CN117617237A - Mosquito-killing young slow-release formulation and application thereof - Google Patents
Mosquito-killing young slow-release formulation and application thereof Download PDFInfo
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- CN117617237A CN117617237A CN202311291987.2A CN202311291987A CN117617237A CN 117617237 A CN117617237 A CN 117617237A CN 202311291987 A CN202311291987 A CN 202311291987A CN 117617237 A CN117617237 A CN 117617237A
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- biochar
- mosquito
- sodium alginate
- killing
- slow
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- 239000000203 mixture Substances 0.000 title claims abstract description 21
- 238000009472 formulation Methods 0.000 title claims abstract description 17
- 239000000575 pesticide Substances 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- 230000002147 killing effect Effects 0.000 claims abstract description 26
- 239000003814 drug Substances 0.000 claims abstract description 24
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 21
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 21
- 239000000661 sodium alginate Substances 0.000 claims abstract description 21
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 21
- 229940079593 drug Drugs 0.000 claims abstract description 17
- 239000002917 insecticide Substances 0.000 claims abstract description 16
- 241000255925 Diptera Species 0.000 claims abstract description 12
- 238000004132 cross linking Methods 0.000 claims abstract description 10
- 239000004480 active ingredient Substances 0.000 claims abstract description 7
- 241000256113 Culicidae Species 0.000 claims abstract description 3
- 239000000725 suspension Substances 0.000 claims description 18
- 239000000843 powder Substances 0.000 claims description 16
- 238000013268 sustained release Methods 0.000 claims description 14
- 239000012730 sustained-release form Substances 0.000 claims description 14
- YKBZOVFACRVRJN-UHFFFAOYSA-N dinotefuran Chemical compound [O-][N+](=O)\N=C(/NC)NCC1CCOC1 YKBZOVFACRVRJN-UHFFFAOYSA-N 0.000 claims description 13
- 239000002552 dosage form Substances 0.000 claims description 12
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 11
- 239000007864 aqueous solution Substances 0.000 claims description 11
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 11
- 239000012498 ultrapure water Substances 0.000 claims description 11
- 238000011068 loading method Methods 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 9
- 239000001110 calcium chloride Substances 0.000 claims description 7
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 7
- 235000011148 calcium chloride Nutrition 0.000 claims description 7
- 241000256111 Aedes <genus> Species 0.000 claims description 6
- 230000008014 freezing Effects 0.000 claims description 6
- 238000007710 freezing Methods 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 239000008367 deionised water Substances 0.000 claims description 4
- 229910021641 deionized water Inorganic materials 0.000 claims description 4
- 239000003094 microcapsule Substances 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 239000007900 aqueous suspension Substances 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 230000000749 insecticidal effect Effects 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 239000000077 insect repellent Substances 0.000 claims 5
- 229930192000 nocardione Natural products 0.000 claims 1
- 238000009395 breeding Methods 0.000 abstract description 6
- 230000001488 breeding effect Effects 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 239000004615 ingredient Substances 0.000 abstract description 4
- 241000282414 Homo sapiens Species 0.000 abstract description 2
- 241000124008 Mammalia Species 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 231100000636 lethal dose Toxicity 0.000 abstract description 2
- 230000000361 pesticidal effect Effects 0.000 abstract description 2
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 description 28
- 239000011734 sodium Substances 0.000 description 23
- 229910052708 sodium Inorganic materials 0.000 description 23
- 238000012360 testing method Methods 0.000 description 15
- 241000256173 Aedes albopictus Species 0.000 description 12
- 238000002360 preparation method Methods 0.000 description 10
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
- 230000001186 cumulative effect Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 230000002045 lasting effect Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 239000010902 straw Substances 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 230000005923 long-lasting effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000002688 persistence Effects 0.000 description 2
- 239000003090 pesticide formulation Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 125000001741 organic sulfur group Chemical group 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000002728 pyrethroid Substances 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
- A01N25/10—Macromolecular compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/26—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests in coated particulate form
- A01N25/28—Microcapsules or nanocapsules
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/04—Insecticides
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- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Toxicology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Chemical & Material Sciences (AREA)
- Insects & Arthropods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a mosquito-killing young slow-release formulation, which comprises the following active ingredients, wherein the first ingredient is biochar loaded with insecticide, and the second ingredient is three-dimensional grid gel formed by crosslinking sodium alginate and Ca2+, and is applied to various small and medium-sized water bodies suitable for mosquito larva growth. The invention provides a mosquito killing young slow release formulation, the drug in the slow release formulation can be released continuously while playing a role rapidly, the effective lethal concentration can be maintained for a long time, the comprehensive effect of killing mosquito larvae in the breeding place is further improved, the pesticide in the formulation can be released slowly into the water of the breeding place according to the preset time, the pesticide effect can be maintained for a long time, the carrier can protect the drug from the influence of repeated drought and sunlight irradiation while the duration is prolonged, the utilization rate is high, and the invention has the characteristics of convenient application, environmental protection and safety to non-target organisms, especially human beings and other mammals.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a mosquito-killing young slow release formulation and application thereof.
