Disclosure of Invention
In order to develop a composition for controlling oil from the source, which can be applied to hair products and optimizes the taste of the products, the first aspect of the present invention provides a plant fermentation product for controlling oil to be soothing and caring scalp, which is prepared from, by weight, 1-5 parts of cacumen Platycladi, 1-3 parts of licorice root, 1-3 parts of rose bud powder, 1-3 parts of rice, 1-2 parts of Ligusticum wallichii, 1-2 parts of ginseng root, 1-2 parts of wheat malt, 1-2 parts of ginger, 1-3 parts of Botrytis cinerea, 0.5-2 parts of fructo-oligosaccharides, and 70-80 parts of water.
As a preferred embodiment, the preparation raw materials comprise, by weight, 4 parts of cacumen biotae, 2 parts of licorice root, 2 parts of rose bud powder, 2 parts of rice, 1 part of ligusticum wallichii, 1 part of ginseng root, 1 part of wheat malt, 1 part of ginger, 2 parts of pteridophyte, 0.5-2 parts of fructo-oligosaccharide and 70-80 parts of water.
As a preferred embodiment, the preparation raw material further comprises a yeast combination and a lactobacillus group, wherein the addition amount of the yeast combination is 1-8% of the total mass of the preparation raw material, and the addition amount of the lactobacillus group is 2-7% of the total mass of the preparation raw material.
As a preferred embodiment, the yeast combination is added in an amount of 2-5% of the total mass of the preparation raw materials, and the lactobacillus group is added in an amount of 4-5% of the total mass of the preparation raw materials.
As a preferred embodiment, the yeast combination is added in an amount of 3% of the total mass of the preparation raw material, and the lactic acid bacteria group is added in an amount of 4.5% of the total mass of the preparation raw material.
As a preferred embodiment, the yeast combination comprises saccharomyces cerevisiae and kluyveromyces marxianus in a mass ratio of (1-5): 1.
as a preferred embodiment, the yeast combination comprises saccharomyces cerevisiae and kluyveromyces marxianus in a mass ratio of (2-4): 1.
as a preferred embodiment, the yeast combination comprises saccharomyces cerevisiae and kluyveromyces marxianus, wherein the mass ratio of the saccharomyces cerevisiae to the kluyveromyces marxianus is 3:1.
as a preferred embodiment, the concentration of the yeast combination is 10 6 -10 7 cfu/mL。
The applicant found during the experiment that by co-fermenting the plant composition with Saccharomyces cerevisiae and Kluyveromyces marxianus, the metabolites produced by decomposition of the plant composition have certain oil control and anti-drop effects, and have good care effect on the scalp, because the Saccharomyces cerevisiae and Kluyveromyces marxianus co-act and adopt 3:1 weight ratio, the two fungi can be cross decomposed and fermented to produce decomposition products with antiviral and antibacterial effects, which can relieve scalp health, diminish inflammation, remove dandruff, relieve itching and improve scalp ecological environment. The produced metabolites have synergistic increasing effect, can supplement nutrition components for scalp hair follicles, increase hair density and promote hair health.
As a preferred embodiment, the lactic acid bacteria group comprises one or a combination of several of lactobacillus casei, bifidobacterium adolescentis, lactobacillus plantarum, pediococcus pentosaceus, bifidobacterium longum and streptococcus thermophilus.
As a preferred embodiment, the lactobacillus group is a combination of lactobacillus casei, bifidobacterium adolescentis, lactobacillus plantarum and pediococcus pentosaceus, and the mass ratio is (1-3): (0.5-2): (1-3): (0.5-2).
As a preferred embodiment, the lactobacillus group is a combination of lactobacillus casei, bifidobacterium adolescentis, lactobacillus plantarum and pediococcus pentosaceus, and the mass ratio is (1.5-2.5): (1-1.5): (1.5-2.5): (1-1.5).
