CN117385106A - Rapid calculation method of feeding amount, feeding method and feeding system - Google Patents
Rapid calculation method of feeding amount, feeding method and feeding system Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 40
- 238000004364 calculation method Methods 0.000 title claims description 17
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 143
- 238000000855 fermentation Methods 0.000 claims abstract description 136
- 230000004151 fermentation Effects 0.000 claims abstract description 136
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims abstract description 86
- VTHJTEIRLNZDEV-UHFFFAOYSA-L magnesium dihydroxide Chemical compound [OH-].[OH-].[Mg+2] VTHJTEIRLNZDEV-UHFFFAOYSA-L 0.000 claims abstract description 77
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims abstract description 71
- 239000000347 magnesium hydroxide Substances 0.000 claims abstract description 70
- 229910001862 magnesium hydroxide Inorganic materials 0.000 claims abstract description 70
- 229910021529 ammonia Inorganic materials 0.000 claims abstract description 43
- 238000006243 chemical reaction Methods 0.000 claims abstract description 34
- 238000004519 manufacturing process Methods 0.000 claims abstract description 32
- 239000001384 succinic acid Substances 0.000 claims abstract description 32
- 230000003204 osmotic effect Effects 0.000 claims abstract description 15
- 238000007599 discharging Methods 0.000 claims abstract description 14
- 238000012544 monitoring process Methods 0.000 claims abstract description 9
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 28
- 239000004202 carbamide Substances 0.000 claims description 28
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims description 21
- 229910001425 magnesium ion Inorganic materials 0.000 claims description 21
- 239000000725 suspension Substances 0.000 claims description 19
- 239000000376 reactant Substances 0.000 claims description 17
- 230000003472 neutralizing effect Effects 0.000 claims description 15
- 150000002500 ions Chemical class 0.000 claims description 14
- 239000007795 chemical reaction product Substances 0.000 claims description 11
- 238000002474 experimental method Methods 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 9
- 238000003860 storage Methods 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 9
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- 229910001415 sodium ion Inorganic materials 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 abstract description 8
- 238000006386 neutralization reaction Methods 0.000 abstract description 6
- -1 ammonium ions Chemical class 0.000 abstract description 5
- 238000004904 shortening Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 25
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- 108010046334 Urease Proteins 0.000 description 4
- 230000001737 promoting effect Effects 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 239000003054 catalyst Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 239000012670 alkaline solution Substances 0.000 description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 239000003014 ion exchange membrane Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- YLQBMQCUIZJEEH-UHFFFAOYSA-N Furan Chemical group C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 1
- 229920000180 alkyd Polymers 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 125000000623 heterocyclic group Chemical group 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q3/00—Condition responsive control processes
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
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Abstract
The invention relates to a rapid charging amount calculating method, a charging method and a charging system, and relates to the technical field of preparing succinic acid by fermentation, wherein the rapid charging amount calculating method comprises the steps of fermentation reaction prediction, determination of neutralizer, determination of strain tolerance, determination of ammonia consumption, determination of magnesium hydroxide consumption and the like; the charging method comprises the steps of monitoring, threshold judgment, charging, tank discharging judgment, tank discharging and the like; the feeding system comprises a fermentation tank, a pH value monitor, a central control system, a first feeding system, a second feeding system and a third feeding system. The invention can rapidly calculate the minimum dosage of ammonia and magnesium hydroxide, thus shortening the preparation time before production, reducing the influence of ammonium ions and osmotic pressure on the growth of the strain during production, improving the fermentation efficiency of the strain, and simultaneously using sodium hydroxide as a neutralization substitute of magnesium hydroxide, thereby further reducing the production cost.
Description
Technical Field
The invention relates to the technical field of succinic acid preparation by fermentation, in particular to a rapid calculation method of a feeding amount, a feeding method and a feeding system.
