CN117363662A - Saccharomyces cerevisiae fermentation product, preparation method and application thereof - Google Patents
Saccharomyces cerevisiae fermentation product, preparation method and application thereof Download PDFInfo
- Publication number
- CN117363662A CN117363662A CN202311306127.1A CN202311306127A CN117363662A CN 117363662 A CN117363662 A CN 117363662A CN 202311306127 A CN202311306127 A CN 202311306127A CN 117363662 A CN117363662 A CN 117363662A
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- CN
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- Prior art keywords
- fermentation
- fermentation product
- yeast
- saccharomyces cerevisiae
- parts
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- Granted
Links
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Classifications
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- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
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- A—HUMAN NECESSITIES
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- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
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Abstract
The invention provides a fermentation product of a saccharomyces cerevisiae, which is obtained by fermenting bifidobacteria, wherein the bifidobacteria are two of bifidobacterium adolescentis, bifidobacterium breve and bifidobacterium longum. The saccharomyces cerevisiae prepared by the invention has excellent DPPH removing effect, and has obvious effects of resisting wrinkles, tightening skin and relieving skin. Meanwhile, the prepared face cream has excellent use feeling on dry and oily skin, and cannot be excessively oiled or excessively dried.
Description
Technical Field
The invention relates to the technical field of daily chemicals, in particular to a yeast fermentation product, a preparation method and application thereof.
Background
At present, the fermentation product of the saccharomyces cerevisiae is produced after fermentation by bifidobacteria; the lysis of the fermentation product of the saccharomyces cerevisiae is a physiological lysate obtained by post-fermentation treatment of bifidobacterium, and a large number of in vitro experiments prove that various amino acids, proteins and various molecular mediums in the fermentation lysate of the bifidobacterium have the effects of regulating and balancing skin and regulating immune skills, and are also nutritional molecules of human skin cells.
The bifidobacterium fermentation lysate has certain anti-wrinkle, tightening, repairing, sun-screening, oxidation-resisting and aging-resisting effects, can prevent the damage caused by external stimulus such as ultraviolet rays and the like, and can promote the repair of damaged DNA. The yeast Saccharomyces cerevisiae produces extracellular polysaccharide during fermentation, which is a long-chain polysaccharide consisting of repeating units of monosaccharides or monosaccharide derivatives, which is long-chain, straight-chain or branched-chain structure, and which is analyzed by bacteria during growth metabolism to extracellular mucus or capsular polysaccharide.
During the fermentation process, bifidobacteria are anaerobic strains, and often need to face oxygen resistance challenges during the fermentation process, and resist oxygen poisoning effects. The components in the lysate of the fermentation product of the two-way yeast are also easily oxidized, so the lysate of the fermentation product of the two-way yeast should not be mixed with an oxidant for use, and meanwhile, the pH value of the lysate of the fermentation product of the two-way yeast is higher, generally about 7, and if the lysate of the fermentation product of the two-way yeast is mixed with an acidic substance for use, the pH value of the lysate of the fermentation product of the two-way yeast is possibly reduced, so that the efficacy of the lysate of the two-way yeast is affected.
Patent CN116656563A discloses a culture medium of the saccharomyces cerevisiae, a fermentation broth of the saccharomyces cerevisiae, and a preparation method and application thereof, and belongs to the technical field of cosmetics; the culture of the saccharomyces cerevisiae is carried out by adopting the saccharomyces cerevisiae culture medium provided by the invention, and the obtained saccharomyces cerevisiae fermentation liquid has excellent DPPH removing effect and obvious effects of resisting wrinkles, tightening skin and relieving skin. Meanwhile, the components of the culture medium of the saccharomyces cerevisiae are conveniently and easily obtained, and the preparation method of the fermentation liquid of the saccharomyces cerevisiae is simple to operate and is beneficial to actual production and application.
The invention provides a yeast fermentation product, a preparation method and application thereof.
Disclosure of Invention
The invention provides a fermentation product of a saccharomyces cerevisiae, which is obtained by fermenting bifidobacteria, wherein the bifidobacteria are two of bifidobacterium adolescentis, bifidobacterium breve and bifidobacterium longum.
The second aspect of the invention provides a preparation method of a yeast fermentation product, comprising the following steps:
s01, inoculating bifidobacteria into a seed culture medium for culture to obtain seed liquid;
s02, inoculating the seed solution into a fermentation medium for fermentation culture, and filtering after culture to obtain filtrate;
s03, carrying out alcohol precipitation treatment on the filtrate obtained in the step S02 to obtain a precipitate and a supernatant;
s04, inoculating the supernatant into a second fermentation medium for secondary fermentation, and then performing wall breaking treatment to obtain the fermentation product of the saccharomyces cerevisiae.
Preferably, the seed culture medium comprises the following components: 5.0g of soybean peptone, 5.0g of tryptone, 10.0g of yeast extract powder, 5.0g of glucose, 5.0g of D-lactose, 0.5g of L-cysteine hydrochloride, 801.0mL of Tween, 15.0g of agar, 40.0mL of salt solution, 1000.0mL of distilled water and CaCl 2 0.008g、MgSO 4 ·7H 2 O 0.00196g、K 2 HPO 4 0.04g、KH 2 PO 4 0.04g、NaHCO 3 0.04g、NaCl0.08g。
Preferably, the components of the fermentation medium in S02 are: 10g/L peptone, 5g/L liver extract powder, 3g/L beef extract powder, 5g/L yeast extract powder, 8g/L tryptone, 0.5g/L soluble starch, 1g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 10g/L glucose and 5g/L, mgSO sucrose 4 ·7H 2 O 0.01g/L，MnSO 4 0.005g/L, L-cysteine 0.5g/L, tween 801g/L.
Preferably, in S02, the fermentation conditions are: 37℃for 36h.
Preferably, in S04, the second medium comprises the following components: 11.0g/L of yeast extract, 10.0g/L of tryptone and 55.0g/L, caCl of glucose 2 0.04g/L, glucose 58.5g/L, mgSO 4 ·7H 2 0.3g/L of O, 1.2g/L of cysteine and 801mL/L of Tween.
The third aspect of the invention provides the yeast fermentation product which is applied to the field of daily chemicals.
