CN117467575B - Brucella lupekinensis K6 with salt-tolerant growth-promoting function and application thereof - Google Patents
Brucella lupekinensis K6 with salt-tolerant growth-promoting function and application thereof Download PDFInfo
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- 241000589562 Brucella Species 0.000 title claims abstract description 60
- 239000002689 soil Substances 0.000 claims abstract description 55
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000003513 alkali Substances 0.000 claims abstract description 34
- 239000011574 phosphorus Substances 0.000 claims abstract description 34
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 34
- 239000001963 growth medium Substances 0.000 claims abstract description 31
- 230000012010 growth Effects 0.000 claims abstract description 29
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000011591 potassium Substances 0.000 claims abstract description 26
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 26
- 230000015784 hyperosmotic salinity response Effects 0.000 claims abstract description 21
- 238000009630 liquid culture Methods 0.000 claims abstract description 14
- 102100028717 Cytosolic 5'-nucleotidase 3A Human genes 0.000 claims abstract description 9
- 241000219745 Lupinus Species 0.000 claims abstract description 9
- 238000000855 fermentation Methods 0.000 claims abstract description 9
- 230000004151 fermentation Effects 0.000 claims abstract description 9
- 238000009629 microbiological culture Methods 0.000 claims abstract description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 25
- 150000003839 salts Chemical class 0.000 claims description 25
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 16
- 239000000843 powder Substances 0.000 claims description 15
- 239000011780 sodium chloride Substances 0.000 claims description 13
- 239000000725 suspension Substances 0.000 claims description 11
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 10
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- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 8
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 8
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- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 4
- 235000011010 calcium phosphates Nutrition 0.000 claims description 4
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 4
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- 108020004465 16S ribosomal RNA Proteins 0.000 description 12
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- 108090000623 proteins and genes Proteins 0.000 description 11
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- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 5
- 235000011130 ammonium sulphate Nutrition 0.000 description 5
- 229940099596 manganese sulfate Drugs 0.000 description 5
- 239000011702 manganese sulphate Substances 0.000 description 5
- 235000007079 manganese sulphate Nutrition 0.000 description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 5
- 230000001105 regulatory effect Effects 0.000 description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 4
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- 235000015097 nutrients Nutrition 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000003794 Gram staining Methods 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 3
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- 241000894006 Bacteria Species 0.000 description 2
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
- DLHONNLASJQAHX-UHFFFAOYSA-N aluminum;potassium;oxygen(2-);silicon(4+) Chemical compound [O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[Al+3].[Si+4].[Si+4].[Si+4].[K+] DLHONNLASJQAHX-UHFFFAOYSA-N 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
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- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
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- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000586290 Suaeda salsa Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
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- 239000002054 inoculum Substances 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
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- 230000000877 morphologic effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- -1 salt ions Chemical class 0.000 description 1
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- 239000008223 sterile water Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention relates to a strain of brucella lupeus, in particular to a strain of brucella lupeus K6 with salt tolerance and growth promotion functions and application thereof, and belongs to the technical field of agricultural microorganisms. The preservation number of the lupin brucella K6 is CGMCC No.27796, and the lupin brucella K6 is preserved in the China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) at 7 and 4 of 2023. The strain obtained by the invention can improve the contents of quick-acting potassium and available phosphorus in soil under high-salt stress; the invention also provides a liquid culture medium suitable for fermentation of the lupin brucella K6, and the lupin brucella K6 with salt-tolerant growth-promoting function can be rapidly obtained in large quantity by using the culture medium, and the salt-tolerant growth-promoting strain provides excellent strain resources for restoring saline-alkali farmland soil, and has higher agricultural application prospect and economic value.
Description
Technical Field
The invention relates to a strain of brucella lupeus, in particular to a strain of brucella lupeus K6 with salt tolerance and growth promotion functions and application thereof, and belongs to the technical field of agricultural microorganisms.
Background
The salt-tolerant microorganism has a special salt-tolerant mechanism, can maintain normal growth metabolism in a high-salt environment, and is a novel microorganism resource. The 5 hundred million mu of saline-alkali soil in China has urgent development and utilization potential, and is an important potential cultivated land resource. The growth promoting function of the functional microorganisms with salt tolerance can effectively improve the saline-alkali soil and the crop yield in the saline-alkali farmland. Therefore, the breeding of efficient and practical soil functional microorganism resources has important significance.
