CN1218957C - Method for extracting prolamine from corn - Google Patents
Method for extracting prolamine from corn Download PDFInfo
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- CN1218957C CN1218957C CN 03113843 CN03113843A CN1218957C CN 1218957 C CN1218957 C CN 1218957C CN 03113843 CN03113843 CN 03113843 CN 03113843 A CN03113843 A CN 03113843A CN 1218957 C CN1218957 C CN 1218957C
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Abstract
The present invention relates to a producing method for extracting the prolamine from corn. Firstly, ethanol is added in raw materials such as the corn and others, the extraction is carried out through ultrasonic waves, and the centrifugation treatment is carried out; clear liquid is added, and protein is cooled and deposited after the concentration; furthermore, a finished product is obtained through dryness and pulverization. The present invention has the advantage of wide raw material resources, is not limited by production zones, uses ethanol as a single extraction agent, and does not need other organic decolouring agents; the present invention has the advantages of low production cost, high efficiency, high yield rate, etc. The product quality can achieve or approach to a standard of the international same product, raw materials used for production are by-products processed by corn, and the attached value of farm and sideline products can be improved; thereby, the present invention has high social benefit and economic benefit.
Description
(1) technical field
The present invention relates to the zein technical field of producing, specifically be meant a kind of production method of extracting zein.
(2) background technology
Zein is the main storage protein in the corn, because it lacks indispensable amino acids such as Methionin, tryptophane, nutritive value is lower, contain a high proportion of nonpolar amino acid because of it again, be insoluble in water, only be dissolved in 60%～95% aqueous ethanolic solution, thereby in the product processing of food uses, application and development is worth not high.But have flexible, slick, hydrophobic grease proofing corrosion prevention film because zein and polymkeric substance thereof are easy to form, in Biodegradable material flourish today, its this character will have great commercial value.At present, zein has formed focus again in the Application Areas of non-food product.
Domestic also do not have producer can produce zein now, and external existing production technology mainly contains two kinds: a kind of is to be raw material with corn or Zein powder, extract with ethanol, and the hexane decolouring, again with behind the cold water dilution precipitating proteins, dry abrasive dust.This method main drawback be since solvent load big, the running cost of requirement is higher, productive rate low and the extraction the solvent loss height.Another kind is to be raw material with the Zein powder, repeatedly extracts with Virahol, freezing precipitation, drying again, the products obtained therefrom quality is higher, cold operation can be avoided the sex change of zein, yet owing to use a large amount of solvents and low-temperature sludge technology, has obviously increased production cost.
(3) summary of the invention
The present invention is exactly in order to solve above-mentioned the deficiencies in the prior art part, and a kind of production method of extracting zein is provided.This production method production cost is low, efficient is high, and product yield height, quality are good.
A kind of production method of extracting zein of the present invention is characterized in that it comprises the steps and processing condition:
The first step adds 60～95% ethanol stirring in corn or Semen Maydis powder or zein powder raw material, to rise: the liquid material ratio of kilogram counts 4～8: 1, and adjusting pH value scope is 8～13, ultrasonic extraction 10～60 minutes;
Second step was carried out centrifugation with the gained zein, and the centrifugate that obtains is the ethanolic soln of zein;
The 3rd goes on foot centrifugate under-15～-50 ℃, freezing precipitation protein; Or centrifugate below 50 ℃ after the vacuum concentration drying, freezing precipitation protein again;
The 4th step got finished product with the protein drying after the pulverizing.
In order to realize the present invention better, the filter residue in described second step can add an amount of ethanolic soln washing, and is centrifugal again, to improve the yield of zein; According to the requirement of market to different quality zein, described the 3rd step can add cold water below 5 ℃ earlier with the upright precipitation that connects of prolamine, separates; Described the 4th step also can directly add cold water below 5 ℃ with protein precipitation, separate, and then dry, after the pulverizing finished product.
