CN117730912A - Lactobacillus middle-aged and elderly goat milk powder and preparation method thereof - Google Patents
Lactobacillus middle-aged and elderly goat milk powder and preparation method thereof Download PDFInfo
- Publication number
- CN117730912A CN117730912A CN202211151643.7A CN202211151643A CN117730912A CN 117730912 A CN117730912 A CN 117730912A CN 202211151643 A CN202211151643 A CN 202211151643A CN 117730912 A CN117730912 A CN 117730912A
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- goat milk
- parts
- lactobacillus
- milk powder
- aged
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- Dairy Products (AREA)
Abstract
The application discloses lactobacillus middle-aged and elderly goat milk powder, compared with the prior art, the lactobacillus middle-aged and elderly goat milk powder comprises the following components in parts by weight: 150-570 parts of whole goat milk powder, 10-120 parts of trehalose, 16-145 parts of inulin, 0.01-7 parts of protease, 0.01-9 parts of lactobacillus powder and 0.01-0.6 part of compound vitamin. The lactobacillus middle-aged and elderly goat milk powder provided by the application has strong thallus stability and good heat resistance, and reduces the loss of nutrient components of the lactobacillus middle-aged and elderly goat milk powder after hot water brewing.
Description
Technical Field
The application relates to the field of goat milk powder and a preparation method thereof, in particular to lactobacillus middle-aged and elderly goat milk powder and a preparation method thereof.
Background
Nowadays, health care consciousness of people is gradually enhanced, the nutritional value of milk products is accepted by more people, whether the milk products are adult men or women or middle-aged and elderly people, people are enthusiastically taking the milk products for nourishing, and especially, the milk products are rich in calcium, are more beneficial to the middle-aged and elderly people to strengthen physique and improve body resistance, are nutritional supplements for the middle-aged and elderly people, and the goat milk is a food with extremely high nutritional value, plays a vital role in human nutrition, contains abundant proteins, fatty acids and vitamins, and therefore has great biological value, compared with human milk and milk, the goat milk contains higher levels of calcium, phosphorus and potassium, and has relatively higher protein content, small fat globules and protein particles, uniform particle size, thin and soft protein clots and short fat chain, is more beneficial to direct absorption and utilization by human bodies, and the human body has higher and higher acceptance of the goat milk.
The functional dairy product is dairy products with different physiological activities by adding probiotics or physiological active substances, and along with the development of the functional dairy product, consumers pay more attention to maintaining or improving health through the functional dairy product, and the existing lactobacillus milk powder for middle-aged and elderly people is low in survival rate of thalli after hot water brewing due to poor heat resistance of thalli, so that the nutrition function of the milk powder is influenced.
Therefore, how to improve the heat resistance of the thalli in the lactobacillus milk powder for middle-aged and elderly people and reduce the loss of the nutrition components of the lactobacillus milk powder for middle-aged and elderly people after hot water brewing is a problem to be solved by the technicians in the field.
Disclosure of Invention
In order to solve the technical problems, the application provides the lactobacillus middle-aged and elderly goat milk powder and the preparation method thereof, which can improve the heat resistance of thalli in the lactobacillus middle-aged and elderly goat milk powder and reduce the loss of nutrient components of the lactobacillus middle-aged and elderly goat milk powder after hot water brewing.
The technical scheme provided by the application is as follows:
the application provides lactobacillus middle-aged and elderly goat milk powder, which comprises the following components in parts by weight: 150-570 parts of whole goat milk powder, 10-120 parts of trehalose, 16-145 parts of inulin, 0.01-7 parts of protease, 0.01-9 parts of lactobacillus powder and 0.01-0.6 part of compound vitamin.
Further, in a preferred mode of the present application, the composition comprises the following components by weight: 220-570 parts of whole goat milk powder, 28-110 parts of trehalose, 21-130 parts of inulin, 0.05-6 parts of protease, 0.02-8.7 parts of lactobacillus powder and 0.05-0.6 part of compound vitamin.
Further, in a preferred mode of the present application, the protease is Alcalase alkaline protease.
Further, in a preferred mode of the present application, the lactobacillus powder comprises: lactobacillus plantarum powder and lactobacillus rhamnosus powder, wherein the mass ratio of the lactobacillus plantarum powder to the lactobacillus rhamnosus powder is 1:1-5.
