CN1176367C - Automatic focus regulator for laser cofocal scanner - Google Patents
Automatic focus regulator for laser cofocal scanner Download PDFInfo
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- CN1176367C CN1176367C CNB021002622A CN02100262A CN1176367C CN 1176367 C CN1176367 C CN 1176367C CN B021002622 A CNB021002622 A CN B021002622A CN 02100262 A CN02100262 A CN 02100262A CN 1176367 C CN1176367 C CN 1176367C
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- 230000003287 optical effect Effects 0.000 claims abstract description 16
- 230000003321 amplification Effects 0.000 claims description 9
- 238000002955 isolation Methods 0.000 claims description 9
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 9
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 8
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 230000005540 biological transmission Effects 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract 3
- 239000000523 sample Substances 0.000 abstract 3
- 239000012472 biological sample Substances 0.000 abstract 1
- 241000219739 Lens Species 0.000 description 48
- 210000000695 crystalline len Anatomy 0.000 description 48
- 238000003384 imaging method Methods 0.000 description 5
- 238000006073 displacement reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005755 formation reaction Methods 0.000 description 2
- 239000000919 ceramic Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001459 lithography Methods 0.000 description 1
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Abstract
The present invention relates to an automatic focusing device of a laser confocal scanner, which belongs to the field of photoelectric detection devices for biological instruments, medical care instruments and scientific instruments. The present invention is characterized in that the automatic focusing device comprises an optical head, an input optical path, a reflecting optical path for focusing and a reflecting optical path for detection, wherein the input optical path is orderly composed of a laser device, a needle hole, a lens, an objective lens and a sample; the reflecting optical path for focusing is composed of the sample, the objective lens, a beam splitter mirror, an astigmatic lens and a quadrant photoelectric detector; the reflecting optical path for detection is orderly composed of the sample, the objective lens, a dichroic beamsplitter, the lens, the needle hole and a photomultiplier tube; the focusing control circuit is a digital control circuit which is mainly composed of a digital signal processor, a computer and an actuating motor; the focusing mechanism is a screw rod-nut type focusing mechanism connected with the output axis of an actuating circuit, and meanwhile, the automatic focusing device is provided with a photoelectric control type limit switch. The automatic focusing device can detect biological samples in an automatic focusing mode and has the advantages of large focusing range and high precision.
Description
Technical field
The Electro-Optical Sensor Set field that the automatic focusing device of laser cofocal scanner belongs to is biological, medical treatment, scientific instrument are used.
Background technology:
Laser cofocal scanner is compared with simple microscope, has higher resolution, is widely used in biology, life, medical treatment and the scientific instrument.Existing focusing technology and device, they are researched and developed at different technical fields, can not be applicable to laser cofocal scanner fully.As the patent No. is ZL98229444.1, the Chinese utility model patent of " focus control of lithography machine of submicron " by name discloses a kind of litho machine focus control, it is characterized by: the imaging of mark is through being carved object, little object lens, behind the catoptron, be divided into two-way by spectroscope, one road catoptric imaging is in the four-quadrant receiver, another Reuter penetrates through imagery of cylindrical mirror in ccd image sensor, two-way output defocus signal and target amount of bias are sent into single-chip microcomputer, the control piezoelectric ceramics promotes flexible hinge, drive quilt and carve the motion that object is made big displacement or micrometric displacement, reach the target position of focal plane.It does not detect and uses reflected light path, is not suitable in the laser cofocal scanner.
Goal of the invention
The object of the present invention is to provide a kind of automatic focusing device of laser cofocal scanner.
