CN118165812A - In-situ filtering and collecting device for deep sea microorganisms - Google Patents
In-situ filtering and collecting device for deep sea microorganisms Download PDFInfo
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- CN118165812A CN118165812A CN202410275165.3A CN202410275165A CN118165812A CN 118165812 A CN118165812 A CN 118165812A CN 202410275165 A CN202410275165 A CN 202410275165A CN 118165812 A CN118165812 A CN 118165812A
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Abstract
The invention discloses a deep sea microorganism in-situ filtering and collecting device, which relates to the technical field of deep sea sampling and comprises a suction filtration power system, a liquid path switching system, a microorganism filtering system and a pyrolysis liquid storage bag; the suction filtration power system comprises a waterproof pump; the liquid path switching system comprises two switching valves; the switching interfaces in the two switching valves are in one-to-one correspondence, and the switching interface in one switching valve is communicated with the corresponding switching interface in the other switching valve through a connecting pipe; the microorganism filtering system comprises a plurality of filters, wherein the filters are in one-to-one correspondence with the connecting pipes; the lysate storage bag is a flexible bag; the method can carry out filtration and collection on microorganisms in deep sea in situ, and can realize filtration and collection of microorganisms in deep sea water with high time resolution by carrying out filtration and collection of microorganisms through different microorganism filtration systems.
Description
Technical Field
The invention relates to the technical field of deep sea sampling, in particular to a deep sea microorganism in-situ filtering and collecting device.
Background
Marine microorganisms are an important component of biosphere, having diverse forms and functions, including bacteria, archaea, fungi, viruses, protozoa, and the like. Marine microorganisms have great significance in maintaining marine ecosystems, affecting global climate, biotechnology applications, energy resource development, biodiversity maintenance, as ecological indicators, and the like. For example, marine microorganisms play an important role in the carbon cycle, nitrogen cycle, sulfur cycle, etc., and are an important component of the marine ecosystem. Thus, research on marine microorganisms has become one of the current hot spots of marine science research. Since marine microorganisms are huge in quantity, widely distributed and often live in extreme environments, efficient, accurate and stable sampling methods are required for collection and research of marine microorganisms.
The marine microorganism research is to obtain a large number of microorganism samples firstly, the current domestic and foreign sampling equipment is limited by the volume of a sampling tube, only a small number of microorganism samples can be obtained, and the current most deep-sea microorganism collecting devices are limited by the volume and weight, only the samples are collected and stored, but a plurality of time series samples cannot be obtained and the samples are lack of separation, so that the deep-sea specific genes and life processes cannot be continuously researched. The sampling equipment is designed in the deep sea to filter high-quality microorganism samples in the deep sea water, so that the microorganism samples can truly reflect the microorganism information in the sea water samples, and the method is an important research direction in the field of deep sea exploration.
However, no long-time-sequence, pollution-free and high-fidelity in-situ filtration sampling technology aiming at deep sea microorganisms exists at present.
Disclosure of Invention
The invention aims to provide a deep sea microorganism in-situ filtering and collecting device, which solves the problems in the prior art and realizes the filtering and collecting of microorganisms in deep sea water with high time resolution on the basis of simplifying the equipment structure.
In order to achieve the above object, the present invention provides the following solutions:
The invention provides a deep sea microorganism in-situ filtering and collecting device which comprises a suction filtration power system, a liquid path switching system, a microorganism filtering system and a pyrolysis liquid storage bag;
the suction filtration power system comprises a waterproof pump;
The liquid path switching system comprises two switching valves; the switching valve comprises a valve body, a liquid path switching valve core and a driving motor; the valve body and the liquid path switching valve core are cylindrical, the liquid path switching valve core is arranged in the valve body and is in running fit with the valve body, the circumferential side wall of the liquid path switching valve core is attached to the inner wall of the valve body, a plurality of switching interfaces which are uniformly distributed along the circumferential direction of the valve body are arranged on the circumferential side wall of the valve body, a liquid through port is arranged in the center of the side wall of the end face of the valve body, an axial blind hole and a radial hole are arranged in the liquid path switching valve core, the axial blind hole, the liquid path switching valve core and the valve body are coaxial, the opening of the axial blind hole is communicated with the liquid through port, the radial hole is formed in the way that one end of the radial hole is communicated with the axial blind hole, and the other end of the radial hole is communicated with any switching interface; the driving motor is fixedly connected with the valve body, and an output shaft of the driving motor is fixedly connected with the liquid path switching valve core; the switching interfaces in the two switching valves are in one-to-one correspondence, and the switching interface in one switching valve is communicated with the corresponding switching interface in the other switching valve through a connecting pipe; one liquid port is connected with one end of the first conduit, and the other liquid port is connected with one end of the second conduit;
The microorganism filtering system comprises a plurality of filters, wherein the filters are in one-to-one correspondence with the connecting pipes; the filter is internally provided with a filter membrane, the filter membrane divides a cavity in the filter into two subchambers, the filter is provided with a first connecting port and a second connecting port which are respectively communicated with the corresponding connecting pipes, the first connecting port is communicated with one subchamber, and the second connecting port is communicated with the other subchamber;
the lysate storage bag is a flexible bag;
when a liquid outlet of the suction filtration power system is connected with the other end of the first conduit, the other end of the second conduit is communicated with seawater; when the liquid outlet of the lysate storage bag is communicated with the other end of the second conduit, the other end of the first conduit is communicated with the sample collection tank.
