CN117946839A - Biological cell wall pretreatment crushing and filtering system - Google Patents

Biological cell wall pretreatment crushing and filtering system Download PDF

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Publication number
CN117946839A
CN117946839A CN202410136575.XA CN202410136575A CN117946839A CN 117946839 A CN117946839 A CN 117946839A CN 202410136575 A CN202410136575 A CN 202410136575A CN 117946839 A CN117946839 A CN 117946839A
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Prior art keywords
grinding
frame
wall
tooth
fixedly connected
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Inventor
罗丽华
李岗
张倩
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Kunming Bin'an Biotechnology Co ltd
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Kunming Bin'an Biotechnology Co ltd
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Priority to CN202410136575.XA priority Critical patent/CN117946839A/en
Publication of CN117946839A publication Critical patent/CN117946839A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B02CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02CCRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C13/00Disintegrating by mills having rotary beater elements ; Hammer mills
    • B02C13/14Disintegrating by mills having rotary beater elements ; Hammer mills with vertical rotor shaft, e.g. combined with sifting devices
    • B02C13/18Disintegrating by mills having rotary beater elements ; Hammer mills with vertical rotor shaft, e.g. combined with sifting devices with beaters rigidly connected to the rotor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B02CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02CCRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C13/00Disintegrating by mills having rotary beater elements ; Hammer mills
    • B02C13/20Disintegrating by mills having rotary beater elements ; Hammer mills with two or more co-operating rotors
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B02CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02CCRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C13/00Disintegrating by mills having rotary beater elements ; Hammer mills
    • B02C13/26Details
    • B02C13/28Shape or construction of beater elements
    • B02C13/2804Shape or construction of beater elements the beater elements being rigidly connected to the rotor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B02CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02CCRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C13/00Disintegrating by mills having rotary beater elements ; Hammer mills
    • B02C13/26Details
    • B02C13/282Shape or inner surface of mill-housings
    • B02C13/284Built-in screens
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B02CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02CCRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C13/00Disintegrating by mills having rotary beater elements ; Hammer mills
    • B02C13/26Details
    • B02C13/286Feeding or discharge
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B02CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
    • B02CCRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
    • B02C13/00Disintegrating by mills having rotary beater elements ; Hammer mills
    • B02C13/26Details
    • B02C13/30Driving mechanisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00Means for pre-treatment of biological substances
    • C12M45/02Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Polymers & Plastics (AREA)
  • Animal Husbandry (AREA)
  • Physiology (AREA)
  • Mechanical Engineering (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the technical field of yeast processing, in particular to a biological cell wall pretreatment crushing and filtering system. The invention can grind the waste beer yeast more fully, effectively improves the crushing effect of cell walls and reduces waste. The invention comprises a flow guiding frame, a supporting frame, an electric guide rail, an electric sliding block and the like; the two sides of the flow guiding frame are fixedly connected with supporting frames, the tops of the two supporting frames are fixedly connected with electric guide rails, and the two electric guide rails are connected with electric sliding blocks in a sliding mode. According to the invention, the two grinding sheets rotate and roll the brewing yeast cells in the grinding half frame to crush the cell walls of the yeast cells, so that substances in the yeast cells are released and dissolved in the solution, the first broken teeth rotate along the gear ring to drive the grinding sheets to rotate, and the two grinding sheets can sufficiently grind the yeast cells to sufficiently crush the cell walls of the yeast cells, so that a better wall breaking effect is achieved.

Description

Biological cell wall pretreatment crushing and filtering system
Technical Field
The invention relates to the technical field of cell wall crushing processing, in particular to a biological cell wall pretreatment crushing and filtering system.
Background
In biological applications, there are cases where it is necessary to destroy the cell wall in order to release the intracellular material or to perform further experimental procedures. For example: preparation of protoplasts: where protoplasts refer to the liquid fraction of the cytoplasm after rupture of the cytoplasmic membrane and cell wall.
It has been found that during brewing, spent yeast cells undergo a process of disruption and lysis, releasing the cell contents into the liquid to form protoplasts. Further studies have found that approximately 100t spent yeast is produced per 10000t of beer produced during beer brewing. The waste beer yeast contains rich protein, ribonucleic acid, vitamin B group, lipid, polysaccharide, enzyme and other nutritious components and has several physiological functions, so that it may be used in producing feed. However, since yeast has relatively firm cell walls, and substances in cells are not easily released, they cannot be well absorbed and utilized by animals as a feed, and therefore, a pretreatment process for disrupting the cell walls of spent beer yeast is required before the spent beer yeast is produced into a feed.
Researchers find that at present, common equipment can use grinding broken wall device to grind the yeast mud of beer waste yeast, then carry out filtrate treatment with broken cell solution to eliminate the residual material, but most grinding device breakage rate is lower, and the crushing effect is not good, can't fully break the cell wall of yeast, leads to the material in the yeast not fully released, and the yeast mud is very easy to adhere in the section of thick bamboo wall of device when empting moreover, is difficult for digging out, can cause the waste.
Disclosure of Invention
In order to solve the technical problems, the invention provides a biological cell wall pretreatment crushing and filtering system, which can grind waste beer yeast more fully, effectively improve the crushing effect of cell walls and reduce waste.
