CN1276723C - Water soluble royal jelly and its dry powder and method for preparing them - Google Patents
Water soluble royal jelly and its dry powder and method for preparing them Download PDFInfo
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- CN1276723C CN1276723C CNB2004100174230A CN200410017423A CN1276723C CN 1276723 C CN1276723 C CN 1276723C CN B2004100174230 A CNB2004100174230 A CN B2004100174230A CN 200410017423 A CN200410017423 A CN 200410017423A CN 1276723 C CN1276723 C CN 1276723C
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Abstract
The present invention provides water-soluble royal jelly and dry powder thereof, and a preparation method of the water-soluble royal jelly and the dry powder. Proteins in the royal jelly can be dissolved into water by being hydrolyzed by one kind or more than one kind of proteinase. The present invention is characterized in that bitter hydrophobic polypeptide and acerbic and hot beta-cyclodextrin for 10-hydroxycaproleic acid and/or beta-cyclodextrin by chemical modification are embedded. The preparation method comprises the steps that homogenized slurry of the royal jelly is prepared; the proteins of the royal jelly are dissolved into water by the action of the proteinase at optimal pH value and temperature, then the mixture is heated to eliminate enzymes; the hydrophobic polypeptide, the 10-hydroxycaproleic acid embedded by cyclodextrin in a hydrolysis solution, etc. are added into the hydrolysis solution processed by eliminating enzymes. The product has the advantages of complete dissolution in the water, no bitter taste, physiological nutrient properties like fresh royal jelly, etc. and is particularly suitable for beverage and oral solutions.
Description
Technical field
The present invention relates to a kind of water-soluble bee milk solution, concentrate or dry powder and their preparation method, but be a kind of CL water, no bitter taste specifically and have water-soluble bee milk solution, concentrate or dry powder and their preparation method with the same physiology nutritive peculiarity of fresh royal jelly.
Background technology
Bee milk is the traditional natural nourishing good merchantable brand of China, just find that as far back as the Han dynasty bee milk is the jewellery that braces up energy and delay senility, modern medicine and nutrition are also extremely praised highly bee product, think that bright emperor's slurry is the edible pure natural fetus level food of unique Gong the mankind in the world, the mankind are had extremely strong alimentary health-care function and medical function.Contain multiple bioactive ingredients such as the plurality of enzymes of protein, aliphatic acid, amino acid, vitamin, mineral matter, flavone compound and needed by human body and hormone in the bee milk.Fresh bee milk generally is flaxen viscous material, acidity is 3.6～6, the content percentage of main matter is: water 66～70%, about protein 12.3%, about lipoid 4.2%, glucose about 5.8%, fructose about 5.6%, about sucrose 3%, and the 10-hydroxyl caproleic acid about 2%.Bee milk has regulates organism endocrine and metabolic function, promotes regeneration, prevention cardiovascular and cerebrovascular disease, radioresistance, all multiactions such as anticancer and broad-spectrum antiseptic.And the result of toxicological test shows, bee milk has no side effect as wholefood, and rat is injected 3000mg/kg every day is not all had obvious toxic and side effects.Integrate nutrition, health care, dietotherapy just because of bee milk, and safety non-toxic, its as the natural nourishing product at prevent disease, nourishing healthy and the effect aspect promoting longevity be subject to people's attention day by day.
