CN117629724A - Full-automatic BOD measurement 5 Apparatus and method of (a) - Google Patents
Full-automatic BOD measurement 5 Apparatus and method of (a) Download PDFInfo
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- CN117629724A CN117629724A CN202210966721.2A CN202210966721A CN117629724A CN 117629724 A CN117629724 A CN 117629724A CN 202210966721 A CN202210966721 A CN 202210966721A CN 117629724 A CN117629724 A CN 117629724A
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- 238000005259 measurement Methods 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title abstract description 16
- 239000012895 dilution Substances 0.000 claims abstract description 150
- 238000010790 dilution Methods 0.000 claims abstract description 150
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 140
- 230000033001 locomotion Effects 0.000 claims abstract description 67
- 230000029087 digestion Effects 0.000 claims abstract description 40
- 239000007788 liquid Substances 0.000 claims abstract description 40
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 26
- 238000010438 heat treatment Methods 0.000 claims abstract description 15
- 239000000523 sample Substances 0.000 claims description 95
- 239000000243 solution Substances 0.000 claims description 41
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 36
- 229910052760 oxygen Inorganic materials 0.000 claims description 36
- 239000001301 oxygen Substances 0.000 claims description 36
- 239000012496 blank sample Substances 0.000 claims description 19
- 239000011259 mixed solution Substances 0.000 claims description 9
- 238000007865 diluting Methods 0.000 claims description 4
- 238000011156 evaluation Methods 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 claims description 2
- 238000000691 measurement method Methods 0.000 abstract description 5
- 238000010586 diagram Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
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Abstract
The invention relates to a full-automatic BOD measurement method 5 Comprises: an incubator; the automatic dilution system is arranged in the incubator; the automatic dilution system comprises a water sample bottle, a dilution bottle, a cover opening device, a suction pipe and a first movement device connected with the suction pipe, wherein the first movement device is connected with the measurement device; the dilution ratio automatic judging system is matched with the automatic dilution system; the dilution ratio automatic judging system comprises a digestion measuring device and a reagent bottle for containing a measuring reagent, wherein the digestion measuring device comprises a second moving device, a liquid adding port and a digestion tube, the second moving device is connected with the liquid adding port, the liquid adding port is communicated with a suction tube, and the digestion tube is matched with a heating device. The invention also relates to a full-automatic BOD measurement method 5 Is a method of (2). The invention can rapidly, accurately and automatically judge the dilution ratio, accurately and automatically dilute, realize the automation of culture and automatically and accurately measure BOD 5 Values.
Description
Technical Field
The invention relates to the technical field of water quality monitoring, in particular to a full-automatic BOD measurement method 5 Is provided.
Background
BOD 5 (five-day Biochemical oxygen demand) refers to dissolved oxygen in water consumed in a biochemical process in which microorganisms decompose certain organic substances in water within five days under prescribed conditions, which is an important index for determining organic pollutants in water.
Currently, laboratories measure BOD 5 The method of (2) is a dilution inoculation method. In this method, an operator first needs to empirically predict BOD of a water sample 5 And then determining whether to dilute. If the water sample needs to be diluted, the Chemical Oxygen Demand (COD) of the water sample needs to be measured, and then the multiple of the required dilution is calculated according to the COD to carry out manual dilution. During dilution, the water used for dilution is aerated to saturate dissolved oxygen in the water, and then the dilution water is used for diluting the water sample. Inoculating microorganism-free water sample, and measuring dissolved oxygen ρ of diluted water sample by titration or electrode method 1 Sealing the water sample, culturing in an incubator for 5 days, and measuring the dissolved oxygen rho of the water sample after 5 days 2 Finally according to ρ 1 And ρ 2 Calculating BOD of the original water sample by the difference value 5 Values.
However, the dilution and inoculation method requires manual operation, and it is difficult to obtain accurate and stable experimental results.
Disclosure of Invention
The invention aims to overcome the technical defects and provide a full-automatic BOD measurement 5 The device and the method can realize automatic and accurate BOD measurement 5 Values.
