CN118310981A - Microscopic imaging system based on annular optical coherence detection - Google Patents

Abstract

The invention provides a microscopic imaging system based on annular optical coherence detection, which comprises an OCT module, an inverted microscope, a conical surface reflector and an acquisition processing module, wherein the OCT module comprises a laser source, laser emitted by the laser source is divided into two beams of light with fixed proportion, one beam of light enters a reference arm and then enters a first coupler to serve as reference light of the OCT module, the other beam of light enters a sample arm and then exits, light emitted from the sample arm is reflected to the surface of an imaging object through the inverted microscope and the conical surface reflector to carry out scanning imaging, an imaging object scanning light signal returns to the sample arm and then is received by the first coupler, the two photoelectric detectors are incident after interfering with the reference light, and the acquisition processing module synthesizes panoramic images of the imaging object by utilizing the output of the two photoelectric detectors. The invention combines the advantages of OCT imaging with high resolution and the characteristic of the inverted microscope that the biological sample can be finely imaged.

Description

Microscopic imaging system based on annular optical coherence detection

Technical Field

The invention belongs to the technical field of optical imaging, and particularly relates to a microscopic imaging system based on annular optical coherence detection.

Background

Optical Coherence Refraction Tomography (OCRT) is an advanced imaging technique that combines Optical Coherence Tomography (OCT) and refractive measurement capabilities. OCT is an optical interference imaging technique widely used in clinical research, and can study biological samples included in internal tissues such as embryonic cells from the viewpoint of lateral or depth resolution by using information contained in the amplitude and phase of reflected or scattered light. The principle of biological sample detection is that the light beam of a sample arm is back scattered by tissue to enable tissue cells to generate excitation light signals, the light signals carry tissue structure information to return, a coherent light field is formed together with reflected light received by a reference arm, and the internal structure information of a sample can be obtained by analyzing the coherent light field.

OCT techniques analyze reflected light by interfering with a reference beam as a function of time of wavelength or by dispersing different wavelengths using a grating or other spectral demultiplexer and simultaneously detecting them along a detector array. Generally, conventional OCT has an anisotropic resolution, i.e., the lateral resolution is sacrificed to increase the axial resolution of the imaged object, so that the axial resolution of conventional OCT is generally better than the lateral resolution, and the OCT image is distorted when imaging with refracted light. OCRT by combining measurements of refractive properties of imaged tissue, expands the functionality of OCT and unlike OCT, OCRT can reconstruct higher resolution images with non-anisotropic resolution. Refraction refers to the bending of light rays as they pass from one medium to another (e.g., light rays passing through different layers of the eye). By measuring the refractive index and shape of the tissue, OCRT can provide additional information about the optical properties and structure of the imaged tissue. OCRT involves analysis of the interference patterns of light waves reflected from different tissue layers, by comparing the phases and amplitudes of these light waves, the refractive index and shape of the tissue can be determined. This information can then be used to reconstruct a cross-sectional structural model of the imaged tissue, providing information about its structural and optical properties. Various types of microstructure imaging techniques provide a means of analyzing the surface and subsurface structural characteristics of biological samples. The detection effect on the surface and subsurface of precision components depends on the level of imaging technology, and currently, common methods include: scanning electron microscopy imaging methods and fluorescence imaging methods. However, the method has certain limitations, and the scanning electron microscope detection method has long time and can not directly image the subsurface; the traditional fluorescence imaging method is mainly used for detecting fluorescence emitted by pollution impurities on the surface and subsurface of a product, and can not realize visualization of structural defects.

Disclosure of Invention

The invention aims to solve the defects of the prior OCRT technology and provide a microscopic imaging system based on annular optical coherence detection.

