CN117983657B - Method for cadmium pollution repair and effect evaluation and cadmium pollution repair method - Google Patents
Method for cadmium pollution repair and effect evaluation and cadmium pollution repair method Download PDFInfo
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Abstract
The invention provides a method for cadmium pollution repair and effect evaluation and a cadmium pollution repair method, which comprises the following steps: inoculating a kidney worm in a cadmium polluted environment, and treating cadmium in the environment by utilizing the kidney worm to repair cadmium pollution; recording the initial inoculation density of the reniform worm population, and then measuring the population growth rate of the reniform worm, the bioaccumulation amount of the reniform worm cells to cadmium and the metallothionein content of the reniform worm along with the treatment time; the repair results of cadmium contamination using reniform worms were predicted and evaluated according to the following formula: . The invention utilizes the bioaccumulation effect and the environmental removal capability of the kidney-shaped insects under the exposure of cadmium with different concentrations, and repairs cadmium pollution through the kidney-shaped insects, thereby having good repair efficiency, economy and predictability.
Description
Technical Field
The invention relates to the technical field of bioremediation and evaluation of heavy metal pollution, in particular to a method for cadmium pollution remediation and effect evaluation and a cadmium pollution remediation method.
Background
As an environmental friendly and sustainable cadmium pollution treatment method, the previous research on the environmental cadmium pollution bioremediation technology mainly uses the synergistic effect of microorganisms, plants or both to remove or stabilize cadmium pollution in the environment, and the following remediation forms generally exist:
Microbial remediation: by utilizing the natural heavy metal tolerance and accumulation capability of certain bacteria, fungi and algae, cadmium in water and soil can be removed through mechanisms such as biological adsorption, biological enrichment, biological transformation, biological precipitation and the like. These microorganisms are able to convert cadmium in dissolved form into insoluble or less active form, thereby reducing its bioavailability and toxicity; phytoremediation (plant extraction): cadmium in soil is removed by using plants capable of absorbing and accumulating heavy metals from the soil. These plants absorb cadmium in the soil to the roots during growth and then transfer to the stems and leaves, reducing the cadmium content of the soil by harvesting these parts; mycorrhiza repair: certain mycorrhizal fungi can form symbiotic relation with plant root systems, and the absorption and tolerance of the plants to cadmium are enhanced. These fungi not only assist the plant in moisture and nutrition, but also limit cadmium uptake by forming a protective barrier in the rhizosphere area.
Bioremediation technology has shown great potential in environmental cadmium pollution remediation, but still faces some problems and limitations in practical application processes: the cost based on large plants or microorganism-plant combined repair is high, the reaction time is long, the operation is complex, the influence factors are many, and the method is limited in practical application; the introduction of foreign microorganisms or plants for cadmium pollution remediation can also generate the risk problems of species invasion, biodiversity reduction and the like of a local ecological system; when the pollution of low concentration cadmium is treated, the repairing efficiency and economy of large plants and microorganisms are greatly reduced.
Meanwhile, the predictability and effectiveness evaluation of the existing bioremediation process are difficult, the bioremediation effect under most conditions is often uncertain, and a quantitative evaluation scheme for the bioremediation effect of cadmium pollution cannot be established.
Disclosure of Invention
In order to solve the problems of long reaction time, complex operation, multiple influence factors and risk problems, low repair efficiency and economy and difficult predictability and effectiveness evaluation of the existing bioremediation process in the environmental cadmium pollution treatment of the bioremediation in the prior art, the invention provides a method for cadmium pollution repair and effect evaluation and a cadmium pollution repair method.
