CN1189536A - Detoxic agent and prodn. method for microbe and microbe cotton cake - Google Patents
Detoxic agent and prodn. method for microbe and microbe cotton cake Download PDFInfo
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- CN1189536A CN1189536A CN98100824A CN98100824A CN1189536A CN 1189536 A CN1189536 A CN 1189536A CN 98100824 A CN98100824 A CN 98100824A CN 98100824 A CN98100824 A CN 98100824A CN 1189536 A CN1189536 A CN 1189536A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
A detoxicating agent for cottonseed cake is a microbe composition, which is prepared from "Wen No.1" as basis, sacchrifying bacterium and the bacterium AS.1.807. Its detoxicating process is similar to making steamed bread. The conten of gossypol can be reduced from 562 ppm/kg to 140 ppm/kg, lower than national stardard. Detoxicated cottonseed cake can replace 90% or more of beancake used as feed.
Description
The invention belongs to the preparation of microorganism and products thereof.
Culture in China at present and developed into certain scale already.But compare with developed country in the world, the level of pre-capita consumption meat, egg, milk is still lower, thereby aquaculture will still need a great development from now on.The bottleneck of China's development aquaculture is the shortage in feedstuff protein source, the annual mixed fodder of producing of China is more than 3,000 ten thousand tons according to estimates, need more than 600 ten thousand tons in vegetable protein feed, and produce 4,000,000 tons of soybean cake less thaies China's year, formed 2,000,000 tons breach, for these years, many scientific workers are making great efforts to seek to open up new feedstuff protein source always.China is a cotton big country of product, can produce more than 350 ten thousand tons of cottonseed cakes every year, because crude protein content (is equivalent to 80% of soya-bean cake about 36-42% in the cottonseed cake, protein content far above wheat, corn and rice) so numerous scientific and technical personnel just developed it before more than 10 years as potential feed protein source, but progress progress is very slow.The unhappy major cause of making progress is to contain Toxic matter-gossypol in the cottonseed cake, it can seriously damage poultry, the digestion of fowl, reproduction and vision systems, the removing of gossypol is again very difficult, although some scientific and technical personnel finds multiple dephenolize technology technically, but because with high costs and detoxification index shakiness, or other nutrition such as is destroyed at reason, thereby we can say until China in 1996 does not find yet and a kind ofly can accomplish less investment simultaneously, build fast, cost is low, pollution-free, the detoxification index is stable, nutritive ingredient is not destroyed in the cottonseed cake, can also adapt to the detoxification technology of wide farmers.
The objective of the invention is to develop a kind of inexpensive, thing is beautiful, detoxification efficiency is good detoxifying agent, neither need valuable equipment, do not need high temperature, high pressure yet, also without chemical agent or additive, as long as temperature more than 5 ℃ is arranged, no matter on basin, cylinder or concrete floor, the detoxification of all fermenting, its technology is similar to the making steamed bun, 3-5 days detoxification time, the virus elimination rate of per kilogram cottonseed cake is 117.3-140mg, is significantly less than the international standard of 400mg/ kilogram, and crude protein reaches 49.42% (than raising 9.86% before the detoxification) and is made into complete feed and can feeds any poultry, fowl after the detoxification.Through simultaneous test, can replace soya-bean cake 90-94% with the microbial detoxification cottonseed cake, good to the palatability of fowl, poultry, it is all unaffected to culture livestock and poultry.
Content of the present invention is:
A kind of microorganism is named as No. 01, literary composition, and it is a kind of in the thermophilic sporotrichum (sporotrichum thermophile).
