CN117825639B - Screening method of synergistic auxiliary agent of thrips prevention and treatment medicine - Google Patents
Screening method of synergistic auxiliary agent of thrips prevention and treatment medicine Download PDFInfo
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Abstract
The invention belongs to the technical field of pesticide auxiliary agents, and particularly relates to a screening method of a synergistic auxiliary agent of a thrips prevention and treatment drug. According to the screening method of the synergistic auxiliary agent of the thrips prevention and treatment medicine, provided by the invention, through analyzing a toxicity equation and LC50 of a synergistic auxiliary agent solution formed by mixing sesamin and fast T with different proportions and a thrips prevention and treatment medicine mixed solution, the effect of the sesamin on killing thrips can be improved, and the optimal formula of the synergistic auxiliary agent is screened.
Description
Technical Field
The invention belongs to the technical field of pesticide auxiliaries, and particularly relates to a screening method of a synergistic auxiliary of a thrips prevention and treatment drug.
Background
The thrips are small in size, fast in propagation speed, fast in iteration, hidden in activity, easy to generate resistance to the control agent, effective in controlling agent deficiency, and thrips are ovisioned in plant tissues, so that all or most of insect states of the thrips are difficult to kill by 1 agent or 1-time spraying, and the insect pests are called as 'resistant insects' in agricultural production. The damage of resistant small insects thrips is increasingly aggravated in recent years, and the resistant small insects thrips become the first difficult problem in the planting of fruit and vegetable crops in China. The dosage is frequently increased in many areas, and the application times are increased, so that the resistance of thrips to a plurality of medicaments is enhanced, and the application effect is not ideal.
Sesamin can enhance the deinsectization effect of the insecticide in agriculture, has synergistic effect on thrips prevention and treatment medicines, is a natural component hidden in sesame seeds, has very little content and higher extraction cost, and therefore, the additive content of sesamin in the thrips prevention and treatment medicines needs to be screened to reduce the cost of the thrips prevention and treatment medicines.
Disclosure of Invention
The invention aims to provide a screening method of a synergistic agent of a thrips prevention and treatment drug, which aims to solve the problems in the background technology.
In order to achieve the technical purpose, the technical scheme of the invention comprises the following steps:
the screening method of the synergistic auxiliary agent of the thrips prevention and treatment medicine is characterized by comprising the following steps of:
S1: determining a toxicity equation and LC50 of the thrips prevention and treatment drug on killing thrips by adopting a leaf dipping method;
S2: compounding the fast T and sesamin with different concentrations to obtain synergistic auxiliary solution with different concentrations, and respectively measuring a toxicity equation and LC50 of a mixed solution of 98% thrips prevention and treatment medicine and 2% synergistic auxiliary solution on killing thrips by adopting a leaf dipping method;
S3: and (3) selecting the synergistic auxiliary solution with the optimal proportion according to the toxicity equation and the LC50 measured in the step S2.
As an improvement, the leaf dipping method comprises the following specific steps:
s1: taking 5 parts of thrips control drugs or mixed liquid of thrips control drugs and synergistic additives, respectively diluting the mixed liquid into 5 concentration gradient experimental liquid medicines of 500 mug/mL, 400 mug/mL, 300 mug/mL, 200 mug/mL and 100 mug/mL, wherein the numbers are respectively 1,2,3, 4 and 5, pure water is used as a control group, and the number is 6;
S2: soaking six clean and insect-free tomato leaves in No. 1, no. 2, no. 3, no. 4, no. 5 and No. 6 liquid medicine respectively, taking out after 30s, placing the tomato leaves in a plate with moisture-preserving filter paper, naturally airing the rear surfaces of the tomato leaves upwards to form No. 1, no. 2, no. 3, no. 4, no. 5 and No. 6 aired tomato leaves;
S3: the method comprises the steps of (1), 2, 3, 4, 5 and 6) respectively inserting the back surfaces of sun-dried tomato leaves into 50 adult thrips through a soft brush, sealing a plate with a preservative film, perforating the preservative film with a 0 insect needle, respectively spraying the experimental liquid in the step (1), putting the plate in a climatic chamber with the temperature of 25+/-1 ℃ and the humidity of 60+/-5% for feeding for 14 hours by illumination every day, taking out, observing and counting the survival condition of the adult thrips after 24 hours, lightly touching the adult thrips through the soft brush during observation, and judging that the thrips die after 2 times of immobility;
S4: repeating the steps S1-S3 3 times, and correcting the measurement result to obtain a toxicity equation and LC50 of the thrips control drug or the mixed solution of the thrips control drug and the synergistic agent on the thrips killing effect.