Compared with the prior art, the lactobacillus middle-aged and elderly goat milk powder comprises the following components in parts by weight: 220-570 parts of whole milk goat milk powder, 28-110 parts of trehalose, 21-130 parts of inulin, 0.05-6 parts of Alcalase enzyme, 0.02-8.7 parts of lactobacillus powder and 0.05-0.6 part of compound vitamin, compared with the prior art, the lactobacillus middle-aged and elderly goat milk powder disclosed by the application can improve the heat resistance of lactobacillus and reduce the loss of nutritional ingredients of the lactobacillus middle-aged and elderly goat milk powder after hot water brewing through the matching of the components and the proportions of the components.
The application also provides a preparation method of the lactobacillus middle-aged and elderly goat milk powder, which comprises the following steps: s1, mixing 220-570 parts of full-fat goat milk powder with 0.05-0.6 part of compound vitamin, and adding water to prepare recovered goat milk with the concentration of 8% -23%; s2, pasteurizing the reconstituted goat milk, and after the reconstituted goat milk is completely cooled, performing pasteurization according to the ratio of 17-32:1: adding activated lactobacillus plantarum and activated lactobacillus rhamnosus according to the mass ratio of 1-5 to obtain first goat milk; s3, dissolving 0.01-7 parts of Alcalase enzyme in sterile water, sterilizing, adding the sterilized Alcalase enzyme into the first goat milk, shaking uniformly, and culturing at constant temperature for 5-28 hours to obtain second goat milk; s4, mixing 220-570 parts of whole goat milk powder, 28-110 parts of trehalose and 21-130 parts of inulin to obtain a composite heat-resistant protective agent; and S5, adding the composite heat-resistant protective agent into the second goat milk, uniformly mixing, and performing spray drying to obtain lactobacillus middle-aged and elderly goat milk powder.
Further, in a preferred mode of the present application, the concentration of the reconstituted goat milk in S1 is 10% to 14%.
Further, in a preferred mode of the present application, the pasteurization in S2 takes place for a period of 10-35min at a temperature of 90 ℃.
Further, in a preferred mode of the present application, the sterilization mode in S3 is sterilization using a filtration membrane of 0.22 μm.
Further, in a preferred mode of the present application, the mass ratio of the components of the composite heat-resistant protective agent in S4 is: full-fat sheep milk powder: trehalose: inulin=7 to 13:1:1.
Further, in a preferred mode of the present application, the spray-drying in S5 has an inlet temperature of 100 to 150 ℃ and an outlet temperature of 60 to 90 ℃.
Compared with the prior art, the technical scheme provided by the application has the advantages that the lactobacillus middle-aged and elderly goat milk powder prepared by the preparation method of the lactobacillus middle-aged and elderly goat milk powder can improve the heat resistance of lactobacillus, and the loss of nutrient components of the lactobacillus middle-aged and elderly goat milk powder after hot water brewing is reduced.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic step diagram of a preparation method of lactobacillus middle-aged and elderly goat milk powder provided in an embodiment of the application.
Detailed Description
In order to better understand the technical solutions in the present application, the following description will clearly and completely describe the technical solutions in the embodiments of the present application with reference to the drawings in the embodiments of the application, and it is obvious that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by one of ordinary skill in the art without undue burden from the present disclosure, are within the scope of the present disclosure.
It will be understood that when an element is referred to as being "fixed" or "disposed" on another element, it can be directly on the other element or be indirectly on the other element; when an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.
It is to be understood that the terms "length," "width," "upper," "lower," "front," "rear," "first," "second," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate an orientation or positional relationship based on that shown in the drawings, merely for convenience of description and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus are not to be construed as limiting the present application.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" or "a number" is two or more, unless explicitly defined otherwise.
It should be understood that the structures, proportions, sizes, etc. shown in the drawings are merely illustrative of the disclosure, and should not be construed as limiting the application to the understanding and appreciation of the technical scope of the present disclosure, any structural modifications, changes in proportions, or adjustments of sizes, which do not affect the efficiency or otherwise, the purpose of the present disclosure, are included within the spirit and scope of the present disclosure.
Compared with the prior art, the lactobacillus middle-aged and elderly goat milk powder provided by the application comprises the following components in parts by weight: 150-570 parts of whole goat milk powder, 10-120 parts of trehalose, 16-145 parts of inulin, 0.01-7 parts of protease, 0.01-9 parts of lactobacillus powder and 0.01-0.6 part of compound vitamin, so that the heat resistance of lactobacillus can be improved and the loss of nutrient components of lactobacillus middle-aged and elderly milk powder after hot water brewing can be reduced through the matching of the components and the proportion of the components.