The technical problems of the invention are solved by the following technical scheme:
a method for cadmium pollution remediation and effect assessment, comprising:
inoculating a kidney worm in a cadmium polluted environment, and treating cadmium in the environment by utilizing the kidney worm to repair cadmium pollution; recording the initial inoculation density of the reniform worm population, and measuring the population growth rate of the reniform worm, the bioaccumulation of the reniform worm cells to cadmium and the metallothionein content of the reniform worm along with the treatment time;
The repair results of cadmium contamination using reniform worms were predicted and evaluated according to the following formula:
Wherein, For initial inoculation density of the reniform pest population,/>Is the population growth rate of reniform worms/(For processing time,/>For/>Time accumulation of reniform cells on cadmium,/>For/>The change amount of the cadmium concentration of the internal and external environments of the time reniform worms; wherein/>And calculating according to a dose effect regression equation of the cadmium accumulation amount and the metallothionein content of the reniform worms corresponding to the treatment time.
In some embodiments, at times t of 24h, 48h, 72h, 96h, the dose-effect regression equations for cadmium accumulation and metallothionein content of corresponding reniform insect cells are as follows:
24 h： Y=4.69X0.16, R2=0.86
48 h： Y=4.29X0.18, R2=0.83
72 h： Y=3.82X0.17, R2=0.91
96 h： Y=3.33X0.15, R2=0.83
Wherein Y represents the dependent variable of the regression equation, X represents the independent variable of the regression equation, and R 2 is the coefficient of determination of the regression equation.
The invention also provides a method for repairing cadmium pollution, which comprises inoculating a kidney worm in a cadmium pollution environment, and treating cadmium in the environment by utilizing the kidney worm to repair the cadmium pollution; wherein, based on the planned cadmium removal target, the initial inoculation density of the reniform worm population is adjusted according to the following formula:
Wherein, For initial inoculation density of the reniform pest population,/>Is the population growth rate of reniform worms/(For processing time,/>For/>Time accumulation of reniform cells on cadmium,/>For/>The change amount of the cadmium concentration of the internal and external environments of the time reniform worms; wherein/>And calculating according to a dose effect regression equation of the cadmium accumulation amount and the metallothionein content of the reniform worms corresponding to the treatment time.
In some embodiments, at times t of 24h, 48h, 72h, 96h, the dose-effect regression equations for cadmium accumulation and metallothionein content of corresponding reniform insect cells are as follows:
24 h： Y=4.69X0.16, R2=0.86
48 h： Y=4.29X0.18, R2=0.83
72 h： Y=3.82X0.17, R2=0.91
96 h： Y=3.33X0.15, R2=0.83
Wherein Y represents the dependent variable of the regression equation, X represents the independent variable of the regression equation, and R 2 is the coefficient of determination of the regression equation.
In some embodiments, cadmium is removed from contaminated water having an environmental cadmium concentration of less than 24.85 μg/L within 96 hours.
In some embodiments, further comprising: the method for cadmium pollution repair and effect evaluation is used for predicting and evaluating the repair effect of environmental cadmium.
In some embodiments, further comprising: when the reniform worm cadmium is exposed for a preset time, the population density of the reniform worm is measured, and the environmental cadmium concentration is determined according to a dose effect regression equation of the environmental cadmium concentration and the reniform worm population density exposed for the preset time.
In some embodiments, at predetermined times of 24h, 48h, 72h, 96h, the corresponding dose effect regression equations for the environmental cadmium concentration and the reniform worm population density are as follows:
24 h： Y=0.84X2-58.52X+1579.54, R2=0.98
48 h： Y=2.14X2-120.48X+2267.45, R2=0.97
72 h： Y=16.68X2-618.70X+7150.25, R2=0.94
96 h： Y=23.72X2-1003.47X+13973.64, R2=0.88
Wherein Y represents the dependent variable of the regression equation, X represents the independent variable of the regression equation, and R 2 is the coefficient of determination of the regression equation.
In some embodiments, the inoculating the reniform worm in a cadmium contaminated environment comprises adding a dormant capsule of the reniform worm to the cadmium contaminated soil.
The beneficial effects of the invention include:
According to the method for cadmium pollution repair and effect evaluation and the cadmium pollution repair method, the kidney-shaped insects are inoculated in a cadmium pollution environment, cadmium in the environment is treated by utilizing the kidney-shaped insects to repair cadmium pollution, the repair result of repairing the cadmium pollution by using the kidney-shaped insects is predicted and evaluated, an environment cadmium pollution repair strategy is designed, the defect of adopting large plants or microorganism-plant combined repair is avoided, the repair cost is low, the reaction time is short, the operation is simple and convenient, the influence factors and the risk problems are few, and the cadmium is treated by the kidney-shaped insects, so that the method has good repair efficiency, economy and predictability.
