CN117825242A - Method and device for detecting blood sample, equipment and storage medium - Google Patents
Method and device for detecting blood sample, equipment and storage medium Download PDFInfo
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- 210000004369 blood Anatomy 0.000 title claims abstract description 127
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The embodiment of the invention discloses a method and a device for detecting a blood sample, equipment and a storage medium, wherein the method comprises the following steps: acquiring a cell volume distribution diagram of a blood sample to be detected and a detection type of the blood sample to be detected; extracting position information of a wave crest, a starting point and an end point of the wave on the cell volume distribution map; calculating according to the position information of the wave crest, the starting point and the end point to obtain the peak width ratio of the cell volume distribution map; and comparing the detection type, the peak width ratio and the position information of the wave crest with a preset waveform characteristic threshold value to determine whether the blood sample to be detected is abnormal. According to the scheme, parameters for judgment are obtained according to the cell volume distribution diagram, the parameters are compared with the preset waveform characteristic threshold according to the type of the problem to be detected, whether the blood sample to be detected is abnormal or not is judged, and the problem that serious loss is caused to hospitals and patients due to the fact that the final blood analysis result is distorted is solved by timely finding out the abnormal blood sample.
Description
Technical Field
The present invention relates to the field of blood analysis technologies, and in particular, to a blood sample-based detection method and apparatus, a device, and a storage medium.
Background
When a blood analyzer is used to detect a blood sample, there may be a large error in the detection result due to many factors, and if the analysis is continued on the basis of the detection result with a large error, the final blood analysis result may be distorted, which may cause serious loss to hospitals and patients.
Disclosure of Invention
In view of the foregoing, it is necessary to propose a method and apparatus for detecting a blood sample, a device and a storage medium for realizing effective reduction of probability of distortion of blood analysis results.
To achieve the above object, a first aspect of the present application provides a method for detecting a blood sample, the method comprising:
obtaining a cell volume distribution diagram of a blood sample to be detected and a detection type of the blood sample to be detected, wherein the detection type at least comprises reagent ineffective detection and aging blood detection;
extracting position information of a wave crest, a starting point and an end point of the wave on the cell volume distribution map;
calculating according to the position information of the wave crest, the starting point and the end point to obtain the peak width ratio of the cell volume distribution map, wherein the peak width ratio is the ratio of the peak value to the width of the wave;
and comparing the detection type, the peak width ratio and the position information of the wave crest with a preset waveform characteristic threshold value to determine whether the blood sample to be detected is abnormal or not.
Further, when the detection type is a reagent invalid detection, comparing the position information of the peak width ratio, the peak and the detection type with a preset waveform characteristic threshold value to determine whether the blood sample to be detected is abnormal, including:
calculating the ratio of the peak width ratio to a preset constant term to obtain the white blood cell number of the blood sample to be detected;
and comparing the peak width ratio, the position information of the wave crest, the white blood cell number and a preset waveform characteristic threshold value, and judging whether the reagent reacting with the blood sample to be detected is invalid.
Further, the step of comparing the peak width ratio, the position information of the peak, the white blood cell number with a preset waveform characteristic threshold value to determine whether the reagent reacting with the blood sample to be detected is invalid, specifically includes:
confirming that the reagent reacting with the blood sample to be tested is not effective when only one peak exists in the cell volume distribution diagram and the following conditions are satisfied:
the cell volume corresponding to the wave crest is smaller than a preset cell volume threshold value;
the white blood cell count of which the peak width ratio is greater than a preset multiple;
the white blood cell count is greater than a preset white blood cell threshold.
Further, the step of comparing the peak width ratio, the position information of the peak, the white blood cell number with a preset waveform characteristic threshold value to determine whether the reagent reacting with the blood sample to be detected is invalid, and the method specifically includes:
when a plurality of peaks exist in the cell volume distribution diagram, acquiring a left slope and a right slope of each peak;
confirming that the reagent reacting with the blood sample to be tested is not effective when the following conditions are simultaneously satisfied:
the absolute value of the left slope or the right slope is larger than a preset slope threshold;
the white blood cell count of which the peak width ratio is greater than a preset multiple;
the white blood cell count is greater than a preset white blood cell count threshold.