Specifically, in the embodiment of the application, the composition comprises the following components in parts by weight: 220-570 parts of whole goat milk powder, 28-110 parts of trehalose, 21-130 parts of inulin, 0.05-6 parts of protease, 0.02-8.7 parts of lactobacillus powder and 0.05-0.6 part of compound vitamin.
More specifically, the vitamin complex comprises vitamins, minerals and trace elements.
Still further, in an embodiment of the present application, the multivitamin comprises: vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D1, vitamin E, biotin, calcium, phosphorus, copper and manganese.
It should be noted that, in the embodiment of the present application, the lactobacillus middle-aged and elderly goat milk powder further includes: 0.1 to 2.8 parts by weight of choline.
More specifically, the choline is 0.2-1.3 parts.
Still further, in an embodiment of the present application, the method further includes: ferrous sulfate 0.05-4.7 weight portions.
More specifically, the ferrous sulfate is 0.05-0.9 parts.
Still further, in an embodiment of the present application, the method further includes: 0.1-5 parts by weight of zinc sulfate.
More specifically, the zinc sulfate is 0.1 to 1.1 parts.
Specifically, in the embodiments of the present application, the protease is Alcalase alkaline protease.
Specifically, in the embodiment of the present application, the lactobacillus powder includes: lactobacillus plantarum powder and lactobacillus rhamnosus powder, wherein the mass ratio of the lactobacillus plantarum powder to the lactobacillus rhamnosus powder is 1:1-5.
More specifically, the mass ratio of the lactobacillus plantarum powder to the lactobacillus rhamnosus powder is 1:1.
Furthermore, in the embodiment of the application, the following components of the lactobacillus middle-aged and elderly goat milk powder have the following effects:
trehalose: trehalose is a non-reducing natural saccharide with high safety, has biological functions of moisture retention, cold resistance, drought resistance, heat resistance, acid resistance and the like, and has non-specific protection effect on common biological macromolecules.
Inulin: inulin is a fructose polymer, linked by (2-1) glycosidic linkages, which can have a degree of polymerization of up to 60 units, and which can be hydrolyzed by inulase to fructooligosaccharides, inulin and fructooligosaccharides also commonly being used as encapsulants for certain probiotic strains in combination with other materials.
Compound vitamins: can effectively strengthen physique, promote blood circulation, help muscle growth and supplement microelements.
Lactobacillus plantarum: the lactobacillus plantarum can improve and regulate the balance of intestinal microbial flora, enhance the immunity of organisms, inhibit pathogenic bacteria and regulate the microecological balance of intestinal tracts by competing with pathogenic bacteria to nutrients through generating various antibacterial substances such as organic acid, bacteriocin, hydrogen peroxide, diacetyl and the like, so as to form a biological barrier, and meanwhile, the metabolites of the lactobacillus plantarum, namely the lactobacillus and the bacteriocin, have the effects of inhibiting the proliferation of other harmful bacteria in the intestinal tracts, maintain and ensure the optimal dominant combination of the microbial flora and the stability of the combination, prevent the colonization and invasion of pathogenic bacteria, inhibit the growth of pathogenic bacteria and harmful microorganisms and the adhesion of toxins.
Rhamnella: the lactobacillus rhamnosus has the functions of regulating intestinal flora, promoting the growth and playing roles of bifidobacteria, is favorable for stopping diarrhea, promoting toxin discharge of organisms, enhancing organism resistance, regulating allergic constitution, and achieving the effect of preventing cold by improving immunity, and has the characteristics of loose requirements on production environment and good acid resistance and oxygen resistance compared with other probiotics.
Choline: the choline is one of lipophilic vitamin B groups, can enter brain cells to generate substances capable of helping memory, so that the choline has the effects of promoting the development of brain cells and brain nervous systems, improving thinking and memory, is also called a memory factor, is an important raw material for synthesizing acetylcholine, can help central nerves to transfer information, is a necessary substance for mental activities such as brain thinking and memory, can also improve the ability of liver to utilize fatty acid, thereby preventing excessive accumulation of fat in the liver, has the effect of promoting liver functions, can help human tissues to remove toxins and medicines, can emulsify cholesterol, and can avoid accumulation of cholesterol in artery walls or gall bladder.
