CN118202944A - Breeding method for generating X-ray radiation through electron accelerator - Google Patents
Breeding method for generating X-ray radiation through electron accelerator Download PDFInfo
- Publication number
- CN118202944A CN118202944A CN202410541853.XA CN202410541853A CN118202944A CN 118202944 A CN118202944 A CN 118202944A CN 202410541853 A CN202410541853 A CN 202410541853A CN 118202944 A CN118202944 A CN 118202944A
- Authority
- CN
- China
- Prior art keywords
- breeding
- radiation
- seeds
- ray radiation
- treatment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000005855 radiation Effects 0.000 title claims abstract description 97
- 238000009395 breeding Methods 0.000 title claims abstract description 66
- 230000001488 breeding effect Effects 0.000 claims abstract description 56
- 238000000034 method Methods 0.000 claims abstract description 34
- 230000035772 mutation Effects 0.000 claims abstract description 18
- 230000009946 DNA mutation Effects 0.000 claims abstract description 7
- 208000035240 Disease Resistance Diseases 0.000 claims abstract description 6
- 238000012216 screening Methods 0.000 claims description 23
- 230000012010 growth Effects 0.000 claims description 22
- 238000011156 evaluation Methods 0.000 claims description 16
- 238000002360 preparation method Methods 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 5
- 230000007613 environmental effect Effects 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 239000002609 medium Substances 0.000 claims description 3
- 238000010899 nucleation Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 35
- 230000008569 process Effects 0.000 abstract description 14
- 230000006872 improvement Effects 0.000 abstract description 12
- 238000009396 hybridization Methods 0.000 abstract description 6
- 238000003976 plant breeding Methods 0.000 abstract description 6
- 230000001965 increasing effect Effects 0.000 abstract description 5
- 230000001939 inductive effect Effects 0.000 abstract description 4
- 238000010362 genome editing Methods 0.000 abstract description 3
- 231100000350 mutagenesis Toxicity 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 description 6
- 238000012271 agricultural production Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 230000007614 genetic variation Effects 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000003889 chemical engineering Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005272 metallurgy Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to the technical field of bioengineering, and discloses a breeding method for generating X-ray radiation through an electron accelerator, which accelerates the breeding process: x-ray radiation breeding utilizes electron accelerators to generate X-ray radiation, which can induce mutation of plants in a relatively short time, thereby accelerating breeding process and creating plant varieties: by inducing DNA mutation, new plant varieties can be created, and the varieties possibly have better disease resistance, adaptability or other required characteristics, thereby enriching plant breeding resources and improving mutation rate: the X-ray radiation can induce wide DNA mutation, compared with the traditional natural hybridization or artificial hybridization, the mutation rate can be improved, the diversity and the selectivity of breeding are increased, and the directional improvement is realized: specific target plant seeds can be selected for radiation treatment, and specific gene editing or improvement can be carried out on known varieties, so that the directional improvement of plant characteristics is realized.
Description
Technical Field
The invention relates to the technical field of bioengineering, in particular to a breeding method for generating X-ray radiation through an electron accelerator.
Background
Bioengineering is a combination of various subjects such as molecular genetics, microbiology, cell biology, biochemistry, chemical engineering and energy science, and has a very wide application range, including aspects of medicine, food, agriculture and forestry, gardening, chemical industry, metallurgy, oil extraction, new technology of fermentation tanks, environmental protection of new substrates and the like. Many existing microbiologically based industries are improved by means of genetic engineering and utilization, and social problems such as environmental pollution are relieved. In the near future, photobioreactors and biofuels will become realized, and structurally complex but renewable substrates like lignocellulose will become raw materials for the fermentation industry, and will probably also provide starting components for the plastics industry and the polymer industry. It can be said that genetic engineering and cellular engineering are the basis of biological engineering, recombinant DNA technology and enzyme immobilization technology are two of the most distinctive and potential technologies of biological engineering, while fermentation engineering and cell and tissue culture technology are relatively mature and widely used technologies.
The following are the problems that the X-ray radiation breeding scheme aims to solve:
1. Time consumption: conventional plant breeding processes require a significant amount of time to develop new varieties with desirable characteristics. 2. Genetic variation rate limitation: traditional breeding methods are limited by lower genetic variation rate, and are difficult to obtain diversity. 3. Variety resource is deficient: the traditional breeding process may face the problem of lack of variety resources, limiting the selectivity and diversity of breeding. 4. Characteristic orientation improvement is difficult: the conventional breeding method is difficult to realize the directional improvement of specific target characteristics. 5. The success rate is low: in the traditional breeding process, the success rate of breeding is lower due to low genetic variation rate and limited screening method. Through the X-ray radiation breeding scheme, the problems can be effectively solved, and the efficiency and the success rate of plant breeding are improved.