Preferably, the waterproof pump comprises a gear pump, a motor for the pump and a closed cabin body, wherein the motor for the pump is fixedly arranged in the closed cabin body, an output shaft of the motor for the pump is fixedly connected with an input shaft of the gear pump, and the gear pump is fixedly connected with the closed cabin body; the sealed cabin body is provided with a first oil through hole, the first oil through hole is communicated with a first flexible oil storage bag through an oil pipe, and silicone oil is filled in the sealed cabin body and the first flexible oil storage bag.
Preferably, the liquid path switching system further comprises a housing, one valve body of the switching valve is in sealing connection with one end of the housing, the other valve body of the switching valve is in sealing connection with the other end of the housing, the housing is airtight, and the two driving motors are arranged in the housing.
Preferably, all the switching interfaces are distributed in two circles along the axial direction of the valve body, one circle of switching interfaces is a first switching interface, the other circle of switching interfaces is a second switching interface, all the first switching interfaces are uniformly distributed along the circumferential direction of the valve body, and all the second switching interfaces are uniformly distributed along the circumferential direction of the valve body; the radial holes comprise a first radial hole and a second radial hole, one end of the first radial hole is communicated with the axial blind hole, the other end of the first radial hole can be communicated with any one of the first switching interfaces, and one end of the second radial hole is communicated with the axial blind hole, and the other end of the second radial hole can be communicated with any one of the second switching interfaces.
Preferably, the filter membrane is a hydrophilic polytetrafluoroethylene filter membrane, and the pore size of the filter membrane is 0.22 mu m.
Preferably, the reversing system comprises a reversing valve and a driving device;
The reversing valve comprises a reversing valve body and a reversing shaft core, wherein a first port, a second port, a third port and a fourth port are formed in the reversing valve body, and a first flow passage, a second flow passage, a fifth port communicated with the first flow passage and a sixth port communicated with the second flow passage are formed in the reversing shaft core; the first port and the second port can both be in communication with the first flow channel, and the third port and the fourth port can both be in communication with the second flow channel; when the first port is communicated with the first flow channel, the second port is not communicated with the first flow channel, the third port is communicated with the second flow channel, and the fourth port is not communicated with the first flow channel; when the second port is communicated with the first flow channel, the first port is not communicated with the first flow channel, the fourth port is communicated with the second flow channel, and the third port is not communicated with the first flow channel;
the reversing shaft core penetrates through the reversing valve body and is in sliding fit with the reversing valve body, and the driving device is used for driving the reversing shaft core to slide relative to the reversing valve body;
The liquid outlet of the suction filtration power system is communicated with the first port, the third port is used for being communicated with seawater, the second port is communicated with the sample collection tank, and the fourth port is communicated with the liquid outlet of the pyrolysis liquid storage bag; and a pressure reducing valve is arranged on a communicating pipeline between the second port and the sample collection tank.
Preferably, the reversing shaft core is further provided with a first through flow hole, a second through flow hole, a third through flow hole and a fourth through flow hole; one end of the first through flow hole and one end of the second through flow hole are respectively communicated with the first flow channel, and one end of the third through flow hole and one end of the fourth through flow hole are respectively communicated with the second flow channel;
a first annular cavity, a second annular cavity, a third annular cavity and a fourth annular cavity which are isolated from each other are formed between the reversing shaft core and the reversing valve body, the other end of the first through flow hole is communicated with the first annular cavity, the other end of the second through flow hole is communicated with the second annular cavity, the other end of the third through flow hole is communicated with the third annular cavity, and the fourth through flow hole is communicated with the fourth annular cavity;
The first port can be in communication with the first annular cavity, the second port can be in communication with the second annular cavity, the third port can be in communication with the third annular cavity, and the fourth port can be in communication with the fourth annular cavity.
