CN117774310A

CN117774310A - Medical organization 3D printer

Info

Publication number: CN117774310A
Application number: CN202410155916.8A
Authority: CN
Inventors: 刘超男; 刘言琳
Original assignee: Jiuhe Pharmaceutical Industry Jiangsu Co ltd
Current assignee: Jiuhe Pharmaceutical Industry Jiangsu Co ltd
Priority date: 2024-02-04
Filing date: 2024-02-04
Publication date: 2024-03-29

Abstract

The invention belongs to the technical field of medical 3D printing, and particularly relates to a medical tissue 3D printer which comprises a printing main body, a printing driving mechanism, a spraying type anti-interference cell protection assembly and a mechanical penetration type sound wave stimulation mechanism, wherein the printing driving mechanism is arranged in the printing main body; the invention provides a medical tissue 3D printer, which can reduce oxidative stress and apoptosis of cells after printing through a spray type anti-interference cell protection assembly.

Description

Medical organization 3D printer

Technical Field

The invention belongs to the technical field of medical 3D printing, and particularly relates to a medical tissue 3D printer.

Background

The 3D printer prints medical tissue is a method of constructing human tissue or organ having specific morphology and function by stacking cells, biomolecules and biomaterials layer by layer according to a preset pattern using a 3D bio-printing technique.

However, printing medical tissues with 3D printers also faces problems and challenges, and during 3D bioprinting, cells are subjected to various physical, chemical and biological stimuli such as shear forces, temperature changes, nutrient deficiencies, etc., which may affect the activity and function of the cells, so how to protect the cells from damage during printing and promote proliferation, differentiation and maturation of the cells after printing is a critical issue.

Disclosure of Invention

Aiming at the situation, in order to overcome the defects of the prior art, the invention provides the medical tissue 3D printer, and the oxidative stress and apoptosis of cells after printing can be reduced by spraying the anti-interference cell protection component.

The technical scheme adopted by the invention is as follows: the invention provides a medical tissue 3D printer which comprises a printing main body, a printing driving mechanism, a spraying type anti-interference cell protection component and a mechanical penetration type sound wave stimulation mechanism, wherein the printing driving mechanism is arranged in the printing main body; the spraying type anti-interference cell protection assembly comprises a cell activity clamping assembly and a down-pressing type uniform spraying assembly, wherein the cell activity clamping assembly is arranged on the printing main body, and the down-pressing type uniform spraying assembly is arranged on the printing main body.

Further, print the main part and include base, print frame, culture dish, motor one, lead screw one, slide bar one and lifter plate, the lower extreme of printing the main part is located to the base, print the upper end of setting up the base, motor one locates upper end one side of base, the output of motor one is located to the one end of lead screw one, the other end of motor one rotates the inside upper end of locating the print frame, the inside right side upper end of print frame is located to the one end of slide bar one, the inside right side lower extreme of print frame is located to the other end of slide bar one, the left side rear end cover of lifter plate is located on the lead screw one, the right side rear end slip cup joint of lifter plate is located on the slide bar one, lifter plate and lead screw one mesh rotation link to each other, the culture dish can be dismantled and locate on the lifter plate.

Further, the cell activity adds holds subassembly and includes ink storehouse, prints shower nozzle, atomizer, connecting pipe, transfer chamber, cytoprotective agent, solvent storage chamber and booster pump, the upper end of printing the frame is located to the ink storehouse, print the shower nozzle and locate on the printing actuating mechanism, the upper end of printing the shower nozzle is located to the output of ink storehouse, the transfer chamber cup joints and locates the lateral wall lower extreme of printing the shower nozzle, the upper end of printing the frame is located to the solvent storage chamber, cytoprotective agent is removable to be located the solvent storage intracavity, the lower extreme in solvent storage chamber is located in the input link up of booster pump, the output of booster pump is located in the one end link up of connecting pipe, the other end link up of connecting pipe is located on the lateral wall of transfer chamber, the lower extreme in transfer chamber is located to the input of atomizer.