Disclosure of Invention
(One) solving the technical problems
Aiming at the defects of the prior art, the invention provides a breeding method for generating X-ray radiation through an electron accelerator, which solves the technical problems in the prior art.
(II) technical scheme
In order to achieve the above purpose, the present invention provides the following technical solutions: a breeding method for generating X-ray radiation by means of an electron accelerator, comprising the steps of:
s1, preparing;
S2, selecting target plant seeds;
s3, treating seeds;
S4, setting accelerator parameters;
s5, radiation treatment;
S6, radiation post-treatment;
S7, observing and screening;
S8, subsequent reproduction and evaluation.
Preferably, the preparation: the required equipment and materials are prepared, including electron accelerators, plant seeds, culture medium, growth chambers.
Preferably, the target plant seed is selected: selecting seeds of a target plant to be bred.
Preferably, the seeds are treated: the selected seeds are subjected to a preliminary treatment.
Preferably, the accelerator parameters are set: the parameters of the electron accelerator are adjusted according to the required radiation dose and energy.
Preferably, the radiation treatment: the prepared seeds are placed in the radiation area of the accelerator, the accelerator is started to carry out radiation treatment, and the seeds are subjected to X-ray radiation, so that DNA mutation occurs.
Preferably, the radiation post-treatment: the irradiated seeds are removed from the irradiated areas and subjected to subsequent treatment. This may include seeding on the medium, providing appropriate nutrients and environmental conditions.
Preferably, observation and screening: the growth of the treated seeds was monitored to see if the desired mutation profile was present. If desired, screening can be performed and plants with the desired characteristics can be further cultivated.
Preferably, subsequent propagation and evaluation: subsequent breeding and evaluation work is performed on the selected mutants, for example, to observe their growth traits, physiological characteristics, disease resistance, etc., to determine whether they meet breeding goals, and to further develop stable new varieties.
(III) beneficial effects
Compared with the prior art, the beneficial effect of this scheme is as follows:
1. accelerating the breeding process: x-ray radiation breeding utilizes electron accelerators to generate X-ray radiation, which can induce mutation of plants in a relatively short time, thereby accelerating breeding progress.
2. Creating a plant variety: by inducing mutations in the DNA, new plant varieties may be created that may have better disease resistance, adaptability, or other desirable characteristics, thereby enriching plant breeding resources.
3. The mutation rate is improved: the X-ray radiation can induce wide DNA mutation, compared with the traditional natural hybridization or artificial hybridization, the mutation rate can be improved, and the diversity and the selectivity of breeding are increased.
4. Orientation improvement: specific target plant seeds can be selected for radiation treatment, and specific gene editing or improvement can be carried out on known varieties, so that the directional improvement of plant characteristics is realized.
5. Improving the success rate of breeding: by observing and screening the stage, further cultivation can be performed for plants with the desired characteristics, thereby increasing the probability of success of breeding.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Embodiment one:
A breeding method for generating X-ray radiation by means of an electron accelerator, comprising the steps of: s1, preparing; s2, selecting target plant seeds; s3, treating seeds; s4, setting accelerator parameters; s5, radiation treatment; s6, radiation post-treatment; s7, observing and screening; s8, subsequent reproduction and evaluation, and preparation: preparing required equipment and materials including electron accelerator, plant seeds, culture medium and growth chamber, selecting target plant seeds: selecting target plant seeds to be bred, and treating the seeds: preliminary treatment is carried out on the selected seeds, and accelerator parameters are set: adjusting parameters of the electron accelerator according to the required radiation dose and energy, and carrying out radiation treatment: placing the prepared seeds in the radiation area of the accelerator, starting the accelerator to perform radiation treatment, wherein the seeds are subjected to X-ray radiation, so that DNA is mutated, and performing radiation post-treatment: the irradiated seeds are removed from the irradiated areas and subjected to subsequent treatment. This may include seeding on the medium, providing appropriate nutrients and environmental conditions, observing and screening: the growth of the treated seeds was monitored to see if the desired mutation profile was present. Screening can be performed, plants with desired characteristics can be further cultivated, subsequently propagated and evaluated, as desired: subsequent breeding and evaluation work is performed on the selected mutants, for example, to observe their growth traits, physiological characteristics, disease resistance, etc., to determine whether they meet breeding goals, and to further develop stable new varieties.
