CN117771278A

CN117771278A - Application of Prevotella copri in preparation of medicine for treating senile debilitation syndrome

Info

Publication number: CN117771278A
Application number: CN202410208531.3A
Authority: CN
Inventors: 刘晓蕾; 董碧蓉; 岳冀蓉; 李代萍; 胡凤娟; 唐静宜; 许志刚
Original assignee: West China Hospital of Sichuan University
Current assignee: West China Hospital of Sichuan University
Priority date: 2024-02-26
Filing date: 2024-02-26
Publication date: 2024-03-29

Abstract

The invention relates to an application of Prevotella copri in preparing a medicament for treating senile debilitating syndrome, belonging to the field of medicaments. The Prevotella copri disclosed by the invention is used for treating the senile debilitating syndrome, has definite efficacy, belongs to probiotics, is safe in clinical use, and has the advantages of high practicality and wide application range, and the gap of treatment of intestinal flora of the senile debilitating syndrome is filled.

Description

Application of Prevotella copri in preparation of medicine for treating senile debilitation syndrome

Technical Field

The invention relates to an application of Prevotella copri in preparing a medicament for treating senile debilitating syndrome, belonging to the field of medicaments.

Background

Debilitation (Frailty) is a common senile syndrome, and refers to a nonspecific state in which the physiological reserve of the elderly is reduced, resulting in increased vulnerability of the body and reduced anti-stress ability. The debilitating elderly experience less external stimulus, which results in a series of clinical negative events. The diagnosis standard which is widely used at present for the weakness is Fried and the like, and comprises muscle strength reduction, energy exhaustion, slow pace, activity endurance reduction and purposeless weight reduction, and three or more of the symptoms are satisfied. The prevalence of debilitation increases with age, women are higher than men, and medical institutions are higher than communities. The study in the United states shows that the incidence of weakness over 65 years old is 7% -12% and the incidence of weakness over 80 years old is up to one third of the aged population in the community. The debilitation causes disability and functional decline of the old, leads to increase of hospitalization rate and death rate, and greatly increases the requirement of the old on long-term care and medical cost. The present studies have found that genetic factors, age-related, poor economic conditions, low educational level, poor lifestyle, senile syndrome (falls, sarcopenia, malnutrition), married, solitary, etc. are all debilitating risk factors [ wo J, chan R, leung J, et al Relative contributions of geographic, sonoeconic, and lifestyle factors to quality of life, frailty, and mortality in elderly [ J ], PLoS One, 2010, 5 (1): e8775 ].