Background
Succinic acid is an important intermediate for preparing five-membered heterocyclic rings, and can be widely used for processing and producing furan ring compounds, medicaments, dyes and alkyd resins due to the special molecular structure of the succinic acid. Succinic acid has various production methods, wherein the microbial fermentation method has the advantages of low energy consumption, high yield, small pollution, capability of producing food-grade products and the like, so that the method is gradually replacing other methods for producing succinic acid.
The optimal fermentation pH value of the strain for fermenting the succinic acid is about 6.5, and the succinic acid generated by fermentation can reduce the pH value in the fermentation tank because the succinic acid is acidic, so that the subsequent fermentation efficiency of the strain is reduced, and the core process of the process for preparing the succinic acid by the fermentation method is how to control the pH value in the fermentation tank.
At present, alkaline materials are mainly put into a fermentation tank so as to control the pH value in the fermentation tank, and the alkaline materials mainly comprise ammonia water, sodium hydroxide, magnesium hydroxide and the like, but various alkaline materials have limitations.
Ammonia water can generate ammonium ions after reacting with succinic acid, and low concentration of ammonium ions can promote the growth of bacterial strains, but high concentration of ammonium ions can form an acidic environment in bacterial strains, so that bacterial strains die. Sodium ions are generated after the sodium hydroxide reacts with the succinic acid, and the sodium ions can increase the osmotic pressure in the fermentation tank, so that the strain is dead. Magnesium ions are generated after the magnesium hydroxide reacts with the succinic acid, the magnesium ions can promote the generation of enzymes, so that the fermentation is accelerated, and the magnesium ions do not form larger osmotic pressure like sodium ions, so that bacterial strain death is not easy to cause; however, the solubility of magnesium hydroxide is low, and if the magnesium hydroxide solution is added to neutralize succinic acid, the reaction solution in the fermentation tank can be diluted, so that the concentration of generated succinic acid is low; if magnesium hydroxide solid powder is added to neutralize succinic acid, the magnesium hydroxide powder is difficult to sterilize, so that the fermentation cost is increased sharply, the stability of the strain in the fermentation tank is difficult to ensure, and the fermentation failure is easy to cause; and after the succinic acid is fermented, the succinate is in the fermentation broth, the succinic acid can be prepared by exchanging through an ion exchange membrane, magnesium ions are divalent ions, and the exchange efficiency is low when the ion exchange membrane is used for exchanging, so that the cost for preparing the succinic acid is increased. Therefore, at present, when the succinic acid generated by the neutralization reaction is neutralized, two or more neutralizing agents are often selected for neutralization, so that the production cost of the succinic acid is reduced.
When two or more neutralizing agents are adopted to neutralize the succinic acid at present, a large amount of experiments are needed to obtain the use data of the neutralizing agents, the fermentation strains are diversified, and different fermentation strains can be put into production only by carrying out a large amount of experiments again, so that the time required by early-stage preparation is greatly increased, and the early-stage experiment cost is increased.
Disclosure of Invention
In order to reduce the preparation time of the primary fermentation and shorten the experiment cost, the invention provides a rapid calculation method of the feeding amount, a feeding method and a feeding system.
In a first aspect, the invention provides a method for rapidly calculating a feeding amount, which adopts the following technical scheme:
a rapid calculation method of feeding amount comprises the following steps:
and (3) fermentation reaction prediction: obtaining the feeding amount as the fermentation reactant, determining the reaction product according to the reaction characteristic of the strain and the feeding amount of the reactant, and calculating the output of the reaction productAfter which the production of the reaction product is based on +.>Calculate H + Yield mass->；
And (3) determining a neutralizer: ammonia and magnesium hydroxide are used as neutralizing agents for neutralizing succinic acid;
determination of strain tolerance: obtaining NH according to experiments 4 + Concentration of substance at the beginning of affecting Strain Activity；
Determination of ammonia consumption: predicting the volume V of the solution after the completion of the reaction, and adding the total amount of ammonia to the fermenterLess than or equal to->；
Determining the dosage of magnesium hydroxide: setting the ion concentration of magnesium ion after the reaction is completed asThe method comprises the steps of carrying out a first treatment on the surface of the The ion concentration of magnesium ion after the completion of the reaction is +.>The calculation model of (2) is as follows:
；
total amount of magnesium hydroxide fed into the fermenterIs->。
By adopting the technical scheme, the strain batch is replaced or the strain is replacedWhen the strain variety is used, only the NH of the current strain pair is obtained in a laboratory 4 + The sensitivity of the catalyst is enough, and the dosage of ammonia and magnesium hydroxide can be calculated quickly, so that the preparation time before production can be shortened, and NH can be reduced during production 4 + The effect on the growth of the strain is improved, the fermentation efficiency of the strain is improved, and ammonia is used as a neutralization substitute of magnesium hydroxide, so that the production cost can be reduced.