Major country of production as bee milk, China has also carried out big quantity research in the production and the application facet of bee milk, and develop various bee milk goods, as freeze-dried royal jelly powder, tablet, capsule and oral liquid etc., but these products problems that have following two aspects: one, have the distinctive sour and astringent and acid of bee milk, make the consumer be difficult to accept more.Sour and astringent and the acid of bee milk mainly comes from the 10-hydroxyl caproleic acid in the bee milk, and the 10-hydroxyl caproleic acid is by peculiar in the bee milk, so also be often referred to as E-10-hydroxy-2-decylenic acid.E-10-hydroxy-2-decylenic acid is the peculiar composition in the bee milk, and it is a kind of white crystals, and fusing point is 58～59 ℃, and stable in properties is soluble in methyl alcohol, ethanol, chloroform and ether, is slightly soluble in acetone, is insoluble in water.In present known bee milk, contain in the very useful about 114 kinds of materials of health, E-10-hydroxy-2-decylenic acid is considered to the key substance that health is worked as a kind of material that multiple physiologically active is arranged.Just in view of the unique status of E-10-hydroxy-2-decylenic acid in bee milk and only peculiar, so its content is the leading indicator of weighing the bee milk inherent quality always by bee milk.The content of Japan's E-10-hydroxy-2-decylenic acid from i.e. regulation import bee milk in 1980 must not be lower than 1.9%.Therefore, good bee milk goods should have higher E-10-hydroxy-2-decylenic acid content, but its content height must influence its taste again, also may separate out with crystallization, this contradiction shows particularly outstandingly in aqueous goods such as beverage that contains bee milk and oral liquid, and does not see effective solution yet at present.Two, be rich in protein in the bee milk, but molecular weight is 40, protein more than 000 accounts for 18～24% of total protein, molecular weight is 10,000～40, and 000 protein accounts for 22～30% of total protein, these protein solubilities are poor, add when doing raw material in beverage or the oral liquid etc., become white suspension or separation easily to separate out, have a strong impact on the image appearance of product and consumer confidence level product quality.Owing to there is not effectively to solve the unstability of protein in the bee milk, the addition of bee milk is generally very low in the food and drink that contains bee milk of China's development or the oral liquid, with the stability of assurance product, thereby the performance of bee milk health care and the consumption of bee milk product have been influenced greatly.Another one guarantees that the measure of the stability of product is: add in these food and drinks or the oral liquid after with methods such as centrifugation or filtrations the protein in the bee milk being removed again, but also removed part or even whole E-10-hydroxy-2-decylenic acids in isolated protein, obviously its cost is the nutritive index that has seriously reduced bee milk; Secondly, because protein shows as a kind of thick material, its lock out operation is rather difficult; Once more, the bee milk of Huo Deing when it adds in beverages or the oral liquid in a large number, still can not avoid generating the phenomenon of white suspension or precipitation like this.In addition, Chinese patent 92110352.2 discloses " process for preparing transparent royal jelly solution ", it is to modulate the royal jelly aqueous suspension earlier, again to wherein simultaneously or add two kind or the above protease different successively to the matrix site of action, temperature keeps by enzyme reaction insoluble matter can being dissolved more than the room temperature.This method has significantly been improved the dissolubility of protein, but not overcoming bee milk yet has sour and astringent and this deficiency of acid, but also brought the new factor that influences taste, be that protein is easy to generate the material with bitter taste behind enzymolysis, for preventing the generation of bitter substance, need strictness to select protease and strict control enzymatic hydrolysis condition for use, even like this, the generation of bitter substance still is difficult to avoid, and production cost is also higher relatively.
Summary of the invention
First technical problem to be solved by this invention is to provide water-soluble bee milk solution, concentrate or the dry powder that is dissolvable in water water and does not have bitter taste at the prior art present situation.
Second technical problem to be solved by this invention is the preparation method that above-mentioned water-soluble bee milk solution, concentrate or dry powder are provided at the prior art present situation.
The present invention solves the technical scheme that above-mentioned first technical problem adopts: this water-soluble bee milk solution, concentrate or dry powder, protein wherein is through one or more protease hydrolytics and water soluble, the 10-hydroxyl caproleic acid that has the hydrophobicity polypeptide of bitter taste in the hydrolysate and have sour and astringent and acid has carried out embedding with beta-schardinger dextrin-and/or through the beta-schardinger dextrin-of chemical modification, the molecular weight distribution that it is characterized in that the protein behind protease hydrolytic is: molecular weight is 40, the percentage composition of component more than 000 in total protein is≤0.5%, molecular weight is 10,000～40, the percentage composition of 000 component in total protein is≤2.0%, molecular weight is 10,00～10, the percentage composition of 000 component in total protein is≤35.0%, molecular weight is 〉=60.0% at the percentage composition of the component below 1,000 in total protein.