The pathogenesis of debilitation is currently not well defined, where genetic polymorphisms may affect debilitating clinical phenotypes, and research has found that the debilitation rate of african americans is 4 times that of other americans, and the debilitation prevalence of mexico americans is 4.3% higher than that of african americans. Wherein the apolipoprotein E (APOE) gene, insulin receptor-like gene-2 (DAF-2), C-reactive protein coding region (CRP 1846G > A), muscle cell mitochondrial DNA (mt 204C), interleukin (IL-6) and the like may all be associated with debilitation [ Vina J, tarazona-Santaballbina FJ, perez-Ros P, et al Biology of frailty: modulation of ageing genes and its importance to prevent age-associated loss of function [ J ]. Mol peaks Med, 2016, 50 (4): 88-108 ]. Inflammatory aging caused by chronic inflammation plays an important role in debilitation [ Chen X, mao G, leng SX. Frailty syndrome: an overview [ J ]. Clin Interv Aging, 2014, 9:433-441 ], numerous studies indicate that the elderly population may exhibit chronic inflammatory states, manifested by elevated inflammatory molecules such as interleukin (IL-6), C-reactive protein (CRP) and tumor necrosis factor-alpha (TNF-alpha) and increased numbers of leukocytes [ Leng S, chaves P, koeneng K, et al Serum interleukin-6 and hemoglobin as physiological correlates in the geriatric syndrome of Frailty: a small size student [ J ]. J Am Geriatr Soc, 2002, 50:1268-1271 ]. While Growth Hormone (GH) -IGF-1 has abnormal growth axis, hypothalamic-pituitary-adrenal axis and other hormones may also be involved in debilitating pathological processes [ Maggio M, cappola AR, ceda GP, et al The hormonal pathway to frailty in older men [ J ]. J Endocrinol Invest, 2005, 28:15-19 ]. Recent studies have found that the aging process is closely related to intestinal flora, with reduced flora stability in the elderly [ Biagi E, candela M, fairweather-Tai S, et al Ageing of the human metaorganism: the microbial counterpart [ J ]. Age, 2012; 34 (1): 247-67 ], with reduced diversity in the core intestinal flora of elderly patients such as Propionibacterium, bacteroides [ O' Toole PW, jeffery IB. Gut microbiota and aging [ J ]. Science, 2015; 350 (6265): 1214-5 ], jackson MA, jeffery IB, beaumont M, et al Signatures of early frailty in the gut microbiota [ J ]. Genome Med, 2016; 8 (1): 81-91 ]. Aging has been found to enhance the ability of the intestinal flora to catabolize proteins and fats, and bacteroides and clostridia to break down protein spoilage into deleterious ammonia, amines, phenols, indoles, which produce endotoxin, low inflammation [ Lynch DB, jeffery IB, O' Toole PW, et al jet-micro-a-health interactions in older subjects: implications for healthy aging [ J ]. Interdiscipl Top Gerontol, 2015; 40 (44): 141-54 ], and reduced digestion and breakdown of carbohydrates, which can be fermented by the intestinal flora such as clostridium tender to produce short chain fatty acids such as butyric acid which can act as an energy source for intestinal epithelial cells and have anti-inflammatory activity [ Wu GD, chen J, hoffn C, et al, link long-term dietary patterns with gut microbial enterotypes [ J ]. Pace, 2011; 334 (6052): 105-8 ].