The technical scheme for achieving the purpose is as follows:
the invention provides a full-automatic BOD measurement method 5 Comprises:
an incubator;
the automatic dilution system is arranged in the incubator; the automatic dilution system comprises a plurality of water sample bottles for containing water samples, a plurality of dilution bottles, a cover opening device for opening and/or closing the water sample bottles and bottle caps of the dilution bottles, a suction pipe for sucking the water samples and/or the dilution water and a first movement device connected with the suction pipe, wherein the first movement device is connected with a measuring device;
the dilution ratio automatic judging system is matched with the automatic dilution system; the dilution ratio automatic judging system comprises a digestion measuring device and a reagent bottle for containing a measuring reagent, wherein the digestion measuring device comprises a second moving device, a liquid adding port and a digestion tube for containing a water sample, the second moving device is connected with the liquid adding port, the liquid adding port is communicated with the suction tube, and the digestion tube is matched with a heating device.
Optionally, the automatic dilution system is configured to: the first motion device controls the movement of the suction pipe, water samples are taken out of the water sample bottle and added into the dilution bottle, and dilution water is taken out of the dilution bucket and added into the dilution bottle.
Optionally, the measuring device is a dissolved oxygen measuring electrode.
Optionally, the dilution ratio automatic judging system is configured to: the second motion device controls the movement of the liquid adding port, and the water sample is added into the digestion tube through the liquid adding port; taking out the measuring reagent from the reagent bottle, and controlling the movement of the liquid adding port by the second movement device, and adding the measuring reagent to the digestion tube through the liquid adding port; the heating device is used for heating and digesting the mixed solution in the digestion tube so as to measure the COD value; and determining the multiple of the required dilution according to the COD value.
The invention also provides a full-automatic BOD measurement device 5 Comprises the following steps:
step S1, providing a fully automated BOD measurement as claimed in claims 1-4 5 Is a device of (2)
S2, placing a water sample bottle and a dilution bottle for containing a water sample to be tested into an automatic dilution system in an incubator;
s3, acquiring the BOD of the water sample to be detected by using an automatic dilution system and an automatic dilution ratio judging system 5 And calculating the multiple C of dilution required by the water sample to be detected;
s4, diluting the water sample to be detected by using the automatic dilution system according to the multiple C, the multiple xC and the multiple C/x to obtain a culture solution, wherein x is a preset constant;
s5, obtaining an initial dissolved oxygen value of the culture solution by using the measuring device;
s6, obtaining an initial dissolved nutrient value of the blank sample by using the automatic dilution system and the measuring device;
step S6, after the culture solution and the blank are cultured for 5 days at the constant temperature of 20 ℃, respectively obtaining the dissolved oxygen values of the culture solution and the blank after five days according to the automatic dilution system and the measuring device;
step S7, obtaining BOD of the water sample to be detected according to the initial dissolved oxygen values of the culture solution and the blank sample and the five-day dissolved oxygen values of the culture solution and the blank sample 5 Values.
Optionally, the step S3 includes:
step S31, a first movement device of the automatic dilution system controls the cover opening device to move to the upper part of the water sample bottle;
step S32, the cover opening device of the automatic dilution system opens the bottle cover of the water sample bottle;
step S33, the first movement device controls the suction pipe to be inserted into the water sample bottle;
step S34, sucking the water sample to be detected through a liquid feeding port by a suction pipe;
step S35, the second movement device controls the movement of the liquid adding port, and the water sample to be detected is added into the digestion tube through the liquid adding port;
step S36, the second movement device controls the liquid adding port to take out the measuring reagent from the reagent bottle, and the measuring reagent is added into the digestion tube through the liquid adding port;
step S37, heating and digestion are carried out on the mixed solution in the digestion tube by the heating device;
step S38, after digestion is finished, measuring the COD value;
step S39, according to the COD value and BOD 5 Obtaining the BOD of the water sample to be detected 5 Is determined by the pre-evaluation value of (2);
step S310, BOD to be obtained 5 Is divided by the predicted value of (2)And determining the multiple C of the dilution required by the water sample through a test value.
Alternatively, the empirical value ranges from 2 to 8.
Optionally, the step S4 includes:
step S41, opening a bottle cap of a dilution bottle by a cover opening device of an automatic dilution system;
step S42, according to the dilution multiple, the first motion device controls the suction pipe to suck the water sample to be detected from the water sample bottle and respectively to the corresponding dilution bottle; taking the original water sample bottle containing the water sample as a dilution bottle with the multiple of C/2 after taking the water sample, and discharging the residual water sample;
in step S43, the first motion device controls the suction pipe to suck the dilution water from the dilution water bucket, and the dilution bottles containing the water sample to be tested are filled with the dilution water respectively, and the mixed solution contained in the bottles is the culture solution.
