CN1205436A - Blood analysis instrument dilution and its prepn. method - Google Patents
Blood analysis instrument dilution and its prepn. method Download PDFInfo
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- CN1205436A CN1205436A CN 98101998 CN98101998A CN1205436A CN 1205436 A CN1205436 A CN 1205436A CN 98101998 CN98101998 CN 98101998 CN 98101998 A CN98101998 A CN 98101998A CN 1205436 A CN1205436 A CN 1205436A
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- dilution
- edta
- chlorhexidine acetate
- analysis instrument
- distilled water
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Abstract
A diluent for blood analyzer is prepared from NaCl, KCl, H3BO3, Na2B4O7.10H2O, EDTA-2Na, chlorhexidine acetate and double distilled water through mixing, heating, dissolving, enclosing and filter, and features 282 +/-2 mosm/L of osmotic pressure, 7.6 +/-0.05 of pH value, high anticoagulating and bacteriostatic power.
Description
A kind of analyser reagent of the present invention particularly relates to a kind of blood analysis instrument dilution and preparation method thereof.
The K-4500 blood analyser is the another new product in the K of the company product of Japanese East Asia, is a kind of full-automatic multi parameter analysis instrument, and 18 parameters (three classification), three kinds of cell particle size distribution figures can be provided.Welcome by the demestic user.But its consumables equal dependence on import, particularly dilution, the routine work requirement is big, and import price is very expensive, and application cost is very high.
The blood analysis instrument dilution that the purpose of this invention is to provide a kind of autogamy, it and import reagent there was no significant difference on measurement result is a kind of production domesticization reagent of effect excellence.
Another object of the present invention provides a kind of preparation method of blood analysis instrument dilution of above-mentioned autogamy.
The raw material of blood analysis instrument dilution of the present invention contains Nacl, H
3BO
3, Na
2B
4O
710H
2OEDTA-2Na, Kcl, chlorhexidine acetate and distilled water, the parts by weight proportioning of wherein said various compositions is:
Nacl 65.5-66.5 part Kcl 3.62-3.82 part H
3BO
39.95-10.05 part
Na
2B
4O
710H
2O 1.95-2.05 part EDTA-2Na 3.5-4.5 part
10000 parts of chlorhexidine acetate 0.04-0.06 part distilled water
Wherein, described H
3BO
3, Na
2B
4O
710H
2O not only plays the effect of the PH of buffer solution, but also has the corrosion-resistant function, and collaborative chlorhexidine acetate antiseptic strengthens the bacteriostasis of this reagent.Also the phosphoric acid salt buffer, reduces in the environment because of the superfluous pollution of waterhead that causes of phosphorus nutrition, so preferably use H still in order to reduce the discharging of phosphorus substituting
3BO
3, Na
2B
4O
710H
2O is as the buffer system of solution.
Among the present invention, described EDTA-2Na also can be substituted by EDTA-2K; Described chlorhexidine acetate has antibacterial, and antisepsis also can be substituted by Sodium azide, gentamicin or penicillin; Described distilled water is preferably used distilled water after twice distillation.
All raw material compositions are preferably used the pure level of homemade analysis reagent in the blood analysis instrument dilution of the present invention, with the purity of guaranteeing product and the accuracy of analysis.
The preparation method of blood analysis instrument dilution of the present invention comprises and accurately takes by weighing Nacl, H
3BO
3, Na
2B
4O
710H
2O, EDTA-2Na, Kcl and chlorhexidine acetate, dual distilled water after the Hybrid Heating 50-60 ℃ of dissolving, are that the fabric ester micro-hole filtering film circulating filtration of 0.2 μ m was emitted and got final product in 30-60 minute through enclosed filter device, aperture.
Wherein said enclosed filter device is that 150mm, aperture are that the fabric ester micro-hole filtering film of 0.2 μ m, corresponding stainless-steel tube, valve are formed by stainless steel liquid mixer 100L, medical preparation pump 1100W, the 5 layers of diameter membrane filter that is 150mm and 10 diameters.
Can also be included among this preparation method emit after, the PH that measures reagent is 7.6 ± 0.05, osmotic pressure is 282 ± 2mosm/L, go up machine debugging background multiparameter all meets the requirements, and can use.
By contrast test, blood analysis instrument dilution of the present invention and import reagent are compared:
1, the contrast test of autogamy reagent and import reagent and correlativity: 41 of picked at random inpatients, the 1-2ml that phlebotomizes injects and contains in the import anti-freezing bottle of EDTA-2K, abundant mixing, after placing room temperature (25 ℃ of optimum temperatures) 30min, detect with autogamy reagent and import reagent respectively, want abundant mixing to finish in the 2h after blood sampling before each test, the results are shown in Table 1, unit is legal unit.