Prevoltella copri is a strain of the genus Proteus. The current knowledge of the bacteria is not sufficient due to the difficulty in culturing and the non-pathogenicity of the bacteria. The genus Pu's bacteria is one of three large intestine representative bacteria of human intestinal microorganisms, and is also one of core bacteria of human intestinal microorganisms. Several studies have shown thatPrevotellaGenus or group ofP.copriIs positively correlated with diseases such as insulin resistance, rheumatoid arthritis and hypertension, and is negatively correlated with dyskinesia Parkinson's disease and autism [ Ley Ruth E, gut microbiota in 2015: prevotella in the gut: choose carbunclly ]]Nat Rev Gastroenterol Hepatol, 2016, 13:69-70. However, there are many shortfalls or even gaps in basic biological studies of such important intestinal microorganisms. Recently, the research shows that compared with the control group, the intestinal flora level of inflammatory bowel disease patients is equal to that of the Pu' er bacteriaP.copri) Obviously reduce the faecal bacillus of Pu' erF.prausnitzii) And (3) remarkable enrichment. Several studies have shown that Propionibacterium plays a beneficial role [ Iljazovic A, roy U, G.ivez EJC, lesker TR, zhao B, gronow A, amp L, will SE, hofmann JD, pils MC, schmidt-Hohagen K, neumann-Schaal M, streowig T. Perturbation of the gut microbiome by Prevotella spp. enhances host susceptibility to Mucosal Inflatation, mucosal immunol.2021 Jan;14 (1): 113-124 ]. Studies have shown that the isolation of Proprietaria from commensal bacteria in the human gut reduces the severity of collagen-induced arthritis in HLA-DQ8 mice [ Randins TM, ratner AJ. Gardnerella and Prevotella: co-conspirators in the Pathogenesis of Bacterial Vaginosis. J. Infect Dis. 2019;220 (7): 1085-1088. Doi:10.1093/infdis/jiy705 ]. Proprietaria inhibits serum levels of several pro-inflammatory cytokines (e.g., IL-2, IL-17 and TNF- α). Mice transplanted with praecox showed an increase in regulatory T cells in the gut and a decrease in antigen-specific Th17 responses. Whereas The enzymes secreted by Proprietas promote The fermentation of food to produce short chain fatty acids, mainly comprising butyric and propionic acids, wherein Proprietas is a butyrate producer, known for its anti-inflammatory properties [ Arweiler NB, neuschil. The Ora ]Microbiota, adv Exp Med biol 2016;902:45-60. Doi 10.1007/978-3-319-31248-4_4. PMID 27161350 ]. Short chain fatty acids are not only the primary nutrients for intestinal epithelial cells, but also modulate immune responses by activating G-protein coupled receptors and inhibiting histone deacetylases, and inhibit the expression of part of inflammatory factors, which are involved in the control of inflammatory diseases. Can also bind to fatty acid receptors 2 and 3 of skeletal muscle cells, target muscle mitochondria, promote mitochondrial biosynthesis [ Si J, you HJ, yu J, sung J, ko G. Prevotella as a Hub for Vaginal Microbiota under the Influence of Host Genetics and Their Association with Obenity, cell Host micro-be.2017 Jan 11;21 (1): 97-105 ]. Furthermore, fielding et al performed two intestinal flora tests on elderly persons having different somatic functions at one month intervals, and found that there was a stable rise in the intestinal flora of elderly persons having SPPB judged to be highly somatic in the genera Proteus and Proteus [ Fielding R A, reeves A R, jasuja R, et al Muscle strength is increased in mice that are colonized with microbiota from high-functioning older adults [ J ]]Exp Gerontol, 2019, 127: 110722. After transplanting intestinal flora of two groups of old people to mice, the abundance of Pu 'er bacteria family and Pu' er bacteria genus in the intestinal tract of the mice transplanted with high somatic function group feces is increased, and the grip strength is obviously increased by 6.4% [ Van Tongeren S P, sleets J P, harmsen H J, et al Fecal microbiota composition and frailty [ J ]]Applied and environmental microbiology, 2005, 71 (10): 6438-6442. Significant reduction of the genus Propionibacterium was also found in the stool of debilitating elderly [ Xu Y, wang Y, li H, et al Altered Fecal Microbiota Composition in Older Adults With Frailty [ J]. Front Cell Infect Microbiol, 2021, 11: 696186.，Everard A, Lazarevic V, Derrien M, et al. Responses of gut microbiota and glucose and lipid metabolism to prebiotics in genetic obese and diet-induced leptin-resistant mice [J]Diabetes, 2011, 60 (11): 2775-2786. Animal studies have found that feeding prebiotics can result in increased abundance of Pu 'er bacteria and Pu' er bacteria in the gut of obese mice with increased muscle mass or decreased fat mass [ Pisanu S, palmas V, madau V, et a ]l. Impact of a Moderately Hypocaloric Mediterranean Diet on the Gut Microbiota Composition of Italian Obese Patients [J]Nutrients, 2020, 12 (9). In the Pisanu et al study, moderately low calorie Mediterranean dietary interventions were performed on obese and overweight subjects, resulting in increased abundance of Protopanax in the gut and decreased body fat mass, but no significant effect on muscle mass [ Chen C, fang S, wei H, et al Prevotella copri increases fat accumulation in pigs fed with formula diet, microbiome. Aug 21 2021;9 (1): 175 ]. Furthermore chen et al found that the abundance of praecox in the intestinal flora was positively correlated with the concentration of obesity-related metabolites such as lipopolysaccharide, branched chain amino acids, aromatic amino acids and metabolites in the serum of pigs fed the formula. They further found, using a sterile mouse model, that the fixation of the pralidoxime bacteria activates the host chronic inflammatory response through TLR4 and mTOR signaling pathways, indicating that the pralidoxime bacteria significantly up-regulate the expression of genes associated with adipogenesis and fat accumulation. CN201810669508.9, name: the application of the Ackermana myxobacteria or the Proprietaria in preparing the medicine for enhancing the anti-tumor immunity function provides the application of the Ackermana myxobacteria or the Proprietaria in preparing the medicine for enhancing the anti-tumor immunity function of CD4+ T cells and/or CD8+ T cells and/or enhancing the anti-tumor immunity function of CD8+ T cells in the tumor microenvironment. The akaman myxobacteria or the praecox bacteria can obviously inhibit the expression of PD-1 by the systemic CD4+ T cells and/or CD8+ T cells, can promote the infiltration and/or accumulation of the CD8+ T cells in the tumor microenvironment, inhibit the expression of PD-1 molecules and obviously inhibit the growth of tumors. CN201480043869.2, name: treatment of obesity, metabolic syndrome, type 2 diabetes, cardiovascular diseases, dementia, alzheimer's disease and inflammatory bowel disease by using at least one bacterial strain from the genus Prevotella, to a product for the treatment of obesity, metabolic syndrome, type 2 diabetes, cardiovascular diseases, dementia, alzheimer's disease and inflammatory bowel disease comprising at least one isolated bacterial strain from the species Prevotella, wherein the strain is selected from the group consisting of Prevotella enterica, prevotella stercorea, prevotella histicola, prevotella ruminalis, prevotella bruceiiThe bacteria 25A and Prevotella dirachta.

