CN1278116C - Probe of fiber optic chemical sensor - Google Patents

Probe of fiber optic chemical sensor Download PDF

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Publication number
CN1278116C
CN1278116C CN 200410001755 CN200410001755A CN1278116C CN 1278116 C CN1278116 C CN 1278116C CN 200410001755 CN200410001755 CN 200410001755 CN 200410001755 A CN200410001755 A CN 200410001755A CN 1278116 C CN1278116 C CN 1278116C
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optical fiber
chemical sensor
fibre optic
reflective mirror
optic chemical
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CN1558214A (en
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熊海江
李新霞
陈坚
刘欢
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XINJIANG FUKESI BIOTECHNOLOGY DEVELOPMENT Co Ltd
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XINJIANG FUKESI BIOTECHNOLOGY DEVELOPMENT Co Ltd
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Abstract

The present invention relates to a probe of an optical fiber chemical sensor, which comprises a jacket layer, an optical fiber and a sensitive film, wherein the detection end of the optical fiber is provided with the sensitive film; alternatively, the probe comprises a sleeve tube, an optical fiber and a reflective mirror, wherein the reflective mirror is arranged at the outer end of the sleeve tube, at least two sample inlet is arranged in the middle of the sleeve tube; alternatively, the probe comprises a sleeve tube, an optical fiber and a reflective mirror, wherein an adjustable optical length rod is arranged at the outer end of the sleeve tube in a screw thread mode or an inserting mode, and the reflective mirror is arranged at the inner end of the adjustable optical length rod. The probe can be used for monitoring the characteristic measurement of liquid or gas substances in real time in an on-position mode, is especially used for the measurement of the dissolution degree or the release degree of a solid drug preparation, and can be used for the measurement of blood compositions; the probe can be made into a micro type, a special type and a general type, and can use light sources of ultraviolet / visible / near-infrared absorption, reflection and phosphorescent / fluorescent / Raman spectrum radiation, etc.

Description

The fibre optic chemical sensor probe
One, technical field:
The present invention relates to utilize fibre optic chemical sensor to carry out the probe of liquid or gaseous matter characteristic measurement, it is a kind of fibre optic chemical sensor probe, be applied to mensuration especially, also can be applicable to mensuration composition in the blood to solid pharmaceutical preparation (tablet, capsule etc.) dissolution rate or release.
Two, background technology:
" solid pharmaceutical preparation (tablet, capsule etc.) dissolution rate or drug release determination " is that whole world medicine is identified essential project.Drug dissolution is determined at quality and the new aspects such as dosage form research of development that improves pharmaceutical preparation, and is essential.The application of dissolution rate method at first is conventional drug quality control; Secondly, the quality examination after process reform or preparation pilot scale are amplified; The 3rd, new spec exempts to do the foundation of bioequivalence test; In addition,, calculate dosage and on the course of treatment, exploring new dosage, study aspects such as efficient, quick-acting, slowly-releasing sustained action dosage form, all play important effect for design of control pharmaceutical preparation quality prescription and screening.Pharmaceutical factory, medicine inspecting institute, clinical hospital, drug research and pharmacologic education unit all need to carry out this work.But China overwhelming majority units remain manual sample analysis operation so far, and not only trivial operations and the data that obtain are imperfect.
Because importance and the hard work amount of dissolution determination in Pharmaceutical Analysis.Abroad begin the seventies, and ' dissolution rate automatic analyzer ' becomes one of emphasis of big instrument company of many families research and development, if any the product of companies such as U.S. Beckman, Hanson, Vandeikamp.
Present widely used automatic analysis of dissolution instrument, it comprises self-actuated sampler or multiple tracks peristaltic pump, dissolving device, detecting instrument (visible-ultraviolet spectrophotometer, electrochemical detector or high performance liquid chromatograph) and computing machine etc.For example: Precision Instrument Factory, Tianjin Univ. " the automatic analyzing and testing of ADUV8 medicine stripping system ", it has realized constant temperature water bath control, controllable rotation speed, software can be pointed out sample stripping, release test, can also calculate, print stripping curve and test parameters automatically.General automatic dissolution rate monitor by " multitube peristaltic pump " test solution in the test glass is pumped into the UV-Vis spectrophotometer respectively or HPLC detects, and has reduced the miscellaneous work amount of hand sampling and the error that operation brings.But, its real-time monitoring on the throne of being unrealized, and have that filter easily stops up, trace drug absorption, easily produce bubble and measure problems such as cleaning amount in back is big.
