CN117848461A - Correction method, system and analyzer for liquid suction position of reagent needle - Google Patents
Correction method, system and analyzer for liquid suction position of reagent needle Download PDFInfo
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Abstract
The invention provides a correction method, a correction system and an analyzer for the liquid suction position of a reagent needle, and relates to the technical field of liquid level detection. The method comprises the following steps: acquiring the initial movement step number of a reagent needle and the theoretical suction quantity of a kit, and calculating the current theoretical movement step number of the reagent needle according to the initial movement step number and the theoretical suction quantity; acquiring the current detection movement step number of the nth sample suction of the reagent needle; calculating an actual motion step number difference value between the current theoretical motion step number and the current detection motion step number; obtaining M monitoring detection movement steps according to the actual movement step difference value and a tolerable difference threshold; acquiring L limit detection motion steps based on the M monitoring detection motion steps and the actual detection motion step threshold; and acquiring the target updating theoretical movement steps based on the M monitoring detection movement steps or the L limit detection movement steps. Therefore, the current real liquid level position can be automatically corrected by monitoring, and the working efficiency is ensured.
Description
Technical Field
The invention relates to the technical field of liquid level detection, in particular to a method and a system for correcting the liquid suction position of a reagent needle and an analyzer.
Background
When the liquid level detection technology is used at present for detecting the liquid level position, on one hand, the influence of bubbles during initial detection cannot be avoided, so that reagent is sucked in error, and a large number of test results are inaccurate, and meanwhile, if the liquid level position is abnormal during the test, a large number of test results are wrong; on the other hand, in order to ensure stability of the detection result, the speed of the reagent sucking process of the reagent needle needs to be controlled, and the test efficiency of the device is sacrificed if each detection is performed.
Disclosure of Invention
In view of the above, the present invention aims to overcome the defects in the prior art, and provides a method, a system and an analyzer for correcting a liquid suction position of a reagent needle, which are used for detecting a liquid level position through a reagent kit in a monitoring test process, judging whether a current liquid level position state is abnormal through comparison with a theoretical position, further judging whether a special treatment process is triggered, confirming a current reliable actual liquid level position, and further judging whether the reagent suction position of the reagent needle is corrected or the reagent kit is replaced.
The invention provides the following technical scheme:
in a first aspect, the present invention provides a method of correcting the pipetting position of a reagent needle, the method comprising:
Acquiring the initial movement step number of a reagent needle and the theoretical suction quantity of a kit, and calculating the current theoretical movement step number of the reagent needle according to the initial movement step number and the theoretical suction quantity;
acquiring the current detection movement step number of the nth sample suction of the reagent needle;
calculating an actual motion step number difference value between the current theoretical motion step number and the current detection motion step number;
obtaining M monitoring detection movement steps according to the actual movement step difference value and a tolerable difference threshold;
acquiring L limit detection motion steps based on the M monitoring detection motion steps and the actual detection motion step threshold;
and acquiring the target updating theoretical movement step number based on the M monitoring detection movement step numbers or the L limit detection movement step numbers.
In an embodiment, the obtaining M monitoring detected motion steps according to the actual motion step difference value and the tolerable difference threshold includes:
comparing the actual motion step number difference value with a tolerable difference threshold;
and if the actual movement step number difference value is larger than the tolerable difference threshold, continuously monitoring the sample suction of the reagent needle for M times to obtain M monitoring detection movement step numbers.
In an embodiment, the target update theoretical moving step number includes a first update theoretical moving step number, and the obtaining the target update theoretical moving step number based on the M monitoring detected moving step numbers or the L limit detected moving step numbers includes:
calculating a limit detection motion step number average value and a second variation coefficient according to the L limit detection motion step numbers;
comparing the average value of the limit detection motion steps with the threshold value of the actual detection motion steps;
if the average value of the limit detection motion steps is larger than the threshold value of the actual detection motion steps, comparing the second variation coefficient with the threshold value of the variation coefficient;
and if the second variation coefficient is smaller than or equal to the variation coefficient threshold value, obtaining the first updated theoretical number of motion steps according to the limit detection motion step number average value.
In an embodiment, the obtaining the L limit detection motion steps based on the M monitor detection motion steps includes:
calculating the average value of the step numbers of the monitoring detection motion according to the M step numbers of the monitoring detection motion;
comparing the average value of the step number of the monitoring detection motion with an actual step number threshold value of the detection motion;
if the average value of the monitoring detection movement steps is smaller than or equal to the threshold value of the actual detection movement steps, the reagent needle is subjected to L times of sample suction by moving to the limit position, and L limit detection movement steps are obtained.
In an embodiment, the obtaining the first updated theoretical number of motion steps based on the L limit detected number of motion steps includes:
calculating a limit detection motion step number average value and a second variation coefficient according to the L limit detection motion step numbers;
comparing the average value of the limit detection motion steps with an actual detection motion step threshold value;
if the average value of the limit detection motion steps is larger than the threshold value of the actual detection motion steps, comparing the second variation coefficient with the threshold value of the variation coefficient;
and if the second variation coefficient is smaller than or equal to the variation coefficient threshold value, obtaining the first updated theoretical number of motion steps according to the limit detection motion step number average value.
In an embodiment, the target updated theoretical number of motion steps further includes a second updated theoretical number of motion steps, and the obtaining the target updated theoretical number of motion steps based on the M monitored detected number of motion steps or the L limit detected number of motion steps further includes:
if the average value of the monitoring detection motion steps is larger than the actual detection motion step number threshold value, calculating a first variation coefficient according to M monitoring detection motion steps, and comparing the first variation coefficient with the actual detection motion step number threshold value;
If the first variation coefficient is smaller than or equal to the actual detection motion step number threshold value, not updating the current theoretical motion step number;
and if the first variation coefficient is larger than the actual detection motion step number threshold value, obtaining a second updated theoretical motion step number according to the monitoring detection motion step number average value.