As a preferred embodiment, the lactobacillus group is a combination of lactobacillus casei, bifidobacterium adolescentis, lactobacillus plantarum and pediococcus pentosaceus, and the mass ratio is 2:1:2:1.
as a preferred embodiment, the concentration of the lactobacillus group is 10 6 -10 7 cfu/mL。
However, the applicant further found that the metabolic products produced after fermentation of saccharomyces cerevisiae and kluyveromyces marxianus are poor in stability and use effect in the combination process with the hair care product, the applicant can further cultivate the metabolic products by further introducing lactobacillus flora to improve the stability of the metabolic products, so that the metabolic products can exert good oil control effect in the hair care product, and particularly the lactobacillus flora of lactobacillus casei, bifidobacterium adolescentis and lactobacillus plantarum and pediococcus pentosaceus is adopted, so that the application effect is optimal, and the effect is probably that the metabolic products have a certain inhibition effect on the activity under the influence of the environment of the hair care product, so that the exertion of effective components is influenced, the tolerance of the metabolic products can be improved by further cultivation of the lactobacillus flora, and the obvious relaxing effect on the stimulation and the tightening sense of common surfactants is realized, so that the good oil control effect is volatilized.
In a second aspect, the present invention provides a method for preparing a plant fermentation product for oil control, soothing and scalp care, comprising the steps of:
s1, preparation of a plant culture medium: pulverizing folium Platycladi, glycyrrhrizae radix, flos Rosae Rugosae bud powder, rice, rhizoma Ligustici Chuanxiong, ginseng radix, wheat malt, rhizoma Zingiberis recens, and herba Pteridis Latifoliae, sieving, adding fructo-oligosaccharide and water, adjusting pH to 5.5-7.5, and sterilizing at 120-130deg.C for 10-30min to obtain plant culture medium;
s2, inoculating and fermenting in one step: inoculating yeast combination into plant culture medium, maintaining 30-35deg.C, sealing with gauze, shaking and fermenting at 120-200r/min for 8-16 hr, heating to 82-85deg.C, and sterilizing for 20-40min to obtain primary fermentation product;
s3, inoculating and fermenting in two steps: inoculating lactobacillus flora into a plant culture medium subjected to one-step inoculation fermentation, and culturing to obtain a secondary fermentation product;
s4, sterilization: sterilizing the secondary fermentation product at 60-80deg.C for 20-30min, and filtering with ceramic membrane; obtaining a plant fermentation product.
As a preferred embodiment, the method for preparing the plant fermentation product for controlling oil and soothing and caring scalp comprises the steps of:
s1, preparation of a plant culture medium: pulverizing folium Platycladi, glycyrrhrizae radix, flos Rosae Rugosae bud powder, rice, rhizoma Ligustici Chuanxiong, ginseng radix, wheat malt, rhizoma Zingiberis recens, and herba Pteridis Latifoliae, sieving, adding fructo-oligosaccharide and water, adjusting pH to 6-7, and sterilizing at 120-125deg.C for 10-30min to obtain plant culture medium;
s2, inoculating and fermenting in one step: inoculating yeast combination into plant culture medium, maintaining 30-32deg.C, sealing with gauze, shaking and fermenting at 150-200r/min for 10-15 hr, heating to 82-85deg.C, and sterilizing for 20-40min to obtain primary fermentation product;
s3, inoculating and fermenting in two steps: inoculating lactobacillus flora into a plant culture medium subjected to one-step inoculation fermentation, and culturing to obtain a secondary fermentation product;
s4, sterilization: sterilizing the secondary fermentation product at 60-80deg.C for 20-30min, and filtering with ceramic membrane; obtaining a plant fermentation product.
As a preferred embodiment, the crushed particle size in the step S1 is 40-100 mesh.
As a preferred embodiment, the culture conditions in step S3 are: culturing at 33-36 deg.C and 250-270rpm in a constant temperature culture shaker for 18-48 hr, and controlling pH to 4.5-6.5.
In a third aspect, the invention provides the use of a plant fermentation product for oil control, soothing and scalp care, in hair care products.
As a preferred embodiment, the hair care products include, but are not limited to, shampoos, hair care products, hair films, and essences.
