CN1254544C - Method for producing hydrogen gas using steam cracked plant fermentation straw - Google Patents
Method for producing hydrogen gas using steam cracked plant fermentation straw Download PDFInfo
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- CN1254544C CN1254544C CN 02148681 CN02148681A CN1254544C CN 1254544 C CN1254544 C CN 1254544C CN 02148681 CN02148681 CN 02148681 CN 02148681 A CN02148681 A CN 02148681A CN 1254544 C CN1254544 C CN 1254544C
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Abstract
The present invention relates to a method for preparing hydrogen by steam explosion plant straw fermentation. In a semi solid sate fermentation reaction vessel, steam explosion straw raw materials, corn flour, beef peptone and water are proportionally and uniformly mixed; the mixed materials orderly carry out germicidal treatment, bacterium suspension vaccination and fermentation treatment to prepare hydrogen. The method has the step of collecting the prepared hydrogen by a hydrogen collection method. Because the adopted raw materials are steam explosion plant straws, the present invention has the advantages of abound resources and low price, effectively solves the problem of insufficient major biological hydrogen production raw materials caused by limited coal resources of organic waste water, and simultaneously overcomes the defects of low hydrogen yield, secondary environment pollution, etc. in the biological hydrogen production technology by organic waste water.
Description
Invention field
The invention belongs to the preparation method field of hydrogen as energy source, particularly a kind of method of using steam puffed plant straw fermenting process of preparing hydrogen.
Background technology
Energy and environment are most important themes in the human social process.In modern age, the human main energy sources of using has experienced the development of material firewood, coal, oil and natural gas, nuclear energy.Yet, still account for the fossil oil of maximum specific weight, produce a large amount of objectionable impuritiess, bring serious environmental to pollute; Simultaneously, along with world economy develop rapidly and population expansion increase, the energy consumption exponential growth makes fossil energy day by day deficient.So, clean new forms of energy have appearred again, as sun power, wind energy, Geothermal energy etc. and Hydrogen Energy.
Hydrogen Energy has incomparable advantage as a kind of energy of uniqueness.At first, the energy transformation ratio height of Hydrogen Energy can not be subjected to the restriction of carnot's working cycle by fuel cell, efficiently is converted into electric energy; Secondly, be the cleanliness of Hydrogen Energy, it can drop to minimum level to the harm to environment in the energy conversion process; The 3rd, Hydrogen Energy have can store up, the property failed.Hydrogen can obtain by fossil energy, also can obtain from biomass.
Hydrogen is produced mainly as a kind of intermediates at present.It is the important source material of further chemical industry hydrogeneration processes, mainly is by chemical industry energy gas (CH
4), crude oil and coal as raw material and water vapour at high temperature reform, gasification or hydro carbons partial oxidation transform and generate.Also emit CO by fossil energy hydrogen manufacturing
2Hydrogen also can be by brine electrolysis production, but this is a kind of method of costliness, and general by product under special production purpose is as chlorine industry.Need consume more steam with the hydrogen manufacturing of coal raw material, energy consumption height (coal lacks than oil, Sweet natural gas hydrogen content).At present, produce all large-scale productions of hydrogen from fossil energy, its output accounts for 96% of hydrogen ultimate production, and technology is comparatively ripe.Yet relying on traditional fossil energy, to come synthesis clean Hydrogen Energy and Sustainable development be conflicting.
Biological hydrogen production has a great deal of practical meanings to the application of real realization Hydrogen Energy, has been subjected to unprecedented attention since the nineties, and some developed countries have formulated the evolutionary operation(EVOP) of biological hydrogen production, in the hope of realizing the bio-hydrogen production technology industrialization as early as possible.Mainly be to adopt anaerobic bacterium to utilize organic waste water ferment for hydrogen production Study on Technology at present both at home and abroad.But it is low to exist factor of created gase, is difficult to satisfy the requirement of producing; Produce secondary pollution, lack supporting technology.Because the organic waste water stock number is limited, can not bear the main raw material of biological hydrogen production.
Summary of the invention
The objective of the invention is to overcome the many defectives that exist in the above-mentioned organic waste water bio-hydrogen production technology, and a kind of method of steam puffed plant straw fermenting process of preparing hydrogen of clean energy hydrogen is provided.