The invention provides a biological cell wall pretreatment crushing and filtering system, which comprises a flow guiding frame, supporting frames, electric guide rails, electric sliding blocks, a grinding and crushing mechanism and a liquid discharging mechanism, wherein the supporting frames are fixedly connected to two sides of the flow guiding frame, the electric guide rails are fixedly connected to the tops of the two supporting frames, the electric sliding blocks are connected to the two electric guide rails in a sliding mode, the grinding and crushing mechanism is arranged between the two electric sliding blocks, and the liquid discharging mechanism is arranged on the grinding and crushing mechanism;
the grinding and crushing mechanism comprises a supporting plate, a motor, a threaded long rod frame, a grinding sheet, first broken wall teeth and a gear ring;
A supporting plate is fixedly connected between the two electric sliding blocks, the motor is fixedly connected to the upper part of the supporting plate, the threaded long rod frame is fixedly connected to an output shaft of the motor, two grinding sheets are rotatably connected to the lower part of the threaded long rod frame, and first broken wall teeth are fixedly connected to the two grinding sheets; the gear ring is fixedly connected to the lower part of the supporting plate, the two first wall breaking teeth are positioned in the gear ring, and the two first wall breaking teeth are meshed with the gear ring;
The grinding and crushing mechanism further comprises an inner tooth and outer tooth grinding disc and a rotating shaft; the grinding sheet is rotationally connected to the lower part of the threaded long rod frame through a rotating shaft; the rotating shaft is of a sleeve structure provided with an inner wall cavity, and a grinding disc driving motor is fixedly arranged at the top of the inner wall cavity; the inner and outer teeth grinding sheets are arranged below the grinding sheets; the inner and outer tooth twisting abrasive sheets are arranged on the inner wall of the rotating shaft through the smooth bearing, and the twisting abrasive sheet driving motor extends out of the bottom of the rotating shaft through the output shaft and is connected with the inner and outer tooth twisting abrasive sheets in a key way;
The grinding sheets in the grinding and crushing mechanism are used for stirring the yeast cell sludge to form vortex with large diameter, and the inner and outer teeth grinding sheets in the grinding and crushing mechanism are used for stirring the yeast cell sludge to form vortex with smaller relative diameter.
As a further preferable scheme, the outer edge of the inner and outer tooth grinding sheets in the grinding and crushing mechanism is provided with a plurality of obliquely arranged tooth sheets, and each tooth sheet is sharpened to form a cutter tooth structure, namely a second broken wall tooth; meanwhile, an inner through hole is formed in the center of the inner and outer tooth grinding piece, a circle of obliquely arranged tooth pieces are arranged on the edge of the inner wall of the inner through hole, and each tooth piece is edged to form a cutter tooth structure, namely a third broken wall tooth;
A bearing step is arranged above the surface between the inner through hole and the outer edge of the inner and outer tooth grinding piece; the bearing step is arranged on the inner wall of the rotating shaft through a smooth bearing.
As a further preferable mode, the outer diameter dimension of the grinding sheet is larger than the outer diameter dimension of the inner and outer teeth grinding sheet, and the diameter dimension of the inner through hole in the inner and outer teeth grinding sheet is smaller than the outer diameter dimension of the rotating shaft.
As a further preferable scheme, the flow guiding frame is provided with a liquid outlet.
As a further preferred scheme, the tapping mechanism comprises a bent pressing rod, a rack long rod, a reset spring, a grinding half frame, a gear III, a torsion spring I and a cell filter membrane, wherein the bent pressing rod is connected to a supporting plate in a sliding mode, the bent pressing rod is connected with a threaded long rod frame through threads, two lower portions of electric guide rails are connected with the rack long rod in a sliding mode, the bent pressing rod is located above the two rack long rods, the reset spring is connected between the rack long rod and the electric guide rails, the two support frames are connected with the grinding half frame in a rotating mode, the two grinding half frames are in contact with each other, the two grinding half frames are located above the guide frame, the gear III is fixedly connected to the grinding half frames, the rack long rod is meshed with the gear III, the torsion spring I is connected between the grinding half frames and the support frames, and the cell filter membrane is fixedly connected in the guide frame.
As a further preferred scheme, the novel grinding machine further comprises a liquid guide mechanism, wherein the liquid guide mechanism is arranged on the threaded long rod frame, the liquid guide mechanism comprises a supporting right-angle rod, rotating wheels, a lantern ring and a spiral plate, the supporting right-angle rod is fixedly connected to the bottom of the threaded long rod frame, the supporting right-angle rod is positioned between two grinding sheets, both sides of the supporting right-angle rod are rotatably connected with the rotating wheels, both sides of the supporting right-angle rod are rotatably connected with lantern rings, the lantern rings are fixedly connected with the rotating wheels, and the spiral plates are fixedly connected to the outer walls of the lantern rings.
As a further preferred scheme, the novel hammer is characterized by further comprising a vibration mechanism, wherein the two support frames are provided with the vibration mechanism, the vibration mechanism comprises a hammer head rod, a torsion spring II, a sawtooth plate and a mandril, the two support frames are connected with the hammer head rod in a rotating mode, the hammer head rod is positioned below the grinding half frame, the torsion spring II is connected between the hammer head rod and the support frames, the two rack long rods are fixedly connected with the sawtooth plate, the two hammer head rods are connected with the mandril through hinges, and the sawtooth plate is positioned above the mandril.
As a further preferable scheme, the polishing device further comprises a supporting bent rod and a triangular scraper, wherein two supporting bent rods are fixedly connected to the supporting right-angle rod, the triangular scraper is fixedly connected to the two supporting bent rods, and the triangular scraper and the polishing sheet are located on the same horizontal line.
As a further preferable scheme, the electric grinding machine further comprises a gauze frame, wherein the gauze frame is fixedly connected between the lower parts of the two electric guide rails and is positioned in the two grinding half frames.