Optionally, in the step of determining the neutralizing agent, sodium hydroxide is also used as a neutralizing agent for neutralizing succinic acid;
in the step of determining the tolerance of the strain, the total substance concentration at which the osmotic pressure starts to influence the activity of the strain is also obtained；
In the magnesium hydroxide dosage determining step, the concentration of sodium ions after the reaction is finished is also set asThe method comprises the steps of carrying out a first treatment on the surface of the The concentration of sodium ions after completion of the reaction is +.>For calculating the total amount of sodium hydroxide fed to the fermenter +.>The method comprises the steps of carrying out a first treatment on the surface of the And the amount of feed which is not used as a fermentation reactant is obtained>And calculates the ion concentration which is not used as a fermentation reactant after the completion of the reaction +.>；
The ion concentration of magnesium ions after the reaction is completed isThe updated calculation model is as follows:
；（1）
；（2）
taking the two sides of (1) equalIs the lowest concentration of magnesium ions;
total amount of magnesium hydroxide fed into the fermenterGreater than or equal to->；
The method also comprises a sodium hydroxide dosage determining step;
determining the dosage of sodium hydroxide: total amount of sodium hydroxide fed into the fermenterIs->。
By adopting the technical scheme, when the strain batch is replaced or the strain variety is replaced, the sensitivity degree of the current strain to the osmotic pressure is also obtained in a laboratory, and then the minimum dosage of magnesium hydroxide can be rapidly calculated, so that the preparation time before production can be shortened, the influence of the osmotic pressure on the growth of the strain can be reduced during production, the fermentation efficiency of the strain is improved, and meanwhile, the sodium hydroxide is used as a neutralization substitute of the magnesium hydroxide, so that the production cost can be further reduced.
In a second aspect, the invention provides a feeding method, which adopts the following technical scheme:
a method of charging comprising the steps of:
monitoring: monitoring the pH value in the fermentation tank in real time;
threshold judgment: setting a first pH threshold and a second pH threshold, wherein the first pH threshold is smaller than the second pH threshold; if the pH value in the fermentation tank is smaller than the first pH threshold value, executing a feeding step; stopping the feeding step if the pH value in the fermentation tank is greater than the second pH threshold value;
feeding: total amount of ammonia calculated according to the first aspectTotal amount of magnesium hydroxide->Total amount of sodium hydroxideDetermining the adding proportion, and the final adding proportion of ammonia, magnesium hydroxide and sodium hydroxide is->；
And (5) tank discharge judgment: setting a third pH threshold, and executing a tank discharging step if the ammonia, the magnesium hydroxide and the sodium hydroxide are completely fed and the pH value in the fermentation tank is smaller than the third pH threshold;
and (3) tank discharge: and taking out the solution in the fermentation tank to finish the fermentation step.
By adopting the technical scheme, when the succinic acid is produced by using a fermentation method, the final adding proportion of ammonia, magnesium hydroxide and sodium hydroxide is as followsFeeding can reduce NH 4 + And the influence of osmotic pressure on the growth of the strain, so that the fermentation efficiency of the strain is improved, and the dosage of magnesium hydroxide can be reduced to the maximum extent, so that the cost required for sterilizing and purifying succinic acid is reduced.
