CN117783385A - Method for detecting fat inhibition effect - Google Patents
Method for detecting fat inhibition effect Download PDFInfo
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- CN117783385A CN117783385A CN202311822268.9A CN202311822268A CN117783385A CN 117783385 A CN117783385 A CN 117783385A CN 202311822268 A CN202311822268 A CN 202311822268A CN 117783385 A CN117783385 A CN 117783385A
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- fat
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- detecting
- suppression effect
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- 230000000694 effects Effects 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 45
- 230000005764 inhibitory process Effects 0.000 title claims abstract description 27
- 238000000605 extraction Methods 0.000 claims abstract description 44
- 238000000227 grinding Methods 0.000 claims abstract description 22
- 238000001035 drying Methods 0.000 claims abstract description 19
- 239000003925 fat Substances 0.000 claims description 121
- 239000003463 adsorbent Substances 0.000 claims description 38
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 23
- 230000001629 suppression Effects 0.000 claims description 22
- 238000004458 analytical method Methods 0.000 claims description 14
- 239000003960 organic solvent Substances 0.000 claims description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- 238000005303 weighing Methods 0.000 claims description 10
- 239000012535 impurity Substances 0.000 claims description 8
- 239000000049 pigment Substances 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 6
- 238000000944 Soxhlet extraction Methods 0.000 claims description 5
- 238000004817 gas chromatography Methods 0.000 claims description 5
- 239000003208 petroleum Substances 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 238000002137 ultrasound extraction Methods 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 23
- 238000000746 purification Methods 0.000 abstract description 8
- 230000002452 interceptive effect Effects 0.000 abstract description 4
- 238000004806 packaging method and process Methods 0.000 abstract 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- 230000006978 adaptation Effects 0.000 description 2
- 238000004042 decolorization Methods 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 125000005457 triglyceride group Chemical group 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
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- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention provides a method for detecting fat inhibition effect, which belongs to the technical field of fat detection and comprises the following steps: firstly, obtaining a sample to be detected, placing the sample into a grinding device, controlling the speed of the grinding device to grind the sample for a period of time, taking out the ground sample, and collecting and packaging the sample in multiple groups, wherein fat is extracted, and a proper extraction method is selected when the fat is extracted; according to the method, fat extraction is carried out by arranging a plurality of different methods, the best extraction method can be selected for fat extraction, so that the effect on fat extraction can be effectively improved, meanwhile, purification treatment operations such as decoloring, drying and the like specially aiming at fat are arranged at the follow-up, and by purifying the fat, some uncertain factors interfering with detection can be removed, so that the detection accuracy and detection effect on fat inhibition effect can be greatly improved, and the practical application effect of the method is greatly improved.
Description
Technical Field
The invention relates to the technical field of fat detection, in particular to a method for detecting fat inhibition effect.
Background
The oily substances in foods are mainly oils and fats, and are generally referred to as oils which are liquid at ordinary temperatures and fats which are solid at ordinary temperatures. Fat consists of C, H, O elements. Fat is triglyceride composed of glycerin and fatty acid, wherein the glycerin has relatively simple molecules, the types and the lengths of the fatty acid are different, a plurality of fats are also in animal bodies, the fat is a protective umbrella with good internal organs, but too much fat can have adverse effects, and in order to detect the inhibition effect of the fat after inhibition, a fat inhibition effect detection method needs to be applied.
In the existing fat suppression effect detection methods, although detection results can be obtained, when fat extraction is performed, a proper treatment method is not adopted, so that the fat extraction effect is poor, meanwhile, purification treatment operations such as color removal and drying are not performed on the fat, so that the accuracy of the final detection result cannot be ensured, the actual application effect of the whole method is poor, and in order to solve the problems, a detection method for the fat suppression effect is needed.
Summary of the invention
In view of the above, the present invention provides a method for detecting fat suppression effect, which can accurately and efficiently detect fat suppression effect.
In order to solve the technical problems that the detection result can be obtained by the existing fat suppression effect detection method, but when fat extraction is carried out, a proper treatment method is not adopted, so that the fat extraction effect is poor, and meanwhile, the purification treatment operations such as color removal and drying are not carried out on the fat, so that the final detection result precision cannot be ensured, and the actual application effect of the whole method is poor, the application of the invention provides a detection method for the fat suppression effect, which comprises the following steps:
s1, firstly, acquiring a sample to be detected;
s2, placing the sample in a grinding device, and controlling the speed of the grinding device to grind the sample for a period of time;
s3, taking out the ground sample, and dividing the sample into multiple groups for collection;
s4, extracting fat;
s5, selecting a proper extraction method when fat is extracted;
s6, placing the fat into a decolorizer, adding a proper amount of adsorbent, and fully stirring to enable the adsorbent to fully contact the fat and adsorb pigments and impurities in the fat;
s7, standing for a period of time, filtering the adsorbent after the adsorbent is fully adsorbed, and drying the fat;
s8, repeating the steps for a plurality of times until the color of the fat meets the requirement, and drying the fat;
s9, quantitatively analyzing the purified fat by adopting an analysis method;
s10, detecting fat inhibition effect.