Preferably, the first annular cavity and the second annular cavity, the third annular cavity and the fourth annular cavity are respectively separated by sealing rings, and each sealing ring is fixedly connected with the reversing shaft core and is in sliding fit with the reversing valve body.
Preferably, the driving device comprises a motor cabin body fixedly connected with the reversing valve body and a reversing motor arranged in the motor cabin body, the motor cabin body is airtight, the reversing motor adopts a linear motor, the output end of the reversing motor is fixedly connected with the reversing shaft core through a connecting piece, and the connecting piece is in dynamic sealing fit with the motor cabin body.
Preferably, the motor cabin body is provided with a second oil through hole, the second oil through hole is communicated with a second flexible oil storage bag through an oil pipe, and silicone oil is filled in the motor cabin body and the second flexible oil storage bag.
Compared with the prior art, the invention has the following technical effects:
The in-situ filtering and collecting device for the deep sea microorganisms can be used for filtering and collecting the microorganisms in the deep sea in situ, and the microorganisms are filtered and collected by different microorganism filtering systems sequentially, so that the high-time-resolution filtering and collecting of the microorganisms in the deep sea water can be realized.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions of the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic structural view of a deep sea microorganism in-situ filtration collection device of the present invention;
FIG. 2 is a schematic diagram of the structure of a waterproof pump in the deep sea microorganism in-situ filtration collection device of the invention;
FIG. 3 is a schematic diagram of the reversing system in the deep sea microorganism in-situ filtration collection device of the invention;
FIG. 4 is a schematic diagram of the structure of a switching valve in the deep sea microorganism in-situ filtration and collection device of the invention;
FIG. 5 is a schematic diagram of the structure of a switching valve in the deep sea microorganism in-situ filtration and collection device of the invention;
FIG. 6 is a schematic diagram of the structure of a switching valve in the deep sea microorganism in-situ filtration and collection device of the invention;
1, a waterproof pump; 2. a reversing valve; 3. a driving device; 4. a connecting piece; 5. a lysate storage bag; 6. a sample collection canister; 7. a first conduit; 8. a first adapter; 9. a liquid path switching valve core; 10. a valve body; 11. a one-way thrust cylindrical roller bearing; 12. a housing; 13. an output shaft of the motor; 14. a driving motor; 15. a second adapter; 16. a second conduit; 17. a filter; 18. a water-tight connector interface; 19. a support rod; 20. a housing end cap; 21. a valve body end cover; 22. a liquid port; 23. an axial blind hole; 24. a first radial bore; 25. a second radial bore; 26. a first switching interface; 27. a second switching interface;
101. A gear pump; 102. a closed cabin body; 103. a first oil port;
201. A reversing valve body; 202. a reversing shaft core; 203. a first port; 204. a second port; 205. a third port; 206. a fourth port; 207. a fifth port; 208. a sixth port; 209. a first flow passage; 210. a first through-flow hole; 211. a second vent; 212. a second flow passage; 213. a third flow-through hole; 214. a fourth flow hole;
301. a motor compartment; 302. reversing the motor; 303. and a second oil through port.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention aims to provide a deep sea microorganism in-situ filtering and collecting device, which solves the problems in the prior art and realizes the filtering and collecting of microorganisms in deep sea water with high time resolution on the basis of simplifying the equipment structure.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
As shown in fig. 1-6, the embodiment provides a deep sea microorganism in-situ filtration collection device, which comprises a suction filtration power system, a reversing system, a liquid path switching system, a microorganism filtration system and a lysate storage bag 5.
Wherein, the suction filtration power system comprises a waterproof pump 1; in this embodiment, the waterproof pump 1 specifically includes a gear pump 101, a pump motor and a closed cabin 102, the pump motor is fixedly arranged in the closed cabin 102, an output shaft of the pump motor is fixedly connected with an input shaft of the gear pump 101, and the gear pump 101 is fixedly connected with the closed cabin 102; the airtight cabin body 102 is provided with a first oil through hole 103, the first oil through hole 103 is communicated with a first flexible oil storage bag through an oil pipe, and silicone oil is filled in the airtight cabin body 102 and the first flexible oil storage bag. The motor for the pump adopts a brushless direct current motor, and the purpose of arranging the first flexible oil storage bag and the airtight cabin body 102 is to balance the pressure inside and outside the airtight cabin body 102, because the use scene of the in-situ filtering and collecting device for the deep sea microorganisms is in deep sea, the water pressure of the deep sea is very large, if the airtight cabin body 102 is airtight air, the pressure difference between the inside and the outside of the airtight cabin body 102 is very large, and the first flexible oil storage bag is flexible and can bear the water pressure of the sea bottom, and the silicone oil in the airtight cabin body 102 has the pressure balanced with the water pressure of the sea bottom, so that the pressure difference between the inside and the outside of the airtight cabin body 102 is reduced, the wall thickness of the airtight cabin body 102 is reduced, and the manufacturing cost is reduced.