Optionally, in the feeding step, ammonia and magnesium hydroxide are fed first, and then sodium hydroxide is fed.
By adopting the technical scheme, due to small amount of NH 4 + The growth of the strain can be promoted, and magnesium ions can promote the generation of enzymes, so that the fermentation rate of the strain is improved; therefore, ammonia is added in advance of the reactionAnd magnesium hydroxide to promote fermentation reaction, raise fermentation efficiency, shorten fermentation time and reduce production cost.
Optionally, in the feeding step, urea is used as an ammonia source for feeding, and the feeding amount of urea is as follows。
By adopting the technical scheme, most of fermentation strains can secrete urease, urea is decomposed by the urease to produce ammonia, succinic acid produced by fermentation is neutralized, carbon dioxide can be produced when the urea is decomposed, and a good anoxic environment is provided for the fermentation strains so as to promote fermentation.
Optionally, in the feeding step, ammonia and sodium hydroxide are fed in a solution state, and magnesium hydroxide is fed in a suspension state.
Through adopting above-mentioned technical scheme, ammonia and sodium hydroxide all have higher solubility to put in the form of solution and to put in the dilution degree that can reduce succinic acid that fermentation produced, and the solubility of magnesium hydrate is at the bottom, uses the mode of suspension to put in, makes things convenient for the sterilization of magnesium hydrate, and then has reduced the probability that fermentation environment was destroyed in the fermentation cylinder, and the magnesium hydrate suspension is difficult to appear eliminating the killing angle simultaneously, has reduced the energy consumption when disinfecting to the magnesium hydrate. The magnesium hydroxide suspension is used for neutralizing the succinic acid, so that the utilization rate of the magnesium hydroxide can be improved, and the production cost is further reduced.
In a third aspect, the present invention provides a feeding system, which adopts the following technical scheme:
a feeding system comprises a fermentation tank, a pH value monitor, a central control system, a first feeding system, a second feeding system and a third feeding system;
a fermentation tank: for fermentation;
pH monitor: the device is arranged in the fermentation tank and is used for monitoring the pH value in the fermentation tank;
and the central control system comprises: the input end is connected with the pH value monitor and used for controlling feeding;
first feed system: the feeding end is connected with the urea solution storage tank, the discharging end is connected with the fermentation tank, and the input end is connected with the central control system and is used for adding urea solution into the fermentation tank;
second feeding system: the feeding end is connected with the magnesium hydroxide suspension storage tank, the discharging end is connected with the fermentation tank, and the input end is connected with the central control system and is used for filling magnesium hydroxide suspension into the fermentation tank;
third flow-adding system: the feeding end is connected with a sodium hydroxide solution storage tank, the discharging end is connected with the fermentation tank, and the input end is connected with a central control system for adding sodium hydroxide solution into the fermentation tank.
By adopting the technical scheme, the pH value monitor monitors the pH value in the fermentation tank in real time, and conveys the pH value to the central control system for judgment, when the pH value in the fermentation tank is lower than a first pH threshold value, the central control system controls the first feeding system, the second feeding system and the third feeding system to supplement alkaline solution into the fermentation tank, so that the pH value in the fermentation tank is maintained between the first pH threshold value and the second pH threshold value, and further the strain has higher fermentation efficiency.
Optionally, the output ends of the first flow adding system, the second flow adding system and the third flow adding system are connected with a central control system for outputting the filling amount to the central control system, and the central control system also controls the filling amount of the sodium hydroxide solution according to the filling amounts of the urea solution and the magnesium hydroxide suspension.