Compared with the prior art, the invention has the following beneficial effects:
(1) According to the plant fermentation product for controlling oil and relieving and caring scalp, saccharomyces cerevisiae and kluyveromyces marxianus are fermented together for a series of plant combinations, so that metabolites generated by decomposing the plant compositions have certain oil control and good caring effects on scalp.
(2) The plant fermentation product for controlling oil and relieving and caring scalp can further cultivate metabolic products by further introducing lactobacillus flora, improves the stability of the metabolic products and enables the metabolic products to exert good oil control effect in hair care products.
(3) The plant fermentation product for controlling oil and relieving and caring scalp comprises the composition of plant and probiotics fermentation, which is natural plant and biological strain, has good antibacterial effect, and the components are synergistic and green and safe, and can effectively remove dandruff and oil through scientific proportion, thereby improving scalp ecology; by repairing scalp barrier, the scalp sebum secretion is controlled, the effects of relieving inflammation, removing dandruff and relieving itching are achieved, and the scalp health state is helped to be recovered. Meanwhile, the hair density can be obviously increased, the hair quality is promoted to be healthy, and the hair is softened.
(4) The plant fermentation product for controlling oil and relieving and caring scalp has obvious relieving effect on the stimulation and tightening sense of common surfactants in the hair washing process, and has unique fermentation smell and pleasant smell.
Detailed Description
The classification of Saccharomyces cerevisiae in the embodiment is named as Saccharomyces cerevisiae Saccharomyces cerevisiae which is preserved in China general microbiological culture Collection center, beijing, which is the address of China, and is preserved in the 28 th year of 2023, and the preservation label is CGMCC No.28276.
Example 1
The plant fermentation product for controlling oil and relieving and caring scalp comprises, by weight, 4 parts of biota orientalis leaves, 2 parts of licorice roots, 2 parts of rose bud powder, 2 parts of rice, 1 part of ligusticum wallichii, 1 part of ginseng roots, 1 part of wheat malt, 1 part of ginger, 2 parts of fern, 1 part of fructo-oligosaccharides and 75 parts of water.
The preparation raw materials also comprise yeast combinations and lactobacillus groups, wherein the addition amount of the yeast combinations is 3% of the total mass of the preparation raw materials, and the addition amount of the lactobacillus groups is 4.5% of the total mass of the preparation raw materials.
The yeast combination comprises saccharomyces cerevisiae and kluyveromyces marxianus, wherein the mass ratio of the saccharomyces cerevisiae to the kluyveromyces marxianus is 3:1. the concentration of the yeast combination was 10 7 cfu/mL。
The lactobacillus group is the combination of lactobacillus casei, bifidobacterium adolescentis, lactobacillus plantarum and pediococcus pentosaceus, and the mass ratio is 2:1:2:1. the concentration of the lactobacillus flora is 10 7 cfu/mL。
A method for preparing a plant fermentation product for controlling oil and soothing and caring scalp, comprising the following steps:
s1, preparation of a plant culture medium: pulverizing folium Platycladi, glycyrrhrizae radix, flos Rosae Rugosae bud powder, rice, rhizoma Ligustici Chuanxiong, ginseng radix, wheat malt, rhizoma Zingiberis recens, and herba Pteridis Latifoliae, sieving with 80 mesh sieve, adding fructo-oligosaccharide and water, adjusting pH to 6.5, and sterilizing at 121deg.C for 20min to obtain plant culture medium;
s2, inoculating and fermenting in one step: inoculating yeast combination into a plant culture medium, keeping 31 ℃, sealing with gauze, shaking and fermenting at 180r/min for 13h, heating to 83 ℃ and sterilizing for 30min to obtain a primary fermentation product;
s3, inoculating and fermenting in two steps: inoculating lactobacillus flora into a plant culture medium subjected to one-step inoculation fermentation, culturing at 35 ℃ in a constant-temperature culture shaking table at 260rpm for 33 hours, and controlling pH to 5.5 to obtain a secondary fermentation product;
s4, sterilization: sterilizing the secondary fermentation product at 70deg.C for 25min, and filtering with ceramic membrane; obtaining a plant fermentation product.