The liquid path switching system comprises two switching valves; the switching valve comprises a valve body 10, a liquid path switching valve core 9 and a driving motor 14; the valve body 10 and the liquid way switching valve core 9 are cylindrical, the liquid way switching valve core 9 is arranged in the valve body 10 and is in running fit with the valve body 10, the circumferential side wall of the liquid way switching valve core 9 is attached to the inner wall of the valve body 10, and meanwhile, the upper end face and the lower end face of the liquid way switching valve core 9 are respectively provided with one-way thrust cylindrical roller bearings for reducing the rotating friction force between the liquid way switching valve core 9 and the shell end cover 20 and between the liquid way switching valve core 9 and the valve body end cover 21.
The valve body 10 is provided with 80 switching interfaces uniformly distributed along the circumferential direction of the valve body 10 on the circumferential side wall, the center of the end face side wall of the valve body 10 is provided with a liquid through hole 22, an axial blind hole 23 and a radial hole are arranged in the liquid path switching valve core 9, the axial blind hole 23, the liquid path switching valve core 9 and the valve body 10 are coaxial, the opening of the axial blind hole 23 is communicated with the liquid through hole 22, the radial hole is communicated with the axial blind hole 23 at one end and can be communicated with any switching interface at the other end; a motor output shaft 13 of a driving motor 14 is fixedly connected with the valve body 10, and an output shaft of the driving motor 14 is fixedly connected with the liquid path switching valve core 9; the switching interfaces in the two switching valves are in one-to-one correspondence, and the switching interface in one switching valve is communicated with the corresponding switching interface in the other switching valve through a connecting pipe; one fluid port 22 is connected to one end of the first conduit 7 through the first adapter 8, and the other fluid port 22 is connected to one end of the second conduit 16 through the second adapter 15.
In this embodiment, the liquid path switching system further includes a casing 12, the casing 12 is cylindrical, two ends of the casing 12 are respectively provided with end covers of the casing 12, a valve body 10 of one switching valve is connected with one end of the casing 12 in a sealing manner, a valve body 10 of the other switching valve is connected with the other end of the casing 12 in a sealing manner, the casing 12 is airtight, and two driving motors 14 are both disposed in the casing 12. A watertight connector interface 18 is provided on the housing 12, and the drive motor 14 is connected to an external power supply through the watertight connector interface 18.
All the switching interfaces are distributed in two circles along the axial direction of the valve body 10, one circle of switching interfaces is a first switching interface 26, the other circle of switching interfaces is a second switching interface 27, all the first switching interfaces 26 are uniformly distributed along the circumferential direction of the valve body 10, and all the second switching interfaces 27 are uniformly distributed along the circumferential direction of the valve body 10; the radial holes comprise a first radial hole 24 and a second radial hole 25, wherein one end of the first radial hole 24 is communicated with the axial blind hole 23, the other end of the first radial hole can be communicated with any one of the first switching interfaces 26, one end of the second radial hole 25 is communicated with the axial blind hole 23, and the other end of the second radial hole can be communicated with any one of the second switching interfaces 27.
The microorganism filtering system comprises a plurality of filters 17, wherein the filters 17 are in one-to-one correspondence with the connecting pipes; the filter 17 is provided with a filter membrane, the filter membrane divides a cavity in the filter 17 into two subchambers, the filter 17 is provided with a first connecting port and a second connecting port which are respectively communicated with corresponding connecting pipes, the first connecting port is communicated with one subchamber, and the second connecting port is communicated with the other subchamber; the filter membrane adopts a hydrophilic polytetrafluoroethylene filter membrane, and the pore diameter of the filter membrane is 0.22 mu m.