The invention has the following advantages:
1. According to the invention, the two electric sliding blocks are driven to move downwards through the electric guide rail, so that the first threaded long rod frame and the grinding sheet move downwards, the grinding sheet is contacted with the inner side of the grinding half frame, then the motor drives the threaded long rod frame to rotate, so that the two grinding sheets rotate, and the brewing yeast cells in the grinding half frame are rolled, the cell walls of the yeast cells are crushed, substances in the yeast cells are released and dissolved in a solution, the first broken wall teeth rotate along the gear ring to drive the grinding sheets to rotate, the two grinding sheets can sufficiently grind the yeast cells, and then the cell walls of the yeast cells are sufficiently crushed, so that a better broken wall effect is achieved.
2. According to the invention, the screw long rod frame moves downwards to drive the supporting right-angle rod to move downwards, so that the lantern ring and the screw plate move downwards together and are in contact with the bottom of the inner side of the grinding half frame, meanwhile, the screw long rod frame rotates to drive the supporting right-angle rod to rotate, so that the rotating wheel rotates and can rotate along the grinding half frame to drive the lantern ring and the screw plate to rotate, the two screw plates automatically rotate to guide the yeast cell solution in the middle of the two grinding half frames to the periphery, and the yeast cells concentrated in the middle of the two grinding half frames are spread out, so that the yeast cells can be ground more fully when the two grinding plates rotate, the cell walls of the yeast cells are broken more thoroughly, substances in the yeast cells are released more fully, and the cell wall breaking effect of the yeast cells is effectively improved.
3. According to the invention, the rack long rod moves downwards and drives the serration plate to move downwards, the serration plate moves downwards and pushes the ejector rod to swing downwards, the ejector rod swings downwards and drives the hammer head rod to swing downwards, the torsion spring II is twisted, the serration plate moves downwards and does not push the ejector rod, the torsion spring II resets and drives the hammer head rod and the ejector rod to swing upwards together rapidly, at the moment, the two grinding half frames also swing downwards, the hammer head rod swings upwards rapidly and can beat the bottom of the grinding half frame, the serration plate continues to move downwards and pushes the ejector rod to swing downwards again, and the steps are repeated, so that the bottom of the grinding half frame is continuously knocked, residual yeast cells in the grinding half frame are continuously vibrated, and a quantity of residual yeast cells adhered in the grinding half frame flow into the flow guiding frame along with a solution, so that waste of yeast mud is avoided.
Drawings
FIG. 1 is a schematic perspective view of a biological cell wall pretreatment crushing filtration system without an inner and outer teeth abrasive disc;
FIG. 2 is a schematic view of a part of the structure of FIG. 1;
FIG. 3 is a schematic perspective view of a biological cell wall pretreatment crushing filtration system provided by the invention after an inner and outer tooth grinding disc is arranged;
FIG. 4 is a partial schematic view of the structure of FIG. 3;
FIG. 5 is a schematic perspective view of an explosion assembly between an abrasive sheet, a rotating shaft, an inner and outer teeth abrasive sheet and an abrasive sheet driving motor in a biological cell wall pretreatment crushing and filtering system;
FIG. 6 is a schematic diagram of the overall structure of an inner and outer teeth-twisted abrasive disc in a biological cell wall pretreatment crushing and filtering system provided by the invention;
FIG. 7 is a schematic view of a part of an inner and outer teeth-twisted abrasive disc in a biological cell wall pretreatment crushing and filtering system according to the present invention;
FIG. 8 is an enlarged perspective view of the structure of FIG. 1A according to the present invention;
FIG. 9 is a schematic perspective view of a liquid guiding mechanism according to the present invention;
FIG. 10 is an enlarged perspective view of the structure of the B in FIG. 1 according to the present invention;
FIG. 11 is a schematic view of a split perspective structure of a grinding half frame according to the present invention;
FIG. 12 is a schematic view of a partial perspective structure of the present invention;
FIG. 13 is a schematic perspective view of a flow guiding frame according to the present invention;
Wherein: 1-flow guiding frame, 2-supporting frame, 31-electric guide rail, 32-electric sliding block, 41-supporting plate, 42-motor, 43-screw long rod frame, 44-grinding sheet, 45-first broken tooth, 46-gear ring, inner and outer teeth grinding sheet 47, second broken tooth 471, third broken tooth 472, bearing step 473, rotating shaft 48, grinding sheet driving motor 49, 51-bent press rod, 52-rack long rod, 53-return spring, 54-grinding half frame, 55-gear three, 56-torsion spring one, 57-cell filter membrane, 61-supporting right angle rod, 62-rotating wheel, 63-lantern ring, 64-spiral plate, 71-hammer head rod, 72-torsion spring two, 73-saw tooth plate, 74-ejector rod, 81-supporting bent rod, 82-triangular scraper and 9-gauze frame.
Detailed Description
Standard parts used in the invention can be purchased from the market, special-shaped parts can be customized according to the description of the specification and the drawings, and the specific connection modes of the parts adopt conventional means such as mature bolts, rivets, welding, pasting and the like in the prior art, and the detailed description is omitted.
Example 1:
As shown in fig. 1 to 13, embodiment 1 of the present invention provides a biological cell wall pretreatment crushing and filtering system, which comprises a flow guiding frame 1, a supporting frame 2, an electric guide rail 31, an electric sliding block 32, a grinding and crushing mechanism, a liquid discharging mechanism and other mechanisms;
The two sides of the flow guiding frame 1 are connected with a supporting frame 2 through bolts, the tops of the two supporting frames 2 are connected with an electric guide rail 31 through bolts, the two electric guide rails 31 are connected with electric sliding blocks 32 in a sliding mode, a grinding and crushing mechanism is arranged between the two electric sliding blocks 32, and the liquid discharging mechanism is arranged on the grinding and crushing mechanism. A liquid outlet is formed in the flow guiding frame 1.