Further, the even subassembly that sprays of push down includes air pump, coupling hose, air current flow chamber and venthole, the upper end of printing the frame is located to the air pump, coupling hose's one end link up the output of locating the air pump, the air current flow chamber cup joints and locates on the transfer chamber, coupling hose's the other end link up and locate on the lateral wall of air current flow chamber, the venthole is located the lower extreme of air current flow chamber.

Further, the mechanical infiltration type acoustic wave stimulation mechanism comprises an internal substance stimulation component and a uniform infiltration component, wherein the internal substance stimulation component is arranged on the lifting plate, and the uniform infiltration component is arranged in the lifting plate.

Further, even infiltration subassembly includes annular spout, slider one, gear, tooth piece, motor two, solid fixed ring and swivel mount, annular spout is located in the lifter plate, slider one slides and locates in the annular spout, on slider one is located to the lower extreme of swivel mount, gu the fixed ring cup joints on the swivel mount, in the lifter plate was located to motor two, the output of motor two is located to the gear, tooth piece annular array is located on the solid fixed ring, gear and tooth piece meshing rotate and link to each other.

Further, the inside material stimulating assembly comprises an ultrasonic generator, a fixed rope, an atomizer, a growth factor solution, a fog outlet and a fan, wherein one end of the fixed rope is arranged at the other end of the rotating frame, the ultrasonic generator is arranged at one end of the fixed rope, the output end of the atomizer is communicated with the lower end of the culture dish, the growth factor solution is arranged in the atomizer in a replaceable manner, the fog outlet is arranged on the inner side wall of the culture dish, and the fan is arranged in the culture dish.

Further, the printing driving mechanism comprises a longitudinal adjusting assembly and a transverse adjusting assembly, wherein the longitudinal adjusting assembly is arranged in the printing frame, and the transverse adjusting assembly is arranged on the longitudinal adjusting assembly.

Further, the vertical adjustment subassembly includes motor three, lead screw two, bearing, slide bar two, mounting one and adapter sleeve, motor three locates the lateral wall rear end of printing the frame, the output of motor three is located to the one end of lead screw two, the inner wall upper end of printing the frame is located to the bearing, the other end of lead screw two is located on the bearing, the inner wall upper end opposite side of locating the printing the frame of mounting one, the inside wall rear end of printing the frame is located to the one end of slide bar two, the other end of slide bar two is located on the mounting one, adapter sleeve's one end cup joints and locates on the lead screw two, adapter sleeve's one end and lead screw two meshing rotate and link to each other, adapter sleeve's other end sliding cup joints and locates on the slide bar two.

Further, the horizontal adjustment subassembly includes mounting two, mounting three, motor four, lead screw three, slide bar three and slider two, the one end downside of adapter sleeve is located to mounting two, the other end downside of adapter sleeve is located to mounting three, on the lateral wall of mounting two was located to the one end of slide bar three, the other end of slide bar is located on the lateral wall of mounting three, on the other lateral wall of mounting three was located to motor four, the output of motor four is located to the one end of lead screw three, the other end rotation of lead screw three is located on the lateral wall of mounting two, the slider two is slided and is cup jointed on the slide bar three, the slider two is cup jointed and is located on the lead screw three, slider two and lead screw three meshing rotate and link to each other, print the shower nozzle and locate on the slider two.

The beneficial effects obtained by the invention by adopting the structure are as follows: the invention provides a medical tissue 3D printer, which has the following beneficial effects:

(1) In order to solve the problem that cells can be subjected to various physical, chemical and biological stimuli such as shearing force, temperature change, nutrition deficiency and the like in the current 3D biological printing process, and the activities and functions of the cells can be influenced by the stimuli, the invention can reduce oxidative stress and apoptosis of the cells after printing through the spray type anti-interference cell protection component, and the advantage of the mode is that the interference on the performance of the biological ink can be avoided.

(2) Through spraying type anti-interference cell protection component, the stability and the reliability of the 3D printing medical tissue can be enhanced, and adverse effects on cells caused by water evaporation, temperature change, oxidative damage and the like in the printing process are prevented.

(3) Through spraying type anti-interference cell protection component, the self-healing capability of the medical tissue can be promoted to be printed in 3D, so that the medical tissue can quickly recover the original structure and function after being cut or damaged.