As an improvement, the thrips prevention and treatment drug is one of 5% acetamiprid, 2% chlorfenapyr or 2% deltamethrin.
As a further improvement, the 5% acetamiprid comprises the following components in percentage by weight: 5% of acetamiprid, 15% of methanol, 30% of ethanol, 10% of DMF, 5% of polyether nonionic surfactant and 35% of deionized water.
As a further improvement, the 2% chlorfenapyr comprises the following components in percentage by weight: 2% of chlorfenapyr, 2% of missible oil, 10% of DMF, 5% of Tween 80, 5% of ethanol and 76% of dimethylbenzene.
As a further improvement, the 2% deltamethrin comprises the following components in percentage by weight: 2% of deltamethrin, 2% of emulsifiable concentrate, 5% of ethanol, 5% of 2201 surfactant and 86% of dimethylbenzene.
As a further improvement, the sesamin extraction method comprises the following steps:
S1: taking a certain amount of sesame seeds, crushing the sesame seeds, adding methanol which is 8 times the weight of the sesame seeds, stirring for 30min, standing for 2 hours, and layering to form supernatant and lower-layer residues;
s2: adding methanol which is 4 times the weight of the lower layer residue into the lower layer residue in the step S1, stirring for 30min, standing for 2 hours, and layering to form supernatant and lower layer residue;
S3: mixing the supernatant in the step S2 with the supernatant in the step S1 to form a sesamin crude product;
s4: distilling the sesamin crude product under reduced pressure to form sesamin oily matter;
S5: adding methanol with the same weight as the sesamin oily matter into the sesamin oily matter, stirring for 30min, standing for 2 hours for layering, and taking supernatant to obtain sesamin.
As a further improvement, in the step S4, the vacuum degree of reduced pressure distillation is 0.06-0.09 MPa, the temperature is 75-90 ℃, and the time of reduced pressure distillation is 50-70 min.
As a further improvement, in step S2, the mass ratio of the fast T to the sesamin in the synergistic agent solution is 99 to 99.9:0.1 to 1.
By adopting the technical scheme, the invention has the beneficial effects that:
according to the screening method of the synergistic auxiliary agent of the thrips prevention and treatment medicine, provided by the invention, the virulence equation and the LC50 of sesamin and fast T and three thrips prevention and treatment medicine mixed solutions with different concentrations are respectively measured, and the optimal formula of the synergistic auxiliary agent solution can be screened out through analysis of the virulence equation and the LC50, and is mixed with the corresponding thrips prevention and treatment medicine, so that the thrips prevention and treatment effect is improved.
Detailed Description
The technical solution of the present invention will be clearly and completely described in conjunction with the specific embodiments, but it will be understood by those skilled in the art that the examples described below are some, but not all, examples of the present invention, and are intended to be illustrative only and should not be construed as limiting the scope of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
In the embodiment 1-embodiment 2, the thrips prevention and treatment drug is 5% acetamiprid, and the 5% acetamiprid comprises the following components in percentage by mass: 5% of acetamiprid, 15% of methanol, 30% of ethanol, 10% of DMF (dimethyl formamide), 5% of polyether nonionic surfactant and 35% of deionized water.
Example 1
And determining a toxicity equation and LC50 of the killing effect of 5% acetamiprid on thrips by adopting a leaf dipping method.