Background
Pesticides refer to chemical agents used to control pests. Including organic pesticides (organic chlorine, organic phosphorus, organic sulfur preparation and carbamate and pyrethroid), inorganic pesticides (inorganic arsenic, inorganic fluorine, inorganic sulfur preparation), vegetable pesticides, mineral oil pesticides, microbial pesticides. The pesticide is the most used and the most variety of pesticide, and most of pesticide can only kill insects but not prevent diseases. The pesticide plays an extremely important role in solving the grain problem of human beings for agricultural yield increase, but is also the most important factor causing the environment pollution of pesticides (such as stable chemical property, difficult degradation and easy accumulation in soil and organisms), and has close relationship with the reduction or even imminent extinction of natural enemies of pests and other beneficial organisms;
at present, the use of chemical pesticides is still a main means for controlling aedes, the conventionally used pesticide dosage forms and pesticide application methods enable the pesticide to have low utilization rate, and especially under the conditions of more rainwater and strong sunlight in south China, outdoor open-air pesticide spraying has short lasting effect, wherein most of pesticide active ingredients are easy to deactivate under the irradiation of ultraviolet rays, the active ingredients are unevenly distributed when the chemical pesticide is sprayed and applied, and the chemical pesticide is not suitable for the repeated drought-ponding conditions of water bodies of mosquito breeding places and has the obvious defects of short storage period and the like. In addition, in most cases, the population density of aedes increases with the increase of precipitation, but after urban disinfection, many pesticides are often washed by rainwater and then enter a sewer system along with water flow, and the aedes do not play a role in target breeding places and even pollute the environment. The result of ineffective disinfection or reduced efficacy is that the mosquito is not killed, the generation of drug resistance is further stimulated under the sublethal dose, and meanwhile, irreparable damage is caused to the ecological environment, so that a mosquito-killing young slow release formulation is needed to solve the problems.
Disclosure of Invention
In view of the above-mentioned problems with the background art, the present invention has as its object: aims at providing a mosquito-killing young slow release formulation and application thereof.
In order to achieve the technical purpose, the invention adopts the following technical scheme:
the slow released mosquito killing prepn consists of two components, including biochar for loading pesticide and three-dimensional grid gel formed by crosslinking sodium alginate and Ca2+;
the insecticidal composition is prepared by the following method:
s1: adding the biochar into deionized water according to a proportion, fully stirring to obtain a biochar suspension, then adding pesticide powder, and continuously stirring until the powder is completely dissolved and adsorbed to obtain the biochar suspension containing the pesticide, wherein the biochar suspension is used for preparing a slow-release dosage form subsequently;
s2: fully mixing biochar suspension containing insecticide with sodium alginate aqueous solution (stirring for not less than 12 hours), pouring into a mould, freezing and fixing for 1 hour at the temperature of minus 20 ℃, freezing into solid, pouring into a prepared calcium chloride aqueous solution, standing for 0.5 hour at room temperature to perform cross-linking reaction, filtering, washing and drying to form the black cylindrical microcapsule.
Further defined, in the first group, the insecticide moiety is one or more of the third generation nicotinic insecticide dinotefuran and the natural insecticide nocardianone.
Further defined, the sodium alginate is prepared from sodium alginate powder and ultrapure water according to a ratio of 1:49, and the finished product has certain elasticity and gel strength after crosslinking in the proportion; the biochar suspension is prepared by mixing biochar powder and ultrapure water according to a ratio of 1:9, in this ratio, can make the drug-loading rate and release rate reach optimum ratio
Further defined, the content of biochar in the aqueous suspension of biochar is 1g/9mL; the mass fraction of sodium alginate in the sodium alginate aqueous solution is 2%; the mass fraction of CaCl2 in the calcium chloride solution is 2%.
Further defined, the mosquito-killing young slow release formulation is used for controlling aedes intermedium.
The slow released mosquito killing preparation is applied to various small and medium water bodies suitable for mosquito larva to grow.