The invention has the following beneficial effects that the breeding process is accelerated: x-ray radiation breeding utilizes electron accelerators to generate X-ray radiation, which can induce mutation of plants in a relatively short time, thereby accelerating breeding process and creating plant varieties: by inducing DNA mutation, new plant varieties can be created, and the varieties possibly have better disease resistance, adaptability or other required characteristics, thereby enriching plant breeding resources and improving mutation rate: the X-ray radiation can induce wide DNA mutation, compared with the traditional natural hybridization or artificial hybridization, the mutation rate can be improved, the diversity and the selectivity of breeding are increased, and the directional improvement is realized: specific target plant seeds can be selected for radiation treatment, and specific gene editing or improvement can be carried out on known varieties, so that the directional improvement of plant characteristics is realized, and the breeding success rate is improved: by observing and screening the stage, further cultivation can be performed for plants with the desired characteristics, thereby increasing the probability of success of breeding.
Method for operating X-ray radiation breeding scheme
X-ray radiation breeding is a method of inducing plant mutation by X-ray radiation to accelerate the breeding process and create new plant varieties. The operation flow of this scheme, including the steps of pre-preparation, radiation treatment, post-management, etc., will be described in detail below.
The specific working procedure of the invention is as follows:
1. Early preparation
Before starting X-ray radiation breeding, a series of preliminary preparations are required:
selecting a target variety: selecting target plant varieties which need to be subjected to breeding improvement, and ensuring that the target plant varieties have a certain genetic background and germplasm advantages.
Preparing a breeding facility: ensuring proper breeding facilities including planting boxes, culture media, growing lamps, etc. to provide a proper growing environment.
Screening seeds: and screening and treating the seeds of the selected target variety to ensure the quality and purity of the seeds.
Determining the radiation dose: depending on the nature of the target plant and the desired degree of mutation, a suitable X-ray radiation dose is determined.
2. Radiation treatment
Radiation treatment is a critical step in X-ray radiation breeding, requiring strict control of radiation dose and treatment time to ensure a balance of mutation rate and plant growth.
Setting radiation conditions: the operating parameters of the radiation device, including the radiation time, the radiation distance, etc., are set according to the radiation dose determined in advance.
Seed treatment: the prepared seeds are placed in a suitable irradiation vessel to ensure that the seeds are uniformly irradiated.
Radiation treatment: the irradiation equipment is started to carry out irradiation treatment on the seeds, and the irradiation time and the irradiation dose are controlled so as to achieve the expected mutation effect.
3. Post-management
After the radiation treatment is completed, post-management is needed to promote the growth and development of mutant seeds and the screening and cultivation of new varieties.
Sowing seeds: the seeds after the radiation treatment are sown in a culture medium or soil to provide proper moisture and nutrients.
And (3) observing the growth condition: the growth conditions of the mutant seeds, including growth rate, leaf morphology, etc., are observed periodically, and the mutant manifestation is recorded.
Screening mutants: and screening mutants with target characteristics according to the expected mutation effect, and further culturing and breeding.
Variety identification: and (3) carrying out variety identification and evaluation on the screened mutants to ensure that the mutants meet breeding targets and market demands.
4. Promotion of new varieties
After a series of screening and cultivation, the new variety with good characteristics is determined, and the method can be popularized and applied.
Variety popularization: the new variety is promoted to agricultural production practice, trial planting and demonstration promotion are carried out, and more farmers and growers can know and adopt the new variety.
Market application: the new variety is introduced to the market, the requirements of consumers are met, and the development and upgrading of the agricultural industry are promoted.
Through the operation flow, the X-ray radiation breeding scheme can be effectively implemented, the breeding process is accelerated, new varieties are created, the breeding efficiency and success rate are improved, and more high-quality plant resources are provided for agricultural production.
Wherein the implementation step of the invention comprises the following sub-steps:
S1, preparing work
1. Selecting a target plant variety: the target plant variety with breeding potential is carefully selected, and factors such as adaptability, yield, quality and the like are considered.
2. Preparing a breeding facility: ensure the cleaning and disinfection of breeding facilities, provide proper illumination, temperature and humidity conditions, and provide good environment for the growth of seeds.
3. Preparing a seed material: the selected seeds are treated and disinfected, so that the selected seeds are guaranteed to be disease-free, insect-free and good in quality, and the purity and stability of the irradiated mutants are improved.
S2, selecting target plant seeds
1. Determining seed source: reliable seed suppliers or partners are selected, and the source traceability and the quality of seeds are ensured.