The invention reveals and utilizes the unique properties of the bioaccumulation effect and the environmental removal capability of the reniform worms under the exposure of cadmium with different concentrations, and is an important tool in developing and applying a heavy metal polluted water-soil restoration strategy based on biotechnology; the specific mechanism of the reniform worms for relieving the adverse effect of cadmium stress by increasing the content of metallothionein is found, and an effective means is provided for a heavy metal pollution treatment strategy based on a bioremediation technology; the invention clearly verifies the function and the potential of the reniform worm for treating cadmium, builds a predictive mathematical model formula through a corresponding regression equation, and provides a new method for solving the problem of cadmium pollution to the environment.
Other advantages of embodiments of the present invention are further described below.
Drawings
FIG. 1 is a plot of the population density variation of reniform worms over cadmium exposure time in an embodiment of the invention.
Detailed Description
The following describes embodiments of the present invention in detail. It should be emphasized that the following description is merely exemplary in nature and is in no way intended to limit the scope of the invention or its applications.
It should be noted that, in this embodiment, the terms of left, right, upper, lower, top, bottom, etc. are merely relative terms, or refer to the normal use state of the product, and should not be considered as limiting.
The embodiment of the invention provides a method for cadmium pollution repair and effect evaluation and a cadmium pollution repair method, which comprises the following steps: inoculating a kidney worm in a cadmium polluted environment, and treating cadmium in the environment by utilizing the kidney worm to repair cadmium pollution; recording the initial inoculation density of the reniform worm population, and measuring the population growth rate of the reniform worm, the bioaccumulation of the reniform worm cells to cadmium and the metallothionein content of the reniform worm along with the treatment time; the repair results of cadmium contamination using reniform worms were predicted and evaluated according to the following formula: ; wherein K is the initial inoculation density of the reniform worm population, a is the population growth rate of the reniform worm, t is the treatment time,/> For the bioaccumulation of cadmium by kidney worm cells at time t,/>The change amount of the cadmium concentration in the internal and external environments of the reniform worms at time t; wherein/>According to a dose effect regression equation of the cadmium accumulation amount and the metallothionein content of the reniform worms corresponding to the treatment time; meanwhile, the cadmium pollution repairing method comprises inoculating a kidney worm in a cadmium pollution environment, and treating cadmium in the environment by utilizing the kidney worm to repair cadmium pollution; wherein the cadmium removal target is planned based and according to the formulaTo adjust the initial inoculation density of the reniform pest population.
In the embodiment of the invention, a novel cadmium pollution repair biotechnology is established by utilizing the tolerance mechanism and the bioaccumulation effect of soil kidney-shaped insects on cadmium, and a mathematical model is established based on key biological parameters and is used for synchronously predicting and evaluating the cadmium pollution repair effect.
A. the following describes the materials and methods in the embodiments of the present invention:
(i) Standardized culture of reniform worms
The reniform worms (Colpoda sp.) used in the experiments in the examples of the invention are derived from the university of Harbin industry (Shenzhen) municipal water resource and water environment national emphasis laboratory reniform worm strain preservation center. Individual cells were carefully selected from the mixed culture under a microscope using a micropipette. In deionized water medium enriched with sterilized wheat kernels as a nutrient source, a monoclonal culture was established and placed in 6-well plates. Culturing in a constant temperature and humidity incubator at 25deg.C with light/dark cycle of 12 hr and light intensity of 600 Lux.