Further, when the detection type of the blood sample to be detected is aged blood detection, the calculating according to the position information of the peak, the starting point and the end point to obtain the peak-to-width ratio of the cell volume distribution map includes:
dividing the region from the starting point to the ending point into three regions according to a preset dividing rule, wherein a first region comprises the starting point, a third region comprises the ending point, the first region is adjacent to a second region, and the second region is adjacent to the third region;
determining a third peak width of the third region according to the position information of the third region;
and calculating according to the third peak width and the peak value of the peak in the third region to obtain a third peak width ratio of the third region.
Further, the determining whether the blood sample to be detected is abnormal according to the detection type, the peak width ratio and the position information of the peak and a preset waveform characteristic threshold value includes:
calculating according to the peak value of the peak in the first area and the peak value of the peak in the third area to obtain a peak value ratio;
determining a first peak width of the first region and a second peak width of the second region according to the position information of the first region and the second region;
calculating the product of the peak value of the peak in the first area and the first peak width and the product of the peak value of the peak in the third area and the third peak width to obtain a first target value and a third target value;
and comparing the position information of the wave crest in the third area, the peak value ratio, the second peak width, the third peak width, the first target value and the third target value with a preset waveform characteristic threshold value to determine whether the blood sample to be detected is abnormal.
Further, the determining whether the blood sample to be detected is abnormal according to the position information of the peak in the third area, the peak ratio, the second peak width, the third peak width, the first target value and the third target value compared with a preset waveform characteristic threshold value specifically includes:
and when the following conditions are met, confirming that the blood sample to be detected is aged blood:
the cell volume size corresponding to the wave peak in the third area is smaller than a preset cell volume threshold value;
the peak value ratio is smaller than a preset peak value ratio threshold value;
the second peak width is smaller than a preset peak width threshold value;
the ratio of the first target value to the third target value is smaller than a preset ratio threshold;
the third peak-to-width ratio is less than a preset peak-to-width ratio threshold.
To achieve the above object, a second aspect of the present application provides a blood sample detection device, the device comprising: the device comprises a sample acquisition unit, a parameter extraction unit and a sample detection unit;
the sample acquisition unit is used for acquiring a cell volume distribution diagram of a blood sample to be detected and a detection type of the blood sample to be detected, wherein the detection type at least comprises reagent invalid detection and aging blood detection;
the parameter extraction unit is used for extracting the position information of the wave crest, the starting point and the end point of the upper wave of the cell volume distribution map;
calculating according to the position information of the wave crest, the starting point and the end point to obtain the peak width ratio of the cell volume distribution map, wherein the peak width ratio is the ratio of the peak value to the width of the wave;
the sample detection unit is used for comparing the detection type, the peak width ratio and the position information of the wave crest with a preset waveform characteristic threshold value to determine whether the blood sample to be detected is abnormal or not.
To achieve the above object, a third aspect of the present application provides a computer readable storage medium storing a computer program, wherein the computer program, when executed by a processor, causes the processor to perform the steps of the method according to the first aspect.
To achieve the above object, a fourth aspect of the present application provides a computer device comprising a memory and a processor, characterized in that the memory stores a computer program, which, when executed by the processor, causes the processor to perform the steps of the method according to the first aspect.