The invention is realized by the following technical scheme:

the microscopic imaging system based on annular optical coherence detection comprises an OCT module, an inverted microscope, a conical surface reflector and an acquisition processing module, wherein the OCT module comprises a laser source, laser emitted by the laser source is divided into two beams of light with fixed proportion, one beam of light enters a reference arm and then enters a first coupler to serve as reference light of the OCT module, the other beam of light enters a sample arm and then exits, light exiting from the sample arm is reflected to the surface of an imaging object through the inverted microscope and the conical surface reflector to carry out scanning imaging, an imaging object scanning light signal returns to the sample arm and is received by the first coupler, the two photoelectric detectors are incident after interfering with the reference light, and the acquisition processing module synthesizes a panoramic image of the imaging object by utilizing the output of the two photoelectric detectors.

Further, the imaging device also comprises a lifting module, wherein the conical reflector and the imaging object are coaxially arranged, the reflecting surface of the conical reflector surrounds the imaging object, and the lifting module is arranged at the bottom end of the imaging object and is used for driving the imaging object to lift along the axis of the conical reflector.

Further, the sample arm comprises a first circulator, light from the laser source is input into the first circulator, and light output by the first circulator is collimated by the first collimator and focused by the scanning lens and then enters the inverted microscope.

Further, the inverted microscope comprises a first plane reflector, light emitted from the scanning lens is input into the first plane reflector, and the light output by the first plane reflector is focused by the tube lens and the objective lens and then is input into the conical surface reflector.

Further, the sample arm further comprises an optical vibrating mirror, and after the light emitted by the first circulator is collimated by the second collimator, the light collimated by the second collimator is turned by the optical vibrating mirror and emitted to the scanning lens.

Further, the device also comprises a driving module, wherein the driving module is connected with the optical galvanometer and is used for adjusting the direction of light output by the optical galvanometer.

Further, the acquisition processing module comprises a workstation and an image reconstruction unit, the workstation is respectively connected with the laser source and the driving module, and the image reconstruction unit synthesizes the panoramic image of the imaging object by utilizing the output of the two photoelectric detectors.

Further, the reference arm includes a second circulator, light from the laser source is input to the second circulator, light output from the second circulator is collimated by the second collimator and focused by the convex lens and then is incident on the second plane mirror, and light returned by reflection of the second plane mirror is incident on the first coupler as reference light.

Further, the OCT module comprises a second coupler, and the laser emitted by the laser source is divided into two beams of light with fixed proportion after passing through the second coupler.

Compared with the prior art, the invention has the beneficial effects that: the OCT module, the inverted microscope and the conical reflector are combined to serve as sources of original detection light, under an imaging mode, the detection light is obtained through combined action of the OCT module, the inverted microscope and the conical reflector, then point focusing is achieved in the conical reflector by the detection light, excitation light signals of an imaging object are further obtained, projection light data under an angle can be obtained after the excitation light signals obtained through sequential complete annular scanning detection are processed by the acquisition processing module, the irradiation direction of light output by the sample arm is controlled, the detection light can be focused on a specific position on the surface of the imaging object, then annular scanning is conducted to obtain the excitation light signals of the complete cross section of the imaging object, and then high-quality panoramic image reconstruction is conducted according to OCRT image reconstruction principles.

Drawings

FIG. 1 is a schematic diagram of a microscopic imaging system based on annular optical coherence detection in accordance with the present invention;

FIG. 2 is an enlarged schematic view of portion A of FIG. 1;

FIG. 3 is a plan view of a conical mirror in a microscopic imaging system based on annular optical coherence detection in accordance with the present invention;

FIG. 4 is a cross-sectional view of a conical mirror in a microscopic imaging system based on annular optical coherence detection in accordance with the present invention;

fig. 5 is a schematic diagram of an imaging procedure of a microscopic imaging system based on annular optical coherence detection according to the present invention.

In the figure, 1-laser source, 2-first coupler, 3-inverted microscope, 31-first plane mirror, 32-tube lens, 33-objective lens, 4-conical mirror, 5-photodetector, 6-acquisition processing module, 61-workstation, 62-image reconstruction unit, 7-lifting module, 8-first circulator, 9-first collimator, 10-scanning lens, 11-optical galvanometer, 12-driving module, 13-second circulator, 14-second collimator, 15-convex lens, 16-second plane mirror, 17-second coupler, 18-imaging object.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures. Meanwhile, in the description of the present invention, the terms "first", "second", and the like are used only to distinguish the description, and are not to be construed as indicating or implying relative importance.

