CN1184891C - Compound pure Chinese medicine agricultural fungicide - Google Patents
Compound pure Chinese medicine agricultural fungicide Download PDFInfo
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- CN1184891C CN1184891C CNB011446714A CN01144671A CN1184891C CN 1184891 C CN1184891 C CN 1184891C CN B011446714 A CNB011446714 A CN B011446714A CN 01144671 A CN01144671 A CN 01144671A CN 1184891 C CN1184891 C CN 1184891C
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Abstract
The present invention discloses a compound pure Chinese medicine agricultural disinfectant prepared from the following components according to the proportion by weight: 3 to 6 portions of lightyellow sophora root, 1 to 3 portions of phellodendron bark, 1 to 3 portions of stemonae, 2 to 6 portions of usnea and 1 to 3 portions of euphorbia helioscopia. The agricultural disinfectant has the advantages of high efficiency, low toxicity, no nuisance, no pollution, no toxic side effect, wide antisepsis ranges and long medicine effect, and has dual functions for preventing and treating various diseases caused by bacteria. Thereby, the agricultural disinfectant can be widely used for preventing and treating the diseases of field crops, fruits, forests and vegetables.
Description
Affiliated field
The present invention relates to the agricultural bactericidal preparation, specifically belong to by the formulated disinfectant use in agriculture of vegetable Chinese herbal medicine.
Background technology
At present, a large amount of chemical pesticides that use carry out preventing and curing diseases of plant on the agricultural.This situation causes serious prestige association to the environment of the mankind and human survival.More alarmingly be to lack very much efficient, low toxicity, novel agrochemical cheaply now on the market.
Summary of the invention
Purpose of the present invention just provides that a kind of bactericidal effect is good, low toxicity, pure Chinese medicine compound prescription disinfectant use in agriculture pollution-free and with low cost.
The object of the present invention is achieved like this, selects following materials of weight proportions for use, i.e. kuh-seng 3-6 part, and golden cypress 1-3 part, tuber of stemona 1-3 part, loose sieve 2-6 part, wartwort 1-3 part is prepared into bactericide.
The preferred weight proportioning of each raw material of the present invention is kuh-seng 3-4 part, golden cypress 1.5-2 part, tuber of stemona 1-1.5 part, loose sieve 2-4 part, wartwort 1.5-2 part.
Concrete preparation method of the present invention is: after above-mentioned each component is pulverized, weight proportion umber by its design mixes, according to percolation, do to leach solvent with the ethanol of 60-90%, flood after 24 hours, with the speed of per minute 1-3ml diacolation slowly, treat that alkaloid oozes out fully, the collection liquid of filtering at concentrating under reduced pressure below 60 ℃, is regulated proportion with concentrate and is got final product to 1.08-1.17.
In use, with concentrate dilute 300-800 doubly after, with the chemical sprayer tool plant is evenly sprayed.
Medicine of the present invention has efficiently, low toxicity, nuisanceless, pollution-free, have no side effect, has a broad antifungal spectrum, the advantage that drug effect is long, the multiple diseases that bacterium is caused has prevention and treatment double action.Can be widely used in the disease control of land for growing field crops crops, fruit-bearing forest vegetables.
Excellent results of the present invention has obtained checking by following experiment.
The present invention prevents and treats the wheat powdery mildew effect experiment:
The experimental group medication select for use the present invention (for ease of the narration, following all the experiment in the present invention all use trade name of the present invention--green precious spirit is explained), for the examination concentration be 800 times, 1000 times, 1500 times.
The control group medication select for use 1 5% triadimefon heading stage mu amount to effective ingredient 6g.The blank group is a clear water.
This test is born by Handan District Ci County and Quzhou County plant protection unit respectively.The sub-district area is made as 1 fen ground (33.33 meters of length, wide 2 meters), each pilot project is all established three repetitions, randomized arrangement, reach the test of 20% o'clock spray medicine in the average sick leaf rate of wheat heading stage, 5 samplings are taked in investigation, every some investigation 20 blades (every sub-district is investigated 100 blades altogether), investigation radix before the dispenser, investigation end in the time of 5,10,15 days respectively after the dispenser.Result of the test shows that 800 times, 1000 times dilutions of green precious spirit are better than triadimefon to the control efficiency of wheat powdery mildew.