(Ii) Extracellular/intracellular cadmium concentration determination
The culture solutions of cadmium (serial numbers: CK-0, L1-0.34. Mu.g/L, L-0.67. Mu.g/L, L-1.01. Mu.g/L, H-8.28. Mu.g/L, H-16.57. Mu.g/L, H-24.85. Mu.g/L) with different concentration gradients were inoculated for experiments, and the kidney worm cells and the external culture solutions of the experimental group and the control group were collected every 24 hours during the experiment period of 96 hours. After centrifugation through 4000 rpm min, the supernatant was collected and filtered through a 0.22 μm filter to obtain an extracellular culture broth sample. The precipitate was washed twice with ultrapure water, then freeze-dried in a vacuum freeze-dryer for 3 hours, and weighed to calculate the dry weight of the cells, thereby obtaining the mass of the intracellular sample. The obtained sample is digested by hydrochloric acid and nitric acid, the cadmium content is measured by using an inductively coupled plasma atomic emission spectrometer (iCAP Pro, thermo Scientific, USA), the sample is processed according to the standard of national food safety Standard-determination of cadmium in food (GB 5009.15-2014), the cadmium concentration of the kidney worm in vitro culture solution (Ext-Cd) is expressed in mug/L, and the accumulation of cadmium in kidney worm cells (Bio-Cd) is expressed in mug/g.
(Iii) Metallothionein detection
The reniform worm broth was collected every 24 hours during 96 hours of cadmium exposure, and washed three times with Phosphate Buffered Saline (PBS) to remove impurities such as bacteria and culture medium. After centrifugation at 4000 rpm min at 4 ℃, the samples were concentrated to a cell density of 1 x 106 ind/ml, stored in 1.5ml centrifuge tubes to-80 ℃ refrigerator, cadmium metallothionein (Cd-MTs) content was determined using enzyme-linked immunosorbent assay (ELISA) kit, total protein content of each sample was quantified using BCA protein quantification kit (Beyotime co., china) according to manufacturer's instructions, and nine independent biological replicates were performed for all exposure groups.
(Iii) statistical analysis
Cadmium removal rate (CR) of reniform worms was calculated using the following equation
In the above formula, CR represents the cadmium removal rate (%) in the medium, ct represents the cadmium concentration (μg/L) of the medium at time t, and C0 represents the initial cadmium concentration (μg/L) of the medium. BAF is commonly used to compare the loading of organisms to the pollution level in water. Cexposure, ccontrol, and Cwater represent the intracellular cadmium concentration (μg/g) of reniform worms and the cadmium concentration (μg/L) of deionized water in the experimental group, the control group, respectively.
In the embodiment of the invention, SPSS STATISTICS 20.0.0 (a piece of professional data statistical analysis software) is used for data analysis, and independent t test (INDEPENDENT T-test is used for comparing whether the mean values of two independent samples are significantly different or not) is used for testing the uniformity of the distribution and variance of the data. Differences between the treated and control groups were assessed using one-way analysis of variance (ANOVA) followed by a significant difference in Tukey (HSD) test. Dose-effect modeling was performed using MATLAB (a high-level computer language and environment), and histogram and line drawing was performed using Origin (a data analysis and mapping software) pairs.
B. the results of the examples of the present invention are described below:
(i) Reniform pest population density and response of metallothionein to cadmium
FIG. 1 is a graph showing the population density variation line of reniform worms during cadmium exposure time in an embodiment of the invention; it can be seen that the population density change results of the reniform worms within 96 hours of cadmium exposure show that the population density and the growth rate of the reniform worms are inhibited with the increase of the cadmium exposure concentration, and the population growth rate of the reniform worms is reduced to the minimum (0.03/h) when the cadmium exposure concentration reaches the maximum (24.85 mug/L).
Meanwhile, the dose-effect relation between the cadmium exposure concentration inside and outside the reniform worms and the population density is fitted based on MATLAB (Bio-Cd: Y=a+bX; ext-Cd: Y=a+bX+cX2), as shown in a table 1, which is a comparison table of a dose-effect regression equation of the cadmium exposure concentration inside and outside the reniform worms and the population density in the embodiment of the invention,
TABLE 1
In the regression equation of table 1, the dependent variable Y is the population density of reniform worms; the independent variable X in the row of Ext-Cd is the cadmium content (mug/L) of the kidney worm in-vitro culture solution, and the independent variable X in the row of Bio-Cd is the cadmium accumulation (mug/g) of the kidney worm; r 2 is a determination coefficient of the regression equation, is an important index for measuring the fitting degree of the regression equation, and the closer the value is to 1, the better the fitting effect of the regression equation is proved.