The embodiment of the invention has the following beneficial effects:
the embodiment of the invention provides a method for detecting a blood sample, which comprises the following steps: obtaining a cell volume distribution diagram of a blood sample to be detected and a detection type of the blood sample to be detected, wherein the detection type at least comprises reagent invalid detection and aging blood detection; extracting position information of a wave crest, a starting point and an end point of the wave on the cell volume distribution map; calculating according to the position information of the wave crest, the starting point and the end point to obtain the peak width ratio of the cell volume distribution map, wherein the peak width ratio is the ratio of the peak value to the width of the wave; and comparing the detection type, the peak width ratio and the position information of the wave crest with a preset waveform characteristic threshold value to determine whether the blood sample to be detected is abnormal. According to the scheme, through obtaining a cell volume distribution diagram of the distribution condition of the cell volume of the blood sample to be detected, parameters for judgment, such as a peak, a starting point, an end point and the like, are obtained according to the cell volume distribution diagram, the parameters are compared with a preset waveform characteristic threshold according to the type of the problem to be detected, whether the blood sample to be detected is abnormal or not is judged, and the problem that serious loss is caused to hospitals and patients due to the fact that the abnormal blood sample is found in time is solved.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Wherein:
FIG. 1 is a flow chart of a method for detecting a blood sample according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of the operation of the detecting instrument;
FIG. 3 is a graph of white blood cell count WBC versus peak width ratio PWR for an embodiment of the present invention;
FIG. 4 is a graph of normal cell volume distribution for the practice of the present invention;
FIG. 5 is a graph showing a cell volume distribution of aged blood according to an embodiment of the present invention;
FIG. 6 is a block diagram showing a blood sample testing device according to an embodiment of the present invention;
fig. 7 is an internal structural diagram of a computer device in the embodiment of the present application.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Blood analysis typically uses a reagent to interact with a blood sample to alter the morphology of different leukocytes in the blood and to analyze the morphology of statistically different leukocytes.
However, the detection result may be affected by the deterioration of the reagent, the shortage of the reagent, or the aging of the blood sample, thereby causing various losses.
For example, when the reagent is deteriorated or a sufficient amount of reagent is not added, the classification result of the white blood cells is distorted; or the test of the blood sample has timeliness, and after the blood leaves the human body for a period of time or other influences, some components in the blood sample deteriorate to form aged blood, so that the detection result is distorted. The result of the test is distorted causing false positives to be lost to hospitals and patients.
Based on this, an embodiment of the present invention provides a method for detecting a blood sample, referring to fig. 1, fig. 1 is a flow chart of the method for detecting a blood sample according to an embodiment of the present invention, so as to reduce probability of distortion of a detection result, and specifically includes:
step 110, obtaining a cell volume distribution diagram of a blood sample to be detected and a detection type of the blood sample to be detected, wherein the detection type at least comprises reagent ineffective detection and aging blood detection.
Specifically, the white blood cell volume distribution of a blood sample can be obtained by a three-class hemocytometer, the horizontal axis of the cell volume distribution map represents the volume size of cells, and the vertical axis represents the white blood cell number.
The three-classification hemocytometer is a blood analyzer widely used in basic medical institutions. The method has the main advantages of convenience, rapidness and low cost, and the approximate flow of the cell test of the three-class blood analyzer is that the blood sample to be detected enters the instrument and is diluted by the diluent and is treated by the reagent, and the different types of white blood cells react with the reagent to different degrees and are divided into three types of white blood cells with different sizes. All white blood cells pass through the small hole of the precious stone, and referring to fig. 2, fig. 2 is a working principle diagram of a detection instrument, an analysis circuit is added to the detection instrument, constant current sources are added to two ends of the small hole, and then the processed three types of white blood cells are added to the detection instrument for particle detection. Each leukocyte will generate a voltage pulse signal with peak height, which is the volume of the leukocyte. Therefore, based on the principle that the peak height of the voltage pulse signal is in direct proportion to the volume of the white blood cells when the white blood cells pass through the small holes, the number distribution condition of the volume of the white blood cells is counted, and a cell volume distribution diagram is obtained.
Step 120, extracting position information of the peak, the start point and the end point of the wave on the cell volume distribution map.