Optionally, the step S5 includes:
step S51, the first movement device controls the suction pipe to be pulled out of the culture solution, controls the measuring device to be inserted into the culture solution, and measures the initial dissolved oxygen value of the culture solution;
in step S52, the first movement device controls the measuring device to lift from the culture solution and separate from the dilution bottle containing the culture solution.
Optionally, the step S6 includes:
step S61, a first motion device of the automatic dilution system controls the suction pipe to suck dilution water from the dilution water bucket to fill a dilution bottle, and the dilution water contained in the bottle is used as a blank sample;
step S62, the first movement device controls the suction pipe to be pulled out of the blank sample, controls the measuring device to be inserted into the blank sample, and measures the initial dissolved oxygen value of the blank sample;
step S63, the first movement device controls the measuring device to lift up from the blank and leave a dilution bottle containing the blank;
in step S64, the cap opening device closes the caps of the respective bottles.
The invention is allAutomatic BOD measurement 5 The device and the method have the beneficial effects that:
the invention can rapidly, accurately and automatically judge the dilution ratio, accurately and automatically dilute, and can realize the automation of culture, thereby automatically and accurately measuring BOD 5 Values.
Drawings
FIG. 1 shows a fully automated BOD measurement according to the invention 5 Is a schematic structural diagram of the device.
Fig. 2 and 3 are schematic structural views of the automatic dilution system in fig. 1.
Fig. 4 is a schematic diagram of a structure of the automatic dilution ratio judging system in fig. 1.
Detailed Description
The invention will be further described with reference to the drawings and the specific examples.
As shown in FIG. 1, the invention provides a full-automatic BOD measurement method 5 Comprises an incubator 1, an automatic dilution system 2 arranged in the incubator 1, and an automatic dilution ratio judging system 3 matched with the automatic dilution system 2.
As shown in fig. 2 and 3, the automatic dilution system 2 comprises a plurality of water sample bottles 21 for holding water samples, a plurality of dilution bottles 22, a cover opening device 23 for opening and/or closing the caps of the water sample bottles 21 and the dilution bottles 22, a suction pipe 24 for sucking water samples and/or dilution water, and a first movement device 25 connected to the suction pipe 24. Specifically, the automatic dilution system 2 is configured to: the first movement means 25 controls the movement of the suction pipe 24, takes out a certain amount of water sample from the water sample bottle 21 to the dilution bottle 22, and transfers a certain amount of dilution water from the dilution water bucket (not shown) to the dilution bottle 22, thereby achieving the purpose of automatic dilution.
In addition, the first movement device 25 is also connected to a measuring device 4, and the measuring device 4 is a dissolved oxygen measuring electrode for measuring the dissolved oxygen value in each water sample bottle 21.
Referring to fig. 1 and 4, the dilution ratio automatic determination system 3 includes a digestion measurement apparatus 31 and a reagent bottle 32 containing a measurement reagent. The digestion measurement device 31 comprises a second movement device 311, a liquid adding port 312 and a digestion tube 313 for containing water samples, wherein the second movement device 311 is connected with the liquid adding port 312, the liquid adding port 312 is communicated with the suction tube 24, and the digestion tube 313 is matched with the heating device 315 for heating the water samples in the digestion tube 313. Specifically, the dilution ratio automatic determination system 3 is configured to: the liquid adding port 312 is used for absorbing a certain amount of water sample through the suction pipe 24, the second movement device 311 is used for controlling the movement of the liquid adding port 312, and the water sample is added into the digestion pipe 313 through the liquid adding port 312; then taking out a certain amount of measuring reagent from the reagent bottle 32, and controlling the movement of the liquid adding port 312 by the second movement device 311, and adding the measuring reagent to the digestion tube 313 through the liquid adding port 312; then, the heating device 315 heats and digests the mixed solution in the digestion pipe 313 to measure the COD value; and finally, determining the multiple of dilution required by the water sample according to the COD value.
The first movement device 25 and the second movement device 311 are both three-dimensional mechanical movement devices. For example, the first moving means 25 includes an X-direction moving motor 251, a Y-direction moving motor 252, and a Z-direction moving motor 253, and X, Y, and Z directions refer to directions in a three-dimensional cartesian coordinate system.
