CN117907197A - Blood cell analyzer and blood cell analysis method - Google Patents
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- CN117907197A CN117907197A CN202311851369.9A CN202311851369A CN117907197A CN 117907197 A CN117907197 A CN 117907197A CN 202311851369 A CN202311851369 A CN 202311851369A CN 117907197 A CN117907197 A CN 117907197A
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Abstract
Embodiments of the present application relate to a blood cell analyzer and a blood cell analysis method. Firstly, sucking a blood sample to be tested; mixing a part of a blood sample to be measured, a hemolytic agent and a first fluorescent dye to prepare a first measurement sample, and making particles in the first measurement sample pass through an optical detection area irradiated by light one by one to obtain first optical information generated by the particles in the first measurement sample after being irradiated by light; mixing another portion of the blood sample to be measured, a diluent, and a second fluorescent dye to prepare a second measurement sample, and passing particles in the second measurement sample one by one through the optical detection zone irradiated with light to obtain second optical information generated by the particles in the second measurement sample after being irradiated with light; and acquiring a nucleated red blood cell analysis result of the blood sample to be tested by combining the first optical information and the second optical information. The embodiment of the application can improve the identification accuracy of nucleated red blood cells.
Description
Technical Field
The present application relates to the field of blood analysis, and in particular to a blood cell analyzer and a blood cell analysis method.
Background
The blood routine examination is a clinical basic examination item, and the detection results generally comprise the results of a leucocyte count value, a red blood cell count value, a platelet count value, a hemoglobin concentration, a reticulocyte count value and the like, and also comprise white blood cells, red blood cells, platelets and reticulocyte scatter diagrams or histograms obtained in the detection process so as to assist doctors in making clinical diagnoses.
The existing blood cell analyzer is based on fluorescent staining technology for performing optical measurement of white blood cells and optical measurement of reticulocytes (or platelet optical measurement), wherein the count value and classification of white blood cells including count value and classification of neutrophils, lymphocytes, monocytes, eosinophils and basophils are obtained in the optical measurement of white blood cells, and parameters such as red blood cell count value, platelet count value, reticulocyte count value and the like are obtained in the optical measurement of reticulocytes.
Mature erythrocytes are coreless, and the components are protein and iron, with the function of transporting oxygen. Cell growth is not possible without nuclei, nucleated erythrocytes are immature erythrocytes. Nucleated erythrocytes account for a small proportion and are rarely seen in normal peripheral blood. However, nucleated erythrocytosis occurs in cases of proliferative anaemia, erythroleukemia, pernicious anaemia, tumors, and the like.
In the existing blood cell analyzer, in order to accurately identify nucleated red blood cells in blood, a nucleated red blood cell detection channel is generally exclusively provided, but this leads to an increase in detection cost and a decrease in detection efficiency.
For this reason, the prior art has also proposed that the identification of nucleated erythrocytes is performed in the existing optical measurement of leukocytes or the optical measurement of reticulocytes, but the result of the identification of nucleated erythrocytes thus obtained is not ideal.
Disclosure of Invention
Against this background, the task of the present application is to accurately identify nucleated red blood cells in blood without increasing additional costs and decreasing detection efficiency.
To achieve the above object, a first aspect of the present application provides a blood cell analyzer comprising:
The sample sucking device is used for sucking a blood sample to be tested;
Sample preparation means for mixing a portion of the blood sample to be tested, a hemolytic agent and a first fluorescent dye to prepare a first assay sample for white blood cell count, and for mixing another portion of the blood sample to be tested, a diluent and a second fluorescent dye to prepare a second assay sample for identifying mature red blood cells, reticulocytes, platelets and white blood cells;
An optical detection device including a flow cell for passing the first measurement sample and the second measurement sample, respectively, a light source for irradiating the first measurement sample and the second measurement sample passing through the flow cell with light, and a light detector for detecting first optical information and second optical information generated after the first measurement sample and the second measurement sample are irradiated with light while passing through the flow cell, respectively; and
A data processing apparatus configured to:
obtaining a first white blood cell count result of the blood sample to be tested based on the first optical information,
Obtaining a second white blood cell count result of the blood sample to be tested based on the second optical information, and
And acquiring a nucleated red blood cell analysis result of the blood sample to be tested based on the first white blood cell count result and the second white blood cell count result.
