CN1256308C - Yew genus plant extract and its extraction method and application - Google Patents
Yew genus plant extract and its extraction method and application Download PDFInfo
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- CN1256308C CN1256308C CN 200410043649 CN200410043649A CN1256308C CN 1256308 C CN1256308 C CN 1256308C CN 200410043649 CN200410043649 CN 200410043649 CN 200410043649 A CN200410043649 A CN 200410043649A CN 1256308 C CN1256308 C CN 1256308C
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to taxus plant extract, an extraction method for the extract and an application thereof, particularly to plant extract, an extraction technology thereof and an application to anti-cancer medicine. The taxus plant extract of the present invention is extracted by using branches and leaves of northeast yew as raw materials, and comprises the components of taxane diterpene compounds, total lignanoid and total flavonoid. After branches and leaves of yew are concentrated and extracted, the taxus plant extract is obtained through pressure reduction and concentration, and the extract can be used as active components of anti-cancer medicine. The present invention is novel medicine prepared by medical sites of taxus plants, and contains various anti-cancer active components. The present invention has the characteristics of high yield, low cost, etc., and the anti-cancer effect is as good as paclitaxel. Meanwhile, the continuous utilization of taxus plants is realized, and the present invention has the advantage of low side effect. The utilization rate is improved by more than 25 times on the premise that therapeutic effect is ensured.
Description
Technical field:
The present invention relates to a kind of plant milk extract and extraction process thereof and as the application of cancer therapy drug, be specifically related to a kind of effective constituent of the cancer therapy drug that from Chinese yew genus plants is pruned the branches and leaves that get off, extracts and extraction process thereof and as the application of cancer therapy drug.
Background technology:
The patient that China dies from cancer every year increases day by day, and cancer has become one of disease of current social serious harm HUMAN HEALTH.The taxol that extracts from Chinese yew genus plants is used for the treatment of cancer, Clinical Practice proof determined curative effect.But the extraction of taxol is mainly derived from the bark of Chinese yew genus plants, and content is extremely low, and the result causes death, the extinction of Chinese yew genus plants.Disclose a kind of " Chinese yew genus plants extract and application thereof " among the CN1112433A, contained taxol in this extract, taxol is bigger as its side effect of drug use, and general patient is difficult to bear.
Summary of the invention:
The purpose of this invention is to provide the extracting method and the application thereof of a kind of Chinese yew genus plants extract and this extract, it had both solved the problem of Chinese yew genus plants continuous utilization, again under the prerequisite that guarantees curative effect, improved the utilization ratio of Chinese yew genus plants, brought up to more than 5/1000ths from single extraction taxol utilization ratio less than 2/10000ths.Chinese yew genus plants of the present invention is that the branches and leaves with taxus chinensis in northeast are that raw material extracts and forms, it comprises following compositions: Taxan diterpene compound, total lignans, total flavones, described Taxan diterpene compound account for three kinds of composition cumulative volumes 18～68%, total lignans account for three kinds of composition cumulative volumes 14～61%, total flavones accounts for 10～50% of three kinds of composition cumulative volumes.Its extracting method is prepared according to following step: a, be that 40～95% alcohol solvent returns and carries 1～3 hour with the branches and leaves volumetric concentration of Chinese yew genus plants medicinal material, be condensed into former medicinal extract; B, add the distilled water of 30～80 times of former medicinal extract weight then, the former medicinal extract of dissolving under 60～80 ℃ or ultransonic condition leaves standstill centrifugal, the precipitation of after-filtration; C, to be 50～90% ethanol with volumetric concentration extract above-mentioned throw out under 60～80 ℃ or ultransonic condition, extract three times, and the each add-on of ethanol is 5～15 times of weight of precipitate, leaves standstill after-filtration, centrifugal; D, merge ethanol extract, in extracting solution, add ethanol, be mixed with and contain that