As shown in Table1, the results demonstrate that the inhibition effect of the reniform worms by cadmium is positively correlated with the exposure time and concentration, and the reniform worm population density is negatively correlated with the cadmium bioaccumulation.
The metallothionein is a key protein of organisms for resisting heavy metal toxicity, and as shown in table 2, the content of cadmium in the embodiment of the invention is influenced by the content of the metallothionein of the reniform worms, and the content of Cd-MTs of the metallothionein is continuously increased under 96 hours of cadmium gradient exposure;
TABLE 2
Fitting the dose effect results of MTs and the cadmium concentration inside and outside the reniform worms based on Allometric model (Y=a.Xb) in MATLAB, as shown in table 3, which is a comparison table of the dose effect regression equation of metallothionein and cadmium inside and outside the reniform worm cells in the embodiment of the invention;
TABLE 3 Table 3
In the regression equation of Table 3, the dependent variable Y is the content of Metallothionein (MTs) of the reniform worm; the independent variable X in a column of MTs/Bio-Cd is cadmium accumulation amount (mug/g) of the reniform worms; the independent variable X in the column of MTs/Ext-Cd is the cadmium content (μg/L) of the in vitro culture solution of the reniform worms.
As shown in Table 3, the Cd-MTs content increased with increasing cadmium concentration and increased with increasing cadmium exposure time.
In summary, with the increase of the external concentration of cadmium and the extension of the exposure time, the reniform worms can endure the cadmium by increasing the content of metallothionein, so that the adverse effect of cadmium stress on the population density and the growth rate of the reniform worms is relieved to a certain extent. These findings not only enhance the understanding of how organisms cope with heavy metal stress, but also provide important scientific basis for developing heavy metal pollution treatment strategies based on bioremediation technology.
(Ii) Reniform worm bioaccumulation effect on cadmium and pollution repair
As shown in Table 4, a comparison table of the changes of the cadmium concentration in the intracellular and extracellular culture solutions of the kidney worms in 96 hours in the embodiment of the invention shows that the cadmium concentration in the external solution of all experimental groups is obviously reduced based on the bioaccumulation effect of the kidney worms on the cadmium and the cadmium removal capability result in the environment, and the reduction of the cadmium in the external environment corresponds to the bioaccumulation effect of the kidney worms on the cadmium; as shown in Table 5, which is a comparative table of the efficiency of the reniform insects to environmental cadmium in the examples of the present invention, it is seen that the highest cadmium removal rate of the reniform insects at 96 hours reached 32.98.+ -. 0.74% (Table 5).
TABLE 4 Table 4
TABLE 5
It can be seen that the bioaccumulation of reniform worm cadmium is significantly positively correlated with the external environmental cadmium concentration, with differences in dose-time response between the low cadmium concentration group (L1, L2, L3) and the high cadmium concentration group (H1, H2, H3). In the low cadmium exposure group, the bioaccumulation of cadmium continues to increase over time.
In summary, reniform worms exhibit significant bioaccumulation and environmental removal capability with varying concentrations of cadmium exposure. Reniform worms, in particular, exhibit higher bioaccumulation rates and sustained accumulation capacities under low cadmium concentration exposure conditions. These findings provide important scientific basis for further developing and applying biotechnology-based heavy metal contaminated soil remediation strategies.
