymotic fluid of shaking table or fermentation tank growth is collected, after the homogenate drying the gold of the off-white powder product additive of believing what one is told.
4, golden ear yeast shape spore fermenting and producing food according to claim 1 and food additives method, the extraction that it is characterized in that golden fungus polysaccharides is: will play the zymotic fluid behind the jar to leave standstill 12～24 hours, boil processing, filter, boil filtrate and concentrate, be concentrated into 20% o'clock of original volume with vacuum, concentrate adds the alcohol of 2 times of volumes 95%, left standstill after stirring 12 hours, collecting precipitation, after the drying the golden fungus polysaccharides of off-white powder.
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|---|---|---|---|
| CNB2004100223773A CN1278598C (en) | 2004-04-21 | 2004-04-21 | Method for producing fermented food of tremella mesenterica yeast spore and food additive |
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|---|---|---|---|
| CNB2004100223773A CN1278598C (en) | 2004-04-21 | 2004-04-21 | Method for producing fermented food of tremella mesenterica yeast spore and food additive |
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| CN1561667A CN1561667A (en) | 2005-01-12 |
| CN1278598C true CN1278598C (en) | 2006-10-11 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101348833B (en) * | 2008-07-16 | 2010-12-01 | 江苏同源堂生物工程有限公司 | ITS sequence of Tremella aurantiablba Bandoni and Stereum hirsutum and method for identifying golden fungus using the same |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250722B (en) * | 2011-07-04 | 2013-01-09 | 江苏同源堂生物工程有限公司 | Method for preparing gingko and tremella mesenterica fruit wine through fermentation by taking gingko as substrate |
| CN102908303B (en) * | 2012-11-19 | 2014-10-08 | 江南大学 | Method for preparing tremella aurantialba fermented whitening solution |
| CN103416222B (en) * | 2013-07-31 | 2015-06-24 | 常州大学 | Lepista nuda spore cultivation and ripening method through hypha shake flasks and liquid submerged fermentation process |
| CN103555787A (en) * | 2013-10-16 | 2014-02-05 | 江南大学 | Preparation method of tremella aurantialba fermentation liquid polysaccharide with anti-oxidation activity |
| CN116391737B (en) * | 2023-02-21 | 2024-05-28 | 江南大学 | A method for improving dough or batter with fungal fermentation product |
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2004
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101348833B (en) * | 2008-07-16 | 2010-12-01 | 江苏同源堂生物工程有限公司 | ITS sequence of Tremella aurantiablba Bandoni and Stereum hirsutum and method for identifying golden fungus using the same |
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| CN1561667A (en) | 2005-01-12 |
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