In one embodiment, at least one of the following features (1) - (3) is included:
(1) Said calculating a current theoretical number of steps of movement of said reagent needle based on said initial number of steps of movement and said theoretical amount of aspiration, comprising:
determining the product of the theoretical suction quantity and N-1 to obtain a target product;
determining the sum value of the target product and the target coefficient to obtain a target sum value;
determining the sum value of the initial motion step number and the target sum value to obtain the current theoretical motion step number;
(2) The step number average value of the motion steps according to the limit detection is used for obtaining a first updated theoretical motion step number, and the step number average value comprises the following steps: determining the sum value of the limit detection motion step number average value and the target sum value to obtain the first updated theoretical motion step number;
(3) The step number obtaining a second updated theoretical step number according to the average value of the step numbers of the monitoring detection motion comprises the following steps: and determining the sum value of the average value of the monitoring detection motion steps and the target sum value to obtain the second updated theoretical motion steps.
In one embodiment, the obtaining the initial number of steps of movement of the reagent needle and the theoretical amount of aspiration of the reagent cartridge comprises:
the liquid level position information is obtained when the kit is loaded;
determining the initial number of movement steps according to the liquid level position information;
and determining the theoretical suction amount according to the item information of the kit.
In one embodiment, the step of calculating the first coefficient of variation according to the M monitored detected motion steps includes: and calculating the difference value of the adjacent two monitoring detection movement steps to obtain M-1 monitoring movement step difference values, and calculating the first variation coefficient according to the M-1 monitoring movement step difference values.
In one embodiment, the method comprises at least one of the following features (1) - (3):
(1) The step of calculating the second coefficient of variation from the L limit detection motion steps includes: and calculating the difference value of the adjacent two limit detection movement steps to obtain L-1 limit movement step difference values, and calculating the second variation coefficient according to the L-1 limit movement step difference values.
(2) After comparing the limit detection motion step number average value with the actual detection motion step number threshold value, the method further comprises the following steps: if the average value of the limit detection movement steps is smaller than the threshold value of the actual detection movement steps, alarming that the test of the kit is abnormal, and disabling the kit;
(3) After comparing the second coefficient of variation with the coefficient of variation threshold, the method further comprises: and if the second variation coefficient is larger than the variation coefficient threshold value, alarming that the test of the kit is abnormal, and disabling the kit.
In an embodiment, after comparing the actual motion step number difference value with the tolerable difference threshold, the method further includes: and if the actual motion step number difference value is smaller than or equal to the tolerable difference threshold, not updating the current theoretical motion step number.
In a second aspect, the present invention provides a system for correcting the pipetting position of a reagent needle, the system comprising:
a kit for storing reagents;
the control device is used for acquiring the initial movement steps of the reagent needle and the theoretical suction quantity of the reagent kit; calculating the current theoretical movement steps of the reagent needle according to the initial movement steps and the theoretical suction amount;
the reagent needle is in communication connection with the control device and is used for sucking the reagent in the kit according to the current theoretical number of movement steps;
the liquid level detection device is positioned at the needle point of the reagent needle, is in communication connection with the control device and is used for synchronously detecting the current detection movement steps of the nth sample suction of the reagent needle;
The control device is also used for acquiring the current detection movement steps of the nth sample suction of the reagent needle from the liquid level detection device; calculating an actual motion step number difference value between the current theoretical motion step number and the current detection motion step number; obtaining M monitoring detection movement steps according to the actual movement step difference value and a tolerable difference threshold; acquiring L limit detection motion steps based on the M monitoring detection motion steps and the actual detection motion step threshold; and acquiring the target updating theoretical movement step number based on the M monitoring detection movement step numbers or the L limit detection movement step numbers.
In one embodiment, at least one of the following features (1) - (2) is included:
(1) The control device is also used for comparing the actual motion step number difference value with a tolerable difference threshold; if the actual motion step number difference value is larger than the tolerable difference threshold, continuously monitoring the sample suction of the reagent needle for M times;
the liquid level detection device is also used for synchronously detecting M monitoring detection movement steps of the reagent needle during M times of sample suction;
the control device is also used for acquiring M monitoring detection movement steps from the liquid level detection device;
(2) The control device is further configured to not update the current theoretical number of steps of motion if the actual number of steps of motion difference is less than or equal to the tolerable difference threshold.
In one embodiment, at least one of the following features (1) - (3) is included:
(1) The control device is also used for calculating the average value of the monitoring detection motion steps according to the M monitoring detection motion steps; comparing the average value of the step number of the monitoring detection motion with the threshold value of the step number of the actual detection motion; if the average value of the step numbers of the monitoring detection motion is smaller than or equal to the threshold value of the step numbers of the actual detection motion, controlling the reagent needle to perform L times of sample suction by moving to the limit position;
the liquid level detection device is also used for synchronously detecting L limit detection movement steps of the reagent needle during L times of sample suction;
the control device is also used for acquiring L limit detection motion steps;
the target updating theoretical movement step number comprises a first updating theoretical movement step number, and the control device is further used for respectively calculating a limit detection movement step number average value and a second variation coefficient according to the L limit detection movement step numbers; comparing the average value of the limit detection motion steps with an actual detection motion step threshold value; if the average value of the limit detection motion steps is larger than the threshold value of the actual detection motion steps, comparing the second variation coefficient with the threshold value of the variation coefficient; if the second variation coefficient is smaller than or equal to the variation coefficient threshold value, obtaining the first updated theoretical number of motion steps according to the limit detection motion step number average value;
The reagent needle is also used for sucking samples according to the first updated theoretical movement steps;
the control device is further used for determining the sum value of the limit detection motion step number average value and the target sum value to obtain the first updated theoretical motion step number; the step of calculating the second coefficient of variation from the L limit detection motion steps comprises: calculating the difference value of two adjacent limit detection motion steps to obtain L-1 limit motion step difference values, and calculating the second variation coefficient according to the L-1 limit motion step difference values;
(2) The control device is further used for alarming that the kit is abnormal in test and disabling the kit if the second variation coefficient is larger than the variation coefficient threshold;
(3) And the control device is also used for alarming the test abnormality of the kit and disabling the kit if the average value of the number of steps of the limit detection motion is smaller than or equal to the threshold value of the number of steps of the actual detection motion.