The invention is characterized in: the automatic focusing device of laser cofocal scanner, contain confocal light path and focusing light path, focusing control circuit and focus adjusting mechanism is characterized in that:
Described confocal light path and focusing light path contain successively by laser instrument 1, first pin hole 2, lens 3, two input paths with dichroic beamsplitter (4,5), object lens 6 and sample 8 formations at optical axis angle at 45, wherein laser instrument 1 is near first pin hole 2, first pin hole 2 is positioned on the front focal plane of lens 3, and sample 8 is placed on the back focal plane of object lens 6;
Along what the light path from sample laser light reflected and the fluorescence institute approach that inspires set gradually be: described object lens 6, described dichroic beamsplitter 5, lens 3, pin hole 2 and photomultiplier 10, wherein said photomultiplier 10 and second pin hole 2 are near placement, described second pin hole 2 is positioned on the back focal plane of lens 3, and this road is confocal light path;
Along what another light path from sample laser light reflected and the fluorescence institute approach that inspires set gradually be: described object lens 6, described two dichroic beamsplitters 5,4, astigmatic lens 11 and four-quadrant photo detector 12, wherein said four-quadrant photo detector 12 is placed on the back focal plane of astigmatic lens 11, and this road is reflective focusing light path;
Focusing control circuit is made of following each several part: four-quadrant photo detector 12 produces the output of four road electric signal, the amplifying circuit 13 that input end is connected with four road electrical signals of described four-quadrant photo detector 12, the filtering circuit 14 that input end links to each other with above-mentioned amplifying circuit 13 output terminals, the analog to digital conversion circuit 15 that input end links to each other with above-mentioned filtering circuit (14) output terminal, the digital signal processor 16 that input end links to each other with above-mentioned analog to digital conversion circuit 15 output terminals, the photoelectric isolation module circuit 18 that input end links to each other with above-mentioned digital signal processor 16 output terminals, the D/A converting circuit 19 that input end links to each other with above-mentioned photoelectric isolation module circuit 18 output terminals, the motor-driven power amplification circuit 20 that input end links to each other with above-mentioned D/A converting circuit 19 output terminals, the actuating motor 21 that input end links to each other with above-mentioned motor-driven power amplification circuit 20 output terminals, and the computing machine 17 that links to each other with above-mentioned digital signal processor 16 output terminals of input interface;
Focus adjusting mechanism is the leading screw-nut type mechanical focusing mechanism of joining with the actuating motor output shaft.
Feature of the present invention also is:
Described confocal light path and focusing light path contain successively by laser instrument 1, first pin hole 2, lens 3, two input paths with dichroic beamsplitter (4,5), object lens 6 and sample 8 formations at optical axis angle at 45, wherein laser instrument 1 is near first pin hole 2, first pin hole 2 is positioned on the front focal plane of lens 3, and sample 8 is placed on the back focal plane of object lens 6;
Along what the light path from sample 8 laser light reflected and the fluorescence institute approach that inspires set gradually be: described object lens 6, described dichroic beamsplitter 5, lens 3, pin hole 2 and photomultiplier 10, wherein said photomultiplier and second pin hole 2 are near placement, described second pin hole 2 is positioned on the back focal plane of lens 3, and this road is confocal light path;
Along what the light path from the laser of sample transmission and the fluorescence institute approach that inspires set gradually be: object lens 6, catoptron 4 and astigmatic lens 11, with four-quadrant photo detector 12, wherein said four-quadrant photo detector 12 is placed on the back focal plane of astigmatic lens 11, and this road is a transmission-type focusing light path;
Focusing control circuit is made of following each several part: four-quadrant photo detector 12 produces the output of four road electric signal, the amplifying circuit 13 that input end is connected with four road electrical signals of described four-quadrant photo detector 12, the filtering circuit 14 that input end links to each other with above-mentioned amplifying circuit 13 output terminals, the analog to digital conversion circuit 15 that input end links to each other with above-mentioned filtering circuit (14) output terminal, the digital signal processor 16 that input end links to each other with above-mentioned analog to digital conversion circuit 15 output terminals, the photoelectric isolation module circuit 18 that input end links to each other with above-mentioned digital signal processor 16 output terminals, the D/A converting circuit 19 that input end links to each other with above-mentioned photoelectric isolation module circuit 18 output terminals, the motor-driven power amplification circuit 20 that input end links to each other with above-mentioned D/A converting circuit 19 output terminals, the actuating motor 21 that input end links to each other with above-mentioned motor-driven power amplification circuit 20 output terminals, and the computing machine 17 that links to each other with above-mentioned digital signal processor 16 output terminals of input interface;
Focus adjusting mechanism is the leading screw-nut type mechanical focusing mechanism of joining with the actuating motor output shaft.
Its feature also is: actuating motor 21 is fixed on motor mounting plate 26 1 ends in the described mechanical focusing mechanism, the other end of described motor mounting plate 26 is fixed on mounting plate 24 tops, described actuating motor 21 is fixedlyed connected with leading screw 22 by shaft joint 30, described leading screw 22 links to each other with feed screw nut 29 with leading screw support 23 simultaneously, described leading screw support 23 other ends are fixed on the described mounting plate 24, described feed screw nut 29 is fixed on removable bottom 27 1 ends, the other end of described removable bottom 27 overlaps 7 fixed lenss 6 by object lens, described removable bottom 27 is fixedlyed connected with movable guiding rail 28, described movable guiding rail 28 links to each other with fixing slide block 25, have photoelectric sensor 31 on the described fixedly slide block 25, described fixedly slide block 25 other ends are fixed to described mounting plate 24 bottoms.Described actuating motor drives described leading screw by described shaft joint and rotates, described feed screw nut is moved up and down along described leading screw, be with described animal mirror cover and object lens to move up and down, arrive the target focal plane position that requires, finish the purpose of focusing up to sample along the optical axis direction.Photoelectric sensor plays location, spacing effect, avoids camera lens and sample to collide mutually.
