CN1238087C - Air filtering net with sterilizing function and preparing method thereof - Google Patents
Air filtering net with sterilizing function and preparing method thereof Download PDFInfo
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- CN1238087C CN1238087C CN 02136366 CN02136366A CN1238087C CN 1238087 C CN1238087 C CN 1238087C CN 02136366 CN02136366 CN 02136366 CN 02136366 A CN02136366 A CN 02136366A CN 1238087 C CN1238087 C CN 1238087C
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- Prior art keywords
- airstrainer
- solution
- antibacterial peptide
- screen pack
- bactericidal action
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Abstract
The present invention relates to an air filter screen with bactericidal action and a preparation method thereof. Two layers are covered on the surface of a filter screen carrier of the filter screen, an inner layer is an adsorption layer, an outer layer is an active layer containing bactericidal activity components, and the bactericidal activity components are antibacterial peptides, or lysostaphins, or chemical antimicrobial agents, or are prepared from two or more than two kinds of active components. The air filter screen with bactericidal action of the present invention has the advantages of obvious bactericidal effect, no special requirements for the filter screen medium, wide application ranges and low cost, and an effective means is provided for people's work and life and air quality improvement.
Description
Technical field
The present invention relates to the sterilization material field, be specifically related to a kind of airstrainer and preparation method with bactericidal action.
Background technology
The screen pack of air-conditioning, air cleaner employing is in long-term use at present, airborne bacterium is adsorbed in the screen pack, because common screen pack is difficult to kill the bacterium that is captured, bacterium breeds on screen pack and causes secondary pollution along with air flows simultaneously.
Chinese patent 97197682.1 discloses a kind of can the air cleaning filter of the direct bactericidal purifying of plankton being fixed with enzyme, dezymotizing protein or peptide and polysaccharide in addition with the covalent bond form on this filter-carrier surface.This invention is had relatively high expectations to carrier, preferably without the borosilicate fiber of water-proofing treatment, have the functional group because of ion-exchange fibre and with the glass fibre of polymer-coated with functional group.
Summary of the invention
Technical problem to be solved by this invention be by on screen pack quantitatively absorption have the active material of kill bacteria effect, make common screen pack have bactericidal action.
Airstrainer with bactericidal action disclosed by the invention is to be coated with two layers at the screen pack carrier surface, and internal layer is an adsorption layer, and is outer for containing the active layer of fungicide active ingredient.
Adsorption layer of the present invention contains CMC-Na and calf serum albumen.
Fungicide active ingredient of the present invention is antibacterial peptide, lysostaphin, chemical antiseptic or above-mentioned two or more assembly of each active component.
Antibacterial peptide of the present invention is an artificial synthetic antimicrobial peptide, be selected from the alpha-helix antibacterial peptide, be rich in cysteine antibacterial peptide, beta sheet antibacterial peptide, contain the antibacterial peptide that abnormal amino acid forms, the antibacterial peptide that contains modified amino acid.
Lysostaphin of the present invention is a kind of metalloproteinases with bacteriolysis through reorganization, dissolves and kills most of Gram-positive pathogenic bacterium, and is especially effective especially to the drug resistance staphylococcus aureus.
Chemical antimicrobial component of the present invention is a chlorhexidine acetate.
Another technical problem to be solved by this invention is the preparation method who discloses above-mentioned screen pack.
The airstrainer that the present invention has bactericidal action makes by following technical proposals:
(1) bSA is dissolved in the CMC-Na solution, is made into the solution that the albumen weight percent concentration is 0.1-5%;
(2) take by weighing the antibacterial activity composition and be dissolved in the Tris-HCl solution of 0.05M pH6.5, be mixed with various concentration on demand;
(3) solution in (1) is evenly sprayed or is coated on the screen pack carrier, or the screen pack carrier is immersed in the adsorbent solution of capacity, drip unnecessary adsorbent again, 70-120 ℃ of oven dry;
(4) will dry the screen pack carrier impregnation of back cooling, evenly spraying or drip (2) solution, or utilize spray-on process, by the requirement of application of sample amount with (2) solution even spraying to screen pack, 35-37 ℃ of oven dry.
A technical problem more to be solved by this invention is to disclose the application of above-mentioned screen pack in air cleaning.
The airstrainer that the present invention has bactericidal action can be used for various air cleaning occasions, as: air-conditioning, refrigerator, air filter etc.