Technical scheme of the present invention is as follows:
The method of use steam puffed plant straw fermenting process of preparing hydrogen provided by the invention, with the steam-puffed plant straw and stalk is raw material, described steam-puffed plant straw and stalk raw material is that the straw rough lumber is long to 20-30mm, pack in the steam-explosion jar, under 1.5Mpa, dimension was pressed 10 minutes, and instant decompression discharges prepared powdery steam-puffed plant straw and stalk material then; It is characterized in that the hydrogen manufacturing step is:
1) preparation semi-solid ferment substratum:
In the semi-solid ferment reactor, steam-puffed plant straw and stalk raw material, Semen Maydis powder, beef peptone and water evenly are mixed in proportion, make the semi-solid ferment substratum; The weight part proportioning of described steam-puffed plant straw and stalk raw material, Semen Maydis powder, beef peptone and water is the steam-puffed plant straw and stalk raw material: Semen Maydis powder: beef peptone: water=20-40: 0.2-0.8: 0.005-0.02: 100, and the pH value after being mixed is 6.8-7.5;
2) semi-solid ferment that makes is carried out sterilising treatment with substratum;
3) bacterial suspension inoculation:
Will contain in every liter of liquid bacteria suspension 0.5-1 gram acetone-butanol clostridium AS1.135 or/and the liquid bacterial suspension inoculation of Rhodopseudomonas spheroides in above-mentioned semi-solid ferment reactor;
4) fermentative processing makes hydrogen:
At N
2Protection was cultivated 48-72 hour at 35 ℃ of bottom fermentations down, promptly made hydrogen.
The method of use steam puffed plant straw fermenting process of preparing hydrogen provided by the invention also comprises and adopts the gas collection method to collect prepared hydrogen.
Described straw is straw, rice straw, cornstalk or broomcorn straw.
When the bacteria suspension of described access was the mixing liquid bacteria suspension of acetone-butanol clostridium AS1.135 and Rhodopseudomonas spheroides, the volume ratio that both are mixed was: acetone-butanol clostridium AS1.135: Rhodopseudomonas spheroides=1: 1.
The method of use steam puffed plant straw fermenting process of preparing hydrogen provided by the invention, because of its employed raw material is a steam-puffed plant straw and stalk, its aboundresources, cheap, solved the limited deficiency that can not bear the biological hydrogen production main raw material of organic waste water stock number effectively, the factor of created gase that also can overcome in the organic waste water bio-hydrogen production technology is low simultaneously, environment is caused defectives such as secondary pollution.
Embodiment
Further describe the present invention below in conjunction with embodiment.
Embodiment 1, uses quick-fried maize straw of vapour and acetone-butanol clostridium liquid bacteria suspension fermenting process of preparing hydrogen,
Step is as follows:
(1) rough lumber of maize straw raw material is long to 20-30mm, the steam-explosion jar of packing into was pressed 10 minutes in the 1.5Mpa dimension, and instant decompression discharges and makes the quick-fried maize straw raw material of vapour;
(2) in the semi-solid ferment reactor, with the quick-fried maize straw raw material of above-mentioned vapour, Semen Maydis powder, beef peptone and water be by weight ratio: steam puffed stalk raw material: Semen Maydis powder: beef peptone: water=30: 0.5: 0.01: 100 are mixed, pH value after being mixed is 6.8, and the steam sterilizing mode is carried out sterilising treatment routinely again;
(3) will contain the liquid bacterial suspension inoculation of the 1 acetone-butanol clostridium AS1.135 (Clostridium acetobutylicum) that restrains in every liter of liquid bacteria suspension in above-mentioned semi-solid ferment reactor with anaerobism inoculating needle (or syringe);
(4) at N
2Protection was cultivated 48 hours down for 35 ℃ down; Promptly make hydrogen;
(5) collect hydrogen with the gas collection method: at first the hydrogen that produces is fed and contain in the NaOH solution of 0.1N, absorb CO
2, (and drip 3～4 phenolphthalein indicators, work as CO
2When excessive, solution is become colorless by pink), afterwards, hydrogen fed in the gas collection container that saturated aqueous common salt is housed collect hydrogen.