In a second aspect, the application provides a method of blood cell analysis comprising:
sucking a blood sample to be tested;
Mixing a part of the blood sample to be measured, a hemolytic agent and a first fluorescent dye to prepare a first measurement sample for white blood cell counting, and passing particles in the first measurement sample one by one through an optical detection area irradiated with light to obtain first optical information generated by the particles in the first measurement sample after being irradiated with light;
Mixing another part of the blood sample to be measured, a diluent and a second fluorescent dye to prepare a second measurement sample for identifying mature red blood cells, reticulocytes, platelets and white blood cells, and passing particles in the second measurement sample one by one through an optical detection zone irradiated with light to obtain second optical information generated by the particles in the second measurement sample after being irradiated with light; and
And acquiring a nucleated red blood cell analysis result of the blood sample to be tested by combining the first optical information and the second optical information.
In the technical proposal provided by the aspects of the application, the first optical information obtained in the white blood cell counting detection channel and the second optical information obtained in the reticulocyte detection channel are combined, so that the alarm prompt of whether the nucleated red blood cells exist in the blood sample to be detected can be accurately given, without increasing the detection cost and reducing the detection efficiency.
Drawings
The application will be more clearly elucidated in connection with the examples and the accompanying drawings. The above-described and other advantages will become apparent to those skilled in the art from the detailed description of embodiments of the application. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the application.
In the drawings:
fig. 1 is a schematic exterior view of a blood cell analyzer according to some embodiments of the application.
Fig. 2 is a schematic block diagram of an optical detection device according to some embodiments of the application.
Fig. 3 is a FL-SS first white blood cell count scatter plot of a dog blood sample according to some embodiments of the present application.
FIG. 4 is a FS-SS second white blood cell count scattergram of the dog blood sample used in FIG. 3.
FIG. 5 is a third FS-FL scatter plot of the dog blood sample used in FIG. 3.
Fig. 6 is a schematic flow chart of a blood cell analysis method according to some embodiments of the application.
FIG. 7 is a schematic flow chart diagram of one embodiment of obtaining nucleated red blood cell analysis results in the blood cell analysis method shown in FIG. 6.
Detailed Description
Embodiments of the present application will now be described more fully hereinafter with reference to the accompanying drawings, in which it is shown, however, in which some, but not all embodiments of the application are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
It should be noted that, the term "first\second\third" related to the embodiment of the present application is merely to distinguish similar objects, and does not represent a specific order for the objects, it is to be understood that "first\second\third" may interchange a specific order or sequence where allowed.
It will be understood by those skilled in the art that all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs unless defined otherwise.
For convenience of the following description, some terms referred to below will be briefly described first:
1) Scatter plot: a two-or three-dimensional map generated by a blood cell analyzer having two or three-dimensional characteristic information of a plurality of particles distributed thereon. The X, Y and Z axes of the scatter plot each characterize a characteristic of each particle. For example, in a scatter plot, the X-axis represents forward scattered light intensity, the Y-axis represents fluorescence intensity, and the Z-axis represents side scattered light intensity.
2) Cell population: particle clusters formed by a plurality of particles having the same characteristics, such as a leukocyte population, and a neutrophil population, a lymphocyte population, a monocyte population, an eosinophil population, or a basophil population among leukocytes, which are distributed in a certain region of the scattergram.
3) Blood shadow: the hemolysis reagent dissolves the red blood cells and platelets in the blood to obtain the debris particles.
Currently, blood cell analyzers can test samples of human blood and other mammalian (e.g., dog, cat, horse, etc.) blood, avian blood, fish blood, etc. In general, a blood cell analyzer counts and classifies leukocytes through DIFF channels (leukocyte classification channels), for example, classifies leukocytes into five types of leukocytes, lymphocytes (Lym), monocytes (Mon), neutrophils (Neu), eosinophils (Eos), and basophils (Bas). Further, the blood cell analyzer obtains a reticulocyte count value, a red blood cell count value, a white blood cell count value, a platelet count value, and the like through a RET channel (reticulocyte detection channel).