(Iii) Comprehensive evaluation model for environmental restoration predictability and effectiveness
Analysis of the mechanism of cadmium in the reniform pest treatment environment shows that the total cadmium removal efficiency is directly dependent on population density and treatment time. Based on this, the following mathematical formula (I) was constructed to design a pollution remediation strategy, and the time-dose-effect regression equation (Table 3) for MTs/Cd can predict the treatment outcome:
The variation of the cadmium concentration of the external environment in the time t;
The amount of accumulation of kidney worm cells on cadmium at time t (calculated according to MTs/Bio-Cd regression equation of Table 3)
Initial seed Density of K, reniform pest population
Population growth rate of reniform worms
T, experimental time (treatment time)
The response mechanism of the reniform worms to the cadmium provided by the embodiment of the invention opens up a new biotechnology method for repairing and evaluating the cadmium pollution of the water body. First, the reniform worm can be used to remove cadmium from contaminated water having an environmental cadmium concentration of less than 24.85 μg/L within 96 hours. And (3) adjusting the initial inoculation population density of the reniform worms through the mathematical formula (I), and further planning the environmental cadmium removal scheme. Meanwhile, the repair effect of the environmental cadmium can be evaluated through the population growth rate of the reniform worms. Therefore, the reniform worms can be rapidly propagated in water, and the dormant capsule of the reniform worms added into the cadmium-polluted soil can effectively slow down the migration of cadmium from the soil to the water body through bioaccumulation.
The result clearly shows the treatment function and potential of the reniform worms on cadmium, and through modeling and predictive analysis, the reniform worms can be deeply understood how to effectively remove cadmium from the environment, the formula (I) and the corresponding regression equation can be applied to design an environmental cadmium pollution repair strategy, the cadmium pollution repair result is predicted, and a solution is provided for solving the actual environmental problem based on a new thought.
The embodiment of the invention has the following beneficial effects:
(1) Bioaccumulation effects and environmental removal capabilities of reniform worms under different concentrations of cadmium exposure are disclosed and utilized. Reniform worms exhibit higher bioaccumulation rates and sustained accumulation capacities with low cadmium exposure. These unique properties make kidney-shaped insects an important tool in developing and applying biotechnology-based heavy metal contaminated water-soil remediation strategies;
(2) Reniform worms can tolerate cadmium by increasing the content of metallothionein, thereby relieving the adverse effect of cadmium stress. The discovery of the mechanism provides an effective means for a heavy metal pollution treatment strategy based on a bioremediation technology;
(3) The function and the potential of the reniform worms for treating cadmium are clearly verified, a predictive mathematical model formula is constructed through a corresponding regression equation, and a novel method is provided for solving the problem of cadmium pollution environment.
The foregoing is a further detailed description of the invention in connection with the preferred embodiments, and it is not intended that the invention be limited to the specific embodiments described. It will be apparent to those skilled in the art that several equivalent substitutions and obvious modifications can be made without departing from the spirit of the invention, and the same should be considered to be within the scope of the invention.
Claims (9)
1. A method for cadmium pollution remediation and effect assessment, comprising:
inoculating a kidney worm in a cadmium polluted environment, and treating cadmium in the environment by utilizing the kidney worm to repair cadmium pollution; recording the initial inoculation density of the reniform worm population, and measuring the population growth rate of the reniform worm, the bioaccumulation of the reniform worm cells to cadmium and the metallothionein content of the reniform worm along with the treatment time;
The repair results of cadmium contamination using reniform worms were predicted and evaluated according to the following formula:
；
Wherein, For initial inoculation density of the reniform pest population,/>Is the population growth rate of reniform worms/(In order to achieve a processing time, the processing time,For/>Time accumulation of reniform cells on cadmium,/>For/>The change amount of the cadmium concentration of the internal and external environments of the time reniform worms; wherein/>And calculating according to a dose effect regression equation of the cadmium accumulation amount and the metallothionein content of the reniform worms corresponding to the treatment time.
2. The method for cadmium pollution remediation and effect assessment of claim 1,And when the time is 24 hours, 48 hours, 72 hours and 96 hours, the dose effect regression equation of the cadmium accumulation amount and the metallothionein content of the corresponding reniform worm cells is as follows:
24 h： Y=4.69X0.16, R2=0.86
48 h： Y=4.29X0.18, R2=0.83
72 h： Y=3.82X0.17, R2=0.91
96 h： Y=3.33X0.15, R2=0.83
Wherein Y represents the dependent variable of the regression equation, X represents the independent variable of the regression equation, and R 2 is the coefficient of determination of the regression equation.