The source and the microorganism feature of No. 01 bacterial classification of literary composition:
No. 01 bacterial classification of literary composition is the very strong mould of a kind of saprophytic property, is to use Co
60Gamma-rays 10 Wan Gerui shine " 301 " bacterial classification, form through separation and Culture, name to be ' No. 01, literary composition '.Position in the biology system is a novel bacterial in Mycophyta, deuteromycetes, Moniliales, Moniliaceae, Sporotrichum, the thermophilic sporotrichum strain.No. 01 bacterium colony often is the flannelette blanket shape to literary composition, color is white or light yellow on the PDA substratum, mycelia is slightly shorter than ' 301 ' mycelia, width is 3-10 μ m, a lot of branches are arranged, in a tubular form, every joint is a cell, and mycelia is divided two kinds of dietetic bacterial silk and generative hyphaes, on generative hyphae give birth to sporophore, the many conidiums of adnation come in every shape on stalk.Literary composition can be bred with mycelia for No. 01, also can breed general 24 hours breeding generation with conidium.Literary composition is for No. 01 a kind of middle warm type bacterial classification, and adaptability is very strong, 5 ℃ of minimum requirements, and optimum temperuture is 25-37 ℃, the highest 40-50 ℃, in subzero 10 ℃ and 90 ℃ of also all death of short-term.No. 01 wet fastness of literary composition is strong, requires moisture content in medium at 65-75%, and it is a kind of fungi of good gas, and oxygen requirement is bigger, the normal Co of mycelia
2Content is 0.03%, and vegetative stage is the light requirement photograph not, is suitable for neutrality to alkaline environment in cultivation, and pH value is 6.7-6.9, and optimum carbon nitrogen ratio is 30-40: 1, and can preservation under freezing or exsiccant condition.
The source of amylomyces and biological nature thereof:
Amylomyces and 301 bacterial classification homologies are to use Co in 1994
60Difference choosing system behind gamma-ray irradiation 2441 bacterial classifications, it is the high temperature modification bacterial classification, can secrete saccharifying enzyme, make polysaccharide resolve into monose, its formalness and 301 bacterial classifications are approximate, on nutrient agar, be the flannelette blanket shape, color is a yellow-white, mycelia than 301 bacterial classifications short and a little less than, the production of one, two, three bacterial classification of amylomyces with the literary composition No. 01 identical, after three-class strain is made, after dried and crushed the amylomyces microbial inoculum.
The source of AS.1.807 bacterial classification and biological characteristics thereof:
The AS.1.807 bacterial classification is a kind of bacterium.It can produce proteolytic enzyme, and its bacterial classification prescriptions at different levels all were that Institute of Micro-biology provides from the introducing of Institute of Micro-biology of academy of sciences in 1994.
A kind of microorganism is characterized in that a kind of in the thermophilic sporotrichum (sporotrichum thermophi le), by name ' No. 01 ', literary composition, on February 9th, 1998 was preserved in CGMCC, and preserving number is CGMCC № 0339.
A kind of microorganism cottonseed cake detoxifying agent it is characterized in that based on ' literary composition 01 ' number, add amylomyces and AS.1.807 bacterium the composition composite fungus agent, its prescription is (weight percent):
(1) ' 01 ' number bacterial classification 50-70% of literary composition
(2) amylomyces 15-20%
(3)AS.1.807 15-20％
A kind of manufacture method of microorganism cottonseed cake detoxifying agent comprises the manufacturing of spawn culture and detoxifying agent, it is characterized in that:
(1) ' technological process that 01 ' number bacterial classification of literary composition produced:
(1) first class inoculum is cultivated: first class inoculum expands numerous by the most original inclined-plane first class inoculum, a slant strains can expand and connects 30～40 first class inoculums, and substratum is PDA or wort, and pH value is 6.7～6.9, culture temperature is 38 °-42 ℃, and incubation time is 72 hours.
(2) second class inoculum is cultivated: first class inoculum is inoculated on the wheat substratum, and the making method of wheat substratum is: take by weighing wheat 5Kg earlier, soaked 12 hours in clear water, put into pot again and boil, till wheat does not have the white heart, waterlog out and dry, dry in the air to no open fire in the ventilation, add lime carbonate 15 grams again, gypsum 60 grams, wheat mixed thoroughly get final product, wheat vial packing, pH value are that 6.7～6.9, first class inoculum can connect two bottles, culture temperature is 38～42 ℃, and incubation time is 3～4 days.