In an embodiment, the target updated theoretical number of steps of motion further includes a second updated theoretical number of steps of motion, and the control device is further configured to calculate a first coefficient of variation according to M monitored detected number of steps of motion if the monitored detected number of steps of motion average value is greater than the actual detected number of steps of motion threshold value, and compare the first coefficient of variation with the actual detected number of steps of motion threshold value; if the first variation coefficient is smaller than or equal to the actual detection motion step number threshold value, not updating the current theoretical motion step number; if the first variation coefficient is larger than the actual detection motion step number threshold value, obtaining a second updated theoretical motion step number according to the monitoring detection motion step number average value; determining the sum value of the average value of the monitoring detection motion steps and the target sum value to obtain the second updated theoretical motion steps;
The reagent needle is also used for sucking samples from the reagent kit according to the second updated theoretical number of movement steps;
the control device is also used for calculating the difference value of the adjacent two monitoring detection movement steps to obtain M-1 monitoring movement step difference values, and calculating a first variation coefficient according to the M-1 monitoring movement step difference values.
In a third aspect, the invention provides an analyzer comprising the correction system of the pipetting position of the reagent needle of the second aspect.
The invention discloses a correction method, a correction system and an analyzer for the liquid suction position of a reagent needle, which are used for acquiring the initial movement steps of the reagent needle and the theoretical suction amount of a reagent kit, and calculating the current theoretical movement steps of the reagent needle according to the initial movement steps and the theoretical suction amount; acquiring the current detection movement step number of the nth sample suction of the reagent needle; calculating an actual motion step number difference value between the current theoretical motion step number and the current detection motion step number; obtaining M monitoring detection movement steps according to the actual movement step difference value and a tolerable difference threshold; acquiring L limit detection motion steps based on the M monitoring detection motion steps and the actual detection motion step threshold; and acquiring the target updating theoretical movement step number based on the M monitoring detection movement step numbers or the L limit detection movement step numbers. Therefore, on the premise of not sacrificing the efficiency, the current real liquid level position of the reagent kit, namely the liquid suction position of the reagent needle, can be automatically corrected in a monitoring and judging mode, and meanwhile, the reagent needle sucks the reagent according to the current real liquid level position of the reagent kit, so that the reliability of an instrument result is ensured, and the range of abnormal testing is effectively controlled.
In order to make the above objects, features and advantages of the present invention more comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a flow chart showing a method for correcting the liquid sucking position of a reagent needle according to the present invention;
FIG. 2 is a schematic flow chart of another method for correcting the liquid sucking position of a reagent needle according to the present invention;
FIG. 3 is a schematic flow chart of another method for correcting the liquid sucking position of the reagent needle according to the present invention;
FIG. 4 is a schematic flow chart showing another method for correcting the liquid sucking position of the reagent needle according to the present invention;
fig. 5 shows a schematic structural diagram of a correction system for the liquid sucking position of the reagent needle according to the present invention.
Description of main reference numerals:
501-a kit; 502-a control device; 503-reagent needle; 504-level detection means.
Detailed Description
Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative only and are not to be construed as limiting the invention.
It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. In contrast, when an element is referred to as being "directly on" another element, there are no intervening elements present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only.
In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used in the description of the templates herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
Example 1
The embodiment of the disclosure provides a correction method for a liquid suction position of a reagent needle, which is used for detecting the liquid level position through a reagent box in a monitoring and testing process, judging whether the current liquid level position state is abnormal through comparison with a theoretical position, further judging whether a special treatment process is triggered, confirming the current reliable actual liquid level position, and further judging whether to correct the liquid suction position of the reagent needle or replace the reagent box.
Referring to fig. 1, the method for updating the number of steps of movement of the reagent needle includes steps S101 to S106, and each step is described in detail below.
Step S101, obtaining the initial movement steps of the reagent needle and the theoretical suction amount of the reagent kit, and calculating the current theoretical movement steps of the reagent needle according to the initial movement steps and the theoretical suction amount.
Specifically, determining the product of the theoretical suction amount and N-1 to obtain a target product; determining the sum value of the target product and the target coefficient to obtain a target sum value; and determining the sum value of the initial motion step number and the target sum value to obtain the current theoretical motion step number.
In the present embodiment, the initial number of exercise steps P 0 The theoretical amount of aspiration S is the amount of reagent that the kit can ideally aspirate each time, for the number of steps of movement the reagent needle needs to take for the first time from the kit. And according to the initial number of steps P 0 And the theoretical suction quantity S can be calculated to obtain the current theoretical movement step number P of the N-th test reagent needle suction sample of the kit N Number of current theoretical exercise steps P N The calculation formula of (2) is P N ＝P 0 ++ (N-1) x S+k, where k is the target coefficient.
In a specific embodiment, the obtaining the initial number of steps of movement of the reagent needle and the theoretical amount of aspiration of the reagent kit comprises: the liquid level position information is obtained when the kit is loaded; determining the initial number of movement steps according to the liquid level position information; and determining the theoretical suction amount according to the item information of the kit.
In this embodiment, during the loading of the reagent cartridge, the liquid level detection device continuously detects at a low speed for a plurality of times to obtain liquid level position information, and the control device converts the liquid level position information into an initial movement step number P of the first sample suction of the reagent needle 0 The method comprises the steps of carrying out a first treatment on the surface of the Meanwhile, the control device can identify item information of the kit, and the theoretical suction quantity S corresponding to the kit is obtained. When the liquid level detection device continuously detects the liquid level position information of the kit at a low speed, the detection can be performed in a mode of uniform speed and low speed, for example, uniform speed is 3m/s, and the acquired liquid level position information is more accurate.
Step S102, the current detection movement step number of the nth sample suction of the reagent needle is obtained.
In the present embodiment, the control means controls the reagent needle to preferentially use the current theoretical number of steps P of movement when the reagent needle sucks the reagent N The sample is sucked at a higher speed, for example 20m/s; meanwhile, when the reagent needle actually sucks a sample, the liquid level detection device synchronously detects the actual movement steps of the reagent needle at a higher speed to obtain the current detection movement steps Q N . Thus, the high speed operation will not affect the sample sucking efficiency of the reagent needle.
Step S103, calculating the difference value between the current theoretical moving step number and the actual moving step number of the current detection moving step number.
In this embodiment, the control device obtains the current theoretical number of steps P after the nth reagent is sucked by the reagent needle N With the current detected number of steps Q N And calculates the actual motion step number difference D of the two N Is used for subsequently judging whether the liquid sucking position of the current reagent needle is abnormal.
And step S104, obtaining M monitoring detection movement steps according to the actual movement step difference value and the tolerable difference threshold.
In the present embodiment, the control device can control the step number difference D by the actual movement N And a tolerable difference threshold T 1 To obtain the number of monitoring probe motion steps to determine whether the kit needs to be replaced or whether the current level of the kit needs to be corrected.
