CN1208739C - Computer controlled low temperature biology microscopic system - Google Patents
Computer controlled low temperature biology microscopic system Download PDFInfo
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- CN1208739C CN1208739C CNB031164862A CN03116486A CN1208739C CN 1208739 C CN1208739 C CN 1208739C CN B031164862 A CNB031164862 A CN B031164862A CN 03116486 A CN03116486 A CN 03116486A CN 1208739 C CN1208739 C CN 1208739C
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- Prior art keywords
- low temperature
- electric expansion
- expansion valve
- computing machine
- liquid nitrogen
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 79
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 39
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 230000003287 optical effect Effects 0.000 claims abstract description 9
- 238000001816 cooling Methods 0.000 claims abstract description 7
- 239000011521 glass Substances 0.000 claims description 22
- 239000006059 cover glass Substances 0.000 claims description 7
- 238000004891 communication Methods 0.000 claims description 5
- 229910001220 stainless steel Inorganic materials 0.000 claims description 4
- 239000010935 stainless steel Substances 0.000 claims description 4
- 230000008676 import Effects 0.000 claims description 3
- 235000013305 food Nutrition 0.000 abstract description 6
- 239000012620 biological material Substances 0.000 abstract description 5
- 238000010438 heat treatment Methods 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 4
- 238000011160 research Methods 0.000 abstract description 2
- 239000006285 cell suspension Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 230000002093 peripheral effect Effects 0.000 abstract 1
- 238000004321 preservation Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 4
- 238000005138 cryopreservation Methods 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000011217 control strategy Methods 0.000 description 1
- 239000002826 coolant Substances 0.000 description 1
- 235000019628 coolness Nutrition 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000008151 electrolyte solution Substances 0.000 description 1
- 229940021013 electrolyte solution Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000011121 hardwood Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229940074869 marquis Drugs 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- VBUNOIXRZNJNAD-UHFFFAOYSA-N ponazuril Chemical compound CC1=CC(N2C(N(C)C(=O)NC2=O)=O)=CC=C1OC1=CC=C(S(=O)(=O)C(F)(F)F)C=C1 VBUNOIXRZNJNAD-UHFFFAOYSA-N 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Images
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- Microscoopes, Condenser (AREA)
Abstract
The present invention discloses a computer controlled low temperature biology microscopic system which is composed of a reverse optical microscope, a low temperature table, a cold and heat supply device and a data acquisition and control system of a computer, etc., wherein a camera is arranged on the reverse optical microscope; a cooled sample (cell suspension liquid or thin layer biological materials) is placed on the low temperature table; cold quantity is supplied by liquid nitrogen, and heating is supplied by a plated film; the temperature of the low temperature table is continuously adjustable in a range of negative 120 to 50 DEG C, the maximal heating speed reaches 100 DEG C / min, and the maximal cooling speed reaches 50 DEG C / min. The computer collects and controls the temperature of the low temperature table and obtains a microscopic image of the refrigerating process in a continuous mode or a timing shoot mode by various plug-in cards and peripheral interfaces. The system performs an important function on the low temperature preservation research field of biology and food materials.
Description
Technical field
The present invention relates to the cryopreservation of biomaterial and food, relate in particular to a kind of computer-controlled low-temperature biological microscopic system.
Background technology
Background of the present invention is the cryopreservation research of biomaterial and food.Cryopreservation field in biomaterial (as blood, sperm, skin, microorganism etc.), cell can be subjected to the damage such as factors such as intracellular ice crystal, high concentration electrolyte solutions in freezing and subsequently rewarming process, make the recovery of biomaterial reduce, thereby be necessary that the variation of pair cell in freezing and rewarming process carry out follow-up study; In the food product refrigeration field, the size of ice crystal and distribution not only influence outward appearance and the taste of food after thawing, and influence the maintenance of food nutrient composition, thereby be necessary the formation and the growth course of ice crystal in the refrigerating process are carried out follow-up study.
Summary of the invention
The purpose of this invention is to provide a kind of computer-controlled low-temperature biological microscopic system.
It has inversion type optical microscope, low temperature platform, cold and hot amount feedway, computer data acquiring and control system; The inversion type optical microscope has condenser, object lens, cold and hot amount feedway has nitrogen cylinder, flowmeter, liquid nitrogen container, coated glass, computer data acquiring and control system have interface circuit, gamma camera, logging, computing machine, first electric expansion valve, second electric expansion valve, the 3rd electric expansion valve, solenoid valve, the signal of gamma camera output enters computing machine by image collection card, temperature on the low temperature platform is measured by micro thermocouple, signal is converted to digital signal through logging, enters computing machine by serial port communication; Computing machine is on the one hand by data acquisition control card control solenoid valve and first electric expansion valve, second electric expansion valve, the switch of the 3rd electric expansion valve and the size of aperture, on the other hand the heat that adds by plated film on the interface circuit control low temperature platform; The required cold of low temperature platform cooling is provided by the liquid nitrogen in the liquid nitrogen container, upper plate, lower plate that the low temperature platform is made by stainless steel are bonded, on upper plate, have the import and export of nitrogen hole, on upper plate, be stained with transparent coated glass, one of coated glass conduction faces down, and also is stained with a glass on lower plate, and sample is placed in the nonconducting one side of coated glass, be covered with cover glass above, temperature thermocouple is bonded to above the cover glass.