Ferrous sulfate: the method is mainly used for correcting iron deficiency anemia, iron is a component element of hemoglobin in red blood cells, and the amount of the hemoglobin synthesized by the red blood cells is reduced when iron deficiency occurs, so that the volume of the red blood cells is reduced, the oxygen carrying capacity is reduced, and iron deficiency anemia is formed.
Zinc sulfate: zinc sulfate is a zinc supplementing medicine, and can relieve anorexia, anemia, etc., and can be used for adjuvant treatment of rheumatoid arthritis, acne, chronic ulcer, colitis, aphtha, etc.
The application also provides a preparation method of the lactobacillus middle-aged and elderly goat milk powder, which comprises the following steps: s1, mixing 220-570 parts of full-fat goat milk powder, 0.05-0.9 part of ferrous sulfate, 0.05-0.6 part of compound vitamin, 0.2-1.3 parts of choline and 0.1-1.1 parts of zinc sulfate, and adding water to prepare recovered goat milk with the concentration of 8-23%; s2, pasteurizing the reconstituted goat milk, and after the reconstituted goat milk is completely cooled, performing pasteurization according to the ratio of 17-32:1: 1, adding activated lactobacillus plantarum and activated lactobacillus rhamnosus to obtain first goat milk; s3, dissolving 0.01-7 parts of Alcalase enzyme in sterile water, sterilizing, adding the sterilized Alcalase enzyme into the first goat milk, shaking uniformly, and culturing at constant temperature for 5-28 hours to obtain second goat milk; s4, mixing 220-570 parts of whole goat milk powder, 28-110 parts of trehalose and 21-130 parts of inulin to obtain a composite heat-resistant protective agent; and S5, adding the composite heat-resistant protective agent into the second goat milk, uniformly mixing, and performing spray drying to obtain lactobacillus middle-aged and elderly goat milk powder.
The lactobacillus plantarum activation step in the step S2 is to be supplemented with: placing the freeze-dried powder of lactobacillus plantarum in a first MRS culture medium sterilized at 120 ℃, culturing at a constant temperature of 37 ℃ in an incubator for 12 hours, centrifuging to obtain a first lactobacillus plantarum suspension, inoculating the first lactobacillus plantarum suspension into a second MRS culture medium according to an inoculum size of 5%, culturing at a constant temperature of 37 ℃ in the incubator for 12 hours, centrifuging to obtain a second lactobacillus plantarum suspension, inoculating the second lactobacillus plantarum suspension into a third MRS culture medium according to an inoculum size of 5%, culturing at a constant temperature of 37 ℃ in the incubator for 12 hours, centrifuging to obtain a third lactobacillus plantarum suspension, and activating lactobacillus plantarum.
More specifically, the first MRS medium, the second MRS medium, and the third MRS medium are the same medium.
More specifically, the lactobacillus rhamnosus activation step in step S2 is the same as the lactobacillus plantarum activation step in step S2, i.e. the lactobacillus rhamnosus activation step in step S2: placing the freeze-dried powder of lactobacillus rhamnosus in a first MRS culture medium sterilized at 120 ℃, culturing at a constant temperature of 37 ℃ for 12 hours in an incubator, centrifuging to obtain a first lactobacillus rhamnosus suspension, inoculating the first lactobacillus rhamnosus suspension into a second MRS culture medium according to an inoculum size of 5%, culturing at a constant temperature of 37 ℃ for 12 hours in the incubator, centrifuging to obtain a second lactobacillus rhamnosus suspension, inoculating the second lactobacillus rhamnosus suspension into a third MRS culture medium according to an inoculum size of 5%, culturing at a constant temperature of 37 ℃ for 12 hours in the incubator, centrifuging to obtain a third lactobacillus rhamnosus suspension, and completing the activation of lactobacillus rhamnosus.
More specifically, the S2 is: pasteurizing the reconstituted goat milk, and after complete cooling, according to the ratio of 17-32:1: and adding the third lactobacillus plantarum suspension and the third lactobacillus rhamnosus suspension according to the mass ratio of 1 to obtain the first goat milk.
Further, in the embodiment of the present application, the mixing mass ratio of the reconstituted goat milk to the third lactobacillus plantarum suspension and the third lactobacillus rhamnosus suspension in the S2 is 28:1:1.