Referring to fig. 2, the step number of M monitoring detection movements is obtained according to the actual step number difference value and the tolerable difference threshold, and includes steps S1041 to S1042, which are described in detail below.
Step S1041, comparing the actual motion step difference value with a tolerable difference threshold.
In this embodiment, the control device determines the actual step number difference D N And a tolerable difference threshold T 1 And determining whether the current theoretical movement steps are consistent with the current detection movement steps according to the judgment result. Tolerance difference threshold T 1 Is based on actual practiceAnd (5) determining the test condition.
Step S1042, if the actual motion step number difference is greater than the tolerable difference threshold, continuously monitoring the sample suction of the reagent needle M times to obtain M monitoring detection motion step numbers.
In this embodiment, if the difference D of the actual number of steps is the same as the sample suction performed by the reagent needle for the nth time N Exceeding the tolerable difference threshold T 1 The difference D of the actual number of exercise steps is known N With the current detected number of steps Q N If the number of the M monitoring movement steps is not consistent with the number of the M monitoring movement steps when the control device continuously monitors the reagent needle sample suction from the nth time, the liquid level detection device synchronously acquires the M monitoring detection movement steps (Q N ～Q N+M-1 )。
In a specific embodiment, after comparing the actual motion step number difference value with the tolerable difference threshold, the method further includes: and if the actual motion step number difference value is smaller than or equal to the tolerable difference threshold value, not updating the current theoretical motion step number.
In the present embodiment, if the actual exercise step number is the difference D N Does not exceed the tolerable difference threshold T 1 And the current theoretical movement step number is consistent with the current detection movement step number, no processing is performed at the moment, and the reagent needle continues to suck samples along the current theoretical movement step number in the (n+1) th test.
Step S105, obtaining L limit detection motion steps based on the M monitoring detection motion steps and the actual detection motion step threshold.
In this embodiment, after the limit number of detection steps is obtained based on the monitoring number of detection steps and the actual number of detection steps threshold, it can be further determined whether the kit is abnormal or whether the current liquid level position of the kit, i.e., the liquid suction position of the reagent needle, needs to be corrected.
Referring to fig. 3, the step of obtaining the L limit detected motion steps based on the M monitored detected motion steps and the actual detected motion step threshold includes steps S1051 to S1053, and each step is described in detail below.
Step S1051, calculating the average value of the step numbers of the monitoring detection movement according to the M step numbers of the monitoring detection movement.
In the present embodiment, the control device acquires M monitoring detection movement steps (Q N ～Q N+M-1 ) After that, M monitoring detection movement steps (Q) N ～Q N+M-1 ) The average value of (a), namely the average value M of the number of detected motion steps 1 。
Step S1052, comparing the average value of the step number of the monitoring detection motion with the threshold value of the step number of the actual detection motion.
In this embodiment, the control device compares and monitors the detection motion step number average value M 1 And the threshold value M of the actual detection motion steps T And in combination with the comparison, determines whether the reagent needle has found a reagent level and determines whether to trigger a particular treatment procedure. Actual detection of the step number threshold M T The value range of (2) is determined according to the actual experimental condition.
And step S1053, if the average value of the step numbers of the monitoring detection movement is smaller than or equal to the threshold value of the step numbers of the actual detection movement, the reagent needle is subjected to sample suction for L times by moving to the limit position, and L limit detection movement step numbers are obtained.
In the present embodiment, if the detection motion step number average M is monitored 1 Does not exceed the threshold M of the actual detected motion steps T The control device can be used for controlling the reagent needle to move to the limit position for sucking the reagent L times when the reagent needle is in the limit sucking reagent L limit detection movement steps can be synchronously detected and obtained by the liquid level detection device. The limit position in this embodiment may be the bottom of the kit, and in other embodiments, the specific position of the limit position may be determined according to the actual requirement.
And S106, acquiring the target update theoretical movement steps based on the M monitoring detection movement steps or the L limit detection movement steps.
In this embodiment, based on the number of limit detection steps, it may be determined whether the current theoretical number of steps of the current kit needs to be corrected, and if so, the target update theoretical number of steps may also be obtained according to the number of limit detection steps.
Referring to fig. 4, the target update theoretical moving step number includes a first update theoretical moving step number, and the target update theoretical moving step number is obtained based on M monitoring detection moving step numbers or L limit detection moving step numbers, including steps S1061 to S1064, which are described in detail below.
Step S1061, calculating a mean value of the number of steps of the limit detection motion and a second variation coefficient according to the L number of steps of the limit detection motion.
In this embodiment, the control device obtains the L number of steps of the limit detection motion and calculates the average value of the L number of steps of the limit detection motion to obtain the average value M of the number of steps of the limit detection motion 2 And L second coefficient of variation CV of the limit detected motion steps 2 Second coefficient of variation CV 2 To indicate the stability of the limit detect motion steps.
In a specific embodiment, calculating the second coefficient of variation according to the L limit detection motion steps includes: and calculating the difference value of the adjacent two limit detection movement steps to obtain L-1 limit movement step difference values, and calculating the second variation coefficient according to the L-1 limit movement step difference values.
In this embodiment, first, the control device calculates the difference between every two adjacent limit detection steps, and M-1 difference E between the monitoring steps can be obtained 2 The method comprises the steps of carrying out a first treatment on the surface of the Then, adding all the limit movement step number differences, and dividing the sum by the number of the limit movement step number differences, namely L-1, to obtain an average value of the limit movement step number differences; then, calculating the standard deviation of the limit exercise step number difference value by using a calculation formula of the standard deviation; dividing the standard deviation of the difference value of the limit movement steps by the average value of the difference value of the limit movement steps, and multiplying the average value by 100% to obtain a second variation coefficient CV of the limit movement steps 2 . In other embodiments, the second coefficient of variation CV of the limit number of steps is calculated according to other methods 2 . Calculating a second coefficient of variation CV of the limit number of steps of exercise 2 Can be usedThe data determination is then performed more directly and efficiently.
Step S1062, comparing the average value of the number of steps of the limit detection motion with the threshold value of the number of steps of the actual detection motion.
In the present embodiment, the control device compares the limit detection motion step number average value M 2 And the threshold value M of the actual detection motion steps T And judging whether the current kit is available according to the comparison result.