The present invention is used to prevent and treat the test of pesticide effectiveness of pear scab:
For studying thing: 10 years living pear trees, kind are pear and snow pear.Growth is normal, and management is general.
Environmental condition: test is selected in theatre, Ma Tun village, Hengshui City Raoyang County.The pear scab morbidity over the years of this garden is serious, and other damage by disease and insect take place lighter, set intercropping peanut in the ranks.The garden thing connects on the limit mutually with pear tree, and north and south and field crop are joined.
Experimental scheme and arrangement:
Medicament: the experiment reagent agent is green precious spirit, and the contrast medicament is gone up 70% mancozeb wettable powder commonly used for producing, and is buied by market.
Application method: spraying.
Spraying equipment: motor atomizer.
Spraying time and number of times: the test region July 4,12 days, 21 days respectively sprayed medicine once on 20th August 5 June 5,20 days May 20, sprayed medicine altogether 8 times, and general control district's control period and number of times are the same.
Formulation rate: average every strain spraying liquid 20Kg, evenly spray, make Ye Guojun soup.
Investigation, record and measuring method:
Meteorology and soil information:
Meteorological data: the daily mean temperature during dispenser: May 20 was 21.5 ℃, June 5 was 28.5 ℃, June 20 was 29.4 ℃, July 4 was 28 ℃, July 12 was 28.1 ℃, July 21 was 28 ℃, August 5 was 24 ℃, August 20 was 28.3 ℃, from the dispenser to the off-test 11 1 days, total rainfall is 669.5mm, and maximum rainfall is 74.9mm, and the heavy rain more than 20 millimeters appears at June 10, June 17, July 14, July 18, July 23, August 6,10 days, 14 days, 16 days, 31 days and September 3,7 days.
Soil information: test theatre soil is clay loam, and pH value is 8, and organic content is medium, irrigated land, but draining in rainy season, annual weeding 4-5 time.
Type of inquiry, time and number of times
Investigation method: adopt and decide the fixed branch of strain investigation method, promptly fix the 34 strain pear trees tree of doing experiment at the pears premorbid, divide 17 sub-districts at random, 2 strains of every sub-district, fix 2 (fruit is arranged) in the different azimuth of every strain tree, look into its branches and leaves fruit, when fruit harvesting same investigation each handle blade and fruit on the fixing branch, put down in writing killed leaf, fruit and do not have scab leaf, the fruit number, obtain disease leaf rate and diseased fruit rate.Sick fruit is also put down in writing the disease of respectively handling on the fruit respectively and counts, and how much is divided into 5 grades by disease point, and the good fruit that does not promptly have scab is a zero level: what 1～5 sick point was arranged is 1 grade, 6～10 sick points are 2 grades, 11～15 be 3 grades, what 16 sick points were above is 4 grades, calculates the disease index that each is handled.
Drug effect computational methods: calculate by following formula:
Sick leaf rate (the %)=total number of sheets of sick number of sheets ÷ * 100%
Diseased fruit rate (the %)=total fruit of disease fruit number ÷ number * 100%
Direct influence to crop: after spraying green precious clever 600 times, the pear tree growth is normal, do not produce any poisoning, and leaf growth is vigorous, and leaf dark green has no adverse effects to other insect pest natural enemies.