Specifically, positional information of the peak, the start point, and the end point of the wave is acquired based on the cell volume distribution map, and the positional information may be coordinate information. For example, the position information of the peak is P N (P NX ，P NY ) (N is an integer greater than 0) the origin of the wave is Z L (Z l 0), the end point of the wave is Z R (Z r ,0). Wherein the starting point Z L Z L (Z l 0) may be the first peak P from left in the off-cell volume distribution map 1 The nearest point with zero ordinate; similarly, endpoint Z R (Z r 0) may be the last peak P from left in the off-cell volume distribution map N The nearest point with zero ordinate.
Step130, calculating according to the position information of the peak, the starting point and the end point to obtain the peak width ratio of the cell volume distribution map, wherein the peak width ratio is the ratio of the peak value to the width of the wave.
Specifically, the peak width ratio may be pwr=mean (P 1Y 、P 2Y 、P 3Y 、……、P NY )/(Z r -Z l ) Where mean () is the average value.
And 140, comparing the position information of the peak width ratio and the peak with a preset waveform characteristic threshold value according to the detection type, the peak width ratio and the position information of the peak, and determining whether the blood sample to be detected is abnormal or not.
In order to better determine whether a blood sample to be detected has a certain problem, the embodiment of the invention proposes to select different comparison methods according to different conditions to determine whether the blood sample to be detected has an abnormality.
According to the embodiment of the invention, the cell volume distribution diagram of the cell volume distribution situation of the blood sample to be detected is obtained, the wave crest, the starting point and the end point are obtained according to the cell volume distribution diagram, the parameters are compared with the preset waveform characteristic threshold according to the type of the problem to be detected, whether the blood sample to be detected is abnormal or not is judged, and the problem that the serious loss is caused to hospitals and patients due to the fact that the final blood analysis result is distorted is avoided by timely finding out the abnormal blood sample.
The following describes a specific implementation method for detecting whether the sample to be detected is abnormal under different abnormal types:
if it is desired to determine whether the blood sample to be detected has an abnormal condition that the reagent is invalid, step140, when the detection type is the reagent invalid detection, comparing the position information of the detection type, the peak width ratio and the peak with a preset waveform characteristic threshold value, and determining whether the blood sample to be detected is abnormal, specifically includes:
step1401, calculating the ratio of the peak width ratio to a preset constant term to obtain the white blood cell number of the blood sample to be detected.
Since the peak width ratio PWR is a parameter strongly related to the white blood cell count WBC, the relationship is shown in fig. 3, fig. 3 is a graph showing the relationship between the white blood cell count WBC and the peak width ratio PWR according to the embodiment of the present invention, and the approximate relationship is pwr=k 1 XWBC, where K 1 Is a predetermined first constant term, which is an empirical value. Thus, after the peak width ratio PWR is obtained, the peak width ratio PWR and the first constant term K can be used 1 The ratio of (2) to (c) to obtain the white blood cell number WBC.
Step1402, comparing the peak width ratio, the position information of the peak, the white blood cell number and the preset waveform characteristic threshold value, and judging whether the reagent reacting with the blood sample to be detected is invalid.
Specifically, the abnormal condition of reagent inefficiency may include that the reagent is abnormal or the amount of the reagent placed is insufficient, and when the reagent is ineffective, the effect of the reagent on the white blood cells is ineffective or greatly weakened, and the morphological characteristics of the white blood cell histogram are obviously different from those of the sample when the reagent is completely effective.
When the reagent is effective, the obtained cell volume distribution diagram in the ideal state should have two obvious peaks, but one or more peaks may appear in the actual situation, so as to obtain a more accurate detection result and reduce the probability of misjudgment, the embodiment of the invention analyzes the two situations respectively.