The biological cell wall pretreatment crushing and filtering system provided by the embodiment of the invention adopts a semi-open structural design as a main structure, and the semi-open structural design of the grinding semi-frame is beneficial to the installation and maintenance of each mechanism; meanwhile, air circulation is increased, so that continuous fermentation of broken cell mud caused by long-time airtight stirring is avoided;
The grinding and crushing mechanism comprises a supporting plate 41 (the structures of the supporting plate 41, the electric guide rail 31 and the like are mainly manufactured by aluminum profiles), a motor 42, a threaded long rod frame 43, a grinding sheet 44, first broken wall teeth 45 and a gear ring 46, wherein the supporting plate 41 is connected between the two electric sliding blocks 32 through bolts, the motor 42 is connected to the upper part of the supporting plate 41 through bolts, namely the main function of the supporting plate 41 is to support and fix the motor 42 (the motor 42 is preferably in a stepping motor structure mode, and meanwhile, the motor 42 needs special explosion-proof and moisture-proof treatment); the long threaded rod frame 43 is fixedly connected to an output shaft of the motor 42 (the long threaded rod frame 43 and the motor are coaxially arranged, for example, coaxial rotation connection is realized through a spline, etc.), the long threaded rod frame 43 is vertically arranged, and two grinding sheets 44 (or grinding pieces) are rotatably connected to the lower part of the long threaded rod frame 43;
The outer edges of the two grinding sheets 44 are fixedly connected with first broken wall teeth 45, the gear ring 46 is connected to the lower portion of the supporting plate 41 through bolts, the two first broken wall teeth 45 are located in the gear ring 46, and the two first broken wall teeth 45 are meshed with the gear ring 46.
The liquid discharging mechanism comprises a bent compression bar 51, a rack long bar 52, a return spring 53, a grinding half frame 54, a third gear 55, a first torsion spring 56 and a cell filter membrane 57;
The bent compression rod 51 is slidably connected to the support plate 41, the bent compression rod 51 is in threaded connection with the threaded long rod frame 43, the lower parts of the two electric guide rails 31 are both slidably connected with the rack long rods 52, the bent compression rod 51 is located above the two rack long rods 52, a return spring 53 is connected between the rack long rods 52 and the electric guide rails 31, the two support frames 2 are both rotatably connected with a grinding half frame 54, and the two grinding half frames 54 are in contact; two grind half frame 54 all are located water conservancy diversion frame 1 top, two grind half frame 54 is last to be connected with the gear three 55 through the parallel key, rack stock 52 and gear three 55 meshing, be connected with torsion spring one 56 between grinding half frame 54 and the support frame 2, cell filter 57 fixed connection is in water conservancy diversion frame 1.
The main structure of the biological cell wall pretreatment crushing and filtering system is analyzed as follows:
In actual operation, the staff firstly pours the yeast slurry of the waste beer yeast into the two grinding half frames 54, so that the yeast slurry just covers the bottoms of the inner sides of the two grinding half frames 54, then starts the electric guide rail 31, and the electric guide rail 31 drives the two electric sliding blocks 32 to move downwards, so as to drive the supporting plate 41, the motor 42, the threaded long rod frame 43 and other component structures to move downwards together;
simultaneously, in the process of moving down the screw thread long rod frame 43, the screw thread long rod frame 43 can drive the grinding sheet 44, the first broken wall teeth 45 and the gear ring 46 to move down together, when the grinding sheet 44 moves down to be in contact with the inner side of the grinding half frame 54, the electric guide rail 31 is closed, and the motor 42 is started, an output shaft of the motor 42 (namely, a stepping motor) rotates to drive the screw thread long rod frame 43 to rotate, the screw thread long rod frame 43 rotates to drive the two grinding sheets 44 and the two first broken wall teeth 45 to rotate together, brewing yeast cells in the grinding half frame 54 can be crushed under the self-rotation stirring action of the two grinding sheets 44 and the inter-tooth friction action between the grinding sheets 44 and the gear ring 46 (therefore, the structural design has double wall breaking and double stirring functions, wherein the single stirring function is realized through the inter-tooth friction wall breaking function between the grinding sheets 44 and the gear ring 46, and the inner and outer broken wall breaking action of the inner and outer broken wall teeth 47 matched with the grinding sheets 44), the material in the yeast cell wall is crushed, the material in the yeast cell wall is released from the cell wall is fully crushed, and the first broken wall 45 rotates along the first broken wall 45 and the rotation effect of the gear ring 46, and the cell wall is fully ground, and the cell wall is fully rotated and the rotation and the cell wall is fully ground by the rotation of the first broken wall and the rotation wall and the gear wall is fully rotates.
However, in a further technical scheme, in order to enhance the wall breaking efficiency and the wall breaking efficiency of the grinding sheet, the grinding sheet actually adopts a structural design of a composite wall breaking mode; a composite wall-broken structure, namely an upper-mounted grinding sheet 44 and a lower-mounted inner and outer teeth-twisted grinding sheet 47, thereby forming a multi-tooth wall-broken structure; see example 2 for details regarding the structure of the inner and outer teeth wear plates;
Subsequently, the screw long rod frame 43 moves downwards and drives the bent compression rod 51 to move downwards, the screw long rod frame 43 rotates and drives the bent compression rod 51 to move downwards, the bent compression rod 51 moves downwards and contacts the rack long rods 52 to push the two rack long rods 52 to move downwards, the reset spring 53 is compressed, the rack long rods 52 move downwards and drive the three gears 55 to rotate, the three gears 55 rotate and drive the grinding half frame 54 to swing downwards, the torsion spring I56 is twisted, the two grinding half frames 54 swing downwards and pour the grinded yeast mud into the guide frame 1, the cell filter membrane 57 filters the yeast cell solution, residual residues of the yeast cell solution are filtered, filtrate is clarified, and the filtered filtrate is discharged from a liquid outlet of the guide frame 1.