Described beta-schardinger dextrin-through chemical modification is 2,6-DM-beta-schardinger dextrin-, hydroxypropyl, methyl beta-schardinger dextrin-or carboxymethyl beta-schardinger dextrin-.
The preparation method of the water-soluble bee milk solution of this kind, concentrate or dry powder, it is with fresh royal jelly or comprise that the various bee milk dried products of freeze-dried royal jelly powder are raw material, may further comprise the steps:
Produce the bee milk homogeneous slurry;
Hydrolysis; Regulate pH value to the adequate proteins enzyme of slurries with certain density alkaline solution (as sodium hydroxide solution or sodium carbonate etc.) or acid solution (example hydrochloric acid solution, citric acid etc.) and bring into play bioactive pH value, add protease, stir, regulate temperature to the adequate proteins enzyme of slurries and bring into play bioactive temperature, make the proteolysis in the bee milk become DDGS, hydrolysis time was generally 2～8 hours;
Enzyme goes out; After treating that nitrogen soluble index NSI in the hydrolyzate reaches 90%, will be heated to through the hydrolyzate that hydrolysis obtains more than 60 ℃, make the protease inactivation, stop hydrolysis; Preferably handle at 100 ℃ of enzymes that go out, the time is 5～8 minutes; In addition, preferably treat nitrogen soluble index NSI in the hydrolyzate reach more than 95% after heating again, to reach high degree of hydrolysis and to make product more as clear as crystal;
Embedding; Add consumption in the hydrolyzate of handling through the enzyme that goes out and be 1～6% beta-schardinger dextrin-of bee milk homogeneous slurry weight and/or through the beta-schardinger dextrin-of chemical modification, stir hydrophobicity polypeptide under 40～55 ℃ of temperature conditions in the embedding hydrolyzate and 10-hydroxyl caproleic acid; Temperature control in the described embedding treatment has a significant effect to the cyclodextrin consumption, finds after deliberation, and the heating post processing can reduce the cyclodextrin consumption, so the temperature of embedding treatment is advisable with 40～55 ℃.
So far, promptly obtain not having bitter taste, the water-soluble bee milk solution of sour and astringent and acid; Perhaps, further the concentrating of water-soluble bee milk solution promptly obtained water-soluble bee milk concentrate; Perhaps, concentrate is further carried out drying and pulverization process again, promptly obtain water-soluble bee milk dry powder.The common way that concentrates is to be concentrated into solid content and to reach 10～20% carrying out vacuum decompression below 50 ℃.In concentrate system dry powder, dry processing can be adopted freeze drying or vacuum drying, and is dry and behind water balance, pulverizes, and sieving promptly gets dry powder.
There is no particular restriction for described protease, comprise various plant source protein enzymes, animal-based protein enzyme and microbial source protease, as papain, bromelain, ginger protease, pepsin, trypsase, subtilopeptidase A, bacillus licheniformis protease etc.
The concentration of bee milk is 10～35wt% in the described bee milk homogeneous slurry.
The addition of described protease is 3～15 ‰ of a bee milk homogeneous slurry weight.
Among the present invention, when producing the bee milk homogeneous slurry, if be raw material with the fresh royal jelly, it produces process: with sieve about 20 orders fresh royal jelly is filtered earlier, to remove impurity such as petroleum tailings wherein, the bee milk after will filtering then carries out homogeneous to be handled, make its energy 100% by sieving about 60 orders, to reach the enough uniform and smooth of slurry, the clean water that adds 2～8 times of amounts in the last bee milk behind homogeneous dilutes, and stirs.If be raw material with bee milk dried products such as freeze-dried powders, then can be earlier with the suitable quantity of water dissolving, and then carry out aforesaid operations.
When hydrolytic process adopts more than one protease, can select to adopt to add simultaneously or add successively to be hydrolyzed according to the pH value and the temperature of the suitable hydrolysis of different protease.Under the more approaching situation of the suitable pH value of different protease and temperature, can add protease simultaneously and be hydrolyzed, so not only easy and simple to handle but also can obtain higher degree of hydrolysis.And differ under the big situation in the suitable pH value or the temperature of different protease, can add protease successively and be hydrolyzed.