There is no study of praecox and debilitating syndrome.

Disclosure of Invention

The invention provides a new application of Prevotella copri, in particular to an application in preparing a medicament for treating senile debilitation syndrome

The invention provides an application of Prevotella copri in preparing a medicament for treating senile debilitating syndrome.

Wherein the Prevotella copri exists as a living bacterium.

Wherein the medicine is a preparation prepared by taking Prevotella copri as an active ingredient and adding pharmaceutically acceptable auxiliary materials or auxiliary ingredients.

Wherein the preparation is an oral preparation.

Wherein the oral preparation is tablet, capsule, pill, granule.

The number of viable bacteria containing Prevotella copri per unit preparation in the oral preparation is not less than 1.0 x 10 ⁸ CFU. The preparation per unit refers to each tablet, each capsule, each pill and each granule.

The beneficial effects of the invention are as follows:

the Prevotella copri disclosed by the invention is used for treating the senile debilitating syndrome, has definite efficacy, belongs to probiotics, is safe in clinical use, and has the advantages of high practicality and wide application range, and the gap of treatment of intestinal flora of the senile debilitating syndrome is filled.

Drawings

FIG. 1 is a diagram of an animal behavioral baseline detection result (wherein, the diagram a is weight, the diagram b is iron wire hanging time, the diagram c is number of turns of a rotating rod, the diagram d is rotating rod speed, the diagram e is rotating rod time, the diagram f is cage top hanging time, the diagram g is forelimb holding power, the diagram h is hindlimb holding power; CON is a control group, LPC is a live bacterial group, DPC is a dead bacterial group, and BCAA is a branched chain amino acid group);

FIG. 2 shows a graph comparing the relative expression levels of the DNA of Propionibacterium and the blood branched-chain amino acid content of mice in each group after intervention in different groups (wherein FIG. 2a shows the relative content of Primago DNA in feces after intervention in log of 10 copies/ul; FIG. 2b shows the blood branched-chain amino acid level of mice after intervention in each group in umol/L; comparison between groups uses Wilcoxon rank sum test (.times., p < 0.05));

fig. 3 is a graph of results of behavioral tests after intervention of a mouse flora (wherein fig. 3a is a body weight, b is a wire suspension time, c is a number of turns of a rotating rod, d is a rotating rod speed, e is a rotating rod time, f is a cage top suspension time, g is a forelimb grasping force, and h is a hindlimb grasping force);

FIG. 4 shows graphs of relative mRNA expression levels of key proteins in muscle tissue protein synthesis and degradation pathways after intervention of groups of mice (where, graph a is a graph comparing the relative mRNA expression levels of creatine kinase (MCK) in muscle tissue between groups, graph B is a graph comparing the relative mRNA expression levels of MHC-1 (myosin heavy chain 1) in muscle tissue between groups, graph c is a graph comparing the relative mRNA expression levels of MHC-2B (myosin heavy chain 2B) in muscle tissue between groups, graph d is a graph comparing the relative mRNA expression levels of Myogenin (Myogenin) in muscle tissue between groups, graph e is a graph comparing the relative mRNA expression levels of MuRF (muscle specific ring finger protein) in muscle tissue between groups, graph f is a graph comparing the relative mRNA expression levels of Atrogin-1 (muscle atrophy factor) in muscle tissue between groups, CON is a control group, DPC is a live bacterial group, BCA is a branched chain amino acid group.