Example 2
The plant fermentation product for controlling oil and relieving and caring scalp comprises, by weight, 1 part of biota orientalis, 1 part of licorice root, 1 part of rose bud powder, 1 part of rice, 1 part of Ligusticum wallichii, 1 part of ginseng root, 1 part of wheat malt, 1 part of ginger, 1 part of fern, 0.5 part of fructo-oligosaccharide and 70 parts of water.
The preparation raw materials also comprise yeast combinations and lactobacillus groups, wherein the addition amount of the yeast combinations is 2% of the total mass of the preparation raw materials, and the addition amount of the lactobacillus groups is 4% of the total mass of the preparation raw materials.
The yeast combination comprises saccharomyces cerevisiae and kluyveromyces marxianus, wherein the mass ratio of the saccharomyces cerevisiae to the kluyveromyces marxianus is 2:1. the concentration of the yeast combination was 10 6 cfu/mL。
The lactobacillus group is the combination of lactobacillus casei, bifidobacterium adolescentis, lactobacillus plantarum and pediococcus pentosaceus, and the mass ratio is 1.5:1:1.5:1. the concentration of the lactobacillus flora is 10 6 cfu/mL。
A method for preparing a plant fermentation product for controlling oil and soothing and caring scalp, comprising the following steps:
s1, preparation of a plant culture medium: pulverizing folium Platycladi, glycyrrhrizae radix, flos Rosae Rugosae bud powder, rice, rhizoma Ligustici Chuanxiong, ginseng radix, wheat malt, rhizoma Zingiberis recens, and herba Pteridis Latifoliae, sieving with 50 mesh sieve, adding fructo-oligosaccharide and water, adjusting pH to 6, sterilizing at 120deg.C for 30min to obtain plant culture medium;
s2, inoculating and fermenting in one step: inoculating yeast combination into a plant culture medium, maintaining 30 ℃, sealing with gauze, shaking and fermenting at 200r/min for 15h, heating to 82 ℃ and sterilizing for 40min to obtain a primary fermentation product;
s3, inoculating and fermenting in two steps: inoculating lactobacillus flora into a plant culture medium subjected to one-step inoculation fermentation, culturing at 33 ℃ in a constant-temperature culture shaker at 250rpm for 48 hours, and controlling pH to 4.5 to obtain a secondary fermentation product;
s4, sterilization: sterilizing the secondary fermentation product at 60 ℃ for 30min, and filtering by a ceramic membrane; obtaining a plant fermentation product.
Example 3
The plant fermentation product for controlling oil and relieving and caring scalp comprises, by weight, 5 parts of biota orientalis leaves, 3 parts of licorice roots, 3 parts of rose bud powder, 3 parts of rice, 2 parts of ligusticum wallichii, 2 parts of ginseng roots, 2 parts of wheat malt, 2 parts of ginger, 3 parts of fern, 2 parts of fructo-oligosaccharides and 80 parts of water.
The preparation raw materials also comprise yeast combinations and lactobacillus groups, wherein the addition amount of the yeast combinations is 4% of the total mass of the preparation raw materials, and the addition amount of the lactobacillus groups is 5% of the total mass of the preparation raw materials.
The yeast combination comprises saccharomyces cerevisiae and kluyveromyces marxianus, wherein the mass ratio of the saccharomyces cerevisiae to the kluyveromyces marxianus is 4:1. the concentration of the yeast combination was 10 7 cfu/mL。
The lactobacillus group is the combination of lactobacillus casei, bifidobacterium adolescentis, lactobacillus plantarum and pediococcus pentosaceus, and the mass ratio is 2.5:1.5:2.5:1.5. the concentration of the lactobacillus flora is 10 6 cfu/mL。
A method for preparing a plant fermentation product for controlling oil and soothing and caring scalp, comprising the following steps:
s1, preparation of a plant culture medium: pulverizing folium Platycladi, glycyrrhrizae radix, flos Rosae Rugosae bud powder, rice, rhizoma Ligustici Chuanxiong, ginseng radix, wheat malt, rhizoma Zingiberis recens, and herba Pteridis Latifoliae, sieving with 100 mesh sieve, adding fructo-oligosaccharide and water, adjusting pH to 7, and sterilizing at 125deg.C for 10min to obtain plant culture medium;
s2, inoculating and fermenting in one step: inoculating yeast combination into a plant culture medium, maintaining 32 ℃, sealing with gauze, shaking and fermenting at 150r/min for 10h, heating to 85 ℃ and sterilizing for 20min to obtain a primary fermentation product;
s3, inoculating and fermenting in two steps: inoculating lactobacillus flora into a plant culture medium subjected to one-step inoculation fermentation, culturing at 36 ℃ in a constant-temperature culture shaker at 250rpm for 18 hours, and controlling pH to 6.5 to obtain a secondary fermentation product;
s4, sterilization: sterilizing the secondary fermentation product at 80deg.C for 20min, and filtering with ceramic membrane; obtaining a plant fermentation product.