The pyrolysis liquid storage bag 5 is a flexible bag, such as a flexible polymer plastic water bag, and pyrolysis liquid is stored in the pyrolysis liquid storage bag 5;
when the liquid outlet of the suction filtration power system is connected with the other end of the first conduit 7, the other end of the second conduit 16 is communicated with seawater; when the liquid outlet of the lysate storage bag 5 is communicated with the other end of the second conduit 16, the other end of the first conduit 7 is communicated with the sample collection tank 6. Specifically, in this embodiment, the switching between the two connection modes is implemented through the reversing system.
The reversing system comprises a reversing valve 2 and a driving device 3; the reversing valve 2 comprises a reversing valve body 201 and a reversing shaft core 202, wherein a first port 203, a second port 204, a third port 205 and a fourth port 206 are formed in the reversing valve body 201, and a first flow passage 209, a second flow passage 212, a fifth port 207 communicated with the first flow passage 209 and a sixth port 208 communicated with the second flow passage 212 are formed in the reversing shaft core 202; both the first port 203 and the second port 204 can communicate with the first flow channel 209, and both the third port 205 and the fourth port 206 can communicate with the second flow channel 212; when the first port 203 is communicated with the first flow channel 209, the second port 204 is not communicated with the first flow channel 209, the third port 205 is communicated with the second flow channel 212, and the fourth port 206 is not communicated with the first flow channel 209; when the second port 204 is in communication with the first flow channel 209, the first port 203 is not in communication with the first flow channel 209, the fourth port 206 is in communication with the second flow channel 212, and the third port 205 is not in communication with the first flow channel 209; the reversing shaft core 202 passes through the reversing valve body 201 and is in sliding fit with the reversing valve body 201, and the driving device 3 is used for driving the reversing shaft core 202 to slide relative to the reversing valve body 201;
The liquid outlet of the suction filtration power system is communicated with the first port 203, the third port 205 is used for communicating with sea water, the second port 204 is communicated with the sample collection tank 6, and the fourth port 206 is communicated with the liquid outlet of the pyrolysis liquid storage bag 5; and a pressure reducing valve is provided on the communication line between the second port 204 and the sample collection canister 6.
The reversing shaft core 202 is also provided with a first through-flow hole 210, a second through-flow hole 211, a third through-flow hole 213 and a fourth through-flow hole 214; one end of the first through-flow hole 210 and one end of the second through-flow hole 211 are respectively communicated with the first flow channel 209, and one end of the third through-flow hole 213 and one end of the fourth through-flow hole 214 are respectively communicated with the second flow channel 212;
A first annular cavity, a second annular cavity, a third annular cavity and a fourth annular cavity which are isolated from each other are formed between the reversing shaft core 202 and the reversing valve body 201, the other end of the first through-flow hole 210 is communicated with the first annular cavity, the other end of the second through-flow hole 211 is communicated with the second annular cavity, the other end of the third through-flow hole 213 is communicated with the third annular cavity, and the fourth through-flow hole 214 is communicated with the fourth annular cavity; the first port 203 can communicate with a first annular chamber, the second port 204 can communicate with a second annular chamber, the third port 205 can communicate with a third annular chamber, and the fourth port 206 can communicate with a fourth annular chamber.
The first annular cavity and the second annular cavity, and the third annular cavity and the fourth annular cavity are respectively separated by sealing rings, and each sealing ring is fixedly connected with the reversing shaft core 202 and is in sliding fit with the reversing valve body 201.
The driving device 3 comprises a motor cabin 301 fixedly connected with the reversing valve body 201 through a supporting rod 19 and a reversing motor 302 arranged in the motor cabin 301, the motor cabin 301 is airtight, the reversing motor 302 adopts a linear motor, the output end of the reversing motor 302 is fixedly connected with the reversing shaft core 202 through a connecting piece 4, and the connecting piece 4 is in dynamic sealing fit with the motor cabin 301. The motor compartment 301 is provided with a watertight connector interface 18, and the reversing motor 302 is connected with an external power supply through the watertight connector interface 18 on the motor compartment 301.
The motor cabin 301 is provided with a second oil through hole 303, the second oil through hole 303 is communicated with a second flexible oil storage bag through an oil pipe, and silicone oil is filled in the motor cabin 301 and the second flexible oil storage bag; the purpose of providing the second flexible oil storage bag and the motor compartment 301 is to balance the pressure inside and outside the motor compartment 301, because the usage scenario of the deep sea microorganism in-situ filtering and collecting device in this embodiment is in deep sea, if the motor compartment 301 is sealed with air, the pressure difference between the inside and the outside of the motor compartment 301 is large, and the second flexible oil storage bag is flexible and can be subjected to the water pressure of the sea floor, and the silicone oil in the motor compartment 301 has the pressure balanced with the water pressure of the sea floor, so that the pressure difference between the inside and the outside of the motor compartment 301 is reduced, which is also beneficial to reducing the wall thickness of the motor compartment 301 and reducing the manufacturing cost.