It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.

In the description of the present invention, it should be noted that, directions or positional relationships indicated by terms such as "upper", "lower", "inner", "outer", etc., are directions or positional relationships based on those shown in the drawings, or those that are conventionally put in use, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or elements to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present invention.

Referring to fig. 1 and 2, fig. 1 is a schematic structural diagram of a microscopic imaging system based on annular optical coherence detection according to the present invention, and fig. 2 is an enlarged schematic diagram of a portion a of fig. 1. A microscopic imaging system based on annular optical coherence detection comprises an OCT module, an inverted microscope 3, a conical surface reflector 4 and an acquisition processing module 6, wherein sample detection light of the OCT module is externally connected with the inverted microscope 3 and is reflected to an imaging object 18 through the inverted microscope 3 and the conical surface reflector 4, so that annular scanning of the imaging object 18 is realized, and the acquisition processing module 6 is used for acquiring a panoramic image of the imaging object 18 according to acquired annular scanning data and combining an inversion reconstruction algorithm.

In particular, the OCT module is an interferometric imaging system based on the principles of optical coherence tomography for imaging the imaged object 18. The OCT module comprises a laser source 1, a reference arm, a first coupler 2 and a sample arm, wherein laser emitted by the laser source 1 is divided into two beams of light with fixed proportion, one beam of light enters the reference arm and then enters the first coupler 2 to serve as reference light of the OCT module, the other beam of light enters the sample arm and then exits, the light exiting from the sample arm is reflected to the surface of an imaging object 18 through an inverted microscope 3 and a conical mirror 4 to carry out scanning imaging, an imaging object 18 scans an optical signal and returns to the sample arm and then is received by the first coupler 2, the two beams of light interfere with the reference light and then enter two photodetectors 5, and the acquisition processing module 6 synthesizes a panoramic image of the imaging object 18 by utilizing the output of the two photodetectors 5.

The laser source 1 can be a laser source, and can generate sweep laser with the frequency range of 1280 Hz-1340 Hz and the center frequency of 1310Hz, the laser emitted by the laser source 1 is divided into two beams with fixed proportion and respectively transmitted into the reference arm and the sample arm, in one embodiment, the OCT module comprises a second coupler 17, and the laser emitted by the laser source 1 is divided into two beams with fixed proportion after passing through the second coupler 17. The ratio of the two beams can be determined according to actual requirements, and in this embodiment, the branched beam transmitted to the reference arm has 10% of the optical power of the original laser beam, and the branched beam transmitted to the optical path of the sample arm has 90% of the optical power of the original laser beam. In order to facilitate splitting the laser light emitted from the laser source 1 into two beams of fixed proportion,

In an embodiment, the reference arm includes a second circulator 13, the light from the laser source 1 is input to the second circulator 13, the light output from the second circulator 13 is collimated by the second collimator 14 and focused by the convex lens 15, and then is incident on the second plane mirror 16, and the light reflected back by the second plane mirror 16 is incident on the first coupler 2 as the reference light. In the reference arm, a first output interface of the second circulator 13 is connected to the second collimator 14, and after the second collimator 14 collimates the light emitted from the second circulator 13, a parallel beam of the corresponding branch beam of the laser source 1 is output, along a parallel light path, a convex lens 15 is disposed, the convex lens 15 can focus the parallel beam on a second plane mirror 16, the reflected light of the second plane mirror 16 is reversely transmitted to the second circulator 13 through the convex lens 15 and the collimator, and a second output interface of the second circulator 13 is connected to an input interface of the first coupler 2, so that the reflected light of the second plane mirror 16 is transmitted to the first coupler 2 as reference light.