Example 4
A plant fermentation product for oil management and scalp care, the specific procedure being as in example 1, except that the yeast combination comprises only Saccharomyces cerevisiae.
Example 5
The specific steps of the plant fermentation product for controlling oil and relieving and caring scalp are the same as those of the example 1, wherein the mass ratio of the saccharomyces cerevisiae to the kluyveromyces marxianus is 5:1.
example 6
The specific steps of the plant fermentation product for controlling oil and relieving and caring scalp are the same as those of the example 1, and the difference is that the lactobacillus flora is a combination of lactobacillus casei, bifidobacterium longum, lactobacillus plantarum and streptococcus thermophilus, and the mass ratio is 2:1:2:1.
example 7
The specific steps of the plant fermentation product for controlling oil and relieving and caring scalp are the same as those of the example 1, wherein the weight ratio of lactobacillus casei, bifidobacterium longum, lactobacillus plantarum and streptococcus thermophilus is 3:2:3:2.
performance testing
1. Inflammatory factor inhibition assay
The experimental method comprises the following steps: RAW264.7 macrophages were used as subjects to establish a model of cellular inflammation by stimulating cells with lipopolysaccharide LPS (bacterial endotoxin). Macrophage cells were inoculated into 12-well plates at 37℃in an incubator with 5% CO 2 Incubating 24h under aeration conditions, adding 1wt% dilutions of the plant fermentation products of examples 1-7, respectively, adding LPS (1 μg/mL) after 2h, and setting a group to which no dilutions were added and a group to which no LPS was added, stimulating for 24 hours, collecting the supernatant, centrifuging, and detecting. The level of proinflammatory inflammatory factor TNF-alpha release from RAW264.7 was analyzed using ELISA kit.
TNF- α inhibition was calculated from the following formula:
TNF-alpha inhibition ratio (%) = (LPS-stimulated inflammatory factor concentration-test agent-active inflammatory factor concentration)/(LPS-stimulated inflammatory factor concentration-non-stimulated inflammatory factor concentration) ×100%. The higher the TNF-alpha inhibition, the better the anti-inflammatory effect of the multi-strain fermentation filtrate.
The TNF- α inhibition test results are shown in Table 1.
TABLE 1
2. Oil control efficacy test
(1) Preparation of the reagent:
testosterone (T) solution: 36 mg testosterone is dissolved in absolute ethyl alcohol to prepare testosterone solution with the concentration of 5 mmol/L;
NADPH (reduced coenzyme ii) solution: dissolving 42.5 mg of NADPH tetrasodium salt in water to prepare an NADPH solution with the concentration of 2 mmol/L;
the reaction solution: DTT (dithiothreitol) and sodium phosphate were dissolved together in water so that the final concentrations of DTT and sodium phosphate were 1 mmol/L and 20 mmo/L, respectively, to obtain a reaction solution.