Then, the operator adjusts the output shaft of the motor 42 to reversely rotate, so that the threaded long rod frame 43 reversely rotates and drives the bent compression rod 51 to upwardly move, the bent compression rod 51 upwardly moves to be separated from contact with the rack long rod 52, the reset spring 53 resets and drives the rack long rod 52 to upwardly move, the rack long rod 52 upwardly moves and drives the gear III 55 to reversely rotate, the gear III 55 reversely rotates and drives the grinding half frame 54 to upwardly swing, the torsion spring I56 resets, then the operator turns off the motor 42 and starts the electric guide rail 31, the electric guide rail 31 drives the two electric sliding blocks 32 to upwardly reset, the supporting plate 41, the motor 42 and the threaded long rod frame 43 are further driven to upwardly reset together, and the threaded long rod frame 43 upwardly resets and drives the grinding sheet 44, the inner and outer tooth grinding sheets 47, the first broken wall teeth 45, the gear ring 46 and the bent compression rod 51 to upwardly reset together.
Example 2:
On the basis of the embodiment 1, as shown in fig. 3-7, the embodiment 2 of the invention provides a biological cell wall pretreatment crushing and filtering system, which comprises a flow guiding frame 1, a supporting frame 2, an electric guide rail 31, an electric sliding block 32, a grinding and crushing mechanism, a liquid discharging mechanism and other mechanisms;
While also having other structural designs identical to those of embodiment 1, embodiment 2 also has a unique structural design, unlike embodiment 1;
I.e. the grinding and crushing mechanism further comprises inner and outer teeth grinding plates 47; the inner and outer teeth grinding sheets 47 are arranged below the grinding sheets 44, and the grinding sheets 44 are connected below the threaded long rod frame through the rotating shafts 48 (the rotating shafts 48 in the embodiment of the invention are actually rotatable sleeve structures); the inner and outer teeth grinding sheets 47 are mounted on the inner wall of the rotating shaft through the smooth bearing, and the inner and outer teeth grinding sheets 47 and the grinding sheets 44 realize coaxial rotation (but the rotation mode of "coaxial different speeds" in the embodiment of the invention means that the grinding and crushing mechanism adopts the "coaxial different speeds" wall breaking design concept for the first time);
In embodiment 2 of the present invention, there are at least two significant design structural features and technical advantages for the inner and outer teeth grinding sheets 47 in the grinding and crushing mechanism;
First aspect: the inner and outer teeth grinding disc 47 is a wall breaking disc, but the inner and outer teeth grinding disc 47 has an inner and outer wall breaking cutter tooth structure, that is, the edges of the inner and outer teeth grinding disc 47 are provided with a plurality of obliquely arranged tooth sheets, and each tooth sheet is edged to form a cutter tooth structure (that is, form a second wall breaking tooth 471); meanwhile, an inner through hole is arranged in the center of the inner wall of the inner through hole, a circle of obliquely arranged tooth sheets (a plurality of tooth sheets are also formed into a third broken wall tooth 472), and each tooth sheet is edged to form a cutter tooth structure; the wall breaking effect of the inner and outer tooth grinding sheets 47 can be obviously improved by adopting a cutter tooth structure inside and outside the machine;
Second aspect: the inner and outer teeth grinding sheets 47 and 44 realize coaxial rotation, but the two may generate relative rotation of 'coaxial different speeds', namely 'coaxial different speeds'; however, it should be noted that the "coaxial" is not strictly a same rotation axis, but a coaxial rotation, and the specific scheme is as follows:
In a specific structural design, a bearing step 473 is provided above the surface between the inner through hole and the outer edge of the inner and outer tooth wear plates 47; the bearing step is arranged on the inner wall of the rotating shaft 48 through a smooth bearing (namely a deep groove ball bearing), namely, the bearing step is arranged below the inner and outer tooth grinding disc 47 and is arranged on the inner wall of the rotating shaft 48 through the smooth bearing (the outer wall of the bearing step 473 is in interference fit with the inner ring of the smooth bearing to realize fastening installation, and small hammer striking assembly can be used in the installation process), but the relative rotation of the rotating shaft does not drive the inner and outer tooth grinding disc 47 to synchronously rotate; because the inner and outer teeth grinding sheets 47 are driven to rotate by separate motors (namely grinding sheet driving motors), different rotation speed outputs of the grinding sheet 44 can be realized as long as the output rotation speed of the grinding sheet driving motor is kept different from the rotation speed of the grinding sheet driving motor; the grinding disc driving motor is fixedly arranged at the top of the inner cavity of the rotating shaft 48, namely the rotating shaft 48 is actually designed into an inner wall cavity (namely the rotating shaft 48 is of a sleeve structure), and the top of the inner wall cavity of the rotating shaft 48 is fixedly connected with an independent driving motor in a hidden manner, namely the grinding disc driving motor;
Simultaneously, the output shaft of the grinding disc driving motor extends outwards and penetrates out of the rotating shaft 48 to extend downwards continuously to be connected with the inner tooth grinding disc 47 at the bottommost part in a key way; thus, strictly speaking, the motor output shaft of the abrasive sheet drive motor in which the inner and outer teeth abrasive sheets 47 are driven and the rotating shaft 48 in which the abrasive sheets 44 are driven are not exactly one shaft (one rotating shaft), but are in coaxial relationship (coaxial relationship), thereby forming a so-called "coaxial different-speed" abrasive structure;
generally, no special rotation speed design exists, the rotation speeds of the rotation shaft and the motor output shaft of the grinding disc driving motor are different for most of time, and the structural design is found in the further research process that because the inner and outer tooth grinding discs 47 and the grinding discs 44 rotate at different rotation speeds, the wall breaking effect is further improved, generally, the grinding discs 44 positioned at the upper position have stirring and wall breaking effects, so that the cell wall in the grinding half frame is broken as much as possible, a stirring vortex with larger diameter is formed from the top of the grinding half frame, however, if the inner and outer tooth grinding discs 47 also rotate at the same rotation speed completely and coaxially, the cell wall breaking effect is poor, but the output of the inner and outer tooth grinding discs 47 with different rotation speeds can cause vortex turbulence or smaller vortex to be formed at the lower part of the grinding half frame, and the vortex is rubbed with each other, so that the flowability of the yeast mud is enhanced, and the wall breaking effect of the cell wall is also enhanced.