As a further description of the above technical solution:
in the step S1, a sample to be detected is firstly obtained, the sample is weighed, and weighing data are recorded.
As a further description of the above technical solution:
in the step S2, the sample is placed in a grinding device, the speed of the grinding device is controlled to be 60-80r/min, and the sample is ground for 25-30min.
As a further description of the above technical solution:
and in the step S3, taking out the ground sample, and collecting the sample in 3-5 groups.
As a further description of the above technical solution:
in the step S4, fat is extracted, and the specific steps are as follows: firstly placing a sample in an extraction container, adding an organic solvent into the container, and extracting fat in the container, wherein the organic solvent is one or more of diethyl ether and petroleum ether.
As a further description of the above technical solution:
in the step S5, a suitable extraction method is selected when fat extraction is performed, and the extraction method is one of a soxhlet extraction method and an ultrasonic assisted extraction method.
As a further description of the above technical solution:
in the step S6, the fat is placed in a decolorizer, a proper amount of adsorbent is added, and the mixture is fully stirred to enable the adsorbent to fully contact the fat, so that pigment and impurities in the fat are adsorbed, and the adsorbent is one or more of activated carbon and silica gel.
As a further description of the above technical solution:
and in the step S7, standing for 20-25min, filtering the adsorbent after the adsorbent is fully adsorbed, and drying the fat.
As a further description of the above technical solution:
in S9, the purified fat is quantitatively analyzed by an analysis method, and the analysis method is one of gas chromatography and high performance liquid chromatography.
As a further description of the above technical solution:
in S10, the fat-suppression effect is detected by comparing the fat content changes before and after the sample treatment. If the fat content of the sample after treatment is reduced, the fat inhibition effect is better; if the fat content after the sample treatment is increased, it is indicated that the fat-suppression effect is poor.
In summary, compared with the prior art, the present application includes at least one of the following beneficial technical effects:
according to the method, fat extraction is carried out by arranging a plurality of different methods, the best extraction method can be selected for fat extraction, so that the effect on fat extraction can be effectively improved, meanwhile, purification treatment operations such as decoloring, drying and the like specially aiming at fat are arranged at the follow-up, and by purifying the fat, some uncertain factors interfering with detection can be removed, so that the detection accuracy and detection effect on fat inhibition effect can be greatly improved, and the practical application effect of the method is greatly improved.
Drawings
Fig. 1 is a flowchart of a method for detecting fat suppression effect according to the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present application more clear, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to fig. 1 of the embodiments of the present application. It will be apparent that the described embodiments are some, but not all, of the embodiments of the present application. All other embodiments, which are obtained by a person skilled in the art based on the described embodiments of the present application, are within the scope of the present application.
Example 1
Referring to fig. 1, the present invention provides a technical solution: a method for detecting fat suppression effect comprises the following steps:
s1, firstly acquiring a sample to be detected, weighing the sample, and recording weighing data;
s2, placing the sample in a grinding device, controlling the speed of the grinding device to 60r/min, and grinding the sample for 25min;
s3, taking out the ground samples, and collecting and assembling the samples in 5 groups;
s4, extracting fat, wherein the specific steps are as follows: firstly, placing a sample in an extraction container, adding an organic solvent into the container, and extracting fat in the container, wherein the organic solvent is petroleum ether;
s5, selecting a proper extraction method when fat is extracted, wherein the extraction method is a Soxhlet extraction method;
s6, placing the fat into a decolorizer, adding a proper amount of adsorbent, fully stirring the fat, fully contacting the adsorbent with the fat, and adsorbing pigment and impurities in the fat, wherein the adsorbent is activated carbon;
s7, standing for 20min, filtering the adsorbent after the adsorbent is fully adsorbed, and drying the fat;
s8, repeating the steps for a plurality of times until the color of the fat meets the requirement, and drying the fat;
s9, quantitatively analyzing the purified fat by adopting an analysis method, wherein the analysis method is a gas chromatography;
s10, detecting fat inhibition effect, namely comparing fat content changes before and after sample treatment to detect the fat inhibition effect. If the fat content of the sample after treatment is reduced, the fat inhibition effect is better; if the fat content after the sample treatment is increased, it is indicated that the fat-suppression effect is poor.