The beneficial effects of the invention are as follows:
1. the sodium alginate-biochar slow release agent can control the release speed of the medicine, prolong the duration of the medicine, and reduce the medicine application times and manpower and material resources;
2. the biochar has good adsorption performance, can load more dosage, and can avoid burst release phenomenon;
3. has better killing effect on aedes albopictus larvae, and has better quick-acting property and lasting effect;
the invention provides a sodium alginate-biochar microcapsule slow-release pesticide formulation, which enables the drug in the slow-release formulation to be released continuously while taking effect rapidly, maintains effective lethal concentration for a long time, further improves the comprehensive effect of killing mosquito larvae in the breeding place, and enables the pesticide in the formulation to be released slowly into the water of the breeding place according to preset time, the pesticide effect of the pesticide can be maintained for a long time, the carrier can protect the drug from being influenced by repeated drought and sunlight irradiation while prolonging the lasting period, the utilization rate is high, and the pesticide formulation has the characteristics of convenient application, environmental friendliness and safety to non-target organisms, especially people and other mammals.
Drawings
The invention can be further illustrated by means of non-limiting examples given in the accompanying drawings;
FIG. 1 is a graph of initial drug loading versus slow release rate for various embodiments of the present invention:
FIG. 2 is a graph showing the effect of temperature on the release of the active ingredient of the sustained-release agent according to the embodiment of the present invention;
FIG. 3 is a graph showing a quick-acting test for killing Aedes albopictus larvae according to an embodiment of the present invention;
FIG. 4 is a graph of a persistence test for killing Aedes albopictus larvae according to an embodiment of the present invention;
FIG. 5 is a graph of a field test for killing Aedes albopictus larvae, according to an embodiment of the present invention;
Detailed Description
In order that those skilled in the art will better understand the present invention, the following technical scheme of the present invention will be further described with reference to the accompanying drawings and examples.
The invention relates to a mosquito-killing young slow-release formulation, which comprises the following active ingredients, wherein the first ingredient is biochar loaded with insecticide, and the second ingredient is three-dimensional grid gel formed by crosslinking sodium alginate and Ca2+;
the insecticidal composition is prepared by the following method:
s1: adding the biochar into deionized water according to a proportion, fully stirring to obtain a biochar suspension, then adding pesticide powder, and continuously stirring until the powder is completely dissolved and adsorbed to obtain the biochar suspension containing the pesticide, wherein the biochar suspension is used for preparing a slow-release dosage form subsequently;
s2: fully mixing biochar suspension containing insecticide with sodium alginate aqueous solution (stirring for not less than 12 hours), pouring into a mould, freezing and fixing for 1 hour at the temperature of minus 20 ℃, freezing into solid, pouring into a prepared calcium chloride aqueous solution, standing for 0.5 hour at room temperature to perform cross-linking reaction, filtering, washing and drying to form the black cylindrical microcapsule.
Further defined, in the first group, the insecticide moiety is one or more of the third generation nicotinic insecticide dinotefuran and the natural insecticide nocardianone.
Further defined, sodium alginate is prepared from sodium alginate powder and ultrapure water according to a ratio of 1:49, and the finished product has certain elasticity and gel strength after crosslinking in the proportion; the biochar suspension is prepared by mixing biochar powder and ultrapure water according to a ratio of 1:9, in this ratio, can make the drug-loading rate and release rate reach optimum ratio
Further defined, the content of biochar in the aqueous suspension of biochar is 1g/9mL; the mass fraction of sodium alginate in the sodium alginate aqueous solution is 2%; the mass fraction of CaCl2 in the calcium chloride solution is 2%.
Further defined, the mosquito-killing young slow release formulation is used for controlling aedes intermedium.
The slow released mosquito killing preparation is applied to various small and medium water bodies suitable for mosquito larva to grow.
Example 12 mg/cm3 dinotefuran-sodium alginate-biochar sustained release agent
The preparation method comprises the following steps: 4.00g of biochar powder is dissolved into 36ml of ultrapure water to prepare biochar suspension, 0.268g of dinotefuran powder is dissolved into the biochar suspension, 2.00g of sodium alginate is dissolved into 98ml of ultrapure water, the biochar suspension containing 0.268g of dinotefuran powder and the prepared aqueous solution of sodium alginate are mixed and stirred for 12 hours, poured into a cylindrical mold with the diameter of 0.8cm and the height of 1.1cm and frozen into solid, poured into a calcium chloride aqueous solution prepared by dissolving 4.00g of anhydrous calcium chloride into 196ml of ultrapure water to react for 0.5 hour, and filtered, washed with deionized water for 3 times and dried at 60 ℃ for 4 hours to prepare the 2mg/cm3 dinotefuran-sodium alginate-biochar slow release agent.