By adopting the technical scheme, the urea solution and the magnesium hydroxide suspension are fed by the first feeding system and the second feeding system under the control of the central control system, and a small amount of NH is used 4 + The growth of the strain can be promoted, and magnesium ions can promote the generation of enzymes, so that the fermentation rate of the strain is improved; therefore, ammonia and magnesium hydroxide are firstly added in the early stage of the reaction to promote the fermentation reaction, improve the fermentation efficiency, shorten the fermentation time and further reduce the production cost; meanwhile, the utilization rate of magnesium hydroxide can be improved, and the production cost is reduced.
In summary, the present invention includes at least one of the following beneficial technical effects:
1. through the setting of the rapid calculation method of the feeding amount, the minimum amount of ammonia and magnesium hydroxide can be rapidly calculated, so that the preparation time before production can be shortened, the influence of ammonium ions and osmotic pressure on the growth of the strain can be reduced during production, the fermentation efficiency of the strain is improved, and meanwhile, sodium hydroxide is used as a neutralization substitute of magnesium hydroxide, so that the production cost can be further reduced.
2. By adding ammonia and magnesium hydroxide first and then adding sodium hydroxide, a small amount of NH can be passed 4 + Promoting the growth of the strain, and simultaneously using magnesium ions to promote the generation of enzymes so as to further improve the fermentation rate of the strain; shortens the fermentation time and further reduces the production cost.
3. By using urea as the ammonia source, urease produces ammonia as well as carbon dioxide upon decomposing urea, which can provide a good anoxic environment for the fermentation strain to promote fermentation.
Detailed Description
Example 1: the embodiment discloses a rapid calculation method of feeding amount, which comprises the following steps:
s1-1: and (3) fermentation reaction prediction: obtaining the feeding amount as the fermentation reactant, determining the reaction product according to the reaction characteristic of the strain and the feeding amount of the reactant, and calculating the output of the reaction productAfter which the production of the reaction product is based on +.>Calculate H + Yield mass->。
The current strains can basically consume reactants completely when the succinic acid is generated by fermentation, so that the calculation is performed according to the standard that the reactants are completely consumed when the output of reaction products is predicted. If the replaced strain belongs to a strain which cannot completely consume the reactant, the proportion of the strain capable of consuming the reactant is obtained, and then the yield of the reaction product is calculated by taking the feeding amount of the reactant and the proportion of the consumed reactant as the reference.
S1-2: and (3) determining a neutralizer: ammonia, sodium hydroxide and magnesium hydroxide were used as neutralizing agents for neutralizing succinic acid.
S1-3: determination of strain tolerance: obtaining NH according to experiments 4 + Concentration of substance at the beginning of affecting Strain ActivityThe method comprises the steps of carrying out a first treatment on the surface of the Obtaining the total substance concentration at which osmotic pressure starts to affect the activity of the strain +.>。
S1-4: determination of ammonia consumption: predicting the volume V of the solution after the completion of the reaction, and adding the total amount of ammonia to the fermenterLess than or equal to->. Obtaining NH according to experiments 4 + Substance concentration at the beginning of affecting the Activity of the Strain +.>NH during fermentation reaction 4 + Is lower than the substance concentration +.>When NH 4 + Can provide nitrogen source for the strain, and further promote the growth of the strain so as to improve the fermentation efficiency.
S1-5: determining the dosage of magnesium hydroxide: setting the ion concentration of magnesium ion after the reaction is completed asThe method comprises the steps of carrying out a first treatment on the surface of the The concentration of sodium ion after completion of the reaction was set to +.>The method comprises the steps of carrying out a first treatment on the surface of the Obtain not asAddition of fermentation reactants->And calculates the ion concentration which is not used as a fermentation reactant after the completion of the reaction +.>。
The ion concentration of magnesium ions after the reaction is completed isThe calculation model of (2) is as follows:
；（1）
；（2）
taking the two sides of (1) equalIs the lowest concentration of magnesium ions;
total amount of magnesium hydroxide fed into the fermenterGreater than or equal to->。
When the two sides are equal in formula (1), it is proved that the concentration of ions in the fermenter is the total substance concentration at which osmotic pressure begins to affect the activity of the strainThe total amount of magnesium hydroxide added at this time +.>Is the lowest feeding amount of magnesium hydroxide, and when the feeding amount of magnesium hydroxide is more, the osmotic pressure in the fermentation tank is reduced because magnesium ions are divalent ions.