(4) The spray type anti-interference cell protection component can improve the biocompatibility and biodegradability of the 3D printing medical tissue, so that the 3D printing medical tissue can be well combined and communicated with human tissue, and can be gradually absorbed or removed after treatment is finished.

(5) In order to further improve the practicability and generalizability, the invention provides a mechanical infiltration type acoustic wave stimulation mechanism, wherein the mechanical effect is utilized, the ultrasonic vibration can cause the movement of substances in cells, change the permeability of cell membranes, promote metabolism, blood circulation, tissue nutrition and the like, and improve the functions and regeneration capacity of the cells.

(6) Through the mechanical penetration type acoustic wave stimulation mechanism, the ultrasonic energy can be converted into heat energy, so that the cell temperature is increased, the enzyme activity, the protein synthesis, the gene expression and the like are enhanced, and the physiological activity of the cells is regulated.

(7) Through the mechanical penetration type acoustic wave stimulation mechanism, the ultrasonic wave can influence chemical reactions inside and outside the cell, generate free radicals, gas bubbles and the like, and change redox states, signal transduction, secretion functions and the like of the cell.

(8) Through the mechanical penetration type acoustic wave stimulation mechanism, the ultrasonic wave can stimulate specific acoustic sensitive channel proteins, so that calcium ions in cells flow, action potential is triggered, and the activities of neurons and muscle cells are regulated and controlled.

(9) Atomized growth factors can promote the exchange and permeation of intracellular and extracellular substances by increasing the permeability of cell membranes. This helps the cells to take up more nutrients and release metabolites, improving the cell's environment of survival; the proper amount of growth factors can stimulate the proliferation and growth of cells and accelerate the tissue repair and regeneration process; under the guidance of the atomized growth factors after ultrasonic stimulation, the cells can absorb the growth factors more effectively, promote the division and proliferation of the cells and accelerate the tissue repair speed.

(10) Enhancing the permeability of cell membranes by mechanical osmotic acoustic stimulation mechanisms helps to increase the efficiency of intracellular metabolism. The cells can absorb nutrient substances better and release wastes and metabolites, so that the intracellular environment is improved, the normal functions and physiological processes of the cells are promoted, and the atomized growth factors can improve the effect of drug delivery by increasing the permeability of cell membranes; the medicine can more easily penetrate through the cell membrane to enter the inside of the cell, so that more effective treatment effect is realized; the combination of ultrasonic stimulation and atomized growth factors can promote cell migration, differentiation and tissue reconstruction. The method has important significance in the fields of medical tissue engineering and regenerative medicine, and can accelerate the repair and regeneration process of damaged tissues.

Drawings

Fig. 1 is a front view of a medical tissue 3D printer according to the present invention;

fig. 2 is a front view cross-section of a medical tissue 3D printer according to the present invention;

FIG. 3 is a left side cross-sectional view of a 3D printer for medical tissue according to the present invention;

FIG. 4 is a right side cross-sectional view of a 3D printer for medical tissue according to the present invention;

FIG. 5 is a schematic diagram of a spray-type tamper-proof cytoprotective assembly;

FIG. 6 is a bottom view of the print head;

FIG. 7 is a front view of a mechanical penetration acoustic stimulation mechanism;

FIG. 8 is a front cross-sectional view of a mechanical penetration acoustic stimulation mechanism;

fig. 9 is a partial enlarged view of a portion a in fig. 8.

Wherein, 1, a printing main body, 2, a spraying type anti-interference cell protection component, 3, a mechanical infiltration type acoustic wave stimulation mechanism, 4, a printing driving mechanism, 5, a base, 6, a printing frame, 7, a culture dish, 8, a motor I, 9, a screw I, 10, a slide bar I, 11, a lifting plate, 12, a cell activity adding and holding component, 13, a down-pressing type uniform spraying component, 14, an ink bin, 15, a printing spray head, 16, an atomization spray head, 17, a connecting pipe, 18, a transfer cavity, 19, a cell protective agent, 20, a solvent storage cavity, 21, a booster pump, 22, an air pump, 23, a connecting hose, 24, an air flow cavity, 25 and an air outlet, 26, an internal substance stimulating assembly, 27, a uniform penetration assembly, 28, an ultrasonic generator, 29, a fixing rope, 30, an atomizer, 31, a growth factor solution, 32, a mist outlet, 33, a fan, 34, an annular chute, 35, a first slider, 36, a gear, 37, a tooth block, 38, a second motor, 39, a longitudinal adjustment assembly, 40, a transverse adjustment assembly, 41, a third motor, 42, a second screw, 43, a bearing, 44, a second slide bar, 45, a first fixing piece, 46, a connecting sleeve, 47, a second fixing piece, 48, a third fixing piece, 49, a fourth motor, 50, a third screw, 51, a third slide bar, 52, a second slider, 53, a fixing ring, 54 and a rotating frame.