(2) Determination of 5α -reductase Activity:
the reaction components were mixed in the order of succession (155. Mu.L of reaction solution, constant temperature at 37 ℃, 10. Mu.L of testosterone solution, 10. Mu.L of NADPH solution, and 20. Mu.L of 5α -reductase), and the change in absorbance of the reaction system at 340 and nm over 4 minutes was continuously monitored to reflect the activity of 5α -reductase at a rate of change (the amount of enzyme whose NADPH concentration was decreased by 1. Mu. Mol/L per minute in the reaction system at 37 ℃ C. Was one enzyme activity unit), and the reaction system was recorded as the initial enzyme activity.
(3) Determination of 5. Alpha. -reductase inhibition ratio of the fermented product:
the reaction components were mixed in the order of succession (155. Mu.L of reaction solution, constant temperature of 37 ℃, 10. Mu.L of testosterone solution, 10. Mu.L of NADPH solution, 15. Mu.L of plant fermentation products of examples 1 to 7, 20. Mu.L of 5α -reductase, respectively), the change of absorbance value of the reaction system at 340 and nm over 4 minutes was continuously monitored, the enzyme activity of 5α -reductase inactivation was calculated with reference to an NADPH standard curve, and the 5α -reductase inhibition rate of the fermentation complex was calculated based on "5α -reductase inhibition rate=inactivated enzyme activity/initial enzyme activity×100%". The test results are shown in Table 2.
TABLE 2
3. Antibacterial effect (antibacterial circle)
The strain liquid is selected from the following steps: staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, malassezia furfur; preparation: diluting with sterile physiological saline to about 1.0x10 5 cfu/ml~1.0x10 6 cfu/ml of bacterial suspension. The prepared bacterial suspension should be used within 2 hours or stored at 4 ℃ for not more than 24 hours.
And (3) antibacterial effect detection: the plant fermentation products obtained in examples 1 to 7 were collected, and sterile dried filter paper sheets were each dropped with 20. Mu.L of an aqueous composition solution (20 mg/mL) at the actual use concentration. Inoculating test bacteria: taking 1mL of each test bacterial suspension, and uniformly coating the suspension on the surface of a nutrient agar culture medium; the antibacterial test sample is pasted, a bacteria-staining flat plate is pasted for each test, the filter paper sheets are 4 sheets per dish, 3 sheets are test sample sheets, and 1 sheet is a control sample sheet and is blank. The bacteria-free tweezers are used for taking a sample, the sample is attached to the surface of a flat plate, the flat plate is placed in a constant temperature incubator at 36+/-1 ℃, the result is observed after the culture is carried out for 18 hours, and the diameter of the bacteria inhibition ring is measured by a vernier caliper. The results are the average of 3 measurements. The test results are shown in Table 3.
TABLE 3 Table 3
4. Application test: the plant fermentation products prepared in examples 1 to 7 were formulated into an essential milk according to table 4 and tested.
TABLE 4 Table 4
4.1 scalp oil control efficacy test
(1) Scalp oil control efficacy test
Selecting a subject with a greasy scalp state, wherein the age is between 20 and 45 years, the subject voluntarily participates in the test and signs an informed consent, and the subject prohibits the use of any hair product except the specified shampoo (the commercially available shampoo without oil control effect) and the test essence during the test. The temperature of the test environment is 23-25 ℃ and the humidity is 40-60%.
The condition of the testers was investigated in advance and tested in groups, and the initial value of scalp fat content was tested using an Antsci Skin tester (Skin fat test probe) without washing hair 24 hours before the test of the subjects. After the specified commercial shampoos were used on site, the hair was dried, and each group was respectively smeared with the essence emulsions prepared in examples 1 to 7 of the present application, and after 10 minutes of massage, the scalp was immediately tested for oil content by an Antsci Skin-SP Skin tester (Skin oil test probe) without washing with water, and recorded as data of 0 h. And sitting still for 4 hours and 8 hours in the test environment, and measuring the scalp grease content of the same area of the scalp of the subject. The results were averaged. The test results are shown in Table 5.