S1-6：Determining the dosage of sodium hydroxide; calculating the total amount of sodium hydroxide fed into the fermenterTotal amount of sodium hydroxide added->Is->。
The implementation principle of the rapid calculation method of the feeding amount of the embodiment is as follows:
when changing strain batches or strain varieties, experiments need to be carried out on the strain to determine the optimal reaction environment; since succinic acid produced by fermentation of the strain affects the progress of fermentation, it is necessary to neutralize succinic acid with an alkaline material. The alkaline solution has more selectable items, and if the optimal reaction ratio is obtained through sequential experiments, the production process can be seriously influenced, so that the feeding amount of alkaline materials is rapidly calculated according to the characteristics of bacterial strains, and the production process is further accelerated.
In the calculation, the NH of the current strain pair is acquired in a laboratory 4 + The sensitivity of the catalyst to osmotic pressure and the sensitivity of the catalyst to osmotic pressure, and then the dosage of ammonia, sodium hydroxide and magnesium hydroxide can be rapidly calculated; in this way, the preparation time before production can be shortened, and NH can be reduced during production 4 + The method has the advantages of improving the fermentation efficiency of the strain due to the influence on the growth of the strain, simultaneously calculating the minimum dosage of magnesium hydroxide rapidly, and reducing the production cost.
Example 2: the embodiment discloses a feeding method, which comprises the following steps:
s2-1: monitoring: the pH in the fermenter was monitored in real time.
S2-2: threshold judgment: setting a first pH threshold and a second pH threshold, wherein the first pH threshold is smaller than the second pH threshold; if the pH value in the fermentation tank is smaller than the first pH threshold value, executing a feeding step; and stopping the feeding step if the pH value in the fermentation tank is greater than the second pH threshold value.
S2-3: throwing inAnd (3) material: total amount of ammonia calculated according to example 1Total amount of magnesium hydroxide->Total amount of sodium hydroxide->Determining the adding proportion, and the final adding proportion of ammonia, magnesium hydroxide and sodium hydroxide is->. When the urea is fed into the fermentation tank, urea is taken as an ammonia source for feeding, and the feeding amount of the urea is +.>Urea and magnesium hydroxide are put first, and then sodium hydroxide is put in; the added urea and sodium hydroxide are used as solutions, and the added magnesium hydroxide is used as suspension.
S2-4: and (5) tank discharge judgment: setting a third pH threshold, and executing a tank discharging step if the ammonia, the magnesium hydroxide and the sodium hydroxide are completely fed and the pH value in the fermentation tank is smaller than the third pH threshold;
s2-5: and (3) tank discharge: and taking out the solution in the fermentation tank to finish the fermentation step.
The implementation principle of the feeding method of the embodiment is as follows:
when the succinic acid is produced by using the fermentation method, the final adding proportion of ammonia, magnesium hydroxide and sodium hydroxide is as followsFeeding can reduce NH 4 + And the influence of osmotic pressure on the growth of the strain, so that the fermentation efficiency of the strain is improved, and the dosage of magnesium hydroxide can be reduced to the maximum extent, so that the cost required for sterilizing and purifying succinic acid is reduced.
In addition, in the case of adding ammonia and magnesium hydroxide, NH can be used 4 + Promoting growth of strainsThe magnesium ions can promote the generation of enzymes, further improve the fermentation rate of the strain, shorten the fermentation time, further reduce the production cost, firstly, the magnesium hydroxide suspension is used for neutralizing the succinic acid, and further improve the utilization rate of the magnesium hydroxide, and further reduce the production cost; urea is taken as an ammonia source for feeding, urea is decomposed by urease to generate ammonia, succinic acid generated by fermentation is neutralized, carbon dioxide can be generated when the urea is decomposed, and a good anoxic environment is provided for a fermentation strain so as to promote the fermentation reaction.