The accompanying drawings are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate the invention and together with the embodiments of the invention, serve to explain the invention.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention; all other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

In the description of the present invention, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," and the like indicate orientation or positional relationships based on those shown in the drawings, merely to facilitate description of the invention and simplify the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the invention.

As shown in fig. 1-9, the invention provides a medical tissue 3D printer, which comprises a printing main body 1, a printing driving mechanism 4, a spraying type anti-interference cell protection component 2 and a mechanical penetration type sound wave stimulation mechanism 3, wherein the printing driving mechanism 4 is arranged in the printing main body 1, the mechanical penetration type sound wave stimulation mechanism 3 is arranged in the printing main body 1, and the spraying type anti-interference cell protection component 2 is arranged on the printing main body 1; the spraying type anti-interference cell protection assembly 2 comprises a cell activity clamping assembly 12 and a down-pressing type uniform spraying assembly 13, wherein the cell activity clamping assembly 12 is arranged on the printing main body 1, and the down-pressing type uniform spraying assembly 13 is arranged on the printing main body 1.

The printing main body 1 comprises a base 5, a printing frame 6, a culture dish 7, a motor 8, a screw rod 9, a slide rod 10 and a lifting plate 11, wherein the base 5 is arranged at the lower end of the printing main body 1, the printing frame 6 is arranged at the upper end of the base 5, the motor 8 is arranged at one side of the upper end of the base 5, one end of the screw rod 9 is arranged at the output end of the motor 8, the other end of the motor 8 is rotationally arranged at the inner upper end of the printing frame 6, one end of the slide rod 10 is arranged at the inner right upper end of the printing frame 6, the other end of the slide rod 10 is arranged at the inner right lower end of the printing frame 6, the left rear end of the lifting plate 11 is sleeved on the screw rod 9 in a sleeved mode, the right rear end of the lifting plate 11 is sleeved on the slide rod 10 in a sliding mode, the lifting plate 11 is meshed and rotationally connected with the screw rod 9, and the culture dish 7 is detachably arranged on the lifting plate 11.

The mechanical penetration type acoustic wave stimulation mechanism 3 comprises an internal substance stimulation component 26 and a uniform penetration component 27, wherein the internal substance stimulation component 26 is arranged on the lifting plate 11, and the uniform penetration component 27 is arranged in the lifting plate 11.

The uniform permeation assembly 27 comprises an annular chute 34, a first slide block 35, a gear 36, a tooth block 37, a second motor 38, a fixed ring 53 and a rotating frame 54, wherein the annular chute 34 is arranged in the lifting plate 11, the first slide block 35 is arranged in the annular chute 34 in a sliding mode, the lower end of the rotating frame 54 is arranged on the first slide block 35, the fixed ring 53 is sleeved on the rotating frame 54, the second motor 38 is arranged in the lifting plate 11, the gear 36 is arranged at the output end of the second motor 38, the tooth block 37 is arranged on the fixed ring 53 in an annular array mode, and the gear 36 is meshed with the tooth block 37 to rotate and be connected.

The internal substance stimulating assembly 26 includes an ultrasonic generator 28, a fixing rope 29, an atomizer 30, a growth factor solution 31, a mist outlet 32 and a fan 33, wherein one end of the fixing rope 29 is arranged at the other end of the rotating frame 54, the ultrasonic generator 28 is arranged at one end of the fixing rope 29, the output end of the atomizer 30 is arranged at the lower end of the culture dish 7 in a penetrating manner, the growth factor solution 31 is arranged in the atomizer 30 in a replaceable manner, the mist outlet 32 is arranged on the inner side wall of the culture dish 7, and the fan 33 is arranged in the culture dish 7.