Step S1063, comparing the second variation coefficient with a variation coefficient threshold if the average value of the number of steps of the limit detected motion is greater than the threshold of the actual number of steps of the detected motion.
In the present embodiment, if the limit detection motion step number average value M 2 Exceeding the threshold M for the number of steps of the actual detected movement T The reagent needle can find the reagent liquid level within the normal range in the special treatment process, and the control device combines the second variation coefficient CV 2 Comparing the second coefficient of variation CV 2 And coefficient of variation threshold CV T Continuing to determine if the kit is available.
In a specific embodiment, after comparing the limit detected motion step number average value with the actual detected motion step number threshold value, the method further includes: and if the average value of the limit detection movement steps is smaller than or equal to the threshold value of the actual detection movement steps, alarming that the test of the kit is abnormal, and disabling the kit.
In the present embodiment, if the limit detection motion step number average value M 2 Does not exceed the threshold M of the actual detected motion steps T The reagent box is abnormal, the control device gives an alarm and disables the reagent box, and the reagent box needs to be replaced at the moment.
Step S1064, if the second variation coefficient is less than or equal to the variation coefficient threshold, obtaining a first updated theoretical number of steps according to the average value of the number of steps of the limit detection motion.
Specifically, determining a sum value of the limit detection motion step number average value and the target sum value to obtain the first updated theoretical motion step number.
In the present embodiment, if the second variation isNumber CV 2 Coefficient of variation threshold CV is not exceeded T The control device updates the current theoretical number of the movement steps of the reagent and detects the average value M of the movement steps according to the limit 2 Acquiring a first updated theoretical movement step number P corresponding to the actual reagent liquid level in the current kit N1 The reagent needle moves according to the first updated theoretical movement step number P N1 And the reagent is continuously sucked, so that the reliability of the result is ensured. The calculation formula of the first updated theoretical movement steps is P N1 ＝M 2 ++ (N-1) x S+k, where k is the target coefficient.
In a specific embodiment, after comparing the second coefficient of variation with the coefficient of variation threshold, the method further includes: and if the second variation coefficient is larger than the variation coefficient threshold value, alarming that the test of the kit is abnormal, and disabling the kit.
In the present embodiment, if the second coefficient of variation CV 2 Exceeding coefficient of variation threshold CV T The abnormality of the kit can be known, and the control device gives an alarm and disables the kit, so that the kit needs to be replaced.
In a specific embodiment, the target updated theoretical moving step number further includes a second updated theoretical moving step number, and the obtaining the target updated theoretical moving step number based on the M monitored detected moving step numbers or the L limit detected moving step numbers further includes: if the average value of the monitoring detection motion steps is larger than the actual detection motion step number threshold value, calculating a first variation coefficient according to M monitoring detection motion steps, and comparing the first variation coefficient with the actual detection motion step number threshold value; if the first variation coefficient is smaller than or equal to the actual detection motion step number threshold value, not updating the current theoretical motion step number; and if the first variation coefficient is larger than the actual detection motion step number threshold value, obtaining a second updated theoretical motion step number according to the monitoring detection motion step number average value.
Specifically, determining the sum of the average value of the monitoring detection motion steps and the target sum value to obtain the second updated theoretical motion steps.
In the present embodiment, after step S1052, if monitoringDetecting motion step number average M 1 Greater than the threshold M of the number of actually detected motion steps T The control device can be known to find the liquid level of the reagent in the process of monitoring the reagent needle, and calculate the first variation coefficient according to the M monitoring detection steps, and combine the first variation coefficient CV 1 Judging and comparing the first coefficient of variation CV 1 And coefficient of variation threshold CV T And thus the size of the (c) is determined.
Further, if the first coefficient of variation CV 1 Coefficient of variation threshold CV is not exceeded T The detection process of the reagent needle sample suction and the liquid level detection device is considered to be stable, the control device judges that the abnormal condition leads to inaccurate current theoretical steps, the current theoretical steps need to be corrected at the moment, and the corrected second updated theoretical steps P are used subsequently N2 And controlling the suction of the reagent, and ensuring the reliability of the result. The calculation formula of the first updated theoretical movement steps is P N2 ＝M 1 ++ (N-1) x S+k, where k is the target coefficient.
Further, if the first coefficient of variation CV 1 Exceeding coefficient of variation threshold CV T And the control device continuously controls the reagent needle to absorb the reagent along the current theoretical steps without updating the current theoretical steps, and continuously monitors the reagent.
In one embodiment, calculating the first coefficient of variation from the M monitored detected motion steps includes: and calculating the difference value of the adjacent two monitoring detection movement steps to obtain M-1 monitoring movement step difference values, and calculating the first variation coefficient according to the M-1 monitoring movement step difference values.
In this embodiment, first, the control device calculates the difference between every two adjacent monitoring detection steps, and M-1 monitoring steps can be obtained 1 The method comprises the steps of carrying out a first treatment on the surface of the Then, adding all the monitoring movement step number differences, and dividing the sum by the number of the monitoring movement step number differences, namely M-1, to obtain an average value of the monitoring movement step number differences; then, calculating the standard deviation of the difference value of the monitoring exercise steps by using a calculation formula of the standard deviation; then the standard deviation for monitoring the difference value of the number of exercise stepsDividing the difference by the average value of the difference values of the monitored number of steps, and multiplying the average value by 100% to obtain a first variation coefficient CV of the monitored number of steps 1 . In other embodiments, the first coefficient of variation CV for the difference in the number of monitored steps is calculated according to other methods 1 . Calculate a first coefficient of variation CV for monitoring the difference in the number of steps of exercise 1 Can be used for the subsequent more direct and effective data judgment.
According to the correction method for the liquid suction position of the reagent needle, the initial movement steps of the reagent needle and the theoretical suction amount of the reagent kit are obtained, and the current theoretical movement steps of the reagent needle are calculated according to the initial movement steps and the theoretical suction amount; acquiring the current detection movement step number of the nth sample suction of the reagent needle; calculating an actual motion step number difference value between the current theoretical motion step number and the current detection motion step number; obtaining M monitoring detection movement steps according to the actual movement step difference value and a tolerable difference threshold; acquiring L limit detection motion steps based on the M monitoring detection motion steps and the actual detection motion step threshold; and acquiring the target updating theoretical movement step number based on the M monitoring detection movement step numbers or the L limit detection movement step numbers. Therefore, on the premise of not sacrificing the efficiency, the current real liquid level position of the reagent kit, namely the liquid suction position of the reagent needle, can be automatically corrected in a monitoring and judging mode, and meanwhile, the reagent needle sucks the reagent according to the current real liquid level position of the reagent kit, so that the reliability of an instrument result is ensured, and the range of abnormal testing is effectively controlled.