Example 3: the embodiment discloses a feeding system, which comprises a fermentation tank, a pH value monitor, a central control system, a first feeding system, a second feeding system and a third feeding system;
a fermentation tank: for fermentation;
pH monitor: the device is arranged in the fermentation tank and is used for monitoring the pH value in the fermentation tank;
and the central control system comprises: the input end is connected with the pH value monitor and is used for controlling the filling amount of the sodium hydroxide solution according to the filling amounts of the urea solution and the magnesium hydroxide suspension;
first feed system: the feeding end is connected with the urea solution storage tank, the discharging end is connected with the fermentation tank, the input end and the output end are both connected with the central control system and are used for injecting urea solution into the fermentation tank and simultaneously outputting the filling amount of the first flow filling system to the central control system;
second feeding system: the feeding end is connected with the magnesium hydroxide suspension storage tank, the discharging end is connected with the fermentation tank, the input end and the output end are both connected with the central control system and are used for filling magnesium hydroxide suspension into the fermentation tank and simultaneously outputting the filling quantity of the second feeding system to the central control system;
third flow-adding system: the feeding end is connected with the sodium hydroxide solution storage tank, the discharging end is connected with the fermentation tank, the input end and the output end are both connected with the central control system and are used for adding sodium hydroxide solution into the fermentation tank and simultaneously outputting the filling quantity of the third flow adding system to the central control system.
The implementation principle of the charging system of the embodiment is as follows:
the pH value in the fermentation tank is monitored in real time by using a pH value monitor, the pH value is conveyed to a central control system for judgment, when the pH value in the fermentation tank is lower than a first pH threshold value, the central control system firstly controls a first feeding system and a second feeding system to feed urea solution and magnesium hydroxide suspension into the fermentation tank, so that the pH value in the fermentation tank is maintained between the first pH threshold value and the second pH threshold value, the strain has higher fermentation efficiency, and the strain can pass through NH (NH) 4 + Promoting the growth of the strain, and promoting the generation of enzyme through magnesium ions so as to further improve the fermentation rate of the strain; after the urea solution and the magnesium hydroxide suspension are added to a certain amount in proportion, the central control system controls the second feeding system to feed the sodium hydroxide solution, so that the production cost is reduced.
The above embodiments are not intended to limit the scope of the present invention, so: all equivalent changes in structure, shape and principle of the invention should be covered in the scope of protection of the invention.
Claims (8)
1. A rapid calculation method for feeding amount is characterized in that: the method comprises the following steps:
and (3) fermentation reaction prediction: obtaining the feeding amount as the fermentation reactant, determining the reaction product according to the reaction characteristic of the strain and the feeding amount of the reactant, and calculating the output of the reaction productAfter which the production of the reaction product is based on +.>Calculate H + Yield mass->；
And (3) determining a neutralizer: ammonia and magnesium hydroxide are used as neutralizing agents for neutralizing succinic acid;
determination of strain tolerance: obtaining NH according to experiments 4 + Substances which initially affect the activity of the strainConcentration of；
Determination of ammonia consumption: predicting the volume V of the solution after the completion of the reaction, and adding the total amount of ammonia to the fermenterLess than or equal to；
Determining the dosage of magnesium hydroxide: setting the ion concentration of magnesium ion after the reaction is completed asThe method comprises the steps of carrying out a first treatment on the surface of the The ion concentration of magnesium ion after the completion of the reaction is +.>The calculation model of (2) is as follows:
；
total amount of magnesium hydroxide fed into the fermenterIs->。
2. The method for rapidly calculating the feeding amount according to claim 1, wherein: in the step of determining the neutralizer, sodium hydroxide is also used as the neutralizer for neutralizing succinic acid;