2, repeatability is measured: get 1 part of anticoagulation, respectively measure 20 times with autogamy reagent and import reagent respectively, the results are shown in Table the result of 2,15 parameters there are no significant difference, P (2)>0.05.
3, contrast test gained WBC, RBC, three cell particle size distribution figures of PLT overlap the degree height, mostly are fully to overlap.
The contained various chemical constitutions of contrast, repeatability, correlativity, histogram repeatability and dilution by above clinical practice and test findings are all harmless etc. to measuring system, the piping system of instrument, as can be known the complete alternative K-4500 import dilution of autogamy reagent.
Adopting enclosed filter device via hole diameter among the preparation method of blood analysis instrument dilution of the present invention is that the fabric ester micro-hole filtering film of 0.2 μ m was through 30-60 minute circulating filtration, not only remove particulate, but also degerming, reagent background parameter is in full accord with import reagent; Because cellulose esters has any biological inert, has the not characteristic of adsorption charge, can prevent the consumption of ion, so the effect of filtering through its device is better than filtering through G6 glass bacteria filter, more be adapted to produce in batches, and equipment investment is few, production technology is simple, is easy to promote.
The made blood analysis instrument dilution prescription of the present invention advantages of simple, expense is low, and is solution-stabilized, and room temperature can be preserved 4-6 month, can substitute import dilution, economic benefit and obvious social benefit fully.
Further describe the present invention below in conjunction with embodiment and accompanying drawing:
Accompanying drawing 1 is sealing filter process schematic flow sheet;
A kind of blood analysis instrument dilution contains Nacl, H in its raw material
3BO
3, Na
2B
4O
710H
2OEDTA-2Na, Kcl, chlorhexidine acetate and distilled water, the parts by weight proportioning of wherein said various compositions is:
Nacl 66 gram Kcl 3.72 gram H
3BO
310 grams
Na
2B
4O
710H
2O 2 gram EDTA-2Na 4 grams
10 liters of chlorhexidine acetate 0.05 gram distilled water
During preparation, accurately take by weighing earlier analytically pure Nacl, H
3BO
3, Na
2B
4O
710H
2OEDTA-2Na, Kcl and chlorhexidine acetate, dual distilled water, be blended in 60 ℃ of heating for dissolving after, through the enclosed filter device, the aperture is that the fabric ester micro-hole filtering film circulating filtration of 0.2 μ m was emitted and got final product in 60 minutes.And whether measure its PH 7.6 ± 0.05, and whether osmotic pressure is at 282 ± 2mosm/L, and last machine debugging background multiparameter all meets the requirements, and then can use.
The detailed process of described enclosed filter as shown in the figure, dosing is poured into mixer 1, open valve 2 and valve 3, start 4 dosings of pump and get back to mixer 1 through filter 5 along the direction of arrow, go round and begin again, dosing background parameter is qualified until measuring, can twisting activity pipe fitting 6 to 2. position, emit qualified reagent.
Subordinate list 1 autogamy reagent and import reagent contrast test testing result (X ± S)
Reagent type | ??WBC | ???RBC | ???HGB | ????HCT | ???NCV | ???NCH | ????MHC | ???PLT |
Import autogamy t P (2) r | 11.74±5.013 11.75±5.042 0.421 >0.05 0.997 | 3.980±0.764 3.988±0.777 ??0.022 ??>0.05 ??0.886 | 124.2±26.10 124.3±26.07 0.518 >0.05 0.999 | 0.3464±0.0678 0.3465±0.0889 0.300 >0.05 0.991 | 88.10±4.353 88.16±4.357 0.528 >0.05 0.996 | 31.54±2.808 31.64±3.286 0.516 >0.05 0.979 | 357.6±23.03 358.1±30.25 1.662 >0.05 0.970 | 296.0±116.32 298.5±119.14 0.490 >0.05 0.996 |
Continuous table
Reagent type | LYMH# | ???MXD# | ??NEUI# | ??RCW-CV | ??????PIW | ???MPV | ??P-LCR |
Import autogamy t P (2) r | 1.50±0.522 1.50±0.518 ??0.198 ??>0.05 ??0.989 | 0.41±0.308 0.36±0.323 1.785 >0.05 0.825 | 9.84±4.943 9.98±5.085 1.887 >0.05 0.996 | 0.1355±0.02149 0.1359±0.02130 0.725 >0.05 0.987 | 10.65±1.989 10.79±2.181 1.273 >0.05 0.963 | 9.27±1.073 9.29±1.061 0.251 >0.05 0.973 | 0.2054±0.0786 0.2067±0.0805 0.517 >0.05??5 0.979 |
Subordinate list 2 autogamy reagent and import reagent are repeated to be compared (X ± S)
Reagent type | ??WBC | ??RBC | ???HGB | ??HCT | ??NCV | ??NCH | ??MHC | ??PLT |
The import autogamy | 11.13±0.092 10.77±0.103 | 4.007±0.019 4.012±0.020 | 121.1±0.308 120.9±0.353 | 0.3380±0.0024 O.3361±0.0024 | 84.34±0.421 83.77±0.202 | 30.23±0.180 30.18±0.202 | 358.2±2.567 359.7±2.856 | 30.4±6.324 308.9±7.677 |
Continuous table
Reagent type | ??LYMH# | ???MXT# | ??NEUT# | ??RDW-CV | ??PDW | ??MPV | ??P-LCR |
The import autogamy | 2.32±0.082 2.24±0.060 | 0.45±0.151 0.40±0.144 | 836±0.154 8.24±0.146 | 0.1298±0.0024 0.1329±0.0030 | 10.67±0.329 10.93±0.334 | 9.23±0.086 9.31±0.100 | 0.2048±0.0084 0.2123±0.0107 |
Claims (7)
1, a kind of blood analysis instrument dilution is characterized in that raw material contains Nacl, H
3BO
3, Na
2B
4O
710H
2O, EDTA-2Na, Kcl, chlorhexidine acetate and distilled water.