The blood cell analyzer used in the embodiments of the present application classifies and counts particles in a sample by combining a laser scattering method and a flow cytometry of fluorescent staining. For example, the white blood cell count detection principle of a blood cell analyzer is: firstly sucking a blood sample, and treating the sample with a hemolyzing agent for white blood cell counting and a fluorescent dye, wherein red blood cells are destroyed and dissolved by the hemolyzing agent, white blood cells are not dissolved, but the fluorescent dye can enter cell nuclei of the white blood cells with the help of the hemolyzing agent and can be combined with nucleic acid substances in the cell nuclei; the particles in the blood sample then pass through the detection holes irradiated by the laser beam one by one, and when the laser beam irradiates the particles, the characteristics of the particles (such as volume, dyeing degree, cell content size and content, cell nucleus density and the like) can block or change the direction of the laser beam, so that scattered light with various angles corresponding to the characteristics of the scattered light can be generated, and the scattered light can obtain relevant information on the structure and composition of the particles after being received by the signal detector. Among them, forward Scatter (FS) reflects the number and volume of particles, side scatter (SIDE SCATTER, SS) reflects the complexity of the cell internal structures (e.g., intracellular particles or nuclei), and Fluorescence (FL) reflects the content of nucleic acid substances in the cell. The light information can be used to classify and count particles in the sample.
Fig. 1 is a schematic diagram of a blood cell analyzer according to some embodiments of the application. The blood cell analyzer 100 includes a sample-sucking device 110, a sample preparation device 120, an optical detection device 130, and a data processing device 140. The blood cell analyzer 100 also has a fluid path system, not shown, for communicating the sample application device 110, the sample preparation device 120, and the optical detection device 130 for fluid transfer between these devices.
The sample sucking device 110 is used for sucking a blood sample to be tested.
In some embodiments, the sample-absorbing device 110 has a sampling needle (not shown) for absorbing a blood sample to be tested. Furthermore, the sample suction device 110 may comprise, for example, a drive device for driving the sampling needle to quantitatively suck the blood sample to be measured through the mouth of the sampling needle. The sample aspiration device 110 may deliver the collected blood sample to the sample preparation device 120.
The sample preparation device 120 is used for mixing a portion of a blood sample to be tested, a hemolytic agent and a first fluorescent dye to prepare a first assay sample for white blood cell count, and for mixing another portion of the blood sample to be tested, a diluent and a second fluorescent dye to prepare a second assay sample for identifying mature red blood cells, reticulocytes, platelets and white blood cells.
In the embodiment of the application, the hemolytic agent is used for dissolving red blood cells in blood, the red blood cells are cracked into fragments, and white blood cells shrink under the action of the hemolytic agent, so that the volume is reduced.
In an embodiment of the present application, the first fluorescent dye is a fluorescent dye for staining DNA in leukocytes for achieving white blood cell count, and may be, for example, a fluorescent dye capable of achieving classification of leukocytes in a blood sample into five leukocyte subpopulations (neutrophils, lymphocytes, monocytes, eosinophils and basophils). The second fluorescent dye is different from the first fluorescent dye and stains RNA in the cells for obtaining a mature red blood cell count value, a reticulocyte count value, a platelet count value, a granulocyte count value, a lymphocyte count value, and a monocyte count value.
In some embodiments, the sample preparation device 120 may include at least one reaction cell and a reagent supply device (not shown). The at least one reaction cell is for receiving the blood sample to be measured sucked by the sample sucking means 110, and the reagent supplying means supplies a processing reagent (including a hemolyzing agent, a diluent, a first fluorescent dye, a second fluorescent dye, etc.) to the at least one reaction cell, so that the blood sample to be measured sucked by the sample sucking means 110 is mixed with the processing reagent supplied by the reagent supplying means in the reaction cell to prepare a measurement sample (including a first measurement sample and a second measurement sample).
For example, the at least one reaction cell may include a first reaction cell and a second reaction cell, and the reagent supply device may include a first reagent supply part and a second reagent supply part. The sample absorbing device 110 is used for respectively and partially distributing the collected blood sample to be tested to the first reaction tank and the second reaction tank. The first reagent supply section is configured to supply the hemolysis agent and the first fluorescent dye to the first reaction cell, so that a portion of the blood sample to be measured, which is allocated to the first reaction cell, is mixed and reacted with the hemolysis agent and the first fluorescent dye to prepare a first measurement sample. The second reagent supply section is configured to supply a second fluorescent dye and a diluent, for example, a low osmotic pressure diluent (for example, for spheroidizing red blood cells) to the second reaction cell, so that a portion of the blood sample to be measured, which is dispensed to the second reaction cell, is mixed and reacted with the second fluorescent dye and the optional diluent to prepare a second measurement sample.
The optical detection device 130 includes a flow cell for passing the first measurement sample and the second measurement sample, respectively, a light source for irradiating the first measurement sample and the second measurement sample, respectively, passing through the flow cell with light, and a light detector for detecting first optical information and second optical information generated after the first measurement sample and the second measurement sample, respectively, are irradiated with light while passing through the flow cell.