The leaf dipping method comprises the following specific steps:
S1: taking 5 parts of thrips prevention and treatment medicines, respectively diluting the medicines into 5 concentration gradient experimental liquid medicines of 500 mug/mL, 400 mug/mL, 300 mug/mL, 200 mug/mL and 100 mug/mL, wherein the numbers are respectively 1, 2, 3, 4 and 5, pure water is used as a control group, and the number is 6;
S2: soaking six clean and insect-free tomato leaves in No. 1, no. 2, no. 3, no. 4, no. 5 and No. 6 liquid medicine respectively, taking out after 30s, placing the tomato leaves in a plate with moisture-preserving filter paper, naturally airing the rear surfaces of the tomato leaves upwards to form No. 1, no. 2, no. 3, no. 4, no. 5 and No. 6 aired tomato leaves;
S3: the method comprises the steps of (1), 2, 3, 4, 5 and 6) respectively inserting the back surfaces of sun-dried tomato leaves into 50 adult thrips through a soft brush, sealing a plate with a preservative film, perforating the preservative film with a 0 insect needle, respectively spraying the experimental liquid in the step (1), putting the plate in a climatic chamber with the temperature of 25+/-1 ℃ and the humidity of 60+/-5% for feeding for 14 hours by illumination every day, taking out, observing and counting the survival condition of the adult thrips after 24 hours, lightly touching the adult thrips through the soft brush during observation, and judging that the thrips die after 2 times of immobility;
S4: repeating the steps S1-S3 3 times, and correcting the measurement result to obtain a virulence equation and LC50 of the killing effect of the 5% acetamiprid on the thrips.
The virulence equation and LC50 of 5% acetamiprid to thrips killing effect are shown in table 1:
TABLE 1
Example 2
Sesamin and kuai T are compounded into synergistic auxiliary agent solutions with different concentrations, and the compounding ratio is as shown in table 2:
TABLE 2
The toxicity equation and LC50 of the killing effect of 98% of mixed solution of 5% acetamiprid and 2% H1, 98% of mixed solution of 5% acetamiprid and 2% H2, 98% of mixed solution of 5% acetamiprid and 2% H3, 98% of mixed solution of 5% acetamiprid and 2% H4, 98% of mixed solution of 5% acetamiprid and 2% H5, 98% of mixed solution of 5% acetamiprid and 2% H6 on thrips are respectively measured by adopting a leaf dipping method, the specific steps of the leaf dipping method are the same as those of example 1, and experimental results are shown in Table 3:
TABLE 3 Table 3
As can be seen from Table 3, the synergistic adjuvant solution formed by compounding 0.1% sesamin and 99.9% fast T has the best synergistic effect on 5% acetamiprid.
In this example, sesamin is prepared as follows:
S1: taking a certain amount of sesame seeds, crushing the sesame seeds, adding methanol which is 8 times the weight of the sesame seeds, stirring for 30min, standing for 2 hours, and layering to form supernatant and lower-layer residues;
s2: adding methanol which is 4 times the weight of the lower layer residue into the lower layer residue in the step S1, stirring for 30min, standing for 2 hours, and layering to form supernatant and lower layer residue;
s3: mixing the supernatant in the step S2 with the supernatant in the step S1 to form a sesamin crude product;
s4: carrying out reduced pressure distillation on the sesamin crude product in the step S3 to form sesamin oily matter, wherein the vacuum degree of reduced pressure distillation is 0.06-0.09 MPa, the temperature is 75-90 ℃, and the time of reduced pressure distillation is 50-70 min;
s5: adding methanol with the same weight as the sesamin oily matter into the sesamin oily matter in the step S4, stirring for 30min, standing for 2 hours for layering, and taking supernatant to obtain sesamin.
In the examples 3 and 4, the thrips prevention and treatment medicine is 2% chlorfenapyr, and the 2% chlorfenapyr comprises the following components in percentage by mass: 2% of chlorfenapyr, 2% of missible oil, 10% of DMF, 5% of Tween 80, 5% of ethanol and 76% of dimethylbenzene.