(3) three-class strain is cultivated: available second class inoculum expands numerous, and substratum still be a wheat, and general one bottle of secondary kind can expand and connects 30～40 bottles, and three-class strain is drawn out in bottle, dry the back pulverizing three-class strain.
(2) technological process of amylomyces bacterial classification production:
Amylomyces and ' 01 ' number bacterial classification homology of literary composition, the former is the high temperature modification bacterial classification, main secretion saccharifying enzyme, its effect is that polysaccharide is decomposed into monose, and culture temperature is 43～45 ℃, and ' literary composition is for 01 ' number middle warm type bacterial classification, the plain enzyme of its eccrine fiber, its effect is decomposition of cellulose and hemicellulose, and culture temperature is 38～42 ℃, the production process of one, two, three bacterial classification with ' literary composition 01 ' number identical.
(3) technological process of AS.1.807 bacterial classification production:
The AS.1.807 bacterial classification is a kind of bacterium, and it can produce proteolytic enzyme, and its bacterial classification production formulas at different levels are as follows:
(1) first class inoculum substratum: extractum carnis 5 grams, peptone 10 grams, the Nacl0.5 gram, glucose 10 grams, agar 18～20 grams, clear water 1000ml, pH value are 7, culture temperature is 28～32 ℃, incubation time 3～4 days.
(2) second class inoculum substratum: (liquid culture)
Glucose 10 grams, extractum carnis 3 grams, peptone 10 grams, Nacl 0.5 gram, lime carbonate 3-5 gram, clear water 1000ml, pH value are 7.
After making liquid nutrient medium, be sub-packed in the triangular flask of 250-300ml, every bottle of 150ml, sterilization is 1.5 hours under 105 ℃ pressure, after sterilization finishes, wait to be chilled to 25 ℃, dilute first class inoculum with sterilized water, a general first class inoculum connects two bottles, shake every day bottle 3-4 time, culture temperature is 28～32 ℃, cultivates 72 hours, gets final product when a little white hypha appears in liquid level.
(3) three-class strain is cultivated: adopt solid medium, koji tray is cultivated in a large number;
Rice bran 20%, wheat husband 60%, soya-bean cake 10%, Semen Maydis powder 5%, bone meal 5% (weight percent), other adds clear water 25-30%.
In above ratio material is prepared enough, water is mixed material thoroughly, sterilizes 2 hours down at 105 ℃, be sub-packed in while hot in the koji tray, cultivate, when material temperature drop to 25 ℃ at thermostatic chamber, press 10% heavy access liquid spawn of siccative, 28-32 ℃ of cultivation, every day, stirring was 3-4 time, and culture material is topped with sterile cloth or Fibre Bag, cultivated 5 days, when charge level grows white hypha, expect that temperature drop to room temperature gets final product, culture material is taken out oven dry or dries in the shade standby.
The manufacturing of microbial detoxification agent:
Press Semen Maydis powder 30%, wheat husband 30%, cottonseed cake 35%, brown sugar 5% (weight percent) adds former bacterium 5% again, and former bacterium is synthetic by three kinds of bacterium, promptly ' 01 ' number 50-70% of literary composition, amylomyces 15-20%, AS.1.80715-20%, other adds water 50%.
Earlier brown sugar is dissolved in the less water, adds cottonseed cake, Semen Maydis powder and Mai Fu again, then water is filled up, on basin, cylinder or concrete floor, divide three layers of fermentation then, on one, two layer, respectively spread original seed 2%, spread original seed 1% after covering the 3rd layer, pile the steamed bun shape, the top is floating with hand, stamp some ventilation holes, cover gunnysack or cloth is preserved moisture, through fermentation in 3-4 days, white hypha is covered with charge level, and fragrant and sweet flavor is arranged.