Under the normal condition, the content of organic matters in the water sample to be detected is more, BOD 5 The mass concentration of the water sample to be measured is larger than 6mg/L, and the water sample to be measured is measured after being diluted properly. Without knowing the specific BOD 5 Under the condition of (1), the COD value in the water sample needs to be measured so as to judge the BOD in the water sample 5 And then determine the dilution factor. Therefore, BOD is measured based on the above-mentioned full automation 5 The invention also provides a device for fully automatically measuring BOD 5 The method comprises the following steps:
step S1, providing the fully automatic BOD measurement 5 Is provided with a device for the control of the flow of air,
step S2, placing a water sample bottle 21 and a dilution bottle 22 for containing a water sample to be tested in the automatic dilution system 2 in the incubator 1.
Step S3, obtaining BOD of the water sample to be detected by utilizing the automatic dilution system 2 and the dilution ratio automatic judging system 3 5 And calculating the multiple C of dilution required by the water sample to be measured. Specifically, the method comprises the following steps:
step S31, the first movement device 25 of the automatic dilution system 2 controls the cover opening device 23 to move to the upper part of the water sample bottle 21;
step S32, the cover opening device 23 of the automatic dilution system 2 opens the bottle cover of the water sample bottle 21;
step S33, the first movement device 25 controls the suction pipe 24 to be inserted into the water sample bottle 21;
step S34, sucking a certain amount of water sample to be detected by the suction pipe 24 through the liquid filling port 312;
step S35, the second movement device 311 controls the movement of the liquid adding port 312, and the water sample to be measured is added into the digestion tube 313 through the liquid adding port 312;
step S36, taking out a certain amount of measuring reagent from the reagent bottle 32, controlling the liquid adding port 312 to move by the second movement device 311, and adding the measuring reagent into the digestion tube 313 through the liquid adding port 312;
step S37, the heating device 315 heats and digests the mixed solution in the digestion pipe 313;
step S38, after digestion is finished, measuring the COD value;
step S39, according to the COD value and BOD 5 Is used for obtaining the BOD of the water sample to be detected 5 Is determined by the pre-evaluation value of (2);
step S310, BOD to be obtained 5 Dividing the predicted value of (2) by an empirical value to determine the multiple C of the dilution required by the water sample. Wherein the empirical value ranges from 2 to 8.
And S4, diluting the water sample to be detected by using the automatic dilution system 2 according to the multiple C, the multiple xC and the multiple C/x to obtain a culture solution. Wherein x is a predetermined constant, and can be selected as required. In this embodiment, x takes a value of 2.
Due to the measured COD value and the actual BOD 5 The correlation of the values is not determined, so three fold dilutions are made in the present invention.
Specifically, the method comprises the following steps:
step S41, the cover opening device 23 of the automatic dilution system 2 opens the cover of the dilution bottle 22;
step S42, according to the dilution multiple, the first movement device 25 of the automatic dilution system 2 controls the suction pipe 24 to suck the water sample to be measured from the water sample bottle 21 into the corresponding dilution bottle 22 respectively; after the water sample is taken, the original water sample bottle 21 is used as a dilution bottle with multiple C/2, and the rest water sample is discharged.
In step S43, the first motion device 25 controls the suction pipe 24 to suck the dilution water from the dilution water tank (not shown) and fill the dilution bottles with the water sample to be tested, respectively, and the mixed solution in the bottles is the culture solution.
Step S5, obtaining an initial dissolved oxygen value of the culture solution by using the measuring device 4. Specifically, the method comprises the following steps:
step S51, the first movement device 25 controls the suction pipe 24 to be pulled out from the culture solution, controls the measuring device 4 to be inserted into the culture solution, and measures the initial dissolved oxygen value of the culture solution;
step S52, the first movement device 25 controls the measuring device 4 to lift up from the culture solution and leave the dilution bottle containing the culture solution;
step S6, an initial dissolved oxygen value of the blank sample is obtained by using the automatic dilution system 2 and the measuring device 4. Specifically, the method comprises the following steps:
step S61, the first motion device 25 of the automatic dilution system 2 controls the suction pipe 24 to suck the dilution water from the dilution water bucket (not shown) to fill a dilution bottle 22, and the dilution water contained in the bottle is used as a blank sample;
step S62, the first movement device 25 controls the suction pipe 24 to be pulled out from the blank, controls the measuring device 4 to be inserted into the blank, and measures the initial dissolved oxygen value of the blank;
step S63, the first movement device 25 controls the measurement device 4 to lift up from the blank and leave the dilution bottle 22 containing the blank;
in step S64, the cap opening device 23 closes the caps of the respective bottles. And S6, after the culture solution and the blank are cultured for 5 days at the constant temperature of 20 ℃, obtaining the dissolved oxygen values of the culture solution and the blank after five days according to the automatic dilution system 2 and the measuring device 4.