In the embodiment, the method can remove some uncertain factors interfering with detection through the purification treatment of fat, so that the detection accuracy and detection effect on fat inhibition effect can be greatly improved, and the practical application effect of the method is greatly improved.
Example 2
Referring to fig. 1, the present invention provides a technical solution: a method for detecting fat suppression effect comprises the following steps:
s1, firstly acquiring a sample to be detected, weighing the sample, and recording weighing data;
s2, placing the sample in a grinding device, controlling the speed of the grinding device to 70r/min, and grinding the sample for 28min;
s3, taking out the ground samples, and collecting and assembling the samples in groups 4;
s4, extracting fat, wherein the specific steps are as follows: firstly, placing a sample in an extraction container, adding an organic solvent into the container, and extracting fat in the container, wherein the organic solvent is diethyl ether and petroleum ether;
s5, selecting a proper extraction method when fat is extracted, wherein the extraction method is an ultrasonic auxiliary extraction method;
s6, placing the fat into a decolorizer, adding a proper amount of adsorbent, and fully stirring to enable the adsorbent to fully contact the fat, so as to adsorb pigment and impurities in the fat, wherein the adsorbent is silica gel;
s7, standing for 23min, filtering the adsorbent after the adsorbent is fully adsorbed, and drying the fat;
s8, repeating the steps for a plurality of times until the color of the fat meets the requirement, and drying the fat;
s9, quantitatively analyzing the purified fat by adopting an analysis method, wherein the adopted analysis method is high performance liquid chromatography;
s10, detecting fat inhibition effect, namely comparing fat content changes before and after sample treatment to detect the fat inhibition effect. If the fat content of the sample after treatment is reduced, the fat inhibition effect is better; if the fat content after the sample treatment is increased, it is indicated that the fat-suppression effect is poor.
In this embodiment, the method is provided with a plurality of different methods for fat extraction, and the best extraction method can be selected for fat extraction, so that the effect on fat extraction can be effectively improved, and purification treatment operations such as decoloring, drying and the like which are specially performed on fat are arranged at the subsequent stage.
Example 3
Referring to fig. 1, the present invention provides a technical solution: a method for detecting fat suppression effect comprises the following steps:
s1, firstly acquiring a sample to be detected, weighing the sample, and recording weighing data;
s2, placing the sample in a grinding device, controlling the speed of the grinding device to 80r/min, and grinding the sample for 30min;
s3, taking out the ground samples, and collecting and assembling the samples in 3 groups;
s4, extracting fat, wherein the specific steps are as follows: firstly, placing a sample in an extraction container, adding an organic solvent into the container, and extracting fat in the container, wherein the organic solvent is diethyl ether;
s5, selecting a proper extraction method when fat is extracted, wherein the extraction method is a Soxhlet extraction method;
s6, placing the fat into a decolorizer, adding a proper amount of adsorbent, fully stirring the fat, fully contacting the adsorbent with the fat, and adsorbing pigment and impurities in the fat, wherein the adsorbent is activated carbon;
s7, standing for 25min, filtering the adsorbent after the adsorbent is fully adsorbed, and drying the fat;
s8, repeating the steps for a plurality of times until the color of the fat meets the requirement, and drying the fat;
s9, quantitatively analyzing the purified fat by adopting an analysis method, wherein the analysis method is a gas chromatography;
s10, detecting fat inhibition effect, namely comparing fat content changes before and after sample treatment to detect the fat inhibition effect. If the fat content of the sample after treatment is reduced, the fat inhibition effect is better; if the fat content after the sample treatment is increased, it is indicated that the fat-suppression effect is poor.
In this embodiment, the method is provided with a plurality of different methods for fat extraction, and the best extraction method can be selected for fat extraction, so that the effect on fat extraction can be effectively improved, and meanwhile, purification treatment operations such as decoloring and drying which are specially aimed at fat are provided at the follow-up, and by means of the purification treatment of the fat, some uncertain factors interfering with detection can be removed, so that the detection accuracy and detection effect on fat inhibition effect can be greatly improved, and the practical application effect of the method is greatly improved. A step of
Example 4
Referring to fig. 1, the present invention provides a technical solution: a method for detecting fat suppression effect comprises the following steps:
s1, firstly acquiring a sample to be detected, weighing the sample, and recording weighing data;
s2, placing the sample in a grinding device, controlling the speed of the grinding device to 75r/min, and grinding the sample for 28min;
s3, taking out the ground samples, and collecting and assembling the samples in 3 groups;
s4, extracting fat, wherein the specific steps are as follows: firstly, placing a sample in an extraction container, adding an organic solvent into the container, and extracting fat in the container, wherein the organic solvent is diethyl ether;
s5, selecting a proper extraction method when fat is extracted, wherein the extraction method is a Soxhlet extraction method;
s6, placing the fat into a decolorizer, adding a proper amount of adsorbent, fully stirring the fat, fully contacting the adsorbent with the fat, and adsorbing pigment and impurities in the fat, wherein the adsorbent is activated carbon;
s7, standing for 22min, filtering the adsorbent after the adsorbent is fully adsorbed, and drying the fat;
s8, repeating the steps for a plurality of times until the color of the fat meets the requirement, and drying the fat;
s9, quantitatively analyzing the purified fat by adopting an analysis method, wherein the analysis method is a gas chromatography;
s10, detecting fat inhibition effect, namely comparing fat content changes before and after sample treatment to detect the fat inhibition effect. If the fat content of the sample after treatment is reduced, the fat inhibition effect is better; if the fat content after the sample treatment is increased, it is indicated that the fat-suppression effect is poor.