The specific use method of the deep-sea microorganism in-situ filtering and collecting device is as follows:
Firstly, the deep-sea microorganism in-situ filtering and collecting device can be directly connected with a mother ship power supply cable to perform independent sampling work in deep sea water, meanwhile, deep-sea water suction filtration and sampling can be performed by carrying equipment such as a deep-sea moving platform, an ROV and the like, before the deep-sea microorganism in-situ filtering and collecting device is operated, the deep-sea microorganism in-situ filtering and collecting device is required to be distributed to a point where a sample needs to be filtered, and after the surrounding sea water environment is stable, the deep-sea microorganism in-situ filtering and collecting device is connected with an upper computer to automatically operate;
when the deep sea microorganism in-situ filtration collection device of the embodiment is in an initial state, the liquid path switching valve core 9 is in an initial in-situ state, the reversing shaft core 202 is in an extended state (a state that a first oil port is communicated with the first flow channel 209 and a third oil port is communicated with the second flow channel 212), and the gear pump 101 does not work;
when the first sampling time and sampling point are reached: (1) The liquid path switching valve core 9 is rotatably positioned at the interface N, the reversing shaft core 202 is continuously in an extending state (a state that a first oil port is communicated with the first flow channel 209 and a third oil port is communicated with the second flow channel 212), and the suction filtration power system starts to work, and at the moment, the device is in an in-situ filtration process; (2) After 30 minutes, the in-situ filtering action is completed, the liquid path switching valve core 9 is continuously positioned at the interface N, and at the moment, the suction filtration power system stops working; when the suction filtration power system stops working, the reversing shaft core 202 is controlled to be switched to a retracted state (a state that a second oil port is communicated with the first flow channel 209 and a fourth oil port is communicated with the second flow channel 212), after the reversing shaft core 202 is switched to be in place, under the action of pressure difference, a cracking liquid in a cracking liquid storage cabin backflushes the filter 17, and the backflushed enrichment liquid flows through a pressure reducing valve and is finally conveyed to the sample collection tank 6, and at the moment, the device is in the process of in-situ cracking and pressure reducing quantitative cabin feeding; (3) After the quantitative cabin-entering action is finished, the liquid path switching valve core 9 is switched to an initial original position, the reversing shaft core 202 is controlled to extend to an extending state (a state that a first oil port is communicated with the first flow channel 209 and a third oil port is communicated with the second flow channel 212), the suction filtration power system is controlled to work, the pipeline flushing action is carried out, and after five minutes, the suction filtration power system stops working and waits for a second sampling time (sampling point) to act.
Reaching the second sampling time and sampling point: (1) The liquid path switching valve core 9 is rotatably positioned at the interface n+1, the reversing shaft core 202 is continuously in an extending state (a state that a first oil port is communicated with the first flow channel 209 and a third oil port is communicated with the second flow channel 212), and the suction filtration power system starts to work, and at the moment, the device is in an in-situ filtration process; (2) After 30 minutes, the in-situ filtering action is completed, the liquid path switching valve core 9 is continuously positioned at the interface N+1, and at the moment, the suction filtration power system stops working; when the suction filtration power system stops working, the reversing shaft core 202 is controlled to be switched to a retracted state (a state that a second oil port is communicated with the first flow channel 209 and a fourth oil port is communicated with the second flow channel 212), after the reversing shaft core 202 is switched to be in place, under the action of pressure difference, a cracking liquid in a cracking liquid storage cabin backflushes the filter 17, and the backflushed enrichment liquid flows through a pressure reducing valve and is finally conveyed to the sample collection tank 6, and at the moment, the device is in the process of in-situ cracking and pressure reducing quantitative cabin feeding; (3) After the quantitative cabin-entering action is finished, the liquid path switching valve core 9 is switched to an initial original position, the reversing shaft core 202 is controlled to extend to an extending state (a state that a first oil port is communicated with the first flow channel 209 and a third oil port is communicated with the second flow channel 212), the suction filtration power system is controlled to work, the pipeline flushing action is carried out, after five minutes, the suction filtration power system stops working, and the action of a third sampling time (sampling point) is waited. And so on, 79 sampling actions are completed. In the whole process, the upper and lower switching valve cores 9 of the liquid path switching system synchronously act, so that the switching joint in one switching valve is ensured to be communicated with the corresponding switching joint in the other switching valve.