Use proof: it has reached intended purposes.
Description of drawings
Fig. 1 and Fig. 2: the index path of two of optical system device embodiment in the laser cofocal scanner.
Fig. 3: the circuit theory block scheme of focusing control circuit.
Fig. 4: the mechanical principle block diagram of leading screw-nut type focus adjusting mechanism.
Embodiment:
Ask for an interview Fig. 1.Laser instrument 1 is near pin hole 2, pin hole 2 is placed on the front focal plane of lens 3, laser projects lens 3 through pin hole 2 and becomes directional light, through with the object lens 6 of optical axis angle at 45 dichroic beamsplitter 4, dichroic beamsplitter 5, large-numerical aperture vertically focal imaging to sample 8, object lens 6 are fixed on the object lens cover 7, sample 8 is placed on the specimen holder 9, excited sample 8 is sent fluorescence, scattering also takes place at this in laser simultaneously, form two-way light: reflected light and transmitted light, each road light all are made up of laser and the fluorescence that inspires.
In reflected light path, reflected light becomes directional light through object lens 6, behind the arrival dichroic beamsplitter 5, is formed reflected light and transmitted light by beam split once more.In reflected light path, reflected light is the fluorescence that ejects from sample, is reflexed on the lens 3 by dichroic beamsplitter 5, and focal imaging is to the preceding pin hole 2 of photomultiplier, and the photomultiplier tube 10 that is nestled up pin hole 2 detects the only confocal light path in this road; In transmitted light path, transmitted light is a laser light reflected from the sample, through dichroic beamsplitter 5, projects on the dichroic beamsplitter 4, and once more by beam split, wherein reflected light is reflected on the astigmatic lens 11, converges on the four-quadrant photo detector 12.Suitably select the focusing parameter of the both direction of astigmatic lens 11, can obtain certain focusing range and certain focusing accuracy.
In transmitted light path, transmitted light is through sample.
Goodbye Fig. 2.Laser instrument 1 is near pin hole 2, pin hole 2 is placed on the front focal plane of lens 3, laser projects lens 3 through pin hole 2 and becomes directional light, pass through that vertically focal imaging is to sample 8 with the object lens 6 of optical axis angle at 45 dichroic beamsplitter 5 and large-numerical aperture, object lens 6 are fixed on the object lens cover 7 again, and sample 8 is placed on the specimen holder 9, excited sample 8 is sent fluorescence, laser also at this scattering takes place simultaneously, forms two-way light: reflected light and transmitted light, each road light all are made up of laser and the fluorescence that inspires.In transmitted light path, transmitted light is through object lens 6 and catoptron 4, arrive astigmatic lens 11 at last and image on the four-quadrant photo detector 12, the focusing parameter when the both direction of selecting astigmatic lens 11 can obtain certain focusing range and certain focusing accuracy.
Goodbye Fig. 3.A branch of light that four-quadrant photo detector 12 will converge on it divides four regionally detectings, obtain the output of four road electric signal, enter digital signal processor 16 and computing machine 17 through amplifier 13, wave filter 14 and analog to digital converter 15, calculate and store the out of focus information of sample spot.According to this out of focus information, send the automatic focusing order by digital signal processor 16 or computing machine 17, remove to drive actuating motor 21 by photoelectric isolation module 18, digital to analog converter 19 and motor-driven power amplification circuit 20, drive focus adjusting mechanism and focus.