The fourth aspect of the present invention provides a cream, which comprises: an oil phase component, an outer aqueous phase component, an inner emulsifier, and an outer emulsifier, wherein the inner aqueous phase component comprises the yeast fermentation product of claim 1.
Preferably, the internal emulsifier comprises the precipitate in step S03 in the fermentation of Saccharomyces cerevisiae according to claim 1.
In a fifth aspect, the present invention provides a skin care product comprising the fermentation product of Saccharomyces cerevisiae.
By adopting the technical scheme, the invention has the following beneficial effects:
the saccharomyces cerevisiae prepared by the invention has excellent DPPH removing effect, and has obvious effects of resisting wrinkles, tightening skin and relieving skin. Meanwhile, the prepared face cream has excellent use feeling on dry and oily skin, and cannot be excessively oiled or excessively dried.
Detailed Description
The following description of the present invention will be made clearly and fully, and it is apparent that the embodiments described are some, but not all, of the embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention provides a fermentation product of a saccharomyces cerevisiae, which is obtained by fermenting bifidobacteria, wherein the bifidobacteria are two of bifidobacterium adolescentis, bifidobacterium breve and bifidobacterium longum.
The second aspect of the invention provides a preparation method of a yeast fermentation product, comprising the following steps:
s01, inoculating bifidobacteria into a seed culture medium for culture to obtain seed liquid; wherein the inoculation amount is 4% (v/v), and the seed culture medium comprises the following components: 5.0g of soybean peptone, 5.0g of tryptone, 10.0g of yeast extract powder, 5.0g of glucose, 5.0g of D-lactose, 0.5g of L-cysteine hydrochloride, 801.0mL of Tween, 15.0g of agar, 40.0mL of salt solution, 1000.0mL of distilled water and CaCl 2 0.008g、MgSO 4 ·7H 2 O 0.00196g、K 2 HPO 4 0.04g、KH 2 PO 4 0.04g、NaHCO 3 0.04g、NaCl0.08g。
The seed culture condition is that the fermentation time is 12 hours at 37 ℃.
S02, inoculating the seed solution into a fermentation medium for fermentation culture, and filtering after culture to obtain filtrate; the inoculation amount of the seed liquid is 6% (v/v), and the components of the fermentation medium in the step S02 are as follows: peptone 10g/L, liver extract 5g/L, beef extract 3g/L, yeast extract 5g/L, and pancreas8g/L casein peptone, 0.5g/L soluble starch, 1g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 10g/L glucose and 5g/L, mgSO sucrose 4 ·7H 2 O0.01g/L，MnSO 4 0.005g/L, L-cysteine 0.5g/L, tween 801g/L. The fermentation conditions are as follows: 37℃for 36h.
S03, adding absolute ethyl alcohol with the same volume into the filtrate obtained in the step S02 to carry out alcohol precipitation treatment to obtain precipitate and supernatant respectively;
s04, inoculating the supernatant into a second fermentation medium, adding 0.1wt% of strain of the second fermentation medium, fermenting again, and performing wall breaking treatment to obtain the fermentation product of the saccharomyces cerevisiae. The second culture medium comprises the following components: 11.0g/L of yeast extract, 10.0g/L of tryptone and 55.0g/L, caCl of glucose 2 0.04g/L, glucose 58.5g/L, mgSO 4 ·7H 2 0.3g/L of O, 1.2g/L of cysteine and 1mL/L of Tween 80. The fermentation conditions are as follows: 37℃for 36h.
The wall breaking treatment mode is as follows: s04, centrifugally collecting thalli after fermentation, then adding distilled water with the same volume, and carrying out high-speed homogenizing wall breaking treatment under the ice bath condition, wherein the homogenizing wall breaking pressure is 120bar, and the temperature is-4 ℃.
The third aspect of the invention provides the yeast fermentation product which is applied to the field of daily chemicals.
The fourth aspect of the present invention provides a cream, which comprises: the yeast fermentation product comprises an oil phase component, an outer water phase component, an inner emulsifier and an outer emulsifier, wherein the inner water phase component comprises the yeast fermentation product.
Preferably, the cream ingredients comprise:
oil phase components: 4.5 parts of glycerol, 8 parts of caprylic/capric triglyceride, 4 parts of hydrogenated avocado oil, 3 parts of isononyl isononanoate, 0.01 part of ethylhexyl glycerol, 1.5 parts of polydimethylsiloxane, 0.3 part of tocopheryl acetate and 0.01 part of steareth-21;
external water phase components: 30 parts of water, 0.05 part of bisabolol, 0.01 part of phenoxyethanol, 0.003 part of xanthan gum, 0.005 part of trehalose, 0.03 part of sodium hydroxide, 0.28 part of polyacrylamide, 0.045 part of octanoyl hydroxamic acid, 0.05 part of xylitol, 0.03 part of octyl dodecanol and 0.02 part of EDTA disodium.
Internal aqueous phase composition: 25 parts of water, 0.05 part of N-palmitoyl hydroxyl proline cetyl ester, 0.1 part of sodium hyaluronate, 0.7 part of butanediol, 2 parts of nicotinamide, 0.05 part of a fermentation product of saccharomyces cerevisiae and 0.001 part of acetyl hexapeptide-8;
internal emulsifier: 3.2 parts of cetostearyl alcohol, 1.84 parts of hexyldecanol, 0.05 part of a fermentation precipitate of saccharomyces cerevisiae and 0.5 part of PEG-100 stearate;
external emulsifier: 0.6 part of cetostearyl glucoside, 0.05 part of stearic acid and 0.01 part of sodium lactate.
In another aspect, the invention provides a method of preparing the cream: the components in the components are respectively and evenly mixed; mixing the internal water phase component and the internal emulsifier, adding the oil phase component, and shearing to obtain water-in-oil colostrum; and mixing the water-in-oil colostrum and the external emulsifier solution, adding an external water phase component, shearing, and homogenizing to obtain the face cream.
In a fifth aspect, the present invention provides a skin care product comprising the fermentation product of Saccharomyces cerevisiae.
The preparation method comprises the steps of selecting a specific culture medium, collecting extracellular polysaccharide of the prepared yeast and using the extracellular polysaccharide in an emulsifier component, and adjusting the components of the culture medium to ensure that the yeast has certain compression resistance and oxidation resistance, so that the yeast has strong development capability, more trace elements, amino acids and other components are obtained in a human body, and in addition, the extracellular polysaccharide has more branched chains and can have better stabilizing effect in the cream in the later period of preparation. The cream prepared by the invention has the advantages that the extracellular polysaccharide is used as an adding step in the surfactant, so that the cream is stable, the performance is excellent, and the use feeling is proper.
The invention is further illustrated with reference to specific embodiments.
Bifidobacterium adolescentis: china center for type culture collection (CICC) 6179;
bifidobacterium longum: china center for type culture collection (CICC) 6187;
bifidobacterium breve: china center for type culture Collection of microorganisms, CICC6181.
Example 1
The embodiment provides a fermentation product of the saccharomyces cerevisiae, which is obtained by fermenting bifidobacteria, wherein the bifidobacteria are bifidobacterium adolescentis and bifidobacterium longum, and the inoculation ratio of the bifidobacterium adolescentis to the bifidobacterium longum is 1:1.
The second aspect of the present embodiment provides a method for preparing a yeast fermentation product, comprising the steps of:
s01, inoculating bifidobacteria into a seed culture medium for culture to obtain seed liquid; wherein the inoculation amount is 4% (v/v), and the seed culture medium comprises the following components: 5.0g of soybean peptone, 5.0g of tryptone, 10.0g of yeast extract powder, 5.0g of glucose, 5.0g of D-lactose, 0.5g of L-cysteine hydrochloride, 801.0mL of Tween, 15.0g of agar, 40.0mL of salt solution, 1000.0mL of distilled water and CaCl 2 0.008g、MgSO 4 ·7H 2 O 0.00196g、K 2 HPO 4 0.04g、KH 2 PO 4 0.04g、NaHCO 3 0.04g、NaCl 0.08g。
The seed culture condition is that the fermentation time is 12 hours at 37 ℃.
S02, inoculating the seed solution into a fermentation medium for fermentation culture, and filtering after culture to obtain filtrate; the inoculation amount of the seed liquid is 6% (v/v), and the components of the fermentation medium in the step S02 are as follows: 10g/L peptone, 5g/L liver extract powder, 3g/L beef extract powder, 5g/L yeast extract powder, 8g/L tryptone, 0.5g/L soluble starch, 1g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 10g/L glucose and 5g/L, mgSO sucrose 4 ·7H 2 O0.01g/L，MnSO 4 0.005g/L, L-cysteine 0.5g/L, tween 801g/L. The fermentation conditions are as follows: 37℃for 36h.
S03, adding absolute ethyl alcohol with the same volume into the filtrate obtained in the step S02 to carry out alcohol precipitation treatment to obtain precipitate and supernatant respectively;
s04 inoculating the supernatant to the second fermentation medium, and adding 0.1wt% of the secondFermenting the strain of the fermentation medium, and performing wall breaking treatment to obtain the fermentation product of the saccharomyces cerevisiae. The second culture medium comprises the following components: 11.0g/L of yeast extract, 10.0g/L of tryptone and 55.0g/L, caCl of glucose 2 0.04g/L、MgSO 4 ·7H 2 O0.3g/L, cysteine 1.2g/L, tween 801mL/L. The fermentation conditions are as follows: 37℃for 36h.
The wall breaking treatment mode is as follows: s04, centrifugally collecting thalli after fermentation, then adding distilled water with the same volume, and carrying out high-speed homogenizing wall breaking treatment under the ice bath condition, wherein the homogenizing wall breaking pressure is 120bar, and the temperature is-4 ℃.
The embodiment provides a face cream, comprising the following components:
oil phase components: 4.5 parts of glycerol, 8 parts of caprylic/capric triglyceride, 4 parts of hydrogenated avocado oil, 3 parts of isononyl isononanoate, 0.01 part of ethylhexyl glycerol, 1.5 parts of polydimethylsiloxane, 0.3 part of tocopheryl acetate and 0.01 part of steareth-21;
external water phase components: 30 parts of water, 0.05 part of bisabolol, 0.01 part of phenoxyethanol, 0.003 part of xanthan gum, 0.005 part of trehalose, 0.03 part of sodium hydroxide, 0.28 part of polyacrylamide, 0.045 part of octanoyl hydroxamic acid, 0.05 part of xylitol, 0.03 part of octyl dodecanol and 0.02 part of EDTA disodium.
Internal aqueous phase composition: 25 parts of water, 0.05 part of N-palmitoyl hydroxyl proline cetyl ester, 0.1 part of sodium hyaluronate, 0.7 part of butanediol, 2 parts of nicotinamide, 0.05 part of a fermentation product of saccharomyces cerevisiae and 0.001 part of acetyl hexapeptide-8;
internal emulsifier: 3.2 parts of cetostearyl alcohol, 1.84 parts of hexyldecanol, 0.05 part of a fermentation precipitate of saccharomyces cerevisiae and 0.5 part of PEG-100 stearate;
external emulsifier: 0.6 part of cetostearyl glucoside, 0.05 part of stearic acid and 0.01 part of sodium lactate.
In another aspect, the invention provides a method of preparing the cream: the components in the components are respectively and evenly mixed; mixing the internal water phase component and the internal emulsifier, adding the oil phase component, and shearing to obtain water-in-oil colostrum; and mixing the water-in-oil colostrum and the external emulsifier solution, adding an external water phase component, shearing, and homogenizing to obtain the face cream.
Example 2
The embodiment provides a fermentation product of the saccharomyces cerevisiae, which is obtained by fermenting bifidobacteria, wherein the bifidobacteria are bifidobacterium adolescentis and bifidobacterium longum, and the inoculation ratio of the bifidobacterium adolescentis to the bifidobacterium longum is 1:1.
The second aspect of the present embodiment provides a method for preparing a yeast fermentation product, comprising the steps of:
s01, inoculating bifidobacteria into a seed culture medium for culture to obtain seed liquid; wherein the inoculation amount is 4% (v/v), and the seed culture medium comprises the following components: 5.0g of soybean peptone, 5.0g of tryptone, 10.0g of yeast extract powder, 5.0g of glucose, 5.0g of D-lactose, 0.5g of L-cysteine hydrochloride, 801.0mL of Tween, 15.0g of agar, 40.0mL of salt solution, 1000.0mL of distilled water and CaCl 2 0.008g、MgSO 4 ·7H 2 O 0.00196g、K 2 HPO 4 0.04g、KH 2 PO 4 0.04g、NaHCO 3 0.04g、NaCl 0.08g。
The seed culture condition is that the fermentation time is 12 hours at 37 ℃.
S02, inoculating the seed solution into a fermentation medium for fermentation culture, and filtering after culture to obtain filtrate; the inoculation amount of the seed liquid is 6% (v/v), and the components of the fermentation medium in the step S02 are as follows: 10g/L peptone, 5g/L liver extract powder, 3g/L beef extract powder, 5g/L yeast extract powder, 8g/L tryptone, 0.5g/L soluble starch, 1g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 10g/L glucose and 5g/L, mgSO sucrose 4 ·7H 2 O 0.01g/L，MnSO 4 0.005g/L, L-cysteine 0.5g/L, tween 801g/L. The fermentation conditions are as follows: 37℃for 36h.
S03, carrying out wall breaking treatment on the filtrate obtained in the step S02 to obtain the fermentation product of the saccharomyces cerevisiae.
The wall breaking treatment mode is as follows: s03, centrifugally collecting thalli after fermentation, then adding distilled water with the same volume, and carrying out high-speed homogenizing wall breaking treatment under the ice bath condition, wherein the homogenizing wall breaking pressure is 120bar, and the temperature is-4 ℃.
The embodiment provides a face cream, comprising the following components:
oil phase components: 4.5 parts of glycerol, 8 parts of caprylic/capric triglyceride, 4 parts of hydrogenated avocado oil, 3 parts of isononyl isononanoate, 0.01 part of ethylhexyl glycerol, 1.5 parts of polydimethylsiloxane, 0.3 part of tocopheryl acetate and 0.01 part of steareth-21;
external water phase components: 30 parts of water, 0.05 part of bisabolol, 0.01 part of phenoxyethanol, 0.003 part of xanthan gum, 0.005 part of trehalose, 0.03 part of sodium hydroxide, 0.28 part of polyacrylamide, 0.045 part of octanoyl hydroxamic acid, 0.05 part of xylitol, 0.03 part of octyl dodecanol and 0.02 part of EDTA disodium.
Internal aqueous phase composition: 25 parts of water, 0.05 part of N-palmitoyl hydroxyl proline cetyl ester, 0.1 part of sodium hyaluronate, 0.7 part of butanediol, 2 parts of nicotinamide, 0.05 part of a fermentation product of saccharomyces cerevisiae and 0.001 part of acetyl hexapeptide-8;
internal emulsifier: 3.2 parts of cetostearyl alcohol, 1.84 parts of hexyldecanol and 0.5 part of PEG-100 stearate;
external emulsifier: 0.6 part of cetostearyl glucoside, 0.05 part of stearic acid and 0.01 part of sodium lactate.
In another aspect, the invention provides a method of preparing the cream: the components in the components are respectively and evenly mixed; mixing the internal water phase component and the internal emulsifier, adding the oil phase component, and shearing to obtain water-in-oil colostrum; and mixing the water-in-oil colostrum and the external emulsifier solution, adding an external water phase component, shearing, and homogenizing to obtain the face cream.
Example 3
The invention provides a yeast fermentation product of two cracks, which is obtained by fermenting bifidobacteria, wherein the bifidobacteria are bifidobacterium adolescentis and bifidobacterium longum, and the inoculation ratio of the bifidobacterium adolescentis to the bifidobacterium longum is 1:1.
The second aspect of the invention provides a preparation method of a yeast fermentation product, comprising the following steps:
s01, inoculating bifidobacteria into a seed culture medium for culture to obtain seed liquid; wherein the inoculation amount is 4% (v/v), and the seed culture medium comprises the following components: 5.0g of soybean peptone, 5.0g of tryptone, 10.0g of yeast extract powder, 5.0g of glucose, 5.0g of D-lactose and L-cysteine hydrochloride0.5g, tween 801.0mL, agar 15.0g, saline solution 40.0mL, distilled water 1000.0mL, caCl 2 0.008g、MgSO 4 ·7H 2 O 0.00196g、K 2 HPO 4 0.04g、KH 2 PO 4 0.04g、NaHCO 3 0.04g、NaCl 0.08g。
The seed culture condition is that the fermentation time is 12 hours at 37 ℃.
S02, inoculating the seed solution into a fermentation medium for fermentation culture, and filtering after culture to obtain filtrate; the inoculation amount of the seed liquid is 6% (v/v), and the components of the fermentation medium in the step S02 are as follows: 10g/L peptone, 5g/L liver extract powder, 3g/L beef extract powder, 5g/L yeast extract powder, 8g/L tryptone, 0.5g/L soluble starch, 1g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 10g/L glucose and 5g/L, mgSO sucrose 4 ·7H 2 O 0.01g/L，MnSO 4 0.005g/L, L-cysteine 0.5g/L, tween 801g/L. The fermentation conditions are as follows: 37℃for 36h.
S03, adding absolute ethyl alcohol with the same volume into the filtrate obtained in the step S02 to carry out alcohol precipitation treatment to obtain precipitate and supernatant respectively;
s04, inoculating the supernatant into a second fermentation medium, adding 0.1wt% of strain of the second fermentation medium, fermenting again, and performing wall breaking treatment to obtain the fermentation product of the saccharomyces cerevisiae. The second culture medium comprises the following components: 10g/L peptone, 5g/L liver extract powder, 3g/L beef extract powder, 5g/L yeast extract powder, 8g/L tryptone, 0.5g/L soluble starch, 1g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 10g/L glucose and 5g/L, mgSO sucrose 4 ·7H 2 O 0.01g/L，MnSO 4 0.005g/L, L-cysteine 0.5g/L, tween 801g/L. The fermentation conditions are as follows: 37℃for 36h.
The wall breaking treatment mode is as follows: s04, centrifugally collecting thalli after fermentation, then adding distilled water with the same volume, and carrying out high-speed homogenizing wall breaking treatment under the ice bath condition, wherein the homogenizing wall breaking pressure is 120bar, and the temperature is-4 ℃.
The embodiment provides a face cream, comprising the following components:
oil phase components: 4.5 parts of glycerol, 8 parts of caprylic/capric triglyceride, 4 parts of hydrogenated avocado oil, 3 parts of isononyl isononanoate, 0.01 part of ethylhexyl glycerol, 1.5 parts of polydimethylsiloxane, 0.3 part of tocopheryl acetate and 0.01 part of steareth-21;
external water phase components: 30 parts of water, 0.05 part of bisabolol, 0.01 part of phenoxyethanol, 0.003 part of xanthan gum, 0.005 part of trehalose, 0.03 part of sodium hydroxide, 0.28 part of polyacrylamide, 0.045 part of octanoyl hydroxamic acid, 0.05 part of xylitol, 0.03 part of octyl dodecanol and 0.02 part of EDTA disodium.
Internal aqueous phase composition: 25 parts of water, 0.05 part of N-palmitoyl hydroxyl proline cetyl ester, 0.1 part of sodium hyaluronate, 0.7 part of butanediol, 2 parts of nicotinamide, 0.05 part of a fermentation product of saccharomyces cerevisiae and 0.001 part of acetyl hexapeptide-8;
internal emulsifier: 3.2 parts of cetostearyl alcohol, 1.84 parts of hexyldecanol, 0.05 part of a fermentation precipitate of saccharomyces cerevisiae and 0.5 part of PEG-100 stearate;
external emulsifier: 0.6 part of cetostearyl glucoside, 0.05 part of stearic acid and 0.01 part of sodium lactate.
In another aspect, the invention provides a method of preparing the cream: the components in the components are respectively and evenly mixed; mixing the internal water phase component and the internal emulsifier, adding the oil phase component, and shearing to obtain water-in-oil colostrum; and mixing the water-in-oil colostrum and the external emulsifier solution, adding an external water phase component, shearing, and homogenizing to obtain the face cream.
Example 4
The embodiment provides a fermentation product of the saccharomyces cerevisiae, which is obtained by fermenting bifidobacteria, wherein the bifidobacteria are bifidobacterium adolescentis and bifidobacterium longum, and the inoculation ratio of the bifidobacterium adolescentis to the bifidobacterium longum is 1:1.
The second aspect of the present embodiment provides a method for preparing a yeast fermentation product, comprising the steps of:
s01, inoculating strains into a fermentation culture medium for fermentation culture, and filtering after culture to obtain filtrate; the fermentation medium in the step S02 comprises the following components: 10g/L peptone, 5g/L liver extract powder, 3g/L beef extract powder, 5g/L yeast extract powder, 8g/L tryptone, 0.5g/L soluble starch, 1g/L sodium chloride, 1g/L dipotassium hydrogen phosphate and monobasic phosphatePotassium 1g/L, glucose 10g/L, sucrose 5g/L, mgSO 4 ·7H 2 O 0.01g/L，MnSO 4 0.005g/L, L-cysteine 0.5g/L, tween 801g/L. The fermentation conditions are as follows: 37℃for 36h.
S03, adding absolute ethyl alcohol with the same volume into the filtrate obtained in the step S02 to carry out alcohol precipitation treatment to obtain precipitate and supernatant respectively;
s04, inoculating the supernatant into a second fermentation medium, adding 0.1wt% of strain of the second fermentation medium, fermenting again, and performing wall breaking treatment to obtain the fermentation product of the saccharomyces cerevisiae. The second culture medium comprises the following components: 11.0g/L of yeast extract, 10.0g/L of tryptone and 55.0g/L, caCl of glucose 2 0.04g/L、MgSO 4 ·7H 2 O0.3g/L, cysteine 1.2g/L, tween 801mL/L. The fermentation conditions are as follows: 37℃for 36h.
The wall breaking treatment mode is as follows: s04, centrifugally collecting thalli after fermentation, then adding distilled water with the same volume, and carrying out high-speed homogenizing wall breaking treatment under the ice bath condition, wherein the homogenizing wall breaking pressure is 120bar, and the temperature is-4 ℃.
The embodiment provides a face cream, comprising the following components:
oil phase components: 4.5 parts of glycerol, 8 parts of caprylic/capric triglyceride, 4 parts of hydrogenated avocado oil, 3 parts of isononyl isononanoate, 0.01 part of ethylhexyl glycerol, 1.5 parts of polydimethylsiloxane, 0.3 part of tocopheryl acetate and 0.01 part of steareth-21;
external water phase components: 30 parts of water, 0.05 part of bisabolol, 0.01 part of phenoxyethanol, 0.003 part of xanthan gum, 0.005 part of trehalose, 0.03 part of sodium hydroxide, 0.28 part of polyacrylamide, 0.045 part of octanoyl hydroxamic acid, 0.05 part of xylitol, 0.03 part of octyl dodecanol and 0.02 part of EDTA disodium.
Internal aqueous phase composition: 25 parts of water, 0.05 part of N-palmitoyl hydroxyl proline cetyl ester, 0.1 part of sodium hyaluronate, 0.7 part of butanediol, 2 parts of nicotinamide, 0.05 part of a fermentation product of saccharomyces cerevisiae and 0.001 part of acetyl hexapeptide-8;
internal emulsifier: 3.2 parts of cetostearyl alcohol, 1.84 parts of hexyldecanol, 0.05 part of a fermentation precipitate of saccharomyces cerevisiae and 0.5 part of PEG-100 stearate;
external emulsifier: 0.6 part of cetostearyl glucoside, 0.05 part of stearic acid and 0.01 part of sodium lactate.
In another aspect, the invention provides a method of preparing the cream: the components in the components are respectively and evenly mixed; mixing the internal water phase component and the internal emulsifier, adding the oil phase component, and shearing to obtain water-in-oil colostrum; and mixing the water-in-oil colostrum and the external emulsifier solution, adding an external water phase component, shearing, and homogenizing to obtain the face cream.
Example 5
The embodiment provides a fermentation product of the saccharomyces cerevisiae, which is obtained by fermenting bifidobacteria, wherein the bifidobacteria are bifidobacterium adolescentis and bifidobacterium longum, and the inoculation ratio of the bifidobacterium adolescentis to the bifidobacterium longum is 1:1.
The second aspect of the present embodiment provides a method for preparing a yeast fermentation product, comprising the steps of:
s01, inoculating bifidobacteria into a seed culture medium for culture to obtain seed liquid; wherein the inoculation amount is 4% (v/v), and the seed culture medium comprises the following components: 5.0g of soybean peptone, 5.0g of tryptone, 10.0g of yeast extract powder, 5.0g of glucose, 5.0g of D-lactose, 0.5g of L-cysteine hydrochloride, 1.0mL of Tween 80, 15.0g of agar, 40.0mL of saline solution, 1000.0mL of distilled water and CaCl 2 0.008g、MgSO 4 ·7H 2 O 0.00196g、K 2 HPO 4 0.04g、KH 2 PO 4 0.04g、NaHCO 3 0.04g、NaCl 0.08g。
The seed culture condition is that the fermentation time is 12 hours at 37 ℃.
S02, inoculating the seed solution into a fermentation medium for fermentation culture, and filtering after culture to obtain filtrate; the inoculation amount of the seed liquid is 6% (v/v), and the components of the fermentation medium in the step S02 are as follows: 10g/L peptone, 5g/L liver extract powder, 3g/L beef extract powder, 5g/L yeast extract powder, 8g/L tryptone, 0.5g/L soluble starch, 1g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 105g/L glucose and MgSO 4 ·7H 2 O0.01g/L，MnSO 4 0.005g/L, L-cysteine 0.5g/L, tween 801g/L. The fermentation conditions are as follows: 37℃for 36h.
S03, adding absolute ethyl alcohol with the same volume into the filtrate obtained in the step S02 to carry out alcohol precipitation treatment to obtain precipitate and supernatant respectively;
s04, inoculating the supernatant into a second fermentation medium, adding 0.1wt% of strain of the second fermentation medium, fermenting again, and performing wall breaking treatment to obtain the fermentation product of the saccharomyces cerevisiae. The second culture medium comprises the following components: 11.0g/L of yeast extract, 10.0g/L of tryptone and 55.0g/L, caCl of glucose 2 0.04g/L、MgSO 4 ·7H 2 O0.3g/L, cysteine 1.2g/L, tween 801mL/L. The fermentation conditions are as follows: 37℃for 36h.
The wall breaking treatment mode is as follows: s04, centrifugally collecting thalli after fermentation, then adding distilled water with the same volume, and carrying out high-speed homogenizing wall breaking treatment under the ice bath condition, wherein the homogenizing wall breaking pressure is 120bar, and the temperature is-4 ℃.
The embodiment provides a face cream, comprising the following components:
oil phase components: 4.5 parts of glycerol, 8 parts of caprylic/capric triglyceride, 4 parts of hydrogenated avocado oil, 3 parts of isononyl isononanoate, 0.01 part of ethylhexyl glycerol, 1.5 parts of polydimethylsiloxane, 0.3 part of tocopheryl acetate and 0.01 part of steareth-21;
external water phase components: 30 parts of water, 0.05 part of bisabolol, 0.01 part of phenoxyethanol, 0.003 part of xanthan gum, 0.005 part of trehalose, 0.03 part of sodium hydroxide, 0.28 part of polyacrylamide, 0.045 part of octanoyl hydroxamic acid, 0.05 part of xylitol, 0.03 part of octyl dodecanol and 0.02 part of EDTA disodium.
Internal aqueous phase composition: 25 parts of water, 0.05 part of N-palmitoyl hydroxyl proline cetyl ester, 0.1 part of sodium hyaluronate, 0.7 part of butanediol, 2 parts of nicotinamide, 0.05 part of a fermentation product of saccharomyces cerevisiae and 0.001 part of acetyl hexapeptide-8;
internal emulsifier: 3.2 parts of cetostearyl alcohol, 1.84 parts of hexyldecanol, 0.05 part of a fermentation precipitate of saccharomyces cerevisiae and 0.5 part of PEG-100 stearate;
external emulsifier: 0.6 part of cetostearyl glucoside, 0.05 part of stearic acid and 0.01 part of sodium lactate.
In another aspect, the invention provides a method of preparing the cream: the components in the components are respectively and evenly mixed; mixing the internal water phase component and the internal emulsifier, adding the oil phase component, and shearing to obtain water-in-oil colostrum; and mixing the water-in-oil colostrum and the external emulsifier solution, adding an external water phase component, shearing, and homogenizing to obtain the face cream.
Example 6
The embodiment provides a fermentation product of the saccharomyces cerevisiae, which is obtained by fermenting bifidobacteria, wherein the bifidobacteria are bifidobacterium adolescentis and bifidobacterium longum, and the inoculation ratio of the bifidobacterium adolescentis to the bifidobacterium longum is 1:1.
The second aspect of the present embodiment provides a method for preparing a yeast fermentation product, comprising the steps of:
s01, inoculating bifidobacteria into a seed culture medium for culture to obtain seed liquid; wherein the inoculation amount is 4% (v/v), and the seed culture medium comprises the following components: 5.0g of soybean peptone, 5.0g of tryptone, 10.0g of yeast extract powder, 5.0g of glucose, 5.0g of D-lactose, 0.5g of L-cysteine hydrochloride, 801.0mL of Tween, 15.0g of agar, 40.0mL of salt solution, 1000.0mL of distilled water and CaCl 2 0.008g、MgSO 4 ·7H 2 O 0.00196g、K 2 HPO 4 0.04g、KH 2 PO 4 0.04g、NaHCO 3 0.04g、NaCl 0.08g。
The seed culture condition is that the fermentation time is 12 hours at 37 ℃.
S02, inoculating the seed solution into a fermentation medium for fermentation culture, and filtering after culture to obtain filtrate; the inoculation amount of the seed liquid is 6% (v/v), and the components of the fermentation medium in the step S02 are as follows: 10g/L peptone, 5g/L liver extract powder, 3g/L beef extract powder, 5g/L yeast extract powder, 8g/L tryptone, 0.5g/L soluble starch, 1g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 10g/L glucose and 5g/L, mgSO sucrose 4 ·7H 2 O0.01g/L，MnSO 4 0.005g/L, L-cysteine 0.5g/L, tween 801g/L. The fermentation conditions are as follows: 37℃for 36h.
S03, adding absolute ethyl alcohol with the same volume into the filtrate obtained in the step S02 to carry out alcohol precipitation treatment to obtain precipitate and supernatant respectively;
s04, inoculating the supernatant into a second fermentation medium, adding 0.1wt% of strain of the second fermentation medium, fermenting again, and performing wall breaking treatment to obtain the fermentation product of the saccharomyces cerevisiae. The second culture medium comprises the following components: 11.0g/L of yeast extract, 10.0g/L of tryptone and 55.0g/L, caCl of glucose 2 0.04g/L、MgSO 4 ·7H 2 O0.3g/L, cysteine 1.2g/L, tween 801mL/L. The fermentation conditions are as follows: 37℃for 36h.
The wall breaking treatment mode is as follows: s04, centrifugally collecting thalli after fermentation, then adding distilled water with the same volume, and carrying out high-speed homogenizing wall breaking treatment under the ice bath condition, wherein the homogenizing wall breaking pressure is 120bar, and the temperature is-4 ℃.
The embodiment provides a face cream, comprising the following components:
oil phase components: 4.5 parts of glycerol, 8 parts of caprylic/capric triglyceride, 4 parts of hydrogenated avocado oil, 3 parts of isononyl isononanoate, 0.01 part of ethylhexyl glycerol, 1.5 parts of polydimethylsiloxane, 0.3 part of tocopheryl acetate and 0.01 part of steareth-21;
external water phase components: 30 parts of water, 0.05 part of bisabolol, 0.01 part of phenoxyethanol, 0.003 part of xanthan gum, 0.005 part of trehalose, 0.03 part of sodium hydroxide, 0.28 part of polyacrylamide, 0.045 part of octanoyl hydroxamic acid, 0.05 part of xylitol, 0.03 part of octyl dodecanol and 0.02 part of EDTA disodium.
Internal aqueous phase composition: 25 parts of water, 0.05 part of N-palmitoyl hydroxy proline cetyl ester, 0.1 part of sodium hyaluronate, 0.7 part of butanediol, 2 parts of nicotinamide, 0.05 part of a fermentation product of saccharomyces cerevisiae, 0.05 part of a fermentation precipitate of saccharomyces cerevisiae and 0.001 part of acetyl hexapeptide-8;
internal emulsifier: 3.2 parts of cetostearyl alcohol, 1.84 parts of hexyldecanol and 0.5 part of PEG-100 stearate;
external emulsifier: 0.6 part of cetostearyl glucoside, 0.05 part of stearic acid and 0.01 part of sodium lactate.
In another aspect, the invention provides a method of preparing the cream: the components in the components are respectively and evenly mixed; mixing the internal water phase component and the internal emulsifier, adding the oil phase component, and shearing to obtain water-in-oil colostrum; and mixing the water-in-oil colostrum and the external emulsifier solution, adding an external water phase component, shearing, and homogenizing to obtain the face cream.
Example 7
The embodiment provides a fermentation product of the saccharomyces cerevisiae, which is obtained by fermenting bifidobacteria, wherein the bifidobacteria are bifidobacterium adolescentis and bifidobacterium longum, and the inoculation ratio of the bifidobacterium adolescentis to the bifidobacterium longum is 1:1.
The second aspect of the present embodiment provides a method for preparing a yeast fermentation product, comprising the steps of:
s01, inoculating bifidobacteria into a seed culture medium for culture to obtain seed liquid; wherein the inoculation amount is 4% (v/v), and the seed culture medium comprises the following components: 5.0g of soybean peptone, 5.0g of tryptone, 10.0g of yeast extract powder, 5.0g of glucose, 5.0g of D-lactose, 0.5g of L-cysteine hydrochloride, 801.0mL of Tween, 15.0g of agar, 40.0mL of salt solution, 1000.0mL of distilled water and CaCl 2 0.008g、MgSO 4 ·7H 2 O 0.00196g、K 2 HPO 4 0.04g、KH 2 PO 4 0.04g、NaHCO 3 0.04g、NaCl0.08g。
The seed culture condition is that the fermentation time is 12 hours at 37 ℃.
S02, inoculating the seed solution into a fermentation medium for fermentation culture, and filtering after culture to obtain filtrate; the inoculation amount of the seed liquid is 6% (v/v), and the components of the fermentation medium in the step S02 are as follows: 10g/L peptone, 5g/L liver extract powder, 3g/L beef extract powder, 5g/L yeast extract powder, 8g/L tryptone, 0.5g/L soluble starch, 1g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 10g/L glucose and 5g/L, mgSO sucrose 4 ·7H 2 O0.01g/L，MnSO 4 0.005g/L, L-cysteine 0.5g/L, tween 801g/L. The fermentation conditions are as follows: 37℃for 36h.
S03, adding absolute ethyl alcohol with the same volume into the filtrate obtained in the step S02 to carry out alcohol precipitation treatment to obtain precipitate and supernatant respectively;
s04, inoculating the supernatant into a second fermentation medium, adding 0.1wt% of strain of the second fermentation medium, fermenting again, and performing wall breaking treatment to obtain a fermentation product of the Saccharomyces cerevisiae. The second culture medium comprises the following components: 11.0g/L of yeast extract, 10.0g/L of tryptone and 55.0g/L, caCl of glucose 2 0.04g/L、MgSO 4 ·7H 2 O0.3g/L, cysteine 1.2g/L, tween 801mL/L. The fermentation conditions are as follows: 37℃for 36h.
The wall breaking treatment mode is as follows: s04, centrifugally collecting thalli after fermentation, then adding distilled water with the same volume, and carrying out high-speed homogenizing wall breaking treatment under the ice bath condition, wherein the homogenizing wall breaking pressure is 120bar, and the temperature is-4 ℃.
The embodiment provides a face cream, comprising the following components:
oil phase components: 4.5 parts of glycerol, 8 parts of caprylic/capric triglyceride, 4 parts of hydrogenated avocado oil, 3 parts of isononyl isononanoate, 0.01 part of ethylhexyl glycerol, 1.5 parts of polydimethylsiloxane, 0.3 part of tocopheryl acetate and 0.01 part of steareth-21;
external water phase components: 30 parts of water, 0.05 part of bisabolol, 0.01 part of phenoxyethanol, 0.003 part of xanthan gum, 0.005 part of trehalose, 0.03 part of sodium hydroxide, 0.28 part of polyacrylamide, 0.045 part of octanoyl hydroxamic acid, 0.05 part of xylitol, 0.03 part of octyl dodecanol and 0.02 part of EDTA disodium.
Internal aqueous phase composition: 25 parts of water, 0.05 part of N-palmitoyl hydroxyl proline cetyl ester, 0.1 part of sodium hyaluronate, 0.7 part of butanediol, 2 parts of nicotinamide, 0.05 part of a fermentation product of saccharomyces cerevisiae and 0.001 part of acetyl hexapeptide-8;
internal emulsifier: 3.2 parts of cetostearyl alcohol, 1.84 parts of hexyldecanol, 0.05 part of a fermentation precipitate of saccharomyces cerevisiae and 0.5 part of PEG-100 stearate;
external emulsifier: 0.6 part of cetostearyl glucoside, 0.05 part of stearic acid and 0.01 part of sodium lactate.
In another aspect, the invention provides a method of preparing the cream: the components in the components are respectively and evenly mixed; mixing the inner water phase and the outer water phase, the inner emulsifier and the outer emulsifier, adding the oil phase component, shearing, and homogenizing to obtain the face cream.
Performance testing
Test 1: the creams prepared in examples 1 to 7 were placed at 0℃and 25℃and 40℃for 30d, respectively, and then the temperature was restored to room temperature, and the physical properties of the creams were frequently observed;
test 2: moisture retention test 24 female volunteers meeting the test conditions were selected for the test of examples 1-7 and blank control, 8 areas with a size of 2.0cm×2.0cm on the inner side of the left and right forearms of each volunteer, which were single-use products of subjects, 2mg/cm 2 Each test area was tested for skin moisture content before, 2 hours after and 8 hours after product use.
Test 3: antioxidant assay human immortalized keratinocytes were seeded in 96-well plates (2X 10) 4 /well), and culturing until the cell fusion degree is about 80% -90%. The medium in each well was aspirated and the positive control (PC, medium containing 0.05% VE) was added to the cream of examples 1-7 at a mass concentration of 1% in each well, incubated for 24 hours in an incubator, 100. Mu.L of DMEM medium containing DCFH-DA was added, incubated for 20 minutes in the incubator, washed 3 times with HBSS, and then added again with HBSS, and irradiated under UVA (dose 4.0J/cm) 2 ). And detecting the fluorescence intensity by a fluorescence enzyme-labeled instrument immediately after the irradiation is finished. The ROS levels for each group were calculated as 100% for the control group. UVA (320-400 nm) is adopted to induce and generate oxidation reaction, and an in-vitro cell oxidative stress model is constructed. Reactive Oxygen Species (ROS) levels were determined by analysis of the examples.
TABLE 1 test results of Performance test 1
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TABLE 2 test results of Performance test 2 (Table data is the average)
| Examples | Initial value | After 2 hours of using the product | After using the product for 8 hours |
| Example 1 | 38.14 | 62.18 | 56.27 |
| Example 2 | 37.93 | 50.13 | 43.29 |
| Example 3 | 37.37 | 56.46 | 50.25 |
| Example 4 | 38.14 | 58.12 | 52.22 |
| Example 5 | 38.01 | 59.82 | 54.15 |
| Example 6 | 37.34 | 60.25 | 52.18 |
| Example 7 | 37.56 | 59.36 | 48.25 |
| Blank space | 36.92 | 36.83 | 33.92 |
TABLE 3 test results of Performance test 3
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.
Claims (10)
1. A yeast fermentation product is characterized in that the yeast fermentation product is obtained by fermenting bifidobacteria, wherein the bifidobacteria are two of bifidobacterium adolescentis, bifidobacterium breve and bifidobacterium longum.
2. The method for producing a yeast fermentation product according to claim 1, comprising the steps of:
s01, inoculating bifidobacteria into a seed culture medium for culture to obtain seed liquid;
s02, inoculating the seed solution into a fermentation medium for fermentation culture, and filtering after culture to obtain filtrate;
s03, carrying out alcohol precipitation treatment on the filtrate obtained in the step S02 to obtain a precipitate and a supernatant;
s04, inoculating the supernatant into a second fermentation medium for secondary fermentation, and then performing wall breaking treatment to obtain the fermentation product of the saccharomyces cerevisiae.
3. The method for producing a yeast fermentation product according to claim 2, wherein the seed medium comprises the following components: 5.0g of soybean peptone, 5.0g of tryptone, 10.0g of yeast extract powder, 5.0g of glucose, 5.0g of D-lactose, 0.5g of L-cysteine hydrochloride, 1.0mL of Tween 80, 15.0g of agar, 40.0mL of saline solution, 1000.0mL of distilled water and CaCl 2 0.008g、MgSO 4 ·7H 2 O0.00196g、K 2 HPO 4 0.04g、KH 2 PO 4 0.04g、NaHCO 3 0.04g、NaCl0.08g。
4. The method for producing a fermentation product of Saccharomyces cerevisiae according to claim 2, wherein the fermentation medium in S02 comprises the following components: 10g/L peptone, 5g/L liver extract powder, 3g/L beef extract powder, 5g/L yeast extract powder, 8g/L tryptone, 0.5g/L soluble starch, 1g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 1g/L potassium dihydrogen phosphate, 10g/L glucose and 5g/L, mgSO sucrose 4 ·7H 2 O0.01g/L，MnSO 4 0.005g/L, L-cysteine 0.5g/L, tween 801g/L.
5. The method for producing a yeast fermentation product according to claim 2, wherein the fermentation conditions in S02 are: 37℃for 36h.
6. The method for producing a yeast fermentation product according to claim 2, wherein in S04, the secondThe culture medium comprises the following components: 11.0g/L of yeast extract, 10.0g/L of tryptone and 55.0g/L, caCl of glucose 2 0.04g/L, glucose 58.5g/L, mgSO 4 ·7H 2 O0.3g/L, cysteine 1.2g/L, tween 801mL/L.
7. The yeast fermentation product of claim 1, which is used in the field of daily chemicals.
8. A facial cream, characterized in that the preparation raw materials comprise: an oil phase component, an outer aqueous phase component, an inner emulsifier, and an outer emulsifier, wherein the inner aqueous phase component comprises the yeast fermentation product of claim 1.
9. A cream as claimed in claim 8 wherein the internal emulsifier comprises the precipitate of step S03 in the fermentation of the yeast of claim 1.
10. A skin care product comprising the fermentation product of saccharomyces cerevisiae according to claim 1.
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