Because the internal and external teeth grinding piece is arranged below the grinding piece, the movement distance can be limited when the screw thread long rod frame moves downwards, and the mesh cloth of the half frame is prevented from being broken by the second wall breaking teeth 471 of the internal and external teeth grinding piece, so that the two mesh cloths are in conflict.
Example 3:
on the basis of the embodiment 1, as shown in fig. 1-13, the embodiment 3 of the invention provides a biological cell wall pretreatment crushing and filtering system, which further comprises a liquid guide mechanism, wherein the liquid guide mechanism is arranged on a threaded long rod frame 43 and comprises a supporting right angle rod 61, a rotating wheel 62, a lantern ring 63 and a spiral plate 64;
The support right angle pole 61 is connected at the bottom of screw thread long pole frame 43 through the bolt, the support right angle pole 61 is located between two abrasive sheets 44, the both sides of support right angle pole 61 all are connected with the rotation wheel 62 in a rotating way, the both sides of support right angle pole 61 all are connected with the lantern ring 63 in a rotating way, lantern ring 63 and rotation wheel 62 fixed connection, two all fixedly connected with screw plate 64 on the outer wall of lantern ring 63.
As can be seen from an analysis of the specific structure of the liquid guiding mechanism, the long threaded rod frame 43 moves downward and drives the supporting right angle rod 61 to move downward, the supporting right angle rod 61 moves downward and drives the rotating wheel 62, the collar 63 and the spiral plate 64 to move downward together, the rotating wheel 62 and the spiral plate 64 move downward and contact with the bottom of the inner side of the grinding half frame 54, and the long threaded rod frame 43 rotates and drives the supporting right angle rod 61 to rotate;
The rotation of the supporting right-angle rod 61 drives the rotating wheel 62, the lantern ring 63 and the spiral plate 64 to rotate together, the rotating wheel 62 rotates and rotates along the bottom of the inner side of the grinding half frame 54, and the rotating wheel 62 rotates and drives the lantern ring 63 and the spiral plate 64 to rotate together; since the rotation of the two grinding sheets 44 can squeeze the yeast cells to the middle of the two grinding half frames 54, the two spiral plates 64 automatically guide the yeast cell solution in the middle of the two grinding half frames 54 to the surroundings, so that the yeast cells concentrated in the middle of the two grinding half frames 54 are spread out, the two grinding sheets 44 can grind the yeast cells more fully when rotating, the cell walls of the yeast cells are crushed more thoroughly, and substances in the yeast cells are released more fully, so that the cell wall crushing effect of the yeast cells is effectively improved;
At the same time, the upward reset of the long threaded rod frame 43 drives the supporting right angle rod 61 to upward reset, the upward reset of the supporting right angle rod 61 drives the rotating wheel 62, the lantern ring 63 and the spiral plate 64 to upward reset together, and the upward reset of the rotating wheel 62 and the spiral plate 64 is separated from contact with the grinding half frame 54.
Example 4:
On the basis of the embodiment 2, as shown in fig. 1-10, the vibration device is further included, and the vibration devices are arranged on both the supporting frames 2;
The vibration mechanism comprises a hammer head rod 71, a torsion spring II 72, a sawtooth plate 73 and a mandril 74; the two support frames 2 are rotatably connected with hammer head rods 71, the hammer head rods 71 are located below the grinding half frame 54, torsion springs II 72 are connected between the hammer head rods 71 and the support frames 2, the lower portions of the two rack long rods 52 are fixedly connected with sawtooth plates 73, the two hammer head rods 71 are connected with ejector rods 74 through hinges, and the sawtooth plates 73 are located above the ejector rods 74.