In one embodiment, the sample arm includes a first circulator 8, light from the laser source 1 is input to the first circulator 8, and light output from the first circulator 8 is collimated by a first collimator 9 and focused by a scanning lens 10 to be incident on the inverted microscope 3. In the sample arm, the first output interface of the first circulator 8 is connected to the first collimator 9, and after the first collimator 9 collimates the light emitted from the first circulator 8, a parallel beam of the corresponding branch beam of the laser source 1 is output, and the parallel beam is focused on a point of a conjugate plane inside the inverted microscope 3 through the scanning lens 10, that is, the scanning lens 10 is placed right in front of the conjugate plane, and the distance between the two is the focal length of the scanning lens 10, so that a complete conjugate beam of the original probe light can be generated. The main function of the conjugate plane is to make the light beam passing through the plane show conjugate symmetry with the light beam before passing through the plane. In order to change the output direction of the light emitted by the sample arm, in an embodiment, the sample arm further includes an optical galvanometer 11, and after the light emitted by the first circulator 8 is collimated by the second collimator 14, the light collimated by the second collimator 14 is turned by the optical galvanometer 11 to be emitted to the scanning lens 10. In an embodiment, the microscopic imaging system based on annular optical coherence detection of the present invention further includes a driving module 12, where the driving module 12 is connected to the optical galvanometer 11, and is used for adjusting the direction of the light output by the optical galvanometer 11. The optical galvanometer 11 has two plane mirrors with different rotation angles inside, and the rotation angles of the two mirrors can be adjusted electrically, and the light beam can be reflected, so that the propagation direction of the laser beam is changed. The driving module 12 is connected to two plane mirrors in the optical galvanometer 11, and is used for adjusting the rotation angles of the two plane mirrors, so as to change the propagation direction of the laser beam output by the optical galvanometer 11. So that the OCT module sample arm output probe light can be focused at a specific location on the surface of the imaging object 18. Further, the two plane mirrors of the optical galvanometer 11 are arranged through an electric rotating bracket, the driving module 12 is connected with the electric rotating bracket, and the rotating angles of the two plane mirrors are respectively adjusted through the corresponding electric rotating bracket.

The inverted microscope 3 is used to conduct the outgoing laser light from the OCT module sample arm, and thus to the cone mirror 4. In one embodiment, the inverted microscope 3 includes a first plane mirror 31, and the light emitted from the scanning lens 10 is inputted to the first plane mirror 31, and the light outputted from the first plane mirror 31 is focused by a tube lens 32 and an objective lens 33 and then is inputted to the conical surface mirror 4. The beam emitted from the sample arm is conjugated at the conjugate plane inside the inverted microscope 3, the conjugate beam is conducted to the first plane mirror 31 to be reflected, the reflected conjugate beam is irradiated to the tube lens 32, the tube lens 32 changes the shape of the conjugate beam into a parallel beam, the parallel beam is conducted to the objective lens 33, and the objective lens 33 focuses the parallel beam and irradiates the inner wall of the conical surface mirror 4.

Referring to fig. 3 and fig. 4 in combination, fig. 3 is a plan view of a conical reflector in a microscopic imaging system based on annular optical coherence detection according to the present invention, and fig. 4 is a sectional view of a conical reflector in a microscopic imaging system based on annular optical coherence detection according to the present invention. The conical reflector 4 is an optical device with a conical reflecting surface for providing a suitable imaging space for the object 18 to be imaged. The conical reflector 4 is adopted, so that the emergent beam of the inverted microscope 3 can be fully reflected. During imaging, the imaging object 18 is placed on the axis of the cone of the conical reflector 4, and after focusing, the light beam is focused at a specific position of the imaging object 18 after being reflected by the inner wall of the conical reflector 4. The probe light enters the imaging object 18 at the focusing point to be refracted, the internal refraction light causes the imaging object 18 to generate excitation light, the excitation light of the imaging object 18 carries the internal structural information of the imaging object 18, and the excitation light of the imaging object 18 is the scanning light signal of the imaging object 18. By adjusting the focal point position of the probe light, circular scanning of the object can be achieved. The excitation light of the imaging object 18 returns to the sample arm after passing through the conical reflector 4 and the inverted microscope 3, so that the energy lost by the excitation light after being conducted in the process is very small, the consistency of the frequency spectrums of the input light and the output light of the light path system can be basically ensured, the imaging depth of the detection light can be ensured to completely penetrate through the internal two-dimensional cross-section structure of the imaging object 18, and the integrity and the accuracy of the acquired structural information are further ensured.