It will be understood herein that the first detection channel (also referred to as DIFF channel) for white blood cell count refers to the detection of a first assay sample prepared by the sample preparation device 120 by the optical detection device 130, and the second detection channel (also referred to as RET channel) for the identification of mature red blood cells, reticulocytes, platelets, and white blood cells refers to the detection of a second assay sample prepared by the sample preparation device 120 by the optical detection device 130.
In this context, a flow cell refers to a chamber adapted to detect focused liquid flow of light scattering signals and fluorescent signals. When a particle, such as a blood cell, passes through the detection aperture of the flow cell, the particle scatters the incident light beam from the light source directed toward the detection aperture in various directions. The light detector may be arranged at one or more different angles relative to the incident light beam to detect light scattered by the particles to obtain a light scattering signal. Since different particles have different light scattering properties, the light scattering signal can be used to distinguish between different populations of particles. In particular, the light scattering signal detected in the vicinity of the incident light beam is generally referred to as a forward light scattering signal or a small angle light scattering signal. In some embodiments, the forward light scatter signal may be detected from an angle of about 1 ° to about 4 ° from the incident light beam. In other embodiments, the forward light scatter signal may be detected from an angle of about 2 ° to about 6 ° from the incident light beam. The light scattering signal detected in a direction at about 90 ° to the incident light beam is generally referred to as a side light scattering signal. In some embodiments, the side scatter signal may be detected from an angle of about 65 ° to about 115 ° from the incident light beam. Typically, fluorescent signals from blood cells stained with a fluorescent dye are also typically detected in a direction that is about 90 ° from the incident light beam.
In some embodiments, the light detector may include a forward scatter light detector for detecting forward scatter light signals, a side scatter light detector for detecting side scatter light signals, and a fluorescence light detector for detecting fluorescence signals. Accordingly, the first optical information may include forward scattered light signals, side scattered light signals, and fluorescent signals of the particles in the first assay sample, and the second optical information may include forward scattered light signals, side scattered light signals, and fluorescent signals of the particles in the second assay sample.
Fig. 2 shows a specific example of the optical detection device 130. The optical detection device 130 has a light source 101, a beam shaping assembly 102, a flow cell 103 and a forward scatter detector 104 arranged in that order in a straight line. On one side of the flow chamber 103, a dichroic mirror 106 is arranged at an angle of 45 ° to the straight line. A part of the lateral light emitted by the particles in the flow cell 103 is transmitted through the dichroic mirror 106, and is captured by the fluorescence detector 105 arranged behind the dichroic mirror 106 at an angle of 45 ° to the dichroic mirror 106; another portion of the side light is reflected by the dichroic mirror 106 and captured by a side scatter detector 107 arranged in front of the dichroic mirror 106 at an angle of 45 ° to the dichroic mirror 106.
The data processing device 140 is configured to process and calculate data to obtain a desired result, and for example, may generate a two-dimensional scattergram or a three-dimensional scattergram from various collected optical signals, and perform particle analysis on the scattergram according to a gating (gating) method. The data processing device 140 may also perform visualization processing on the intermediate operation result or the final operation result, and then display the result on the display device 150. In an embodiment of the application, the data processing device 140 is configured for carrying out the method steps described in further detail below.
In an embodiment of the application, the data processing device includes, but is not limited to, a central processing unit (Central Processing Unit, CPU), a micro control unit (Micro Controller Unit, MCU), a field-programmable gate array (field-programmable GATE ARRAY, FPGA), a digital signal processing Device (DSP), etc. for interpreting computer instructions and processing data in computer software. For example, the data processing device is configured to execute each computer application program in the computer readable storage medium, so that the blood cell analyzer 100 executes a corresponding detection procedure and analyzes the optical information or optical signal detected by the optical detection device 130 in real time.
In addition, the blood cell analyzer 100 may further include a first housing 160 and a second housing 170. The display device 150 may be, for example, a user interface. The optical detection device 130 and the data processing device 140 are disposed inside the second housing 170. The sample preparation device 120 is disposed inside the first housing 160, for example, and the display device 150 is disposed on an outer surface of the first housing 160 and is used to display a detection result of the blood cell analyzer, for example.