It should be noted that, the specific operation steps of the automatic dilution system 2 and the measuring device 4 in the step S6 may refer to the detailed descriptions of the steps S51 to S52 in the step S5 and the steps S61 to S63 in the step S6, which are not repeated herein.
Step S7, according to the initial dissolved oxygen values of the culture solution and the blank sample and the culture solution and the blank sampleAfter five days, the dissolved oxygen value is obtained to obtain BOD of the water sample to be detected 5 Values.
If the water sample to be detected is undiluted, BOD is calculated according to the following formula 5 Value ρ:
ρ＝ρ 1 -ρ 2
wherein ρ is 1 The mass concentration of dissolved oxygen of the water sample (namely culture solution) to be detected before five days is represented by ρ 2 And the mass concentration of the dissolved oxygen of the water sample to be measured after five days is shown.
If the water sample to be measured is diluted, BOD is calculated according to the following formula 5 Value ρ':
wherein ρ is 1 ' represents the mass concentration of dissolved oxygen in the culture solution five days ago, ρ 2 ' represents the mass concentration of dissolved oxygen in the culture solution after five days, ρ 3 Represents the mass concentration of dissolved oxygen, ρ, of the blank sample five days ago 4 Represents the mass concentration of dissolved oxygen, f, of the blank sample after five days 1 Represents the proportion of the dilution water in the culture solution, f 2 The ratio of the original water sample to be measured before dilution in the culture solution is shown.
The invention provides a full-automatic BOD measurement device which is suitable for laboratory use and is easy to operate 5 The device and the method can automatically judge the dilution ratio, automatically dilute, automatically cultivate, automatically measure the oxygen content, automatically output a report and accurately detect the result.
The present invention has been described in detail with reference to the embodiments of the drawings, and those skilled in the art can make various modifications to the invention based on the above description. Accordingly, certain details of the illustrated embodiments are not to be taken as limiting the invention, which is defined by the appended claims.
Claims (10)
1. Full-automatic BOD measurement 5 Is characterized by comprising:
an incubator;
the automatic dilution system is arranged in the incubator; the automatic dilution system comprises a plurality of water sample bottles for containing water samples, a plurality of dilution bottles, a cover opening device for opening and/or closing the water sample bottles and bottle caps of the dilution bottles, a suction pipe for sucking the water samples and/or the dilution water and a first movement device connected with the suction pipe, wherein the first movement device is connected with a measuring device;
the dilution ratio automatic judging system is matched with the automatic dilution system; the dilution ratio automatic judging system comprises a digestion measuring device and a reagent bottle for containing a measuring reagent, wherein the digestion measuring device comprises a second moving device, a liquid adding port and a digestion tube for containing a water sample, the second moving device is connected with the liquid adding port, the liquid adding port is communicated with the suction tube, and the digestion tube is matched with a heating device.
2. The fully automated BOD measurement of claim 1 5 Is characterized in that the automatic dilution system is arranged to: the first motion device controls the movement of the suction pipe, water samples are taken out of the water sample bottle and added into the dilution bottle, and dilution water is taken out of the dilution bucket and added into the dilution bottle.
3. The fully automated BOD measurement of claim 1 5 Is characterized in that the measuring device is a dissolved oxygen measuring electrode.
4. The fully automated BOD measurement of claim 1 5 Wherein the dilution ratio automatic judging system is configured to: the second motion device controls the movement of the liquid adding port, and the water sample is added into the digestion tube through the liquid adding port; taking out the measuring reagent from the reagent bottle, and controlling the movement of the liquid adding port by the second movement device, and adding the measuring reagent to the digestion tube through the liquid adding port; the heating device is used for heating and digesting the mixed solution in the digestion tube so as to measure the COD value; based on the value of the COD value of the water,the multiple of the required dilution is determined.