It should be noted that, in the embodiment of the present application, the compound heat-resistant protective agent in S4 comprises the following components in proportion: 240 parts of full-fat goat milk powder, 29 parts of trehalose and 25 parts of inulin.
Specifically, in the embodiment of the present application, the concentration of the reconstituted goat milk in S1 is 10% -14%.
More specifically, the concentration of reconstituted goat milk in S1 is 12%.
Specifically, in the embodiment of the present application, the pasteurization in S2 is performed for 10-35min at 90 ℃.
More specifically, the pasteurization in S2 takes 15 minutes.
Specifically, in the embodiment of the present application, the sterilization mode in S3 is to use a filtration membrane with a size of 0.22 μm for sterilization.
More specifically, the time of the constant temperature culture in S3 is 12 hours, and the temperature is 37 ℃.
Specifically, in the embodiment of the application, the mass ratio of the components of the composite heat-resistant protective agent in S4 is: full-fat sheep milk powder: trehalose: inulin=7 to 13:1:1.
Specifically, in the embodiment of the present application, the inlet temperature of the spray drying in S5 is 100-150 ℃ and the outlet temperature is 60-90 ℃.
More specifically, the spray-drying in S5 has an inlet temperature of 130 ℃ and an outlet temperature of 70 ℃.
More specifically, the lactobacillus middle-aged and elderly goat milk powder comprises the following components in parts by weight: 550 parts of full-fat goat milk powder, 45 parts of trehalose, 55 parts of inulin, 2.3 parts of protease, 6.1 parts of lactobacillus powder, 0.14 part of compound vitamin, 0.07 part of ferrous sulfate, 0.9 part of choline and 0.3 part of zinc sulfate.
In order to better illustrate the influence of the composite heat-resistant protective agent of the lactobacillus middle-aged and elderly goat milk powder related to the embodiment of the application on the survival rate of thalli, the preparation of the lactobacillus middle-aged and elderly goat milk powder is illustrated by preparing the heat-resistant protective agent with different weight proportion and composition components:
example 1:
the embodiment provides lactobacillus middle-aged and elderly goat milk powder, which comprises the following total components: 220-570 parts of full-fat goat milk powder, 2.3 parts of protease, 6.1 parts of lactobacillus powder, 0.14 part of compound vitamin, 0.07 part of ferrous sulfate, 0.9 part of choline and 0.3 part of zinc sulfate.
Mixing 550 parts of full-fat goat milk powder, 0.07 part of ferrous sulfate, 0.14 part of compound vitamin, 0.07 part of ferrous sulfate, 0.9 part of choline and 0.3 part of zinc sulfate, adding water to prepare 12% strength reconstituted goat milk, pasteurizing the reconstituted goat milk, and cooling completely according to the following ratio of 28:1: adding a third lactobacillus plantarum suspension and a third lactobacillus rhamnosus suspension according to the mass ratio of 1 to obtain first goat milk, dissolving 2.3 parts of Alcalase enzyme in sterile water, adding the sterilized and sterilized first goat milk, shaking uniformly, culturing at constant temperature for 5-28 hours to obtain second goat milk, equally dividing the second goat milk into 6 equal parts, respectively adding 0 part, 2 parts, 3 parts, 4 parts, 5 parts and 6 parts of whole milk powder into 6 parts of the second goat milk, uniformly mixing, maintaining the temperature of 75 ℃ for heating in a water bath for 10 minutes, taking out, rapidly cooling to room temperature, diluting by 3-6 times, and carrying out viable count to obtain a comparison table of the influence of the whole goat milk powder on the survival rate of thalli.
Example 2:
the embodiment provides lactobacillus middle-aged and elderly goat milk powder, which comprises the following components in parts by weight: 220-570 parts of full-fat goat milk powder, 45 parts of trehalose, 2.3 parts of protease, 6.1 parts of lactobacillus powder, 0.14 part of compound vitamin, 0.07 part of ferrous sulfate, 0.9 part of choline and 0.3 part of zinc sulfate.