Example 3
The toxicity equation and LC50 of the effect of 2% chlorfenapyr on thrips were determined by the leaf dipping method, the specific steps of the leaf dipping method are the same as those of example 1, and the toxicity equation and LC50 of the effect of 2% chlorfenapyr on thrips are shown in table 4:
TABLE 4 Table 4
Example 4
Sesamin and fast T are compounded into synergistic assistant solutions with different concentrations, and the compounding ratio is the same as that of example 2.
The toxicity equation and LC50 of the killing effect of 98% of 2% chlorfenapyr and 2% H1 mixed solution, 98% of 2% chlorfenapyr and 2% H2 mixed solution, 98% of 2% chlorfenapyr and 2% H3 mixed solution, 98% of 2% chlorfenapyr and 2% H4 mixed solution, 98% of 2% chlorfenapyr and 2% H5 mixed solution, 98% of 2% chlorfenapyr and 2% H6 mixed solution on thrips are respectively measured by adopting a leaf dipping method, the specific steps of the leaf dipping method are the same as those of example 1, and the experimental results are shown in table 5:
TABLE 5
As can be seen from Table 5, the synergistic adjuvant solution formed by compounding 0.6% sesamin and 99.4% fast T has the best synergistic effect on 2% chlorfenapyr.
In this example, sesamin was prepared in the same manner as in example 2.
In examples 5 and 6, the thrips control drug is 2% deltamethrin, and the 2% deltamethrin comprises the following components in percentage by mass: 2% of deltamethrin, 2% of emulsifiable concentrate, 5% of ethanol, 5% of 2201 surfactant and 86% of dimethylbenzene.
Example 5
The toxicity equation and LC50 of the killing effect of 2% deltamethrin on thrips were determined by the leaf dipping method, and the specific steps of the leaf dipping method are the same as those of example 1, and the toxicity equation and LC50 of the killing effect of 2% deltamethrin on thrips in this example are shown in table 6:
TABLE 6
Example 6
Sesamin and fast T are compounded into synergistic assistant solutions with different concentrations, and the compounding ratio is the same as that of example 2.
The toxicity equation and LC50 of the killing effect of 98% of the mixed solution of 4.5% deltamethrin and 2% of h1, 98% of the mixed solution of 4.5% deltamethrin and 2% of h2, 98% of the mixed solution of 4.5% deltamethrin and 2% of h3, 98% of the mixed solution of 4.5% deltamethrin and 2% of h4, 98% of the mixed solution of 4.5% deltamethrin and 2% of h5, 98% of the mixed solution of 4.5% deltamethrin and 2% of h6 on thrips were respectively measured by a leaf dipping method, and the specific steps of the leaf dipping method are the same as those of example 1, and the experimental results are shown in table 7:
TABLE 7
As can be seen from Table 7, the synergistic adjuvant solution formed by compounding 0.4% sesamin and 99.6% fast T has the best synergistic effect on 4.5% deltamethrin.
In this example, sesamin was prepared in the same manner as in example 2.
In examples 1-6 of the present invention, fast T is fast penetrant T, and its chemical name is diisooctyl sodium sulfosuccinate.
The above-described embodiments of the present invention do not limit the scope of the present invention. Any other corresponding changes and modifications made in accordance with the technical idea of the present invention shall be included in the scope of the claims of the present invention.
Claims (5)
1. The screening method of the synergistic auxiliary agent of the thrips prevention and treatment medicine is characterized by comprising the following steps of:
S1: determining a toxicity equation and LC50 of the thrips prevention and treatment drug on killing thrips by adopting a leaf dipping method;
S2: compounding the fast T and sesamin with different concentrations to obtain synergistic auxiliary solution with different concentrations, and respectively measuring a toxicity equation and LC50 of a mixed solution of 98% thrips prevention and treatment medicine and 2% synergistic auxiliary solution on killing thrips by adopting a leaf dipping method;
S3: selecting a synergistic auxiliary solution with the optimal proportion according to the toxicity equation and the LC50 measured in the step S2;
Wherein the thrips prevention and treatment medicine is one of 5% acetamiprid, 2% chlorfenapyr or 2% deltamethrin;
The 5% acetamiprid comprises the following components in percentage by weight: 5% of acetamiprid, 15% of methanol, 30% of ethanol, 10% of DMF, 5% of polyether nonionic surfactant and 35% of deionized water;
the 2% chlorfenapyr comprises the following components in percentage by weight: 2% of chlorfenapyr, 2% of emulsifiable concentrate, 10% of DMF, 805% of Tween, 5% of ethanol and 76% of dimethylbenzene;
the 2% deltamethrin comprises the following components in percentage by weight: 2% of deltamethrin, 2% of emulsifiable concentrate, 5% of ethanol, 5% of 2201 surfactant and 86% of dimethylbenzene.