For after the enzyme that obtains to guarantee the hydrolyzate of transparent clarification, be preferably in to go out handles, adopt centrifugation or filter method to remove the impurity and the insoluble matter that may contain in the hydrolyzate.
The bitter principle that studies show that protein hydrolyzate mainly is amino acid and derivative and low molecule bitter peptides such as leucine, methionine.If contacting just with the taste bud of human body, the amino of this part polypeptide can produce bitter taste.Though the content of bitter substance is atomic, very big to the influence of product special flavour, influenced the application of protein hydrolysate in industry greatly.
The principle of debitterize is the hydrophobicity polypeptide in the embedding hydrolysate.Beta-schardinger dextrin-by beta-schardinger dextrin-and chemical modification is to the research of bitter substance embedding effect; find they not only successfully embedding the bitter principle of royal jelly enzymolysis liquid; simultaneously the major function composition 10-hydroxyl caproleic acid in the royal jelly also there is the embedding effect; thereby not only protected the 10-hydroxyl caproleic acid better; and removed the distinctive acerbity of 10-hydroxyl caproleic acid; that has improved royal jelly widely is the flavor characteristic; make it can cooperate with other flavour of food products compositions better, be easier to be modulated into the Food ﹠ Drink of various different flavor types.Simultaneously, because they have solubilization to the embedding effect of 10-hydroxyl caproleic acid to 10-hydroxyl caproleic acid dissolubility in water, and improve the stability of 10-hydroxyl caproleic acid at product.
In addition, find that after deliberation along with the increase of enzyme dosage and the prolongation of enzymolysis time, the increase of hydrolyzate bitter taste is more remarkable, this is because the increase of enzyme dosage and enzymolysis time increases degree of hydrolysis on the one hand, causes the hydrolyzate bitter taste to strengthen; Be because the variation of E/S causes the selectivity variation of protease on the other hand, make the point of contact difference of its effect, bitterness intensity is also just different.Therefore, select suitable protease and suitable enzyme dosage and control suitable enzymolysis time and help obtaining the product that tool well is the characteristic of distinguishing the flavor of for use.
Because of protein has changed into the composition that is dissolvable in water water through protease hydrolytic, polypeptide and amino acid that more amount has the physiology function have been produced, and adopted the embedding techniques embedding and had the hydrophobicity polypeptide of bitter taste and a 10-hydroxyl caproleic acid with sour and astringent and acid, improved the water-soluble and stable of bee milk, improved its taste, therefore product of the present invention has following advantage: 1, can dissolve in all pH value scopes, can be used for the production of various beverage products, and manufactured goods are as clear as crystal, can not produce turbid phenomenon; 2, stable to heat treated, when heat treated and pasteurization processing, be difficult for producing precipitation; 3, difficult and polyphenols produces precipitation, can carry out compatibility with various tea beverage and drinks; 4, have better taste, obviously reduced the distinctive acerbity of bee milk, and the bitter taste that does not bring because of proteolysis; 5, easilier absorbed by human consumption; 6, easier preservation, more stable quality need not to carry out stored refrigerated as freeze-dried royal jelly powder.
The inventive method is except that the product of producing has as above advantage, and method itself has also that technology is simple, equipment needed thereby and equipment investment are few, be easy to realize producing in enormous quantities, production cost is low and the product yield advantages of higher.
The specific embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment one
Present embodiment is a raw material with the fresh royal jelly, is hydrolase with the pepsin, is the embedding thing with the cycloheptaamylose, and preparation process is as follows:
1, a certain amount of fresh royal jelly is filtered (20 mesh sieve), remove impurity such as petroleum tailings wherein.
2, the bee milk after will filtering carries out homogeneous and handles, make its can 100% by 60 mesh sieves, to reach the enough uniform and smooth of slurry.
3, the deionized water that adds 3 times of amounts in the bee milk behind homogeneous dilutes, and after stirring, the pH value to 2 with citric acid is regulated bee milk solution adds the pepsin that is equivalent to bee milk solution weight 7 ‰, stirs.