When the sampling operation is finished or the sampling of 79 sampling channels is finished, the liquid path switching valve core 9 is switched to the original position, the reversing shaft core 202 is in an extending state (a state that the first oil port is communicated with the first flow channel 209 and the third oil port is communicated with the second flow channel 212), the gear pump 101 does not work, and the recovery device is waited for.
It should be noted that, the deep-sea microorganism in-situ filtration and collection device of the embodiment can sample underwater for a long time, and in order to prevent corrosion of the cabin body by the seawater extreme environment, each cabin body part is made of titanium alloy materials. In order to reduce the pollution of the sampling device to microorganisms in seawater, each liquid path connecting conduit, the filter shell and each connecting joint are made of high-temperature resistant thermoplastic PEEK materials.
The principles and embodiments of the present invention have been described in detail with reference to specific examples, which are provided to facilitate understanding of the method and core ideas of the present invention; also, it is within the scope of the present invention to be modified by those of ordinary skill in the art in light of the present teachings. In view of the foregoing, this description should not be construed as limiting the invention.
Claims (10)
1. The utility model provides a deep sea microorganism normal position filters collection system which characterized in that: comprises a suction filtration power system, a liquid path switching system, a microorganism filtration system and a pyrolysis liquid storage bag;
the suction filtration power system comprises a waterproof pump;
The liquid path switching system comprises two switching valves; the switching valve comprises a valve body, a liquid path switching valve core and a driving motor; the valve body and the liquid path switching valve core are cylindrical, the liquid path switching valve core is arranged in the valve body and is in running fit with the valve body, the circumferential side wall of the liquid path switching valve core is attached to the inner wall of the valve body, a plurality of switching interfaces which are uniformly distributed along the circumferential direction of the valve body are arranged on the circumferential side wall of the valve body, a liquid through port is arranged in the center of the side wall of the end face of the valve body, an axial blind hole and a radial hole are arranged in the liquid path switching valve core, the axial blind hole, the liquid path switching valve core and the valve body are coaxial, the opening of the axial blind hole is communicated with the liquid through port, the radial hole is formed in the way that one end of the radial hole is communicated with the axial blind hole, and the other end of the radial hole is communicated with any switching interface; the driving motor is fixedly connected with the valve body, and an output shaft of the driving motor is fixedly connected with the liquid path switching valve core; the switching interfaces in the two switching valves are in one-to-one correspondence, and the switching interface in one switching valve is communicated with the corresponding switching interface in the other switching valve through a connecting pipe; one liquid port is connected with one end of the first conduit, and the other liquid port is connected with one end of the second conduit;
The microorganism filtering system comprises a plurality of filters, wherein the filters are in one-to-one correspondence with the connecting pipes; the filter is internally provided with a filter membrane, the filter membrane divides a cavity in the filter into two subchambers, the filter is provided with a first connecting port and a second connecting port which are respectively communicated with the corresponding connecting pipes, the first connecting port is communicated with one subchamber, and the second connecting port is communicated with the other subchamber;
the lysate storage bag is a flexible bag;
when a liquid outlet of the suction filtration power system is connected with the other end of the first conduit, the other end of the second conduit is communicated with seawater; when the liquid outlet of the lysate storage bag is communicated with the other end of the second conduit, the other end of the first conduit is communicated with the sample collection tank.
2. The deep sea microorganism in-situ filtration collection device of claim 1, wherein: the waterproof pump comprises a gear pump, a motor for the pump and a closed cabin body, wherein the motor for the pump is fixedly arranged in the closed cabin body, an output shaft of the motor for the pump is fixedly connected with an input shaft of the gear pump, and the gear pump is fixedly connected with the closed cabin body; the sealed cabin body is provided with a first oil through hole, the first oil through hole is communicated with a first flexible oil storage bag through an oil pipe, and silicone oil is filled in the sealed cabin body and the first flexible oil storage bag.
3. The deep sea microorganism in-situ filtration collection device of claim 1, wherein: the liquid path switching system further comprises a shell, one valve body of the switching valve is in sealing connection with one end of the shell, the other valve body of the switching valve is in sealing connection with the other end of the shell, the shell is airtight, and the two driving motors are arranged in the shell.