Under the stress of salt and alkali, the microbial quantity in the soil is obviously reduced, and the microbial community structure, the diversity, the community function and the like are changed. The saline-alkali soil has high-salt and barren special habitat, and contains a plurality of rich salt-tolerant microbial resources, in particular to plant growth-promoting strains with salt tolerance. By excavating microorganisms with salt-tolerant growth-promoting functions in the soil of the saline-alkali soil and utilizing the growth-promoting functions of the microorganisms, the plant flora environment can be effectively improved, the effective nutrient elements in the soil can be increased, the improvement and repair of the saline-alkali soil can be accelerated, the crop habitat of the saline-alkali soil can be optimized, and the soil utilization rate of the saline-alkali soil can be improved.
The rich and diverse salt-tolerant growth-promoting flora can convert the nutrient elements which are difficult to be utilized in the soil into quick-acting or soluble nutrient substances, provide the plant with nutrient elements which are easy to absorb and utilize, and can effectively relieve the toxic action of salt ions in the environment on the plant. However, the research on salt-tolerant growth-promoting strains in the prior art is less, and strain resources applicable to actual production are still relatively poor. The high-salt-tolerance culturable microorganism with multiple growth promoting functions such as potassium dissolving, phosphorus dissolving, nitrogen fixing and the like can survive in a high-salinity environment, and the brucella lupeus capable of effectively improving the utilization rate of the saline-alkali soil and the application of the brucella lupeus in increasing the quick-acting potassium and the effective phosphorus of the saline-alkali soil are not reported.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the brucella lupeus K6 with the salt-tolerant growth-promoting function.
The invention also aims to provide the application of the brucella lupeus K6 in increasing the quick-acting potassium and the effective phosphorus of the saline-alkali soil.
The technical scheme adopted by the invention for achieving the purpose is as follows:
The invention provides a lupeum album strain K6 with salt tolerance and growth promotion functions, wherein the preservation number of the lupeum album strain K6 is CGMCC No. 27796, and the lupeum strain K6 is preserved in the China general microbiological culture Collection center of China general microbiological culture Collection center (China general microbiological culture Collection center) for 7 th month 4 of 2023.
The invention also provides an application of the lupeum lupulum K6 with the salt tolerance and growth promotion functions in increasing quick-acting potassium and effective phosphorus in saline-alkali soil under the condition of salt stress.
Further, in the application process, the salt stress refers to saline-alkali soil with the mass ratio of the salt concentration in the soil not lower than 3 per mill.
The invention provides an application process of increasing quick-acting potassium and effective phosphorus in saline-alkali soil under salt stress of lupeus K6 with salt-tolerant growth-promoting function, which specifically comprises the following steps:
(1) Inoculating the activated brucella lupeus K6 seed solution into a fermentation liquid culture medium for culturing to obtain a brucella lupeus-containing suspension;
(2) Diluting the fermented brucella lupeus suspension, and applying the diluted brucella lupeus suspension into saline-alkali soil.
In the step (1), the inoculation is carried out by inoculating the activated brucella lupeus seed liquid according to the volume ratio of 1-2%.
In the step (1), the fermentation liquid culture medium contains 10 g g of glucose, 2g of yeast powder, 10 g g of sodium chloride, 0.5 g g of potassium chloride, 3 g g of calcium phosphate, 0.05g of ferrous sulfate, 0.2g of magnesium sulfate and the pH value is adjusted to 7.5-8.0 in tap water per 1000 mL.
Further, in the step (1), the culture condition is that the culture is carried out for 24-48 hours under the conditions of 28-30 ℃ and 180 rpm.
Further, the concentration of the diluted Brucella lupekoe suspension is 1X 10 6cfu/mL~1×1010 cfu/mL.
The lupeus brucella K6 with salt tolerance and growth promotion functions obtained by screening is characterized in that: the cell morphology is circular or oval, no spores are generated, and the cell size is (0.2 μm to 0.5 μm) x (1.2 μm to 1.5 μm). The colony is round, smaller, light yellow, opaque, moist in surface, easy to pick and irregular in edge when in solid culture at 28 ℃; the physiological and biochemical characteristics are as follows: gram staining is negative, aerobic, the optimal growth temperature is 25-33 ℃, the optimal growth pH value is 6.5-8.0, and the optimal salt concentration is 1-5%, so that the method has the characteristics of potassium dissolving, phosphorus dissolving and nitrogen fixing.