Two, summary of the invention:
The invention provides a kind of fibre optic chemical sensor probe, it is applied to liquid or gaseous matter characteristic measurement, for the problem that solves above-mentioned existence has been done necessary preparation.
One of technical scheme of the present invention realizes by following measure: a kind of fibre optic chemical sensor probe, and it comprises jacket layer, optical fiber and sensitive membrane, the test side of optical fiber is equipped with sensitive membrane.The length of its optical fiber is that 25cm to 250cm, diameter are 10.0 μ m to 2500 μ m.It is applicable to makes miniature fibre optic chemical sensor probe.
Two of technical scheme of the present invention realizes by following measure: a kind of fibre optic chemical sensor probe, it comprises sleeve pipe, optical fiber and reflective mirror, the test side of optical fiber is installed in the inner end of sleeve pipe, outer end at sleeve pipe is equipped with reflective mirror, has at the middle part of sleeve pipe to be no less than one injection port.The length of its optical fiber is that 25cm to 250cm, diameter are 0.1cm to 2.50cm.Because its light path (being the distance between reflective mirror and the optical fiber test side) is specific, so it is applicable to the mensuration of making particular solid pharmaceutical preparation (tablet, capsule etc.) dissolution rate or release; That is: different solid pharmaceutical preparations are when needs carry out the mensuration of dissolution rate or release, and its light path is different; Thereby the fibre optic chemical sensor probe of the present invention of different light paths is applicable to the mensuration of different solid pharmaceutical preparations (tablet, capsule etc.) dissolution rate or release, therefore, this fibre optic chemical sensor probe is applicable to the fibre optic chemical sensor probe of making ad hoc type.
Three of technical scheme of the present invention realizes by following measure: a kind of fibre optic chemical sensor probe, it comprises sleeve pipe, optical fiber and reflective mirror, the test side of optical fiber is installed in the inner end of sleeve pipe, outer end at sleeve pipe is equipped with adjusting light path bar by engage thread or inserted mode, at the inner end of regulating the light path bar reflective mirror is installed, has at the middle part of sleeve pipe to be no less than one injection port.The length of its optical fiber is that 25cm to 250cm, diameter are 0.1cm to 2.50cm.Because its light path (being the distance between reflective mirror and the optical fiber test side) is adjustable, therefore it is applicable to the mensuration to different solid pharmaceutical preparations (tablet, capsule etc.) dissolution rate or release, thereby it can be used as universal fibre optic chemical sensor probe, and can adopt ultraviolet/visible/near infrared absorption, reflection and phosphorescence/fluorescence/light sources such as Raman spectrum radiation.
Can be coated with the ultraviolet reflectance film in the above-mentioned reflective mirror outside.
Test side at above-mentioned optical fiber can be equipped with the optical fiber focus lamp.
Above-mentioned optical fiber can adopt y-type optical fiber or branch type optical fiber or not branch type optical fiber or single-mode fiber or multimode optical fiber.
The present invention can be used for the on the throne real-time monitoring of liquid or gaseous matter characteristic measurement, be applied to especially solid pharmaceutical preparation (tablet, capsule etc.) mensuration of dissolution rate or release, can be made into and be applicable to miniature fibre optic chemical sensor probe, also can be made into and be applicable to and make particular solid pharmaceutical preparation (tablet, capsule etc.) fibre optic chemical sensor of the ad hoc type of dissolution rate or drug release determination probe, also can be made into universal fibre optic chemical sensor probe, and can adopt ultraviolet/visible/near infrared absorption, reflection and phosphorescence/fluorescence/light sources such as Raman spectrum radiation, and avoided stopping up in the mensuration of dissolution rate or release, trace drug absorption, easily produce the generation of problems such as bubble and mensuration back cleaning amount are big.