Detailed Description

EXAMPLE 1 Prevotella copr of the present inventioniAnimal experiments for treating senile debilitating syndrome

Culture and preparation of Propionibacterium 1

Proteus sp（Prevotella copri） Purchased from DSM (18205), the bacteria were routinely streaked onto blood agar TSB dishes after recovery and incubated in an anaerobic tank (80% N2, 10% H2, 10% CO2, 37 ℃). Mature single colonies grown for 3 days, grown on platelets, were then picked under strictly anaerobic conditions and grown overnight in anaerobically sterilized PYG broth containing heme chloride and vitamin K1. Then the anaerobic box is taken out and put inAbout 24 hours on a shaker at 37 ℃. Bacterial density was calculated from OD600 absorbance, bacterial density of 1 OD was approximately 1X 10 ⁹ CFU/ml。

2 raising and grouping of mice

Female C57BL/6 mice (purchased from Beijing vitamin C Lihua) 20 months old were fed in groups of 5 per cage (filter top cage) and were free to eat and drink under a strict 12-hour light cycle. They were acclimatized to the new environment before they were fed with standard food and then divided into four groups, which were filled with sterile PBS, BCAA, live Propionibacterium and heat-inactivated Propionibacterium, at a dose of 5X 10 ⁸ CFUs/100 μl PBS 3 times per week for 6 weeks. Body weight, grip, stick time, etc. were measured before lavage and at the end of the experiment. Fecal samples were collected before lavage and at the end of the experiment and stored at-80 ℃ immediately prior to further analysis. The study did not employ randomization and the acclimated mice were grouped according to body weight to ensure the starting point was the same. All procedures were approved by the animal care committee of the national western medicine institute, university of Sichuan.

3 mouse suspension experiment

Mice were trained prior to the first test. For this, animals were allowed to run three trials, 30 seconds each, with mice resting for 30 minutes between trials. Before starting the test, the mice were placed on the grid, allowed to grasp firmly, and then the grid was turned up quickly. The grid can be placed on a fixing device or can be fixed at a fixed distance (80 cm) from the floor by hand, and a soft cushion is arranged below the grid so as to avoid injury of the mice after falling. A timer was started to record the delay time (in seconds) for the mouse to drop when reversing the grid. The test was repeated 3 times until each mouse completed 3 times, and the maximum value was selected as the final result.

4 mouse grip strength detection experiment

A mouse was removed from the cage and the bottom of the tail was gripped with the thumb and index finger. Placing a grabbing force instrument (Jiangsu Cyons Biotechnology, SA 417) on a horizontal platform, suspending the tail of a mouse, placing the front claw of the mouse on an induction cross rod of the grabbing force instrument, horizontally dragging the tail of the mouse backwards after the front claw of the mouse grabs the cross rod until the front limb of the mouse is loosened, automatically recording the maximum grabbing force by the grabbing force instrument, repeatedly measuring for 3 times, and taking the maximum value to be recorded as the absolute grabbing force of the front limb of the mouse. 1. The minute interval was repeated 3 times and the maximum was selected.

5 mouse rotating rod test experiment

A bar fatigue tester (Jiangsu Saion Biotechnology Co., SA 102) was used in our study. Rotation was started at 4r.p.m. and each mouse was quickly placed in a separate channel of the rotating stick. Once all mice were placed and facing away from the investigator, the rotating rod was accelerated to 40r.p.m. in 300 seconds. The delay time and speed of the mice when dropped were recorded, three experiments were repeated and the maximum was selected.

6. Tissue collection in mice

At the end of the study, a pentobarbital solution with the concentration of 1% is prepared, the mice are injected into the abdominal cavity according to the dosage of 50mg/kg, after the mice are fully anesthetized, the mice are fixed in a supine position, after skin is sterilized, ribs and chest cavities are cut off layer by layer, pericardium is opened, a right hand injector is used, a needle is inserted from the apex of the left ventricle, and 2ml of blood is collected. Blood samples were centrifuged at 3,000 rpm for 15 minutes at 4 ℃. Plasma was isolated and stored at-80 ℃ for subsequent biochemical testing. Then, a perfusion needle is inserted into the heart, frozen heparin physiological saline is slowly and uniformly perfused, meanwhile, the right auricle is cut off, venous blood is released, after the outflow liquid is clear, complete gastrocnemius muscles on two sides of a mouse are rapidly separated on ice, an analytical balance is used for weighing and recording, half of the gastrocnemius muscles are wrapped by tinfoil and then are placed at-80 ℃ for storage for subsequent western-blot experiments, half of the gastrocnemius muscles are soaked in 4% paraformaldehyde solution, and paraffin embedding and slicing are carried out after 24 hours.