in the step of determining the tolerance of the strain, the total substance concentration at which the osmotic pressure starts to influence the activity of the strain is also obtained；
In the magnesium hydroxide dosage determining step, the concentration of sodium ions after the reaction is finished is also set asThe method comprises the steps of carrying out a first treatment on the surface of the The concentration of sodium ions after completion of the reaction is +.>For calculating the total amount of sodium hydroxide fed to the fermenter +.>The method comprises the steps of carrying out a first treatment on the surface of the And the amount of feed which is not used as a fermentation reactant is obtained>And calculates the ion concentration which is not used as a fermentation reactant after the completion of the reaction +.>；
The ion concentration of magnesium ions after the reaction is completed isThe updated calculation model is as follows:
；（1）
；（2）
taking the two sides of (1) equalIs the lowest concentration of magnesium ions;
total amount of magnesium hydroxide fed into the fermenterGreater than or equal to/>；
The method also comprises a sodium hydroxide dosage determining step;
determining the dosage of sodium hydroxide: total amount of sodium hydroxide fed into the fermenterIs->。
3. A method of charging, characterized by: the method comprises the following steps:
monitoring: monitoring the pH value in the fermentation tank in real time;
threshold judgment: setting a first pH threshold and a second pH threshold, wherein the first pH threshold is smaller than the second pH threshold; if the pH value in the fermentation tank is smaller than the first pH threshold value, executing a feeding step; stopping the feeding step if the pH value in the fermentation tank is greater than the second pH threshold value;
feeding: the total amount of ammonia calculated by the rapid charge amount calculation method according to claim 2Total amount of magnesium hydroxide->Total amount of sodium hydroxide->Determining the adding proportion, wherein the final adding proportion of ammonia, magnesium hydroxide and sodium hydroxide is；
And (5) tank discharge judgment: setting a third pH threshold, and executing a tank discharging step if the ammonia, the magnesium hydroxide and the sodium hydroxide are completely fed and the pH value in the fermentation tank is smaller than the third pH threshold;
and (3) tank discharge: and taking out the solution in the fermentation tank to finish the fermentation step.
4. A charging method according to claim 3, wherein: in the feeding step, ammonia and magnesium hydroxide are firstly fed, and then sodium hydroxide is fed.
5. A charging method according to claim 4, wherein: in the feeding step, urea is used as an ammonia source for feeding, and the feeding amount of the urea is as follows。
6. A charging method according to claim 5, wherein: in the feeding step, ammonia and sodium hydroxide are fed in a solution state, and magnesium hydroxide is fed in a suspension state.
7. A charging system for applying the charging method according to any one of claims 3-6, characterized in that: comprises a fermentation tank, a pH value monitor, a central control system, a first feeding system, a second feeding system and a third feeding system;
a fermentation tank: for fermentation;
pH monitor: the device is arranged in the fermentation tank and is used for monitoring the pH value in the fermentation tank;
and the central control system comprises: the input end is connected with the pH value monitor and used for controlling feeding;
first feed system: the feeding end is connected with the urea solution storage tank, the discharging end is connected with the fermentation tank, and the input end is connected with the central control system and is used for adding urea solution into the fermentation tank;
second feeding system: the feeding end is connected with the magnesium hydroxide suspension storage tank, the discharging end is connected with the fermentation tank, and the input end is connected with the central control system and is used for filling magnesium hydroxide suspension into the fermentation tank;
third flow-adding system: the feeding end is connected with a sodium hydroxide solution storage tank, the discharging end is connected with the fermentation tank, and the input end is connected with a central control system for adding sodium hydroxide solution into the fermentation tank.
8. A loading system as defined in claim 7, wherein: the output ends of the first flow adding system, the second flow adding system and the third flow adding system are connected with a central control system and used for outputting the filling amount to the central control system, and the central control system also controls the filling amount of the sodium hydroxide solution according to the filling amounts of the urea solution and the magnesium hydroxide suspension.
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