2. Checking seed quality: each batch of seeds was checked for appearance and quality, eliminating damaged, deformed or contaminated seeds.
3. Seed pretreatment: the selected seeds are pre-treated, e.g., soaked, sterilized, etc., to enhance their sensitivity to radiation.
S3, seed treatment
1. Soaking treatment: the seeds are soaked in a proper amount of soaking liquid for a period of time to increase the water absorption and sensitivity of the seeds.
2. And (3) drying: and (3) carrying out moderate drying treatment on the soaked seeds to remove redundant moisture and reduce mould and bacterial pollution of the seeds after radiation.
3. And (3) sub-packaging treatment: the treated seeds are sub-packaged into radiation containers according to preset quantity and proportion so as to uniformly irradiate during radiation treatment.
S4, setting accelerator parameters
1. Determining the radiation dose: depending on the nature of the target plant and the breeding objectives, the appropriate radiation dose is determined, typically in Gray (Gy).
2. Setting radiation time: the irradiation time is set according to the radiation dose and the accelerator performance, so that the seeds can be fully irradiated.
3. Adjusting the radiation distance: according to the characteristics of the radiation equipment and the requirements of seeds, the radiation distance is adjusted, and the uniformity and the effectiveness of radiation are ensured.
S5, radiation treatment
1. Seed irradiation: and placing the pretreated seeds in radiation equipment for radiation treatment, so as to ensure uniform irradiation of the seeds in the radiation process.
2. Monitoring the irradiation process: in the radiation process, the operation state and radiation dosage of the radiation equipment are monitored in real time, and the accuracy and safety of the radiation process are ensured.
3. Recording irradiation parameters: parameters such as radiation dose, irradiation time and the like of each batch of seeds are recorded for subsequent data analysis and result evaluation.
S6, radiation post-treatment
1. Seed preservation: the irradiated seeds are stored in a suitable environment to ensure that they are not contaminated and damaged during storage.
2. Promoting seed germination: providing proper temperature and humidity conditions to promote the germination and growth of irradiated seed.
3. And (5) periodically observing the growth condition: and (3) regularly observing the growth condition of the irradiated seeds, recording the growth speed, the leaf morphology and other information, and providing data support for subsequent screening and evaluation.
S7, observing and screening
1. And (3) observing the growth condition: continuously observing the irradiated seeds, recording the growth condition and morphological characteristics, and screening plants with good growth.
2. Detecting the mutant: the plants are tested for mutant traits, such as changes in morphological, physiological or biochemical characteristics, using appropriate methods and tools.
3. Screening target characteristics: and screening mutants with target characteristics according to breeding targets, and taking the mutants as targets for subsequent reproduction and evaluation.
S8, subsequent reproduction and evaluation
1. Mutant propagation: mutants with excellent characteristics are selected for offspring propagation to expand their population size and stability.
2. Variety assessment: and (3) carrying out comprehensive evaluation on the new variety after propagation, including evaluation on growth characteristics, yield, quality and the like, so as to ensure that the new variety meets market demands.
3. Variety popularization: and (3) promoting the new variety which passes the evaluation to agricultural production practice, performing trial and demonstration promotion, and promoting the application and promotion of the new variety in the market.
Through the subdivision steps, the X-ray radiation breeding scheme can be implemented more systematically, the breeding efficiency and success rate are improved, and the plant breeding work is promoted
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. A breeding method for generating X-ray radiation by means of an electron accelerator, comprising the steps of:
s1, preparing;
S2, selecting target plant seeds;
s3, treating seeds;
S4, setting accelerator parameters;
s5, radiation treatment;
S6, radiation post-treatment;
S7, observing and screening;
S8, subsequent reproduction and evaluation.
2. A method of breeding for the production of X-ray radiation by means of an electron accelerator according to claim 1, characterized in that the preparation: the required equipment and materials are prepared, including electron accelerators, plant seeds, culture medium, growth chambers.
3. A method of breeding for the generation of X-ray radiation by means of electron accelerators according to claim 1, characterized in that the selection of target plant seeds: selecting seeds of a target plant to be bred.
4. A method of breeding for the generation of X-ray radiation by means of an electron accelerator according to claim 1, characterized in that said method comprises: seed treatment: the selected seeds are subjected to a preliminary treatment.
5. A method of breeding for the generation of X-ray radiation by means of an electron accelerator according to claim 1, characterized in that said method comprises: setting accelerator parameters: the parameters of the electron accelerator are adjusted according to the required radiation dose and energy.