7. Mouse blood BCAA assay

The purpose of detecting branched-chain amino acids BCAAs is to find mechanisms by which prevotella copri is involved in improving debilitating syndrome, presumably by promoting metabolism of food by a component or enzyme of prevotella copri and promoting absorption of branched-chain amino acids, while increasing branched-chain amino acid levels in the blood.

Blood was drawn for BCAA measurements before lavage and at the end of the experiment. Blood was collected in EDTA-coated tubes on ice and centrifuged at 1,000g for 10min at 4 ℃ and then stored at-80 ℃ until further analysis. The BCAA assay followed the branched chain amino acid detection kit (ab 83374).

mRNA content determination of 8 mouse muscle tissue related proteins

Total RNA was extracted from muscle tissue using TRIzol reagent. For SYBR Green-based quantitative reverse transcription PCR (qRT-PCR), the isolated RNA was reverse transcribed using Superscript II and random primers (Invitrogen). qRT-PCR analysis was performed in triplicate, data normalized to Rpl27 and Rpl13, and analyzed using qBase software.

9. DNA extraction and quantitative analysis of Propionibacterium in mouse feces

Fecal samples from each mouse were used for metagenomic DNA extraction using QIAamp rapid DNA extraction kit (Qiagen). The method comprises the following specific steps:

(1) Weighing 0.1-0.5g of fecal sample, fully grinding into fine powder in liquid nitrogen, adding 450ul of solution A, shaking and mixing uniformly.

(2) Adding 50ul of solution B, mixing thoroughly, stirring thoroughly, and mixing thoroughly every 2min in water bath at 65deg.C for 6 min.

(3) 100ul of solution C was added and mixed well (without vigorous shaking) and centrifuged at 12000rpm for 10 min.

(4) The supernatant was transferred to a fresh centrifuge tube and centrifuged at 12000rpm for 2 min.

(5) 200ul of solution D is added into an adsorption column, the supernatant after centrifugation is added into the adsorption column with the solution D, and the mixture is blown and sucked by a pipette for several times and uniformly mixed, and is centrifuged at 12000rpm for 1min.

(6) The filtrate in the collection tube was mixed and then re-sucked into the column (as necessary), and centrifuged at 12000rpm for 1min.

(7) The waste liquid in the collection tube was poured out, and 500ul of the rinse liquid was added to the adsorption column, followed by centrifugation at 12000rpm for 1min.

(8) The waste liquid in the collection tube was poured off, and step 7 was repeated twice (three times of rinsing).

(9) The waste liquid in the collection tube was discarded, and the adsorption column was returned to the collection tube and centrifuged at 12000rpm for 2 min.

(10) Taking out the adsorption column, and drying at room temperature for several minutes (due to different seasons and climates), or drying at 50deg.C for 1min.

(11) The column was placed in a new centrifuge tube, 50-100ul of eluent (preheated at 65 ℃) was added, and centrifuged at 12000rpm for 1min.

(12) The liquid in the centrifuge tube was added again to the adsorption column and centrifuged at 12000rpm for 1min. The centrifuge tube is the fecal microorganism DNA solution.

DNA concentration was measured using a NanoDrop 2000 spectrophotometer (Thermo Scientific). The relative DNA content of P.pratensis in mouse faeces was analysed using an MJ OPTICON 2 quantitative PCR apparatus (Bio-Rad, USA) and MJ OPTICON MONITOR analysis software (version 3.1).

10. Statistical analysis of data

All data are expressed as mean ± standard deviation (x±s), a database is built using SPSS 13.0 statistical software, and experimental results are statistically processed using graphpad prism 8.0.1.

11 results of the study

11.1 results of baseline behavioural detection in mice of each group

We monitored the baseline behaviours of each group of mice, including body weight, grip, stick rotation time and suspension time, and analyzed the baseline behaviours of mice found no significant differences between groups as shown in figure 1.