4, the temperature of regulating and keep solution is 40 ℃, hydrolysis 5 hours.
5, treat that nitrogen soluble index in the hydrolyzate (NSI) reaches 95% after, hydrolyzate is heated to 100 ℃ rapidly, the enzyme that keeps going out in 5～8 minutes is handled.Then, hydrolyzate is filtered, measure the bitterness value of hydrolyzate.The mensuration of nitrogen soluble index is as follows:
Get the 10ml enzymolysis liquid, leave standstill a period of time after transferring to different pH values, filter, with the soluble nitrogen in the triumphant formula nitriding mensuration supernatant, be calculated as follows nitrogen soluble index NSI then:
NSI (%)=(soluble nitrogen content/nitrogen pool) * 100
The bitter taste evaluation method:
With the 1ml sample be diluted to can just taste bitter taste till, its extension rate is bitterness value.The actual bitterness value that records hydrolyzate is 8.
6, the temperature to 50 of the hydrolyzate after regulate filtering ℃; in hydrolyzate, add 5% beta-schardinger dextrin-; after 30-40 minute stirring; hydrophobicity polypeptide (bitter principle) in the embedding hydrolyzate; slough the bitter taste of hydrolyzate; major function composition 10-hydroxyl caproleic acid in the royal jelly of embedding simultaneously, thus the 10-hydroxyl caproleic acid not only protected better, and removed the distinctive acerbity of 10-hydroxyl caproleic acid.Through practical measurement, nitrogen loss rate (%) is 0.
The mensuration of nitrogen yield: measure in the hydrolyzate nitrogen pool in the nitrogen pool and reaction system respectively, with percentage (%) the expression nitrogen yield of nitrogen pool in the hydrolyzate and the nitrogen pool in the reaction system.Wherein nitrogen analysis adopts micro-Kjeldahl.
After embedding treatment, regulating the pH value with sodium carbonate liquor is about 6, and the bitterness value that records solution is 0, thereby has obtained the water-soluble bee milk solution of no bitter taste, sour and astringent and acid.
Prepare concentrate as need, can reach 10～20% to solid content and get final product concentrating carrying out vacuum decompression below 50 ℃ through the hydrolyzate after the embedding treatment.
Further prepare dry powder as need, can adopt freeze drying, spray-drying or vacuum drying that concentrate is carried out drying and handle, after the drying, through water balance, pulverize, sieve (80 order) after the mixing, through being up to the standards, can be packed, and gets the dry powder finished product.
Embodiment two
Present embodiment is raw material with the fresh royal jelly, is hydrolase with alkaline bacillus licheniformis protease, and the protease consumption is 7 ‰, with the cycloheptaamylose is the embedding thing, and the deionized water addition is 4 times of amounts, and hydrolysis pH value is 8, temperature is 40 ℃, and the beta-schardinger dextrin-consumption is 2%, and all the other are with embodiment one.Actual recording, the bitterness value of hydrolyzate is 2, after embedding treatment, the bitterness value that records solution is 0.Nitrogen loss rate (%) is 0.
Embodiment three
Present embodiment is raw material with the fresh royal jelly, with alkaline subtilopeptidase A and alkaline bacillus licheniformis protease is hydrolase, the total consumption of protease is 7 ‰, with the cycloheptaamylose is the embedding thing, the deionized water addition is 4 times of amounts, and hydrolysis pH value is 8, and temperature is 40 ℃, the beta-schardinger dextrin-consumption is 2%, and all the other are with embodiment one.Actual recording, the bitterness value of hydrolyzate is 2, after embedding treatment, the bitterness value that records solution is 0.Nitrogen loss rate (%) is 0.