Mixing 550 parts of full-fat goat milk powder, 0.07 part of ferrous sulfate, 0.14 part of compound vitamin, 0.07 part of ferrous sulfate, 0.9 part of choline and 0.3 part of zinc sulfate, adding water to prepare 12% strength reconstituted goat milk, pasteurizing the reconstituted goat milk, and cooling completely according to the following ratio of 28:1: adding a third lactobacillus plantarum suspension and a third lactobacillus rhamnosus suspension according to the mass ratio of 1 to obtain first goat milk, dissolving 2.3 parts of Alcalase enzyme in sterile water, adding the sterilized and sterilized first goat milk, shaking uniformly, culturing at constant temperature for 5-28 hours to obtain second goat milk, equally dividing the second goat milk into 6 equal parts, respectively adding 0 part, 1 part, 3 parts, 5 parts, 7 parts and 9 parts of trehalose into 6 parts of the second goat milk, uniformly mixing, maintaining the temperature of 75 ℃ for heating in a water bath for 10 minutes, taking out, rapidly cooling to room temperature, diluting by 3-6 times, and carrying out viable count to obtain a comparison table of the influence of the trehalose on the survival rate of thalli as shown in table 2.
Example 3:
the embodiment provides lactobacillus middle-aged and elderly goat milk powder, which comprises the following components in parts by weight: 220-570 parts of full-fat goat milk powder, 55 parts of inulin, 2.3 parts of protease, 6.1 parts of lactobacillus powder, 0.14 part of compound vitamin, 0.07 part of ferrous sulfate, 0.9 part of choline and 0.3 part of zinc sulfate.
Mixing 550 parts of full-fat goat milk powder, 0.07 part of ferrous sulfate, 0.14 part of compound vitamin, 0.07 part of ferrous sulfate, 0.9 part of choline and 0.3 part of zinc sulfate, adding water to prepare 12% strength reconstituted goat milk, pasteurizing the reconstituted goat milk, and cooling completely according to the following ratio of 28:1: adding a third lactobacillus plantarum suspension and a third lactobacillus rhamnosus suspension according to the mass ratio of 1 to obtain first goat milk, dissolving 2.3 parts of Alcalase enzyme in sterile water, adding the sterilized and sterilized first goat milk, shaking uniformly, culturing at constant temperature for 5-28 hours to obtain second goat milk, equally dividing the second goat milk into 6 equal parts, respectively adding 0 part, 1 part, 3 parts, 5 parts, 7 parts and 9 parts of inulin into 6 parts of second goat milk, uniformly mixing, heating in a water bath at the temperature of 75 ℃ for 10 minutes, taking out, rapidly cooling to room temperature, diluting by 3-6 times, and performing viable count to obtain an influence comparison table of inulin as shown in table 3 on the survival rate of thalli.
Example 4:
the embodiment provides lactobacillus middle-aged and elderly goat milk powder, which comprises the following components in parts by weight: 220-570 parts of full-fat goat milk powder, 45 parts of trehalose, 55 parts of inulin, 2.3 parts of protease, 6.1 parts of lactobacillus powder, 0.14 part of compound vitamin, 0.07 part of ferrous sulfate, 0.9 part of choline and 0.3 part of zinc sulfate.
Mixing 550 parts of full-fat goat milk powder, 0.07 part of ferrous sulfate, 0.14 part of compound vitamin, 0.07 part of ferrous sulfate, 0.9 part of choline and 0.3 part of zinc sulfate, adding water to prepare 12% strength reconstituted goat milk, pasteurizing the reconstituted goat milk, and cooling completely according to the following ratio of 28:1: adding a third lactobacillus plantarum suspension and a third lactobacillus rhamnosus suspension according to the mass ratio of 1 to obtain first goat milk, dissolving 2.3 parts of Alcalase enzyme in sterile water, adding the sterilized and sterilized first goat milk, shaking uniformly, culturing at constant temperature for 5-28 hours to obtain second goat milk, mixing 240 parts of whole-fat goat milk powder, 29 parts of trehalose and 25 parts of inulin to obtain a composite heat-resistant protective agent, equally dividing the second goat milk into 6 parts, respectively adding 0 part, 0.5 part, 1 part, 1.5 part, 2 parts and 2.5 parts of the composite heat-resistant protective agent into 6 parts of the second goat milk, uniformly mixing, heating in a water bath at the temperature of 75 ℃ for 10 minutes, taking out, rapidly cooling to room temperature, diluting by 3-6 times, and carrying out viable count to obtain a comparative table of the influence of the composite heat-resistant protective agent on the survival rate of thalli as shown in table 4.