The printing driving mechanism 4 comprises a longitudinal adjustment assembly 39 and a transverse adjustment assembly 40, wherein the longitudinal adjustment assembly 39 is arranged in the printing frame 6, and the transverse adjustment assembly 40 is arranged on the longitudinal adjustment assembly 39.

The longitudinal adjustment assembly 39 comprises a motor III 41, a screw rod II 42, a bearing 43, a slide rod II 44, a fixing piece I45 and a connecting sleeve 46, wherein the motor III 41 is arranged at the rear end of the outer side wall of the printing frame 6, one end of the screw rod II 42 is arranged at the output end of the motor III 41, the bearing 43 is arranged at the upper end of the inner wall of the printing frame 6, the other end of the screw rod II 42 is arranged on the bearing 43, one end of the fixing piece I45 is arranged at the other side of the upper end of the inner wall of the printing frame 6, one end of the slide rod II 44 is arranged at the rear end of the inner side wall of the printing frame 6, the other end of the slide rod II 44 is arranged on the fixing piece I45, one end of the connecting sleeve 46 is sleeved on the screw rod II 42, one end of the connecting sleeve 46 is meshed and connected with the screw rod II 42 in a rotating manner, and the other end of the connecting sleeve 46 is sleeved on the slide rod II 44 in a sliding manner.

The transverse adjusting assembly 40 comprises a second fixing piece 47, a third fixing piece 48, a fourth motor 49, a third screw rod 50, a third sliding rod 51 and a second sliding block 52, the second fixing piece 47 is arranged on the lower side of one end of the connecting sleeve 46, the third fixing piece 48 is arranged on the lower side of the other end of the connecting sleeve 46, one end of the third sliding rod 51 is arranged on the side wall of the second fixing piece 47, the other end of the third sliding rod 51 is arranged on the side wall of the third fixing piece 48, the fourth motor 49 is arranged on the other side wall of the third fixing piece 48, one end of the third screw rod 50 is arranged at the output end of the fourth motor 49, the other end of the third screw rod 50 is rotationally arranged on the side wall of the second fixing piece 47, the second sliding block 52 is slidingly sleeved on the third sliding rod 51, the second sliding block 52 is sleeved on the third screw rod 50, the second sliding block 52 is rotationally connected with the third screw rod 50 in a meshed mode, and the printing nozzle 15 is arranged on the second sliding block 52.

The cell activity adds holds subassembly 12 and includes ink storehouse 14, print shower nozzle 15, atomizer 16, connecting pipe 17, transfer chamber 18, cytoprotective agent 19, solvent storage chamber 20 and booster pump 21, the upper end of printing frame 6 is located to ink storehouse 14, print shower nozzle 15 is located on printing actuating mechanism 4, the upper end of printing shower nozzle 15 is located to the output of ink storehouse 14, transfer chamber 18 cup joints the lateral wall lower extreme of printing shower nozzle 15, solvent storage chamber 20 is located the upper end of printing frame 6, cytoprotective agent 19 is removable locate in solvent storage chamber 20, the lower extreme in solvent storage chamber 20 is located in the input link up of booster pump 21, the output of booster pump 21 is located in the link up of connecting pipe 17, the other end link up of connecting pipe 17 is located on the lateral wall of transfer chamber 18, the lower extreme in transfer chamber 18 is located to atomizer 16's input.

The down-pressure type uniform spraying assembly 13 comprises an air pump 22, a connecting hose 23, an air flow cavity 24 and an air outlet hole 25, wherein the air pump 22 is arranged at the upper end of the printing frame 6, one end of the connecting hose 23 is communicated with the output end of the air pump 22, the air flow cavity 24 is sleeved on the transfer cavity 18, the other end of the connecting hose 23 is communicated with the side wall of the air flow cavity 24, and the air outlet hole 25 is arranged at the lower end of the air flow cavity 24.