2. The screening method of synergistic auxiliary agent of thrips prevention and treatment medicine according to claim 1, wherein the leaf dipping method comprises the following specific steps:
s1: taking 5 parts of thrips control drugs or mixed liquid of thrips control drugs and synergistic additives, respectively diluting the mixed liquid into 5 concentration gradient experimental liquid medicines of 500 mug/mL, 400 mug/mL, 300 mug/mL, 200 mug/mL and 100 mug/mL, wherein the numbers are respectively 1,2,3, 4 and 5, pure water is used as a control group, and the number is 6;
S2: soaking six clean and insect-free tomato leaves in No. 1, no. 2, no. 3, no. 4, no. 5 and No. 6 liquid medicine respectively, taking out after 30 seconds, placing the tomato leaves in a plate with moisture-preserving filter paper, naturally airing the rear surfaces of the tomato leaves upwards to form No. 1, no. 2, no. 3, no. 4, no. 5 and No. 6 aired tomato leaves;
S3: the method comprises the steps of (1), 2, 3, 4, 5 and 6) respectively inserting the back surfaces of sun-dried tomato leaves into 50 adult thrips through a soft brush, sealing a plate with a preservative film, perforating the preservative film with a 0 insect needle, respectively spraying the experimental liquid in the step (1), putting the plate in a climatic chamber with the temperature of 25+/-1 ℃ and the humidity of 60+/-5% for feeding for 14 hours by illumination every day, taking out, observing and counting the survival condition of the adult thrips after 24 hours, lightly touching the adult thrips through the soft brush during observation, and judging that the thrips die after 2 times of immobility;
S4: repeating the steps S1-S3 3 times, and correcting the measurement result to obtain a toxicity equation and LC50 of the thrips control drug or the mixed solution of the thrips control drug and the synergistic agent on the thrips killing effect.
3. The screening method of the synergistic adjuvant of the thrips prevention and treatment medicine according to claim 1, wherein the extraction method of sesamin comprises the following steps:
S1: taking a certain amount of sesame seeds, crushing the sesame seeds, adding methanol which is 8 times the weight of the sesame seeds, stirring for 30min, standing for 2 hours, and layering to form supernatant and lower-layer residues;
s2: adding methanol which is 4 times the weight of the lower layer residue into the lower layer residue in the step S1, stirring for 30min, standing for 2 hours, and layering to form supernatant and lower layer residue;
S3: mixing the supernatant in the step S2 with the supernatant in the step S1 to form a sesamin crude product;
s4: distilling the sesamin crude product under reduced pressure to form sesamin oily matter;
S5: adding methanol with the same weight as the sesamin oily matter into the sesamin oily matter, stirring for 30min, standing for 2 hours for layering, and taking supernatant to obtain sesamin.
4. The screening method of the synergistic agent of the thrips prevention and treatment medicine according to claim 3, wherein in the step S4, the vacuum degree of reduced pressure distillation is 0.06-0.09 MPa, the temperature is 75-90 ℃, and the time of reduced pressure distillation is 50-70 min.
5. The screening method of the synergistic agent of the thrips prevention and treatment medicine according to claim 1, wherein in the step S2, the mass ratio of the fast T to the sesamin in the synergistic agent solution is 99-99.9: 0.1 to 1.
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| CN101092421A (en) * | 2006-06-20 | 2007-12-26 | 白心亮 | New technique for extracting sesamin |
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