3. A method of cadmium pollution remediation comprising inoculating a kidney worm in a cadmium contaminated environment, treating cadmium in the environment with the kidney worm to remediate cadmium pollution; wherein, based on the planned cadmium removal target, the initial inoculation density of the reniform worm population is adjusted according to the following formula:
；
Wherein, For initial inoculation density of the reniform pest population,/>Is the population growth rate of reniform worms/(In order to achieve a processing time, the processing time,For/>Time accumulation of reniform cells on cadmium,/>For/>The change amount of the cadmium concentration of the internal and external environments of the time reniform worms; wherein/>And calculating according to a dose effect regression equation of the cadmium accumulation amount and the metallothionein content of the reniform worms corresponding to the treatment time.
4. The method for repairing cadmium pollution according to claim 3,And when the time is 24 hours, 48 hours, 72 hours and 96 hours, the dose effect regression equation of the cadmium accumulation amount and the metallothionein content of the corresponding reniform worm cells is as follows:
24 h： Y=4.69X0.16, R2=0.86
48 h： Y=4.29X0.18, R2=0.83
72 h： Y=3.82X0.17, R2=0.91
96 h： Y=3.33X0.15, R2=0.83
Wherein Y represents the dependent variable of the regression equation, X represents the independent variable of the regression equation, and R 2 is the coefficient of determination of the regression equation.
5. The method for remediation of cadmium pollution of claim 3 or 4 wherein cadmium is removed from the contaminated water having an environmental cadmium concentration of less than 24.85 μg/L in 96 hours.
6. The method for cadmium pollution remediation of claim 3 or 4, further comprising: the method for cadmium pollution repair and effect evaluation according to claim 1 or 2 is used for predicting and evaluating the effect of cadmium repair on the environment.
7. The method for cadmium pollution remediation of claim 3 or 4, further comprising: when the reniform worm cadmium is exposed for a preset time, the population density of the reniform worm is measured, and the environmental cadmium concentration is determined according to a dose effect regression equation of the environmental cadmium concentration and the reniform worm population density exposed for the preset time.
8. The method of cadmium pollution remediation of claim 7 wherein the corresponding dose effect regression equations for the environmental cadmium concentration and the reniform worm population density at the predetermined times 24h, 48h, 72h, 96h are as follows:
24 h： Y=0.84X2-58.52X+1579.54, R2=0.98
48 h： Y=2.14X2-120.48X+2267.45, R2=0.97
72 h： Y=16.68X2-618.70X+7150.25, R2=0.94
96 h： Y=23.72X2-1003.47X+13973.64, R2=0.88
Wherein Y represents the dependent variable of the regression equation, X represents the independent variable of the regression equation, and R 2 is the coefficient of determination of the regression equation.
9. The method of cadmium pollution remediation of claim 3 or 4 wherein inoculating the reniform worm in a cadmium-contaminated environment comprises adding a dormant capsule of reniform worm to cadmium-contaminated soil.
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| CN114933969A (en) * | 2022-06-09 | 2022-08-23 | 中国海洋大学 | Ciliate protozoan with plant seed germination and seedling growth promoting effect |
| CN116769869A (en) * | 2023-08-18 | 2023-09-19 | 哈尔滨工业大学(深圳)(哈尔滨工业大学深圳科技创新研究院) | Method for detecting sulfamethoxazole environmental concentration and environmental risk |
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| CN101586148A (en) * | 2009-06-26 | 2009-11-25 | 河北大学 | Method for degrading crude oil with protozoan infusorian |
| CN114933969A (en) * | 2022-06-09 | 2022-08-23 | 中国海洋大学 | Ciliate protozoan with plant seed germination and seedling growth promoting effect |
| CN116769869A (en) * | 2023-08-18 | 2023-09-19 | 哈尔滨工业大学(深圳)(哈尔滨工业大学深圳科技创新研究院) | Method for detecting sulfamethoxazole environmental concentration and environmental risk |
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