Advantage of the present invention: 1) flow control of heat eliminating medium does not need low temperature resistant valve, and the cost of manufacture of system is reduced greatly; 2) add the thermal recovery coated glass, very short from thermal source to the sample room heat transfer distances, reduced temperature controlled hysteresis, improved the stability of temperature control; 3) control of image acquisition and temperature concentrates on the computing machine and finishes, and can obtain corresponding relation visual accurately and temperature, helps further data analysis.
Description of drawings
Fig. 1 is computer-controlled low-temperature biological microscopic system structural representation;
Fig. 2 is computer-controlled low-temperature biological microscopic system low temperature platform structural representation.
Embodiment
Computer-controlled low-temperature biological microscopic system has inversion type optical microscope, low temperature platform, cold and hot amount feedway, computer data acquiring and control system; The inversion type optical microscope has condenser 4, object lens 6, cold and hot amount feedway has nitrogen cylinder 1, flowmeter 2, liquid nitrogen container 3, coated glass 19, computer data acquiring and control system have interface circuit 7, gamma camera 8, logging 9, computing machine 10, first electric expansion valve 11, second electric expansion valve 12, the 3rd electric expansion valve 13, solenoid valve 14, the signal of gamma camera 8 outputs enters computing machine 10 by image collection card, temperature on the low temperature platform 5 is measured by micro thermocouple, signal is converted to digital signal through logging 9, enters computing machine 10 by serial port communication; Computing machine 10 is on the one hand by data acquisition control card control solenoid valve 14 and the switch of electric expansion valve 11,12,13 and the size of aperture, on the other hand the heat that adds by plated film on the interface circuit 7 control low temperature platforms; The required cold of low temperature platform 5 coolings is provided by the liquid nitrogen in the liquid nitrogen container 3.
On the inversion type optical microscope gamma camera is installed, can takes the image under the microscopic field.The low temperature platform is placed on the microscopical objective table, and observed sample then is placed on the low temperature platform.The low temperature platform is bonded by two stainless steel pedestals up and down, be the flow channel of heat eliminating medium between two pedestals, unimpeded for guaranteeing microscopical light path, at the light of two pedestals by all perforates and be stained with glass of position, the glass that wherein is bonded on the top base is coated glass, and the glass that is bonded on the bottom base is simple glass.The plated film face of coated glass is because will conduct electricity, so down, sample just is placed on its nonconducting one side.The cooling of sample is provided by cooling medium flowing between two boards, and the Joule heat that heats up by plated film provides, and its temperature is adjustable continuously in-120～50 ℃ of scopes, and maximum heating rate can reach 100 ℃/minute, and maximum rate of temperature fall can reach 50 ℃/minute.Because the temperature of low temperature platform may if do not take measures, will cause low temperature platform and adjacent domain frosting or dewfall far below environment temperature, thereby light path is hindered or disturbed.For this reason, low temperature platform, microscopical condenser and objection lens portion branch are isolated out, protected with drying nitrogen, the flow of nitrogen is realized by the aperture of regulating first manual modulation valve 15.
The cold of low temperature platform is to be provided by the liquid nitrogen in the liquid nitrogen container, and liquid nitrogen is delivered to the low temperature platform by the insulation pipeline.Liquid nitrogen is constantly evaporation in pipeline, partly or entirely becomes gas phase when arriving the low temperature platform.The mobile power of heat eliminating medium (liquid nitrogen or gas nitrogen) is the high pressure nitrogen in the nitrogen gas container.High pressure nitrogen decompression back is by entering liquid nitrogen container behind electric expansion valve, the flowmeter, and the purpose that electric expansion valve is set is the nitrogen flow that enters liquid nitrogen container in order to control, and the effect of flowmeter is to be convenient to system regulate.Second manual modulation valve 16 is equipped with in pipeline side between liquid nitrogen container and the flowmeter, and its effect is to rush down pressure to liquid nitrogen container when quitting work in system, and this valve is closed when moving in system.Solenoid valve 14 is housed on the pipeline of liquid nitrogen container and low temperature interstation, and its effect is the cooling that opens or closes to the low temperature platform.The 3rd electric expansion valve 13 is housed on the heat eliminating medium outlet conduit of low temperature platform, and its effect is the flow of control by the heat eliminating medium of low temperature platform.