Advantage of the present invention:
One, less investment, cost low, with the microbial detoxification agent cottonseed cake is carried out detoxification, both without high temperature, high pressure or chemical agent, more do not need valuable equipment, as long as room temperature keeps more than 5 ℃, the detoxification of can on basin, cylinder and the concrete floor of farm house, all fermenting, it is approximate that steamed bun is made in detoxification process and rural area, and general peasant all can grasp 0.1 yuan of per kilogram cottonseed cake detoxification expense.
Two, detoxification efficiency is good: measure according to agricultural product quality inspection centers (Shandong, Henan) of the Ministry of Agriculture two places, the crude protein of cottonseed cake is 39.62% before the detoxification, gossypol content is 562mg/kg, crude protein rises to 49.48% after the detoxification, gossypol is reduced to 117.3-140mg/kg, gossypol content is than the low 260-282.7mg/kg of national forage standard 400mg/kg, can feed fully each stud bird, poultry; The ratio of crude protein soya-bean cake is high by 6.48%, can replace soya-bean cake 90-94% according to feeding experiment more than a year.Per kilogram soya-bean cake value is 3.3 yuan at present, 1.4 yuan of per kilogram cottonseed cake values, and the per kilogram price difference is 1.9 yuan.Thereby can economize in raw materials cost greatly.
Three, good palatability: after microbial detoxification agent detoxification, be covered with white hypha on the cottonseed cake, i.e. tropina, do not have any peculiar smell, fowl, poultry are all liked to eat, through 5000 kind chickens, 20000 fryer, 13000 laying hens, 15000 meat ducks, more than 4000 of pork pig, 537 of beef cattles, laying hen are laid eggs normally, the growth of meat chicken cycle shortens 5-7 days, and meat duck growth cycle shortens 8-10 days, plants the cock sexual maturity slightly early, plant egg and brood normally, also extremely successful in the raising of pig ox.
Microorganism cottonseed cake detoxifying agent embodiment:
Embodiment one: take by weighing cottonseed cake 100kg earlier, add water 50kg, with 1 kilogram of cottonseed cake detoxifying agent, after fully stirring, pile up at the clean water mire again, on one, two layer, respectively spread 0.4 kilogram of detoxifying agent, spread 0.2 kilogram of detoxifying agent after covering the 3rd layer, floating with hand or plank, stamp some ventilating pits, cover gunnysack or straw screen or mat, through fermentation in 3-5 days, white hypha is covered with charge level, and fragrant and sweet flavor is arranged, through shine dry grinding the detoxification cottonseed cake.
Embodiment two: take by weighing cottonseed cake 98kg earlier, soya-bean cake 2kg adds water 50kg, with 1 kilogram of cottonseed cake detoxifying agent, after fully stirring, in vat, pile up again, on one, two layer, respectively spread 0.4 kilogram of detoxifying agent, spread 0.2 kilogram of detoxifying agent after covering the 3rd layer, floating with hand or plank, stamp some ventilating pits, cover gunnysack or straw screen or mat, through fermentation in 3-5 days, white hypha was covered with charge level, fragrant and sweet flavor is arranged, through shine dry grinding the detoxification cottonseed cake.
Claims (3)
1, a kind of microorganism is characterized in that a kind of in the thermophilic sporotrichum (sporotrichum thermophile), by name ' No. 01 ', literary composition, on February 9th, 1998 was preserved in CGMCC, and preserving number is CGMCC № 0339.
2, a kind of microorganism cottonseed cake detoxifying agent is characterized in that so that ' No. 01 ', literary composition adds the composite fungus agent that amylomyces and AS.1.807 bacterium are formed.