In Fig. 4, on one end of the actuating motor 21 fixed electrical machinery installing plates 26 in the focusing mechanical mechanism, the other end of described motor mounting plate 32 is fixed on the top of mounting plate 24, described actuating motor 21 is fixedlyed connected with leading screw 22 by shaft joint 30, described leading screw 22 links to each other with feed screw nut 29 with leading screw support 23 simultaneously, described leading screw support 23 other ends are fixed to the middle part of described mounting plate 24, described feed screw nut 29 is fixed on the end of removable bottom 27, the other end of described removable bottom 27 is by object lens fixed cover 7 fixed lenss 6, fixedly connected with movable guiding rail 28 in the middle part of described removable bottom 27, described movable guiding rail 28 links to each other with fixing slide block 25, have photoelectric sensor 31 on the described fixedly slide block 25, the other end of described fixedly slide block 25 is fixed to described mounting plate 24 bottoms.Motor 21 drives leading screw 22 rotations by shaft joint 30 like this, thereby feed screw nut 29 is moved up and down along leading screw 22, drive object lens cover 7 and object lens 6 and move up and down, arrive the object lens 6 back focal plane positions that require, finish focusing up to sample 8 along the optical axis direction.Photoelectric sensor 31 plays location, spacing effect, avoids camera lens 6 and sample 8 to collide mutually.
It can with the mode test sample of automatic focusing, have the advantage that focusing range is big, precision is high.
Claims (3)
1, the automatic focusing device of laser cofocal scanner contains confocal light path and focusing light path, and focusing control circuit and focus adjusting mechanism is characterized in that:
Described confocal light path and focusing light path contain successively by laser instrument (1), first pin hole (2), lens (3), two input path with dichroic beamsplitter (4,5), object lens (6) and sample (8) formation at optical axis angle at 45, wherein laser instrument (1) is near first pin hole (2), first pin hole (2) is positioned on the front focal plane of lens (3), and sample (8) is placed on the back focal plane of object lens (6);
Along what the light path from sample laser light reflected and the fluorescence institute approach that inspires set gradually be: described object lens (6), described dichroic beamsplitter (5), lens (3), second pin hole (2) and photomultiplier (10), wherein said photomultiplier (10) and second pin hole (2) are near placement, described second pin hole (2) is positioned on the back focal plane of lens (3), and this road is confocal light path;
Along what another light path from sample laser light reflected and the fluorescence institute approach that inspires set gradually be: described object lens (6), described two dichroic beamsplitters (5,4), astigmatic lens (11) and four-quadrant photo detector (12), wherein said four-quadrant photo detector (12) is placed on the back focal plane of astigmatic lens (11), and this road is reflective focusing light path;
Focusing control circuit is made of following each several part: four-quadrant photo detector (12) produces the output of four road electric signal, the amplifying circuit (13) that input end is connected with four road electrical signals of described four-quadrant photo detector (12), the filtering circuit (14) that input end links to each other with above-mentioned amplifying circuit (13) output terminal, the analog to digital conversion circuit (15) that input end links to each other with above-mentioned filtering circuit (14) output terminal, the digital signal processor (16) that input end links to each other with above-mentioned analog to digital conversion circuit (15) output terminal, the photoelectric isolation module circuit (18) that input end links to each other with above-mentioned digital signal processor (16) output terminal, the D/A converting circuit (19) that input end links to each other with above-mentioned photoelectric isolation module circuit (18) output terminal, the motor-driven power amplification circuit (20) that input end links to each other with above-mentioned D/A converting circuit (19) output terminal, the actuating motor (21) that input end links to each other with above-mentioned motor-driven power amplification circuit (20) output terminal, and the computing machine (17) that links to each other with above-mentioned digital signal processor (16) output terminal of input interface; Focus adjusting mechanism is the leading screw one nut type mechanical focusing mechanism of joining with the actuating motor output shaft.
2, the automatic focusing device of laser cofocal scanner contains confocal light path and focusing light path, and focusing control circuit and focus adjusting mechanism is characterized in that:
Described confocal light path and focusing light path contain successively by laser instrument (1), first pin hole (2), lens (3), two input path with dichroic beamsplitter (4,5), object lens (6) and sample (8) formation at optical axis angle at 45, wherein laser instrument (1) is near first pin hole (2), first pin hole (2) is positioned on the front focal plane of lens (3), and sample (8) is placed on the back focal plane of object lens (6);
Along what the light path from sample (8) laser light reflected and the fluorescence institute approach that inspires set gradually be: described object lens (6), described dichroic beamsplitter (5), lens (3), pin hole (2) and photomultiplier (10), wherein said photomultiplier and second pin hole (2) are near placement, described second pin hole (2) is positioned on the back focal plane of lens (3), and this road is confocal light path;
Along what the light path from the laser of sample transmission and the fluorescence institute approach that inspires set gradually be: object lens (6), catoptron (4) and astigmatic lens (11), and four-quadrant photo detector (12), wherein said four-quadrant photo detector (12) is placed on the back focal plane of astigmatic lens (11), and this road is a transmission-type focusing light path;
Focusing control circuit is made of following each several part: four-quadrant photo detector (12) produces the output of four road electric signal, the amplifying circuit (13) that input end is connected with four road electrical signals of described four-quadrant photo detector (12), the filtering circuit (14) that input end links to each other with above-mentioned amplifying circuit (13) output terminal, the analog to digital conversion circuit (15) that input end links to each other with above-mentioned filtering circuit (14) output terminal, the digital signal processor (16) that input end links to each other with above-mentioned analog to digital conversion circuit (15) output terminal, the photoelectric isolation module circuit (18) that input end links to each other with above-mentioned digital signal processor (16) output terminal, the D/A converting circuit (19) that input end links to each other with above-mentioned photoelectric isolation module circuit (18) output terminal, the motor-driven power amplification circuit (20) that input end links to each other with above-mentioned D/A converting circuit (19) output terminal, the actuating motor (21) that input end links to each other with above-mentioned motor-driven power amplification circuit (20) output terminal, and the computing machine (17) that links to each other with above-mentioned digital signal processor (16) output terminal of input interface; Focus adjusting mechanism is the leading screw-nut type mechanical focusing mechanism of joining with the actuating motor output shaft.