11.2 comparison of the DNA content of the mouse fecal Propionibacterium in groups with the level of branched-chain amino acids in blood

After different groups of mice are respectively infused with gastric physiological saline (control group), live bacteria (Proteus), dead bacteria (Proteus) and Branched Chain Amino Acid (BCAA), the feces of the baseline mice and the feces of the mice after 6 weeks of intervention are collected, DNA quantitative analysis of the Proteus is carried out, the content of the Prussian DNA of the live bacteria group is found to be obviously higher than that of the control group, and the content of the Prussian DNA of the live bacteria group is obviously higher than that of the Prussian DNA before the intervention after the intervention, which indicates that the Prussian is successfully colonized in the intestinal tract of the mice after the Prussian gastric lavage, as shown in figure 2a. The blood branched chain amino acid concentration of the mice in the live bacteria group and the BCAA group after intervention is obviously higher than that of the mice in the control group, the immunity can be improved by improving the quantity of the BCAA, the problems of the immune function reduction and the like caused by the debilitating syndrome can be solved, the synthesis of muscles can be promoted, and the blood branched chain amino acid concentration of the dead bacteria group is not obviously different from that of the mice in the control group, as shown in figure 2b.

11.3 results of behavioral tests after group-independent mouse flora intervention

By detecting the behavioral indexes of the dry prognosis of each group of mice, including weight, holding power, suspension time and rod rotating time, statistical analysis shows that the weight of the mice in the live bacteria group is obviously higher than that of the mice in the control group after intervention, the weights of the mice in the dead bacteria group and the BCAA group are not obviously different from that of the mice in the control group, the holding power of the mice in the live bacteria group after intervention is obviously higher than that of the mice in the control group, the rod rotating time of the mice in the live bacteria group is obviously higher than that of the mice in the control group after intervention, the weights of the mice in the dead bacteria group and the BCAA group are not obviously different from that of the mice in the control group, and the suspension time is not obviously different from that of the mice in the control group, as shown in fig. 3.

Conditions that can improve debilitating syndrome in aged mice after gavage of live bacteria of the genus prunus include iron wire suspension time, stick turning speed, and gripping force of the forelimbs and extremities.

11.4 protein-related RT-PCR detection of muscle tissue after group-wise mouse flora intervention

Relative quantitative analysis of mRNA of related proteins including muscle synthesis related proteins MCK (creatine kinase), MHC-1 (myosin heavy chain 1), MHC-2B (myosin heavy chain 2B), myogenin (Myogenin), and key proteins MuRF (muscle specific ring finger protein) and Atrogin-1 (amyotrophic factor) in the muscle breakdown pathway of the viable bacteria group was performed on muscle tissue (gastrocnemius muscle), and it was found by analysis that the relative mRNA content of the key proteins MuRF and Atrogin-1 in the muscle breakdown pathway of the viable bacteria group was significantly lower than that of the control group, suggesting that the muscle breakdown of the viable bacteria group was reduced as compared with the control group. Whereas the relative mRNA content of Myogenin was significantly reduced in the dead bacterial group only compared to the control group, suggesting that muscle synthesis was reduced in the dead bacterial group compared to the control group, as shown in fig. 4.

In summary, by additionally supplementing mice with live Propionibacterium (prevotella copri), debilitating syndrome can be ameliorated by increasing the motor capacity of the mice, including extending iron wire suspension time, rod rotation time, increasing forelimb and limb grip, increasing the blood branched chain amino acid level of the mice, and enhancing the immunocompetence of the mice.

Claims

Use of 1.Prevotella copri in the manufacture of a medicament for the treatment of senile debilitating syndrome.
2. Use according to claim 1, characterized in that: the Prevotella copri exists as a living bacterium.
3. Use according to claim 1 or 2, characterized in that: the medicine is a preparation prepared by taking Prevotella copri as an active ingredient and adding pharmaceutically acceptable auxiliary materials or auxiliary ingredients.
4. Use according to claim 3, characterized in that: the preparation is an oral preparation.
5. Use according to claim 4, characterized in that: the oral preparation is tablets, capsules, pills and granules.