(1) If there is only one peak in the cell volume distribution map, step1402, based on the peak width ratio, the position information of the peak, the white blood cell count and the preset waveform characteristic threshold, determines whether the reagent reacting with the blood sample to be detected is ineffective, which may specifically include: when only one peak exists in the cell volume distribution diagram, and the following conditions are met, the reagent reacting with the blood sample to be detected is confirmed to be invalid:
the cell volume corresponding to the wave crest is smaller than a preset cell volume threshold value;
white blood cell count with peak width ratio greater than preset multiple;
the number of leukocytes is greater than a preset leukocyte threshold.
Specifically, the preset cell volume threshold may be 50; the preset multiple of the number of the white blood cells can be K 2 ×K 1 XWBC, where K 2 Is a preset second constant term, K 1 Is a preset first constant term; the predetermined cell threshold may be 1.0X10-9/L.
(2) If there are multiple peaks in the cell volume distribution map, step1402, comparing the peak width ratio, the position information of the peaks, the white blood cell count with a preset waveform characteristic threshold, and judging whether the reagent reacting with the blood sample to be detected is invalid, may specifically further include: when a plurality of peaks exist in the cell volume distribution diagram, acquiring a left slope and a right slope of each peak; at the same time, the reagent reacting with the blood sample to be detected is confirmed to be invalid when the following conditions are satisfied:
the absolute value of the left slope or the right slope is larger than a preset slope threshold value;
white blood cell count with peak width ratio greater than preset multiple;
the white blood cell count is greater than a preset white blood cell count threshold.
Specifically, the left slope and the right slope are slopes of a peak point and two points adjacent to each other left and right of the cell volume distribution map, respectively. For example, the peak point P N The left and right slopes of (a) can be expressed as K NL And K NR The method comprises the steps of carrying out a first treatment on the surface of the The preset slope threshold may be 3, i.e., the left slopes of all peaks are greater than 3, or the absolute values of the right slopes of all peaks are greater than 3; the preset multiple of the number of the white blood cells can be K 3 ×K 1 XWBC, where K 3 Is a preset third constant term, K 1 Is a preset first constant term; the predetermined cell threshold may be 1.0X10-9/L.
If it is desired to determine whether the blood sample to be detected has an abnormal condition of aging blood, then step130, calculating according to the position information of the peak, the start point and the end point to obtain the peak-to-width ratio of the cell volume distribution diagram, further includes:
step1301, dividing a region from a start point to an end point into three regions according to a preset dividing rule, wherein a first region comprises the start point, a third region comprises the end point, the first region is adjacent to a second region, and the second region is adjacent to the third region.
Step1302, determining a third peak width of the third region according to the position information of the third region.
Step1303, calculating according to the third peak width and the peak value of the peak in the third area, to obtain the third peak width ratio of the third area.
Currently, no alarm for aging blood is provided, and the current judgment for aging blood is only based on the clinical experience of doctors. Aging is accelerated due to the specificity of the blood sample or the different sample storage environments. Thus, the aged blood is mistakenly used as fresh blood for detection, and the false positive is caused by the distortion of the result of the aged blood test, so that the loss is caused to hospitals and patients.
In general, the white blood cell volume distribution map is divided into three parts when the reagent is fully effective, as shown in fig. 4, fig. 4 is a normal cell volume distribution map of the present invention, and three areas of the white blood cell volume distribution map divided by three dotted lines correspond to three types of white blood cells. The first region and the third region have a distinct peak, respectively. When the blood is degenerated and aged, the cell activity of the white blood cells is changed, the effect of the reagent on the white blood cells is weakened, and the white blood cell volume distribution diagram can be referred to as fig. 5, and fig. 5 shows the cell volume distribution diagram of the aged blood according to the embodiment of the invention, wherein the morphological characteristics of the aged blood are obviously different from those of a normal blood sample. Therefore, the embodiment of the invention judges whether the sample to be detected is aged blood or not through three classification and analysis on the cell volume distribution diagram.
After the third peak width ratio obtained in the above manner, step140 compares the position information of the peak width ratio, the peak and the detection type with a preset waveform characteristic threshold value to determine whether the blood sample to be detected is abnormal, which specifically includes:
step1403, calculating according to the peak value of the peak in the first area and the peak value of the peak in the third area, and obtaining the peak value ratio.