2, dilution as claimed in claim 1 is characterized in that the parts by weight proportioning of wherein said various compositions is:
Nacl 65.5-66.5 part Kcl 3.62-3.82 part H
3BO
39.95-10.05 part
Na
2B
4O
710H
2O 1.95-2.05 part EDTA-2Na 3.5-4.5 part
10000 parts of chlorhexidine acetate 0.04-0.06 part distilled water
3, dilution as claimed in claim 1 is characterized in that described H
3BO
3, Na
2B
4O
710H
2O with phosphate-buffered to substituting.
4, dilution as claimed in claim 1 is characterized in that described EDTA-2Na is substituted by EDTA-2K
5, dilution as claimed in claim 1 is characterized in that described chlorhexidine acetate is substituted by Sodium azide, gentamicin or penicillin.
6, a kind of preparation method of the blood analysis instrument dilution as one of claim 1-5 is characterized in that comprising accurately taking by weighing Nacl, H
3BO
3, Na
2B
4O
710H
2O, EDTA-2Na, Kcl and chlorhexidine acetate, dual distilled water after the Hybrid Heating 50-60 ℃ of dissolving, are that the fabric ester micro-hole filtering film circulating filtration of 0.2 μ m was emitted and got final product in 30-60 minute through enclosed filter device, aperture.
7, preparation method as claimed in claim 6, it is characterized in that also being included in emit after, the PH that measures reagent is 7.6 ± 0.05, osmotic pressure is 282 ± 2mosm/L, go up machine debugging background multiparameter all meets the requirements, and can use.
Priority Applications (1)
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CN 98101998 CN1205436A (en) | 1998-06-01 | 1998-06-01 | Blood analysis instrument dilution and its prepn. method |
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---|---|---|---|
CN 98101998 CN1205436A (en) | 1998-06-01 | 1998-06-01 | Blood analysis instrument dilution and its prepn. method |
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CN1205436A true CN1205436A (en) | 1999-01-20 |
Family
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CN 98101998 Pending CN1205436A (en) | 1998-06-01 | 1998-06-01 | Blood analysis instrument dilution and its prepn. method |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101685050B (en) * | 2008-09-26 | 2012-07-04 | 希森美康株式会社 | Reagent for diluting blood sample and method for measuring mean corpuscular volume by using the same |
CN106525553A (en) * | 2016-11-30 | 2017-03-22 | 刘相东 | Diluent applied to blood analyzer and preparation method thereof |
CN108645597A (en) * | 2018-05-30 | 2018-10-12 | 威海威高血液净化制品有限公司 | dialyzer pressure drop performance test liquid |
CN113176134A (en) * | 2021-04-19 | 2021-07-27 | 广州普道医疗科技有限公司 | Diluent for analyzing blood cells and preparation method thereof |
-
1998
- 1998-06-01 CN CN 98101998 patent/CN1205436A/en active Pending
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101685050B (en) * | 2008-09-26 | 2012-07-04 | 希森美康株式会社 | Reagent for diluting blood sample and method for measuring mean corpuscular volume by using the same |
CN106525553A (en) * | 2016-11-30 | 2017-03-22 | 刘相东 | Diluent applied to blood analyzer and preparation method thereof |
CN108645597A (en) * | 2018-05-30 | 2018-10-12 | 威海威高血液净化制品有限公司 | dialyzer pressure drop performance test liquid |
CN113176134A (en) * | 2021-04-19 | 2021-07-27 | 广州普道医疗科技有限公司 | Diluent for analyzing blood cells and preparation method thereof |
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