While the foregoing is directed to the preferred embodiments of the present invention, it will be appreciated by those skilled in the art that various modifications and adaptations can be made without departing from the principles of the present invention, and such modifications and adaptations are intended to be comprehended within the scope of the present invention.
Claims (10)
1. A method for detecting fat suppression effect is characterized in that: the method comprises the following steps:
s1, firstly, acquiring a sample to be detected;
s2, placing the sample in a grinding device, and controlling the speed of the grinding device to grind the sample for a period of time;
s3, taking out the ground sample, and dividing the sample into multiple groups for collection;
s4, extracting fat;
s5, selecting a proper extraction method when fat is extracted;
s6, placing the fat into a decolorizer, adding a proper amount of adsorbent, and fully stirring to enable the adsorbent to fully contact the fat and adsorb pigments and impurities in the fat;
s7, standing for a period of time, filtering the adsorbent after the adsorbent is fully adsorbed, and drying the fat;
s8, repeating the steps for a plurality of times until the color of the fat meets the requirement, and drying the fat;
s9, quantitatively analyzing the purified fat by adopting an analysis method;
s10, detecting fat inhibition effect.
2. The method for detecting fat suppression effect according to claim 1, wherein in S1, a sample to be detected is obtained, the sample is weighed, and the weighing data is recorded.
3. The method for detecting fat suppression effect according to claim 1, wherein in S2, the sample is placed in a grinding device, the speed of the grinding device is controlled to 60-80r/min, and the sample is ground for 25-30min.
4. The method for detecting fat suppression effect according to claim 1, wherein in S3, the ground sample is taken out and packaged in 3-5 groups.
5. The method for detecting fat suppression effect according to claim 1, wherein the step of extracting fat in S4 comprises the specific steps of: firstly placing a sample in an extraction container, adding an organic solvent into the container, and extracting fat in the container, wherein the organic solvent is one or more of diethyl ether and petroleum ether.
6. The method for detecting fat suppression effect according to claim 1, wherein in S5, a suitable extraction method is selected for fat extraction, and the extraction method is one of a soxhlet extraction method and an ultrasonic-assisted extraction method.
7. The method for detecting fat suppression effect according to claim 1, wherein in S6, fat is placed in a decolorizer, a proper amount of adsorbent is added, and the mixture is sufficiently stirred to make the adsorbent fully contact with the fat, and pigment and impurities therein are adsorbed, and the adsorbent is one or more of activated carbon and silica gel.
8. The method for detecting fat suppression effect according to claim 1, wherein in S7, the fat is dried after the adsorbent is sufficiently adsorbed and the adsorbent is filtered out after the rest is performed for 20 to 25 minutes.
9. The method for detecting fat suppression effect according to claim 1, wherein in S9, the purified fat is quantitatively analyzed by an analysis method, and the analysis method is one of gas chromatography and high performance liquid chromatography.
10. The method for detecting a fat suppression effect according to claim 1, wherein in S10, the fat suppression effect is detected by comparing the fat content changes before and after the sample treatment. If the fat content of the sample after treatment is reduced, the fat inhibition effect is better; if the fat content after the sample treatment is increased, it is indicated that the fat-suppression effect is poor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311822268.9A CN117783385A (en) | 2023-12-27 | 2023-12-27 | Method for detecting fat inhibition effect |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311822268.9A CN117783385A (en) | 2023-12-27 | 2023-12-27 | Method for detecting fat inhibition effect |
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| Publication Number | Publication Date |
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| CN117783385A true CN117783385A (en) | 2024-03-29 |
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| CN202311822268.9A Pending CN117783385A (en) | 2023-12-27 | 2023-12-27 | Method for detecting fat inhibition effect |
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| Country | Link |
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| CN (1) | CN117783385A (en) |
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- 2023-12-27 CN CN202311822268.9A patent/CN117783385A/en active Pending
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