As can be seen from analyzing the specific structure and working principle of the vibration mechanism, the rack long rod 52 moves downwards and drives the serration plate 73 to move downwards, the serration plate 73 moves downwards and drives the ejector rod 74 to swing downwards, the ejector rod 74 swings downwards and drives the hammer head rod 71 to swing downwards, the torsion spring II 72 is twisted, the serration plate 73 moves downwards and does not push the ejector rod 74 any more, the torsion spring II 72 resets and drives the hammer head rod 71 and the ejector rod 74 to swing upwards together, at the moment, the two grinding half frames 54 also swing downwards, the hammer head rod 71 swings upwards rapidly and strikes the bottom of the grinding half frame 54, and the serration plate 73 continues to move downwards and pushes the ejector rod 74 to swing downwards again;
Repeatedly, the bottom of the grinding half frame 54 is continuously knocked, and residual yeast cells in the grinding half frame 54 are continuously vibrated, so that some residual yeast cells adhered in the grinding half frame 54 flow into the flow guiding frame 1 along with the solution, and waste of yeast mud is avoided; the rack bar 52 continuously pushes the ejector pin 74 to swing upward when moving upward, and is out of contact with the ejector pin 74.
Example 5:
On the basis of embodiment 4, as shown in fig. 1-12, the polishing device further comprises a supporting bent rod 81 and a triangular scraping plate 82, wherein two supporting bent rods 81 are connected to the supporting right-angle rod 61 through bolts, the triangular scraping plate 82 is connected to the two supporting bent rods 81 through bolts, and the triangular scraping plate 82 and the polishing sheet 44 are positioned on the same horizontal line.
The supporting right angle rod 61 moves downwards and drives the two supporting bent rods 81 and the triangular scraping plates 82 to move downwards together, the two triangular scraping plates 82 move downwards and contact the inner bottoms of the two half grinding frames 54, the supporting right angle rod 61 rotates and drives the two supporting bent rods 81 and the triangular scraping plates 82 to rotate together, the two triangular scraping plates 82 rotate and scrape yeast cells at the inner bottoms of the half grinding frames 54, the ground yeast cells are prevented from adhering to the inner bottoms of the half grinding frames 54, and further the grinding sheets 44 can grind the yeast cells better and pour the yeast cells better; the upward return of the support right angle bar 61 will drive the two support bent bars 81 and the triangular scraper 82 to return upward together.
Example 6:
On the basis of the embodiment 5, as shown in fig. 1-12, the electric grinding machine further comprises a gauze frame 9, wherein the gauze frame 9 is connected between the lower parts of the two electric guide rails 31 through bolts, and the gauze frame 9 is positioned in the two grinding half frames 54.
The specific structure of the gauze frame 9 was analyzed as follows: initially, the worker pours the yeast slurry into the gauze frame 9, then brings the two grinding sheets 44 into contact with the gauze frame 9, grinds the yeast slurry, and rotates the rotating wheel 62, the collar 63 and the spiral plate 64 into contact with the gauze frame 9 and rotates along with the gauze frame 9;
simultaneously, the triangular scraping plate 82 is also contacted with the gauze frame 9, yeast mud on the gauze frame 9 is scraped, the gauze frame 9 can filter the yeast mud, so that the grinded cell solution flows into the two grinding half frames 54, and the yeast mud can be left on the gauze frame 9, so that the yeast mud is subjected to more sufficient solid-liquid separation.
In summary, the biological cell wall pretreatment crushing and filtering system provided by the embodiment of the invention fully utilizes the characteristics of high viscosity and difficult crushing of residual yeast cells, adopts a specific structural design, utilizes the mechanisms such as a grinding and crushing mechanism and a liquid discharging mechanism of a composite structure, realizes a practical functional design with high efficiency and remarkable wall breaking effect, and enables some residual yeast cells adhered in the grinding half frame to flow into the flow guiding frame along with the solution by continuously vibrating the residual yeast cells in the grinding half frame, thereby avoiding waste of yeast mud and greatly improving the operation efficiency.
The foregoing has shown and described the basic principles and main features of the present invention and the advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (9)

1. A biological cell wall pretreatment disruption filtration system, characterized in that: comprises a flow guiding frame (1), a supporting frame (2), an electric guide rail (31), an electric sliding block (32), a grinding and crushing mechanism and a liquid discharging mechanism;
The two sides of the flow guiding frame (1) are fixedly connected with supporting frames (2), the tops of the two supporting frames (2) are fixedly connected with electric guide rails (31), and the two electric guide rails (31) are connected with electric sliding blocks (32) in a sliding mode;
A grinding and crushing mechanism is arranged between the two electric sliding blocks (32), and the liquid discharging mechanism is arranged on the grinding and crushing mechanism;
The grinding and crushing mechanism comprises a supporting plate (41), a motor (42), a threaded long rod frame (43), a grinding sheet (44), first broken wall teeth (45) and a gear ring (46);
A supporting plate (41) is fixedly connected between the two electric sliding blocks (32), the motor (42) is fixedly connected to the upper portion of the supporting plate (41), the threaded long rod frame (43) is fixedly connected to an output shaft of the motor (42), two grinding sheets (44) are rotatably connected to the lower portion of the threaded long rod frame (43), and first wall breaking teeth (45) are fixedly connected to the two grinding sheets (44); the gear ring (46) is fixedly connected to the lower part of the supporting plate (41), the two first wall breaking teeth (45) are positioned in the gear ring (46), and the two first wall breaking teeth (45) are meshed with the gear ring (46);
The grinding and crushing mechanism further comprises an inner tooth grinding disc (47) and an outer tooth grinding disc and a rotating shaft (48); the grinding sheet (44) is rotatably connected to the lower part of the threaded long rod frame (43) through a rotating shaft (48); the rotating shaft (48) is of a sleeve structure provided with an inner wall cavity, and a grinding disc driving motor (49) is fixedly arranged at the top of the inner wall cavity; the inner and outer teeth grinding sheets (47) are arranged below the grinding sheets (44); the inner and outer teeth grinding sheets (47) are arranged on the inner wall of the rotating shaft (48) through the smooth bearings, and the grinding sheet driving motor (49) extends out of the bottom of the rotating shaft (48) through an output shaft and is connected with the inner and outer teeth grinding sheets (47) in a key way;
The grinding sheet (44) in the grinding and crushing mechanism is used for stirring yeast cell sludge to form vortex with large diameter size, and the inner and outer tooth grinding sheets (47) in the grinding and crushing mechanism are used for stirring yeast cell sludge to form vortex with smaller relative diameter size.