The result of measuring the 16S rRNA gene sequence of the brucella lupeus K6 shows that the gene length is 1392 bp, and the corresponding nucleotide sequence is shown as SEQ ID NO. 1.
The strain K6 of the present invention was determined to be highly homologous to the model strain Brucella lupeus (Brucella lupini) NBRC 102587 with sequence similarity as high as 99.87% by using BLASTN program alignment and phylogenetic analysis at the American bioengineering information center (National Center for Biotechnology Information, NCBI). The 16S rDNA sequence of 15 sequences with higher homology is selected as a reference object, and a phylogenetic tree between the strain K6 and a reference strain is constructed by using Mega 7 software by adopting a proximity method (neighbor-Joining). In the evolutionary tree, strain K6 forms a separate intra-cluster evolutionary branch with Brucella lupeus (Brucella lupini) NBRC 102587 (FIG. 7). Thus, K6 was determined to be a strain of Brucella lupekoe (Brucella lupini).
The basic method for breeding the lupeum lupulus K6 strain with the salt-tolerant growth-promoting function comprises the following steps:
Collecting saline-alkali soil at the saline-alkali soil in the natural protection area of yellow river delta, placing 1g of saline-alkali soil in a potassium-dissolving liquid culture medium, carrying out enrichment culture for 48 hours in a 180 rpm constant-temperature shaking incubator at 28-30 ℃, carrying out gradient dilution and coating on a potassium-dissolving solid culture plate, carrying out stationary culture at 28-30 ℃, picking single colony on the same culture plate, purifying twice to obtain a strain, and carrying out glycerol freezing preservation and bacteria preservation.
The invention relates to application of lupeum lupulum K6 with salt tolerance and growth promotion functions in increasing the content of quick-acting potassium in soil, wherein the content of quick-acting potassium in soil is increased by 43.7%. The invention relates to application of lupeum lupulum K6 with salt tolerance and growth promotion functions in increasing the content of available phosphorus in soil, wherein the content of the available phosphorus in the soil is increased by 32.9%.
The beneficial effects of the invention are as follows:
(1) The invention discloses a lupeus strain K6 with salt tolerance and growth promotion functions, which has the characteristics of high salt tolerance, potassium dissolution, phosphorus dissolution and the like, has a wide salt tolerance range, can keep good growth vigor under the condition of 1-5% salt concentration, and has great potential for being applied to saline-alkali soil restoration and agricultural production.
(2) According to the invention, through multiple rounds of screening, a salt-tolerant strain with multiple growth promoting functions is obtained, and under high salt stress, the content of quick-acting potassium and effective phosphorus in soil can be obviously increased by 43.7% and 32.9% respectively; the invention provides the lupin brucella K6 and a liquid culture medium suitable for fermentation of the lupin brucella K6, and the lupin brucella K6 with salt-tolerant growth-promoting function can be rapidly obtained in large quantity by using the culture medium.
Preservation information
Preservation time: 2023, 07, 04;
Preservation unit: china general microbiological culture Collection center (China Committee for culture Collection);
Preservation number: CGMCC No.27796;
deposit unit address: the institute of microorganisms at national academy of sciences of China, national academy of sciences, no. 1, north Star West way, no. 3, chat.Chao, beijing, city;
Postal code: 100101;
classification naming: brucella lupescens Brucella lupini.
Drawings
FIG. 1 shows the cell morphology of B.lupeum K6 under a microscope;
FIG. 2 shows colony morphology of Brucella lupeum K6;
FIG. 3 shows the growth curves of Brucella lupeum K6 under different salt concentrations;
FIG. 4 is a graph showing the potassium-solubilizing effect of Brucella lupeum K6;
FIG. 5 is a graph showing the effect of P.lupeum K6 on phosphorus dissolution (organophosphorus);
FIG. 6 is a graph showing the effect of P.lupeum K6 on phosphorus dissolution (inorganic phosphorus);
FIG. 7 is a phylogenetic tree between Brucella lupeonii K6 and a reference strain.