Four, description of drawings:
Accompanying drawing 1 is looked the sectional structure synoptic diagram for the master of the embodiment of the invention 1;
Accompanying drawing 2 is looked the sectional structure synoptic diagram for the master of the embodiment of the invention 2;
Accompanying drawing 3 is looked the sectional structure synoptic diagram for the master of the embodiment of the invention 3;
Accompanying drawing 4 is the main TV structure synoptic diagram of the embodiment of the invention 4, and accompanying drawing 5 is the side-looking sectional structure synoptic diagram of accompanying drawing 4;
Accompanying drawing 6 is the main TV structure synoptic diagram of the embodiment of the invention 5;
Coding in the accompanying drawing is respectively: 1 is jacket layer, and 2 is optical fiber, and 3 is sensitive membrane, 4 is the test side of optical fiber, and 5 is sleeve pipe, and 6 is reflective mirror, 7 is the inner end of sleeve pipe, and 8 is the outer end of sleeve pipe, and 9 is the inboard of reflective mirror, 10 is the middle part of sleeve pipe, 11 is injection port, and 12 for regulating the light path bar, and 13 for regulating the inner end of light path bar, 14 are the ultraviolet reflectance film, and 15 is the optical fiber focus lamp.
Five, embodiment:
The present invention is not subjected to the restriction of following embodiment, can determine concrete embodiment according to technical scheme of the present invention and actual conditions.
Below in conjunction with embodiment and accompanying drawing the present invention is further described:
Embodiment 1, as shown in Figure 1, this fibre optic chemical sensor probe comprises jacket layer 1, optical fiber 2 and sensitive membrane 3, the test side 4 of optical fiber is equipped with sensitive membrane 3, the length of its optical fiber 2 is that 25cm to 250cm, diameter are 10.0 μ m to 2500 μ m, can determine concrete length and diameter according to actual needs, it is applicable to makes miniature fibre optic chemical sensor probe.
Embodiment 2, as shown in Figure 2, this fibre optic chemical sensor probe comprises sleeve pipe 5, optical fiber 2 and reflective mirror 6, the test side 4 of optical fiber is installed in the inner end 7 of sleeve pipe 5, outer end 8 at sleeve pipe 5 is equipped with reflective mirror 6, at the middle part 10 of sleeve pipe 5 one injection port 11 is arranged, the length of its optical fiber 2 is that 25cm to 250cm, diameter are 0.1cm to 2.50cm, determines concrete length and diameter too according to actual needs; As shown in Figure 2, according to the mensuration of different solid pharmaceutical preparations being carried out dissolution rate or release, produce the fibre optic chemical sensor probe of different light paths, be specially adapted to mensuration, be applicable to the fibre optic chemical sensor probe of making ad hoc type particular solid pharmaceutical preparation (tablet, capsule etc.) dissolution rate or release.
Embodiment 3, and shown in accompanying drawing 2 and 3, embodiment 3 is with the difference of embodiment 2: two injection port 11 is arranged at the middle part 10 of sleeve pipe 5.
Embodiment 4, and shown in accompanying drawing 2,3,4 and 5, embodiment 4 is with the difference of embodiment 2: the injection port 11 that a big lateral opening is arranged at the middle part 10 of sleeve pipe 5.
Embodiment 5, shown in accompanying drawing 5 and 6, embodiment 5 is with the difference of embodiment 4: be equipped with by engage thread or inserted mode in the outer end 8 of sleeve pipe 5 and regulate light path bar 12, at the inner end 13 of regulating light path bar 12 reflective mirror 6 is installed, the injection port 11 of a big side opening is arranged at the middle part 10 of sleeve pipe 5.By regulating light path bar its light paths of 12 scalable (being the distance between reflective mirror and the optical fiber test side), thereby be applicable to mensuration to different solid pharmaceutical preparations (tablet, capsule etc.) dissolution rate or release, thereby it can be used as universal fibre optic chemical sensor probe, can adopt ultraviolet/visible/near infrared absorption, reflection and phosphorescence/fluorescence/light sources such as Raman spectrum radiation.