the alcohol amount is 20～40%, volume is the solution of 30～70 times of above-mentioned former medicinal extract; E, with above-mentioned solution by the macroporous adsorption resin chromatography post, the consumption of macroporous adsorbent resin is 10～20 times of former medicinal extract weight; F, be 20～40% ethanolic soln upper prop, wash to look light that the ethanol consumption is 8～12 times of column volume with volumetric concentration, flow velocity 0.5～1.5 column volume/hour; G, be 40～60% ethanolic soln upper prop with volumetric concentration then, wash to look light that the ethanol consumption is 8～15 times of column volume, flow velocity 0.5～1.5 column volume/hour; H, be 65～80% ethanolic soln upper prop with volumetric concentration again, wash to look light that the ethanol consumption is 10～15 times of column volume, flow velocity 0.5～1.5 column volume/hour; I, collect elutriant respectively, be evaporated to driedly, obtain the extract of Chinese yew genus plants.This extract can be used as the effective constituent of cancer therapy drug.When extract of the present invention used as the effective constituent of cancer therapy drug, this medicine can be processed into various formulations according to ordinary method in the pharmacy field, as injection, tablet, capsule etc.Chinese yew genus plants extract of the present invention is to be that feed purification forms with the taxus chinensis in northeast, Ramulus et folium taxi cuspidatae has anti-cancer and kill cancer action, advolution that can anticancer, can also improve simultaneously the circulation and the metabolic function of human body, make to be accumulated in the intravital virus of people and to be scavenged into externally by diuresis, defecation, reach the effect of preventing cancer, to the particularly postoperative patient of patient of cancer, can reduce the side effect of radiotherapy chemotherapy, play the effect of assisting therapy.Chinese yew genus plants extract of the present invention is a main component with Taxan diterpene compound, total lignans and total flavones, wherein the Taxan diterpene compound has unique antitumor action, it can combine the stable underwater pipeline stabilizing bundle of formation with tubulin and make by depolymerization, cancer cells is stopped at G2 late period or M phase, suppress cellular replication, stop cancer cells Z propagation; Total lignans has antineoplastic activity; Flavones has antioxygenation (removing free radical) and to the influence of multiple bioenzyme activity; Behind Taxan diterpene compound, total lignans and the total flavones compatibility, not only improve the curative effect of medicine, and had the effect that improves body immunity.Drug main of the present invention will be used for the treatment of lung cancer, melanoma, mammary cancer etc.It is obvious that this medicine is taken effect to postoperative patient, alternative or cooperate put, chemotherapy treats, the improvement of patient's lung cancer symptom is mainly shown on cough-relieving, hemostasis, the analgesic effect, and effect is remarkable.Simultaneously patient's appetite, physique etc. also there is significant improvement.The present invention is the medicine that utilizes the new medicinal part of Chinese yew genus plants branches and leaves to make, it contains multiple anticancer effective ingredient, has characteristics such as yield height, cost be low, and its anticancer effect is not less than taxol, realized simultaneously the continuous utilization of Chinese yew genus plants again, and side effect is extremely low.Under the prerequisite that guarantees curative effect, improved the utilization ratio of Chinese yew genus plants, to bring up to more than 5/1000ths from single extraction taxol utilization ratio less than 2/10000ths, utilization ratio improves more than 25 times.
Embodiment:
Embodiment one: the Chinese yew genus plants extract of present embodiment is that the branches and leaves with taxus chinensis in northeast are that raw material extracts and forms, and it comprises following compositions: Taxan diterpene compound, total lignans, total flavones.Described Taxan diterpene compound account for three kinds of composition cumulative volumes 18～68%, total lignans account for three kinds of composition cumulative volumes 14～61%, total flavones accounts for 10～50% of three kinds of composition cumulative volumes.Described Taxan diterpene compound comprises taxinine, taxinine B, 10-deacetylate baccatin III, 10-deacetylate taxinine B and Japanese yew cloud booth A; Described total lignans comprises conidendrin; Described total flavones comprises kaempferol and Naringenin.