Be applicable to that raw material of the present invention comprises corn, Semen Maydis powder, dry cross or the not dry Zein powder of crossing of Zein powder, partial desiccation.Main extraction agent is ethanol, i.e. alcohol.The kind of raw material, quality and Protein content will produce certain influence to the quality and the yield of finished product.The present invention adopts ultrasonic technology to improve the percentage extraction of zein, shortens the extraction time, because ultrasonic wave has emulsification, homogeneous and heat effect, when extracting, can break the frictional belt effectively, improves extraction rate greatly; After adopting ultrasonic technology, raw material does not need to carry out heat treated again during extraction, and the extraction time shortened to 10～60 minutes by original 4～8 hours, thereby had saved energy, had improved productivity greatly.During the centrifugal treating zein, throw out is gluten and starch etc., can be further used as feed after drying and sell.Since contain a large amount of corn yellow OBs in the centrifugate, the processing of need decolouring, and the present invention adopts the cold method precipitating proteins, also can effectively separate pigment, and can not cause proteinic sex change.
The present invention compared with prior art has following advantage and beneficial effect:
The one, raw material is the by product Zein powder that W-Gum factory produces, and can be dry the mistake, also can be that partial desiccation is crossed, and raw material sources are wide, not limited by the production area; The 2nd, be single extraction agent with ethanol, no longer need other organic discoloring agent, ethanol can reclaim repeatedly, reuses; The 3rd, go out high-quality zein with special explained hereafter.The present invention has that production cost is low, efficient is high, the yield advantages of higher, and quality product reaches or near the standard of international like product.Produce the raw materials used byproduct that is corn processed, improved the attached value of agricultural byproducts, have very high social benefit and economic benefit.Simultaneously, the present invention will fill up the blank that domestic zein is produced.
(4) embodiment
Below in conjunction with embodiment, the present invention is done detailed description further.
The contriver has carried out in depth creative research and test to the present invention, and many successful embodiment are arranged, and enumerates five specific embodiments below.
Embodiment one
The first step is got 125 gram Zein powders, adds 500 milliliters of 70% ethanolic solns, and dispersed with stirring is transferred pH8.0 with sodium carbonate solution, uses probe type ultrasonic wave producer interstitial treatment 60 minutes, and stirs.
Second went on foot in supercentrifuge centrifugation 10 minutes, 400 milliliters of centrifugates.
The 3rd step added 5 ℃ 400 milliliters cold water in centrifugate, get proteins precipitate.
The 4th step was removed supernatant liquor, with the protein drying, got 28 gram zein behind the abrasive dust, and color is yellow partially.
Embodiment two
The first step is got Zein powder 250 grams, adds 1500 milliliter of 80% ethanolic soln, and dispersed with stirring with NaOH solution adjust pH to 12.5, is heated to 50 ℃, handles 25 minutes with the probe type ultrasonic wave producer, and stirs.
Second went on foot in supercentrifuge centrifugation 10 minutes, collected centrifugate.Precipitate washing with alcohol with 200 milliliter 80%, centrifugal again, merge centrifugate.
The 3rd go on foot in-40 ℃ freezing 4 hours, remove supernatant liquid after, throw out.
The 4th step throw out is pulverized through 50 ℃ of dryings, gets zein 67 grams, and product is the cream color, and is glossy.
Embodiment three
The first step is got Zein powder 250 grams, adds 2000 milliliters of 90% ethanol, with sodium carbonate solution adjust pH to 10.0, is heated to 50 ℃, handles 15 minutes with the probe type ultrasonic wave producer, and stirs.
Second went on foot in supercentrifuge centrifugation 10 minutes, centrifugate in 50 ℃ of vacuum concentration to 1/2 of original volume.
The 3rd the step centrifugate in-30 ℃ freezing 8 hours, the precipitation, go supernatant.
The 4th step stirred evenly dispersion, carried out spraying drying, got glassy yellow powdery product 71 grams.
Embodiment four
The first step is got Zein powder 250 grams, adds 1800 milliliters of 75% ethanol, with NaOH solution adjust pH to 12.0, is heated to 50 ℃, handles 40 minutes with the probe type ultrasonic wave producer, and stirs.
Second went on foot in supercentrifuge centrifugation 10 minutes, and precipitation is with 200 milliliter 75% washing with alcohol, and is centrifugal again, merging centrifugate, in 50 ℃ of vacuum concentration to 1/2 of original volume.
The 3rd goes on foot in-18 ℃ of freeze overnight, removes upper strata liquid, gets throw out.
The 4th step throw out is pulverized through 45 ℃ of dryings 24 hours, zein 69 grams.
Embodiment five
The first step is got Zein powder 250 grams, adds 1800 milliliters of 75% ethanol, with NaOH solution adjust pH to 12.0, is heated to 50 ℃, handles 40 minutes with the probe type ultrasonic wave producer, and stirs.