Green precious spirit prevents and treats the pear scab result
| The test region | Total number of sheets | The sick number of sheets | Sick leaf rate % | Leaf is prevented effect (%) | The significance of difference (SSR mensuration) | Total fruit number | Sick fruit number | Diseased fruit rate % | Fruit disease feelings index % | The anti-effect (%) of fruit | The significance of difference (SSR mensuration) | ||
| 0.05 | 0.01 | 0.05 | 0.01 | ||||||||||
| 600 times of districts of green precious spirit | 429 | 7 | 1.63 | 98.99 | a | A | 377 | 75 | 19.29 | 6.05 | 80.71 | a | A |
| Contrast chemical control district | 488 | 18 | 3.72 | 96.28 | a | A | 856 | 165 | 19.88 | 6.22 | 80.11 | a | A |
| The blank group | 350 | 223 | 63.71 | 36.29 | d | D | 225 | 149 | 66.22 | 62.58 | 33.78 | c | C |
To find out among this field controling test result: many at rainwater, in the time of the big generation of pear scab, the control efficiency in 600 times of control districts of green precious spirit is best, sick leaf rate only is 1.01%, and diseased fruit rate is 19.89%, and disease index is 6.05%, though diseased fruit rate is high, but the state of an illness is very light, the disease point on the promptly sick fruit seldom, the overwhelming majority is zero level and one-level.This result is better than the general control district (sick leaf rate 3.72%, diseased fruit rate 19.28%, disease index 6.22%) with the conventional dose control.Than the sick leaf rate of blank, diseased fruit rate and disease index reduce by 57.39%, 31.85% and 49.47% respectively.Do not find in the process of the test that fruit tree has any harmful effect.
Use through large tracts of land in the field trials in 2 years, demonstration and the production, green precious spirit has good control efficiency to pear scab, and maximum characteristics are behind fallen flowers and the young fruit period dispenser does not produce any poisoning, and are safe and reliable, have been subjected to numerous orchard workers' favorable comment.
The present invention carries out field experiment to fruit tree, and its result of the test sees the following form:
| Medicament | The control efficiency investigation | ||||||||||
| Kind | Concentration (doubly) | The number of sheets (sheet) | The morbidity number | The incidence of disease (%) | Disease index (%) | Correct cure rate (%) | Fruit number (individual) | The morbidity number | The incidence of disease (%) | Disease index (%) | Correct cure rate (%) |
| Green precious spirit | 600 | 1000 | 10 | 1.0 | 0.5 | 90.90 | 1000 | 5 | 0.5 | 0.3 | 90.38 |
| 800 | 1000 | 15 | 1.5 | 0.9 | 86.36 | 1000 | 8 | 0.8 | 0.7 | 84.61 | |
| Mancozeb | 800 | 1000 | 1.4 | 1.4 | 1.1 | 87.27 | 1000 | 9 | 0.9 | 1.3 | 82.69 |
| — | 1000 | 110 | 11.0 | 19.1 | — | 1000 | 52 | 5.2 | 10.2 | — | |
The present invention is used to prevent and treat the ring rot of apple test of pesticide effectiveness:
Ring spot is the main disease on the apple fruit, and its pathogen is that capsule bacterium subphylum capsule spore shell belongs to Physabosp ora piricola wose, and its asexual generation is the big stem point Pseudomonas wheel big stem point bacterium of line (Macrophoma Kawatsukai Hara).This disease often makes a large amount of fruits rot, and loses economic worth, and loss is serious.Disease was also encroached on limb in the last few years, and especially the Fuji apple morbidity is serious, forms tertia, and the orchard worker claims its " scurf ", and weight person causes dead tree.This sick high-efficiency low-toxicity medicament of control is few at present.For seeking efficient, low toxicity bactericide, we adopt agricultural chemicals of the present invention, and ring rot of apple has been carried out field controling test, so that clear and definite its control efficiency and value thereof.
Test medicine and concentration thereof: 600,800,1000 times of dilutions of green precious medicine liquid, the contrast medicament is 800 times of dilutions of 70% mancozeb wettable powder.
Experimental scheme: the test region is located in the apple district, orchard, Liu Xin Village, Shijiazhuang City suburb, and main breed is state's light, life in 12 years, and 5 * 7 meters of seeding row spacings join between strain, do not have interrow crop in the ranks.It is vigorous to grow, to the full bearing period.5 processing are established in test altogether, and promptly each concentration is a treatment region, every district 15 strains tree, repeat 3 times, 3 sub-districts of promptly every processing, 45 strains tree, 4 are treated to 12 sub-district 180 strains trees, other establishes a blank that does not spray medicine and handles 3 strains tree, establishes 5 altogether and handles seven mu of ground of 183 strains tree, 13 sub-districts.