As mentioned in the background art, in order to identify nucleated red blood cells in blood without increasing the detection cost and reducing the detection efficiency, it is proposed to perform the identification of nucleated red blood cells in the existing DIFF channel or RET channel. However, the inventors of the present application found that in the white blood cell count scattergram obtained from the DIFF channel, there is an overlap of nucleated red blood cells with other cell populations, particularly neutrophil populations, resulting in an inability to accurately identify nucleated red blood cells, and further an inability to obtain accurate nucleated red blood cell count values; in the reticulocyte scatter diagram obtained from the RET channel, the nucleated red blood cell distribution area overlaps with other cell groups, particularly the reticulocyte distribution area, resulting in an inaccurate identification of nucleated red blood cells and an even more inaccurate nucleated red blood cell count value.
Based on this, the embodiment of the present application proposes to identify nucleated red blood cells in a blood sample to be measured by combining the first optical information obtained in the DIFF channel and the second optical information obtained in the RET channel, so as to obtain a more accurate nucleated red blood cell alarm prompt and optionally a more accurate nucleated red blood cell count value.
It is proposed herein that the data processing device 140 is configured to obtain the nucleated red blood cell analysis result of the blood sample to be tested in combination with the first optical information and the second optical information.
In some embodiments, the data processing apparatus 140 may be configured to:
acquiring a first white blood cell count result of a blood sample to be tested based on the first optical information;
acquiring a second white blood cell count result of the blood sample to be measured based on the second optical information; and
And obtaining a nucleated red blood cell analysis result of the blood sample to be tested based on the first white blood cell count result and the second white blood cell count result.
In some embodiments, the first white blood cell count result comprises a lymphocyte count value, a monocyte count value, a neutrophil count value, an eosinophil count value, and a basophil count value; and the second white blood cell count result includes a granulocyte count value, a monocyte count value, and a lymphocyte count value, wherein the granulocyte count value includes a neutrophil count value, an eosinophil count value, and a basophil count value.
In some embodiments, as shown in fig. 3, the data processing device 140 obtaining the first white blood cell count result of the blood sample to be tested based on the first optical information may include: data processing device 140
Generating a first white blood cell count scattergram based on at least the side scatter light signal SS and the side fluorescence signal FL in the first optical information; and
A first white blood cell count result is obtained based on a first white blood cell count scattergram, the first white blood cell count result including a neutrophil diff_neu count value, a lymphocyte diff_lym count value, a monocyte diff_mon count value, an eosinophil diff_eos count value, and a basophil diff_baso count value.
In some embodiments, as shown in fig. 4, the data processing device 140 obtaining the second white blood cell count result of the blood sample to be tested based on the second optical information may include: data processing device 140
Generating a second white blood cell count scattergram based on at least the forward scattered light signal FS and the side scattered light signal SS in the second optical information; and
Obtaining a second white blood cell count result based on the second white blood cell count scattergram, the second white blood cell count result comprising a lymphocyte count value, a monocyte ret_mon count value, and a granulocyte ret_gran count value, the granulocytes comprising neutrophils ret_neu, eosinophils ret_eos, and basophils ret_bas.
The red cell count value is calibrated with the target value of red cells in the calibrator in the RET channel, and the white cell count value is calibrated with the white cell count value in the RET channel.
The inventors of the present application found that, in the scatter diagram shown in fig. 3, nucleated red blood cells NRBC are included in the neutrophil group Neu, and that when nucleated red blood cells of the blood sample to be measured are increased, both the total number of white blood cells WBCs and the neutrophil Neu count value are increased. In the scatter diagram shown in fig. 4, there is no nucleated red blood cell NRBC in the granulocyte population ret_gran.
Thus, in some embodiments, the data processing device 140 obtaining the nucleated red blood cell analysis result of the blood sample to be measured based on the first white blood cell count result and the second white blood cell count result may include:
The data processing device 140 obtains a nucleated red blood cell analysis result of the blood sample to be measured based on the neutrophil count value, the eosinophil count value, and the basophil count value of the first white blood cell count result, and the granulocyte count value of the second white blood cell count result. .
For example, when the difference of the neutrophil count value, the sum of the eosinophil count value and the basophil count value in the first white blood cell count result minus the granulocyte count value in the second white blood cell count result is greater than a first preset threshold, i.e., diff_neu (neutrophil count) +diff_eos (eosinophil count) +diff_bas (basophil count) -ret_grand (granulocyte count) > T1 (first preset threshold), the data processing apparatus outputs an indication of the presence of nucleated red blood cells in the blood sample to be tested.