Detailed Description
The present invention will be described in detail with reference to the following drawings and examples. The following examples are only preferred embodiments of the present invention, and it should be noted that the following descriptions are merely for explaining the present invention, and are not limiting in any way, and any simple modification, equivalent variation and modification of the embodiments according to the technical principles of the present invention are within the scope of the technical solutions of the present invention.
In the following examples, materials, reagents and the like used, unless otherwise specified, were obtained commercially.
EXAMPLE 1 screening of salt tolerant Brucella lupekoe
(1) Collecting root soil sample of Suaeda salsa from saline-alkali soil in natural protection area of yellow river delta, filling into clean sampling bag, marking, placing into ice box, taking back to laboratory, and preserving at-20deg.C for use. 1g soil samples are weighed in a sterile super clean bench and placed in triangular flasks containing 20 mL potassium-dissolving screening liquid culture medium, and enrichment culture is carried out for 48 hours in a 180 rpm shaking incubator at the temperature of 28 ℃. Under the aseptic condition, respectively sucking 1 mL culture solution into 9 mL sterile water, fully mixing, then sequentially carrying out gradient dilution to prepare 10 -1、10-3、10-5 sample solutions with different dilutions, respectively sucking 0.2 mL sample solutions, coating the sample solutions on a potassium-dissolving solid culture medium flat plate, carrying out inverted culture in a constant temperature incubator at 28 ℃ for 2-5 days, and observing whether oil drop-shaped colonies are generated on the flat plate. And (3) selecting single colonies on the degrading ring, transferring the single colonies to the same solid culture medium plate, numbering the single colonies in sequence, culturing the single colonies, and transferring and streaking the single colonies twice to obtain the pure culture strain.
(2) Single colonies are picked by an inoculating loop, transferred into a test tube filled with 5mL salt-tolerant screening liquid culture medium, and subjected to shaking culture at 28 ℃ and 180 rpm for 24 h, and strain preservation and physiological and biochemical analysis respectively. The strain preservation method adopts a glycerol tube freezing preservation method, 200 mu L of glycerol (the final concentration of the glycerol is 20%) and 800 mu L of bacterial liquid are respectively added into the freezing preservation tube, and the mixture is placed in an ultralow temperature refrigerator at-80 ℃ for preservation after uniform mixing.
In the screening of the strain, one strain of the strain has a circular or oval shape, the cell size (0.2 μm to 0.5 μm) x (1.2 μm to 1.5 μm) and no spores are generated. The colony is round, smaller, light yellow, opaque, smooth and moist in surface, easy to pick and irregular in edge when in solid culture at 28 ℃; the physiological and biochemical characteristics are as follows: gram staining is negative, aerobic, the optimal growth temperature is 25-33 ℃, the optimal growth pH value is 6.5-8.0, the optimal salt concentration is 1-5%, the salt tolerance range is 1-7%, and the composite material has potassium dissolving, phosphorus dissolving and nitrogen fixing characteristics. The strain number was K6, which was initially determined as the selected strain K6 of the present invention.
The composition of the salt-resistant screening solid culture medium is as follows: every 1000 mL distilled water contains glucose 5 g, yeast extract 0.5 g, disodium hydrogen phosphate 2.0 g, ammonium sulfate 0.5 g, manganese sulfate 0.03 g, ferrous sulfate 0.03 g, sodium chloride 30 g, magnesium sulfate 0.5 g, calcium carbonate 0.1 g, glass powder 1.0 g and agar powder 15 g, and the pH is adjusted to 7.5-8.0.
The composition of the salt-resistant screening liquid culture medium is as follows: every 1000 mL distilled water contains glucose 5g, yeast extract 0.5 g, disodium hydrogen phosphate 2.0 g, ammonium sulfate 0.5 g, manganese sulfate 0.03 g, ferrous sulfate 0.03 g, sodium chloride 30 g, magnesium sulfate 0.5 g, calcium carbonate 0.1 g, glass powder 1.0 g and pH is adjusted to 7.5-8.0.
Example 2 morphological observations and physiological and Biochemical characterization of Strain K6
The morphology of strain K6 was observed using an oil microscope of Nikon inverted microscope.