Second went on foot in supercentrifuge centrifugation 10 minutes, and precipitation is with 200 milliliter 75% washing with alcohol, and is centrifugal again, merging centrifugate, in 50 ℃ of vacuum concentration to 1/2 of original volume.
The 3rd goes on foot in-18 ℃ of freeze overnight, removes upper strata liquid, gets throw out.
The 4th goes on foot the cold water that directly adds below 5 ℃ precipitates prolamine matter, separate, and then dry, pulverize, get zein 65 and restrain.
As mentioned above, can realize the present invention preferably.
Claims (4)
1. a production method of extracting zein is characterized in that, it comprises the steps and processing condition:
The first step adds 60～95% ethanol stirring in corn or Semen Maydis powder or zein powder raw material, to rise: the liquid material ratio of kilogram counts 4～8: 1, and adjusting pH value scope is 8～13, ultrasonic extraction 10～60 minutes;
Second step was carried out centrifugation with the gained zein, and the centrifugate that obtains is the ethanolic soln of zein;
The 3rd goes on foot centrifugate under-15～-50 ℃, freezing precipitation protein; Or centrifugate below 50 ℃ after the vacuum concentration drying, freezing precipitation protein again;
The 4th step got finished product with the protein drying after the pulverizing.
2. according to the described a kind of production method of extracting zein of claim 1, it is characterized in that the filter residue in described second step can add an amount of ethanolic soln washing, and is centrifugal again.
3. according to the described a kind of production method of extracting zein of claim 1, it is characterized in that described the 3rd step can add cold water below 5 ℃ earlier with the upright precipitation that connects of prolamine, separates.
4. according to the described a kind of production method of extracting zein of claim 1, it is characterized in that described the 4th step also can directly add cold water below 5 ℃ with protein precipitation, separate, and then dry, after the pulverizing finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03113843 CN1218957C (en) | 2003-03-03 | 2003-03-03 | Method for extracting prolamine from corn |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03113843 CN1218957C (en) | 2003-03-03 | 2003-03-03 | Method for extracting prolamine from corn |
Publications (2)
| Publication Number | Publication Date |
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| CN1435430A CN1435430A (en) | 2003-08-13 |
| CN1218957C true CN1218957C (en) | 2005-09-14 |
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| CN 03113843 Expired - Fee Related CN1218957C (en) | 2003-03-03 | 2003-03-03 | Method for extracting prolamine from corn |
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Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2381760B1 (en) | 2008-12-31 | 2016-12-28 | POET Research, Inc. | Zein composition |
| SG11201401364SA (en) * | 2011-10-11 | 2014-05-29 | Greenstract Llc | Compositions and methods useful for oil extraction |
| EP2969960A4 (en) | 2013-03-15 | 2016-10-19 | Greenstract Llc | Plant-based compositions and uses thereof |
| CN103750242B (en) * | 2013-12-31 | 2015-06-17 | 吉林中粮生化有限公司 | Natural corn condiment and preparation method thereof |
| CN104543327A (en) * | 2014-12-29 | 2015-04-29 | 徐州绿之野生物食品有限公司 | Preparation method of gingko prolamin |
| CN104543333A (en) * | 2014-12-29 | 2015-04-29 | 徐州绿之野生物食品有限公司 | Method for processing alcohol-soluble protein of garlic |
| CN104774253A (en) * | 2015-03-06 | 2015-07-15 | 广东省食品工业研究所 | Preparation method for zein |
| CN106565834B (en) * | 2016-11-07 | 2020-04-07 | 中国科学院兰州化学物理研究所 | Method for decoloring yellow zein |
| CN107550815A (en) * | 2017-09-18 | 2018-01-09 | 上海家化联合股份有限公司 | A kind of maize seed extract and its application in skin preparations for extenal use |
| CN110870499A (en) * | 2019-12-12 | 2020-03-10 | 吉林农业大学 | Plant source fruit and vegetable storage preservative and preparation method thereof |
| CN112209998B (en) * | 2020-10-10 | 2022-06-03 | 国投生物科技投资有限公司 | Ultrasonic-assisted extraction method of corn protein |
| CN114304481A (en) * | 2021-12-15 | 2022-04-12 | 安徽昊晨食品股份有限公司 | Wheat flour rheological property improving process |
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