Semi-solid ferment slag to present embodiment carries out drying treatment according to a conventional method, can be directly as the feed that is rich in tropina.
Embodiment 2, use quick-fried straw of vapour and Rhodopseudomonas spheroides liquid bacteria suspension fermenting process of preparing hydrogen, and its step is as follows:
(1) rough lumber of the quick-fried straw raw material of vapour is long to 20-30mm, the steam-explosion jar of packing into was pressed 10 minutes in the 1.5Mpa dimension, and instant decompression discharges and makes the quick-fried straw raw material of vapour;
(2) in the semi-solid ferment reactor, with the quick-fried straw raw material of above-mentioned vapour, Semen Maydis powder, beef peptone and water be by weight ratio: steam puffed stalk raw material: Semen Maydis powder: beef peptone: water=40: 0.2: 0.02: 100 are mixed, pH value after being mixed is 7.0, and the steam sterilizing mode is carried out sterilising treatment routinely again;
(3), every liter of liquid bacteria suspension is contained Rhodopseudomonas spheroides (Rhopseudomonas sphaeroldes) the liquid bacterial suspension inoculation of 0.8 gram in above-mentioned semi-solid ferment reactor with anaerobism inoculating needle (or syringe);
(4) at N
2Protection is cultivated 60h down for 35 ℃ down; Promptly make hydrogen;
(5) collect hydrogen with the gas collection method: at first the hydrogen that produces is fed and contain in the NaOH solution of 0.1N, absorb CO
2, (and drip 3～4 phenolphthalein indicators, work as CO
2When excessive, solution is become colorless by pink), afterwards, hydrogen fed in the gas collection container that saturated aqueous common salt is housed collect hydrogen.
Semi-solid ferment slag to present embodiment carries out drying treatment according to a conventional method, can be directly as the feed that is rich in tropina.
Embodiment 3, quick-fried broomcorn straw of vapour and acetone-butanol clostridium and Rhodopseudomonas spheroides mixing liquid bacteria suspension fermenting process of preparing hydrogen, and its step is as follows:
(1) rough lumber of broomcorn straw raw material is long to 20-30mm, reinstall steam-explosion jar, to press 8--10 minute in the 1.5---1.7Mpa dimension, instant decompression discharges and promptly gets the quick-fried broomcorn straw material of vapour;
(2) in the semi-solid ferment reactor, with the quick-fried broomcorn straw raw material of above-mentioned vapour, Semen Maydis powder, beef peptone and water be by weight ratio: the quick-fried broomcorn straw raw material of vapour: Semen Maydis powder: beef peptone: water=20: 0.8: 0.005: 100 are mixed, pH value after being mixed is 7.5, and the steam sterilizing mode is carried out sterilising treatment routinely again;
(3) with anaerobism inoculating needle (or syringe) every liter of liquid bacteria suspension is contained 0.5 gram Rhodopseudomonas spheroides Rhopseudomonas sphaeroldes) and Rhodopseudomonas spheroides Rhopseudomonassphaeroldes) mixing liquid bacteria suspension (volume ratio of rhodopseudomonas liquid bacteria suspension and Rhodopseudomonas spheroides liquid bacteria suspension is 1: 1), be inoculated in the semi-solid ferment reactor;
(4) at N
2Protection is cultivated 72h down for 35 ℃ down; Promptly make hydrogen;
(5) collect hydrogen with the gas collection method: at first the hydrogen that produces is fed and contain in the NaOH solution of 0.1N, absorb CO
2, (and drip 3～4 phenolphthalein indicators, work as CO
2When excessive, solution is become colorless by pink), afterwards, hydrogen fed in the gas collection container that saturated aqueous common salt is housed collect hydrogen.
Semi-solid ferment slag to present embodiment carries out drying treatment according to a conventional method, can be directly as the feed that is rich in tropina.
Claims (3)
1, a kind of method of using steam puffed plant straw fermenting process of preparing hydrogen, with the steam-puffed plant straw and stalk is raw material, described steam-puffed plant straw and stalk raw material is that the straw rough lumber is long to 20-30mm, pack in the steam-explosion jar, under 1.5Mpa, dimension was pressed 10 minutes, and instant decompression discharges prepared powdery steam-puffed plant straw and stalk material then; It is characterized in that the hydrogen manufacturing step is:
1) preparation semi-solid ferment substratum:
In the semi-solid ferment reactor, steam-puffed plant straw and stalk raw material, Semen Maydis powder, beef peptone and water evenly are mixed in proportion, make the semi-solid ferment substratum; The weight part proportioning of described steam-puffed plant straw and stalk raw material, Semen Maydis powder, beef peptone and water is the steam-puffed plant straw and stalk raw material: Semen Maydis powder: beef peptone: water=20-40: 0.2-0.8: 0.005-0.02: 100, and the pH value after being mixed is 6.8-7.5;
2) semi-solid ferment that makes is carried out sterilising treatment with substratum;
3) bacterial suspension inoculation:
To contain the liquid bacterial suspension inoculation of 0.5-1 gram acetone-butanol clostridium AS1.135 or Rhodopseudomonas spheroides in every liter of liquid bacteria suspension in above-mentioned semi-solid ferment reactor;
4) fermentative processing makes hydrogen:
At N
2Protection was cultivated 48-72 hour at 35 ℃ of bottom fermentations down, promptly made hydrogen.
2, by the method for the described use steam puffed plant straw fermenting process of preparing of claim 1 hydrogen, it is characterized in that, also comprise and adopt the gas collection method to collect prepared hydrogen.
3, by the method for the described use steam puffed plant straw fermenting process of preparing of claim 1 hydrogen, it is characterized in that described straw is straw, rice straw, cornstalk or broomcorn straw.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02148681 CN1254544C (en) | 2002-11-15 | 2002-11-15 | Method for producing hydrogen gas using steam cracked plant fermentation straw |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02148681 CN1254544C (en) | 2002-11-15 | 2002-11-15 | Method for producing hydrogen gas using steam cracked plant fermentation straw |
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| CN1500879A CN1500879A (en) | 2004-06-02 |
| CN1254544C true CN1254544C (en) | 2006-05-03 |
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| CN 02148681 Expired - Fee Related CN1254544C (en) | 2002-11-15 | 2002-11-15 | Method for producing hydrogen gas using steam cracked plant fermentation straw |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008061418A1 (en) * | 2006-11-22 | 2008-05-29 | Institute Of Process Engineering, Chinese Academy Of Sciences | A procedure and equipment thereof for comprehensive utilization of radix pueraria |
| CN100562579C (en) * | 2006-11-07 | 2009-11-25 | 中国科学院过程工程研究所 | Carry out the method for steam explosion stalk fermented product hydrogen by the temperature in the regulation and control fermenting process |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1321187C (en) * | 2004-11-09 | 2007-06-13 | 中国科学技术大学 | Efficient process of preparing hydrogen with botanical waste |
| CN101173303B (en) * | 2006-11-03 | 2012-04-25 | 中国科学院过程工程研究所 | Method for vapor-exploding stalk enzymolysis coupling ferment for hydrogen production by using immobilized cell |
| CN102154371B (en) * | 2011-03-03 | 2013-03-20 | 河南农业大学 | Method for producing hydrogen by straw |
-
2002
- 2002-11-15 CN CN 02148681 patent/CN1254544C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100562579C (en) * | 2006-11-07 | 2009-11-25 | 中国科学院过程工程研究所 | Carry out the method for steam explosion stalk fermented product hydrogen by the temperature in the regulation and control fermenting process |
| WO2008061418A1 (en) * | 2006-11-22 | 2008-05-29 | Institute Of Process Engineering, Chinese Academy Of Sciences | A procedure and equipment thereof for comprehensive utilization of radix pueraria |
| AU2007324208B2 (en) * | 2006-11-22 | 2010-06-10 | Hu Nan Qiangsheng Medicine Co., Ltd. | A procedure and equipment thereof for comprehensive utilization of Radix pueraria |
| US8333999B2 (en) | 2006-11-22 | 2012-12-18 | Institute Of Process Engineering, Chinese Academy Of Sciences | Process for comprehensively utilizing steam exploded radix puerariae and device therefor |
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| Publication number | Publication date |
|---|---|
| CN1500879A (en) | 2004-06-02 |
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Granted publication date: 20060503 Termination date: 20131115 |