Example 2
Further, referring to fig. 5, embodiments of the present disclosure provide a system for correcting a pipetting position of a reagent needle, the system comprising:
a kit 501 for storing reagents;
a control device 502 for obtaining the initial movement steps of the reagent needle and the theoretical suction amount of the reagent kit; calculating the current theoretical movement steps of the reagent needle according to the initial movement steps and the theoretical suction amount;
a reagent needle 503, which is in communication connection with the control device and is used for sucking the sample of the reagent in the reagent kit according to the current theoretical number of movement steps;
the liquid level detection device 504 is positioned at the needle point of the reagent needle and is in communication connection with the control device, and is used for synchronously detecting the current detection movement steps of the nth sample suction of the reagent needle;
the control device 502 is further configured to obtain, from the liquid level detection device, a current detection motion step number of the nth sample suction of the reagent needle; calculating an actual motion step number difference value between the current theoretical motion step number and the current detection motion step number; obtaining M monitoring detection movement steps according to the actual movement step difference value and a tolerable difference threshold; acquiring L limit detection motion steps based on the M monitoring detection motion steps and the actual detection motion step threshold; and acquiring the target updating theoretical movement step number based on the M monitoring detection movement step numbers or the L limit detection movement step numbers.
In one embodiment, at least one of the following features (1) - (2) is included:
(1) The control device 502 is further configured to compare the actual motion step number difference value with a tolerable difference threshold; if the actual motion step number difference value is larger than the tolerable difference threshold, continuously monitoring the sample suction of the reagent needle for M times;
the liquid level detecting device 504 is further configured to synchronously detect M monitoring detection steps of the reagent needle during M sample suctions;
the control device 502 is further configured to obtain M monitoring detection motion steps from the liquid level detection device;
(2) The control device 502 is further configured to not update the current theoretical number of steps of motion if the actual number of steps of motion difference is less than or equal to the tolerable difference threshold.
In one embodiment, at least one of the following features (1) - (3) is included:
(1) The control device 502 is further configured to calculate a monitoring detection motion step number average value according to M monitoring detection motion step numbers; comparing the average value of the step number of the monitoring detection motion with the threshold value of the step number of the actual detection motion; if the average value of the step numbers of the monitoring detection motion is smaller than or equal to the threshold value of the step numbers of the actual detection motion, controlling the reagent needle to perform L times of sample suction by moving to the limit position;
The liquid level detection device 504 is further configured to synchronously detect L limit detection steps of the reagent needle during L times of sample suction;
the control device 502 is further configured to obtain L limit detection motion steps;
the target updated theoretical number of steps includes a first updated theoretical number of steps, and the control device 502 is further configured to calculate a mean value of the number of steps of the limit detection motion and a second coefficient of variation according to the L number of steps of the limit detection motion, respectively; comparing the average value of the limit detection motion steps with an actual detection motion step threshold value; if the average value of the limit detection motion steps is larger than the threshold value of the actual detection motion steps, comparing the second variation coefficient with the threshold value of the variation coefficient; if the second variation coefficient is smaller than or equal to the variation coefficient threshold value, obtaining the first updated theoretical number of motion steps according to the limit detection motion step number average value;
the reagent needle 503 is further configured to perform sample suction according to the first updated theoretical number of exercise steps;
the control device 502 is further configured to determine a sum of the average value of the number of steps of the limit detection motion and the target sum value, to obtain the first updated theoretical number of steps of motion; the step of calculating the second coefficient of variation from the L limit detection motion steps comprises: calculating the difference value of two adjacent limit detection motion steps to obtain L-1 limit motion step difference values, and calculating the second variation coefficient according to the L-1 limit motion step difference values;
(2) The control device 502 is further configured to alarm the kit that the test is abnormal and disable the kit if the second coefficient of variation is greater than the coefficient of variation threshold;
(3) The control device 502 is further configured to alarm that the test of the kit is abnormal and disable the kit if the average value of the number of steps of the limit detection motion is less than or equal to the threshold value of the number of steps of the actual detection motion.
In an embodiment, the target updated theoretical number of steps of motion further includes a second updated theoretical number of steps of motion, and the control device 502 is further configured to calculate a first variation coefficient according to M monitored detected steps of motion if the average value of the monitored detected steps of motion is greater than the threshold value of the actual detected steps of motion, and compare the first variation coefficient with the threshold value of the actual detected steps of motion; if the first variation coefficient is smaller than or equal to the actual detection motion step number threshold value, not updating the current theoretical motion step number; if the first variation coefficient is larger than the actual detection motion step number threshold value, obtaining a second updated theoretical motion step number according to the monitoring detection motion step number average value; determining the sum value of the average value of the monitoring detection motion steps and the target sum value to obtain the second updated theoretical motion steps;
The reagent needle 503 is further used for sucking samples from the reagent kit according to the second updated theoretical number of exercise steps;
the control device 502 is further configured to calculate a difference between two adjacent monitored motion steps to obtain M-1 monitored motion steps, and calculate a first coefficient of variation according to the M-1 monitored motion steps.
The system provided in the embodiments of the present disclosure may perform the steps of the method for correcting the liquid sucking position of the reagent needle provided in embodiment 1, and in order to avoid repetition, a description is omitted.
The correction system for the liquid suction position of the reagent needle provided by the embodiment obtains the initial movement step number of the reagent needle and the theoretical suction amount of the reagent kit, and calculates the current theoretical movement step number of the reagent needle according to the initial movement step number and the theoretical suction amount; acquiring the current detection movement step number of the nth sample suction of the reagent needle; calculating an actual motion step number difference value between the current theoretical motion step number and the current detection motion step number; obtaining M monitoring detection movement steps according to the actual movement step difference value and a tolerable difference threshold; acquiring L limit detection motion steps based on the M monitoring detection motion steps and the actual detection motion step threshold; and acquiring the target updating theoretical movement step number based on the M monitoring detection movement step numbers or the L limit detection movement step numbers. Therefore, on the premise of not sacrificing the efficiency, the current real liquid level position of the reagent kit, namely the liquid suction position of the reagent needle, can be automatically corrected in a monitoring and judging mode, and meanwhile, the reagent needle sucks the reagent according to the current real liquid level position of the reagent kit, so that the reliability of an instrument result is ensured, and the range of abnormal testing is effectively controlled.