Adopt preparation method of the present invention, can determine the concentration of Adsorption and Sterilization active ingredient on screen pack according to all-environment demand.
Airstrainer of the present invention is carried out relevant BA:
One, quantitatively detects the test of bactericidal effect
(1), template preparation
1, selecting the screen pack medium for use is the plain film of general fibre, and preparation contains the diaphragm of fungicide active ingredient as stated above, and fungicide active ingredient adopts respectively: (1) adopts antibacterial peptide magainin; (2) antibacterial peptide cecropin and lysostaphin; (3) antibacterial peptide defensin and chlorohaxanpyridine acetate.
2, wash fresh test bacterium inclined-plane with 1% peptone PBS, and be diluted to suitable concentration, 10
7～10
8Cfu/ml.
3, sample diaphragm and contrast diaphragm are made 1 * 3cm size, and be placed in the aseptic plate.
4, get 20ul bacterium drop and be added on the diaphragm, smear evenly and pick up counting simultaneously.
5, the diaphragm input is contained in the test tube of 5ml nertralizer with aseptic nipper after affacting the scheduled time, mixing fully vibrates.
6, the contrast diaphragm drops into the nertralizer test tube, fully does suitable dilution after the vibration.
7, get whole liquid 0.5ml and carry out pour plate, 37 ℃ of constant temperature are inverted and were cultivated 48 hours.
8, calculate contrast and reclaim bacterium amount and sample recovery bacterium amount, draw killing rate
9, room temperature was placed 6 months, and killing rate is calculated in repeating step (2)-(8).
(2), result of the test
1. the antibacterial activity composition adopts antibacterial peptide magainin
Table 1
2. the antibacterial activity composition adopts antibacterial peptide cecropin and lysostaphin, adds lysostaphin, and being primarily aimed at drug tolerant bacteria mainly is the drug resistance staphylococcus aureus
Table 1
3. the antibacterial activity composition adopts antibacterial peptide defensin and chlorhexidine acetate, chlorohaxanpyridine acetate (washing must be safe) mixes use with artificial synthetic antimicrobial peptide, help the raising of killing rate, particularly at bacillus, and increase stability of sample, be adapted at comparatively rugged environment.
Table 3
Two, the test of qualitative detection bactericidal effect
(1) test procedure
1, select the plain film of general fibre for use, fungicide active ingredient is selected antibacterial peptide protegrin for use, and the preparation method is with test one.
2, wash fresh inclined-plane with 1% peptone PBS, and be diluted to suitable concentration, about 10
5～10
6Cfu/ml.
3, the nutrient agar that melts is dull and stereotyped, condensation.
4, sample diaphragm and contrast diaphragm are made the disk that diameter is 5mm.
5, the bacterium liquid 200ul that gets after the dilution is added to condensed media surface, and with spreading rod bacterium liquid is evenly smeared out.
6, the flat board that will scribble bacterium liquid is at room temperature placed a moment, makes that bacterium is liquid-solid to be adsorbed on the culture medium surely
7, the disk for preparing is put into planar surface with aseptic nipper, the light pressure makes diaphragm be close to culture medium, between sample and the comparison film certain distance will be arranged, and inhibition zone can fully be expanded
8, the flat board that will post diaphragm is observed after 37 ℃ of constant temperature are inverted 16～18 hours and is had or not inhibition zone to occur, if any the diameter that then measures inhibition zone
(2), experimental result:
To colibacillary antibacterial circle diameter is 13mm, is 12.5mm to the antibacterial circle diameter of staphylococcus aureus, all meets＞the antibacterial requirement of 7mm, and the interpret sample screen pack has good fungistatic effect.Duplicate detection after keeping sample 3 months, antibacterial circle diameter still＞10mm
Above-mentioned result of the test shows, the present invention has the airstrainer of bactericidal action, has significant bactericidal effect, particularly adding lysostaphin kills effective especially to staphylococcus aureus, and can be used for killing of drug resistance golden staphylococci, be particularly suitable for the serious occasion of various air pollutions, as the screen pack of air-conditioning, refrigerator and the circulatory system at hospital, restaurant, station etc.
Airstrainer preparation method of the present invention is simple, and the screen pack medium is not had specific (special) requirements, as cellulose membrane can operate on the net, applied widely, cost is low, is people's work, life, improving air quality provides effective means.
The specific embodiment
Embodiment 1
1.20cm
2The plain film ultraviolet disinfection of ready-made general fibre 30 minutes.