3, a kind of manufacture method of microorganism cottonseed cake detoxifying agent comprises the manufacturing of spawn culture and detoxifying agent, it is characterized in that:
(1) ' technological process that 01 ' number bacterial classification of literary composition produced:
(1) first class inoculum is cultivated: first class inoculum expands numerous by the most original inclined-plane first class inoculum, a slant strains can expand and connects 30～40 first class inoculums, and substratum is PDA or wort, and pH value is 6.7～6.9, culture temperature is 38 °-42 ℃, and incubation time is 72 hours;
(2) second class inoculum is cultivated: first class inoculum is inoculated on the wheat substratum, and the making method of wheat substratum is: take by weighing wheat 5Kg earlier, soaked 12 hours in clear water, put into pot again and boil, till wheat does not have the white heart, waterlog out and dry, dry in the air to no open fire in the ventilation, add lime carbonate 15 grams again, gypsum 60 grams, wheat mixed thoroughly get final product, wheat vial packing, pH value are that 6.7～6.9, first class inoculum can connect two bottles, culture temperature is 38～42 ℃, and incubation time is 3～4 days;
(3) three-class strain is cultivated: available second class inoculum expands numerous, and substratum still be a wheat, and general one bottle of secondary kind can expand and connects 30～40 bottles, and three-class strain is drawn out in bottle, dry the back pulverizing three-class strain;
(2) technological process of amylomyces bacterial classification production:
Amylomyces and ' 01 ' number bacterial classification homology of literary composition, the former is the high temperature modification bacterial classification, main secretion saccharifying enzyme, its effect is that polysaccharide is decomposed into monose, and culture temperature is 43～45 ℃, and ' literary composition is for 01 ' number middle warm type bacterial classification, the plain enzyme of its eccrine fiber, its effect is decomposition of cellulose and hemicellulose, and culture temperature is 38～42 ℃, the production process of one, two, three bacterial classification with ' literary composition 01 ' number identical;
(3) technological process of AS.1.807 bacterial classification production:
The AS.1.807 bacterial classification is a kind of bacterium, and it can produce proteolytic enzyme, and its bacterial classification production formulas at different levels are as follows:
(1) first class inoculum substratum: extractum carnis 5 grams, peptone 10 grams, Nacl 0.5 gram, glucose 10 grams, agar 18～20 grams, clear water 1000ml, pH value are 7, culture temperature is 28～32 ℃, incubation time 3～4 days;
(2) second class inoculum substratum: (liquid culture)
Glucose 10 grams, extractum carnis 3 grams, peptone 10 grams, the Nacl0.5 gram, lime carbonate 3-5 gram, clear water 1000ml, pH value are 7;
After making liquid nutrient medium, be sub-packed in the triangular flask of 250-300ml, every bottle of 150ml, sterilization is 1.5 hours under 105 ℃ pressure, after sterilization finishes, wait to be chilled to 25 ℃, dilute first class inoculum with sterilized water, a general first class inoculum connects two bottles, shake every day bottle 3-4 time, culture temperature is 28～32 ℃, cultivates 72 hours, gets final product when a little white hypha appears in liquid level;
(3) three-class strain is cultivated: adopt solid medium, koji tray is cultivated in a large number;
Rice bran 20%, wheat husband 60%, soya-bean cake 10%, Semen Maydis powder 5%, bone meal 5% (weight percent), other adds clear water 25-30%;
In above ratio material is prepared enough, water is mixed material thoroughly, sterilizes 2 hours down at 105 ℃, be sub-packed in while hot in the koji tray, cultivate, when material temperature drop to 25 ℃ at thermostatic chamber, press 10% heavy access liquid spawn of siccative, 28-32 ℃ of cultivation, every day, stirring was 3-4 time, and culture material is topped with sterile cloth or Fibre Bag, cultivated 5 days, when charge level grows white hypha, expect that temperature drop to room temperature gets final product, culture material is taken out oven dry or dries in the shade standby;