Test method: the chemical sprayer tool is gone up high voltage power engine-driven atomiser commonly used for producing, even spray medicine, make fruit equal soup, average every strain is with 30 kilograms of left and right sides soups, spray date was was promptly prevented and treated 4 times in August 2,18 days, September 4,22 days altogether in the ring spot generation dip-dye phase.Investigation method adopts the random sampling method, i.e. when the Guoguang apple maturation is gathered (October 17), 1200 left and right sides fruits of random searching in each treatment region are looked into its ring spot real number fruit number of becoming reconciled, and obtain its incidence of disease and cure rate.
Result of the test sees the following form:
Green precious spirit prevents and treats ring spot result of the test table
| Medicament | The control efficiency investigation | |||||
| Kind | Concentration (doubly) | Total fruit number (individual) | Good fruit number (individual) | Sick fruit number (individual) | Diseased fruit rate (%) | Protection effect (%) |
| Green precious spirit | 600 | 1200 | 1152 | 48 | 4.00 | 64.94 |
| Mancozeb | 800 | 1212 | 1162 | 50 | 4.13 | 63.80 |
| 1131 | 1002 | 129 | 11.41 | |||
Above result shows: 600 times green precious spirit is suitable with the control efficiency of 800 times mancozeb, has the efficient sterilizing effect, with the mancozeb difference be that the present invention is the plant type biologic product, therefore nontoxic pollution-free more is better than chemical pesticide.Especially non-harmful gourd, fruit and vegetable more there is using value.
Test shows that the present invention is better than the common chemical medicine to the control efficiency of plant disease in sum, thereby shows that it has good efficacy.
Medicine of the present invention is compared with chemicals also has low toxicity, free of contamination advantage.
The low toxicity of medicine of the present invention, harmless characteristic have obtained checking by following test.
The acute toxicity experience of green precious spirit:
Test method:
According to dosage (Horn ' s) tests and calculates LD50 to incremental method; The breeding observing one of learning from else's experience is all N/R, body weight 180-200 gram healthy SD big white mouse.Contaminating stopped eating in preceding 12 hours can't help water, surveys body weight, is divided into 5 groups then at random, 6 every group.According to pre-test result, once irritate stomach by 1.0ml/100g body weight per os mouth, the check experiment group is irritated clear water, promptly observes poisoning symptom after the administration, and continues to observe a week, record animal dead number.
Result of the test:
The spirit Mi that withers appearred after the administration in 15 minutes, the movable minimizing; Be short of breath subsequently, cry is unusual, and whole body is weak and limp, and is stiff, until last death.Death time concentrated on after the administration in 12 hours.
The animal subject result of the test
| Dosage (mg/kg) | Number of animals (only) | Survival number (only) | Death toll (only) |
| 1,250 2,500 5,000 10000 control groups | 6 6 6 6 6 | 6 6 4 0 6 | 0 0 2 6 0 |
This agent LD50=5612.3mg/kg.
95% fiducial limit: 4190.0-7517.4mg/kg
Conclusion: according to this sampling test result, by " chemistry security toxicological evaluation program " evaluation criterion, this agent per os acute toxicity is a low-toxin farm chemicals.
The skin toxicology test of green precious spirit:
Test method:
According to dosage (Horn ' s) tests and calculates LD50 to incremental method; The breeding observing one of learning from else's experience is all N/R, body weight 180-200 gram healthy SD big white mouse.Contaminate preceding 24 hours back central authorities depilation (4 * 5cm), random packet then, 6 every group.During contamination rat is fixed, each group by 1250,2500,10000mg/kg body weight coating and cover chemical contaminated zone, continue contamination 4 hours, take off covering, wipe residual drug, observe part and whole body poisoning symptom, and record animal dead number.