When the biological ink is specifically used, firstly, the cultured biological ink is put into the ink bin 14, the output end of the motor I8 rotates to drive the screw rod I9 to rotate, the screw rod I9 rotates to drive the lifting plate 11 to move up and down, the lifting plate 11 moves up and down to drive the culture dish 7 to move up and down, the output end of the motor III 41 rotates to drive the screw rod II 42 to rotate, the screw rod II 42 rotates to drive the connecting sleeve 46 to move back and forth, the connecting sleeve 46 moves back and forth to drive the transverse adjusting assembly 40 to move back and forth, the transverse adjusting assembly 40 moves back and forth to drive the printing spray head 15, the output end of the motor IV 49 rotates to drive the screw rod III 50 to rotate, the screw rod III 50 rotates to drive the slide block II 52 to move left and right, the slide block II 52 moves left and right to drive the printing spray head 15 to move left and right, thereby medical tissues are printed in the culture dish 7, when the medical tissues are printed, the booster pump 21 is started to input the cytoprotective agent 19 in the solvent storage cavity 20 into the transfer cavity 18 through the connecting pipe 17, finally, the air is sprayed out through the atomizing nozzle 16 so as to reduce oxidative stress and apoptosis of cells after printing, the method has the advantages that interference to the performance of biological ink can be avoided, the air pump 22 is started, air is conveyed into the air flow cavity 24 through the connecting hose 23, finally, the air is sprayed out through the air outlet 25, the auxiliary cell protectant 19 is sprayed on medical tissues, so that the distribution of the cell protectant 19 and the cells is more uniform, the stability and the reliability of printing the medical tissues can be enhanced by spraying the cell protectant 19, adverse effects on the cells caused by water evaporation, temperature change, oxidative damage and the like in the printing process are prevented, the self-healing capacity of the printing medical tissues can be promoted by spraying the cell protectant 19, the original structure and function can be quickly recovered after the printing medical tissues are cut or damaged, spraying the cytoprotective agent 19 can improve the biocompatibility and biodegradability of the printed medical tissue, so that the printed medical tissue can be well combined and communicated with human tissue, and can be gradually absorbed or removed after treatment is completed, after printing is completed, the atomizer 30 is started to atomize the growth factor solution 31 into the culture dish 7, the atomized growth factor solution 31 is sprayed out through the mist outlet 32 by the fan 33, the ultrasonic generator 28 is started to generate ultrasonic waves, the output end of the motor II 38 rotates to drive the gear 36 to rotate, the gear 36 rotates to drive the tooth block 37 to rotate, the tooth block 37 rotates to drive the fixed ring 53 to rotate, the fixed ring 53 rotates to drive the rotating frame 54 to rotate, the rotating frame 54 rotates to drive the fixed rope 29 to rotate, the fixed rope 29 rotates to drive the internal substance stimulating assembly 26 to rotate, the permeability of cell membranes is stimulated by the ultrasonic waves, the ultrasonic waves can cause the movement of substances in cells, changing permeability of cell membrane, promoting metabolism, blood circulation, tissue nutrition, etc., improving cell function and regeneration capability, converting ultrasonic energy into heat energy, increasing cell temperature, enhancing enzyme activity, protein synthesis, gene expression, etc., regulating physiological activity of cell, ultrasonic wave can influence chemical reaction inside and outside cell, generating free radical, gas bubble, etc., changing redox state, signal transduction, secretion function, etc., ultrasonic wave can stimulate specific sound-sensitive channel protein, make intracellular calcium ion flow, initiate action potential, regulate activity of neuron and muscle cell, stimulating cell membrane permeability by ultrasonic wave with atomized growth factor, and promoting exchange and permeation of substances inside and outside cell by increasing cell membrane permeability, the method is favorable for cells to absorb more nutrients and release metabolites, the living environment of the cells is improved, a proper amount of growth factors can stimulate proliferation and growth of the cells, tissue repair and regeneration processes are accelerated, the cells can absorb the growth factors more effectively under the guidance of the atomized growth factors after ultrasonic stimulation, cell division and proliferation are promoted, tissue repair speed is accelerated, cell membrane permeability is enhanced, the efficiency of intracellular metabolism is improved, the cells can absorb the nutrients better, waste and metabolites are released, thereby improving the intracellular environment, normal functions and physiological processes of the cells are promoted, the permeability of the cell membranes is increased, the effect of drug delivery can be improved, the drugs can penetrate the cell membranes more easily into the cells, more effective treatment effect is realized, ultrasonic stimulation and atomized growth factors are combined, migration, differentiation and tissue reconstruction of the cells are promoted, the method has important significance for the medical tissue engineering and regeneration medical field, the effect of repairing and regeneration of damaged tissues can be accelerated, the effect of the fixing rope 29 is reduced, the influence of the internal substance stimulation component 26 on the culture dish 7 is reduced, and the method can be repeatedly used in the next working process.