To ensure that the imaging soil surface can be irradiated to a specific position, in one embodiment, the microscopic imaging system based on annular optical coherence detection further comprises a lifting module 7, wherein the conical surface reflector 4 and the imaging object 18 are coaxially arranged, the reflecting surface of the conical surface reflector 4 surrounds the imaging object 18, and the lifting module 7 is arranged at the bottom end of the imaging object 18 and is used for driving the imaging object 18 to lift along the axis of the conical surface reflector 4. The imaging object 18 is driven to lift by the lifting module 7, so that the position of the imaging object 18 in the conical reflector 4 is adjusted, and the position of the imaging object 18 irradiated by the reflected light of the inner wall of the conical reflector 4 is adjusted. The lifting module 7 can adopt the existing lifting equipment such as a lifting table, a telescopic rod and the like. In one embodiment, the lift module 7 and the conical reflector 4 are disposed on the stage of the inverted microscope 3. To further ensure that the reflected light rays of different angles are accurately focused at specific points on the surface of the imaging object 18, in one embodiment, the angle between the reflecting surface of the first planar mirror 31 and the horizontal plane is 45 degrees.

After the excitation light of the imaging object 18 returns to the sample arm, the excitation light is received by the first coupler 2 through the scanning lens 10 and the second collimator 14, and coherent light signals are generated after the excitation light and the reference light interfere with each other, so that corresponding light spectrum information of a two-dimensional structure of the imaging object 18 is obtained. The coherent light signal is then incident on two photodetectors 5, and the two photodetectors 5 acquire phase and amplitude information of the coherent light signal, respectively, and output an electrical signal in which the phase and amplitude information is recorded. After the acquisition processing module 6 receives the optical signals output by the two photodetectors 5, a panoramic image of the imaging object 18 is obtained by combining an inversion reconstruction algorithm. Specifically, the acquisition processing module 6 processes the excitation light signals according to OCRT image reconstruction principles based on the excitation light signals at different angles acquired from one cross section of the imaging object 18, that is, by acquiring the backscattered light of the imaging object 18 in the circular scanning, and reconstructing the acquired signals according to the modeling result of the light propagation path by using a filtered back projection algorithm, so as to obtain a high-quality panoramic image of the two-dimensional cross section structure of the imaging object 18. The acquisition processing module 6 may be an intelligent terminal, such as a computer. In an embodiment, the acquisition processing module 6 comprises a workstation 61 and an image reconstruction unit 62, the workstation 61 being connected to the laser source 1 and the drive module 12, respectively, the image reconstruction unit 62 using the outputs of the two photodetectors 5 to synthesize a panoramic image of the imaged object 18. The laser source 1 and the driving module 12 are controlled by a workstation 61 of the acquisition processing module 6, and an image reconstruction unit 62 is arranged in the acquisition processing module 6 and is used for acquiring a panoramic image of the object based on acquired annular scanning data and combining an inversion reconstruction algorithm.

Referring to fig. 5 in combination, fig. 5 is a schematic diagram illustrating an imaging procedure of a microscopic imaging system based on annular optical coherence detection according to the present invention. The following briefly describes the scanning acquisition process of the microscopic imaging system based on annular optical coherence detection for the imaging object 18 according to the present invention, specifically as follows:

in imaging mode, the workstation 61 emits an impulse response signal to control the laser source 1 of the OCT module to emit laser light, the laser light enters the second coupler 17 from the input interface via the optical fiber, the second coupler 17 splits the laser light into two beams of light as reference light and sample light, respectively, and preferably, the ratio of the reference light to the sample light is set to 1:9. The reference light is then conducted via an optical fiber from the output interface of the second coupler 17 to the input interface of the second circulator 13, the second circulator 13 will conduct the reference light from the first output interface to the second collimator 14, and the second collimator 14 will output a corresponding parallel beam of the reference light. The parallel beam is irradiated to a convex lens 15 having a focal length of 10um and focused on a second plane mirror 16. Along the incident light path of the reference light, the reflected light of the second plane mirror 16 will be back propagated to the second circulator 13 in the form of an optical signal, and then is conducted to the first coupler 2 through the second output interface of the second circulator 13.

At the same time, the sample light output from the second coupler 17 is input from the input interface to the first circulator 8 via the optical fiber, the first circulator 8 transmits the laser light from the first output interface to the first collimator 9, and the first collimator 9 outputs a corresponding parallel beam of the sample light and then reaches the optical galvanometer 11 to change the irradiation direction. The parallel light beam enters the inside of the inverted microscope 3 through the light passing hole of the inverted microscope 3 after being focused by the scanning lens 10, and is focused on one point of the conjugate plane inside the inverted microscope 3, and then conducted and focused on the light path of the inverted microscope 3 through the plane mirror, the tube lens 32 and the objective lens 33, so that excitation light is formed. The excitation light is reflected on the inner wall of the conical reflector 4. The reflected beam is focused on a specific point on the surface of the object 18 to be imaged, thereby performing scanning probe imaging. The imaging object 18 is placed on a lifting module 7, and the lifting module 7 can move up and down to adjust the position and the height of the imaging object 18, so as to change the corresponding cross section of the focusing point on the surface of the imaging object 18, and then obtain the structural information of different cross sections of the object. The main principle of probe imaging is that excitation light enters the interior of the imaged object 18 to be refracted, and the object is caused to generate an excitation light signal, which contains information about the cross-sectional structure of the object. After the excitation light signal is generated, the excitation light signal is reversely propagated to the first circulator 8 according to the conduction path of the sample light, and is conducted to the first coupler 2 through the second output interface of the first circulator 8.

The optical signals from the reference arm and the sample arm interfere with each other after being input to the first coupler 2, and a coherent optical signal is generated, and then the two photodetectors 5 acquire phase and amplitude information of the coherent optical signal, respectively. After the phase and amplitude information of the coherent light signals is acquired, the two photodetectors 5 generate electrical signals that are input to the image reconstruction unit 62 of the acquisition processing module 6.

In addition, when the propagation direction of the original sample light needs to be changed, the workstation 61 of the acquisition processing module 6 can send out a signal to control the driving module 12, and the driving module 12 can electrically adjust the rotation angles of the two plane mirrors in the optical galvanometer 11, so that the propagation direction of the excitation light can be changed from different angles, and the surrounding adjustment of the focus point position of the reflected sample light beam on the surface of the imaging object 18 can be realized, and the surrounding scanning of the imaging object 18 can be formed, so that the ring imaging data can be obtained. In addition, since it is necessary that the sample light be conducted to be focused on a specific point of the imaging object 18, the focal length of the objective lens 33 is adjusted according to the actual propagation of the probe beam.

The image reconstruction unit 62 processes the output electric signals of the two photodetectors 5, and can obtain a high-quality panoramic image of the two-dimensional cross-sectional structure of the corresponding imaging object 18. Specifically, according to the OCRT image reconstruction principle described in Kevin c.zhou, the high quality panoramic image reconstruction procedure is as follows:

First we have to perform refraction correction on the acquired annular imaging data. For one of the cross-sectional structures of the imaged object 18 at one projection angle, when we do not know its ray propagation trajectory matrix, we can solve the inverse problem using the ray equation as a forward model, in 2D (x and y):

Where n _A (x, z) is an estimate of the refractive index profile matrix with a as a parameter, s is the position along the 1D ray trajectory, a is the initial object cross-section refractive index profile matrix, of size 128 x 128, which is initially predictively assigned by the imaging space parameters (dielectric refractive index, height and aperture of the cone mirror 4).