Result of the test:
After the medication 1.5 hours only the high concentration treated animal lassitude appears, be slow in action movable the minimizing.Recovered normal in 24 hours.
The animal subject result of the test
| Dosage (mg/kg) | Number of animals (only) | Survival number (only) | Death toll (only) |
| 1,250 2,500 5,000 10000 control groups | 6 6 6 6 6 | 6 6 6 6 6 | 0 0 0 0 0 |
This agent LD50＞10000mg/kg
Conclusion: according to this sampling test result, by " chemistry security toxicological evaluation program " evaluation criterion, this agent is a low-toxin farm chemicals through the skin acute toxicity.
The skin irritatin test of green precious spirit
Test method:
1, select 6 of health large ear rabbits for use, body weight 2-3kg tests after experimental situation is raised 24 hours;
2, with every rabbit backbone both sides cropping 3 * 6cm;
3, will examine product 0.5ml behind the 24h, and be applied to every rabbit backbone one side skin of unhairing, then with the gauze covering and with immobilization with adhesive tape, opposite side is done the skin contrast;
4, gently wipe examination while washing with gauze with warm clear water behind the coating 24h, to remove the remaining medicine of skin;
5, observed and recorded remove behind the medicine 1,24,72h and tried erythema in 7,14 days, oedema forms and recovery situation.
Result of the test:
Erythema forms situation: behind the coating 1,24h is tried skin does not have erythema and form, it is no abnormal to contrast a side.
Oedema forms situation: behind the coating 1,24h is tried skin does not have pneumonedema and form.
Skin irritatin is estimated
| Erythema forms integration | Oedema forms integration | Total mark | Skin irritatin strength criterion score value |
| 0 | 0 | 0 | 0 nonirritant |
Conclusion: according to this sampling test result, by " chemistry security toxicological evaluation program " evaluation criterion, this agent is a nonirritant to skin irritatin intensity.
The irritant test to eyes of green precious spirit
Test method:
Select 6 of health large ear rabbits for use, body weight 2-3kg tests after experimental situation is raised 24 hours;
Every rabbit one branch hole eyelid is drop-down gently, get 0.1ml inspection product, splash in the conjunctival sac, closed gently immediately eyelid 1 minute, the branch hole eyeball compares with clear water in addition;
After the administration 1,24,48 and 72h observe cornea, iris, conjunctiva irritant reaction degree, 4,7 days observing response recovery situations;
Eyes vacuum response in the administration 72h, but premature termination test.
Result of the test:
After the medication 1, the congestion of blood vessel of 24h conjunctiva is bright existing, obvious oedema, the companion is secretion in a small amount; The congested swelling of iris; Cornea is normal, and the control sides eyes are normal.
Eye stimulates to be estimated
| Acute eye irritation score index (I.A.O.I) (the highest number) | The average index (M.I.O.I) that eye stimulates | Eye stimulates single index number (I.I.O.I) | Stimulus intensity |
| 13 | Behind the 48h＜5 | —— | Slight excitant |
Conclusion: according to this sampling test result, by " chemistry security toxicological evaluation program " evaluation criterion, this agent is slight excitant to eye irritation intensity.
The sensitization of skin test of green precious spirit:
Test method:
15 of the cavys of select physical health for use, be quick on the draw, appetite is good, body weight 200-250g tests after experimental enviroment is raised 24 hours.
With every guinea pig back center each unhairing 2 * 2cm of both sides, a coating, opposite side compares.
, wipe away gently with clear water behind the 2h in skin of unhairing according to every coating 0.5ml of trial test, the check plot is wiping too, compares observation (Pi Wen, color of the leather, erythema, drying, ulcer or incrustation).Observed 12 continuously, and make detail record.
Rose in 12nd and remake same processing, observed again 12,, can remake test for the third time,, think that then this agricultural chemicals does not cause allergic reaction as allergy not occurring as all there not being change once more.