Further, the data processing apparatus obtaining the nucleated red blood cell analysis result of the blood sample to be measured based on the neutrophil count value, the eosinophil count value, and the basophil count value in the first white blood cell count result, and the granulocyte count value in the second white blood cell count result may include: data processing device 140
When the difference of the neutrophil count value, the sum of the eosinophil count value and the basophil count value in the first white blood cell count result minus the granulocyte count value in the second white blood cell count result is greater than a second preset threshold, that is, diff_neu (neutrophil count) +diff_eos (eosinophil count) +diff_bas) -ret_grand (granulocyte count) > T2 (second preset threshold), the difference is output as the nucleated red blood cell count value of the blood sample to be measured.
In some embodiments, the second preset threshold is greater than the first preset threshold.
In some embodiments, as shown in FIG. 5, the data processing device 140 may be further configured to generate a third scatter plot based on at least the forward scattered light signal FS and the side fluorescence signal FL in the second optical information; and
Obtaining a red blood cell count value, a platelet count value, a reticulocyte count value, and a reticulocyte classification based on the third scatter plot, wherein the reticulocyte classification includes low fluorescence reticulocytes, medium fluorescence reticulocytes, and high fluorescence reticulocytes.
Accordingly, as shown in fig. 6, an embodiment of the present application further provides a blood cell analysis method 200, including:
s210, sucking a blood sample to be tested;
s220, mixing a part of the blood sample to be tested, a hemolytic agent (for lysing red blood cells in blood, and fragments after lysing do not affect the counting and classification of white blood cells) and a first staining agent (for staining DNA in white blood cells) to prepare a first measurement sample for white blood cell counting, and passing particles in the first measurement sample one by one through an optical detection area irradiated with light to obtain first optical information generated by the particles in the first measurement sample after being irradiated with light;
S230, mixing another part of the blood sample to be tested, a diluent and a second fluorescent staining agent (for staining RNA in reticulocytes, red blood cells and platelets) to prepare a second measurement sample for identifying mature red blood cells, reticulocytes, platelets and white blood cells, and passing particles in the second measurement sample one by one through an optical detection zone irradiated with light to obtain second optical information generated by the particles in the second measurement sample after being irradiated with light; and
S240, acquiring a nucleated red blood cell analysis result of the blood sample to be tested by combining the first optical information and the second optical information.
In some embodiments, as shown in fig. 7, step S240 may include:
s241, acquiring a first white blood cell count result of the blood sample to be tested based on the first optical information, wherein the first white blood cell count result comprises a lymphocyte count value, a monocyte count value, a neutrophil count value, an eosinophil count value and a basophil count value;
S242, obtaining a second white blood cell count result of the blood sample to be tested based on the second optical information, wherein the second white blood cell count result comprises a granulocyte count value, a monocyte count value and a lymphocyte count value, and the granulocytes comprise neutrophils, eosinophils and basophils; and
S243, obtaining a nucleated red blood cell analysis result of the blood sample to be tested based on the first white blood cell count result and the second white blood cell count result.
In some embodiments, step S243 may include: and acquiring a nucleated red blood cell analysis result of the blood sample to be tested based on the neutrophil count value, the eosinophil count value and the basophil count value in the first white blood cell count result and the granulocyte count value in the second white blood cell count result.
Further, when the difference of the sum of the neutrophil count value, the eosinophil count value and the basophil count value in the first white blood cell count result minus the granulocyte count value in the second white blood cell count result is greater than a first preset threshold, outputting a prompt that the nucleated red blood cells exist in the blood sample to be detected.
Further, when the difference of the sum of the neutrophil count value, the eosinophil count value and the basophil count value in the first white blood cell count result minus the granulocyte count value in the second white blood cell count result is greater than a second preset threshold, outputting the difference as the nucleated red blood cell count of the blood sample to be measured.
In some embodiments, step S241 may include:
Generating a first white blood cell count scattergram based on at least the side scatter light signal and the side fluorescence signal in the first optical information; and
The first white blood cell classification result is obtained based on the first white blood cell count scattergram, the first white blood cell count result including a neutrophil count value, a lymphocyte count value, a monocyte count value, an eosinophil count value ratio, and a basophil count value.
In some embodiments, step S242 may include:
Generating a second white blood cell count scattergram based on at least the forward scattered light signal and the side scattered light signal in the second optical information; and
Obtaining the second white blood cell count result based on the second white blood cell count scattergram, the second white blood cell count result comprising a lymphocyte count value, a monocyte count value, and a granulocyte count value, wherein the granulocytes comprise neutrophils, eosinophils, and basophils.