To detect performance in order further improving, as shown in Figure 6, sensitive membrane 3 can be installed in the inboard of reflective mirror 6; In order to be applicable to the detection of ultraviolet light, can be coated with ultraviolet reflectance film 14 in reflective mirror 6 outsides; In order to be applicable to more weak light source, optical fiber focus lamp 15 can be installed in the test side 4 of optical fiber.In order to increase adaptability, optical fiber can adopt y-type optical fiber or branch type optical fiber or not branch type optical fiber or single-mode fiber or multimode optical fiber.
The most preferred embodiment of the present invention that above technical characterictic has constituted, it has stronger adaptability and best implementation result, can increase and decrease non-essential technical characterictic according to actual needs.
Sensitive membrane among the present invention can adopt following three class disclosed methods to obtain respectively.
1. " molecular probe+polymkeric substance+plastifier " solvent evaporation method is made even sensitive membrane; Its disclosed document is:
[1] Seitz, WR., etc. based on the fibre optic chemical sensor of immobilizing indicator.Analytical chemistry, CRC Crit Rev, 1988; 19:135-139. (Seitz, WR., et al, Chemical Sensors based on immobilized indicatorsand fiber optic, Anal.Chem., CRC Crit Rev, 1988; 19:135-139.)
[2] W.Rudolt Seitz. fibre optic chemical sensor polymkeric substance indicator substrate.Biosensor technology. (W.RudoltSeitz.Polymeric Indicator Substrates for Fiber Optic Chemical Sensors.Biosensor Technology.)
[3] Kuit Seiler, Kemin Wang, Matthias Kuratli and Wilhelm Simon; A kind of ethanol selectivity optical fiber sensing membrane based on the reversible chemical identifying.The analytical chemistry journal, 1991; 244:151-1601. (Kuit Seiler, Kemin Wang, Matthias Kuratli and Wilhelm Simon; Development of an ethanol-selectiveoptode membrane based on a reversible chemical recognition process.Anal.Chim.Acta, 1991; 244:151-1601.)
[4] Otto S.Wolfbeis. chemical sensitisation indicator dye.Fibre optic chemical sensor, 1997,4:53-107. (Otto S.Wolfbeis.Chemical sensing using indicator dyes.Optical fiber sensor.1997,4:53-107.)
2. " sol-gel " legal system sensitive membrane (mesh is arranged, get rid of solid content disturb) in the sensitive membrane surface distributed, its disclosed document is:
[5] Rainer Klein, the ammoniacal sensor that Edgar Voges. optics is integrated. Dutch analytical chemistry magazine, (1994) 349:394-398. (Rainer Klein, Edgar Voges.Integrated-optic ammonia sensor.FreseniusJ Anal.Chem. (1994) 349:394-398.)
[6] David J.Blyth, Sarah J.Poynter and David A.Russell is with the fixing calcium biology sensor of photosensitive protein of sol-gal process.The analyst, 1996,12, vol.121 (1975-1978). (David J.Blyth, SarahJ.Poynter and David A.Russell, Calcium Biosensing with a Sol-gel Immobilized Photoprotein.Analyst, December 1996, vol.121 (1975-1978) .)
[7] R.C.Hughes, S.V.Patel, M.W.Jenkins, T.J.Boyle, T.J.Gardner and C.J.Brinker is based on collosol and gel porous membrane chemical catalysis sensor.(R.C.Hughes，S.V.Patel，M.W.Jenkins，T.J.Boyle，T.J.Gardner?and?C.J.Brinker，Thin?film?Porous?Membranes?Based?on?Sol-gelChemistry?for?Catalytic?Sensors.)
[8] the collosol and gel membrane technology prepares sulfate anion chemistry microsensor (Sulfate Anion-Sensing ChemicalMicrosensors Prepared by the Sol-gel Membrane Technology.)
[9] O.Lev, M.Tsionsky, L.Rabinovich, the collosol and gel sensor of et al. organic decoration.Analytical chemistry, 1995, Vol.67 (1): 22A-30A. (O.Lev, M.Tsionsky, L.Rabinovich, et al.Organicallymodified Sol-Gel Sensors.Analytical Chemistry, 1995, Vol.67 (1): 22A-30A.)