The temperature of sample is measured by micro thermocouple on the low temperature platform, and thermopair is bonded at the upper surface of the cover glass of sample top.Thermocouple signal is introduced into logging (a kind of single-chip data acquisition and Displaying Meter), be converted into digital signal after, enter computing machine with certain communication protocol by serial port communication.Computing machine is determined control strategy except that showing, the storing temperature information, also will recording temperature and design temperature compares according to the deviation situation.At temperature-fall period, be heated to be the master with the control plated film, when the adding heat and surpass a certain upper limit (as 80%) of its total amount of plated film, turn down electric expansion valve, reduce the flow of cool nitrogen, when the adding heat and be lower than a certain lower limit (as 20%) of its total amount of plated film, open big electric expansion valve, increase the flow of cool nitrogen; In the temperature rise period, when heating rate is low, need cool nitrogen, this moment is the same when way is with cooling, promptly go up in limited time above a certain of its total amount, turn down electric expansion valve, reduce the flow of cool nitrogen when the heat that adds of plated film, when a certain following the prescribing a time limit that heat is lower than its total amount that add of plated film, open big electric expansion valve.Owing to be reflected to temperature variation after the fluctuations in discharge hysteresis on the certain hour is arranged, for this reason, after once turning down or open big flow, several acquisition interval such as marquis such as needs grade just can be moved next time.
Computing machine is both by the switch of the solenoid valve on the data acquisition control card control pipeline and the aperture of electric expansion valve, and then control is (directly next from high-pressure nitrogen bottle by the heat eliminating medium or the normal temperature nitrogen of low temperature platform, without liquid nitrogen container) flow, the heat that adds by interface circuit control plated film again.
Image acquisition is to finish by the image collection card on the computing machine, is connected by cable between the gamma camera on image collection card and the microscope.Image acquisition software is attached by image collection card, can carry out continuous acquisition (per second 24 hardwoods), also can be by certain hour interval (as 1 second, 1 minute) timing acquiring.Picture information after the collection is stored with document form, is convenient to further analyzing and processing.
Claims (1)
1. a computer-controlled low-temperature biological microscopic system is characterized in that it has inversion type optical microscope, low temperature platform, cold and hot amount feedway, computer data acquiring and control system; The inversion type optical microscope has condenser (4), object lens (6), cold and hot amount feedway has nitrogen cylinder (1), flowmeter (2), liquid nitrogen container (3), coated glass (19), computer data acquiring and control system have interface circuit (7), gamma camera (8), logging (9), computing machine (10), first electric expansion valve (11), second electric expansion valve (12), the 3rd electric expansion valve (13), solenoid valve (14), the signal of gamma camera (8) output enters computing machine (10) by image collection card, temperature on the low temperature platform (5) is measured by micro thermocouple, signal is converted to digital signal through logging (9), enters computing machine (10) by serial port communication; Computing machine (10) is on the one hand by data acquisition control card control solenoid valve (14) and first electric expansion valve (11), second electric expansion valve (12), the switch of the 3rd electric expansion valve (13) and the size of aperture, controls the heat that adds of plated film on the low temperature platform on the other hand by interface circuit (7); The required cold of low temperature platform (5) cooling is provided by the liquid nitrogen in the liquid nitrogen container (3), upper plate (17), lower plate (18) that low temperature platform (5) is made by stainless steel are bonded, on upper plate (17), have the import and export of nitrogen hole, on upper plate (17), be stained with transparent coated glass (19), one of coated glass (19) conduction faces down, on lower plate, also be stained with a glass (20), sample (22) is placed in the nonconducting one side of coated glass (19), be covered with cover glass (21) above, temperature thermocouple (23) is bonded to above the cover glass (21).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031164862A CN1208739C (en) | 2003-04-16 | 2003-04-16 | Computer controlled low temperature biology microscopic system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031164862A CN1208739C (en) | 2003-04-16 | 2003-04-16 | Computer controlled low temperature biology microscopic system |
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| Publication Number | Publication Date |
|---|---|
| CN1445710A CN1445710A (en) | 2003-10-01 |
| CN1208739C true CN1208739C (en) | 2005-06-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB031164862A Expired - Fee Related CN1208739C (en) | 2003-04-16 | 2003-04-16 | Computer controlled low temperature biology microscopic system |
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| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100346190C (en) * | 2006-03-10 | 2007-10-31 | 浙江大学 | Microscope observation system during freezing dry process |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100461039C (en) * | 2006-09-11 | 2009-02-11 | 中国科学院上海技术物理研究所 | Automatic control system and method for cyclic test in multiple service positions and at high and low temperatures |
| CN102033307B (en) * | 2010-10-15 | 2012-07-04 | 上海理工大学 | Dew prevention device for low-temperature microscope stage |
| CN102183358B (en) * | 2011-01-28 | 2015-01-07 | 复旦大学 | Elliptical polarization instrument sample room device with variable temperature and temperature change method thereof |
| CN102692495B (en) * | 2012-06-14 | 2013-12-11 | 中国科学技术大学 | Perfusion microscope |
| CN107368126A (en) * | 2017-06-26 | 2017-11-21 | 清华大学 | The temperature environment supervising device and its control method of experimental bench |
| CN112683904B (en) * | 2020-12-21 | 2022-04-26 | 中国科学院长春应用化学研究所 | In-situ characterization device and characterization method for interaction between microorganisms and solid surface |
-
2003
- 2003-04-16 CN CNB031164862A patent/CN1208739C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100346190C (en) * | 2006-03-10 | 2007-10-31 | 浙江大学 | Microscope observation system during freezing dry process |
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| Publication number | Publication date |
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| CN1445710A (en) | 2003-10-01 |
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