Step1403, determining a first peak width of the first region and a second peak width of the second region according to the position information of the first region and the second region.
Specifically, the first peak width is the distance between two lines dividing the first region, and the second peak width is the distance between two lines dividing the second region.
Step1403, calculating the product of the peak value of the peak in the first region and the first peak width, and the product of the peak value of the peak in the third region and the third peak width, to obtain a first target value and a third target value.
Step1403, comparing the position information of the wave crest in the third area, the peak value ratio, the second peak width, the third peak width, the first target value and the third target value with a preset waveform characteristic threshold value, and determining whether the blood sample to be detected is abnormal.
Optionally, the determining method specifically includes, when the following conditions are satisfied at the same time, confirming that the blood sample to be detected is aged blood:
the cell volume size corresponding to the wave peak in the third area is smaller than a preset cell volume threshold value;
the peak value ratio is smaller than a preset peak value ratio threshold value;
the second peak width is smaller than a preset peak width threshold value;
the ratio of the first target value to the third target value is smaller than a preset ratio threshold;
the third peak-to-width ratio is less than a preset peak-to-width ratio threshold.
Specifically, the preset cell volume threshold may be 50; the preset peak ratio threshold may be 1.1; the preset peak width threshold may be 0.2× (W 1 ×W 1 /(W 1 +W 2 )+W 2 ×W 2 /(W 1 +W 2 ) And, wherein W 1 For a first peak width, W 2 Is the second peak width; the preset ratio threshold may be 0.3; the preset peak-to-width ratio threshold may be 1.3.
According to the embodiment of the invention, different detection methods are provided for different detection types, the accuracy of detection results is improved, and the probability of occurrence of distortion of the detection results is effectively reduced.
The embodiment of the invention further provides a device for detecting a blood sample, referring to fig. 6, fig. 6 is a block diagram of a structure of the device for detecting a blood sample according to the embodiment of the invention, where the device includes: a sample acquisition unit 601, a parameter extraction unit 602, and a sample detection unit 603.
A sample acquiring unit 601, configured to acquire a cell volume distribution map of a blood sample to be detected, and a detection type of the blood sample to be detected, where the detection type includes at least a reagent invalid detection and an aging blood detection;
a parameter extraction unit 602 for extracting position information of a peak, a start point, and an end point of the wave on the cell volume distribution map; and calculating according to the position information of the wave crest, the starting point and the end point to obtain the peak width ratio of the cell volume distribution map, wherein the peak width ratio is the ratio of the peak value to the width of the wave.
The sample detection unit 603 is configured to determine whether the blood sample to be detected is abnormal according to the detection type, the peak width ratio, and the position information of the peak and a preset waveform characteristic threshold.
According to the detection device for the blood sample, provided by the embodiment of the invention, through obtaining the cell volume distribution diagram of the distribution condition of the cell volume of the blood sample to be detected, parameters for judgment, such as a peak, a starting point, a terminal point and the like, are obtained according to the cell volume distribution diagram, the parameters are compared with the preset waveform characteristic threshold according to the type of the problem to be detected, whether the blood sample to be detected is abnormal or not is judged, and the problem that serious loss is caused to hospitals and patients due to the fact that the abnormal blood sample is found in time is solved.
FIG. 7 shows an internal block diagram of a computer device in one embodiment of the invention. The computer device may specifically be a terminal or a system. As shown in fig. 7, the computer device includes a processor, a memory, and a network interface connected by a system bus. The memory includes a nonvolatile storage medium and an internal memory. The non-volatile storage medium of the computer device stores an operating system, and may also store a computer program which, when executed by a processor, causes the processor to implement the steps of the method embodiments described above. The internal memory may also have stored therein a computer program which, when executed by a processor, causes the processor to perform the steps of the method embodiments described above. It will be appreciated by those skilled in the art that the structure shown in fig. 7 is merely a block diagram of some of the structures associated with the present application and is not limiting of the computer device to which the present application may be applied, and that a particular computer device may include more or fewer components than shown, or may combine certain components, or have a different arrangement of components.