Embodiment four
Present embodiment is raw material with the fresh royal jelly, with trypsase and alkaline bacillus licheniformis protease is hydrolase, wherein the trypsase consumption is 5 ‰, alkalescence bacillus licheniformis protease consumption is 5 ‰, with carboxymethyl beta-schardinger dextrin-cycloheptaamylose is the embedding thing, the deionized water addition is 8 times of amounts, and earlier with trypsin hydrolysis, the pH value is 8, temperature is 40 ℃, hydrolysis time is 2 hours, and again with alkaline bacillus licheniformis protease hydrolytic, the pH value is 8, temperature is 40 ℃, hydrolysis time is 2 hours, and carboxymethyl beta-schardinger dextrin-consumption is 3%, and all the other are with embodiment one.Actual recording, the bitterness value of hydrolyzate is 7, after embedding treatment, the bitterness value that records solution is 0.Nitrogen loss rate (%) is 0.
Embodiment five
Present embodiment is raw material with the fresh royal jelly, with the pepsin is hydrolase, the protease consumption is 15 ‰, is the embedding thing with the hydroxypropyl, and the deionized water addition is 2 times of amounts, hydrolysis pH value is 2, temperature is 40 ℃, and hydrolysis time is 8 hours, and the hydroxypropyl consumption is 6%, the embedding treatment temperature is 40 ℃, and all the other are with embodiment one.Actual recording, the bitterness value of hydrolyzate is 11, after embedding treatment, the bitterness value that records solution is 0.Nitrogen loss rate (%) is 0.
Embodiment six
Present embodiment is raw material with the freeze-dried royal jelly powder, with the suitable quantity of water dissolving, be hydrolase with the papain earlier, the protease consumption is 8 ‰, with the carboxymethyl beta-schardinger dextrin-is the embedding thing, the deionized water addition is 2 times of amounts, and hydrolysis pH value is 8, and temperature is 40 ℃, hydrolysis time is 4 hours, carboxymethyl beta-schardinger dextrin-consumption is 3%, and the embedding treatment temperature is a normal temperature, and all the other are with embodiment one.Actual recording, the bitterness value of hydrolyzate is 5, after embedding treatment, the bitterness value that records solution is 0.Nitrogen loss rate (%) is 0.
Embodiment seven
Present embodiment is raw material with the freeze-dried royal jelly powder, dissolve with suitable quantity of water earlier, with alkaline bacillus licheniformis protease is hydrolase, and the protease consumption is 3 ‰, is the embedding thing with the carboxymethyl beta-schardinger dextrin-, the deionized water addition is 5 times of amounts, hydrolysis pH value is 8, and temperature is 40 ℃, and hydrolysis time is 2 hours, carboxymethyl beta-schardinger dextrin-consumption is 1%, and all the other are with embodiment one.Actual recording, the bitterness value of hydrolyzate is 2, after embedding treatment, the bitterness value that records solution is 0.Nitrogen loss rate (%) is 0.
After tested, the molecular weight of protein obviously reduces behind protease hydrolytic, and the distribution results of its molecular weight sees the following form:
| Divided amount size (dalton) | Fresh royal jelly | Water-soluble bee milk |
| ＞40,000 | 18-24％ | ≤0.5％ |
| 10,000-40,000 | 22-30％ | ≤2.0％ |
| 1,000-10,000 | 5-10％ | ≤35.0％ |
| ＜1,000 | 40-50％ | ≥60.0％ |
As seen from the above table, the macromolecule in the fresh royal jelly (more than 10,000) protein content accounts for the 40-54% of total protein content,, the macro-molecular protein of this part has influenced the processing characteristics of bee milk just.Water-soluble bee milk, molecular weight significantly improves less than 10,000 daltonian peptides and amino acid content, accounts for more than 95% of total protein content, thereby has improved the water-soluble of bee milk protein, simultaneously, it is easilier absorbed by human consumption.
Claims (4)
1, a kind of water-soluble bee milk solution, concentrate or dry powder, protein wherein is through one or more protease hydrolytics and water soluble, the 10-hydroxyl caproleic acid that has the hydrophobicity polypeptide of bitter taste in the hydrolysate and have sour and astringent and acid has carried out embedding with beta-schardinger dextrin-and/or through the beta-schardinger dextrin-of chemical modification, the molecular weight distribution that it is characterized in that the protein behind protease hydrolytic is: molecular weight is 40, the percentage composition of component more than 000 in total protein is≤0.5%, molecular weight is 10,000～40, the percentage composition of 000 component in total protein is≤2.0%, molecular weight is 10,00～10, the percentage composition of 000 component in total protein is≤35.0%, molecular weight is 〉=60.0% at the percentage composition of the component below 1,000 in total protein.