Further embodiments of the blood cell analysis method 200 and advantages thereof according to the embodiments of the present application can be referred to the above description of the blood cell analyzer 100, and will not be repeated here.
The features or combinations of features mentioned above in the description, in the drawings and in the claims may be used in any combination with one another or individually, as long as they are significant and do not contradict one another within the scope of the application.
The foregoing description is only of the preferred embodiments of the present application and is not intended to limit the scope of the application, and all equivalent modifications made by the present application and the accompanying drawings, or direct/indirect application in other related technical fields are included in the scope of the present application.
Claims (14)
1. A blood cell analyzer, comprising:
The sample sucking device is used for sucking a blood sample to be tested;
Sample preparation means for mixing a portion of the blood sample to be tested, a hemolytic agent and a first fluorescent dye to prepare a first assay sample for white blood cell count, and for mixing another portion of the blood sample to be tested, a diluent and a second fluorescent dye to prepare a second assay sample for identifying mature red blood cells, reticulocytes, platelets and white blood cells;
An optical detection device including a flow cell for passing the first measurement sample and the second measurement sample, respectively, a light source for irradiating the first measurement sample and the second measurement sample passing through the flow cell with light, and a light detector for detecting first optical information and second optical information generated after the first measurement sample and the second measurement sample are irradiated with light while passing through the flow cell, respectively; and
A data processing apparatus configured to:
obtaining a first white blood cell count result of the blood sample to be tested based on the first optical information,
Obtaining a second white blood cell count result of the blood sample to be tested based on the second optical information, and
And acquiring a nucleated red blood cell analysis result of the blood sample to be tested based on the first white blood cell count result and the second white blood cell count result.
2. The blood cell analyzer of claim 1, wherein the first white blood cell count result comprises a lymphocyte count value, a monocyte count value, a neutrophil count value, an eosinophil count value, and a basophil count value; and the second white blood cell count result includes a granulocyte count value, a monocyte count value, and a lymphocyte count value, wherein the granulocytes include neutrophils, eosinophils, and basophils.
3. The blood cell analyzer of claim 2, wherein the data processing device obtains a nucleated red blood cell analysis result of the blood sample to be measured based on the first white blood cell count result and the second white blood cell count result, comprising:
the data processing device obtains a nucleated red blood cell analysis result of the blood sample to be measured based on a neutrophil count value, an eosinophil count value, and a basophil count value in the first white blood cell count result, and a granulocyte count value in the second white blood cell count result.
4. A blood cell analyzer according to claim 3, wherein the data processing means obtains a nucleated red blood cell analysis result of the blood sample to be measured based on a neutrophil count value, an eosinophil count value, and a basophil count value of the first white blood cell count result, and a granulocyte count value of the second white blood cell count result, comprising: the data processing device
Outputting a prompt of the presence of nucleated red blood cells in the blood sample to be detected when the difference of the neutrophil count value, the sum of the eosinophil count value and the basophil count value in the first white blood cell count result minus the granulocyte count value in the second white blood cell count result is greater than a first preset threshold.
5. The blood cell analyzer according to claim 3 or 4, wherein the data processing device acquires a nucleated red blood cell analysis result of the blood sample to be measured based on a neutrophil count value, an eosinophil count value, and a basophil count value in the first white blood cell count result, and a granulocyte count value in the second white blood cell count result, comprising: the data processing device
Outputting the difference value as the nucleated red blood cell count of the blood sample to be detected when the difference value of the neutrophil count value, the eosinophil count value and the basophil count value in the first white blood cell count result minus the granulocyte count value in the second white blood cell count result is larger than a second preset threshold value.
6. The blood cell analyzer of any one of claims 1 to 5, wherein the data processing device obtains a first white blood cell count result of the blood sample under test based on the first optical information, comprising: the data processing device
Generating a first white blood cell count scattergram based on at least the side scatter light signal and the side fluorescence signal in the first optical information; and
Obtaining the first white blood cell count result based on the first white blood cell count scattergram, the first white blood cell count result comprising a lymphocyte count value, a monocyte count value, a neutrophil count value, an eosinophil count value, and a basophil count value.