TABLE 1 comparative Table of the effects of full fat sheep milk powder on the survival rate of cells
| Full fat sheep milk powder adding amount (g/L) | Number of viable bacteria (10) 5 cfu/mL) | Survival (%) |
| 0 | 3.6 | 1.36 |
| 20 | 10.7 | 4.04 |
| 30 | 13.4 | 5.06 |
| 40 | 14.5 | 5.48 |
| 50 | 19.2 | 7.25 |
| 60 | 12.2 | 4.61 |
Wherein, 1 part of full-fat goat milk powder is 10g.
TABLE 2 comparison of the Effect of trehalose on cell viability
Wherein 1 part of trehalose is 1g.
TABLE 3 comparison of the influence of inulin on the viability of cells
| Inulin addition (g/L) | Number of viable bacteria (10) 5 cfu/mL) | Survival (%) |
| 0 | 4.01 | 1.37 |
| 2 | 7.16 | 2.44 |
| 3 | 8.74 | 2.99 |
| 4 | 9.05 | 3.09 |
| 5 | 10.15 | 3.47 |
| 6 | 9.37 | 3.20 |
Wherein 1 part of inulin is 1g.
TABLE 4 comparative Table of the effect of the Compound Heat-resistant protectant on the cell viability
| The addition amount (g/L) of the composite heat-resistant protective agent | Number of viable bacteria (10) 5 cfu/mL) | Survival (%) |
| 0 | 4.03 | 1.37 |
| 2 | 88.69 | 30.15 |
| 10 | 68.89 | 23.42 |
| 15 | 57.77 | 19.64 |
| 20 | 46.27 | 15.73 |
| 25 | 27.97 | 9.51 |
Wherein, 1 part of the composite heat-resistant protective agent is 10g.
Since it is difficult to ensure that the experimental conditions are completely consistent, the experimental data have a certain error but do not affect the final experimental result, and it is clear from Table 1 that the number of viable bacteria and the survival rate of cells per unit volume of the second sheep milk start to rise as the whole milk powder is added, and when the addition amount is 50g/L, the number of viable bacteria and the survival rate of cells are maximized, and then the number of viable bacteria and the survival rate of cells gradually decrease, so that the heat resistance of cells can be enhanced after a certain amount of whole milk powder is added to the second sheep milk, the survival rate of cells in the unit volume of the second sheep milk can be increased, and from Table 2, the number of viable bacteria and the survival rate of cells of the unit volume of the second sheep milk start to increase and then decrease as the addition amount is increased, and when the addition amount is 4g/L, the number of viable bacteria and the survival rate of cells are maximized, when the addition amount was more than 4g/L, the number of viable bacteria and the survival rate of the cells began to decrease, and therefore, when a certain amount of trehalose was added to the second sheep milk, the heat resistance of the cells could be enhanced, and when a certain amount of inulin was added to the second sheep milk, the survival rate of the cells could be enhanced, as shown in Table 3, after the addition of inulin to the second sheep milk, the number of viable bacteria and the survival rate of the cells per unit volume of the second sheep milk tended to increase and then decrease, and when the addition amount was 5g/L, the number of viable bacteria and the survival rate of the cells became maximum, and when the addition amount was more than 4g/L, the number of viable bacteria and the survival rate of the cells began to decrease, and therefore, when a certain amount of inulin was added to the second sheep milk, the heat resistance of the cells could be enhanced, and as shown in tables 1 to 3, the survival rate of the cells per unit volume of the second sheep milk could be enhanced, the three components in the composite heat-resistant protective agent have the following effects on the bacterial survival rate in sequence: full fat sheep milk powder > inulin > trehalose.