[10] Dave B.C., Dunn B., Valentine J.S.et al. sol-gel embedding legal system is equipped with biology sensor.Analytical chemistry, 1994,66 (22): 1120A-1127A. (Dave B.C., Dunn B., Valentine J.S.et al.Sol-Gelencapsulation methods for biosensors.Anal.Chem.[J], 1994,66 (22): 1120A-1127A.)
[11] Kwang E.Chung, Esther H.Lan, Michael S.Davidson. glass embedding myoglobin is measured oxygen in water.Analytical chemistry, 1995 (67): 1505-1509. (Kwang E.Chung, Esther H.Lan, MichaelS.Davidson.Measurement of dissolved oxygen in water using glass-encapsulatedMyoglobin.Anal.Chem.1995 (67): 1505-1509.)
[12] Upvan Narang, Paras n.Prasad, and Frank V.Bright. is based on the glucose biological sensor of collosol and gel derivatization.Analytical chemistry, 1994 (66): 3139-3144. (Upvan Narang, Paras n.Prasad, andFrank V.Bright.Glucose biosensor based on Sol-Gel-derived platform.Anal.Chem.1994 (66): 3139-3144.)
[13] Faida A.El-Essi, Ali Z.Abu Zuhri, Suleiman I.Al-Khalil, et al. is with the fixing hydrogen peroxide that produces of horseradish peroxidase spectrographic determination enzymatic of collosol and gel.Talanta，1997(44)2051-2058.(Faida?A.El-Essi，Ali?Z.Abu?Zuhri，Suleiman?I.Al-Khalil，et?al.Spectrophometricdetermination?of?enzymatically?generated?hydrogen?peroxide?using?Sol-gel?immobilizedhorseradish?peroxidase.Talanta，1997(44)2051-2058.)
[14] L.M.Ellerby, C.R.Nishida, S.A.Yamanka, the transparent porous silica glass of et.al. Prepared by Sol Gel Method embedding albumen.Science [J], 1992,225:1113-1115. (L.M.Ellerby, C.R.Nishida, S.A.Yamanka, et.al.Encapsulation of protein in transparent porous silicate glassesprepared by the sol-gel method.Science[J], 1992,225:1113-1115.)
3. the covalent bonding legal system is equipped with sensitive membrane, and its disclosed document is:
[15] the optical fiber fluorescence sensing method of evaluation antibody technique for fixing.The analytical chemistry journal, 229 (1990) 169-176. (Evaluation of antibody immobilization techniques for fiber optic-basedfluoroimmunosensing.Analytica Chimica Acta, 229 (1990) 169-176.)
[16] optical fiber biosensor of immobilized enzyme.Biosensor technique.(Fiber?Optic-Based?BiosensorsUtilizing?Immobilized?Enzyms.Biosensor?Technology.)
[17] Susan L.R.Barker, Yunde Zhao and Michael A.Marletta; Solubility guanine cyclase heme based on dye marker is applied to the highly sensitive of cell and high selectivity optical fiber nitrogen monoxide biology sensor.Analytical chemistry, 1999,71,2071-2075. (Susan L.R.Barker, Yunde Zhao and Michael A.Marletta; Cellular applications of a sensitive and selective fiber-optic Nitric Oxide biosensorbased on a dye-labeled heme domain of soluble guanylate cyclase.Anal.Chem.1999,71,2071-2075.)
[18] Julia Cordek, Xinwen Wang and Weihong Tan; Fibre-optical probe directly fixedly the glutamate dehydrogenasa be used for hypersensitive and measure glutamate.Analytical chemistry, 1999,71,1529-1533. (Julia Cordek, Xinwen Wangand Weihong Tan; Direct immobilization of Glutamate dehydrogenase on optical fiberprobes for ultrasensitive glutamate detection.Anal.Chem.1999,71,1529-1533.)