It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

The invention and its embodiments have been described above with no limitation, and the actual construction is not limited to the embodiments of the invention as shown in the drawings. In summary, if one of ordinary skill in the art is informed by this disclosure, a structural manner and an embodiment similar to the technical solution should not be creatively devised without departing from the gist of the present invention.

Claims

1. The utility model provides a medical tissue 3D printer, includes printing main part (1) and prints actuating mechanism (4), its characterized in that: the medical tissue 3D printer further comprises a spraying type anti-interference cell protection assembly (2) and a mechanical infiltration type sound wave stimulation mechanism (3), wherein the printing driving mechanism (4) is arranged in the printing main body (1), the mechanical infiltration type sound wave stimulation mechanism (3) is arranged in the printing main body (1), and the spraying type anti-interference cell protection assembly (2) is arranged on the printing main body (1); the spraying type anti-interference cell protection assembly (2) comprises a cell activity clamping assembly (12) and a down-pressing type uniform spraying assembly (13), wherein the cell activity clamping assembly (12) is arranged on the printing main body (1), and the down-pressing type uniform spraying assembly (13) is arranged on the printing main body (1).

2. A medical tissue 3D printer according to claim 1, wherein: the printing main body (1) comprises a base (5), a printing frame (6), a culture dish (7), a motor (8), a screw rod (9), a slide rod (10) and a lifting plate (11), wherein the base (5) is arranged at the lower end of the printing main body (1), the printing frame (6) is arranged at the upper end of the base (5), the motor (8) is arranged at one side of the upper end of the base (5), one end of the screw rod (9) is arranged at the output end of the motor (8), the other end of the motor (8) is rotationally arranged at the upper end of the inside of the printing frame (6), one end of the slide rod (10) is arranged at the upper end of the right side of the inside of the printing frame (6), the other end of the slide rod (10) is arranged at the lower end of the right side of the inside of the printing frame (6), the left rear end of the lifting plate (11) is sleeved on the screw rod (9), the right rear end of the lifting plate (11) is slidingly sleeved on the lifting plate (10), the lifting plate (11) is rotationally connected with the screw rod (9), and the lifting plate (11) is meshed with the screw rod (7).

3. A medical tissue 3D printer according to claim 2, wherein: the cell activity adds and holds subassembly (12) including ink storehouse (14), print shower nozzle (15), atomizer (16), connecting pipe (17), transfer chamber (18), cytoprotective agent (19), solvent storage chamber (20) and booster pump (21), the upper end of printing frame (6) is located in ink storehouse (14), print shower nozzle (15) are located on printing actuating mechanism (4), the upper end of printing shower nozzle (15) is located to the output of ink storehouse (14), the lateral wall lower extreme of printing shower nozzle (15) is located in transfer chamber (18) cup joint, the upper end of printing frame (6) is located in solvent storage chamber (20), cytoprotective agent (19) are removable to be located in solvent storage chamber (20), the lower extreme of locating solvent storage chamber (20) is link up to the input of booster pump (21), the output of booster pump (21) is link up to the one end of connecting pipe (17), the other end of link up on locating the lateral wall of transfer chamber (18), the lower extreme of locating transfer chamber (18) is located to the input of atomizer (16).

4. A medical tissue 3D printer according to claim 3, wherein: the down-pressure type uniform spraying assembly (13) comprises an air pump (22), a connecting hose (23), an airflow flowing cavity (24) and an air outlet (25), wherein the air pump (22) is arranged at the upper end of the printing frame (6), one end of the connecting hose (23) is communicated with the output end of the air pump (22), the airflow flowing cavity (24) is sleeved on the transferring cavity (18), the other end of the connecting hose (23) is communicated with the side wall of the airflow flowing cavity (24), and the air outlet (25) is arranged at the lower end of the airflow flowing cavity (24).