As can be seen from table 4, after the compound heat-resistant protective agent is added to the second goat milk, the number of viable bacteria and the survival rate of the second goat milk per unit volume tend to increase and decrease after the addition amount increases, when the addition amount is 20g/L, the number of viable bacteria and the survival rate of the bacteria reach the maximum, and when the addition amount is more than 20g/L, the number of viable bacteria and the survival rate of the bacteria start to decrease, so that after a certain amount of compound heat-resistant protective agent is added to the second goat milk, the heat resistance of the bacteria can be enhanced, the survival rate of the bacteria in the second goat milk per unit volume can be obviously improved, and in 4 examples of the lactobacillus middle-aged and elderly goat milk powder related to the application, the effect on the survival rate of the bacteria is as follows: the composite heat-resistant protective agent is more than full-fat goat milk powder is more than inulin is more than trehalose, so that the lactobacillus middle-aged and elderly goat milk powder comprising the composite heat-resistant protective agent has the highest cell survival rate, and the lactobacillus middle-aged and elderly goat milk powder comprises the following components in parts by weight: 220-570 parts of whole goat milk powder, 45 parts of trehalose, 55 parts of inulin, 2.3 parts of protease, 6.1 parts of lactobacillus powder, 0.14 part of compound vitamin, 0.07 part of ferrous sulfate, 0.9 part of choline and 0.3 part of zinc sulfate, has strong thallus stability, can prevent the thallus from being heated to die in a large amount, is favorable for preserving the nutritional ingredients in lactobacillus middle-aged and elderly goat milk powder, and reduces the loss of the nutritional ingredients of lactobacillus middle-aged and elderly goat milk powder after hot water brewing.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. The lactobacillus middle-aged and elderly goat milk powder is characterized by comprising the following components in parts by weight: 150-570 parts of whole goat milk powder, 10-120 parts of trehalose, 16-145 parts of inulin, 0.01-7 parts of protease, 0.01-9 parts of lactobacillus powder and 0.01-0.6 part of compound vitamin.
2. The lactobacillus middle-aged and elderly goat milk powder according to claim 1, which is characterized by comprising the following components in parts by weight: 220-570 parts of whole goat milk powder, 28-110 parts of trehalose, 21-130 parts of inulin, 0.05-6 parts of protease, 0.02-8.7 parts of lactobacillus powder and 0.05-0.6 part of compound vitamin.
3. The lactic acid bacteria middle-aged and elderly goat milk powder of claim 2, wherein the protease is Alcalase alkaline protease.
4. The lactobacillus middle-aged and elderly goat milk powder according to claim 3, wherein the lactobacillus powder comprises: lactobacillus plantarum powder and lactobacillus rhamnosus powder, wherein the mass ratio of the lactobacillus plantarum powder to the lactobacillus rhamnosus powder is 1:1-5.
5. A method for preparing the lactobacillus middle-aged and elderly goat milk powder according to any one of claims 1 to 4, comprising the following steps:
s1, mixing 220-570 parts of full-fat goat milk powder with 0.05-0.6 part of compound vitamin, and adding water to prepare recovered goat milk with the concentration of 8% -23%;
s2, pasteurizing the reconstituted goat milk, and after the reconstituted goat milk is completely cooled, performing pasteurization according to the ratio of 17-32:1: adding activated lactobacillus plantarum and activated lactobacillus rhamnosus according to the mass ratio of 1-5 to obtain first goat milk;
s3, dissolving 0.01-7 parts of Alcalase enzyme in sterile water, sterilizing, adding the sterilized Alcalase enzyme into the first goat milk, shaking uniformly, and culturing at constant temperature for 5-28 hours to obtain second goat milk;
s4, mixing 220-570 parts of whole goat milk powder, 28-110 parts of trehalose and 21-130 parts of inulin to obtain a composite heat-resistant protective agent;
and S5, adding the composite heat-resistant protective agent into the second goat milk, uniformly mixing, and performing spray drying to obtain lactobacillus middle-aged and elderly goat milk powder.
6. The method for preparing the lactobacillus middle-aged and elderly goat milk powder according to claim 5, wherein the concentration of the reconstituted goat milk in the S1 is 10% -14%.
7. The method for preparing the lactobacillus middle-aged and elderly goat milk powder according to claim 6, wherein the pasteurization time in the step S2 is 10-35min, and the temperature is 90 ℃.
8. The method for preparing the lactobacillus middle-aged and elderly goat milk powder according to claim 7, wherein the sterilization mode in the step S3 is to perform sterilization by adopting a filtering membrane with the diameter of 0.22 μm.
9. The preparation method of the lactobacillus middle-aged and elderly goat milk powder according to claim 8, wherein the mass ratio of the components of the composite heat-resistant protective agent in the S4 is as follows: full-fat sheep milk powder: trehalose: inulin=7 to 13:1:1.
10. The method for preparing the lactobacillus middle-aged and elderly goat milk powder according to claim 5, wherein the inlet temperature of the spray drying in the step S5 is 100-150 ℃ and the outlet temperature is 60-90 ℃.
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