Example 3
The invention provides an analyzer, which comprises the correction system of the liquid sucking position of the reagent needle in the embodiment 2, and is not repeated for avoiding repetition.
The analyzer may be an electrochemiluminescence analyzer, a specific protein analyzer, a glycosylated hemoglobin analyzer, or the like, and the present embodiment is not limited thereto.
Any particular values in all examples shown and described herein are to be construed as merely illustrative and not a limitation, and thus other examples of exemplary embodiments may have different values.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
The above examples merely represent a few embodiments of the present invention, which are described in more detail and are not to be construed as limiting the scope of the present invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention.
Claims (16)
1. A method of correcting a fluid intake position of a reagent needle, the method comprising:
acquiring the initial movement step number of a reagent needle and the theoretical suction quantity of a kit, and calculating the current theoretical movement step number of the reagent needle according to the initial movement step number and the theoretical suction quantity;
acquiring the current detection movement step number of the nth sample suction of the reagent needle;
calculating an actual motion step number difference value between the current theoretical motion step number and the current detection motion step number;
obtaining M monitoring detection movement steps according to the actual movement step difference value and a tolerable difference threshold;
acquiring L limit detection motion steps based on the M monitoring detection motion steps and the actual detection motion step threshold;
and acquiring the target updating theoretical movement step number based on the M monitoring detection movement step numbers or the L limit detection movement step numbers.
2. The method of claim 1, wherein the step of obtaining M monitoring probe motion steps from the actual motion step difference and a tolerable difference threshold comprises:
comparing the actual motion step number difference value with a tolerable difference threshold;
and if the actual movement step number difference value is larger than the tolerable difference threshold, continuously monitoring the sample suction of the reagent needle for M times to obtain M monitoring detection movement step numbers.
3. The method of correcting a reagent needle pipetting position according to any one of claims 1 to 2, wherein the target updated theoretical number of steps of movement includes a first updated theoretical number of steps of movement, and the obtaining the target updated theoretical number of steps of movement based on the M monitored detected number of steps of movement or the L limit detected number of steps of movement includes:
calculating a limit detection motion step number average value and a second variation coefficient according to the L limit detection motion step numbers;
comparing the average value of the limit detection motion steps with the threshold value of the actual detection motion steps;
if the average value of the limit detection motion steps is larger than the threshold value of the actual detection motion steps, comparing the second variation coefficient with the threshold value of the variation coefficient;
and if the second variation coefficient is smaller than or equal to the variation coefficient threshold value, obtaining the first updated theoretical number of motion steps according to the limit detection motion step number average value.
4. The method of correcting a reagent needle pipetting position according to any one of claims 1-2, wherein the obtaining L limit detection movement steps based on M of the monitor detection movement steps and an actual detection movement step threshold value comprises:
Calculating the average value of the step numbers of the monitoring detection motion according to the M step numbers of the monitoring detection motion;
comparing the average value of the step number of the monitoring detection motion with the threshold value of the step number of the actual detection motion;
if the average value of the monitoring detection movement steps is smaller than or equal to the threshold value of the actual detection movement steps, the reagent needle is subjected to L times of sample suction by moving to the limit position, and L limit detection movement steps are obtained.
5. The method of correcting a reagent needle pipetting position as recited in claim 4, wherein the target updated theoretical number of steps of movement further includes a second updated theoretical number of steps of movement, the target updated theoretical number of steps of movement being obtained based on the M monitored detected number of steps of movement or the L limit detected number of steps of movement, further comprising:
if the average value of the monitoring detection motion steps is larger than the actual detection motion step number threshold value, calculating a first variation coefficient according to M monitoring detection motion steps, and comparing the first variation coefficient with the actual detection motion step number threshold value;
if the first variation coefficient is smaller than or equal to the actual detection motion step number threshold value, not updating the current theoretical motion step number;
And if the first variation coefficient is larger than the actual detection motion step number threshold value, obtaining the second updated theoretical motion step number according to the monitoring detection motion step number average value.
6. The method of correcting a reagent needle pipetting position as recited in claim 5, comprising at least one of the following features (1) - (3):
(1) Said calculating a current theoretical number of steps of movement of said reagent needle based on said initial number of steps of movement and said theoretical amount of aspiration, comprising:
determining the product of the theoretical suction quantity and N-1 to obtain a target product;
determining the sum value of the target product and the target coefficient to obtain a target sum value;
determining the sum value of the initial motion step number and the target sum value to obtain the current theoretical motion step number;
(2) The step number average value of the motion steps according to the limit detection is used for obtaining a first updated theoretical motion step number, and the step number average value comprises the following steps: determining the sum value of the limit detection motion step number average value and the target sum value to obtain the first updated theoretical motion step number;
(3) The step number obtaining a second updated theoretical step number according to the average value of the step numbers of the monitoring detection motion comprises the following steps: and determining the sum value of the average value of the monitoring detection motion steps and the target sum value to obtain the second updated theoretical motion steps.
7. The method of correcting a reagent needle pipetting position as recited in claim 5, wherein the step of calculating a first coefficient of variation from the number of M of the monitor probe motion steps comprises: and calculating the difference value of the adjacent two monitoring detection movement steps to obtain M-1 monitoring movement step difference values, and calculating the first variation coefficient according to the M-1 monitoring movement step difference values.
8. The method for correcting a reagent needle pipetting position according to any one of claims 1-2, wherein the obtaining of the initial number of steps of movement of the reagent needle and the theoretical pipetting amount of the reagent cartridge comprises:
the liquid level position information is obtained when the kit is loaded;
determining the initial number of movement steps according to the liquid level position information;
and determining the theoretical suction amount according to the item information of the kit.