2. A biological cell wall pretreatment disruption filtration system as claimed in claim 1 wherein: the outer edge of an inner and outer tooth grinding sheet (47) in the grinding and crushing mechanism is provided with a plurality of obliquely arranged tooth sheets, and each tooth sheet is edged to form a cutter tooth structure, namely a second broken wall tooth; meanwhile, an inner through hole is further formed in the center of the inner and outer tooth grinding piece (47), a circle of obliquely arranged tooth plates are arranged on the inner wall edge of the inner through hole, and each tooth plate is formed into a cutter tooth structure one by one through sharpening, namely a third broken wall tooth is formed;
a bearing step is arranged above the surface between the inner through hole and the outer edge of the inner and outer tooth grinding disc (47); the bearing step is mounted on the inner wall of the rotating shaft (48) through a smooth bearing.
3. A biological cell wall pretreatment disruption filtration system as claimed in claim 2 wherein: the outer diameter of the grinding sheet (44) is larger than that of the inner and outer teeth grinding sheets (47), and the diameter of the inner through hole in the inner and outer teeth grinding sheets (47) is smaller than that of the rotating shaft (48).
4. A biological cell wall pretreatment disruption filtration system as claimed in claim 3 wherein: a liquid outlet is formed in the flow guiding frame (1).
5. A biological cell wall pretreatment disruption filtration system according to claim 4, wherein: the liquid discharge mechanism comprises a bent compression bar (51), a rack long bar (52), a reset spring (53), a grinding half frame (54), a gear III (55), a torsion spring I (56) and a cell filter membrane (57);
The bent compression bar (51) is connected to the supporting plate (41) in a sliding mode, the bent compression bar (51) is connected with the threaded long bar frame (43) through threads, and the lower portions of the two electric guide rails (31) are connected with rack long bars (52) in a sliding mode;
the utility model discloses a cell filter, including curved depression bar (51), rack stock (52), electric guide (31) and cell filter membrane (57), curved depression bar (51) are located two rack stock (52) top, be connected with reset spring (53) between rack stock (52) and electric guide (31), two all rotate on support frame (2) and be connected with half grinding (54), two half grinding (54) contact, two half grinding (54) are located water conservancy diversion frame (1) top, two all fixedly connected with third gear (55) on half grinding (54), rack stock (52) and third gear (55) meshing, be connected with torsion spring (56) between half grinding (54) and support frame (2), cell filter membrane (57) fixed connection is in water conservancy diversion frame (1).
6. A biological cell wall pretreatment disruption filtration system as claimed in claim 5, wherein: still including liquid guiding mechanism, liquid guiding mechanism establishes on screw thread stock frame (43), liquid guiding mechanism is including supporting right angle pole (61), rotation wheel (62), lantern ring (63) and screw plate (64), support right angle pole (61) fixed connection in the bottom of screw thread stock frame (43), support right angle pole (61) are located between two abrasive sheet (44), the both sides of support right angle pole (61) are all rotationally connected with rotation wheel (62), the both sides of support right angle pole (61) are all rotationally connected with lantern ring (63), lantern ring (63) and rotation wheel (62) fixed connection, two all fixedly connected with screw plate (64) on the outer wall of lantern ring (63).
7. A biological cell wall pretreatment disruption filtration system as claimed in claim 6, wherein: still including vibration mechanism, two all be equipped with vibration mechanism on support frame (2), vibration mechanism is including hammer head pole (71), torsion spring two (72), serration board (73) and ejector pin (74), two all be connected with hammer head pole (71) on support frame (2) in the rotation, hammer head pole (71) are located and grind half frame (54) below, be connected with torsion spring two (72) between hammer head pole (71) and support frame (2), two rack stock (52) lower part all fixedly connected with serration board (73), two all be connected with ejector pin (74) through the hinge on hammer head pole (71), serration board (73) are located ejector pin (74) top.
8. A biological cell wall pretreatment disruption filtration system as claimed in claim 7, wherein: still including supporting bent rod (81) and triangle scraper blade (82), support fixedly connected with two on right angle bar (61) and support bent rod (81), two all fixedly connected with triangle scraper blade (82) on supporting bent rod (81), triangle scraper blade (82) are located same horizontal line with abrasive disc (44).
9. A biological cell wall pretreatment disruption filtration system as claimed in claim 8, wherein: the electric grinding machine is characterized by further comprising a gauze frame (9), wherein the gauze frame (9) is fixedly connected between the lower parts of the two electric guide rails (31), and the gauze frame (9) is positioned in the two grinding half frames (54).
CN202410136575.XA 2024-01-31 2024-01-31 Biological cell wall pretreatment crushing and filtering system Pending CN117946839A (en)

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CN117065856A (en) * 2023-08-21 2023-11-17 津市市大新颜料有限公司 Crushing and grinding equipment for pigment production
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CN209450374U (en) * 2018-10-17 2019-10-01 广东美的白色家电技术创新中心有限公司 Blade for blender and the blade assembly with it
JP6704497B1 (en) * 2019-07-05 2020-06-03 南京▲沢▼▲朗▼生物科技有限公司 Blender for extracting nutrients in plants
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