Result of the test:
Sensitization of skin is estimated
| Sensitization rate (%) | Classification | The intensity classification |
| 7 | 1 | Weak sensitizer |
Conclusion: according to this sampling test result, by pressing " chemistry security toxicological evaluation program " evaluation criterion, this agent is weak sensitizer to sensitization of skin intensity.
The mutagenic test of green precious spirit:
Test method:
Employing infiltration method branch adds and does not add two kinds of S-9 mixed liquors, and dosage is grouped into 10 μ g/ wares; 100 μ g/ wares; 1000 μ mg/ wares and 2000 μ g/ wares, four dosage groups, other establishes blank and positive controls.Positive controls is used 2-AF μ g/ ware.Each dosage group is made three plates, repeats to do twice.
Conclusion: the Ame ' s result of the test of green precious ichor, according to " chemistry security toxicological evaluation program " evaluation criterion, this sample is not found remarkable mutagenesis.
Embodiment
Embodiment 1:
Take by weighing kuh-seng 60kg, golden cypress 20kg, tuber of stemona 20kg, loose sieve 40kg, wartwort 20kg, pulverize respectively and be meal, after the mixing, place percolator, do to leach solvent, with the speed of per minute 2ml diacolation slowly with 70% ethanol, the collection liquid of filtering, below 60 ℃, concentrating under reduced pressure is regulated proportion to 1 with concentrate: about 10.
The preparation method of following examples 2-5 is with embodiment 1, and just the weight proportion of each component is different, but all has effect of the present invention.
Agricultural chemicals of the present invention has the antibacterial effect that is better than chemical bactericide, it can prevent and treat the multiple diseases that is caused by fungi, wheat powdery mildew, pear scab, vegetables downy mildew etc. there is remarkable result, can be widely used in the disease control of land for growing field crops crops, fruit-bearing forest, vegetables, improve output and the quality of crops.Agricultural chemicals of the present invention is the preparation of pure botanical herbs, and raw material sources are extensive, and production technology is simple, so low cost product is suitable for promoting.Agricultural chemicals low toxicity of the present invention, noresidue can not pollute human body and environment in production and use, for the development that promotes the ecological agriculture provides a new good pesticide species.
Claims (2)
1, the pure Chinese medicine disinfectant use in agriculture of a kind of compound is characterized in that it is the bactericide of being made by following materials of weight proportions:
Kuh-seng 3-6 part golden cypress 1-3 part tuber of stemona 1-3 part pine sieve 2-6 part wartwort 1-3 part.
2, the pure Chinese medicine disinfectant use in agriculture of compound according to claim 1 is characterized in that its weight proportion of said raw material is:
Kuh-seng 3-4 part golden cypress 1.5-2 part tuber of stemona 1-1.5 part pine sieve 2-4 part wartwort 1.5-2 part.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011446714A CN1184891C (en) | 2001-12-25 | 2001-12-25 | Compound pure Chinese medicine agricultural fungicide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011446714A CN1184891C (en) | 2001-12-25 | 2001-12-25 | Compound pure Chinese medicine agricultural fungicide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1428083A CN1428083A (en) | 2003-07-09 |
| CN1184891C true CN1184891C (en) | 2005-01-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB011446714A Expired - Fee Related CN1184891C (en) | 2001-12-25 | 2001-12-25 | Compound pure Chinese medicine agricultural fungicide |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822275B (en) * | 2009-10-19 | 2013-05-01 | 成都中医药大学 | Pesticide compound and preparation method and purposes thereof |
| CN104026174A (en) * | 2014-06-26 | 2014-09-10 | 太仓市荣德生物技术研究所 | Sterilizing agent for crops |
| CN105494484A (en) * | 2015-07-10 | 2016-04-20 | 罗友祥 | Technology for preparing crop bactericide by using traditional Chinese medicines |
| CN106490074A (en) * | 2016-11-01 | 2017-03-15 | 南宁马瑞娜装饰工程有限公司 | A kind of antibacterial for landscape tree disease control and preparation method thereof |
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