9. A method of correcting a reagent needle pipetting position as recited in claim 3, comprising at least one of the following features (1) - (3):
(1) The step of calculating the second coefficient of variation from the L limit detection motion steps includes: calculating the difference value of two adjacent limit detection motion steps to obtain L-1 limit motion step difference values, and calculating the second variation coefficient according to the L-1 limit motion step difference values;
(2) After comparing the limit detection motion step number average value with the actual detection motion step number threshold value, the method further comprises the following steps: if the average value of the number of the limit detection movement steps is smaller than or equal to the threshold value of the actual detection movement steps, alarming that the test of the kit is abnormal, and disabling the kit;
(3) After comparing the second coefficient of variation with the coefficient of variation threshold, the method further comprises: and if the second variation coefficient is larger than the variation coefficient threshold value, alarming that the test of the kit is abnormal, and disabling the kit.
10. The method of correcting a reagent needle pipetting position as recited in claim 2, wherein the comparing the actual number of steps of movement difference to a tolerable difference threshold further comprises: and if the actual motion step number difference value is smaller than or equal to the tolerable difference threshold value, not updating the current theoretical motion step number.
11. A system for correcting a fluid-aspirating position of a reagent needle, the system comprising:
a kit for storing reagents;
the control device is used for acquiring the initial movement steps of the reagent needle and the theoretical suction quantity of the reagent kit; calculating the current theoretical movement steps of the reagent needle according to the initial movement steps and the theoretical suction amount;
The reagent needle is in communication connection with the control device and is used for sucking the reagent in the kit according to the current theoretical number of movement steps;
the liquid level detection device is positioned at the needle point of the reagent needle, is in communication connection with the control device and is used for synchronously detecting the current detection movement steps of the nth sample suction of the reagent needle;
the control device is also used for acquiring the current detection movement steps of the nth sample suction of the reagent needle from the liquid level detection device; calculating an actual motion step number difference value between the current theoretical motion step number and the current detection motion step number; obtaining M monitoring detection movement steps according to the actual movement step difference value and a tolerable difference threshold; acquiring L limit detection motion steps based on the M monitoring detection motion steps and the actual detection motion step threshold; and acquiring the target updating theoretical movement step number based on the M monitoring detection movement step numbers or the L limit detection movement step numbers.
12. The system for correcting the pipetting position of a reagent needle as recited in claim 11 wherein the level detection device is further configured to obtain level information when the reagent cartridge is loaded and transmit the level information to the control device;
The control device is also used for determining the initial movement step number according to the liquid level position information; determining the theoretical suction amount according to item information of the kit; determining the product of the theoretical suction quantity and N-1 to obtain a target product; determining the sum value of the target product and the target coefficient to obtain a target sum value; and determining the sum value of the initial motion step number and the target sum value to obtain the current theoretical motion step number.
13. The system for correcting a reagent needle pipetting position as recited in claim 11, comprising at least one of the following features (1) - (2):
(1) The control device is also used for comparing the actual motion step number difference value with a tolerable difference threshold; if the actual motion step number difference value is larger than the tolerable difference threshold, continuously monitoring the sample suction of the reagent needle for M times;
the liquid level detection device is also used for synchronously detecting M monitoring detection movement steps of the reagent needle during M times of sample suction;
the control device is also used for acquiring M monitoring detection movement steps from the liquid level detection device;
(2) The control device is further configured to not update the current theoretical number of steps of motion if the actual number of steps of motion difference is less than or equal to the tolerable difference threshold.
14. A correction system for the fluid intake position of a reagent needle according to any one of claims 12 to 13, characterized by comprising at least one of the following features (1) - (3):
(1) The control device is also used for calculating the average value of the monitoring detection motion steps according to the M monitoring detection motion steps; comparing the average value of the step number of the monitoring detection motion with the threshold value of the step number of the actual detection motion; if the average value of the step numbers of the monitoring detection motion is smaller than or equal to the threshold value of the step numbers of the actual detection motion, controlling the reagent needle to perform L times of sample suction by moving to the limit position;
the liquid level detection device is also used for synchronously detecting L limit detection movement steps of the reagent needle during L times of sample suction;
the control device is also used for acquiring L limit detection motion steps;
the target updating theoretical movement step number comprises a first updating theoretical movement step number, and the control device is further used for respectively calculating a limit detection movement step number average value and a second variation coefficient according to the L limit detection movement step numbers; comparing the average value of the limit detection motion steps with an actual detection motion step threshold value; if the average value of the limit detection motion steps is larger than the threshold value of the actual detection motion steps, comparing the second variation coefficient with the threshold value of the variation coefficient; if the second variation coefficient is smaller than or equal to the variation coefficient threshold value, obtaining the first updated theoretical number of motion steps according to the limit detection motion step number average value;
The reagent needle is also used for sucking samples according to the first updated theoretical movement steps;
the control device is further used for determining the sum value of the limit detection motion step number average value and the target sum value to obtain the first updated theoretical motion step number; the step of calculating the second coefficient of variation from the L limit detection motion steps comprises: calculating the difference value of two adjacent limit detection motion steps to obtain L-1 limit motion step difference values, and calculating the second variation coefficient according to the L-1 limit motion step difference values;
(2) The control device is further used for alarming that the kit is abnormal in test and disabling the kit if the second variation coefficient is larger than the variation coefficient threshold;
(3) And the control device is also used for alarming the test abnormality of the kit and disabling the kit if the average value of the number of steps of the limit detection motion is smaller than or equal to the threshold value of the number of steps of the actual detection motion.
15. The system for correcting a reagent needle pipetting position as recited in claim 14, wherein the target updated theoretical number of steps of movement further comprises a second updated theoretical number of steps of movement, and wherein the control device is further configured to calculate a first coefficient of variation from M monitored detected steps of movement if the monitored detected steps of movement average is greater than the actual detected steps of movement threshold, and compare the first coefficient of variation to the actual detected steps of movement threshold; if the first variation coefficient is smaller than or equal to the actual detection motion step number threshold value, not updating the current theoretical motion step number; if the first variation coefficient is larger than the actual detection motion step number threshold value, obtaining a second updated theoretical motion step number according to the monitoring detection motion step number average value; determining the sum value of the average value of the monitoring detection motion steps and the target sum value to obtain the second updated theoretical motion steps;
The reagent needle is also used for sucking samples from the reagent kit according to the second updated theoretical number of movement steps;
the control device is also used for calculating the difference value of the adjacent two monitoring detection movement steps to obtain M-1 monitoring movement step difference values, and calculating a first variation coefficient according to the M-1 monitoring movement step difference values.
16. An analyser comprising a correction system for the fluid-intake position of a reagent needle according to any one of claims 11 to 15.
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