(4) manufacturing of microbial detoxification agent:
Press Semen Maydis powder 30%, wheat husband 30%, cottonseed cake 35%, brown sugar 5% (weight percent) adds former bacterium 5% again, and former bacterium is synthetic by three kinds of bacterium, promptly ' 01 ' number 50-70% of literary composition, amylomyces 15-20%, AS.1.807 15-20%, other adds water 50%;
Earlier brown sugar is dissolved in the less water, adds cottonseed cake, Semen Maydis powder and Mai Fu again, then water is filled up, on basin, cylinder or concrete floor, divide three layers of fermentation then, on one, two layer, respectively spread original seed 2%, spread original seed 1% after covering the 3rd layer, pile the steamed bun shape, the top is floating with hand, stamp some ventilation holes, cover gunnysack or cloth is preserved moisture, through fermentation in 3-4 days, white hypha is covered with charge level, and fragrant and sweet flavor is arranged.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98100824A CN1063225C (en) | 1998-02-18 | 1998-02-18 | Detoxic agent and prodn. method for microbe and microbe cotton cake |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98100824A CN1063225C (en) | 1998-02-18 | 1998-02-18 | Detoxic agent and prodn. method for microbe and microbe cotton cake |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1189536A true CN1189536A (en) | 1998-08-05 |
| CN1063225C CN1063225C (en) | 2001-03-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN98100824A Expired - Fee Related CN1063225C (en) | 1998-02-18 | 1998-02-18 | Detoxic agent and prodn. method for microbe and microbe cotton cake |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946855A (en) * | 2010-09-26 | 2011-01-19 | 湖南农业大学 | Method for detoxifying cotton seed cakes by enzyme method |
| CN102154131A (en) * | 2011-01-11 | 2011-08-17 | 南京农业大学 | Gossypol acetate degrading bacterium S6 |
| CN102154120A (en) * | 2011-01-11 | 2011-08-17 | 南京农业大学 | Gossypol acetate degradation bacteria S1 |
| CN102242118A (en) * | 2010-05-13 | 2011-11-16 | 中国科学院上海生命科学研究院 | Method for improving characters of gossypol in cotton, and use thereof |
| CN104272978A (en) * | 2014-10-30 | 2015-01-14 | 湖南省宇秀生物科技有限公司 | Pleurotus eryngii solid strain liquefaction process |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1078491A (en) * | 1993-02-09 | 1993-11-17 | 中国农业科学院 | A kind of method of quick rotting straws of crops and the microorganism of use thereof |
-
1998
- 1998-02-18 CN CN98100824A patent/CN1063225C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102242118A (en) * | 2010-05-13 | 2011-11-16 | 中国科学院上海生命科学研究院 | Method for improving characters of gossypol in cotton, and use thereof |
| CN102242118B (en) * | 2010-05-13 | 2013-07-10 | 中国科学院上海生命科学研究院 | Method for improving characters of gossypol in cotton, and use thereof |
| CN101946855A (en) * | 2010-09-26 | 2011-01-19 | 湖南农业大学 | Method for detoxifying cotton seed cakes by enzyme method |
| CN101946855B (en) * | 2010-09-26 | 2011-05-18 | 湖南农业大学 | Method for detoxifying cotton seed cakes by enzyme method |
| CN102154131A (en) * | 2011-01-11 | 2011-08-17 | 南京农业大学 | Gossypol acetate degrading bacterium S6 |
| CN102154120A (en) * | 2011-01-11 | 2011-08-17 | 南京农业大学 | Gossypol acetate degradation bacteria S1 |
| CN104272978A (en) * | 2014-10-30 | 2015-01-14 | 湖南省宇秀生物科技有限公司 | Pleurotus eryngii solid strain liquefaction process |
| CN104272978B (en) * | 2014-10-30 | 2016-07-06 | 湖南省宇秀生物科技有限公司 | A kind of Pleurotus eryngii solid spawn liquefaction process |
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| Publication number | Publication date |
|---|---|
| CN1063225C (en) | 2001-03-14 |
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