2, water-soluble bee milk solution according to claim 1, concentrate or dry powder is characterized in that described beta-schardinger dextrin-through chemical modification is 2,6-DM-beta-schardinger dextrin-, hydroxypropyl, methyl beta-schardinger dextrin-or carboxymethyl beta-schardinger dextrin-.
3, the preparation method of a kind of water-soluble bee milk solution, concentrate or dry powder, it is with fresh royal jelly or comprise that the various bee milk dried products of freeze-dried royal jelly powder are raw material, may further comprise the steps:
Produce the bee milk homogeneous slurry, the concentration of bee milk is 10～35wt% in the bee milk homogeneous slurry;
Hydrolysis; Regulate pH value to the adequate proteins enzyme of slurries and bring into play bioactive pH value, add protease, the addition of protease is 3～15 ‰ of a bee milk homogeneous slurry weight, stir, regulate temperature to the adequate proteins enzyme of slurries and bring into play bioactive temperature, make the proteolysis in the bee milk become DDGS;
Enzyme goes out; After treating that nitrogen soluble index NSI in the hydrolyzate reaches 90%, will be heated to through the hydrolyzate that hydrolysis obtains more than 60 ℃, make the protease inactivation, stop hydrolysis;
Embedding; Add consumption in the hydrolyzate of handling through the enzyme that goes out and be 1～6% beta-schardinger dextrin-of bee milk homogeneous slurry weight and/or through the beta-schardinger dextrin-of chemical modification, stir hydrophobicity polypeptide under 40～55 ℃ of temperature conditions in the embedding hydrolyzate and 10-hydroxyl caproleic acid;
So far, promptly obtain not having bitter taste, the water-soluble bee milk solution of sour and astringent and acid; Perhaps, further the concentrating of water-soluble bee milk solution promptly obtained water-soluble bee milk concentrate; Perhaps, concentrate is further carried out drying and pulverization process again, promptly obtain water-soluble bee milk dry powder.
4, the preparation method of water-soluble bee milk solution according to claim 3, concentrate or dry powder, it is characterized in that described protease comprises various plant source protein enzymes, animal-based protein enzyme and microbial source protease, the plant source protein enzyme comprises papain, bromelain, ginger protease, and the animal-based protein enzyme comprises pepsin, trypsase.
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| CNB2004100174230A CN1276723C (en) | 2004-04-02 | 2004-04-02 | Water soluble royal jelly and its dry powder and method for preparing them |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2007039402A (en) * | 2005-08-05 | 2007-02-15 | Taisho Pharmaceut Co Ltd | Liquid composition |
| CN102210373B (en) * | 2011-04-08 | 2013-01-23 | 中国农业大学 | Microencapsulated protein polypeptide and preparation method thereof |
| CN103315203B (en) * | 2012-03-20 | 2015-06-03 | 维他露食品股份有限公司 | Royal jelly solution and preparation method thereof |
| CN102670730B (en) * | 2012-05-17 | 2013-12-11 | 杭州天厨蜜源保健品有限公司 | Process for producing royal jelly extract lyophilized powder |
| JP6578133B2 (en) * | 2015-05-25 | 2019-09-18 | フロイント産業株式会社 | Enzymatically decomposed royal jelly powder composition and method for producing the same, tablet containing the enzymatically decomposed royal jelly powder composition, and method for improving flavor of enzymatically decomposed royal jelly |
| WO2018056123A1 (en) * | 2016-09-26 | 2018-03-29 | 株式会社山田養蜂場本社 | Stable enzyme-decomposed royal jelly granules with improved taste |
| CN115531438B (en) * | 2022-09-26 | 2024-03-12 | 岭南中药饮片有限公司 | Fructus psoraleae formula particles and preparation method thereof |
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