The culture temperature of the strain K6 physiological and biochemical characteristic identification test is set to be 28 ℃. And meanwhile, the optimal temperature, the optimal growth pH value and the optimal salt concentration of the strain K6 are analyzed when the strain K6 grows on an LB solid medium.
The biological characteristics of strain K6 are: is round or oval, does not generate spores, and has a single cell size of (0.2 μm to 0.5 μm) × (1.2 μm to 1.5 μm) (FIG. 1). The colony is round, protruding, light yellow, opaque, moist, easy to pick and irregular in edge (figure 2) when solid cultured at 28 ℃.
The physiological and biochemical characteristics of strain K6 are: gram staining is negative, aerobic, the optimal growth temperature is 25-33 ℃, the optimal growth pH value is 6.5-8.0, and the optimal salt concentration is 1-5% (figure 3).
The result of determining the 16S rRNA gene sequence of the strain K6 obtained by screening shows that the gene length is 1392bp, and the corresponding nucleotide sequence is shown as SEQ ID NO. 1.
The strain selected was determined to be a strain of Brucella lupulus (Brucella lupini), brucella lupeus K6, by using the BLASTN program alignment and phylogenetic analysis of the United states bioengineering information center (National Center for Biotechnology Information, NCBI).
The 16S rDNA sequence of 15 sequences with higher homology is selected as a reference object, and a phylogenetic tree between the strain K6 and a reference strain is constructed by using Mega 7 software by adopting a proximity method (neighbor-Joining). In the evolutionary tree, strain K6 forms a separate intra-cluster evolutionary branch with model strain Brucella lupini strain NBRC 102587 of Brucella lupekoe (FIG. 7).
The composition of the liquid culture medium for observing the bacterial forms is as follows: every 1000 mL distilled water contains peptone 10 g, yeast powder 5 g and sodium chloride 30 g, and the pH is regulated to 7.5-8.0.
The composition of the solid culture medium for observing the bacterial forms is as follows: every 1000mL distilled water contains peptone 10g, yeast powder 5 g, sodium chloride 30 g and agar powder 15 g, and the pH is regulated to 7.5-8.0.
EXAMPLE 3 analysis of the Potassium and phosphorus-solubilizing and other Protoffees of Strain K6
The isolated single colony of the strain K6 is transferred into a test tube filled with 5mL liquid culture medium, the culture is oscillated for 24 h under the conditions of 28 ℃ and 180 rpm, 10 uL culture solution is inoculated on potassium-dissolving culture medium, the culture is carried out in a constant temperature incubator at 28 ℃ for 3 days, and whether transparent oil-drop-shaped colonies appear on a culture medium plate is observed. The results showed that strain K6 grew transparent oil drop-shaped colonies on the plates after 24 hours of cultivation in silicate bacteria solid medium, indicating that strain K6 was able to dissolve potassium feldspar and had potassium-dissolving properties (FIG. 4).
Transferring the isolated single colony of the strain K6 into a test tube filled with 5mL liquid culture medium, carrying out shaking culture at 28 ℃ and 180: 180 rpm for 24: 24h, taking 10: 10 uL seed liquid, inoculating the seed liquid onto an organic phosphorus solid culture medium, culturing in a constant temperature incubator at 28 ℃, and observing whether a degradation transparent ring exists around the colony. The results show that after the strain K6 is cultured in the organophosphorus solid medium for 2 days, obvious degradation transparent rings appear around the colony, and the degradation rings are obviously increased after the culture is continued, so that the strain K6 can quickly dissolve organophosphorus and has stronger phosphate dissolving characteristics (figure 5).
The isolated single colony of the strain K6 is transferred into a test tube filled with 5 mL liquid culture medium, and is subjected to shaking culture at 28 ℃ and 180: 180 rpm for 24: 24h, 10: 10 uL seed liquid is inoculated onto inorganic phosphorus solid culture medium, and the culture is carried out in a constant temperature incubator at 28 ℃. After 3 days of culture, a clear transparent degradation ring is generated around the colony, which indicates that the strain K6 can dissolve inorganic phosphorus and has phosphorus dissolving property (figure 6).