[19] Zhongping Chen, D.L.Kaplan and H.Gao; Molecule integrated multi-layer enzyme: be used to develop a kind of based on chemiluminescent optical fiber biosensor.Materials Science and Engineering, C4 (1996) 155-159. (Zhongping Chen, D.L.Kaplan and H.Gao; Molecular assembly of multiplayer enzyme:toward the developmentof a chemiluminescence-based fiber optic biosensor.Material Science and Engineering.C4 (1996) 155-159.)
[20] Frank Kleinjung, Krank F.Bier and Axel Warsinke; The optical fibre bio gas sensor is used for selectivity and measures nanomole DNA oligomer.The analytical chemistry journal, 350 (1997) 51-58. (Frank Kleinjung, KrankF.Bier and Axel Warsinke; Fibre-optic genosensor for specific determination offemtomolar DNA oligomer.Analytical Chimica Acta 350 (1997) 51-58.)
[21] M.A.Karymov, A.A.Kruchinin and Yu.A.Tarantov; The direct fixed dna in fibre-optic waveguide surface is used to measure the biology sensor of molecule.Sensor and actuator B29 (1995) 324-327. (M.A.Karymov, A.A.Kruchinin and Yu.A.Tarantov; Fixation of DNA directly on optical waveguide surfacesfor molecular probe biosensor development.Sensors and actuators B29 (1995) 324-327.)
More than the sensitive membrane of three kinds of methods preparation be applicable to the monitoring of various chemical constitutions in liquid or the gas respectively.
Principle of work of the present invention: the present invention is applicable to the process monitoring on the throne of mensuration " ultraviolet/visible/near infrared absorption, reflection and phosphorescence/fluorescence/raman radiation spectrum " respectively according to different analytic targets.
One, the measurement of ultraviolet/visible/near infrared absorption or reflectance spectrum radiation: optical signals optical fiber feeds back to detecting device or spectrophotometer, and output signal meets following mathematic(al) representation:
A = log l 0 l t = - log T = abC = K . C . L
a?or?K：E 1％、E 1‰?or?ε/λ max、1cm。C：g/100ml、g/L?or?mol/L
Two, the actinometry of fluorescent/phosphorescent and Raman spectrum: spectral signal feeds back to ultraviolet/visible/near infrared spectrometer or fluorospectrophotometer or detecting device by optical fiber, and signal output meets following mathematic(al) representation:
I(F、P?or?R)＝Φ 0×I 0×ε×L×C
I (F): fluorescence intensity, I (P): phosphorescence intensity, I (R): Raman light intensity, Φ: constant term, I 0: incident intensity, ε: absorptivity, L: light path, C: analyte concentration.
Spectral signal feeds back to detecting device or fluorescent/phosphorescent/Raman spectrometer by optical fiber, obtains fluorescence, phosphorescence or Raman spectrum.
Three, the measurement of the polynary quencher spectral radiance of fluorescence: in optical fiber end device sensitive membrane, accurate adjustable distance is arranged, form microcuvette.Analyte absorbs radiation (the first inner filtration IFE from light source in this space 1), weaken the exciting light that arrives sensitive membrane (reagent phase), the film emitted fluorescence is weakened.When the fluorescence spectrum of the ultraviolet-visible UV-VIS spectrum of analyte and sensitive membrane overlaps, also will absorb fluorescence (second inner filtration, the IFE of sensitive membrane 2), resonance energy takes place simultaneously shift (FTE) or dynamics quencher.Mathematic(al) representation:
Figure C20041000175500082
First is the dynamics quencher; Second is static quenching; The 3rd is the resonance energy transfer; The 4th is first inner filtration; The 5th is second inner filtration; F 0Be respectively quencher Q with F and add fluorescence intensity preceding and that the adding back is measured, [Q] is broad sense quencher concentration, τ 0Be fluorescence decay life-span, K QBe dynamics quenching constant, K SThe static quenching constant, E ExQuencher is to the molar absorptivity of exciting light, E EmQuencher is to radiative molar absorptivity, and b is the effective light path of light by the agent of slightly going out.Following formula calculates through multivariate linear model, obtains two and simplifies mathematic(al) representation:
F 0/ F=Kpq[Q]+C or log (F 0/ F)=Kpq[Q]+C
F 0Fluorescence intensity when/F exists for blank and analyte, Kpq claims apparent quenching constant, characterizes the sensitivity of response.C is an intercept.Because multifactorial compatible and addition has improved mensuration sensitivity.Chemical reaction does not take place based on the physical optics principle in response in the mensuration process, therefore have good reversibility and long-life.