Example 2:3mg/cm3 dinotefuran-sodium alginate-biochar sustained release agent
The preparation method is carried out according to the preparation method of the example 1, and the 3mg/cm < 3 > dinotefuran-sodium alginate-biochar slow release agent is prepared.
7mg/cm3 dinotefuran-sodium alginate-biochar sustained release agent
Prepared according to the preparation method of the example 1 to prepare 7mg/cm3 dinotefuran-sodium alginate-biochar slow release agent.
Example 4:15mg/cm3 dinotefuran-sodium alginate-biochar slow release agent.
The preparation method is carried out according to the preparation method of the example 1, and the dinotefuran-sodium alginate-biochar slow release agent with the concentration of 15mg/cm < 3 > is prepared.
Application example 1: determination of release rate of sustained release agents with different drug loading rates in water body
In order to explore whether the dosage form can realize the slow release purpose after being put into a water body, the release speed of the medicine can be controlled according to different initial dosage proportions, and the dinotefuran content in the water body is measured by a high performance liquid chromatography method to judge. Under the conditions of 25 ℃ and 60% relative humidity, respectively testing the release conditions of the dinotefuran sustained release agent with two doses of 3mg/cm < 3 >, 7mg/cm < 3 >, firstly taking dinotefuran-sodium alginate-biochar sustained release agents with different drug loading amounts as an experimental group, taking a blank carrier as an experimental control group, soaking the experimental group in 200ml of ultrapure water, collecting water samples soaked for 1, 3, 5 and 7 days, and detecting the dinotefuran content in the water by using a high performance liquid chromatography;
the results are shown in figure 1, and the dosage form can control the release speed of the medicine and prolong the duration of the medicine. Meanwhile, as the drug loading rate is increased, the release rate of the drug is increased, the release rate is increased faster in the first three days, and the release rate is slowed down from the increase after the third day; the cumulative release rate on day 7 of the 7mg/cm3 group was about 15%, while the cumulative release rate on day 7 of the 3mg/cm3 group was about half the cumulative release rate of the 7mg/cm3 group, indicating that the cumulative release rate was related to drug loading, the greater the initial drug loading, the more drug was released per unit time.
Application example 2: determination of sustained release rate of sustained release agent at different temperatures
In order to explore whether the temperature has influence on the drug release of the dinotefuran sustained-release agent, the drug release condition of the 7mg/cm < 3 > dinotefuran sustained-release agent is tested under the conditions that the temperature is 15 ℃, the temperature is 25 ℃, the relative humidity is 35 ℃ and the relative humidity is 60%, and meanwhile, a carrier is set as an experimental control group, the dinotefuran sustained-release agent is soaked into 200ml of ultrapure water, and the dinotefuran content in water is detected by high performance liquid chromatography after water samples soaked for 1, 3, 5 and 7 days is collected.
The results are shown in fig. 2, and the dosage form can control the drug release rate. Meanwhile, the trend of the accumulated release rate of the medicine is consistent with the change of time at three temperatures, and the higher the temperature is, the faster the release rate of the active ingredients is, which indicates that the dosage form can accelerate the killing effect of mosquitoes through accelerating the release in the high-temperature season in which the mosquitoes are active.
Application example 3: quick-acting test for killing Aedes albopictus larvae
Test target: 3-year-last 4-year-old Aedes albopictus larva;
the test method comprises the following steps: adding 1 dinotefuran-sodium alginate-biochar slow release agent with one titer into each plastic bowl, adding 400ml of dechlorinated water, then adding 30 aedes albopictus larvae, feeding under laboratory conditions of 26+/-1 ℃ and 70+/-10% of relative humidity under natural illumination, recording the larva death number every 3 hours, and calculating the larva death rate for 24 hours and the shortest time required for killing all the larvae. The larvae were gently touched with a straw to avoid the stimulus as death.
Experimental control: 400ml of dechlorinated water and sodium alginate-biochar without dinotefuran powder are slowly released;
blank control: 400ml of dechlorinated water;
the result is shown in figure 3, the test result shows that the killing rate has dose dependency, the higher the initial drug carrying dosage is, the higher the killing rate is, the 24-hour killing rate of the 7mg/cm < 3 > dinotefuran-sodium alginate-biochar slow release agent is close to 100%, the 3-hour killing rate of the 15mg/cm < 3 > dinotefuran-sodium alginate-biochar slow release agent reaches 100%, and the 3-15 mg/cm < 3 > dinotefuran-sodium alginate-biochar slow release agent has good killing effect.