In one embodiment, a computer device is provided that includes a memory and a processor, the memory storing a computer program that, when executed by the processor, causes the processor to perform the steps of the method embodiments described above.
In one embodiment, a computer-readable storage medium is provided, in which a computer program is stored which, when executed by a processor, causes the processor to perform the steps of the method embodiments described above.
Those skilled in the art will appreciate that the processes implementing all or part of the methods of the above embodiments may be implemented by a computer program for instructing relevant hardware, and the program may be stored in a non-volatile computer readable storage medium, and the program may include the processes of the embodiments of the methods as above when executed. Any reference to memory, storage, database, or other medium used in the various embodiments provided herein may include non-volatile and/or volatile memory. The nonvolatile memory can include Read Only Memory (ROM), programmable ROM (PROM), electrically Programmable ROM (EPROM), electrically Erasable Programmable ROM (EEPROM), or flash memory. Volatile memory can include Random Access Memory (RAM) or external cache memory. By way of illustration and not limitation, RAM is available in a variety of forms such as Static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), double Data Rate SDRAM (DDRSDRAM), enhanced SDRAM (ESDRAM), synchronous Link DRAM (SLDRAM), memory bus direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM), among others.
The technical features of the above embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The foregoing examples represent only a few embodiments of the present application, which are described in more detail and are not thereby to be construed as limiting the scope of the present application. It should be noted that it would be apparent to those skilled in the art that various modifications and improvements could be made without departing from the spirit of the present application, which would be within the scope of the present application. Accordingly, the scope of protection of the present application is to be determined by the claims appended hereto.
Claims (10)
1. A method of testing a blood sample, the method comprising:
obtaining a cell volume distribution diagram of a blood sample to be detected and a detection type of the blood sample to be detected, wherein the detection type at least comprises reagent ineffective detection and aging blood detection;
extracting position information of a wave crest, a starting point and an end point of the wave on the cell volume distribution map;
calculating according to the position information of the wave crest, the starting point and the end point to obtain the peak width ratio of the cell volume distribution map, wherein the peak width ratio is the ratio of the peak value to the width of the wave;
and comparing the detection type, the peak width ratio and the position information of the wave crest with a preset waveform characteristic threshold value to determine whether the blood sample to be detected is abnormal or not.
2. The method according to claim 1, wherein when the detection type is a reagent-ineffective detection, the determining whether the blood sample to be detected is abnormal according to the detection type, the peak-to-width ratio, and the position information of the peak compared with a preset waveform feature threshold value, specifically includes:
calculating the ratio of the peak width ratio to a preset constant term to obtain the white blood cell number of the blood sample to be detected;
and comparing the peak width ratio, the position information of the wave crest, the white blood cell number and a preset waveform characteristic threshold value, and judging whether the reagent reacting with the blood sample to be detected is invalid.
3. The method according to claim 2, wherein the determining whether the reagent reacting with the blood sample to be detected is invalid according to the peak width ratio, the position information of the peak, the white blood cell count and a preset waveform characteristic threshold value comprises:
confirming that the reagent reacting with the blood sample to be tested is not effective when only one peak exists in the cell volume distribution diagram and the following conditions are satisfied:
the cell volume corresponding to the wave crest is smaller than a preset cell volume threshold value;
the white blood cell count of which the peak width ratio is greater than a preset multiple;
the white blood cell count is greater than a preset white blood cell threshold.