Embodiment two: the Chinese yew genus plants extract of present embodiment is prepared according to following step: a, be that 40～95% alcohol solvent returns and carries 1～3 hour with the branches and leaves volumetric concentration of Chinese yew genus plants medicinal material, be condensed into former medicinal extract; B, add the distilled water of 30～80 times of former medicinal extract weight then, the former medicinal extract of dissolving under 60～80 ℃ or ultransonic condition leaves standstill centrifugal, the precipitation of after-filtration; C, to be 50～90% ethanol with volumetric concentration extract above-mentioned throw out under 60～80 ℃ or ultransonic condition, extract three times, and the each add-on of ethanol is 5～15 times of weight of precipitate, leaves standstill after-filtration, centrifugal; D, merge ethanol extract, in extracting solution, add ethanol, be mixed with and contain that the alcohol amount is 20～40%, volume is the solution of 30～70 times of above-mentioned former medicinal extract; E, with above-mentioned solution by the macroporous adsorption resin chromatography post, the consumption of macroporous adsorbent resin is 10～20 times of former medicinal extract weight; F, be 20～40% ethanolic soln upper prop, wash to look light that the ethanol consumption is 8～12 times of column volume with volumetric concentration, flow velocity 0.5～1.5 column volume/hour; G, be 40～60% ethanolic soln upper prop with volumetric concentration then, wash to look light that the ethanol consumption is 8～15 times of column volume, flow velocity 0.5～1.5 column volume/hour; H, be 65～80% ethanolic soln upper prop with volumetric concentration again, wash to look light that the ethanol consumption is 10～15 times of column volume, flow velocity 0.5～1.5 column volume/hour; I, collect elutriant respectively, be evaporated to dried, obtain the extract of Chinese yew genus plants, promptly as the effective constituent of cancer therapy drug, yield is 20～70%, and wherein the yield of each composition is: Taxan diterpene compound 8～25%, total lignans 7～20%, total flavones 5～15%, and mentioned component is through infrared, ultraviolet, nucleus magnetic resonance, mass spectrometric detection, its structural formula is:
Taxinine Japanese yew cloud booth A
Taxinine B 10-deacetylate baccatin III
10-deacetylate taxinine B kaempferol
The conidendrin Naringenin
Embodiment three: the Chinese yew genus plants extract of present embodiment is as the effective constituent of the cancer therapy drug of treatment lung cancer, melanoma, mammary cancer.
The pharmacodynamic experiment summary:
One, the standard of case selection:
1, lung cancer 40 examples, the male sex's 22 examples wherein, women's 18 examples, at minimum 29 years old of age, maximum 55 years old, 43 years old mean age, the course of disease is the shortest 2 years, and was the longest 10 years.
2, mammary cancer 32 examples are the women, and at minimum 33 years old of age, maximum 54 years old, 44 years old mean age, the course of disease is the shortest 1 year, and was the longest 11 years.
3, melanoma 25 examples, the male sex's 20 examples wherein, women's 5 examples, at minimum 44 years old of age, maximum 62 years old, 50 years old mean age, the course of disease is the shortest 5 years, and was the longest 20 years.
Two, the Case definition of disease:
1, lung cancer: cough, sputum mixed with blood, pectoralgia, shortness of breath.
2, mammary cancer: pain or sensation one or both sides breast turgor, nipple discharge.
3, melanoma: the melanochrome tubercle of growing up rapidly.
Three, the contained composition of medication: the injection of making by Chinese yew genus plants extract (500mg).
Four, administrated method: 7～10 days is a course of treatment, and inject once every day.
Five, the judging criterion of curative effect:
1, recovery from illness: symptom completely dissolve.
2, produce effects: symptom is clearly better.
3, effective: symptom turns for the better by and large.
4, invalid: symptom does not change.
Six, efficacy result analysis:
| Case | The example number | Recovery from illness (%) | Produce effects (%) | Effectively (%) | Invalid (%) | Total effective rate (%) |
| Lung cancer | 40 | 16(40) | 8(20) | 4(10) | 12(30) | 70 |
| Mammary cancer | 32 | 12(37.5) | 8(25) | 4(12.5) | 8(25) | 75 |
| Melanoma | 25 | 10(40) | 4(16) | 3(12) | 8(32) | 68 |
The pharmacodynamics preliminary study is found to answer Chinese yew genus plants extract to reach 68% as the curative effect of the application of cancer therapy drug under the situation of 125mg/kg, does not see any toxicity, and its therapeutic index is greater than taxol, and effectively the toxicity under the situation is starkly lower than taxol.
Claims (6)
1, Chinese yew genus plants extract, it is characterized in that it is that branches and leaves with taxus chinensis in northeast are that feed purification forms, it comprises following compositions: Taxan diterpene compound, total lignans, total flavones, wherein: the Taxan diterpene compound account for three kinds of composition cumulative volumes 18～68%, total lignans account for three kinds of composition cumulative volumes 14～61%, total flavones accounts for 10～50% of three kinds of composition cumulative volumes.
2, Chinese yew genus plants extract according to claim 1 is characterized in that described Taxan diterpene compound comprises taxinine, taxinine B, 10-deacetylate baccatin III, 10-deacetylate taxinine B and Japanese yew cloud booth A.
3, Chinese yew genus plants extract according to claim 1 is characterized in that described total lignans comprises conidendrin.
4, Chinese yew genus plants extract according to claim 1 is characterized in that described total flavones comprises kaempferol and Naringenin.