TABLE 5
4.2 anti-hair loss efficacy test:
selecting healthy men or women with ages of 25-50 years old; has the problems of more alopecia or sparse hair; volunteers who did not undergo hair treatment such as hair dyeing, hair waving, etc. for nearly 1 month participate in the test. 3 areas with an area of 1.5cm x 1.5cm, which are at least 5cm away from each other, are selected from the head as test areas, and a negative control (blank matrix), a positive control (vindimidiate tincture 2 wt%) and the essence milk test sample prepared in example 1 are randomly used, and the test is carried out on the test samples before the test samples are used by the test subjects, after the test subjects use the test products for 4 weeks and 8 weeks, and the test subjects are controlled in a constant temperature and constant humidity environment at a temperature of 22+/-1 ℃ and a humidity of 50+/-10% RH. In the corresponding test areas, the evaluation is performed in the following order:
1) Cutting hair in 3 test areas to within 5mm and bringing the hair as close as possible to the scalp;
2) Imaging the 3 test areas with a scalp analyzer, respectively;
3) About 48 hours apart;
4) The 3 test areas were imaged separately with a scalp analyzer.
After the end of the entire test program, the hair density and hair growth rate were analyzed.
Hair density: the scalp and hair of the test area were imaged by a korean KC company scalp analyzer, and the number of hairs in the photograph was counted. Meanwhile, the area of the scalp region photographed in the photograph is calculated according to the scale of the photograph. According to the formula d=n/S, where N is the number of hairs in the photograph in root; s is the photo area in cm 2 The method comprises the steps of carrying out a first treatment on the surface of the D is hair density in root/cm 2 . The larger the value of the hair density D, the more hair per unit area, the denser the hair.
Hair density: δd=d Tn −D T0
Normalized hair density = DTn/DT 0x 100%
Wherein D is T0 Hair density before application of the product to the test area; d (D) Tn Hair density after application of the product to the test area.
Hair growth rate: the hair of the test area was cut to within 5mm at intervals of 2 days through korea, respectivelyThe KC company scalp analyzer images scalp and hair in the test area, and the positions and angles of the two images are kept consistent, so that each hair in the two images can be in one-to-one correspondence. The length of each hair in the photograph was calculated from the scale of the photograph. According to the formula r= (L 2 -L 1 ) 2 calculating the hair growth rate, wherein L 1 The length of hair in mm in the photograph at the time of imaging 1 st; l (L) 2 The length of the same hair after 2 days interval is in mm; r is the hair growth rate in mm/day. The larger the value of the hair growth rate R, the faster the hair growth.
The results of the hair density and hair growth rate tests are shown in Table 6.
TABLE 6
Test results show that the oil control and refined emulsion can increase hair density, promote hair growth and has a certain alopecia prevention effect.
4.3 Patch test
Materials and methods
1. Test article: example 1 essence emulsion
2. Negative control: blank control
3. The subject: a total of 30, men 12, women 18, ages 18-59, subjects meeting the following criteria were excluded:
(1) Women in lactation or gestation period
(2) Highly sensitive body constitution
(3) Antihistamines used in the last week or immunosuppressants used in the last month
(4) Any anti-inflammatory drug is administered to the subject at the site of approximately two months
(5) Has inflammatory dermatosis and does not heal
(6) Insulin dependent diabetes mellitus patient
(7) Patients suffering from asthma or other chronic respiratory diseases undergoing treatment
(8) Patients who receive cancer chemotherapy within six months
(9) Patients with immunodeficiency or autoimmune diseases
(10) Bilateral mastectomy and bilateral axillary lymphadenectomy
(11) Researchers taking part in other clinical trials
(12) The test area has skin characterization with large area of mole, scratch, white spot, nevus pigmentosus, keloid, etc. affecting test
(13) Non-volunteer participants or those who were unable to complete the prescribed content as required by the experiment.
4. The test method comprises the following steps: selecting a qualified patch tester, adding 0.020g-0.025g of a test object into the patch tester by a closed patch test method, applying a special adhesive tape to the back of a subject, removing the test object after 24 hours, observing skin reactions after 0.5, 24 and 48 hours respectively, and recording the results according to the skin grading standard in cosmetic safety technical Specification (2015).
Skin adverse reaction grading criteria.
The test results are shown in Table 7.
TABLE 7
The test results of the human skin patch show that adverse reactions occur in 0 cases among 30 people.