5. Full-automatic BOD measurement 5 Is characterized in that it comprises:
step S1, providing a fully automated BOD measurement as claimed in claims 1-4 5 Is a device of (2)
S2, placing a water sample bottle and a dilution bottle for containing a water sample to be tested into an automatic dilution system in an incubator;
s3, acquiring the BOD of the water sample to be detected by using an automatic dilution system and an automatic dilution ratio judging system 5 And calculating the multiple C of dilution required by the water sample to be detected;
s4, diluting the water sample to be detected by using the automatic dilution system according to the multiple C, the multiple xC and the multiple C/x to obtain a culture solution, wherein x is a preset constant;
s5, obtaining an initial dissolved oxygen value of the culture solution by using the measuring device;
s6, obtaining an initial dissolved nutrient value of the blank sample by using the automatic dilution system and the measuring device;
step S6, after the culture solution and the blank are cultured for 5 days at the constant temperature of 20 ℃, respectively obtaining the dissolved oxygen values of the culture solution and the blank after five days according to the automatic dilution system and the measuring device;
step S7, obtaining BOD of the water sample to be detected according to the initial dissolved oxygen values of the culture solution and the blank sample and the five-day dissolved oxygen values of the culture solution and the blank sample 5 Values.
6. The fully automatic BOD measurement of claim 5 5 Is characterized in that said step S3 comprises:
step S31, a first movement device of the automatic dilution system controls the cover opening device to move to the upper part of the water sample bottle;
step S32, the cover opening device of the automatic dilution system opens the bottle cover of the water sample bottle;
step S33, the first movement device controls the suction pipe to be inserted into the water sample bottle;
step S34, sucking the water sample to be detected through a liquid feeding port by a suction pipe;
step S35, the second movement device controls the movement of the liquid adding port, and the water sample to be detected is added into the digestion tube through the liquid adding port;
step S36, taking out the measuring reagent from the reagent bottle, wherein the second movement device controls the liquid adding port to move, and the measuring reagent is added into the digestion tube through the liquid adding port;
step S37, heating and digestion are carried out on the mixed solution in the digestion tube by the heating device;
step S38, after digestion is finished, measuring the COD value;
step S39, according to the COD value and BOD 5 Obtaining the BOD of the water sample to be detected 5 Is determined by the pre-evaluation value of (2);
step S310, obtaining the BOD 5 Dividing the predicted value of (2) by an empirical value to determine the multiple C of the dilution required by the water sample.
7. The fully automated BOD measurement of claim 6 5 Is characterized in that the empirical value ranges from 2 to 8.
8. The fully automated measurement of BOD of claim 7 5 Is characterized in that said step S4 comprises:
step S41, opening a bottle cap of a dilution bottle by a cover opening device of an automatic dilution system;
step S42, according to the dilution multiple, the first motion device controls the suction pipe to suck the water sample to be detected from the water sample bottle and respectively to the corresponding dilution bottle; taking the original water sample bottle containing the water sample as a dilution bottle with the multiple of C/2 after taking the water sample, and discharging the residual water;
in step S43, the first motion device controls the suction pipe to suck the dilution water from the dilution water bucket, and the dilution bottles containing the water sample to be tested are filled with the dilution water respectively, and the mixed solution contained in the bottles is the culture solution.
9. The fully automated measurement of BOD of claim 8 5 Is characterized in that said step S5 comprises:
step S51, the first movement device controls the suction pipe to be pulled out of the culture solution, controls the measuring device to be inserted into the culture solution, and measures the initial dissolved oxygen value of the culture solution;
in step S52, the first movement device controls the measuring device to lift from the culture solution and separate from the dilution bottle containing the culture solution.
10. The fully automated measurement of BOD of claim 9 5 Is characterized in that said step S6 comprises: step S61, a first motion device of the automatic dilution system controls the suction pipe to suck dilution water from the dilution water bucket to fill a dilution bottle, and the dilution water contained in the bottle is used as a blank sample;
step S62, the first movement device controls the suction pipe to be pulled out of the blank sample, controls the measuring device to be inserted into the blank sample, and measures the initial dissolved oxygen value of the blank sample;
step S63, the first movement device controls the measuring device to lift up from the blank and leave a dilution bottle containing the blank;
in step S64, the cap opening device closes the caps of the respective bottles.
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| CN202210966721.2A CN117629724A (en) | 2022-08-12 | 2022-08-12 | Full-automatic BOD measurement 5 Apparatus and method of (a) |
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| CN202210966721.2A CN117629724A (en) | 2022-08-12 | 2022-08-12 | Full-automatic BOD measurement 5 Apparatus and method of (a) |
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