Claims (15)

1. a fibre optic chemical sensor is popped one's head in, it is characterized in that comprising sleeve pipe, optical fiber and reflective mirror, the test side of described optical fiber is installed in the inner end of described sleeve pipe, in the outer end of described sleeve pipe described reflective mirror is installed, and has at the middle part of described sleeve pipe to be no less than one injection port.
2. a fibre optic chemical sensor is popped one's head in, it is characterized in that comprising sleeve pipe, optical fiber and reflective mirror, the test side of described optical fiber is installed in the inner end of described sleeve pipe, outer end at described sleeve pipe is equipped with adjusting light path bar by engage thread or inserted mode, inner end at described adjusting light path bar is equipped with described reflective mirror, has at the middle part of described sleeve pipe to be no less than one injection port.
3. fibre optic chemical sensor probe according to claim 1 and 2, the length that it is characterized in that described optical fiber is that 25cm to 250cm, diameter are 0.1cm to 2.50cm.
4. fibre optic chemical sensor probe according to claim 1 and 2 is characterized in that there is sensitive membrane the inboard of described reflective mirror.
5. fibre optic chemical sensor probe according to claim 3 is characterized in that there is sensitive membrane the inboard of described reflective mirror.
6. fibre optic chemical sensor probe according to claim 1 and 2 is characterized in that the described reflective mirror outside is coated with the ultraviolet reflectance film.
7. fibre optic chemical sensor probe according to claim 5 is characterized in that the described reflective mirror outside is coated with the ultraviolet reflectance film.
8. fibre optic chemical sensor probe according to claim 1 and 2 is characterized in that the test side of described optical fiber is equipped with the optical fiber focus lamp.
9. fibre optic chemical sensor probe according to claim 7 is characterized in that the test side of described optical fiber is equipped with the optical fiber focus lamp.
10. fibre optic chemical sensor probe according to claim 8 is characterized in that described optical fiber adopts branch type optical fiber.
11. fibre optic chemical sensor probe according to claim 9 is characterized in that described optical fiber adopts branch type optical fiber.
12. fibre optic chemical sensor probe according to claim 11 is characterized in that described branch type optical fiber adopts y-type optical fiber.
13 fibre optic chemical sensor probes according to claim 12 is characterized in that described y-type optical fiber adopts single-mode fiber or multimode optical fiber.
14. fibre optic chemical sensor probe according to claim 9 is characterized in that described optical fiber adopts not branch type optical fiber.
15 fibre optic chemical sensor probes according to claim 14 is characterized in that described not branch type optical fiber adopts single-mode fiber or multimode optical fiber.
CN 200410001755 2004-01-22 2004-01-22 Probe of fiber optic chemical sensor Expired - Fee Related CN1278116C (en)

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CN101696932B (en) * 2009-11-02 2012-12-19 中国计量学院 Optical fiber chemical sensor of over-long optical absorption action distance and preparation method thereof
CN104089879A (en) * 2013-04-01 2014-10-08 上海富科思分析仪器有限公司 Optical fiber-based drug dissolution rate and release rate process analyzer
CN106226205A (en) * 2016-08-22 2016-12-14 中国人民解放军理工大学 A kind of transition microbubble plume observation device and observation procedure
CN106334337B (en) * 2016-11-03 2018-12-18 天津中医药大学 A kind of traditional Chinese medicine extraction near infrared online detection function, concentration tank
CN107084981B (en) * 2017-06-21 2023-06-16 中国海洋大学 High-precision pH sensor based on nanomaterial sustained-release acid-base indicator photometry
CN113008838B (en) * 2021-03-17 2023-04-11 华北电力大学 Optical cavity, heating cavity, measuring device and liquid medium transmission spectrum measuring system

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