2. get in the CMC-Na solution that the 5mg bSA is dissolved in 1ml 0.5% concentration, making albumin concentration is 0.5%.
3. get the lysostaphin and the 0.5mg antibacterial peptide protegrin of 50 units, be dissolved in the Tris-HCl buffer solution of 5ml 0.05MPH6.5, the concentration that makes lysostaphin is 10u/ml.
4. at 20cm
2Smear on the film or spray and add the mixed solution of 400ul bovine serum albumin(BSA) and CMC-Na, place 80 ℃ of oven dry, guarantee both strong bonded.
5. add the solution 200ul that contains fungicide active ingredient that step 3 makes on cooled film, the final concentration that makes lysostaphin is 0.1u/cm
2, place under the normal temperature and dry, get product.
Embodiment 2
1. the preparation of adsorbent
CMC-Na 0.5g
BSA 0.5g
0.05MpH6.5 Tris-HCl buffer solution 100ml
Compound method is with embodiment 1
2. the preparation of fungicide active ingredient
Lysostaphin 50 units
Chlorhexidine acetate 10mg
Antibacterial peptide magainin 0.5mg
0.05MpH6.5 the Tris-HCl buffer solution
Compound method is with embodiment 1
3. fixation procedure
Get a certain size cellulose membrane, ultraviolet sterilization 30 minutes
The adding adsorbent of smearing or spray, addition is 20ul/cm
2, 75 ℃ of oven dry
The adding fungicide active ingredient of smearing on cooled cellulose membrane or spray, addition reaches 0.1u/cm for making lysostaphin concentration
2, place under the normal temperature and dry, get product.
Claims (4)
1, a kind of airstrainer with bactericidal action is characterized in that being coated with two layers at the screen pack carrier surface, and internal layer is an adsorption layer, and is outer for containing the active layer of fungicide active ingredient; Wherein adsorption layer contains CMC-Na and calf serum albumen; Fungicide active ingredient is antibacterial peptide, lysostaphin, chlorohaxanpyridine acetate or above-mentioned two or more assembly of each active component.
2, a kind of airstrainer with bactericidal action as claimed in claim 1 is characterized in that wherein said antibacterial peptide is an artificial synthetic antimicrobial peptide, is selected from the alpha-helix antibacterial peptide, is rich in the antibacterial peptide and the beta sheet antibacterial peptide of cysteine;
Described lysostaphin is a kind of metalloproteinases with bacteriolysis through reorganization.
3, a kind of preparation method with airstrainer of bactericidal action as claimed in claim 1 is characterized in that this method comprises the following steps:
(1) bSA is dissolved in the CMC-Na solution, is made into the solution that the albumen weight percent concentration is 0.1-5%;
(2) take by weighing the antibacterial activity composition and be dissolved in the Tris-HCl solution of 0.05M, pH6.5, be mixed with various concentration on demand;
(3) solution that step (1) is obtained evenly sprays or is coated on the screen pack carrier, places under 70 ℃ of-120 ℃ of temperature and dries;
(4) will dry the screen pack carrier impregnation of back cooling, evenly spraying or drip the solution that step (2) obtains, after the drying promptly.
4, a kind of application of airstrainer in air cleaning with bactericidal action as claimed in claim 1 is characterized in that this airstrainer is used for the air cleaning occasion, and wherein said air cleaning occasion is meant air-conditioning, refrigerator and air filter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02136366 CN1238087C (en) | 2002-08-02 | 2002-08-02 | Air filtering net with sterilizing function and preparing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02136366 CN1238087C (en) | 2002-08-02 | 2002-08-02 | Air filtering net with sterilizing function and preparing method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1471995A CN1471995A (en) | 2004-02-04 |
| CN1238087C true CN1238087C (en) | 2006-01-25 |
Family
ID=34146431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02136366 Expired - Lifetime CN1238087C (en) | 2002-08-02 | 2002-08-02 | Air filtering net with sterilizing function and preparing method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1238087C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904750A (en) * | 2015-04-21 | 2015-09-16 | 漳州康创净化材料有限公司 | Biological antiseptic, and preparation method and application thereof |
-
2002
- 2002-08-02 CN CN 02136366 patent/CN1238087C/en not_active Expired - Lifetime
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| Publication number | Publication date |
|---|---|
| CN1471995A (en) | 2004-02-04 |
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| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
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| CX01 | Expiry of patent term |
Granted publication date: 20060125 |