6. A method of breeding for the generation of X-ray radiation by means of an electron accelerator according to claim 1, characterized in that said method comprises: radiation treatment: the prepared seeds are placed in the radiation area of the accelerator, the accelerator is started to carry out radiation treatment, and the seeds are subjected to X-ray radiation, so that DNA mutation occurs.
7. A method of breeding for the generation of X-ray radiation by means of an electron accelerator according to claim 1, characterized in that said method comprises: radiation post-treatment: the irradiated seed is removed from the irradiated area for subsequent treatment, which may include seeding on a medium, providing appropriate nutrients and environmental conditions.
8. A method of breeding for the generation of X-ray radiation by means of an electron accelerator according to claim 1, characterized in that said method comprises: observing and screening: the growth of the treated seeds is monitored to see if the desired mutation characteristics are present, and if necessary, screening can be performed to further cultivate plants having the desired characteristics.
9. A method of breeding for the generation of X-ray radiation by means of an electron accelerator according to claim 1, characterized in that said method comprises: subsequent propagation and evaluation: subsequent breeding and evaluation work is performed on the selected mutants, for example, to observe their growth traits, physiological characteristics, disease resistance, etc., to determine whether they meet breeding goals, and to further develop stable new varieties.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410541853.XA CN118202944A (en) | 2024-04-30 | 2024-04-30 | Breeding method for generating X-ray radiation through electron accelerator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410541853.XA CN118202944A (en) | 2024-04-30 | 2024-04-30 | Breeding method for generating X-ray radiation through electron accelerator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN118202944A true CN118202944A (en) | 2024-06-18 |
Family
ID=91450664
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410541853.XA Pending CN118202944A (en) | 2024-04-30 | 2024-04-30 | Breeding method for generating X-ray radiation through electron accelerator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN118202944A (en) |
-
2024
- 2024-04-30 CN CN202410541853.XA patent/CN118202944A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hohe et al. | From axenic spore germination to molecular farming: one century of bryophyte in vitro culture | |
| CN106613827A (en) | Roxburghanoectochilus factory cultivation method and cultivation module | |
| CN110622648A (en) | Alfalfa seed treatment method and cold plasma test platform | |
| CN108849528A (en) | A method of obtaining eremochloa ophiuroides mutant | |
| CN109463062A (en) | A method of utilizing atmospheric pressure at room corona treatment crop seeds | |
| CN118202944A (en) | Breeding method for generating X-ray radiation through electron accelerator | |
| CN117502222A (en) | Electron accelerator irradiation mutation breeding method and verification method thereof | |
| CN110603928B (en) | Treatment method for improving germination rate of pakchoi seeds | |
| JP2020080707A (en) | Method for producing fermented product | |
| CN105230178B (en) | Method for promoting germination of Premna fulva Craib seeds | |
| CN107686813A (en) | A kind of Euglena high-density cultivation method | |
| CN114711107A (en) | Seedling raising method for promoting elongation of mesocotyl of sweet corn and improving emergence rate | |
| KR20080073388A (en) | Mass production of bulblet via somatic embryogenic cell culture in lily | |
| CN112760397A (en) | Application of Talaromyces wortmannii in crop drought resistance and growth promotion | |
| CN105993904A (en) | Young seedling aeroponic cultivation device for synthesizing ectotrophic mycorrhiza | |
| CN114375831B (en) | Method for mutation creation of rice seedlings by heavy ion beam radiation | |
| Naser et al. | Effect of sucrose in strawberry micro-propagation using platform bioreactor under temporary immersion system | |
| Azeez et al. | Effects of pre-sowing seed treatment on the growth rate of seedlings and the activity of the excretory system of the wheat root in aquatic culture | |
| Toxirov et al. | Studying the activity of microorganisms (laboratory conditions) in moderately and highly saline meadow alluvial soils based on cotton | |
| Mishra et al. | Synergistic Effect of Different Soilless Substrates on the Hardening of In-vitro Raised Banana (Musa sp.) Saplings of Grand Naine | |
| Konstantinovna et al. | Wheat and pea seedlings as producers of biologically active compounds | |
| Sukhoparov et al. | Potato microclonal propagation method in a temporary immersion bioreactor | |
| Betskii et al. | Millimeter waves in the newest agricultural biotechnologies | |
| CN108093740A (en) | A kind of fast soiless method for culturing seedlings of rice | |
| Veni et al. | Effect of gamma rays on quantitative traits of Urdbean in M1 generation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination |