CN1247071A - Process for extracting high-content hypericin from Hypericum perforatum - Google Patents
Process for extracting high-content hypericin from Hypericum perforatum Download PDFInfo
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- CN1247071A CN1247071A CN 98114299 CN98114299A CN1247071A CN 1247071 A CN1247071 A CN 1247071A CN 98114299 CN98114299 CN 98114299 CN 98114299 A CN98114299 A CN 98114299A CN 1247071 A CN1247071 A CN 1247071A
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Abstract
A process for extracting high-content hypericin from Hypericum perforation whose hypericia content is 0.01-0.04% includes adding Hypericum perforatum to 0.01-1% solution of NaOH, boiling for 8015 min, filtering to obtain filtrate, adding the dregs to 0.01-1% solution of NaOH, boiling for 8-12 min, filtering to obtain filtrate, merging the filtrate, regulating pH value t 1-5 with hydrochloric acid, eluting with alcohol until it basically has no colour, recovering alcohol, and drying. Its advantages are simplified process, and less consumption of organic solvent.
Description
The present invention relates to the extracting method of effective ingredients in plant, specifically the extracting method of hypericin in the Herba Hyperici perforati.
The hypericin that contains in the Herba Hyperici perforati has superior bioactive, and depression and the nervous a series of neurological disorder diseases such as subhealth symptom that cause are had good elimination effect, paid attention to by international the world of medicine.The international market needs to increase day by day to Herba Hyperici perforati extract, also irritated China in recent years to the production of Herba Hyperici perforati extract.But the hypericin content in the Herba Hyperici perforati has very big-difference (0.01～0.04%) in Different Harvesting Time, the current domestic collection phase to raw material is uncontrollable again, and the extraction process that factory adopts only is suitable for the high-load raw material, can not produce qualified product (content 0.3% is for qualified) to the raw material of low content, caused a large amount of low content raw materials to be wasted, also waste of manpower simultaneously, material resources, and bring bigger loss to enterprise.Therefore select good producing process, the production technology that particularly can be fit to the low content raw material is in demand.
The purpose of this invention is to provide a kind of being suitable for to containing the technology that the low Herba Hyperici perforati of hypericin extracts qualified hypericin, replace original can only be to the technology that high-content hypericin extracts and consumption of organic solvent is big.
Technical essential of the present invention is:
Getting the plant Herba Hyperici perforati, to go into concentration be 0.01～1% NaOH solution, heated and boiled 8～15 minutes, after filtration stand-by filtrate (1); Slag add be filtrate (1) is gone into 1/2～2/3 consumption of same concentration NaOH solution, filtered to get filtrate in 8～12 minutes (2) and filtrate (1) merging through heated and boiled, by the hydrochloric acid adjust pH is 1～5, extremely colourless substantially with ethanol elution then, reclaims the ethanol after drying and gets finished product.Hypericin content in the gained finished product can reach 0.48～0.66%, is higher than criterion of acceptability.
The method that the present invention is given can extract at the Herba Hyperici perforati that contains hypericin 0.01～0.04% and obtain the satisfactory hypericin of content.This method has not only been simplified extraction process, has reduced production cost, and has reduced the consumption of organic solvent.
Embodiment:
1. get Herba Hyperici perforati (containing hypericin 0.01～0.04%) 500g, go into concentration and be 0.01～1% NaOH solution 6000ml, soak and heated and boiled 10 minutes, filter through gauze, gained filtrate (1) is stand-by; Slag adds concentration 0.01～1%NaOH solution 3500ml, boils filtered to get filtrate in 10 minutes (2); It is 1～5 that filtrate merging adds the hydrochloric acid adjust pH, and extremely colourless substantially with ethanol elution then, ethanol elution reclaims the finished product that the ethanol after drying gets hypericin content 0.508%.
2. get the Herba Hyperici perforati 1000g that contains hypericin 0.01～0.04%, it is shaft-like to be cut to 5～10 centimeter length, goes into concentration and be 0.05% NaOH solution 12000ml, heated and boiled 12 minutes, after filtration filtrate (1); It is 0.05% NaOH solution 7000ml that recrement adds concentration, boils filtered to get filtrate in 12 minutes (2); It is 5 that filtrate (1), (2) merging adds the hydrochloric acid adjust pH, then with ethanol elution to colourless substantially, ethanol elution reclaims the ethanol after drying, and to get hypericin content be 0.6% finished product.
Claims (1)
1. method of from Herba Hyperici perforati, extracting high-content hypericin, it is characterized in that getting the plant Herba Hyperici perforati, to go into concentration be 0.01～1% NaOH solution, heated and boiled 8～15 minutes, after filtration stand-by filtrate (1); Slag add be filtrate (1) is gone into 1/2～2/3 consumption of same concentration NaOH solution, filtered to get filtrate in 8～12 minutes (2) and filtrate (1) merging through heated and boiled, by the hydrochloric acid adjust pH is 1～5, extremely colourless substantially with ethanol elution then, reclaims the ethanol after drying and gets finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98114299 CN1247071A (en) | 1998-09-09 | 1998-09-09 | Process for extracting high-content hypericin from Hypericum perforatum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98114299 CN1247071A (en) | 1998-09-09 | 1998-09-09 | Process for extracting high-content hypericin from Hypericum perforatum |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1247071A true CN1247071A (en) | 2000-03-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 98114299 Pending CN1247071A (en) | 1998-09-09 | 1998-09-09 | Process for extracting high-content hypericin from Hypericum perforatum |
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| CN (1) | CN1247071A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100402543C (en) * | 2006-04-30 | 2008-07-16 | 重庆大学 | Preparation method of hyperin and hypericin of Hypericum perforatum |
| CN101744890A (en) * | 2008-12-08 | 2010-06-23 | 上海四埃美微科技有限公司 | Method for refining hypericin in hypericum perfortum extract |
| CN101829164A (en) * | 2010-05-07 | 2010-09-15 | 河南中医学院 | Biological preparation method of Hypericum perforatum L extractive |
| CN104784224A (en) * | 2015-03-25 | 2015-07-22 | 西安天一生物技术股份有限公司 | Preparation method of water-soluble hypericin extract |
| CN105596387A (en) * | 2016-02-05 | 2016-05-25 | 雅安太时生物科技股份有限公司 | Production method of hyperforin perforatum extract |
-
1998
- 1998-09-09 CN CN 98114299 patent/CN1247071A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100402543C (en) * | 2006-04-30 | 2008-07-16 | 重庆大学 | Preparation method of hyperin and hypericin of Hypericum perforatum |
| CN101744890A (en) * | 2008-12-08 | 2010-06-23 | 上海四埃美微科技有限公司 | Method for refining hypericin in hypericum perfortum extract |
| CN101829164A (en) * | 2010-05-07 | 2010-09-15 | 河南中医学院 | Biological preparation method of Hypericum perforatum L extractive |
| CN104784224A (en) * | 2015-03-25 | 2015-07-22 | 西安天一生物技术股份有限公司 | Preparation method of water-soluble hypericin extract |
| CN105596387A (en) * | 2016-02-05 | 2016-05-25 | 雅安太时生物科技股份有限公司 | Production method of hyperforin perforatum extract |
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