Then, under different projection angles, we solve the obtained ray propagation trajectory graph according to the forward model, perform refraction correction on the annular imaging data with corresponding angles, and reconstruct a high-quality panoramic image of the cross section of the imaging object 18 by combining the corrected imaging data with different angles through a filtered back projection algorithm. After the panoramic image is obtained, the image is subjected to inverse transformation of filtering back projection, so that predictive annular imaging data under different angles are generated, the maximum mean square error calculation is carried out between the data and original annular imaging data under corresponding angles, and then the calculation result is added with the refractive index distribution matrix, so that the cyclic iterative updating of the refractive index distribution matrix, the light propagation track diagram, the high-quality panoramic image and the predictive annular imaging data is realized.

The present invention is not limited to the preferred embodiments, and any simple modification, equivalent variation and modification made to the above embodiments according to the technical substance of the present invention will still fall within the scope of the technical solution of the present invention.

Claims (9)

1. The microscopic imaging system based on annular optical coherence detection is characterized by comprising an OCT module, an inverted microscope, a conical surface reflector and an acquisition processing module, wherein the OCT module comprises a laser source, laser emitted by the laser source is divided into two beams of light with fixed proportion, one beam of light enters a reference arm and then enters a first coupler to serve as reference light of the OCT module, the other beam of light enters a sample arm and then exits, light exiting from the sample arm is reflected to the surface of an imaging object through the inverted microscope and the conical surface reflector to carry out scanning imaging, an imaging object scanning light signal returns to the sample arm and then is received by the first coupler, the imaging object scanning light signal interferes with the reference light and then enters two photoelectric detectors, and the acquisition processing module synthesizes panoramic images of the imaging object by utilizing the output of the two photoelectric detectors.

2. The microscopic imaging system based on annular optical coherence detection according to claim 1, further comprising a lifting module, wherein the conical surface reflector is coaxially arranged with the imaging object, the reflecting surface of the conical surface reflector surrounds the imaging object, and the lifting module is arranged at the bottom end of the imaging object and is used for driving the imaging object to lift along the axis of the conical surface reflector.

3. The microscopic imaging system of claim 1, wherein the sample arm includes a first circulator, light from the laser source is input to the first circulator, and light output from the first circulator is collimated by the first collimator and focused by the scanning lens and then is incident on the inverted microscope.

4. The microscopic imaging system based on annular optical coherence detection according to claim 3, wherein the inverted microscope comprises a first plane mirror, light emitted from the scanning lens is input into the first plane mirror, and light output by the first plane mirror is focused by the tube lens and the objective lens and then is input into the conical surface mirror.

5. The microscopic imaging system according to claim 3, wherein the sample arm further comprises an optical galvanometer, and the light emitted by the first circulator is collimated by the second collimator, and the light collimated by the second collimator is diverted by the optical galvanometer to be emitted to the scanning lens.

6. The microscopic imaging system based on annular optical coherence detection of claim 5, further comprising a driving module connected to the optical galvanometer for adjusting the direction of the light output by the optical galvanometer.

7. The microscopic imaging system based on annular optical coherence detection according to claim 6, wherein the acquisition processing module comprises a workstation and an image reconstruction unit, the workstation being connected to the laser source and the driving module, respectively, the image reconstruction unit synthesizing a panoramic image of the imaged object using the outputs of the two photodetectors.

8. The microscopic imaging system based on annular optical coherence detection of claim 1, wherein the reference arm includes a second circulator, light from the laser source is input to the second circulator, light output from the second circulator is collimated by a second collimator and focused by a convex lens and then is incident on a second plane mirror, and light reflected back by the second plane mirror is incident on the first coupler as the reference light.

9. The microscopic imaging system based on annular optical coherence detection according to claim 1, wherein the OCT module includes a second coupler, and the laser light emitted from the laser source is split into two beams of light with a fixed ratio after passing through the second coupler.