The composition of the potassium-dissolving culture medium is as follows: every 1000 mL distilled water contains sucrose 5g, ammonium sulfate 0.5g, yeast extract 0.5g, magnesium sulfate 0.3 g, disodium hydrogen phosphate 2g, ferrous sulfate 0.03 g, manganese sulfate 0.03 g, potassium feldspar 2g, agar powder 15 g and pH 7.5.
The composition of the organic phosphorus solid culture medium is as follows: each 1000 mL distilled water contains glucose 10 g, ammonium sulfate 0.5 g, sodium chloride 10 g, magnesium sulfate 0.3 g, manganese sulfate 0.03 g, potassium chloride 0.3 g, ferrous sulfate 0.03 g, lecithin 2.0 g, agar powder 15 g and pH 7.5.
The inorganic phosphorus solid culture medium comprises the following components in percentage by weight: each 1000 mL distilled water contains glucose 10g, ammonium sulfate 0.5g, sodium chloride 10g, magnesium sulfate 0.3 g, manganese sulfate 0.03 g, potassium chloride 0.3 g, ferrous sulfate 0.03 g, calcium phosphate 5.0 g, agar powder 15 g and pH 7.5.
EXAMPLE 4 16S rRNA Gene identification method of Strain K6
The total genomic DNA of isolated and purified strain K6 was extracted according to the instructions of BioTeKe bacterial genome extraction kit. The total genomic DNA extracted was detected by 1% agarose gel electrophoresis at 175V for 20 minutes. The 16S rRNA gene of the isolated strain was amplified using the upstream primer 27F shown in SEQ ID No.2 (5 '-3': AGAGTTTGATCCTGGCTCAG) and the downstream primer 1492R shown in SEQ ID No.3 (5 '-3': GGTTACCTTGTTACGACTT), and the PCR reaction system and conditions are shown in tables 1 and 2.
TABLE 1 16S rRNA Gene PCR reaction System of purified Strain K6
Table 2 PCR reaction program settings
The PCR products were detected by 1% agarose gel electrophoresis at 175V for 20 minutes. The PCR of the 16S rRNA gene was observed near the 1.5 kbp Marker band position using a gel imager, and after the desired band was excised, the PCR product was purified and recovered according to the instructions of the agarose gel recovery kit. And (5) sending the purified and recovered PCR product to a sequencing company for sequencing.
The result of measuring the 16S rRNA gene sequence of the strain K6 shows that the gene length is 1392 bp, and the corresponding nucleotide sequence is shown as SEQ ID NO. 1.
The 16S rRNA gene sequence obtained by sequencing was submitted to NCBI database (http:// www.ncbi.nlm.nih.gov) for BLASTN alignment, and the result shows that the similarity of the 16S rRNA of the strain to Brucella lupeus (Brucella lupini) NBRC 102587 is 99.87%. The 16S rDNA sequence of 15 sequences with higher homology is selected as a reference object, and a phylogenetic tree between the strain K6 and a reference strain is constructed by using Mega 7 software by adopting a proximity method (neighbor-Joining). In the evolutionary tree, strain K6 and Brucella lupekoe model strain Brucella lupinistrain NBRC 102587 are therefore designated Brucella lupekoe K6.
Example 5 application of Strain K6 to increasing available phosphorus and soluble Potassium in saline alkaline soil
(1) And (3) strain selection: brucella lupeus strain K6.
(2) Activating strains: inoculating the strain on a strain activated solid culture medium, and standing and culturing for 24-48 hours at the temperature of 28-30 ℃ for later use.
(3) Seed culture: and (3) picking the bacterial colony in the step (2), inoculating the bacterial colony into a liquid seed culture medium containing 5 mL, and culturing for 24-30 h at the temperature of 28-30 ℃ under the condition of 180-rpm.
(4) Fermentation culture: inoculating the seed liquid into a triangular flask (500 mL) containing 100 mL fermentation medium with an inoculum size of 1%, and culturing at 28-30 ℃ under 180 rpm conditions for 48 h to obtain a bacterial suspension containing brucella lupeus for later use.