Application example 4: persistence test for killing Aedes albopictus larvae
Test target: 3-year-last 4-year-old Aedes albopictus larva;
the test method comprises the following steps: adding 1 dinotefuran-sodium alginate-biochar slow release agent with one titer into each plastic bowl for biological measurement, adding 400ml of dechlorinated water, performing one-time larva biological measurement at certain time intervals, adding 30 Aedes albopictus larvae into each plastic cup for biological measurement, feeding under laboratory conditions of 26+/-1 ℃ and relative humidity of 70+/-10% and natural illumination, and recording the larva killing rate for 24 hours. The larvae were gently touched with a straw to avoid the stimulus as death.
Blank vector control: 400ml of dechlorinated water and sodium alginate-biochar sustained release agent without insecticide;
blank control: 400ml of dechlorinated water;
the result is shown in figure 4, and the test result shows that the 3mg/cm3 dinotefuran-sodium alginate-biochar slow release agent has a larva mortality rate of more than 90% in each test before 110 days, a good killing effect, a long lasting period (110 days), and a killing rate of almost 100% in each test of the 7mg/cm3 dinotefuran-sodium alginate-biochar slow release agent, and has a good killing effect and a long lasting period (more than 160 days).
Application example 5: on-site test for killing Aedes albopictus larvae
Test target: 3-year-last 4-year-old Aedes albopictus larva;
the test method comprises the following steps: setting 6 experimental distribution points in the field habitat, placing 4 bowls of dechlorinated water in each distribution point, and adding 1 dinotefuran-sodium alginate-biochar slow release agent (3 mg/cm) 3 ) The bioassay evaluation is carried out once a week, the mouth of the container is covered by a 60-mesh gauze to prevent sundries such as leaves from falling into the bowl, the death condition of the test insects is recorded after 48 hours by each bioassay, the first time is carried out by adding dinotefuran-sodium alginate-biochar slow release agent for 1 day, and the repeated experiment period is 24 days. The larvae were gently touched with a straw to avoid the stimulus as death.
Blank control: 1L of dechlorinated water;
the results are shown in figure 5, and the multi-round results show that the duration of the effect is not less than 24 days, and the dinotefuran-sodium alginate-biochar slow release agent has better duration and killing effect overall.
The above embodiments are merely illustrative of the principles of the present invention and its effectiveness, and are not intended to limit the invention. Modifications and variations may be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the invention. Accordingly, it is intended that all equivalent modifications and variations of the invention be covered by the claims of this invention, which are within the skill of those skilled in the art, can be made without departing from the spirit and scope of the invention disclosed herein.
Claims (6)
1. A mosquito-killing young slow release formulation, which is characterized in that: the active ingredients of the composition comprise two components, wherein the first component is biochar loaded with insecticide, and the second component is three-dimensional grid gel formed by crosslinking sodium alginate and Ca2+;
the insecticidal composition is prepared by the following method:
s1: adding the biochar into deionized water according to a proportion, fully stirring to obtain a biochar suspension, then adding pesticide powder, and continuously stirring until the powder is completely dissolved and adsorbed to obtain the biochar suspension containing the pesticide, wherein the biochar suspension is used for preparing a slow-release dosage form subsequently;
s2: fully mixing biochar suspension containing insecticide with sodium alginate aqueous solution (stirring for not less than 12 hours), pouring into a mould, freezing and fixing for 1 hour at the temperature of minus 20 ℃, freezing into solid, pouring into a prepared calcium chloride aqueous solution, standing for 0.5 hour at room temperature to perform cross-linking reaction, filtering, washing and drying to form the black cylindrical microcapsule.
2. A mosquito-repellent child release dosage form according to claim 1, wherein: in the first group, the insecticide moiety is one or more of the third generation nicotinic insecticide dinotefuran and the natural insecticide nocardione.
3. A mosquito-repellent child release dosage form according to claim 2, wherein: the sodium alginate is prepared from sodium alginate powder and ultrapure water according to a proportion of 1:49, and the finished product has certain elasticity and gel strength after crosslinking in the proportion; the biochar suspension is prepared by mixing biochar powder and ultrapure water according to a ratio of 1:9, in this ratio, the drug loading and release rate can be optimized.
4. A mosquito-repellent child release dosage form according to claim 3, wherein: the content of the biochar in the aqueous suspension of the biochar is 1g/9mL; the mass fraction of sodium alginate in the sodium alginate aqueous solution is 2%; the mass fraction of CaCl2 in the calcium chloride solution is 2%.
5. The mosquito-repellent child release dosage form of claim 4, wherein: the mosquito killing young slow release formulation is used for preventing and controlling medium aedes.
6. Use of a mosquito-repellent young form of sustained release formulation according to any one of claims 1 to 5, characterized in that: is put into various small and medium-sized water bodies suitable for the growth of mosquito larvae.
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