5, the extracting method of Chinese yew genus plants extract is characterized in that it is prepared according to following step: a, be that 40～95% alcohol solvent returns and carries 1～3 hour with the branches and leaves volumetric concentration of Chinese yew genus plants medicinal material, be condensed into former medicinal extract; B, add the distilled water of 30～80 times of former medicinal extract weight then, the former medicinal extract of dissolving under 60～80 ℃ or ultransonic condition leaves standstill centrifugal, the precipitation of after-filtration; C, to be 50～90% ethanol with volumetric concentration extract above-mentioned throw out under 60～80 ℃ or ultransonic condition, extract three times, and the each add-on of ethanol is 5～15 times of weight of precipitate, leaves standstill after-filtration, centrifugal; D, merge ethanol extract, in extracting solution, add ethanol, be mixed with and contain that the alcohol amount is 20～40%, volume is the solution of 30～70 times of above-mentioned former medicinal extract; E, with above-mentioned solution by the macroporous adsorption resin chromatography post, the consumption of macroporous adsorbent resin is 10～20 times of former medicinal extract weight; F, be 20～40% ethanolic soln upper prop, wash to look light that the ethanol consumption is 8～12 times of column volume with volumetric concentration, flow velocity 0.5～1.5 column volume/hour; G, be 40～60% ethanolic soln upper prop with volumetric concentration then, wash to look light that the ethanol consumption is 8～15 times of column volume, flow velocity 0.5～1.5 column volume/hour; H, be 65～80% ethanolic soln upper prop with volumetric concentration again, wash to look light that the ethanol consumption is 10～15 times of column volume, flow velocity 0.5～1.5 column volume/hour; I, collect elutriant respectively, be evaporated to driedly, obtain the extract of Chinese yew genus plants.
6, the described Chinese yew genus plants extract of claim 1 is as the application of the effective constituent of the cancer therapy drug of treatment lung cancer, melanoma, mammary cancer.
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| CN 200410043649 CN1256308C (en) | 2004-06-23 | 2004-06-23 | Yew genus plant extract and its extraction method and application |
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| CN 200410043649 CN1256308C (en) | 2004-06-23 | 2004-06-23 | Yew genus plant extract and its extraction method and application |
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| CN1256308C true CN1256308C (en) | 2006-05-17 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101230053B (en) * | 2007-12-04 | 2010-06-02 | 北京诺瑞医药技术有限公司 | Method for ultrasonic extraction and separation of 10-deacetyl baccatin III from yew branch leaves |
| CN103191041A (en) * | 2013-04-18 | 2013-07-10 | 江苏红豆杉药业有限公司 | Chinese yew branch and leaf extractive with anti-oxidative effect as well as extraction method and application of yew branch and leaf extractive |
| CN104140689B (en) * | 2014-07-14 | 2016-02-17 | 红豆集团无锡南国企业有限公司 | The method of natural pigment is extracted from Ramulus et folium taxi cuspidatae pharmacy residue |
| CN106389871A (en) * | 2016-08-31 | 2017-02-15 | 徐英奎 | Cancer-prevention compound traditional Chinese medicine composition comprising active taxus chinensis, and preparation method for composition |
| CN106421553A (en) * | 2016-08-31 | 2017-02-22 | 徐英奎 | Paclitaxel detoxified compound extract with anti-cancer effect and method for preparing paclitaxel detoxified compound extract |
| CN107056800B (en) * | 2017-05-31 | 2019-05-10 | 遵义医学院 | Dimer flavone constituents and its separation method and application in a kind of Tsuga longibracteata |
| CN107028934A (en) * | 2017-06-02 | 2017-08-11 | 广州中医药大学 | Application of the Kaempferol in anti-lung-cancer medicament is prepared |
| CN107137434B (en) * | 2017-06-22 | 2021-04-30 | 无锡紫杉药业有限公司 | Pharmaceutical composition, preparation method thereof and application thereof in preparation of coxsackie virus resistant medicine |
| CN107282010A (en) * | 2017-07-03 | 2017-10-24 | 安徽天顺环保设备股份有限公司 | A kind of heavy metal absorbent |
| CN111110709A (en) * | 2019-12-04 | 2020-05-08 | 田大伟 | Composition containing taxus chinensis extract and preparation method and application thereof |
-
2004
- 2004-06-23 CN CN 200410043649 patent/CN1256308C/en not_active Expired - Lifetime
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