4. The deep sea microorganism in-situ filtration collection device of claim 1, wherein: all the switching interfaces are distributed in two circles along the axial direction of the valve body, one circle of switching interfaces is a first switching interface, the other circle of switching interfaces is a second switching interface, all the first switching interfaces are uniformly distributed along the circumferential direction of the valve body, and all the second switching interfaces are uniformly distributed along the circumferential direction of the valve body; the radial holes comprise a first radial hole and a second radial hole, one end of the first radial hole is communicated with the axial blind hole, the other end of the first radial hole can be communicated with any one of the first switching interfaces, and one end of the second radial hole is communicated with the axial blind hole, and the other end of the second radial hole can be communicated with any one of the second switching interfaces.
5. The deep sea microorganism in-situ filtration collection device of claim 1, wherein: the filter membrane adopts a hydrophilic polytetrafluoroethylene filter membrane, and the pore diameter of the filter membrane is 0.22 mu m.
6. The deep sea microorganism in-situ filtration collection device of claim 1, wherein: the reversing system comprises a reversing valve and a driving device;
The reversing valve comprises a reversing valve body and a reversing shaft core, wherein a first port, a second port, a third port and a fourth port are formed in the reversing valve body, and a first flow passage, a second flow passage, a fifth port communicated with the first flow passage and a sixth port communicated with the second flow passage are formed in the reversing shaft core; the first port and the second port can both be in communication with the first flow channel, and the third port and the fourth port can both be in communication with the second flow channel; when the first port is communicated with the first flow channel, the second port is not communicated with the first flow channel, the third port is communicated with the second flow channel, and the fourth port is not communicated with the first flow channel; when the second port is communicated with the first flow channel, the first port is not communicated with the first flow channel, the fourth port is communicated with the second flow channel, and the third port is not communicated with the first flow channel;
the reversing shaft core penetrates through the reversing valve body and is in sliding fit with the reversing valve body, and the driving device is used for driving the reversing shaft core to slide relative to the reversing valve body;
The liquid outlet of the suction filtration power system is communicated with the first port, the third port is used for being communicated with seawater, the second port is communicated with the sample collection tank, and the fourth port is communicated with the liquid outlet of the pyrolysis liquid storage bag; and a pressure reducing valve is arranged on a communicating pipeline between the second port and the sample collection tank.
7. The deep sea microorganism in-situ filtration collection device of claim 6, wherein: the reversing shaft core is also provided with a first through hole, a second through hole, a third through hole and a fourth through hole; one end of the first through flow hole and one end of the second through flow hole are respectively communicated with the first flow channel, and one end of the third through flow hole and one end of the fourth through flow hole are respectively communicated with the second flow channel;
a first annular cavity, a second annular cavity, a third annular cavity and a fourth annular cavity which are isolated from each other are formed between the reversing shaft core and the reversing valve body, the other end of the first through flow hole is communicated with the first annular cavity, the other end of the second through flow hole is communicated with the second annular cavity, the other end of the third through flow hole is communicated with the third annular cavity, and the fourth through flow hole is communicated with the fourth annular cavity;
The first port can be in communication with the first annular cavity, the second port can be in communication with the second annular cavity, the third port can be in communication with the third annular cavity, and the fourth port can be in communication with the fourth annular cavity.
8. The deep sea microorganism in-situ filtration collection device of claim 7, wherein: the first annular cavity and the second annular cavity, the third annular cavity and the fourth annular cavity are respectively separated by sealing rings, and each sealing ring is fixedly connected with the reversing shaft core and is in sliding fit with the reversing valve body.
9. The deep sea microorganism in-situ filtration collection device of claim 6, wherein: the driving device comprises a motor cabin body fixedly connected with the reversing valve body and a reversing motor arranged in the motor cabin body, the motor cabin body is airtight, the reversing motor adopts a linear motor, the output end of the reversing motor is fixedly connected with the reversing shaft core through a connecting piece, and the connecting piece is in dynamic sealing fit with the motor cabin body.
10. The deep sea microorganism in-situ filtration collection device of claim 9, wherein: the motor cabin body is provided with a second oil through hole, the second oil through hole is communicated with a second flexible oil storage bag through an oil pipe, and silicone oil is filled in the motor cabin body and the second flexible oil storage bag.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410275165.3A CN118165812A (en) | 2024-03-12 | 2024-03-12 | In-situ filtering and collecting device for deep sea microorganisms |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202410275165.3A CN118165812A (en) | 2024-03-12 | 2024-03-12 | In-situ filtering and collecting device for deep sea microorganisms |
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| CN202410275165.3A Pending CN118165812A (en) | 2024-03-12 | 2024-03-12 | In-situ filtering and collecting device for deep sea microorganisms |
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