CN117378317A - Method for improving germination rate of aged seeds of clove - Google Patents
Method for improving germination rate of aged seeds of clove Download PDFInfo
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- CN117378317A CN117378317A CN202311685571.9A CN202311685571A CN117378317A CN 117378317 A CN117378317 A CN 117378317A CN 202311685571 A CN202311685571 A CN 202311685571A CN 117378317 A CN117378317 A CN 117378317A
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- 230000035784 germination Effects 0.000 title claims abstract description 192
- 244000223014 Syzygium aromaticum Species 0.000 title claims abstract description 78
- 235000016639 Syzygium aromaticum Nutrition 0.000 title claims abstract description 78
- 238000000034 method Methods 0.000 title claims abstract description 25
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 claims abstract description 110
- 238000002791 soaking Methods 0.000 claims abstract description 57
- 229960001338 colchicine Drugs 0.000 claims abstract description 55
- 239000004576 sand Substances 0.000 claims abstract description 54
- 238000009331 sowing Methods 0.000 claims abstract description 53
- 238000011282 treatment Methods 0.000 claims abstract description 48
- 230000005855 radiation Effects 0.000 claims abstract description 38
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims abstract description 33
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 claims abstract description 33
- 229960003237 betaine Drugs 0.000 claims abstract description 33
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 claims abstract description 31
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims abstract description 31
- 229940095100 fulvic acid Drugs 0.000 claims abstract description 31
- 239000002509 fulvic acid Substances 0.000 claims abstract description 31
- 230000005251 gamma ray Effects 0.000 claims abstract description 20
- 238000001035 drying Methods 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 87
- 239000000243 solution Substances 0.000 claims description 51
- 230000001502 supplementing effect Effects 0.000 claims description 43
- 239000011259 mixed solution Substances 0.000 claims description 24
- 239000000203 mixture Substances 0.000 claims description 7
- 229940023579 anhydrous betaine Drugs 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 abstract description 4
- 238000005057 refrigeration Methods 0.000 abstract 1
- 241000628997 Flos Species 0.000 description 53
- 230000001276 controlling effect Effects 0.000 description 27
- 230000000694 effects Effects 0.000 description 9
- 230000001737 promoting effect Effects 0.000 description 5
- 230000032683 aging Effects 0.000 description 3
- 230000002411 adverse Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000011369 optimal treatment Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000207834 Oleaceae Species 0.000 description 1
- 241001104043 Syringa Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for improving germination rate of clove aged seeds, belonging to the technical field of plant planting, wherein Co is carried out on the clove aged seeds subjected to low-temperature refrigeration treatment 60 Gamma-ray radiation, sun-drying, soaking in colchicine, accelerating germination in sand mixed with betaine and fulvic acid, and sowing by selecting white seeds; the invention can improve the germination rate of the clove aged seeds by 26.04%.
Description
Technical Field
The invention relates to the technical field of plant planting, in particular to a method for improving germination rate of aged seeds of clove.
Background
Flos Caryophylli belongs to plants of Syringa of Oleaceae, is a traditional flower of China, and has high ornamental value and medicinal value. The breeding modes of the clove are various, and the sowing and breeding application is the most widely. The clove seeding is generally carried out in spring, most seeds collected in autumn in the previous year are selected, and the seeds are aged along with the extension of the storage time, namely the natural decline of the vitality of the seeds is a problem, especially when the storage condition is improper, the seeds are easy to age, so that the germination rate of the clove seeds is reduced, and the germination rate is increased along with the extension of the storage time.
Aiming at the aging of the clove seeds, the main method is to treat the aged clove seeds by chemical reagents so as to provide nutrient elements for the aged clove seeds and achieve the aim of improving the germination rate of the aged clove seeds, but the method has limited effect of improving the germination rate of the aged clove seeds; currently, there is no other more effective treatment for aged seeds of clove.
Disclosure of Invention
Aiming at the defects existing in the prior art, the invention provides a method for improving the germination rate of the aged seeds of the clove, which can effectively improve the germination rate of the aged seeds of the clove.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a method for improving germination rate of aging seeds of flos Caryophylli comprises using Co to aging seeds of flos Caryophylli 60 Radiating with gamma rays, sun-drying, soaking with colchicine, adding into sand containing betaine and fulvic acid mixed solution, accelerating germination, and timely supplementing waterWhen the seeds are exposed and white after 4-6d, selecting the exposed and white seeds for sowing;
the aged seeds of the clove are seeds of the clove stored for 3 years at low temperature.
Sun-drying the seeds within 1 week after the radiation treatment for 4 months and the last ten days, 8 am in sunny days is selected: 00 to 14: 00; airing the irradiated seeds in the sun, wherein the paving thickness is suitable for the seeds not to be blocked;
preferably, a method for improving germination rate of aged seeds of clove uses Co 60 The method comprises the steps of (1) radiating aged seeds of clove by gamma rays, controlling the radiation dose to be 20-60Gy, completely soaking the seeds in colchicine solution with the mass fraction of 0.3% for 5 hours after sun-drying, accelerating germination in sand containing a mixed solution of betaine and fulvic acid at the temperature of 23-27 ℃, and sowing after the seeds are exposed to white;
preferably, a method for improving germination rate of aged seeds of clove uses Co 60 The method comprises the steps of (1) radiating aged seeds of clove by gamma rays, controlling the radiation dose to be 20-100Gy, completely soaking the seeds in colchicine solution with the mass fraction of 0.05% for 10 hours after sun-drying, accelerating germination in sand containing a mixed solution of betaine and fulvic acid at the temperature of 23-27 ℃, and sowing after the seeds are exposed to white;
preferably, a method for improving germination rate of aged seeds of clove uses Co 60 The method comprises the steps of (1) radiating aged seeds of clove by gamma rays, controlling the radiation dose to be 20-100Gy, fully soaking the seeds in colchicine solution with the mass fraction of 0.05-0.1% for 5 hours after sun-drying, accelerating germination in sand containing a mixed solution of betaine and fulvic acid at the temperature of 23-27 ℃, and sowing after the seeds are exposed to white;
further preferably, a method for improving germination rate of aged seeds of flos Caryophylli by Co 60 The method comprises the steps of (1) radiating aged seeds of clove by gamma rays, controlling the radiation dose to be 20Gy, completely soaking the seeds in colchicine solution with the mass fraction of 0.05% for 5 hours after sun-drying, accelerating germination in sand containing a mixed solution of betaine and fulvic acid at the temperature of 23-27 ℃, and sowing after the seeds are exposed to white;
when germination accelerating treatment is carried out, the betaine is anhydrous betaine, the concentration of the betaine is 90mg/L, the concentration of fulvic acid is 5mg/L, the mixed solution of the betaine and the fulvic acid and river sand is stirred, the mixture is suitable for being not scattered by hand, and the mixture is not separated by water, and the same humidity is kept in the later water supplementing process; the germination accelerating temperature is kept at 23-27 ℃.
Compared with the prior art, the invention has the beneficial effects that:
(1) The invention adopts Co 60 The germination rate of the aged seeds of the clove is improved by combining gamma-ray radiation and colchicine solution soaking, and the germination rate of the aged seeds of the clove can be improved by 26.04% by adopting the method;
(2) The invention adopts a seed sunning treatment mode, which is favorable for breaking seed coats, so that colchicine, fulvic acid and betaine are easier to act in seeds, the use effect is improved, the use amount is reduced, and the cost is saved;
(3) The betaine and the fulvic acid are adopted, and have the effects of regulating the membrane permeability, improving the content of soluble sugar and the activity of antioxidant enzyme in plants, improving the activity of seeds, promoting the germination of the seeds and having remarkable beneficial effects after superposition and use.
Detailed Description
Specific embodiments of the present invention will now be described in order to provide a clearer understanding of the technical features, objects and effects of the present invention.
The germination accelerating and sowing time of examples 1-40 are all 4 late months, the ambient temperature is 25 ℃ during germination accelerating, sand humidity is suitable for the condition that the sand is not scattered by hand and is separated without water, the germination accelerating time is 4-6d, the sowing method, sowing density and sowing conditions of seeds are all kept the same, sowing is carried out after the seeds are exposed to white, the germination rate is counted, and the same batch of clove seeds are used.
Example 1
Placing fresh clove seeds in a refrigerator at 4 ℃ for 3 years, selecting 1500 clove seeds, carrying out germination accelerating treatment in wet sand containing clean water, timely supplementing water, sowing after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 55.24%.
Example 2
Placing fresh clove seeds in a refrigerator at 4 ℃ for 3 years, selecting 1500 clove seeds, fully soaking the seeds in clear water for 5 hours, then carrying out germination accelerating treatment in wet sand containing clear water, timely supplementing water, sowing after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 58.03%.
Example 3
Placing fresh clove seeds in a refrigerator at 4 ℃ for 3 years, selecting 1500 clove seeds, fully soaking the seeds in clear water for 10 hours, then carrying out germination accelerating treatment in wet sand containing clear water, timely supplementing water, sowing after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 62.78%.
Example 4
Placing fresh clove seeds in a refrigerator at 4 ℃ for 3 years, selecting 1500 clove seeds, fully soaking the seeds in clear water for 15 hours, accelerating germination in wet sand containing clear water, supplementing water in time, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 59.54%.
As can be seen from the results of examples 1 to 4, the aged seeds of Syzygium aromaticum were soaked with clear water before sowing, so that the germination rate could be improved, and the improvement value of the germination rate was the largest when the soaking time in clear water was 10 hours.
Example 5
Placing fresh clove seeds in a refrigerator at 4 ℃ for 3 years, selecting 1500 clove seeds, fully soaking the seeds in colchicine solution with the mass fraction of 0.05% for 5 hours, then accelerating germination in wet sand containing clear water, timely supplementing water, sowing after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 64.73%.
Example 6
Placing fresh clove seeds in a refrigerator at 4 ℃ for 3 years, selecting 1500 clove seeds, fully soaking the seeds in colchicine solution with the mass fraction of 0.10% for 5 hours, then carrying out germination accelerating treatment in wet sand containing clear water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 60.33%.
Example 7
Placing fresh clove seeds in a refrigerator at 4 ℃ for 3 years, selecting 1500 clove seeds, fully soaking the seeds in colchicine solution with the mass fraction of 0.30% for 5 hours, then carrying out germination accelerating treatment in wet sand containing clean water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 53.73%.
Example 8
Placing fresh clove seeds in a refrigerator at 4 ℃ for 3 years, selecting 1500 clove seeds, fully soaking the seeds in colchicine solution with the mass fraction of 0.50% for 5 hours, then carrying out germination accelerating treatment in wet sand containing clean water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 51.33%.
As can be seen from the germination rate results of examples 2, 5-8, the germination rate of example 5 was highest, i.e., the mass fraction optimum of the colchicine solution was 0.05%, and the difference between the germination rates of example 5 and example 2 was 6.70%, when the soaking time in the colchicine solution was 5 hours.
Example 9
Placing fresh clove seeds in a refrigerator at 4 ℃ for 3 years, selecting 1500 clove seeds, fully soaking the seeds in colchicine solution with the mass fraction of 0.05% for 10 hours, then carrying out germination accelerating treatment in wet sand containing clean water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 67.87%.
Example 10
Placing fresh clove seeds in a refrigerator at 4 ℃ for 3 years, selecting 1500 clove seeds, fully soaking the seeds in colchicine solution with the mass fraction of 0.10% for 10 hours, then carrying out germination accelerating treatment in wet sand containing clean water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 66.59%.
Example 11
Placing fresh clove seeds in a refrigerator at 4 ℃ for 3 years, selecting 1500 clove seeds, fully soaking the seeds in colchicine solution with the mass fraction of 0.30% for 10 hours, then carrying out germination accelerating treatment in wet sand containing clean water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 64.33%.
Example 12
Placing fresh clove seeds in a refrigerator at 4 ℃ for 3 years, selecting 1500 clove seeds, fully soaking the seeds in colchicine solution with the mass fraction of 0.50% for 10 hours, then carrying out germination accelerating treatment in wet sand containing clean water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 55.04%.
As can be seen from the results of the germination rates of examples 3 and 9 to 12, the germination rate of example 9 was highest when the soaking time in the colchicine solution was 10 hours, that is, the optimal value of the mass fraction of the colchicine solution was also 0.05%, and the difference between the germination rates of example 9 and example 3 was 5.09%.
Example 13
Placing fresh clove seeds in a refrigerator at 4 ℃ for 3 years, selecting 1500 clove seeds, fully soaking the seeds in colchicine solution with the mass fraction of 0.05% for 15 hours, then carrying out germination accelerating treatment in wet sand containing clean water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 52.79%.
Example 14
Placing fresh clove seeds in a refrigerator at 4 ℃ for 3 years, selecting 1500 clove seeds, fully soaking the seeds in colchicine solution with the mass fraction of 0.10% for 15 hours, then carrying out germination accelerating treatment in wet sand containing clean water, timely supplementing water, sowing after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 49.71%.
Example 15
Placing fresh clove seeds in a refrigerator at 4 ℃ for 3 years, selecting 1500 clove seeds, fully soaking the seeds in colchicine solution with the mass fraction of 0.30% for 15 hours, then carrying out germination accelerating treatment in wet sand containing clear water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 47.71%.
Example 16
Placing fresh clove seeds in a refrigerator at 4 ℃ for 3 years, selecting 1500 clove seeds, fully soaking the seeds in colchicine solution with the mass fraction of 0.50% for 15 hours, then carrying out germination accelerating treatment in wet sand containing clean water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 38.46%.
From the germination percentage results of examples 4, 13-16, it can be seen that the germination percentage of aged seeds of clove was inhibited when the soaking time in colchicine solution was 15 hours;
comparing the difference between the germination rates of example 5 and example 2 and the difference between the germination rates of example 9 and example 3, it was found that the difference between the germination rates of example 5 and example 2 was larger, indicating that the soaking time was controlled to 5 hours when the mass fraction of colchicine solution was 0.05%, and the germination rate was improved by 6.70%.
Example 17
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 And (3) radiating by gamma rays, controlling the radiation dose to be 20Gy, completely soaking the seeds in clear water for 10 hours after the radiation is finished, then carrying out germination accelerating treatment in wet sand containing clear water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate to be 52.33%.
Example 18
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 And (3) radiating by gamma rays, controlling the radiation dose to be 40Gy, completely soaking the seeds in clear water for 10 hours after the radiation is finished, then carrying out germination accelerating treatment in wet sand containing clear water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate to be 47.67%.
Example 19
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 The gamma rays are radiated, the radiation dosage is controlled to be 60Gy, the seeds are completely soaked in clear water for 10 hours after the radiation is finished, germination accelerating treatment is carried out in wet sand containing clear water, water supplementing is carried out in time, and the seeds are sowed and the germination rate is counted after the seeds are exposed to white, wherein the germination rate is 40.01%.
Example 20
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, and selecting 1500 flos CaryophylliSeed, co 60 And (3) radiating by gamma rays, controlling the radiation dose to be 80Gy, completely soaking the seeds in clear water for 10 hours after the radiation is finished, carrying out germination accelerating treatment in wet sand containing clear water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 36.02%.
Example 21
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 And (3) radiating by gamma rays, controlling the radiation dose to be 100Gy, completely soaking the seeds in clear water for 10 hours after the radiation is finished, carrying out germination accelerating treatment in wet sand containing clear water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 32.67%.
Example 22
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 And (3) radiating by gamma rays, controlling the radiation dose to be 120Gy, completely soaking the seeds in clear water for 10 hours after the radiation is finished, carrying out germination accelerating treatment in wet sand containing clear water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 27.01%.
Example 23
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 And (3) radiating by gamma rays, controlling the radiation dose to be 150Gy, completely soaking the seeds in clear water for 10 hours after the radiation is finished, carrying out germination accelerating treatment in wet sand containing clear water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 18.03%.
Example 24
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 And (3) radiating by gamma rays, controlling the radiation dose to be 170Gy, completely soaking the seeds in clear water for 10 hours after the radiation is finished, carrying out germination accelerating treatment in wet sand containing clear water, timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 10.33%.
Example 25
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 2 years, selecting 1500 flos Caryophylli seeds, and using Co 60 And (3) radiating by gamma rays, controlling the radiation dose to be 200Gy, completely soaking in clear water for 10 hours after the radiation is finished, carrying out germination accelerating treatment in wet sand containing clear water, timely supplementing water, sowing after the seeds are exposed to white, and counting the germination rate to be 1.21%.
As can be seen from the germination rate results of examples 1, 3 and 17 to 25, when Co is used 60 The germination rate of the aged seeds of clove decreases when irradiated by gamma rays, and the germination rate is reduced along with Co 60 An increase in the dose of gamma rays, the germination rate will be lower and lower, therefore, co 60 Gamma rays adversely affect germination of aged seeds of clove and Co 60 The larger the dose of gamma rays, the greater the adverse effect on the germination rate of the aged seeds of clove.
Example 26
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 Gamma-ray irradiation, controlling the irradiation dose to be 20Gy, and selecting a clear day within 1 week after the irradiation is finished to 8 am: 00 to 14:00, then, completely soaking the seeds in colchicine solution with the mass fraction of 0.05 percent for 5 hours, then, carrying out germination accelerating treatment in sand containing a mixed solution of betaine (90 mg/L) +fulvic acid (5 mg/L), and timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 81.28 percent.
Example 27
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 Gamma-ray irradiation, controlling the irradiation dose to be 60Gy, and selecting a clear day within 1 week after the irradiation is finished to 8 am: 00 to 14:00, then, completely soaking the seeds in colchicine solution with the mass fraction of 0.05 percent for 5 hours, then, carrying out germination accelerating treatment in sand containing a mixed solution of betaine (90 mg/L) +fulvic acid (5 mg/L), and timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 77.91 percent.
Example 28
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 Gamma-ray irradiation, controlling the irradiation dose to be 100Gy, and selecting a clear weather within 1 week after the irradiation is finished to 8 am: 00 to 14:00, then, completely soaking the seeds in colchicine solution with the mass fraction of 0.05 percent for 5 hours, then, carrying out germination accelerating treatment in sand containing a mixed solution of betaine (90 mg/L) +fulvic acid (5 mg/L), and timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 72.64 percent.
Example 29
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 Gamma-ray irradiation, controlling the irradiation dose to be 20Gy, and selecting a clear day within 1 week after the irradiation is finished to 8 am: 00 to 14:00, then, completely soaking the seeds in colchicine solution with the mass fraction of 0.1 percent for 5 hours, then, carrying out germination accelerating treatment in sand containing a mixed solution of betaine (90 mg/L) +fulvic acid (5 mg/L), and timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 74.50 percent.
Example 30
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 Gamma-ray irradiation, controlling the irradiation dose to be 60Gy, and selecting a clear day within 1 week after the irradiation is finished to 8 am: 00 to 14:00, then, completely soaking the seeds in colchicine solution with the mass fraction of 0.1 percent for 5 hours, then, carrying out germination accelerating treatment in sand containing a mixed solution of betaine (90 mg/L) +fulvic acid (5 mg/L), and timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 71.72 percent.
Example 31
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 Gamma-ray irradiation, controlling the irradiation dose to be 100Gy, and selecting a clear weather within 1 week after the irradiation is finished to 8 am: 00 to 14:00, and then the seeds are sun-cured at a mass fraction of 0.1%Soaking in colchicine solution for 5 hr, accelerating germination in sand containing mixed solution of betaine (90 mg/L) +fulvic acid (5 mg/L), supplementing water, sowing after the seeds are exposed, and counting germination rate, wherein the germination rate is 67.19%.
Example 32
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 Gamma-ray irradiation, controlling the irradiation dose to be 20Gy, and selecting a clear day within 1 week after the irradiation is finished to 8 am: 00 to 14:00, then, completely soaking the seeds in colchicine solution with the mass fraction of 0.3 percent for 5 hours, then, carrying out germination accelerating treatment in sand containing a mixed solution of betaine (90 mg/L) +fulvic acid (5 mg/L), and timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 65.08 percent.
Example 33
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 Gamma-ray irradiation, controlling the irradiation dose to be 60Gy, and selecting a clear day within 1 week after the irradiation is finished to 8 am: 00 to 14:00, then, completely soaking the seeds in colchicine solution with the mass fraction of 0.3 percent for 5 hours, then, carrying out germination accelerating treatment in sand containing a mixed solution of betaine (90 mg/L) +fulvic acid (5 mg/L), and timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 59.69 percent.
Example 34
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 Gamma-ray irradiation, controlling the irradiation dose to be 100Gy, and selecting a clear weather within 1 week after the irradiation is finished to 8 am: 00 to 14:00, then, completely soaking the seeds in colchicine solution with the mass fraction of 0.3 percent for 5 hours, then, carrying out germination accelerating treatment in sand containing a mixed solution of betaine (90 mg/L) +fulvic acid (5 mg/L), and timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 56.41 percent.
Example 35
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 Gamma-ray irradiation, controlling the irradiation dose to be 20Gy, and selecting a clear day within 1 week after the irradiation is finished to 8 am: 00 to 14:00, then, completely soaking the seeds in colchicine solution with the mass fraction of 0.05 percent for 10 hours, then, carrying out germination accelerating treatment in sand containing a mixed solution of betaine (90 mg/L) +fulvic acid (5 mg/L), and timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 70.71 percent.
Example 36
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 Gamma-ray irradiation, controlling the irradiation dose to be 60Gy, and selecting a clear day within 1 week after the irradiation is finished to 8 am: 00 to 14:00, then, completely soaking the seeds in colchicine solution with the mass fraction of 0.05 percent for 10 hours, then, carrying out germination accelerating treatment in sand containing a mixed solution of betaine (90 mg/L) +fulvic acid (5 mg/L), and timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 68.60 percent.
Example 37
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 Gamma-ray irradiation, controlling the irradiation dose to be 100Gy, and selecting a clear weather within 1 week after the irradiation is finished to 8 am: 00 to 14:00, then, completely soaking the seeds in colchicine solution with the mass fraction of 0.05 percent for 10 hours, then, carrying out germination accelerating treatment in sand containing a mixed solution of betaine (90 mg/L) +fulvic acid (5 mg/L), and timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 64.06 percent.
Example 38
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 Gamma-ray irradiation, controlling the irradiation dose to be 20Gy, and selecting a clear day within 1 week after the irradiation is finished to 8 am: 00 to 14:00, sun-drying seeds, and then adding autumn water with mass fraction of 0.05%Soaking in the solution of the Chinese herbal medicines for 15h, then carrying out germination accelerating treatment in sand containing a mixed solution of betaine (90 mg/L) +fulvic acid (5 mg/L), supplementing water in time, sowing after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 54.35%.
Example 39
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 Gamma-ray irradiation, controlling the irradiation dose to be 60Gy, and selecting a clear day within 1 week after the irradiation is finished to 8 am: 00 to 14:00, then, completely soaking the seeds in colchicine solution with the mass fraction of 0.05% for 15 hours, then, carrying out germination accelerating treatment in sand containing a mixed solution of betaine (90 mg/L) +fulvic acid (5 mg/L), and timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 52.07%.
Example 40
Placing fresh flos Caryophylli seeds in refrigerator at 4deg.C, storing for 3 years, selecting 1500 flos Caryophylli seeds, and using Co 60 Gamma-ray irradiation, controlling the irradiation dose to be 100Gy, and selecting a clear weather within 1 week after the irradiation is finished to 8 am: 00 to 14:00, then, completely soaking the seeds in colchicine solution with the mass fraction of 0.05% for 15 hours, then, carrying out germination accelerating treatment in sand containing a mixed solution of betaine (90 mg/L) +fulvic acid (5 mg/L), and timely supplementing water, sowing the seeds after the seeds are exposed to white, and counting the germination rate, wherein the germination rate is 48.52%.
As can be seen from the germination rate results of examples 1-4 and 26-40, co was obtained when the aged seeds of Caryophylli flos were immersed in the sun-seed and colchicine solution for 5 hours 60 The radiation dose of the gamma rays is 20Gy to 100Gy, the mass fraction of the colchicine solution is 0.05 percent to 0.1 percent, and after sand germination of the mixed solution containing betaine (90 mg/L) +fulvic acid (5 mg/L), the germination rate is promoted, and the promotion effect is along with Co 60 The increase in the dose of gamma rays was weaker, and the effect of example 26 on promoting germination was most remarkable as the mass fraction of the colchicine solution was increased, and the germination rate was increased by 26.04% as compared with example 1 and by 2 as compared with example 223.25%；
When the aged seeds of the clove are fully soaked in colchicine solution with the mass fraction of 0.3 percent for 5 hours under the conditions of sun-drying and germination accelerating as above, co 60 When the radiation dose of gamma rays is increased from 20Gy to 100Gy, the germination rate is changed from the promoting effect to the inhibiting effect, and when the germination rate is the promoting effect, the germination rate can be increased by 9.84% at most compared with the example 1, and the germination rate can be increased by 7.05% at most compared with the example 2;
when the aged seeds of the clove are fully soaked in colchicine solution with the mass percent of 0.05 percent for 10 hours under the conditions of sun-drying and germination accelerating as above, co 60 When the radiation dose of gamma rays is increased from 20Gy to 100Gy, the accelerating action on the germination rate becomes weaker gradually, and the germination rate can be increased by 15.47% at most compared with example 1, and 7.93% at most compared with example 3;
when the aged seeds of the clove are fully soaked in colchicine solution with the mass percent of 0.05 percent for 15 hours under the conditions of sun-drying and germination accelerating as above, co 60 When the radiation dose of gamma rays is increased from 20Gy to 100Gy, the germination rate is inhibited;
in conclusion, when Co 60 When the aged seeds of the clove are treated by combining gamma-ray radiation and colchicine solution soaking, the optimal treatment condition is Co 60 The dosage of gamma ray radiation is 20Gy, and the mixture is fully soaked in colchicine solution with the mass fraction of 0.05 percent for 5 hours, and the mixture is combined with the sun-dried seeds after the radiation and the wet Sha Cuiya of the colchicine soaking and then the mixture solution containing betaine (90 mg/L) +fulvic acid (5 mg/L), under the optimal treatment condition, the germination rate can be improved by 26.04 percent, and the germination rate can only be improved by 6.70 percent at most after the germination accelerating treatment by wet sand containing clean water when the colchicine solution is used alone for fully soaking treatment, so Co is used for preparing the fertilizer 60 The treatment mode of combining gamma radiation, sun-drying, colchicine soaking and germination accelerating by wet sand containing betaine and fulvic acid can play a remarkable role in promoting.
The percentages used in the present invention are mass percentages unless otherwise indicated.
Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present invention has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (2)
1. A method for improving germination rate of aged seeds of clove is characterized in that Co is used for the aged seeds of clove 60 The seeds are sun-dried after gamma-ray radiation, then are soaked by colchicine, then are put into sand containing mixed solution of betaine and fulvic acid for germination accelerating treatment, and are timely supplemented with water, and when the seeds are white after 4-6d, the white seeds are selected for sowing;
the clove aged seeds are clove seeds stored for 3 years at low temperature;
co use 60 The method comprises the steps of radiating the aged seeds of the clove by gamma rays, and completely soaking the seeds in colchicine solution with the mass fraction of 0.3% for 5 hours when the radiation dose is controlled to be 20-60 Gy;
co use 60 The method comprises the steps of radiating the aged seeds of the clove by gamma rays, and completely soaking the seeds in colchicine solution with the mass fraction of 0.05% for 10 hours when the radiation dose is controlled to be 20-100 Gy;
co use 60 The method comprises the steps of radiating the aged seeds of the clove by gamma rays, and completely soaking the seeds in colchicine solution with the mass fraction of 0.05-0.1% for 5 hours when the radiation dose is controlled to be 20-100 Gy;
when germination accelerating treatment is carried out, the betaine is anhydrous betaine, the concentration of the betaine is 90mg/L, the concentration of fulvic acid is 5mg/L, the mixed solution of the betaine and the fulvic acid and river sand is stirred, the mixture is suitable for being not scattered by hand, and the mixture is not separated by water, and the same humidity is kept in the later water supplementing process; the germination accelerating temperature is kept at 23-27 ℃.
2. The method for improving germination percentage of aged clove seeds according to claim 1, wherein the sun-drying is carried out within 1 week after the radiation treatment for 4 months and the following ten days, 8 am on sunny days is selected: 00 to 14: 00; and airing the irradiated seeds in the sun, wherein the paving thickness is suitable for the seeds not to be blocked.
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