4. The method according to claim 2, wherein the determining whether the reagent reacting with the blood sample to be detected is invalid according to the peak width ratio, the position information of the peak, the white blood cell count and a preset waveform characteristic threshold value comprises:
when a plurality of peaks exist in the cell volume distribution diagram, acquiring a left slope and a right slope of each peak;
confirming that the reagent reacting with the blood sample to be tested is not effective when the following conditions are simultaneously satisfied:
the absolute value of the left slope or the right slope is larger than a preset slope threshold;
the white blood cell count of which the peak width ratio is greater than a preset multiple;
the white blood cell count is greater than a preset white blood cell count threshold.
5. The method according to claim 1, wherein when the detection type of the blood sample to be detected is aged blood detection, the calculating according to the position information of the peak, the start point and the end point to obtain the peak-to-width ratio of the cell volume distribution map includes:
dividing the region from the starting point to the ending point into three regions according to a preset dividing rule, wherein a first region comprises the starting point, a third region comprises the ending point, the first region is adjacent to a second region, and the second region is adjacent to the third region;
determining a third peak width of the third region according to the position information of the third region;
and calculating according to the third peak width and the peak value of the peak in the third region to obtain a third peak width ratio of the third region.
6. The method according to claim 5, wherein the determining whether the blood sample to be detected is abnormal according to the detection type, the peak width ratio and the position information of the peak is compared with a preset waveform characteristic threshold value, specifically comprises:
calculating according to the peak value of the peak in the first area and the peak value of the peak in the third area to obtain a peak value ratio;
determining a first peak width of the first region and a second peak width of the second region according to the position information of the first region and the second region;
calculating the product of the peak value of the peak in the first area and the first peak width and the product of the peak value of the peak in the third area and the third peak width to obtain a first target value and a third target value;
and comparing the position information of the wave crest in the third area, the peak value ratio, the second peak width, the third peak width, the first target value and the third target value with a preset waveform characteristic threshold value to determine whether the blood sample to be detected is abnormal.
7. The method according to claim 6, wherein the determining whether the blood sample to be detected is abnormal according to the position information of the peak in the third area, the peak ratio, the second peak width, the third peak width, the first target value and the third target value compared with a preset waveform characteristic threshold value specifically comprises:
and when the following conditions are met, confirming that the blood sample to be detected is aged blood:
the cell volume size corresponding to the wave peak in the third area is smaller than a preset cell volume threshold value;
the peak value ratio is smaller than a preset peak value ratio threshold value;
the second peak width is smaller than a preset peak width threshold value;
the ratio of the first target value to the third target value is smaller than a preset ratio threshold;
the third peak-to-width ratio is less than a preset peak-to-width ratio threshold.
8. A device for testing a blood sample, the device comprising: the device comprises a sample acquisition unit, a parameter extraction unit and a sample detection unit;
the sample acquisition unit is used for acquiring a cell volume distribution diagram of a blood sample to be detected and a detection type of the blood sample to be detected, wherein the detection type at least comprises reagent invalid detection and aging blood detection;
the parameter extraction unit is used for extracting the position information of the wave crest, the starting point and the end point of the upper wave of the cell volume distribution map;
calculating according to the position information of the wave crest, the starting point and the end point to obtain the peak width ratio of the cell volume distribution map, wherein the peak width ratio is the ratio of the peak value to the width of the wave;
the sample detection unit is used for comparing the detection type, the peak width ratio and the position information of the wave crest with a preset waveform characteristic threshold value to determine whether the blood sample to be detected is abnormal or not.
9. A computer readable storage medium storing a computer program, which when executed by a processor causes the processor to perform the steps of the method according to any one of claims 1 to 7.
10. A computer device comprising a memory and a processor, wherein the memory stores a computer program which, when executed by the processor, causes the processor to perform the steps of the method of any of claims 1 to 7.
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| CN202311802833.5A CN117825242A (en) | 2023-12-25 | 2023-12-25 | Method and device for detecting blood sample, equipment and storage medium |
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| CN202311802833.5A CN117825242A (en) | 2023-12-25 | 2023-12-25 | Method and device for detecting blood sample, equipment and storage medium |
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