3, the automatic focusing device of laser cofocal scanner according to claim 1 and 2, it is characterized in that: actuating motor (21) is fixed on motor mounting plate (26) one ends in the described mechanical focusing mechanism, the other end of described motor mounting plate (26) is fixed on mounting plate (24) top, described actuating motor (21) is fixedlyed connected with leading screw (22) by shaft joint (30), described leading screw (22) links to each other with feed screw nut (29) with leading screw support (23) simultaneously, described leading screw support (23) other end is fixed on the described mounting plate (24), described feed screw nut (29) is fixed on removable bottom (27) one ends, the other end of described removable bottom (27) is by object lens cover (7) fixed lens (6), described removable bottom (27) is fixedlyed connected with movable guiding rail (28), described movable guiding rail (28) links to each other with fixing slide block (25), have photoelectric sensor (31) on the described fixedly slide block (25), described fixedly slide block (25) other end is fixed to described mounting plate (24) bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021002622A CN1176367C (en) | 2002-01-11 | 2002-01-11 | Automatic focus regulator for laser cofocal scanner |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021002622A CN1176367C (en) | 2002-01-11 | 2002-01-11 | Automatic focus regulator for laser cofocal scanner |
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| CN1358999A CN1358999A (en) | 2002-07-17 |
| CN1176367C true CN1176367C (en) | 2004-11-17 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101013136B (en) * | 2007-02-08 | 2011-07-20 | 北京工业大学 | Laser-induction fluorescence co-focusing scanning device and method |
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| CN100427923C (en) * | 2006-03-08 | 2008-10-22 | 东华大学 | Multifunction chip-detecting apparatus |
| CN101158645B (en) * | 2007-11-16 | 2010-06-09 | 北京工业大学 | Rotary type multichannel fluorescence excitation method based on input-output optical fiber |
| CN101158644B (en) * | 2007-11-16 | 2010-06-09 | 北京工业大学 | Rotary type multichannel fluorescentinducing method based on transmission optical fiber |
| CN103234629B (en) * | 2013-04-12 | 2015-05-13 | 中国科学院上海光学精密机械研究所 | Device for simultaneously measuring positions and angles of two optical beams on same incident plane |
| CN104976953B (en) * | 2015-06-26 | 2018-06-12 | 吉林大学 | Laser focuses on deviation detection device |
| CN106526799B (en) * | 2016-11-28 | 2019-03-01 | 河北汉光重工有限责任公司 | A kind of high stability, high energy laser receive camera lens |
| CN107219207B (en) * | 2017-07-04 | 2023-10-20 | 福州大学 | Automatic focusing method of CCD biochip fluorescence scanner |
| CN107991769B (en) * | 2018-01-12 | 2020-07-10 | 凝辉(天津)科技有限责任公司 | Two-dimensional scanning device |
| CN114442257B (en) * | 2022-01-25 | 2024-04-16 | 之江实验室 | Large-range high-precision light beam focal plane tracking device |
| CN114994892A (en) * | 2022-05-09 | 2022-09-02 | 中国科学院化学研究所 | Laser confocal microscopic imaging system and method |
-
2002
- 2002-01-11 CN CNB021002622A patent/CN1176367C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101013136B (en) * | 2007-02-08 | 2011-07-20 | 北京工业大学 | Laser-induction fluorescence co-focusing scanning device and method |
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