(5) Increase the detection of soluble potassium and phosphorus in the saline-alkali soil: filling saline-alkali soil with the salt concentration of 3 per mill and pouring a 7 per mill NaCl solution into a flowerpot (30 cm multiplied by 24 multiplied by cm multiplied by 9 cm), and carrying out different gradient dilution on the fermentation-prepared brucella lupeum suspension to obtain brucella lupeum K6 suspension with two concentrations of 1-5 multiplied by 10 6 cfu/mL and 1-5 multiplied by 10 10 cfu/mL respectively; respectively applying two bacterial solutions with different concentrations of 20 mL into soil, and simultaneously taking inactivated bacterial solution as a control; and detecting the contents of available quick-acting potassium and available phosphorus in the soil after ten days. Each treatment was repeated three times.
The results show that the quick-acting potassium and the effective phosphorus content in the saline-alkali soil added with the K6 bacterial liquid are obviously increased compared with the control group through the detection of the physical and chemical indexes of the soil (Table 3), the quick-acting potassium content in the soil is increased by 43.7%, and the effective phosphorus content in the soil is increased by 32.9%.
TABLE 3 detection results of available phosphorus and soluble Potassium in Experimental soil
The strain activation solid culture medium comprises the following components: every 1000 mL distilled water contains peptone 10 g, yeast powder 5g, sodium chloride 30g and agar powder 15 g, and the pH is regulated to 7.5-8.0.
The liquid seed culture medium comprises the following components: every 1000mL distilled water contains peptone 10 g, yeast powder 5 g and sodium chloride 30 g, and the pH is regulated to 7.5-8.0.
The fermentation medium composition of the lupin brucella K6 is as follows: every 1000 mL tap water contains 10 g g of glucose, 2g of yeast powder, 10 g g of sodium chloride, 0.5 g g of potassium chloride, 3 g g of calcium phosphate, 0.05g of ferrous sulfate, 0.2g of magnesium sulfate and pH is regulated to 7.5-8.0.
Claims (8)
1. The lupeum (Brucella lupini) K6 with the salt tolerance and growth promotion functions is characterized in that the collection number of the lupeum K6 is CGMCC No. 27796, and the lupeum K6 is collected in the China general microbiological culture Collection center of China general microbiological culture Collection center (China) for 7 th month 4 of 2023.
2. Use of the lupeus lupulus K6 with salt tolerance and growth promotion function according to claim 1 for increasing quick-acting potassium and effective phosphorus in saline-alkali soil under salt stress conditions.
3. The use of lupeus lupulus K6 with salt tolerance and growth promotion function in increasing quick-acting potassium and available phosphorus in saline-alkali soil under salt stress condition, wherein the salt stress refers to saline-alkali soil with a salt concentration mass ratio of not less than 3 per mill in the soil.
4. Use of brucella lupeus K6 with salt tolerance and growth promotion function according to claim 2 or 3 for increasing the fast-acting potassium and available phosphorus in saline-alkali soil under salt stress conditions, comprising the steps of:
(1) Inoculating the activated brucella lupeus K6 seed solution into a fermentation liquid culture medium for culturing to obtain a brucella lupeus-containing suspension;
(2) Diluting the fermented brucella lupeus suspension, and applying the diluted brucella lupeus suspension into saline-alkali soil.
5. The application of the lupeum album strain K6 with the salt tolerance and growth promotion function to increase quick-acting potassium and effective phosphorus in saline-alkali soil under the condition of salt stress as claimed in claim 4, wherein in the step (1), the inoculation is carried out by using activated lupeum strain seed liquid according to an inoculation amount of 1-2% by volume.
6. The application of the lupin brucella K6 with the salt tolerance and growth promotion function in increasing quick-acting potassium and effective phosphorus in saline-alkali soil under the condition of salt stress as claimed in claim 4, wherein in the step (1), the fermentation liquid culture medium contains 10 g glucose, 2g yeast powder, 10 g sodium chloride, 0.5 g potassium chloride, 3g calcium phosphate, 0.05g ferrous sulfate, 0.2g magnesium sulfate and the pH value is adjusted to 7.5-8.0 in every 1000 mL tap water.
7. The use of the lupeus lupulus K6 with salt tolerance and growth promotion function for increasing quick-acting potassium and available phosphorus in saline-alkali soil under salt stress conditions, wherein in the step (1), the culture condition is that the culture is carried out for 24-48 hours under the conditions of 28-30 ℃ and 180 rpm.