5. The medical tissue 3D printer of claim 4, wherein: the mechanical infiltration type acoustic wave stimulation mechanism (3) comprises an internal substance stimulation component (26) and a uniform infiltration component (27), wherein the internal substance stimulation component (26) is arranged on the lifting plate (11), and the uniform infiltration component (27) is arranged in the lifting plate (11).

6. The medical tissue 3D printer of claim 5, wherein: the utility model provides a evenly permeate subassembly (27) includes annular spout (34), slider one (35), gear (36), tooth piece (37), motor two (38), solid fixed ring (53) and swivel mount (54), in lifting plate (11) are located to annular spout (34), slider one (35) are slided and are located in annular spout (34), on slider one (35) are located to the lower extreme of swivel mount (54), gu fixed ring (53) cup joint and locate on swivel mount (54), in lifting plate (11) are located to motor two (38), the output of motor two (38) is located to gear (36), tooth piece (37) annular array is located on solid fixed ring (53), gear (36) and tooth piece (37) meshing rotate and link to each other.

7. The medical tissue 3D printer of claim 6, wherein: the utility model provides an inside material stimulating assembly (26) includes supersonic generator (28), fixed rope (29), atomizer (30), growth factor solution (31), goes out fog hole (32) and fan (33), the other end of swivel mount (54) is located to the one end of fixed rope (29), supersonic generator (28) are located the one end of fixed rope (29), the lower extreme of culture dish (7) is located in the link up of output of atomizer (30), growth factor solution (31) are removable locates in atomizer (30), go out fog hole (32) are located on the inside wall of culture dish (7), in culture dish (7) is located to fan (33).

8. The medical tissue 3D printer of claim 7, wherein: the printing driving mechanism (4) comprises a longitudinal adjusting component (39) and a transverse adjusting component (40), the longitudinal adjusting component (39) is arranged in the printing frame (6), and the transverse adjusting component (40) is arranged on the longitudinal adjusting component (39).

9. The medical tissue 3D printer of claim 8, wherein: the vertical adjustment subassembly (39) includes motor three (41), lead screw two (42), bearing (43), slide bar two (44), mounting one (45) and adapter sleeve (46), the lateral wall rear end of printing frame (6) is located to motor three (41), the output of motor three (41) is located to the one end of lead screw two (42), the inner wall upper end of printing frame (6) is located to bearing (43), the other end of lead screw two (42) is located on bearing (43), the inner wall upper end opposite side of printing frame (6) is located to the one end of mounting one (45), the inside wall rear end of printing frame (6) is located to the one end of slide bar two (44), the other end of slide bar two (44) is located on mounting one (45), the one end of adapter sleeve (46) is cup jointed and is located on lead screw two (42), the one end of adapter sleeve (46) and lead screw two (42) mesh and rotate and link, the other end of adapter sleeve (46) is slidingly cup jointed and is located on slide bar two (44).

10. A medical tissue 3D printer according to claim 9, wherein: the horizontal adjustment assembly (40) comprises a second fixing part (47), a third fixing part (48), a fourth motor (49), a third screw rod (50), a third sliding rod (51) and a second sliding block (52), wherein the second fixing part (47) is arranged at the lower side of one end of the second fixing part (46), the third fixing part (48) is arranged at the lower side of the other end of the second connecting part (46), one end of the third sliding rod (51) is arranged on the side wall of the second fixing part (47), the other end of the third sliding rod (51) is arranged on the side wall of the third fixing part (48), the fourth motor (49) is arranged on the other side wall of the third fixing part (48), one end of the third screw rod (50) is arranged at the output end of the fourth motor (49), the other end of the third screw rod (50) is rotationally arranged on the side wall of the second fixing part (47), the second sliding block (52) is slidingly sleeved on the third sliding rod (51), the second sliding block (52) is arranged on the third sliding rod (50), the second sliding block (52) is meshed with the third sliding rod (50) and is rotationally connected, and the second sliding block (52) is meshed with the third sliding rod (50).