7. The blood cell analyzer according to any one of claims 1 to 6, wherein the data processing device acquires a second white blood cell count result of the blood sample to be measured based on the second optical information, comprising: the data processing device
Generating a second white blood cell count scattergram based on at least the forward scattered light signal and the side scattered light signal in the second optical information; and
Obtaining the second white blood cell count result based on the second white blood cell count scattergram, the second white blood cell count result comprising a granulocyte count value, a monocyte count value, and a lymphocyte count value, wherein the granulocytes comprise neutrophils, eosinophils, and basophils.
8. A method of blood cell analysis comprising:
sucking a blood sample to be tested;
Mixing a part of the blood sample to be measured, a hemolytic agent and a first fluorescent dye to prepare a first measurement sample for white blood cell counting, and passing particles in the first measurement sample one by one through an optical detection area irradiated with light to obtain first optical information generated by the particles in the first measurement sample after being irradiated with light;
Mixing another part of the blood sample to be measured, a diluent and a second fluorescent dye to prepare a second measurement sample for identifying mature red blood cells, reticulocytes, platelets and white blood cells, and passing particles in the second measurement sample one by one through an optical detection zone irradiated with light to obtain second optical information generated by the particles in the second measurement sample after being irradiated with light; and
And acquiring a nucleated red blood cell analysis result of the blood sample to be tested by combining the first optical information and the second optical information.
9. The method of claim 8, wherein obtaining the nucleated red blood cell analysis result of the blood sample to be measured in combination with the first optical information and the second optical information comprises:
Obtaining a first white blood cell count result of the blood sample to be tested based on the first optical information, wherein the first white blood cell count result comprises a lymphocyte count value, a monocyte count value, a neutrophil count value, an eosinophil count value and a basophil count value;
Obtaining a second white blood cell count result of the blood sample to be tested based on the second optical information, the second white blood cell count result comprising a granulocyte count value, a monocyte count value, and a lymphocyte count value, wherein the granulocytes comprise neutrophils, eosinophils, and basophils; and
And acquiring a nucleated red blood cell analysis result of the blood sample to be tested based on the first white blood cell count result and the second white blood cell count result.
10. The blood cell analysis method according to claim 9, wherein obtaining the nucleated red blood cell analysis result of the blood sample to be measured based on the first white blood cell count result and the second white blood cell count result comprises:
and acquiring a nucleated red blood cell analysis result of the blood sample to be tested based on the neutrophil count value, the eosinophil count value and the basophil count value in the first white blood cell count result and the granulocyte count value in the second white blood cell count result.
11. The method according to claim 10, wherein obtaining the nucleated red blood cell analysis result of the blood sample to be tested based on the neutrophil count value, the eosinophil count value, and the basophil count value in the first white blood cell count result, and the granulocyte count value in the second white blood cell count result, comprises:
outputting a prompt of the presence of nucleated red blood cells in the blood sample to be detected when the difference of the neutrophil count value, the eosinophil count value and the sum of the eosinophil count value and the basophil count value in the first white blood cell count result minus the granulocyte count value in the second white blood cell count result is greater than a first preset threshold.
12. The blood cell analysis method according to claim 10 or 11, wherein obtaining the nucleated red blood cell analysis result of the blood sample to be measured based on the neutrophil count value, the eosinophil count value, and the basophil count value in the first white blood cell count result, and the granulocyte count value in the second white blood cell count result, comprises:
Outputting the difference value as the nucleated red blood cell count of the blood sample to be detected when the difference value of the neutrophil count value, the eosinophil count value and the basophil count value in the first white blood cell count result minus the granulocyte count value in the second white blood cell count result is larger than a second preset threshold value.
13. The blood cell analysis method according to any one of claims 9 to 12, wherein obtaining a first white blood cell count result of the blood sample to be tested based on the first optical information comprises:
Generating a first white blood cell count scattergram based on at least the side scatter light signal and the side fluorescence signal in the first optical information; and
Obtaining the first white blood cell count result based on the first white blood cell count scattergram, the first white blood cell count result comprising a lymphocyte count value, a monocyte count value, a neutrophil count value, an eosinophil count value, and a basophil count value.
14. The blood cell analysis method according to any one of claims 9 to 13, wherein obtaining a second white blood cell count result of the blood sample to be tested based on the second optical information comprises:
Generating a second white blood cell count scattergram based on at least the forward scattered light signal and the side scattered light signal in the second optical information; and
Obtaining the second white blood cell count result based on the second white blood cell count scattergram, the second white blood cell count result comprising a granulocyte count value, a monocyte count value, and a lymphocyte count value, wherein the granulocyte count value comprises neutrophils, eosinophils, and basophils.
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