8. The use of lupeum K6 with salt tolerance and growth promotion according to claim 4 for increasing the amount of fast-acting potassium and available phosphorus in saline-alkali soil under salt stress conditions, wherein in step (2), the concentration of the diluted lupeum suspension is 1X 10 6cfu/mL~1×1010 cfu/mL.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1754958A (en) * | 2004-09-30 | 2006-04-05 | 中国科学院动物研究所 | Degradation bacteria of one highly effective degrading organophosphorus pesticide and uses thereof |
| CN101818122A (en) * | 2009-07-29 | 2010-09-01 | 兰州理工大学 | Enterobacter bacteria for efficiently degrading organophosphorus pesticides and culturing method thereof |
| US9743673B1 (en) * | 2014-05-14 | 2017-08-29 | Iowa State University Research Foundation, Inc. | Modification of nitrogen-fixing rhizobacteria to enhance nodulated plant resistance |
| CN112867784A (en) * | 2018-09-21 | 2021-05-28 | 皮沃特生物股份有限公司 | Methods and compositions for improved phosphate solubilization |
| KR20220088068A (en) * | 2020-12-18 | 2022-06-27 | 대한민국(농촌진흥청장) | Brucella anthropi T16R-87 strain with plant growth promoting effect and osmotic stress resistance induction effect and use thereof |
| CN115011510A (en) * | 2022-05-20 | 2022-09-06 | 青岛农业大学 | Salt-tolerant growth-promoting stenotrophomonas maltophilia, microbial agent thereof and application thereof |
| CN115151632A (en) * | 2019-11-22 | 2022-10-04 | 诺维信生物农业公司 | Paenibacillus isolate and use thereof |
| CN116496921A (en) * | 2022-11-25 | 2023-07-28 | 华南农业大学 | Endophyte for dissolving phosphorus and potassium and growth promotion application thereof |
-
2023
- 2023-11-06 CN CN202311462230.5A patent/CN117467575B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1754958A (en) * | 2004-09-30 | 2006-04-05 | 中国科学院动物研究所 | Degradation bacteria of one highly effective degrading organophosphorus pesticide and uses thereof |
| CN101818122A (en) * | 2009-07-29 | 2010-09-01 | 兰州理工大学 | Enterobacter bacteria for efficiently degrading organophosphorus pesticides and culturing method thereof |
| US9743673B1 (en) * | 2014-05-14 | 2017-08-29 | Iowa State University Research Foundation, Inc. | Modification of nitrogen-fixing rhizobacteria to enhance nodulated plant resistance |
| CN112867784A (en) * | 2018-09-21 | 2021-05-28 | 皮沃特生物股份有限公司 | Methods and compositions for improved phosphate solubilization |
| CN115151632A (en) * | 2019-11-22 | 2022-10-04 | 诺维信生物农业公司 | Paenibacillus isolate and use thereof |
| KR20220088068A (en) * | 2020-12-18 | 2022-06-27 | 대한민국(농촌진흥청장) | Brucella anthropi T16R-87 strain with plant growth promoting effect and osmotic stress resistance induction effect and use thereof |
| CN115011510A (en) * | 2022-05-20 | 2022-09-06 | 青岛农业大学 | Salt-tolerant growth-promoting stenotrophomonas maltophilia, microbial agent thereof and application thereof |
| CN116496921A (en) * | 2022-11-25 | 2023-07-28 | 华南农业大学 | Endophyte for dissolving phosphorus and potassium and growth promotion application thereof |
Non-Patent Citations (3)
| Title |
|---|
| "Genetic diversity and phylogenetic relationships of bacteria belonging to the Ochrobactrum–Brucellagroup byrecAand 16S rRNA gene-based comparative sequence analysis";Holger C. Scholz et al.;《Systematic and Applied Microbiology》;20081231;第31卷;1-16 * |
| Nakagawa,Y.."Breuclla lupini strain NBRC 102587 16S ribosomal RNA, partial sequence, NCBI Reference Sequence: NR_114150.1".《GenBank》.2022,1-2. * |
| 苍白杆菌 B2 对甲基对硫磷降解途径研究;柏文琴 等;《农药学学报》;20041231;第6卷(第4期);48-54 * |
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